Comparative efficiency of physiotherapeutic and medicinal treatment of microelements during reparative skin regeneration

2021 ◽  
pp. 120-126
Author(s):  
М.М. Шарипова
Keyword(s):  
Wound Healing ◽  
Comparative Study ◽  
Morphological Characteristics ◽  
Skin Wound ◽  
Skin Regeneration ◽  
Wound Surface ◽  
Herbal Preparations ◽  
Comparative Efficiency ◽  
Medicinal Treatment ◽  
Positive Effect

Проведено сравнительное изучение влияния двух богатых микроэлементами растительных препаратов и облучения лампами полого катода (ЛПК), содержащими спектры магния и меди - ЛПК Mn&Cu в экспериментальной модели репаративной регенерации кожи. Наносимая на поверхность раны смесь препаратов Метосепт и Витанорм и их пероральное введение в дозах 40-60 мг/крысу на 30% ускоряет репаративную регенерацию кожи за счёт увеличения количества дендритных клеток и макрофагов в очаге воспаления. Ежедневное 2-минутное облучение ЛПК Mn&Cu приводило к положительному изменению динамики морфологических характеристик процесса регенерации кожных ран, ускорению дифференцировки эпителиальных клеток и также к 30%-му ускорению заживления ран. Сравнительное изучение эффективности излучения пяти фототерапевтических аппаратов показало, что уменьшение площади раневой поверхности быстрее всего происходило под влиянием магнитолазерного воздействия аппаратом «Орион+», 2-е место по этому показателю разделили аппараты «Минитаг» и ЛПК Zn&Mn, 3-е - ЛПК Zn и «Биоптрон». A comparative study was conducted of the effect of herbal preparations and irradiation with hollow cathode lamps (HCL) producing magnesium and copper spectra, i.e., the Mg & Cu HCL, in an experimental model of reparative skin regeneration. A mixture of two micronutrient-rich drugs, Metosept and Vitanorm, were applied to the surface of the wound. Their oral administration in doses of 40-60 mg/rat accelerated the reparative skin regeneration by 30% by increasing the number of dendritic cells and macrophages in the focus of inflammation. Daily 2-min irradiation of the Mg & Cu HCL had a positive effect on the dynamics of the morphological characteristics of the skin wound regeneration process. There occurred an acceleration of epithelial cell differentiation, and also a 30% acceleration of wound healing. A comparative study of the radiation efficiency of five phototherapy devices showed that the wound surface reduction most quickly occurred during treatment with the magnetic laser “Orion+” apparatus. The 2nd most effective treatment was Minitag and HCL Zn & Mg, and the 3rd most effecivet was HCL Zn and Bioptron.

In vitro comparative study of human mesenchymal stromal cells from dermis and adipose tissue for application in skin wound healing

2019 ◽  
Vol 13 (5) ◽  
pp. 729-741 ◽  
Author(s):  
Helena Debiazi Zomer ◽  
Gisele Kristina dos Santos Varela ◽  
Priscilla Barros Delben ◽  
Diana Heck ◽  
Talita da Silva Jeremias ◽  
...  
Keyword(s):  
Wound Healing ◽  
Adipose Tissue ◽  
Comparative Study ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Human Mesenchymal Stromal Cells

Potential Beneficial Effects of Digitaria ciliaris Flower Absolute on the Wound Healing-Linked Activities of Fibroblasts and Keratinocytes

Planta Medica ◽  
2020 ◽  
Vol 86 (05) ◽  
pp. 348-355 ◽  
Author(s):  
Soo Min Park ◽  
Kyung Jong Won ◽  
Dae Il Hwang ◽  
Do Yoon Kim ◽  
Ha Bin Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Fibroblasts ◽  
Skin Wound ◽  
Skin Regeneration ◽  
Mitogen Activated Protein Kinase ◽  
Boyden Chamber ◽  
Type I ◽  
Skin Wound Healing ◽  
Beneficial Effects ◽  
Ms Analysis

Abstract Digitaria ciliaris is widely reported to be a problematic weed in agricultural areas and is mainly used as an indicator plant for the development of herbicides. However, its bioactivities on skin regeneration and wound healing have not been investigated. In the present study, we investigated the effects of D. ciliaris flower absolute on skin wound healing and skin regeneration-related events, that is, proliferation, migration, and collagen biosynthesis, in human fibroblasts and keratinocytes. For this study, we extracted absolute from the D. ciliaris flower by solvent extraction and identified the composition of D. ciliaris flower absolute using GC/MS analysis. We also tested the effect of D. ciliaris flower absolute in CCD986sk fibroblasts and/or HaCaT keratinocytes using the WST assay and 5-bromo-2′-deoxyuridine incorporation assay, Boyden chamber assay, ELISA, sprouting assay, and immunoblotting. GC/MS analysis of D. ciliaris flower absolute revealed that it contained 15 compounds. The absolute increased the proliferations of keratinocytes and fibroblasts and the migration of fibroblasts but did not affect cell viabilities. In addition, it enhanced the syntheses of type I and IV collagen in fibroblasts, but not in keratinocytes. The sprouting assay showed increased sprout outgrowth of fibroblasts. In addition, D. ciliaris flower absolute induced the phosphorylation of extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase in fibroblasts. These results indicate that D. ciliaris flower absolute may promote skin wound healing/regeneration by inducing the proliferation, migration, and collagen synthesis of fibroblasts, as well as the proliferation of keratinocytes. Therefore, D. ciliaris flower absolute may be a potential natural source for cosmetic or pharmaceutical agents that promote skin wound healing/regeneration.

