scholarly journals The blood serum Na+/K+ ratio in orphan diseases

2021 ◽  
pp. 34-41
Author(s):  
Ю.В. Наточин ◽  
А.А. Кузнецова ◽  
А.В. Нистарова
Keyword(s):  
Blood Serum ◽  
Sharp Increase ◽  
Kinetic Method ◽  
Extracellular Fluid ◽  
Community Acquired Pneumonia ◽  
Control Group ◽  
Healthy Children ◽  
Lung Pathology ◽  
Orphan Diseases ◽  
K Concentration

Введение. Концентрация Na+ и K+ в клетке и внеклеточной жидкости обусловливает фундаментальные свойства организма. В работе использовано Na+/К+ отношение сыворотки крови как патофизиологический критерий состояния человека при сходной патологии легких, но генетически разных орфанных заболеваниях. Цель исследования - изучение соотношения концентрации ионов Na+ и K+ в сыворотке крови как интегрального патофизиологического параметра базовой функции клеток при орфанных заболеваниях сопряженных с патологией легких. Методика. Обследованы 99 детей, в том числе 50 с 3 формами орфанной патологии легких. Среди них 24 ребенка с муковисцидозом (МВ), 15 детей с первичным иммунодефицитом (ПИД), 11 - с первичной цилиарной дискинезией (ПЦД). Контрольную группу- составили15 здоровых детей, группу сравнения - 34 пациента с внебольничной пневмонией, в том числе 15 с тяжелым течением. Возраст обследованных от 3 мес до 17 лет. Концентрация ионов Na+, K+, Cl-, измерялась ионоселективными электродами на анализаторе Erba XL-200, креатинина - кинетическим методом по реакции Яффе без депротеинизации на анализаторе Erba XL-200. Результаты. Концентрация Na+ и К+ в сыворотке крови у пациентов всех групп соответствовала стандартам нормы, однако расчет Na+/К+ отношения выявил статистически значимые отличия от показателей контрольной группы (30.40 ± 0.48) у пациентов с МВ, ПИД, ПЦД и при внебольничной пневмонии средней степени тяжести - 32.7±0.8, 33.2±0.9, 32.83±0.8 и 33±1, соответственно, (р<0.05). Различий при разных вариантах орфанных болезней не выявлено. Резкое увеличение Na+/K+ отношения наблюдалось в остром периоде пневмонии с тяжелым течением 38.8±1.1 (р<0.001). Повышение Na+/K+ отношения обусловлено снижением концентрации K+ в сыворотке крови. Клиренс креатинина сохраняется в границах нормы, что свидетельствует о сохранности гомеостатической, ионорегулирующей функции почек. Заключение. Na+/K+ отношение в сыворотке крови повышено у пациентов с орфанными заболеваниями органов дыхания и при пневмонии у детей. Острое увеличение Na+/K+ отношения в сыворотке наступает при резком ухудшении состояния пациентов. Высказано предположение, что увеличение Na+/K+ отношения в сыворотке крови обусловлено изменением функционального состояния клеток, их объема. Introduction. Concentrations of Na+ and K+ in the cell and in the extracellular fluid determine fundamental properties of the organism. The study used the blood serum Na+/K+ ratio as a pathophysiological criterion for the state of patients with similar pathologies of the lungs but with genetically different orphan diseases. The aim of the study was to evaluate the Na+/K+ concentration ratio in the blood serum as an integral pathophysiological parameter of the basic cell function in orphan diseases associated with lung pathology. Methods. 99 children were examined, including 24 with cystic fibrosis (CF), 15 with primary immunodeficiency (PIDs), and 11 with primary ciliary dyskinesia (PCD). The control group consisted of 15 healthy children and the comparison group consisted of 34 patients with community-acquired pneumonia, including 15 with severe disease. The patients aged from 3 mos. to 17 years. Concentrations of Na+, K+, and Cl- were measured with ion-selective electrodes on an Erba XL-200 analyzer; creatinine was measured by the kinetic method using the Jaffe reaction without deproteinization on an Erba XL-200 analyzer. Results. Concentrations of Na+ and K+ in blood serum in all groups corresponded to normal, standard values. The Na+/K+ ratio was 30.4±0.48 in healthy people, 32.7±0.8, 33.2±0.9, 32.83±0.8, and 33±1 in patients with CF, PIDs, PCD, or community-acquired pneumonia of moderate severity, respectively (р<0.05). All these values were significantly higher than in the control group (p < 0.05). However, there were no differences for different orphan diseases. A sharp increase in the Na+/K+ ratio was revealed in the acute period of severe pneumonia (38.8±1.1, p < 0.001). The increase in Na+/K+ ratio was due to a decrease in serum K+ concentration. The creatinine clearance remained within the normal range, which indicated preservation of the homeostatic, ion-regulating function of the kidneys. Conclusion. The Na+/K+ ratio is increased in patients with orphan respiratory diseases and in children with pneumonia. A sharp increase in the Na+/K+ ratio is a manifestation of acute deterioration of the patient’s condition. Apparently, the increase in serum Na+/K+ ratio is caused by a change in cell volume.

