Prunella vulgaris L. seeds: a promising source of lipids, proteins, and original phenolic compounds presenting high antioxidant and anti-inflammatory activity

Description of the subject. Prunella vulgaris L. (Lamiaceae) is an edible herb used in Europe and Asia as herbal tea, food, dietary supplement or medicinal plant since several centuries. Although the composition and properties of the aerial parts are well documented in the literature, Prunella vulgaris seeds have never been investigated. Objectives. The aim of this work was to characterize the composition of these seeds to assess not only their nutritional potential (protein, lipid and minerals content), but also their antioxidant and anti-inflammatory potential as well as their specialized metabolites composition. Method. The protein, lipids and mineral content were determined. The phenolic compounds were isolated; elucidation of their structures was done by means of HR-MS and one- and two-dimensional NMR analysis. They were then evaluated for their biological activity, together with seed extracts. Results. Omega-3 rich oil and an interesting amino acids profile were detected in the seeds as well as the presence of a high content of polyphenolic compounds, including two structurally new compounds (phenylpropane derivatives) which were isolated and structurally characterized. The two compounds described here for the first time, were named amolsamic acids A&B. The seeds and amolsamic acids showed an interesting antioxidant potential, and a strong anti-inflammatory activity on neutrophils, MPO and HRP models. Conclusions. These findings highlight the real health promoting potential of P. vulgaris seeds and make them a good candidate as super-food or food supplement.

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Elsholtzia ciliata (Thunb.) Hyl. Extracts from Different Plant Parts: Phenolic Composition, Antioxidant, and Anti-Inflammatory Activities

Molecules ◽

10.3390/molecules25051153 ◽

2020 ◽

Vol 25 (5) ◽

pp. 1153 ◽

Cited By ~ 5

Author(s):

Lauryna Pudziuvelyte ◽

Mindaugas Liaudanskas ◽

Aiste Jekabsone ◽

Ilona Sadauskiene ◽

Jurga Bernatoniene

Keyword(s):

Phenolic Compounds ◽

Prostaglandin E2 ◽

High Performance ◽

Inflammatory Activity ◽

Antioxidant Efficiency ◽

Plant Parts ◽

Ethanolic Extracts ◽

Elsholtzia Ciliata ◽

Health Promoting ◽

Anti Inflammatory

Polyphenols play an important role on the health-promoting properties of humans. Plants belonging to Lamiaceae family are known as rich source of phenolic compounds. The current work aimed to evaluate the phenolic compounds, antioxidant, and anti-inflammatory activity of Elsholtzia ciliata (Thunb.) Hyl. ethanolic extracts from leaf, stem, flower, and whole herb. Twelve compounds were identified in ethanolic extracts using high-performance liquid chromatography (HPLC). The HPLC analysis revealed that chlorogenic acid, rosmarinic acid, and rutin were predominant compounds in ethanolicic extracts. Using HPLC-ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) post-column assay, avicularin, chlorogenic, and rosmarinic acids were identified as the predominant radical scavengers in all ethanolic extracts. All tested preparations significantly reduced the level of secretion of proinflammatory cytokines TNF-α, IL-6, and prostaglandin E2 induced by lipopolysaccharide treatment in mouse peritoneal macrophage cell culture. Stem and flower extracts were most efficient in reducing cytokine release, but leaf extract demonstrated stronger effect on prostaglandin E2 secretion. This is the first study exploring antioxidant efficiency by HPLC–ABTS post-column method and investigating anti-inflammatory activity of ethanolic extracts from E. ciliata different plant parts.

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In-vivo anti-inflammatory activity and safety assessment of the aqueous extract of Algerian Erica arborea L. (Ericaceae) aerial parts

Journal of Ethnopharmacology ◽

10.1016/j.jep.2021.113881 ◽

2021 ◽

Vol 271 ◽

pp. 113881

Author(s):

Djouher Amroun ◽

Meriem Hamoudi ◽

Seddik Khennouf ◽

Sabrina Boutefnouchet ◽

Daoud Harzallah ◽

...

