scholarly journals Biberiye (Rosmarinus officinalis L.) Uçucu Yağının Tavuk Yumurtalarının Kolesterol ve Yağ Asitleri Düzeyi Üzerine Etkileri

Author(s):  
Tülay Çimrin ◽  
Murat Demirel

This experiment was carried out to investigate the effects of variant doses of rosemary (Rosmarinus officinalis L.) essential oils (REO), vitamin E (α‐tocopheryl acetate) and antibiotic (chlortetracyclin) on the concentration of vitamin E, fatty acid composition, cholesterol levels in the yolk. In this experiment, 32 weeks old Bovans genotype and 240 white laying hens were randomly separated to six treatment groups with five replicas. While the control group was fed with basal diet the treatment groups were supplemented with 500 mg/kg antibiotics; 200 mg/kg vitamin E; 100, 200, and 300 mg/kg REO to basal diet for 90 consecutive days. The results showed that addition of antibiotics, vitamin E and REO to the diet had no effects on egg cholesterol, while fatty acid composition and vitamin E contents of the yolk were significantly affected. Addition of 100 mg/kg REO to the diet increased total mono unsaturated fatty acids (MUFA) by increasing oleic acid level but decreased Polyunsaturated fatty acids (PUFA) by decreasing linoleic acid content of the yolk. Addition of 200 mg/kg vitamin E to the diet significantly increased vitamin E content of egg. The study showed that the changes in yolk fatty acid composition and vitamin E content due to the feed additives, could affect positively in chemical composition of eggs. In conclusion, 100 mg/kg REO and 200 mg/kg vitamin E supplementation to the diet might cause high antioxidant capacity and could help to produce eggs that were more resistant to lipid oxidation.

2010 ◽  
Vol 25 (1) ◽  
pp. 45-54 ◽  
Author(s):  
H.D. Karsten ◽  
P.H. Patterson ◽  
R. Stout ◽  
G. Crews

AbstractIn the US farmers often market pastured poultry eggs for a premium price, claiming animal and human health benefits. We examined how moving pastured hens to forage legumes or mixed grasses influenced hen (Gallus gallusL.) egg omega-3 fatty acids and concentrations of vitamins A and E. We also compared the eggs of the pastured hens to those of hens fed a commercial diet in cages. We used a cross-over design to compare pasture species: 75 sister hens were assigned to one of three pasture treatment groups: (1) alfalfa (Medicago sativaL.), (2) red and white clover (Trifolium pretenseL. andTrifolium repensL.) or (3) mixed cool season grasses. Groups were rotated to all three pasture treatments, each for 2 weeks and supplemented with 70 g commercial hen mash bird−1day−1. Pasture botanical composition, forage mass, leaf to total ratio and plant fatty acid composition were compared among pasture treatments. Eggs of the pastured hens were compared to eggs of 50 sister hens that were fed only commercial hen mash in cages for the entire 6 weeks. Forage parameters varied somewhat, but did not explain plant linolenic acid variation. Seventeen of the 18 quantified egg fatty acids, and vitamin A concentrations did not (P<0.05) differ among the three pasture treatment groups. Eggs of the hens that foraged grasses had 23% more (P<0.0001) vitamin E than eggs of hens that foraged clover. Compared to eggs of the caged hens, pastured hens' eggs had twice as much vitamin E and long-chain omega-3 fats, 2.5-fold more total omega-3 fatty acids, and less than half the ratio of omega-6:omega-3 fatty acids (P<0.0001). Vitamin A concentration was 38% higher (P<0.05) in the pastured hens' eggs than in the caged hens' eggs, but total vitamin A per egg did not differ. At the end of the experiment, pastured hens weighed 14% less and averaged 15% lower hen-day egg production than caged birds (P<0.0001). Results suggest that grass pastures may enhance vitamin E in eggs of pastured hens more than clover, and pastured hens supplemented with commercial mash will produce eggs with significantly more vitamin E and total omega-3 fatty acids compared to eggs from caged hens fed only commercial hen mash. Pastured hens may have lower body weight and egg production than caged hens, unless they are supplemented adequately to meet their dietary energy and crude protein needs.