scholarly journals Skin Wound Healing Process and New Emerging Technologies for Skin Wound Care and Regeneration

Pharmaceutics ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 735 ◽  
Author(s):  
Erika Maria Tottoli ◽  
Rossella Dorati ◽  
Ida Genta ◽  
Enrica Chiesa ◽  
Silvia Pisani ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Healing Process ◽  
Skin Wound ◽  
Skin Regeneration ◽  
Bioactive Molecules ◽  
Wound Healing Process ◽  
Skin Wound Healing

Skin wound healing shows an extraordinary cellular function mechanism, unique in nature and involving the interaction of several cells, growth factors and cytokines. Physiological wound healing restores tissue integrity, but in many cases the process is limited to wound repair. Ongoing studies aim to obtain more effective wound therapies with the intention of reducing inpatient costs, providing long-term relief and effective scar healing. The main goal of this comprehensive review is to focus on the progress in wound medication and how it has evolved over the years. The main complications related to the healing process and the clinical management of chronic wounds are described in the review. Moreover, advanced treatment strategies for skin regeneration and experimental techniques for cellular engineering and skin tissue engineering are addressed. Emerging skin regeneration techniques involving scaffolds activated with growth factors, bioactive molecules and genetically modified cells are exploited to overcome wound healing technology limitations and to implement personalized therapy design.

Wound Healing Microenvironmental Cues: From Tissue Analogs to Skin Regeneration

2013 ◽  
Vol 2 (2) ◽  
pp. 145-153 ◽  
Author(s):  
Mariana Cerqueira ◽  
Rui Reis ◽  
Alexendra Marques
Keyword(s):  
Wound Healing ◽  
Skin Regeneration

scholarly journals Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

2019 ◽  
Vol 20 (15) ◽  
pp. 3679 ◽  
Author(s):  
Lin Chen ◽  
Alyne Simões ◽  
Zujian Chen ◽  
Yan Zhao ◽  
Xinming Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Wound Closure ◽  
Expression Patterns ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Specific Expression ◽  
Keratinocyte Proliferation

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

scholarly journals A pulsatile release platform based on photo-induced imine-crosslinking hydrogel promotes scarless wound healing

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jian Zhang ◽  
Yongjun Zheng ◽  
Jimmy Lee ◽  
Jieyu Hua ◽  
Shilong Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Skin Wound ◽  
Large Animal ◽  
Skin Wounds ◽  
Tgfβ Signaling ◽  
Pulsatile Release ◽  
Ecm Extracellular Matrix

AbstractEffective healing of skin wounds is essential for our survival. Although skin has strong regenerative potential, dysfunctional and disfiguring scars can result from aberrant wound repair. Skin scarring involves excessive deposition and misalignment of ECM (extracellular matrix), increased cellularity, and chronic inflammation. Transforming growth factor-β (TGFβ) signaling exerts pleiotropic effects on wound healing by regulating cell proliferation, migration, ECM production, and the immune response. Although blocking TGFβ signaling can reduce tissue fibrosis and scarring, systemic inhibition of TGFβ can lead to significant side effects and inhibit wound re-epithelization. In this study, we develop a wound dressing material based on an integrated photo-crosslinking strategy and a microcapsule platform with pulsatile release of TGF-β inhibitor to achieve spatiotemporal specificity for skin wounds. The material enhances skin wound closure while effectively suppressing scar formation in murine skin wounds and large animal preclinical models. Our study presents a strategy for scarless wound repair.

scholarly journals The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 457
Author(s):  
Andreu Blanquer ◽  
Jana Musilkova ◽  
Elena Filova ◽  
Johanka Taborska ◽  
Eduard Brynda ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Chronic Wounds ◽  
Human Keratinocytes ◽  
Skin Wound ◽  
Platelet Lysate ◽  
Therapeutic Approaches ◽  
Skin Wound Healing ◽  
New Therapeutic Approaches ◽  
Proliferation And Differentiation

Chronic wounds affect millions of patients worldwide, and it is estimated that this number will increase steadily in the future due to population ageing. The research of new therapeutic approaches to wound healing includes the development of nanofibrous meshes and the use of platelet lysate (PL) to stimulate skin regeneration. This study considers a combination of a degradable electrospun nanofibrous blend of poly(L-lactide-co-ε-caprolactone) and poly(ε-caprolactone) (PLCL/PCL) membranes (NF) and fibrin loaded with various concentrations of PL aimed at the development of bioactive skin wound healing dressings. The cytocompatibility of the NF membranes, as well as the effect of PL, was evaluated in both monocultures and co-cultures of human keratinocytes and human endothelial cells. We determined that the keratinocytes were able to adhere on all the membranes, and their increased proliferation and differentiation was observed on the membranes that contained fibrin with at least 50% of PL (Fbg + PL) after 14 days. With respect to the co-culture experiments, the membranes with fibrin with 20% of PL were observed to enhance the metabolic activity of endothelial cells and their migration, and the proliferation and differentiation of keratinocytes. The results suggest that the newly developed NF combined with fibrin and PL, described in the study, provides a promising dressing for chronic wound healing purposes.

scholarly journals Sus Scrofa immune tissues as a new source of bioactive substances for skin wound healing

2020 ◽  
Author(s):  
Alexandr Basov ◽  
Liliya Fedulova ◽  
Ekaterina Vasilevskaya ◽  
Ekaterina Trofimova ◽  
Nataliya Murashova ◽  
...  
Keyword(s):  
Wound Healing ◽  
Sus Scrofa ◽  
Skin Wound ◽  
Bioactive Substances ◽  
Skin Wound Healing ◽  
Immune Tissues
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