Keyword(s):

Aqueous Extract ◽

Safety Assessment ◽

Inflammatory Activity ◽

Aerial Parts ◽

Erica Arborea ◽

Anti Inflammatory

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ANTIULCER AND ANTI-INFLAMMATORY ACTIVITIES OF HYDROALCOHOLIC EXTRACTS OF STEM BARK AND LEAF OF ANOGEISSUS PENDULA EDGEW

INDIAN DRUGS ◽

10.53879/id.58.01.11950 ◽

2021 ◽

Vol 58 (01) ◽

pp. 41-46

Author(s):

Deeksha Singh ◽

◽

Amit Nayak ◽

Rakesh Yadav ◽

Uttam Singh Baghel ◽

...

Keyword(s):

Phenolic Compounds ◽

Stem Bark ◽

Inflammatory Activity ◽

Antiulcer Activity ◽

Paw Edema ◽

Ulcer Index ◽

Pylorus Ligation ◽

Anti Inflammatory ◽

Anogeissus Pendula

Anogeissus pendula Edgew has been reported to be used in gastric disorder even though no attempt has been made to evaluate the same. The present study was designed to evaluate the hydroalcoholic extracts of stem bark and leaves for in vivo acute antiulcer and anti-inflammatory activities. Antiulcer activity was studied by pylorus ligation induced ulcers while anti-inflammatory activities was studied by carrageenan induced paw edema. The extract of stem bark at 200 and 400 mg/kg b. w., significantly (P<0.05) reduced ulcer index whereas extracts of both stem bark and leaves significantly (P<0.001) reduced paw diameter at the fifth hour. The hydroalcholic extract of stem bark of A. pendula possesses more significant antiulcer and anti-inflammatory activity than the hydroalcholic extract of leaves may be due to the presence of higher amount of phenolic compounds.

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Chemical Constituents from Farfugium Japonicum Var. formosanum

Natural Product Communications ◽

10.1177/1934578x1200700405 ◽

2012 ◽

Vol 7 (4) ◽

pp. 1934578X1200700 ◽

Cited By ~ 1

Author(s):

Sung-Fei Hsieh ◽

Tain-Jye Hsieh ◽

Mohamed El-Shazly ◽

Ying-Chi Du ◽

Chin-Chung Wu ◽

...

Keyword(s):

Chemical Constituents ◽

Cancer Cell Line ◽

Inflammatory Activity ◽

Human Neutrophils ◽

Spectral Techniques ◽

Methanolic Extracts ◽

Aerial Parts ◽

Anti Inflammatory ◽

Farfugium Japonicum ◽

First Time

Two new eremophilenolides, 6β8β10β-trihydroxyeremophil-7(11)-en-12,8-olide (1) and 3βacetoxy-8α-hydroxy-6β-methoxyeremophila-7(11),9-dien-12,8-olide (2) along with twenty-nine other compounds were obtained from the methanolic extracts of the aerial parts and rhizomes of Farfugium japonicum (L.) Kitam. v ar. formosanum (Hayata) Kitam. (Compositae = Asteraceae). The structures of the isolated compounds were characterized and identified by spectral techniques. Compounds 5, 6, 10, 12-24, 29, and 30, were reported for the first time from this genus. Cytotoxicity and anti-inflammatory activity of the isolated compounds were evaluated. Compounds 3 and 16 possessed moderate cytotoxicity against human breast cancer cell line (MCF 7). Compounds 3, 16, 25 and 26 exhibited moderate cytotoxicity against hepatoma cells (Hep G2 and Hep 3B). With respect to the anti-inflammatory activity, compounds 15 and 16 (each 10 μg/mL) inhibited superoxide anion generation by human neutrophils in response to fMLP/CB by 92.0% and 87.3%, respectively.

