scholarly journals Uji Efek Antibakteri Minyak Jintan Hitam (Nigella Sativa) Dalam Kapsul yang Dijual Bebas Selama Tahun 2012 di Kota Padang Terhadap Bakteri Staphylococcus aureus dan Escherichia coli Secara In Vitro

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Gezha Utama Putra ◽  
Aziz Djamal ◽  
Machdawaty Masri
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Nigella Sativa ◽  
Antibacterial Effects ◽  
Anova Analysis ◽  
Black Cumin ◽  
Germ Free ◽  
Free Area

AbstrakJintan Hitam atau Nigella sativa diketahui memiliki kemampuan sebagai antibakteri, seperti terhadap Staphylococcus aureus dan Escherichia coli. Kedua bakteri ini memiliki sifat yang berbeda namun memiliki kesamaan dari penyakit yang disebabkannya. Tujuan penelitian ini adalah untuk membandingkan efek antibakteri produk minyak jintan hitam (Nigella sativa) dalam bentuk kapsul antara bakteri Staphylococcus aureus dan Escherechia coli secara in vitro. Penelitian ini dilakukan pada bulan Januari 2012 - Desember 2012 di Laboratorium Mikrobiologi, Fakultas Kedokteran Universitas Andalas. Penelitian ini menunjukkan bahwa semua sampel jintan hitam yang digunakan tidak memiliki efek antibakteri terhadap Escherichia coli dan semua sampel tersebut memiliki efek antibakteri yang bervariasi pada Staphylococcus aureus. Analisis Anova menunjukkan bahwa masing-masing sampel minyak Nigella sativa memberikan efek berbeda secara signifikan pada pertumbuhan Staphylococcus aureus, bahkan enam diantaranya memiliki efek yang lebih baik daripada Cepoperazon (25 mm) sebagai kontrol yang digunakan.Kata kunci: Nigella sativa, efek antibakteri, Staphylococcus aureus, Escherichia coli. AbstractBlack Cumin or Nigella sativa known have the capability as antibacterial, such as to Staphylococcus aureus and Escherichia coli. Both of these bacteria have different properties but have similarities from diseases they may cause. The objective of this study was to compare anti-bacterial effects of black cumin oil (Nigella sativa) which has been packaged in capsules towards Staphylococcus aureus and Escherichia coli in vitro The research was conducted in January 2012 to December 2012 at the Microbiology laboratory, Medicine Faculty of Andalas University. The results of this research indicate that all black cumin samples that are used did not have antibacterial effect against Escherichia coli and all samples had antibacterial effects on Staphylococcus aureus with germ-free area that size varies. Anova analysis results indicate that each of Nigella sativa gives differ effects significantly on Staphylococcus aureus growth, even six of them have better effect than Cepoperazon (25 mm) were used as controls.Keywords: Nigella sativa, antibacterial effect, Staphylococcus aureus, Escherichia coli

scholarly journals Perbandingan Efek Antibakteri Madu Asli Sikabu dengan Madu Lubuk Minturun terhadap Escherichia Coli dan Staphylococcus Aureus secara In Vitro

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Yugo Berri Putra Rio ◽  
Aziz Djamal ◽  
Asterina Asterina
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Honey Bees ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Antibacterial Effects ◽  
Traditional Medicines ◽  
The Common ◽  
And Staphylococcus Aureus

