scholarly journals Effect of garlic and ginger extracts on the levels of glucose and Peptide-c in diabetic mice: تأثير مستخلصي الثوم والزنجبيل في مستوى سكر الدم والببتيد c عند الفئران المستحدث عندها داء السكري

2021 ◽  
Vol 5 (4) ◽  
pp. 119-105
Author(s):  
Hiam Kamel Fadil, Kholoud Mostafa Sheikh Yousef Hiam Kamel Fadil, Kholoud Mostafa Sheikh Yousef
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Concentration ◽  
Physiological Saline ◽  
Alcoholic Extract ◽  
Diabetic Mice ◽  
Physiological Control ◽  
Fourth Group ◽  
The Third ◽  
Hypoglycemic Agent

This study aimed to investigate the effect of the alcoholic extract of garlic and ginger together on the levels of glucose, peptide -c and body weight in diabetic white mice. The study included 40 male white mice, Balb/c strain, which were divided into four experimental groups (10 mice in each group). The first group was a physiological control that was injected with physiological saline (0.9%) until the end of the experiment. As for the second group, diabetes was induced with a dose of 200 mg/kg of Alloxan hydrate weight of the mouse only, while the third group developed diabetes, and then it was treated with alcoholic extract of garlic and ginger together at a dose of 500 mg/kg of mouse weight for 10 days. While the fourth group developed diabetes and was treated with Glibenclamide. At the end of the experiment, the animals were anesthetized and blood was drawn from them by cardiocentesis. The results showed the effectiveness of garlic and ginger extracts in reducing blood glucose concentration by 35.75% and returning Peptide-c levels to their normal levels, equivalent to Glibenclamide (glyburide), which is known as an oral hypoglycemic agent.

scholarly journals In Vitro & In Vivo Anti-Hyperglycemic Potential of Saponins Cake and Argan Oil from Argania spinosa

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1078
Author(s):  
Rabie Kamal ◽  
Mourad Kharbach ◽  
Yvan Vander Heyden ◽  
Huiwen Yu ◽  
Abdelaziz Bouklouze ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Chemical Properties ◽  
Diabetic Mice ◽  
Argania Spinosa ◽  
Argan Oil

The Argan tree (Argania spinosa. L) is an evergreen tree endemic of southwestern Morocco. For centuries, various formulations have been used to treat several illnesses including diabetes. However, scientific results supporting these actions are needed. Hence, Argan fruit products (i.e., cake byproducts (saponins extract) and hand pressed Argan oil) were tested for their in-vitro anti-hyperglycemic activity, using α-glucosidase and α-amylase assays. The in-vivo anti-hyperglycemic activity was evaluated in a model of alloxan-induced diabetic mice. The diabetic animals were orally administered 100 mg/kg body weight of aqueous saponins cake extract and 3 mL/kg of Argan oil, respectively, to evaluate the anti-hyperglycemic effect. The blood glucose concentration and body weight of the experimental animals were monitored for 30 days. The chemical properties and composition of the Argan oil were assessed including acidity, peroxides, K232, K270, fatty acids, sterols, tocopherols, total polyphenols, and phenolic compounds. The saponins cake extract produced a significant reduction in blood glucose concentration in diabetic mice, which was better than the Argan oil. This decrease was equivalent to that detected in mice treated with metformin after 2–4 weeks. Moreover, the saponins cake extract showed a strong inhibitory action on α-amylase and α-glucosidase, which is also higher than that of Argan oil.

scholarly journals Effect of crude alcoholic celery (Apium graveolens) leaves extract on fertility in albino female mice

2011 ◽  
Vol 8 (1) ◽  
pp. 215-221
Author(s):  
Baghdad Science Journal
Keyword(s):  
Body Weight ◽  
Physiological Saline ◽  
Reproductive Organs ◽  
Apium Graveolens ◽  
Alcoholic Extract ◽  
Female Mice ◽  
The Third ◽  
Daily Dose ◽  
Positive Results ◽  
Albino Female

Sixty albino female mice were used in this experiment to study the possible effect of the crude alcoholic extract of the celery leaves on their fertility. These animals were randomly and equally divided into three experimental groups (20 females/group). The first and second groups were orally given a daily dose of 500 and 1000 mg/kg body weight, respectively, of the crude alcoholic extract, while the third group (control) was similarly treated, at the same time, with 0.1 ml/gm body weight of physiological saline for comparison. The treatment, however, lasted for 25 consecutive days. On day 26, after treatment was stopped, the life body weight of all animals was recorded before sacrificing the animals. Thereafter blood samples were collected by heart puncture for hormonal (estrogen, progesterone, luteinizing hormone and follicle stimulating hormone) determination. Reproductive organs (ovaries and uteri) were also isolated and preserved for histological studies. Active ingredients in the extracts were analytically tested. The test gave positive results for flavonoids, steroids, tannins and terbenoids. The effect of the extract on the studied parameters which are related to the fertility is reflected in the following results: 1) significant (p