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ASSESMENT OF ANTI-INFLAMMATORY ACTIVITY AND CYTOTOXICITY OF FREEZE DRIED HYDROALCOHOLIC EXTRACT OF Bidens andicola ON ISOLATED NEUTROPHILS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i6.17574 ◽

2017 ◽

Vol 10 (6) ◽

pp. 160

Author(s):

Vinueza D ◽

LÓpez E ◽

Acosta K ◽

Abdo S

Keyword(s):

Freeze Drying ◽

Water Soluble ◽

Inflammatory Activity ◽

Hydroalcoholic Extract ◽

Cytotoxicity Assays ◽

Aerial Parts ◽

Controlled Conditions ◽

Freeze Dried ◽

Anti Inflammatory

Objective: The aim of this study was to evaluate anti-inflammatory activity and cytotoxicity in vitro of hydroalcoholic extract of Bidens andicola.Methods: B. andicola hydroalcoholic extract was obtained from aerial parts of B. andicola, following a standardized methodology. Briefly, aerial parts of B. andicola were extracted with ethanol 70% v/v and defatted with n-hexane, hydroalcoholic fraction was concentrated under controlled conditions in a rotary evaporator, and finally the residue was freeze-drying to obtain the hydroalcoholic extract of B. andicola. Anti-inflammatory activity and cytotoxicity assays were carried out using in vitro isolated neutrophils model using stable water-soluble tetrazolium salts.Results and Conclusions: The in vitro anti-inflammatory assay on isolated neutrophils demonstrated that the hydroalcoholic extract showed antiinflammatoryactivity compared to aspirin, with inflammatory inhibition percent values of 80.138±0.729 to hydroalcoholic extract of B. andicola and 82.117±0.762 to aspirin, each tested in five replicates at the concentration of 200 ppm of hydroalcoholic extract or reference.

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Terpenoid and phenolic derivatives from the aerial parts of Elsholtzia rugulosa and their anti-inflammatory activity

Phytochemistry ◽

10.1016/j.phytochem.2020.112543 ◽

2021 ◽

Vol 181 ◽

pp. 112543

Author(s):

Fan Yang ◽

Hang-Yi Pu ◽

Aftab Yaseen ◽

Bin Chen ◽

Fu Li ◽

...

Keyword(s):

Inflammatory Activity ◽

Aerial Parts ◽

Phenolic Derivatives ◽

Anti Inflammatory

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Phytochemical Screening, Biological Studiesand GC-MS Analysis of Extract of Biden pilosa L

Journal of Manmohan Memorial Institute of Health Sciences ◽

10.3126/jmmihs.v5i1.24076 ◽

2019 ◽

Vol 5 (1) ◽

pp. 79-93

Author(s):

Dharma Prasad Khanal ◽

Rupa Rana ◽

Bechan Raut ◽

Rabindra Prasad Dhakal

Keyword(s):

Ethyl Acetate ◽

Aerial Part ◽

Inflammatory Activity ◽

Alcoholic Extract ◽

Acute Oral Toxicity ◽

Oral Toxicity ◽

Bidens Pilosa ◽

Aerial Parts ◽

Ms Analysis ◽

Anti Inflammatory

Objective: The aim of the research work was to carry out the extraction of areal parts of Biden pilosa L by hydroalcholic and Hexane, ethyl acetate and acetone mixture followed by qualitative phytochemical analysis, acute oral toxicity test, anti-inflammatory test and GC-MS analysis of the extracts. Method: The hydro-alcoholic and HEA(n-hexane, ethyl acetate and acetone) extraction was done from aerial parts using ethanol and water in the ratio 70:30 and n-hexane, ethyl acetate and acetone in the ratio of 1:1:1 (HEA extract) respectively. Acute oral toxicity testwas performed OECD guidelines. The single spot in TLC was obtained using n-hexane as solvent for HEA fraction and finally phytocomponents were identified by GC-MS present in that spot.In vitro anti-inflammatory activity was performed by human RBC membrane stabilization method. Result: The phytochemical test results obtained indicate that hydro-alcoholic extract of aerial part of Bidens pilosa L. possess alkaloids, tannins, terpenoids and saponins whereas HEA extract possess alkaloids, flavonoids, tannins. Both hydro-alcoholic and HEA extracts were found to be safe up to the dose of 2000 mg/kg BW of the mice. Both extracts showed significant in vitro anti-inflammatory activity in a concentration dependent manner. The GC-MS analysis of HEA extract of aerial parts showed the presence of the sixteen different compounds from partially separated extract from TLC plates. Conclusion: Hydro-alcoholic extract of aerial part of Bidens pilosa L. possess alkaloids, tannins, terpenoids and saponins whereas HEA extract possess alkaloids, flavonoids, tannins. Both hydro-alcoholic and HEA extracts were found to be safe up to the dose of 2000 mg/kg BW of the mice. The GC-MS analysis of HEA extract of aerial parts showed the presence of the sixteen different compounds.