AbstrakPendahuluan: Madu digunakan sebagai agen makanan dan obat tradisional, mengandung nektar atau gulaeksudat dari tanaman yang dikumpulkan oleh lebah madu serta merupakan salah satu obat tradisional yangdigunakan oleh masyarakat. Madu diketahui memiliki kemampuan sebagai efek antibakteri, seperti Escherichia colidan Stahpylococcus aureus. Kedua bakteri ini memiliki sifat yang berbeda tetapi memilki kesamaan dari penyakityang disebabkannya. Kualitas dan jenis madu juga sesuai dengan tempat dan lokasi lebah berkembang biak.Tujuan penelitian ini adalah untuk melihat perbandingan efek antibakteri madu asli Sikabu dan Lubuk Minturunterhadap Escherichia coli dan Staphylococcus aureus secara in vitro. Metode Penelitian: Madu yang diuji untukpenelitian ini adalah madu yang berasal dari Sikabu dan Lubuk Minturun. Penelitian ini dilaksanakan pada bulanDesember 2011 sampai Mei 2012 di laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Jenispenelitian yang digunakan adalah eksperimental dengan menggunakan metode difusi (metode cakram) dananalitik dengan menghubungkan perbedaan yang dimiliki kedua madu sebagai efek antibakteri. Hasil Penelitian:ini menunjukkan bahwa madu asli Sikabu dan Lubuk Minturun tidak memiliki kemampuan efek antibakteri terhadapEscherichia coli, dan terdapat perbedaan efek antibakteri dari kedua madu terhadap Staphylococcus aureus.Kesimpulan: dari kedua jenis madu yang diteliti tidak ditemukan efek antibakteri terhadap Escherichia coli , namunmadu asli Sikabu memiliki efek antibakteri yang lebih baik dari pada madu Lubuk Minturun terhadapStaphylococcus aureusKata Kunci : Perbandingan Efek Antibakteri, Madu Asli Sikabu, Madu Lubuk Minturun. Escherichia coli,Staphylococcus aureusAbstractIntroduction: Honeyis used as an agent for food and traditional medicine, containing nectar or sugar exudates ofthe plant collected by honey bees, is one of the traditional medicines used by community. Honey is known to havethe ability as an antibacterial effect, such as Escherichia coli and Stahpylococcus aureus. Both of these bacteriahave different properties but have the common of the diseases it causes. The quality and type of honey is also inaccordance with the breeding places and their location. The purpose of this study was to see a comparison of theantibacterial effect of honey Sikabu and Lubuk Minturun against Escherichia coli and Staphylococcus aureus by invitro. Methods: Honey to be tested for this study were derived from Sikabu and Lubuk Minturun. This study wasconducted in December 2011 to May 2012 in the laboratory of Microbiology, Faculty of Medicine, AndalasUniversity. Type of research is experimental diffusion method (disc method) and analytical distinction owned bylinking the two of honey as an antibacterial effect. Results: These results indicate that the honey Sikabu and LubukMinturun have no antibacterial effect against Escherichia coli, both of honey have differences antibacterial effectagainst Staphylococcus aureus. Conclusion: Both types of honey from this study did not find an antibacterial effectagainst Escherichia coli, but honey Sikabu has better antibacterial against Staphylococcus aureus than honeyLubuk Minturun.Keywords: Comparisons Antibacterial Effects, Original Sikabu Honey, Honey Lubuk Minturun. Escherichia coli,Staphylococcus aureus

scholarly journals Antibacterial Effect of Autologous Platelet-Rich Gel Derived from Subjects with Diabetic Dermal Ulcers In Vitro

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Lihong Chen ◽  
Chun Wang ◽  
Hengchuan Liu ◽  
Guanjian Liu ◽  
Xingwu Ran
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Effect ◽  
Control Group ◽  
Diabetic Patients ◽  
Antibacterial Effects ◽  
Significant Difference ◽  
Autologous Platelet

Background. Autologous platelet-rich gel (APG) is an effective method to improve ulcer healing. However, the mechanisms are not clear. This study aimed to investigate the antibacterial effect of APG in vitro.Methods. Platelet-rich plasma (PRP), platelet-poor plasma (PPP) and APG were prepared from whole blood of sixteen diabetic patients with dermal ulcers. Antibacterial effects againstStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosawere evaluated by bacteriostasis assay of APG, PRP, and APG-APO (APG combined with apocynin), with phosphate-buffered saline (PBS) and PPP as the control group.Results. (1) Compared to the PBS and PPP, the APG and APG-APO groups showed strong antibacterial activity againstStaphylococcus aureus. There was no significant difference(P>0.05)between APG and APG-APO. (2) Compared to PBS, APG, APG-APO, and PRP showed obvious antibacterial effects againstEscherichia coliandPseudomonas aeruginosa. No significant difference(P>0.05)was revealed among the three groups. Compared to the PPP group, they did not show antibacterial effect againstEscherichia coliandPseudomonas aeruginosa(P>0.05).Conclusions. APG has antibacterial effect againstStaphylococcus aureusmediated by platelet activation in the diabetic patients with dermal ulcer, and does not present obvious antibacterial effect againstEscherichia coliorPseudomonas aeruginosa. Combination of APG and antibiotics may have synergistic antibacterial effect.

scholarly journals Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Nursenem Karaca ◽  
Görkem Şener ◽  
Betül Demirci ◽  
Fatih Demirci
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Antibacterial Effect ◽  
Synergistic Effects ◽  
Pharmaceutical Formulations ◽  
Human Pathogens ◽  
Broth Microdilution ◽  
Antibacterial Effects ◽  
Lavandula Latifolia