scholarly journals Bactericidal capacity of oral neutrophils as a marker for clinical course of inflammatory ­respiratory diseases in children

2020 ◽  
Vol 101 (5) ◽  
pp. 740-748
Author(s):  
O I Pikuza ◽  
R A Fayzullina ◽  
A M Zakirova ◽  
Z Ya Suleymanova ◽  
E L Rashitova ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Respiratory Tract ◽  
Respiratory Diseases ◽  
Recovery Period ◽  
Neutrophil Migration ◽  
Community Acquired Pneumonia ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Children ◽  
Cationic Proteins

Aim. To study the number of neutrophils in the oral cavity, their bactericidal potential, to assess as an indicator for predicting the course of recurrent bronchitis (J40) and community-acquired focal pneumonia in children. Methods. 87 children between 5 and 10 years old, including 52 children with recurrent bronchitis and 35 with focal community-acquired pneumonia were observed. The control group consisted of 37 conditionally healthy children of a similar age. Viral antigens were studied by chemiluminescence immunoassay. Oral neutrophil counts and functional activity were determined. Antibacterial antibodies were measured by an enzyme-linked immunosorbent assay (ELISA). Results. 70.11% of patients had a viral antigen, and 57.47% had immunoglobulins M and G against bacterial pathogens. Oral neutrophil counts increased in the main group compared to the control group: up to 163.826.5 cells (p 0.001) in recurrent bronchitis, to 110.925.5 (p 0.05) in community-acquired pneumonia. By the recovery period, the number of oral neutrophils counts decreased in recurrent bronchitis (1.7 times higher compared to the control group, p 0.01) and remained practically unchanged in community-acquired pneumonia (115.026.9, p 0.05). Myeloperoxidase level had opposite changes for the groups compared to the control group: with recurrent bronchitis, it was 1.610.09 to the level in the control group (p 0.05), with community-acquired pneumonia 0.730.09 to the level in the control group (p 0.001). The level of lysosomal cationic proteins decreased to 0.770.09 to the level in the control group (p 0.05) in recurrent bronchitis, and to 0.800.09 (p 0.05) in pneumonia. Conclusion. In inflammation of the respiratory tract, neutrophil migration to the oral cavity, as well as myeloperoxidase level, increases, indicators of spontaneous luminol-dependent chemiluminescence are activated, and a deficiency of lysosomal cationic proteins occurs; this prevents the penetration of the pathogen into the lower respiratory tract.