1996 ◽  
Vol 1996 ◽  
pp. 30-30
Author(s):  
C.O. Leskanich ◽  
K.R. Matthews ◽  
C.C. Warkup ◽  
R.C. Noble

The tissues of animals for food use have come to be associated with a predominance of saturated and monounsaturated fatty acids, the result of which has been to contribute to the perceived human dietary imbalance of fatty acids. The fact that porcine tissues assume a fatty acid composition similar to that of the respective diet has enabled the composition to be altered with respect to human dietary needs (Morgan et al, 1992). The fatty acid compositions of rapeseed and fish oils are characterised by a number of factors of relevance to human health recommendations (BNF, 1992). Thus, rapeseed oil contains a low content of saturates, a moderate content of linoleic acid and a high content of α-linolenic acid whilst fish oil contains high levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Although benefiting the nutritional value of meat/fat, the feeding of increased levels of highly polyunsaturated fatty acids has the potential, in theory at least, of adversely affecting organoleptic and various physical properties. Such adverse effects could be manifested during and/or after the preparation and cooking of the meat or meat products at which times the oxidative degradation of fatty acids is maximised. The inclusion of dietary vitamin E has a range of beneficial effects on meat quality principally due to its antioxidant effects. The present experiment was an attempt to optimise the fatty acid composition of pork and pork products for human health purposes whilst not adversely affecting factors controlling consumer acceptability.


1999 ◽  
Vol 1999 ◽  
pp. 115-115 ◽  
Author(s):  
A.M. Wachira ◽  
L.A. Sinclair ◽  
R.G. Wilkinson ◽  
G. Demirel ◽  
M. Enser ◽  
...  

The benefits of long chain polyunsaturated fatty acids (PUFA) to human health, especially those of the n-3 series are now widely recognised. In a previous experiment (Wachira et al. 1998) supplementing diets with whole linseed or fish oil increased n-3 fatty acid levels in lamb muscle. To raise these further the whole linseed can be treated with formaldehyde to increase protection in the rumen. Dietary antioxidants such as vitamin E can control lipid oxidation but information on their effects on lamb performance and fatty acid composition is limited. The current experiments investigated the effects of different dietary PUFA sources and vitamin E levels on growth and fatty acid composition in two sheep breeds. Detailed results of the effects of vitamin E are presented in the accompanying abstract by Enser et al.


Reproduction ◽  
2001 ◽  
pp. 315-322 ◽  
Author(s):  
JA Rooke ◽  
CC Shao ◽  
BK Speake

The aim of the present study was to characterize the effects of feeding tuna oil on the lipid and fatty acid composition of boar spermatozoa and to relate changes in composition to boar semen characteristics. Ten boars were paired by age and allocated to one of two diets (five boars per diet). The diets, which were offered for 6 weeks, consisted of a basal diet that was either unsupplemented or supplemented with 30 g tuna oil kg(-1) diet. Adding tuna oil to the diet increased the ether extract concentration of the diets fed from 65 to 92 g kg(-1) dry matter and supplied 10.5 g long chain polyunsaturated (n-3) fatty acids per 100 g total fatty acids. There were no changes in semen fatty acid composition after 3 weeks of feeding tuna oil. However, after 5 and 6 weeks, the proportions (g per 100 g total fatty acids) of 22:6(n-3) in sperm phospholipid fatty acids were increased from 34.5 to 42.9 g by feeding tuna oil and 22:5(n-6) decreased from 29.8 to 17.9 g. No changes were observed in other sperm lipids or seminal plasma phospholipids as a result of the diets fed. Feeding tuna oil increased the proportion of spermatozoa with progressive motility and with a normal acrosome score and reduced the proportion of spermatozoa with abnormal morphologies.


