Blood eosinophilic reaction in young sheep at experimental dictyocaulosis and after the complex therapy

Objective of research: The study of eosinophilic reaction in blood at experimental dictycaulosis of young sheep and after providing the complex therapy. Materials and methods: The research was conducted in August- September 2013at an experimental farm «Kurilovo» in Podolsk district of Moscow region. Six mongrel lambs at 4–5 months of age, free from infection, and 18 lambs experimentally infected with Dictyocaulus filarial at gtye dose of 1000 larvae per head were investigated. 30 days after invasion, lambs were divided into 3 equal groups (6 head in each) and kept under conditions excluding the possibility of spontaneous invasion. Lambs of the first group free from infection, served as controls and did not receive the drug. Infected animas of the second group were not treated and served as controls. Lambs of the third group received Alben as 20% granulated powder at the dose of 5 mg a.i./kg (0,25 g of granules per 10 kg of body weight); the fourth group of lambs received Alben at the same dose and subcutaneously T-activin at the dose of 2 mkg/kg once a day on the 1st , 3rd and 7th day of invasion, and В-activin at the dose of 5 mkg/ kg intramusculary once a day during 5 days. Before the experiment and after 7, 15, 30 and 60 days of treatment, blood samples were taken from calves. Trilon B was used as anticoagulant. Blood eosinophil levels were measured by the standard method. Results and discussion: Persistent blood eosinophilia was determined at experimental dictycaulosis in lambs. The level of eosinophils cannot be fully restored by dehelmintization with Alben. The full restoration of the quantity of eosinophils in blood of infected lambs occurs after the complex therapy with Alben in combination with T and В-activin.

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Enhancement of corticosterone release by repeated injections of ACTH in the dexamethasone pre-treated rat

Acta Endocrinologica ◽

10.1530/acta.0.1020583 ◽

1983 ◽

Vol 102 (4) ◽

pp. 583-588 ◽

Cited By ~ 2

Author(s):

Noboru Murakami ◽

Kiyohisa Takahashi

Keyword(s):

Body Weight ◽

Adrenal Gland ◽

Adrenocorticotrophic Hormone ◽

Blood Samples ◽

Pulsatile Secretion ◽

The Third ◽

Daily Variations ◽

Corticosterone Response ◽

Corticosterone Release ◽

Adrenal Corticosterone

Abstract. To examine the possibility that pulsatile secretion of adrenocorticotrophic hormone (ACTH) enhances the responsiveness of the adrenal, the blood corticosterone response to repeated injections of ACTH was determined in the dexamethasone-nembutal pre-treated rat. Treatment with dexamethasone (100 μg/100 g body weight) at 13.00 h for 2 days decreased corticosterone levels and completely abolished these daily variations in both the blood and adrenal. Under these conditions, four or five successive iv injections of 0.2 or 2 mIU ACTH were given at ½ or 1 h intervals. Blood samples were taken immediately before and at frequent intervals after injection. A significant increase of blood corticosterone levels was observed 10 min after the second injection of 0.2 mIU ACTH. Further increases in blood corticosterone levels were observed after the subsequent injections. In addition, repeated injections of 2 mIU ACTH augmented the responsiveness of the adrenal to ACTH. The second or the third injections of 2 mIU ACTH produced a greater increase in adrenal corticosterone content than did the first ACTH injection. These results suggest that when ACTH acts on the adrenal gland in a pulsatile fashion, the steroidogenic response of the adrenal to ACTH increases markedly.

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Effect of garlic and ginger extracts on the levels of glucose and Peptide-c in diabetic mice: تأثير مستخلصي الثوم والزنجبيل في مستوى سكر الدم والببتيد c عند الفئران المستحدث عندها داء السكري

Journal of agricultural, environmental and veterinary sciences - مجلة العلوم الزراعية والبيئية والبيطرية ◽

10.26389/ajsrp.l010721 ◽

2021 ◽

Vol 5 (4) ◽

pp. 119-105

Author(s):

Hiam Kamel Fadil, Kholoud Mostafa Sheikh Yousef Hiam Kamel Fadil, Kholoud Mostafa Sheikh Yousef

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Blood Glucose Concentration ◽

Physiological Saline ◽

Alcoholic Extract ◽

Diabetic Mice ◽

Physiological Control ◽

Fourth Group ◽

The Third ◽

Hypoglycemic Agent

This study aimed to investigate the effect of the alcoholic extract of garlic and ginger together on the levels of glucose, peptide -c and body weight in diabetic white mice. The study included 40 male white mice, Balb/c strain, which were divided into four experimental groups (10 mice in each group). The first group was a physiological control that was injected with physiological saline (0.9%) until the end of the experiment. As for the second group, diabetes was induced with a dose of 200 mg/kg of Alloxan hydrate weight of the mouse only, while the third group developed diabetes, and then it was treated with alcoholic extract of garlic and ginger together at a dose of 500 mg/kg of mouse weight for 10 days. While the fourth group developed diabetes and was treated with Glibenclamide. At the end of the experiment, the animals were anesthetized and blood was drawn from them by cardiocentesis. The results showed the effectiveness of garlic and ginger extracts in reducing blood glucose concentration by 35.75% and returning Peptide-c levels to their normal levels, equivalent to Glibenclamide (glyburide), which is known as an oral hypoglycemic agent.