DIAGNOSTIC SIGNIFICANCE OF BLOOD SERUM CYTOKINE PROFILE FOR CHRONIC KIDNEY DISEASE IN CHILDREN

2018 ◽  
Vol 22 (4) ◽  
pp. 81-89
Author(s):  
O. V. Semeshina ◽  
V. N. Luchaninova ◽  
A. Nee ◽  
E. V. Markelova ◽  
N. V. Gorelik ◽  
...  
Keyword(s):  
Blood Serum ◽  
Kidney Diseases ◽  
Prognostic Significance ◽  
Control Group ◽  
Specific Marker ◽  
Healthy Children ◽  
Serum Cytokine ◽  
Diagnostic Significance ◽  
Tnf Α ◽  
Tgf Β1

THE AIM: to study diagnostic and prognostic significance of blood serum cytokine status evaluation in children with different nosological forms of kidney diseases.PATIENTS AND METHODS. The study included 255 children with various kidney diseases (kidney stone disease (KSD) – 16, with  infectious kidney diseases (IKD) – 174, with a glomerulopathy (GP)  – 65). In all study groups were dominant children with 1st and 2nd  stage of CKD (100%, 97,5% and 95,4% respectively). The control group included 50 virtually healthy children. All patients  determined level of TNF-α, TNF-RI and TNF-RII, IL-10, TGF-β1 and TGF-β3, IL2, IL2-SR in blood serum.RESULTS.Increase of TNF-α level in blood serum can be considered as a highly specific marker of acute pielonephritis chronization, as  well as decrease of TNF-RII concentration can be considered as a  marker of full clinical and laboratory pielonephritis remission. The  increase in TNF-α and TNF-RI can also be considered as a marker of autoimmune inflammation. Deficiency of IL-2, IL-10 and TGF-β3  with an increase in IL-2 R in blood should be used as a marker of  bacterial-inflammatory and autoimmune kidney diseases, and TGF- β1 increase as an early marker of nephrosclerosis, especially in  patients with glomerulonephritis. The increase of the inflammatory  index TNF-α / IL-10 more than 4 times, gives us  the opportunity to  position it as an additional diagnostic criterion for infectious and  autoimmune process in the kidneys. The increase in urinary  excretion of TNF-α with the decrease of IL-10 by maintaining  consistently high concentrations of TGF-β1 is a marker of  inflammation and fibrosis in infectious kidney diseases and  glomerulonephritis. Modern nephroprotection therapy directed at  slowing progression of CKD and its complications should include  modulation of cytokine status.

scholarly journals Role of vitamin D, serum zinc, and serum iron deficiency in community-acquired pneumonia in children

2021 ◽  
Vol 69 (1) ◽  
Author(s):  
Asmaa Alhusseiny Ahmed Alsharkawy ◽  
Ahmed R. Rezk
Keyword(s):  
Vitamin D ◽  
Serum Iron ◽  
Serum Zinc ◽  
Community Acquired Pneumonia ◽  
Control Group ◽  
Iron Level ◽  
Healthy Children ◽  
Serum Iron Level ◽  
Vitamin D Levels ◽  
Significant Difference

Abstract Background Community-acquired pneumonia is a major cause of death among children. Inadequate nutrition disrupts the immune system and increases the susceptibility to infections. We aimed to evaluate the association between vitamin D, serum zinc, and iron, and pneumonia. A case-control study was conducted at the outpatient clinic and emergency room of Children’s Hospital. Thirty-one patients with community-acquired pneumonia and 36 healthy children (control group) underwent serum sampling for vitamin D, zinc, and iron. Results Most patients had mild form (n = 17, 54.8%). All patients survived and were discharged. The serum iron level showed a non-significant difference between pneumonia and control groups (p ˃ 0.05). TIBC, vitamin D, and serum zinc were significantly lower in the patient group than the control group (P value = 0.04, < 0.001, and 0.03, respectively). Vitamin D deficiency was highly associated with the severity of pneumonia (P value = 0.008). Conclusion Adequate serum zinc and vitamin D levels may be protective against infection with community-acquired pneumonia in children aged from 2 months to 5 years old, but not iron.

scholarly journals Influence of Nature of Infant Nutrition on Formation of Body Resistance