2008 ◽  
Vol 88 (3) ◽  
pp. 475-483 ◽  
Author(s):  
Maziar Mohiti-Asli ◽  
Farid Shariatmadari ◽  
Houshang Lotfollahian ◽  
Mohamad Taghi Mazuji

A 7-wk trial was carried out to investigate the effect of vitamin E and inorganic and organic selenium added to hens' diet on quality and lipid stability of eggs during storage. One hundred forty-four Hy-Line W-36 hens (63-wk of age) were divided into six equal groups. Five groups received a basal diet supplemented with 0.4 mg kg-1 sodium selenite or selenium yeast, 200 mg kg-1 vitamin E or a combination of selenium and vitamin E; whereas the control group received no supplementation. Hen production was assessed daily and fresh egg quality parameters were determined every 2 wk. Eggs were stored under different conditions (4°C, 23–27°C or 31°C) for 14 d. Eggs were analyzed for quality characteristics, egg component weight, Malondialdehyde values as a secondary oxidation product and yolk fatty acid (FA) composition. The performance of the hens and egg weights were not affected either by the source of the selenium or by the vitamin E. The inclusion of selenium or vitamin E in the diet significantly increased their concentrations in the egg. The supplemented diets also improved egg quality, oxidative stability and fatty acid composition during storage. Key words: Vitamin E, selenium, lipid peroxidation, egg fatty acid composition, storage


2017 ◽  
Vol 57 (9) ◽  
pp. 1899
Author(s):  
Xubiao Wei ◽  
Xiudong Liao ◽  
Jun Cai ◽  
Zhaojun Zheng ◽  
Lulu Zhang ◽  
...  

An experiment was conducted to investigate the effects of Bacillus amyloliquefaciens LFB112 in the diet on growth of broilers and on the quality and fatty acid composition of broiler meat. 440 1-day-old Arbor Acres male broiler chicks were randomly allocated into five dietary treatments with eight replicates in a completely randomised design for 42 days. The treatments were as follows: basal diet (control) or the basal diet supplemented with 107 colony-forming units (CFU) B. amyloliquefaciens/kg (B7), 108 CFU B. amyloliquefaciens/kg (B8), 109 CFU B. amyloliquefaciens/kg (B9) or 40 mg aureomycin/kg (antibiotic). The results showed that B. amyloliquefaciens-supplemented groups had greater (P < 0.05) average daily gain during Days 1 to 21, Days 22 to 42 and Days 1 to 42, and improved (P < 0.05) feed conversion rate during Days 1 to 42 compared with the control. Broilers in the B7 and B9 groups had higher (P < 0.05) average daily feed intake than those in the control group in all experimental phases. Broilers fed either B. amyloliquefaciens or antibiotic diet showed higher (P < 0.05) a* value, b* value and lower L* value in the breast muscle than those in the control group. Dietary B. amyloliquefaciens supplementation increased (P < 0.05) the contents of C14:1, C16:1c, C18:1t, C18:1c, C20:1n-9, total monounsaturated fatty acids, C18:2c, C18:3n-3, C20:2n-6, C20:3n-6, C20:3n-3, C20:4n-6, C20:5n-3, C22:6n-3 and total polyunsaturated fatty acids as well as ratio of polyunsaturated fatty acids to saturated fatty acids in breast muscle and the concentrations of C17:1, C20:1n-9, C18:2t-9, t-12, C20:2n-6, C20:3n-6, C20:3n-3, C20:4n-6, C20:5n-3 and C22:6n-3 in thigh muscle of broilers. The results indicated that B. amyloliquefaciens improved broilers’ growth performance, meat quality and fatty acid composition.


2019 ◽  
Vol 88 (4) ◽  
Author(s):  
Agnieszka Trela ◽  
Grażyna Silska ◽  
Marek Chyc ◽  
Dariusz Latowski ◽  
Jerzy Kruk ◽  
...  