TOTAL POLYPHENOL CONTENT, FREE RADICAL SCAVENGING, ALPHA-GLUCOSIDASE INHIBITION CAPACITY, THE EFFICACY IN CONTROLLING BLOOD GLUCOSE OF DIABETIC RATS OF THE MIXTURE VOS EXTRACTED FROM THE LEAVES OF CLEISTOCALYX OPERCULATUS, PSIDIUM GUAJAVA AND NELUMBO NUCIFERA

2013 ◽  
pp. 50-55
Author(s):  
Tuyet Mai Truong ◽  
Thi Lam Nguyen ◽  
Lan Anh Pham ◽  
Hoang Kien Truong
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Radical Scavenging ◽  
Psidium Guajava ◽  
Free Radical Scavenging ◽  
Diabetic Mice ◽  
Cleistocalyx Operculatus ◽  
Prevention And Treatment ◽  
Treatment Of Diabetes ◽  
Alpha Glucosidase

Objective: Plant polyphenols have antioxidant capacity and alpha-glucosidase inhibition to supporting for prevention and treatment of diabetes. Materials and Method: Present study was conducted to determine the content of total polyphenols, free radical scavenging and alpha-glucosidase inhibition of the VOS mixture that extracted from leaves (Voi leaves - Cleistocalyx operculatus (V), Oi leaves - Psidium guajava (O), Sen leaves - Nelumbo nucifera (S)). Results: The efficacy of blood glucose controlling in diabetic mice was investigated. After 8 weeks of administration with 200 mg VOS/kg body weight and 400 mg VOS/kg body weight, VOS diabetic mice had significantly reduced blood glucose level as compared to control diabetic mice. VOS diabetic mice with 400 mg dosage are lower in blood glucose levels than that of the diabetic mice with 200 mg. Also, the significant reducing in HbA1c was observed in VOS diabetic mice as compared with control diabetic mice. Conclusion: VOS-product extracted from Cleistocalyx operculatus leaves, guava leaves, lotus leaves might be considered as a safe product and to be a potential product in the supporting of prevention and treatment of diabetes.

Effect of Crude Leaves Extract of Bersama Abyssinica on Blood Glucose Level and Serum Lipid Level of Streptozotocin-Induced Diabetic Mice: Evidence for In vivo Antidiabetic Activity

2021 ◽  
Vol 19 ◽  
Author(s):  
Zemene Demelash Kifle ◽  
Agumas Alemu Alehegn ◽  
Baye Yrga Adugna ◽  
Abebe Basazn Mekuria ◽  
Engidaw Fentahun Enyew
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Serum Lipid ◽  
Lipid Level ◽  
Serum Lipid Level ◽  
Diabetic Mice ◽  
Lipid Levels ◽  
Antidiabetic Effect ◽  
Bersama Abyssinica

Background: Diabetes mellitus is one of the major and common metabolic, and chronic disorders in the world. Several medicinal plants have been used globally for the management of diabetes mellitus. The current study aimed to study the anti-hyperglycemic and anti-hyperlipidemic effects of Bersama abyssinica. Methods: Antidiabetic effect of 80% methanolic crude extract of Bersama abyssinica was studied in repeated dose-treated STZ-induced diabetic mice model. The activities of Bersama abyssinica on serum lipid level and body weight were investigated on STZ-induced diabetic mice. Data were analyzed using one-way ANOVA and significant when the p-value was less than 0.05. Results: All doses of the crude 80% methanolic extract of Bersama abyssinica (100 mg/kg, 200 mg/kg, and 400 mg/kg) exhibited a noticeable BGL reduction when compared with baseline blood glucose level and diabetic control on the 7th and 14th days of administration. Moreover, the higher dose of the extract (at 400 mg/kg) significantly (p < 0.001, 54.3%) decreased the BGL in STZ-induced diabetic mice. The maximum decrement in fasting BGL was achieved at the 14th days: 34.92%, 41.10%, 54.30%, and 59.66%, respectively for BAC 100 mg/kg, BAC 200 mg/kg, BAC 400 mg/kg, and GLC 5 mg/kg treated groups. Bersama abyssinica also displayed a significant (p < 0.05) improvement of serum lipid levels and body weight. Conclusion: Bersama abyssinica crude extract exhibited a significant antidiabetic effect and prevented body weight loss in streptozotocin-induced diabetic mice. The finding also confirmed the valuable biochemical activity of Bersama abyssinica by improving serum lipid levels.