2021 ◽  
Vol 65 (6) ◽  
pp. 145-154
Author(s):  
T. G. Malanicheva ◽  
E. V. Agafonova ◽  
N. V. Ziatdinova ◽  
I. N. Skidan
Keyword(s):  
Incidence Rate ◽  
Respiratory Diseases ◽  
Comparison Group ◽  
Resistance Index ◽  
Community Acquired Pneumonia ◽  
Main Group ◽  
Control Group ◽  
Healthy Children ◽  
Acute Respiratory Diseases ◽  
Body Resistance

Purpose of the Study: Assessment of the influence of the type of the adapted infant milk formula in infants on the formation of the body resistance. Children Characteristics and Study Methods. 105 virtually healthy children were examined, as follows: 35 children were breastfed (control group), 70 children were formula fed including 35 children that received NENNY with prebiotics formula based on the goat milk (main group), 35 children – formulas based on the cow milk with probiotics and prebiotics (comparison group). The incidence rate of the acute respiratory diseases (ARD) as well as the number of the pneumonia histories in the infants were assessed, and the body resistance index was calculated. The laboratory assessment of the child body immunoresistance was carried out using the method of imprint smears from the nasal mucous membranes and the method of nasal washes. Results. It was determined that there were 2 times more children of the main group with no history of the acute respiratory diseases when infant than in the comparison group. In general, the good resistance (were sick 0-3 times a year) in the main group was registered in 82.9% of the children, while it was only 60% in the comparison group. The average number of the ARD cases per year was 2.3 ± 0.02 in the main group, which is 1.5 times less than in the comparison group – 3.6 ± 0.04 (p <0.05), and in 1, 3 times more than that in the control group – 1.8 + 0.03 (p<0.05). In average, the resistance index was 0.28 ± 0.02 in the main group, while it was 0.36 ± 0.03 in the comparison group, and 0.25 ± 0.02 in the control group. When studying the incidence rate of the community-acquired pneumonia in the infants, it was revealed that 2.8% of them were ill in the control group, 5.7% of them in the main group, and 8.5% in the comparison group. When feeding the child with formulas based on the goat’s milk, the level of mucosal immunity of the nasal mucosa and enzyme systems of the intraleukocyte microbicidal system is assured, which is virtually indistinguishable from the level of immunity achieved with breastfeeding. Conclusion. Adapted NENNY with prebiotics formulas can be recommended for children who are formula fed when infant in order to increase the body’s resistance and to reduce the incidence rate of the acute respiratory diseases and community-acquired pneumonia.

scholarly journals Endogenous Lactoferrin in the Assessment of Ferrokinetics in Children with Iron Deficiency States

2019 ◽  
pp. 44-50
Author(s):  
N. N. Klimkovich ◽  
G. P. Zubritskaya ◽  
E. I. Venskaya ◽  
A. G. Kutko ◽  
A. S. Skorobogatova ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Blood Serum ◽  
Biological Fluids ◽  
Laboratory Data ◽  
Deficiency Anemia ◽  
Control Group ◽  
Healthy Children ◽  
Physiological Mechanisms ◽  
Latent Iron Deficiency

Objective: to study the concentration of endogenous lactoferrin in children with iron deficiency states before and during the treatment. Material and methods . The clinical and laboratory data of 31 patients with iron deficiency states (24 patients with iron deficiency anemia and 7 with latent iron deficiency) aged 0-17 (the median age was 9.7) and 7 healthy children (the median age was 12.4) who made up the control group have been analyzed. Hemogram and biochemical blood test were done according to the standard method. The concentration of endogenous LF in the blood serum was determined by the method of enzyme immunoassay using commercial test systems for the quantitative determination of human LF in the biological fluids (Elabscience) on the universal VICTOR2TM analyzer (Perkin Elmer, USA). Results . The study of ferrokinetics and LF concentration in the blood serum of the children with iron deficiency states has been performed. It has been shown that the children with iron deficiency reveal an increased LF level in the blood serum, which has variable values depending on age. The highest levels of the serum LF content are characteristic of patients under the age of three months, which is associated with the physiological mechanisms of the adaptation process of the hematopoietic system in newborns and infants. The LF concentration in the blood serum associated with ferrotherapy is increased compared to the corresponding indices before the treatment. Conclusion . From the results of the study its follows that the content of LF in the blood serum increases in iron deficiency and is associated with ferrotherapy. The obtained results will be used in further research aimed at the diagnosis of iron deficiency and prediction of the response to iron therapy.