Flax, Linum usitatissimum, cultivars are grown throughout the world. Flax oil is a dietary source of polyunsaturated fatty acids, vitamin E, as well as phospholipids, sterols, and phenolic acids. Linseed plays a pivotal role in protecting cells from oxidative damage associated diseases, i.e., atherosclerosis, neurodegenerative disorders, cancer, and inflammation. In this study, two groups of L. usitatissimum seeds were used to evaluate and compare the content and composition of tocochromanols (vitamin E) and fatty acids. Group I included accessions originating from Poland and the Ukraine, while Group II encompassed worldwide flax cultivars (such as from the United States, Argentina, and Italy). A comparison of the tocochromanol profiles showed a higher content in Group I, although there were no significant differences in tocopherol content and composition between the genotypes within this group. All accessions in Groups I and II contained γ-tocotrienol and plastochromanol-8, which confirms the high nutritional value of flaxseeds. The composition of fatty acids varied depending on the varieties, with linolenic acid showing the greatest discrepancy. Based on the tocochromanol content and fatty acid composition, we conducted a principal component analysis (PCA) and cluster analysis, which revealed a greater similarity among the accessions in Group I. An analysis of the tocochromanol and fatty acid composition of flaxseeds is important from an agronomic and medicinal perspective and can be used to select the most appropriate flax cultivar.


2008 ◽  
Vol 20 (1) ◽  
pp. 128
Author(s):  
C. A. A. Torres ◽  
E. A. M. Amorim ◽  
L. S. Amorim ◽  
J. K. Graham ◽  
M. Meyers

In most mammals, docosahexaenoic acid (DHA) is the dominant polyunsaturated fatty acid (PUFA), although, in several species, docosapentaenoic acid is also a major component of the sperm cell membranes. The amount of DHA in spermatozoa is positively correlated with sperm motility. The effect of dietary supplementation with tuna oil (TO) on the lipid and fatty acid composition of boar spermatozoa and the relationship between the changes in composition and boar semen characteristics were studied. Twenty-four boars were distributed in a completely randomized factorial design (2 � 3) with two oil sources (soybean and tuna) and three levels of antioxidant (150, 300, and 450 mg of vitamin E/kg). The diets consisted of a basal diet that was supplemented with 30 g soybean or TO per kg diet. During a period of 10 weeks of feeding the diets, one ejaculate from each boar was collected per week. The sperm was diluted 1:1 with Beltsville thawing solution (BTS) and divided into three portions destined to cooling (5 and 17�C) and freezing. The sperm diluted for cooling at either temperature was stored in plastic bottles for 3 days. After dilution with BTS, the sperm for freezing was centrifuged and rediluted with freezing extender before it was stored in 0.5-mL straws. Thawing was achieved by placing the straws in a water bath (37�C) for 30 s. Motility, vigor, hypoosmotic swelling (host), and morphology were assessed. For determining the fatty acid composition of the spermatozoa and seminal plasma, a sample of 15 mL was taken from each ejaculate shortly after collection and centrifuged for 20 min at 1000g. Sperm motility and vigor were analyzed by placing a sample on a pre-warmed (37�C) microscopic slide, covering with a coverslip, and examining under a light microscope at a magnification of 200�. For host assessment in each case, a volume of 10 µL was mixed with 1 mL hypoosmotic solution (100 mOsm L–1) and incubated for 30 min in a water bath (37�C). After incubation, 50 µL of formol-saline was added to each tube. Sequentially, 20 µL of every sample was smeared on a microscope slide and observed with oil immersion using a phase contrast microscope. A minimum of 200 cells was observed and classified as non-coiled and coiled. Lipid peroxidation was measured using the thiobarbituric acid reaction. Treatment differences for sperm were determined using analysis of variance for means. The proportion of DHA in sperm phospholipid fatty acids increased in semen fatty acid composition after 1 week of feeding TO. The concentrations of the fatty acids were unchanged in the seminal plasma as a result of the diets fed. The proportion of spermatozoa with abnormal morphologies decreased in boars supplemented with TO (P < 0.05). The TO diet showed the lowest level of total antioxidants in the semen (P < 0.05); however, when the diet was supplemented with the higher vitamin E level, an increase in sperm motility and vigor was observed (P < 0.05). Therefore, dietary supplementation with TO alters the lipid composition of the membrane and has a beneficial effect on both cooled and cryopreserved boar spermatozoa by decreasing cold shock during cooling and thus increasing cryosurvival.


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