AbstractCombination of various compounds and essential oils for pharmaceutical formulations withdraw attention. In this present study, it was aimed to evaluate the in vitro potential synergistic antibacterial effect of Lavandula latifolia (spike lavender) essential oil with camphor by using the checkerboard method against the human pathogens; Staphylococcus aureus and Listeria monocytogenes. Pharmacopoeia quality L. latifolia essential oil and racemic camphor were analyzed and verified by GC-FID and GC/MS, simultaneously. In vitro antibacterial activity of essential oil and camphor (MIC range: 0.16–20 mg/mL) and standard antimicrobial clarithromycin (MIC range: 0.125–16 μg/mL) were carried out by broth microdilution against S. aureus and L. monocytogenes standard strains, respectively. Resulting antibacterial effects were evaluated for their fractional inhibitory concentrations (FICs) as antagonistic, additive and synergistic effects. The analytical results showed that the major component of essential oil was linalool (45.2%) and 1,8-cineole (25.6%). Antibacterial effects of essential oil were determined as MIC 1.25–5 mg/mL. As a result of the experiments, L. latifolia essential oil–camphor combinations were identified as “synergistic (FIC ≤ 0.5), and additive (0.5 < FIC ≤ 1)” in the respective combinations, suggesting further evaluation for formulations for potential antimicrobial applications in food and pharmaceuticals.

scholarly journals Antibacterial Properties of Nanosilver PLLA Fibrous Membranes

2009 ◽  
Vol 2009 ◽  
pp. 1-5 ◽  
Author(s):  
Lin Li ◽  
Yi Li ◽  
Jiashen Li ◽  
Lei Yao ◽  
Arthur F. T. Mak ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Weight Ratio ◽  
Antibacterial Effects ◽  
E Coli ◽  
Fibrous Membranes ◽  
Composite Fibrous Membranes

Nanosilver has been studied as a valuable material for it strong antibacterial effects. In this study, we investigated the antibacterial properties of nano silver Poly-L-Lactic acid (Ag/PLLA) composite fibrous membranes. Ag/PLLA fibrous membranes were prepared with silver nanoparticles having weight ratio of silver nanoparticles to PLLA at 5% (w/w). In vitro antibacterial tests were performed usingEscherichia coli(E. coli) andStaphylococcus aureus(Staph.) to determine the antibacterial capability of the Ag/PLLA fibrous membranes. As the results suggested, Ag/PLLA fibrous membranes showed strong antibacterial properties. Thus, Ag/PLLA fibrous membrane can be used as an antibacterial scaffold for tissue engineering.

scholarly journals Uji Efek Antibakteri Chromodoris dianae terhadap Bakteri Escherichia coli dan Staphylococcus aureus

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Eflentina Kipimbob ◽  
Robert Bara ◽  
Pemsi M. Wowor ◽  
Jimmy Posangi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Experimental Study ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Positive Control ◽  
Antibacterial Effects ◽  
E Coli ◽  
Mean Diameter ◽  
The Mean

Abstract: This study was aimed to evaluate the antibacterial effect of Chromodoris dianae on E.coli and S. aureus. This was an experimental study. Samples of Chromodoris dianae was taken from Bunaken waters by diving. Extract of Chromodoris dianae was obtained by using maceration technique with 96% etanol. Antibacterial activity of this extract was tested by using the Kirby-Bauer method. The results showed that the mean diameter of the inhibition zone of E. coli was 22.3±1.5 mm and of S. aureus was 23.0±1.0 mm; both were were less than of ciprofloxacin as the positive control repeated for three times. Conclusionn: Chromodoris dianae has antibacterial effects on the growth of Escherichia coli and Staphylococcus aureus.Keywords: Chromodoris dianae, Staphylococcus aureus, Escherichia coli Abstrak: Penelitian ini bertujuan untuk melihat adanya efek antibakteri dari Chromodoris dianae terhadap bakteri E.coli dan S. aureus. Jenis penelitian ialah eksperimental. Sampel Chromodoris dianae diambil dari perairan Bunaken dengan cara menyelam. Ekstrak Chromodoris dianae dibuat dengan cara maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan menggunakan metode Kirby-Bauer. Hasil penelitian menunjukkan adanya zona hambat terhadap E. coli dan S. aureus dengan rerata diameter 22,3±1,5 mm dan 23,0±1,0 mm, yang lebih kecil daripada rerata diameter kontrol positif siprofloksasin pada tiga kali pengulangan. Simpulan: Chromodoris dianae memiliki efek antibakteri terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli.Kata kunci: Chromodoris dianae, Staphylococcus aureus, Escherichia coli

scholarly journals Uji Efek Antibakteri Ekstrak Etanol Kulit Batang Salam {Syzigium polyanthum (Wight) Walp} terhadap Staphylococcus aureus dan Escherichia coli secara Invitro