scholarly journals Comparative analysis of biochemical markers for rheumatic diseases of adults and children in normality and pathology

2019 ◽  
Vol 60 (11) ◽  
pp. 48-55
Author(s):  
Mikhail Y. Vlasov ◽  
◽  
Elena V. Pisareva ◽  
Yulia V. Golub ◽  
Pyotr P. Purygin ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Rheumatic Diseases ◽  
The Body ◽  
Joint Disease ◽  
Control Group ◽  
Hydroxyl Groups ◽  
Healthy Children ◽  
Hydroxyproline Content ◽  
Intercellular Substance

Collagen is an intercellular substance of connective tissue, it plays an important role in the physiological processes of the body. Collagen consists of three polypeptide chains twisted in a spiral and in order to stabilize this structure, hydroxyproline is needed, its hydroxyl groups are involved in the formation of hydrogen bonds between the chains of polypeptides. The peculiarity of collagen is that 14% of the amino acids it contains are hydroxyproline, an amino acid that is not contained in other proteins. In this regard, the content of hydroxyproline in blood serum was used to assess the metabolism of collagen in the body. We determined the content of hydroxyproline fractions in blood serum. Calcium, fibrinogen, erythrocyte settling rate and alkaline phosphatase level in children and adults with joint disease were also determined. The control group included healthy children and adults. The total hydroxyproline content in children with the disease was 9-11% lower than in healthy children. In adults with joint diseases, the total hydroxyproline content was 11% higher than in healthy patients. In children and adults with rheumatic diseases, the peptide-bound hydroxyproline content increased, while the protein-bound hydroxyproline content decreased in comparison with the control group control parameters. No significant changes in the content of calcium and fibrinogen were noted, but the level of alkaline phosphatase and the rate of erythrocyte sedimentation was increased, which is typical for people with inflammatory diseases.

scholarly journals Indicators of vitamin D in case of Coronavirus infection COVID-19 in children

2021 ◽  
pp. 34-39
Author(s):  
A.G. Hasanov ◽  
◽  
I.E. Huseinova ◽  
Keyword(s):  
Vitamin D ◽  
Blood Serum ◽  
Age Groups ◽  
Serum Vitamin ◽  
Main Group ◽  
Control Group ◽  
Healthy Children ◽  
Age Group ◽  
Subgroup Ii ◽  
Vitamin D Levels

Purpose — to determine and compare vitamin D levels in children diagnosed with COVID-19-associated pneumonia and healthy children. Materials and methods. The study involved 90 children. The main group consisted of 75 inpatient children with a diagnosis of COVID-19-associated pneumonia (positive PCR — polymerase chain reaction with reverse transcription). Patients in the main group were divided into 2 subgroups: subgroup I consisted of 49 patients with a pneumonia of moderate course, and subgroup II — 26 patients with a pneumonia of severe course. The control group consisted of 15 practically healthy children. The scheme of examination of sick children included anamnestic data, the use of clinical, instrumental and laboratory, immunological examination methods. The concentration of vitamin D in the blood serum of sick and healthy children was determined by the method of enzyme3linked immunosorbent assay (ELISA). The indicators were measured on a Stat Fax 4700 device using a Pishqaman reactive kit (made in Germany). Results. The concentration of vitamin D in blood serum was 1.8 times lower in subgroup I and 2.0 times in subgroup II, compared with the control group. The average values of vitamin D in blood serum compared with the control group were <1.1 times lower in the age group <1 year, 1.1 times lower in the age group 1–3 years, and 2.3 times lower in the age group >3 years. Conclusions. Based on our research, it can be concluded that there is a relationship between vitamin D deficiency and COVID-19. Thus, the average level of vitamin D was lower in children with COVID-19-associated pneumonia, and varied depending on the severity of the disease (more pronounced changes were observed in severe pneumonia). There are also differences in vitamin D levels in the age groups of children with COVID-19-associated pneumonia: in children from age groups under 1 year and 1–3 years old the decrease in serum vitamin D level is less pronounced than in the age group over 3 years old. The main mechanisms of vitamin D involvement in the immune modulation process in COVID-19 require further study. The research was carried out in accordance with the principles of the Helsinki declaration. The study protocol was approved by the Local ethics committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interest was declared by the authors. Key words: children, COVID-19, vitamin D.