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In Vitro Interactions of Moroccan Propolis Phytochemical’s on Human Tumor Cell Lines and Anti-Inflammatory Properties

Biomolecules ◽

10.3390/biom9080315 ◽

2019 ◽

Vol 9 (8) ◽

pp. 315 ◽

Cited By ~ 3

Author(s):

Soraia I. Falcão ◽

Ricardo C. Calhelha ◽

Soumaya Touzani ◽

Badiaâ Lyoussi ◽

Isabel C. F. R. Ferreira ◽

...

Keyword(s):

Phenolic Compounds ◽

Tumor Cell ◽

Cell Lines ◽

Human Tumor ◽

Inflammatory Activity ◽

Tumor Cell Lines ◽

Human Tumor Cell ◽

Cell Lung Carcinoma ◽

Human Tumor Cell Lines ◽

Anti Inflammatory

Propolis is a resin manufactured by bees through the mixture of plant exudates and waxes with secreted substances from their metabolism, resulting in a complex mixture of natural substances of which quality depends on the phytogeographic and climatic conditions around the hive. The present study investigated the contribution of phenolic compounds to the cytotoxic and anti-inflammatory activities of propolis. The phenolic composition was evaluated by liquid chromatography with diode-array detection coupled to electrospray ionization tandem mass spectrometry (LC/DAD/ESI-MSn) analysis after phenolic extraction. The cytotoxicity of the extracts was checked using human tumor cell lines (MCF7- breast adenocarcinoma, NCI-H460- non-small cell lung carcinoma, HeLa- cervical carcinoma, HepG2- hepatocellular carcinoma, and MM127- malignant melanoma), as well as non-tumor cells (a porcine liver primary culture-PLP2). The anti-inflammatory activity was assessed using the murine macrophage (RAW 264.7) cell line. The results showed a composition rich in phenolic acids, such as caffeic and p-coumaric acid, as well as flavonoids, such as pinocembrin, pinobanksin, and pinobanksin-3-O-butyrate. Samples MP2 from Sefrou and MP3 from Moulay Yaâcoub presented a high concentration in phenolic compounds, while MP1 and MP4 from Boulemane and Immouzzer Mermoucha, respectively, showed similar composition with low bioactivity. The higher concentration of phenolic compound derivatives, which seems to be the most cytotoxic phenolic class, can explain the pronounced antitumor and anti-inflammatory activity observed for sample MP2.

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Health-Promoting Effects of Thymus Phenolic-Rich Extracts: Antioxidant, Anti-inflammatory and Antitumoral Properties

Antioxidants ◽

10.3390/antiox9090814 ◽

2020 ◽

Vol 9 (9) ◽

pp. 814

Author(s):

Andrea F. Afonso ◽

Olívia R. Pereira ◽

Susana M. Cardoso

Keyword(s):

Phenolic Compounds ◽

Mediterranean Region ◽

The West ◽

Numerous Species ◽

Anti Cancer ◽

Growing Body ◽

Potential Applications ◽

Health Promoting ◽

Salvianolic Acids ◽

Anti Inflammatory

Thymus genus comprises numerous species that are particularly abundant in the West Mediterranean region. A growing body of evidence suggests that many of these species are a rich source of bioactive compounds, including phenolic compounds such as rosmarinic acid, salvianolic acids and luteolin glycosides, able to render them potential applications in a range of industrial fields. This review collects the most relevant studies focused on the antioxidant, anti-inflammatory and anti-cancer of phenolic-rich extracts from Thymus plants, highlighting correlations made by the authors with respect to the main phenolic players in such activities.

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