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Ikhsan Amanda Putra ◽  
Erly Erly ◽  
Machdawaty Masri
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Descriptive Study ◽  
Effective Concentration ◽  
Diffusion Method ◽  
Antibacterial Effects ◽  
Activity Test ◽  
Growth Of Bacteria

AbstrakTumbuhan salam (Syzygium polyanthum (Wight) Walp.) telah dikenal sejak dahulu untuk mengobati berbagai penyakit. Bagian tumbuhan yang dapat dimanfaatkan sebagai obat selain daun adalah bagian kulit batang. Beberapa penelitian yang telah dilakukan terbukti bahwa daun salam memiliki efek antibakteri. Tujuan penelitian ini adalah untuk mengetahui bagaimana efek antibakteri dari kulit batang salam. Penelitian ini bersifat deskriptif menggunakan metode difusi agar. Konsentrasi ekstrak yang digunakan adalah 25%, 50%, 75%, dan 100%. Hasil penelitian menunjukkan bahwa ekstrak etanol kulit batang salam memiliki efek antibakteri terhadap Staphylococcus aureus dengan konsentrasi 25%, 50%, 75%, dan 100% memberikan daya hambat terhadap pertumbuhan bakteri sebesar 12 mm, 13,67 mm, 12,33 mm, dan 9 mm, sedangkan pada konsentrasi yang sama untuk Escherichia coli tidak terlihat daya hambat terhadap pertumbuhan bakteri. Konsentrasi ekstrak yang paling efektif dalam menghambat S. aureus adalah konsentrasi 50%, dimana konsentrasi 75% dan 100% kurang efektif.Kata kunci: uji efek antibakteri, kulit batang salam, staphylococcus aureus, eschericia coli AbstractSalam plants (Syzygium polyanthum (Wight) Walp.) Salam plants have been known since ancient to treat various diseases. The parts of the plant that can be used as drug are bark. From the research that has been conducted has proven that Salam leaves has an antibacterial effect. The objective of this study was to determine the antibacterial effect of salam bark.This was a descriptive study by using agar diffusion method. The concentration of the extract used was 25%, 50%, 75%, and 100%. The results showed that the ethanol extract of Salam bark has antibacterial effects to Staphylococcus aureus with the concentration of 25%, 50%, 75%, and 100% gives the inhibition of the growth of bacteria on 12 mm, 13.67 mm, 12.33 mm, and the 9 mm, while at the same concentration for Escherichia coli was no bacterial inhibition area. The most effective concentration of extract in inhibiting S. aureus was concentration of 50%, while the concentration of 75% and 100% less effective.Keywords: antibacterial activity test, salam bark, staphylococcus aureus, eschericia coli

scholarly journals The Effectiveness of Giving Natural Honey as a Bactericide Against the Growth of Escherichia coli Bacteria

2021 ◽  
Vol 11 (3) ◽  
pp. 183-187
Author(s):  
Mulyadi Djojosaputro ◽  
Moskwadina Gultom
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Antibacterial Effects ◽  
Clear Zone ◽  
Activity Test ◽  
Escherichia Coli Bacteria ◽  
Natural Honey

Honey is one of the natural treatments with antibacterial effects such as hydrogen peroxide, low pH and short water activity that may inhibit bacterial growth. This study aimed to determine the activity of honey as antibacterial to bacteria Escherichia coli. Antibacterial activity test was done in vitro through diffusion method by using disk disc in MHA media then measuring the diameter of a clear zone that inhibition zone form growth of Escherichia coli bacteria and scaled it millimetre unit. This research was done in the laboratory of the Faculty of Medicine Universitas Kristen Indonesia. The concentrations used are 10%, 30%, 50%, 70%, 100% by using sterile equates as honey diluents. The control used was chloramphenicol, the results obtained at a concentration of 10% with a zone of 7.6 mm inhibition of honey had been able to inhibit the growth of Escherichia coli bacteria, and the optimum concentration was 100% with a zone of 24.5 mm. This test showed that natural honey could inhibit bacteria, which conclude that honey might be used as alternative medicine in society. Keywords: Antibacterial effect, Escherichia coli, honey

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