scholarly journals AB0975 LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION IN CHILDREN WITH JUVENILE IDIOPATHIC ARTHRITIS ON BIOLOGICAL THERAPY

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1782.3-1782
Author(s):  
I. Chyzheuskaya ◽  
L. Byelyaeva ◽  
M. Kastsianevich ◽  
A. Vishnevskaya ◽  
I. Arher ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Juvenile Idiopathic Arthritis ◽  
Blood Serum ◽  
Optical Density ◽  
Biological Therapy ◽  
Joint Disease ◽  
Control Group ◽  
Central Research ◽  
Healthy Children ◽  
Antioxidant Protection

Background:Juvenile idiopathic arthritis (JIA) is a chronic immuno-inflammatory joint disease that leads to a child’s disability. Currently, drugs aimed at the main pro-inflammatory cytokines, such as tumor necrosis factor (TNF), interleukin-1 (IL-1), interleukin-6 (IL-6), and others, are successfully used to treat JIA. The effect of these drugs on metabolic processes has been little studied.Objectives:The purpose of the study was to determine the state of lipid peroxidation and antioxidant protection in children with JIA receiving biological therapy.Methods:28 children with polyarticular JIA, 15 children with systemic JIA and 20 healthy children were examined at the republican center of pediatric rheumatology on the basis of the rheumatology department of the 4th city children’s clinical hospital in Minsk. All patients received methotrexate, non-steroidal anti-inflammatory drugs, and glucocorticoids as needed.Determination of lipid peroxidation (LPO) and serum ACL and ACW were performed at the BelMAPO Central Research Laboratory. Statistical data processing was performed by traditional methods of variation statistics on a personal computer using the Statsoft Statistica 6.0 program.Results:During the study, prior to the use of tocilizumab, results were obtained that indicate the activation of lipid peroxidation processes and the violation of antioxidant defense processes in children with JIA. A significant (P <0.05) increase in the level of lipid peroxidation products in the blood serum of children with JIA compared with healthy children was established: the content of dienconjugates in the blood of children with JIA was 3.12±0.51 opt.pl., in healthy children - 1.65±0.4 units of opt.pl., the content of dienketones in children with JIA - 2.32±4839 units of opt.pl., in healthy children - 0.19±0.08 units of opt.pl., the content of malondialdehyde in children with JIA is 9.14±1.84 μmol/L, in healthy children - 7.13±1.35 μmol/L. A significant (P <0.01) decrease in the serum ACW and ACL in the blood serum of children with JIA was established when compared with the control group: the ACW content in children with JIA was 10.61±5.8 μmol/L, in healthy children - 13, 72±5.24 μmol/L, ACL content in children with JIA - 7.21±2.65 μmol/L, in healthy children - 8.81±3.5 μmol/L.During the treatment with tocilizumab, a remission of the disease was achieved. According to the results of a repeated study of lipid peroxidation and antioxidant protection 6 months after the start of biological therapy, a decrease in LPO activity and an increase in the antioxidant ability of substances in blood serum were found. Thus, the content of dienketones decreased to 1.05±0.17 units of optical density, dienconjugates to 2.4±0.6 units of optical density, and malondialdehyde to 6.3±1.7 μmol/l. The content of ACW increased to 12.91±3.3 μmol/L, and ACL to 8.9±3.5 μmol/L.Conclusion:The results indicate a positive effect of tocilizumab therapy on lipid peroxidation and antioxidant protection in children with JIA.Acknowledgments:This study would not have been possible without the collaboration of numerous Belarusian pediatric rheumatologists, patients and their parents.Disclosure of Interests:None declared

scholarly journals The role of matrix metalloproteinase-9 in the genesis of chronic bronchi inflammation in children with bronchial asthma