scholarly journals d-Fagomine lowers postprandial blood glucose and modulates bacterial adhesion

2011 ◽  
Vol 107 (12) ◽  
pp. 1739-1746 ◽  
Author(s):  
Livia Gómez ◽  
Eunice Molinar-Toribio ◽  
María Ángeles Calvo-Torras ◽  
Carles Adelantado ◽  
M. Emília Juan ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Intestinal Mucosa ◽  
Bacterial Adhesion ◽  
Pathogenic Bacteria ◽  
Blood Glucose Concentration ◽  
Postprandial Blood Glucose ◽  
Dependent Manner ◽  
Food Component

d-Fagomine is an iminosugar originally isolated from seeds of buckwheat (Fagopyrum sculentumMoench), present in the human diet and now available as a pure crystalline product. We testedd-fagomine for activities connected to a reduction in the risk of developing insulin resistance, becoming overweight and suffering from an excess of potentially pathogenic bacteria. The activities were: intestinal sucrase inhibitionin vitro(rat mucosa and everted intestine sleeves), modulation of postprandial blood glucose in rats, bacterial agglutination and bacterial adhesion to pig intestinal mucosa. When ingested together with sucrose or starch,d-fagomine lowered blood glucose in a dose-dependent manner without stimulating insulin secretion.d-Fagomine reduced the area under the curve (0–120 min) by 20 % (P < 0·01) and shifted the time to maximum blood glucose concentration (Tmax) by 15 min at doses of 1–2 mg/kg body weight when administered together with 1 g sucrose/kg body weight. Moreover,d-fagomine (0·14 mm) agglutinated 60 % of Enterobacteriaceae (Escherichia coli,Salmonella entericaserovar Typhimurium) populations (P < 0·01), while it did not show this effect onBifidobacteriumspp. orLactobacillusspp. At the same concentration,d-fagomine significantly (P < 0·001) inhibited the adhesion of Enterobacteriaceae (95–99 % cells in the supernatant) and promoted the adhesion ofLactobacillus acidophilus(56 % cells in the supernatant) to intestinal mucosa.d-Fagomine did not show any effect on bacterial cell viability. Based on all this evidence,d-fagomine may be used as a dietary ingredient or functional food component to reduce the health risks associated with an excessive intake of fast-digestible carbohydrates, or an excess of potentially pathogenic bacteria.

scholarly journals Antihyperglycemic Activity of Hydroalcoholic Extracts of Selective Medicinal Plants Curcuma longa, Lavandula stoechas, Aegle marmelos, and Glycyrrhiza glabra and Their Polyherbal Preparation in Alloxan-Induced Diabetic Mice

Dose-Response ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 155932581985250 ◽  
Author(s):  
Sodah Bint Mustafa ◽  
Muhammad Akram ◽  
Hafiz Muhammad Asif ◽  
Imran Qayyum ◽  
Asif Mehmood Hashmi ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Therapeutic Potential ◽  
Curcuma Longa ◽  
Diabetic Mice ◽  
Aegle Marmelos ◽  
Antihyperglycemic Activity ◽  
Lavandula Stoechas

Background: Diabetes mellitus is a metabolic disorder associated with relative or absolute insulin deficiency or resistance, characterized by hyperglycemia. Modern prescriptions such as pioglitazone have better therapeutic potential, but its side effects and financial burden for developing countries have motivated the researchers to find alternative natural drugs to compete hyperglycemia in patients with diabetes. The present study was conducted to explore the therapeutic potential of selected medicinal plants for the treatment of diabetes as an alternative to allopathic medicines. Method: In present study, hydroalcoholic extracts of Curcuma longa, Lavandula stoechas, Aegle marmelos, and Glycyrrhiza glabra and their polyherbal preparation (PHP) as compound drug were investigated for their antihyperglycemic potential in alloxan-induced diabetic mice. The study subjects (mice) were divided into different groups as normal control, diabetic control, pioglitazone treated (standard drug), test groups (plant extract treated 50, 100, and 150 mg/kg body weight), and PHP-treated group. Blood glucose concentration of all the study animals was determined by Glucose strip test. Qualitative phytochemical analysis of all the plant extracts was also performed following standard methods. Result: It was investigated that treatment of alloxan-induced diabetic mice with hydroalcoholic extracts of studied medicinal plants showed significant ( P < .05) effects on fasting blood glucose levels (from baseline to normal range) in a manner comparable to that of the reference drug, pioglitazone (1 mg/kg body weight intraperitoneal). The tested plant extracts significantly ( P < .05) reduced the glucose concentration in blood of diabetes-induced mice in a dose-dependent manner. Conclusion: It could be concluded that studied medicinal plants have antihyperglycemic activity. The study findings favor the use of traditional herbal medicinal practices for the management of diabetes that might due to the presence of bioactive phytoconstituents in plants. However, larger studies are required to identify, isolate, and characterize the bioactive phytoconstituents responsible for antihyperglycemic activity of studied medicinal plants.