2020 ◽  
Vol 65 (2) ◽  
pp. 49-54
Author(s):  
A. A. Lebedenko ◽  
O. E. Semernik ◽  
M. V. Dudareva ◽  
E. B. Tyurina
Keyword(s):  
Blood Serum ◽  
Bronchial Asthma ◽  
Modern Medicine ◽  
Polymorphic Variant ◽  
Control Group ◽  
Healthy Children ◽  
Tract Disease ◽  
And Control

Bronchial asthma as the most common respiratory tract disease is an urgent problem of modern medicine. Matrix metalloproteinases (MMР) play a significant role in the processes of inflammation maintaining and bronchi remodeling in children with bronchial asthma.Objective: To study the role and inheritance of MMR9 in the bronchial asthma pathogenesis in children.Characteristics of children and research methods. The authors carried out the prospective study of 54 children with bronchial asthma and 56 healthy children with the determination of the MMP-9 level in blood serum, as well as the polymorphic variant 8202A>G of MMP9 gene.Results. Children with bronchial asthma had significantly higher content of MMP9 in the blood serum (431.76 [306.15; 612.93] ng/ml), as compared to the control group (276.05 [160.33; 397.02] ng/ml). When studying polymorphism 8202A>G of MMP9 gene in the examined patients it was found that the children with bronchial asthma had the G allele a little more often (57.5%) than A allele (42.5%). Heterozygous carriers of the MMP9 gene polymorphic variant predominate both among the patients with bronchial asthma and in the control group. When comparing the frequency of genotypes and alleles in the group of children with bronchial asthma and control group the authors revealed no statistically significant differences.Conclusion. The disease mechanism significantly depends upon the MMR9 concentration in the serum of patients with bronchial asthma and the peculiarities of this enzyme inheritance.

scholarly journals Concentration of 25(OH)D in blood serum and certain aspects of the clinical course of juvenile scleroderma

2021 ◽  
pp. 44-50
Author(s):  
L.I. Omelchenko ◽  
◽  
O.M. Mukvich ◽  
D.L. Ismakaeva ◽  
O.A. Belskaya ◽  
...  
Keyword(s):  
Vitamin D ◽  
Blood Serum ◽  
Clinical Course ◽  
Ethics Committee ◽  
Control Group ◽  
Healthy Children ◽  
Vitamin D Status ◽  
Vitamin D Supplements ◽  
Commercial Kits ◽  
Juvenile Scleroderma

Purpose — to assess the vitamin D supply in children and adolescents with systemic juvenile scleroderma (JS), taking into account its onset and clinical course at different periods of the child's development. Materials and methods. 14 children of 1 year 3 months —17 years old with systemic JS and 10 healthy children of the control group were examined. The concentration of 25(OH)D was determined in blood serum using commercial kits Vitamin D3 — Screeningkit, Switzerland, according to the manufacturer's instructions. Results. All patients with systemic JS showed a decrease in serum 25(OH)D levels — (24.55±9.32) ng/ml, compared to healthy children — (39.98±3.11) ng/ml. The lowest concentrations of the circulating form of vitamin D in the blood serum were in patients with limited form of systemic JS with Parry–Romberg hemiatrophy and «saber strike» — (14.07±3.38) ng/ml, as well as with the onset of generalized rapidly progressive JS in children at puberty — (16.31±4.6) ng/ml. Conclusions. Children with JS are shown to assess their vitamin D status by monitoring the serum concentration of 25(OH)D in order to decide whether to prescribe vitamin D supplements. The research was carried out in accordance with the principles of the Helsinki declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interest was declared by the authors. Key words: children, juvenile scleroderma, vitamin D.

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