Astaxanthin enhances catalase activity in retinal tissue of experimental diabetic rats

2017 ◽  
Vol 2 (2) ◽  
pp. 147
Author(s):  
Shahnaz Mojarrab ◽  
Farideh Bahrami ◽  
Ali Khoshbaten ◽  
Mohammad Tagi Mohammadi ◽  
Fatemah Salem ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Concentration ◽  
Lethal Dose ◽  
Treated Group ◽  
Diabetic Rats ◽  
Fluid Retention ◽  
Diabetic Group ◽  
Retinal Ganglion ◽  
Retinal Tissue

It has been shown that diabetes causes a decrease in antioxidant capacity. Since the decrease of antioxidant capacities are related to the pathology of diabetic retinopathy; we aimed to evaluate the effect of Astaxanthin (ASTA), a powerful natural antioxidant, on catalase content in retinal tissue. Forty-eight rats were randomly divided into four groups (n =8-12 for each group): Control, Control-treated, Diabetic and Diabetic-treated groups. Diabetes was induced by a single injection of streptozotocin (50 mg/kg body weight, intravenously). Animals with a blood glucose more than 350 mg were selected as diabetic animals. Treatment with ASTA, (20 mg/kg) was administrated orally once a day by gavages over six weeks. After six weeks the final blood glucose concentration and body weight were measured. The animals were then euthanized by a lethal dose of Ketamine and Diazepam, the eyeballs removed and the retinas quickly extracted for catalase (CAT) measurement and histopathological assessments. Hyperglycemia decreased the enzymatic activity of CAT in retinal tissue of the Diabetic group but, with ASTA treatment, it returned toward normal value and ASTA treatment in Control-treated group increased the CAT capacity significantly. Retinal ganglion cell layer (RGC) in both Diabetic and Diabetic-treated groups showed fluid retention and edema but in the Diabetic-treated group, it was lower than inthe Diabetic group. We suggest that the increase of CAT activity and decrease of fluid retention in the Diabetic-treated group compared with the Diabetic group are related to the ability of ASTA to balance excess ROS production.

Blood eosinophilic reaction in young sheep at experimental dictyocaulosis and after the complex therapy

2016 ◽  
Vol 3 (3) ◽  
pp. 370-373
Author(s):  
Дегтяревская ◽  
T. Degtyarevskaya
Keyword(s):  
Body Weight ◽  
Standard Method ◽  
Moscow Region ◽  
Blood Eosinophil ◽  
Blood Samples ◽  
Fourth Group ◽  
Blood Eosinophilia ◽  
The Third ◽  
Complex Therapy

Objective of research: The study of eosinophilic reaction in blood at experimental dictycaulosis of young sheep and after providing the complex therapy. Materials and methods: The research was conducted in August- September 2013at an experimental farm «Kurilovo» in Podolsk district of Moscow region. Six mongrel lambs at 4–5 months of age, free from infection, and 18 lambs experimentally infected with Dictyocaulus filarial at gtye dose of 1000 larvae per head were investigated. 30 days after invasion, lambs were divided into 3 equal groups (6 head in each) and kept under conditions excluding the possibility of spontaneous invasion. Lambs of the first group free from infection, served as controls and did not receive the drug. Infected animas of the second group were not treated and served as controls. Lambs of the third group received Alben as 20% granulated powder at the dose of 5 mg a.i./kg (0,25 g of granules per 10 kg of body weight); the fourth group of lambs received Alben at the same dose and subcutaneously T-activin at the dose of 2 mkg/kg once a day on the 1st , 3rd and 7th day of invasion, and В-activin at the dose of 5 mkg/ kg intramusculary once a day during 5 days. Before the experiment and after 7, 15, 30 and 60 days of treatment, blood samples were taken from calves. Trilon B was used as anticoagulant. Blood eosinophil levels were measured by the standard method. Results and discussion: Persistent blood eosinophilia was determined at experimental dictycaulosis in lambs. The level of eosinophils cannot be fully restored by dehelmintization with Alben. The full restoration of the quantity of eosinophils in blood of infected lambs occurs after the complex therapy with Alben in combination with T and В-activin.

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