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Effect Of Vitamine AND Selenum On Immune Response Of CO - Baghdad Vaccine In Sheep.

The Iraqi Journal of Veterinary Medicine ◽

10.30539/ijvm.v45i1.1066 ◽

2004 ◽

Vol 45 (1) ◽

pp. 55-66

Author(s):

A. M.H. AL-Judi ◽

R. H. Hameed ◽

A. D. Salman

Keyword(s):

Immune Response ◽

Body Weight ◽

Vitamin E ◽

Antibody Titer ◽

Control Group ◽

Fourth Group ◽

The Third ◽

Significant Difference ◽

And Control

This study was done on 57 Awassi pregnant ewes, divided in to 5 groups, the 1st control group, the second group received E (900 mg) and Selenium (3mg), twicel.M.injections of vitamin the third group received one injection of vitamin E (900mg) and feed via selenium ( 3 mg), the fourth group received vitamin E block (1 gm) daily for two weeks, the fifth group received vitamin E via feed block for one week (1gm daily), and all groups injected with C Baghdad vaccine. This study had been shown a significant difference in antibody titer between treated groups and the control group, from2weeks post titre vaccination There were significant difference in antibody (p<0.05) between lambs of treated and control group. While there were no significant difference in body weight of ewes and lambs, production of treated and but was a significant difference in milk control group.

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Effect of Varying Doses of Estradiol Benzoate on the body weight and blood picture in Turkeys

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v36i2.460 ◽

2012 ◽

Vol 36 (2) ◽

pp. 174-179

Author(s):

Saad J. Gatie, Bassam A. Al-Shimmary

Keyword(s):

Body Weight ◽

Estradiol Benzoate ◽

Blood Parameters ◽

The Body ◽

Control Group ◽

Blood Picture ◽

Blood Samples ◽

The Third ◽

Control Body ◽

Rbc Count

A present study was carried out to investigate the effects of Estradiol benzoate 2 (mg/ml) hormone doses on the body weight and blood picture of Turkeys birds through studying the following parameters: body weight, RBC count , total WBC, MCV, MCH, MCHC, and platelet PLT. Forty growing Turkeys (30 days age) weighting between (250-350)gm were randomly divided into four groups (ten each group) treated as follows: Turkeys in the first group were injected with single dose of Estradiol benzoate (1 mg) on the first day of experiment (30 day post hatching) subcutaneously in the neck and termed as G1 group, the Turkeys in the 2nd group (G2) were injected Estradiol benzoate (2 mg) on the first day of experiment subcutaneously in the neck while the Turkeys in the third group (G3) were injected Estradiol benzoate (0.3 ml) at the first day of experiment subcutaneously in the neck the fourth group (G4) served as control, body weight was determined on (0, 15 and 30) days of experiment and blood samples were collected on 15th and 30th day of the experiment from groups to evaluate the blood parameters. Results revealed a significant increase (P<0.05) in body weight of G1, G2 and G3 in a comparison with control group at (15 and 30) days of experiment. Furthermore, in comparison among treated groups G2 and G3 also showed a significant increase (P<0.05) in the body weight as compared with G1 group during periods. Moreover, data revealed that all treated groups showed non-significant differences in blood parameters experiment as compared with control group.

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Study of some Impact Of Enterococcus faecium As Probiotic On Chick 1- Body Weight And Pathological Changes In Air Sacs And Intestine

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v31i1.806 ◽

2007 ◽

Vol 31 (1) ◽

pp. 70-77

Author(s):

Emad J. Khamas

Keyword(s):

Body Weight ◽

High Temperature ◽

Enterococcus Faecium ◽

Control Group ◽

Pathological Changes ◽

Fourth Group ◽

E Coli ◽

The Third ◽

Air Sacs ◽

The Impact

To investigate the impact of Enteococcus faecium as probiotic in layer chicks,this bacteria was given in feed at a dose rate of 1.5 gm per kg feed, each kg offeed contained 5×1011 cfu along the period of the study (57 days). 150 one dayold males were divided into five groups, 30 males each. The 1st group was giventhe probiotic only in the feed, the 2nd group was given the probiotic and exposedto high temperature and bad ventilation as a stress, and the third group wasgiven the probiotic with chlortetracycline in feed while the fourth group wasgiven the probiotic and then infected with E. coli on 21 day of age and the fifthgroup was given feed with out probiotic as the control group. The resultsshowed that there was increase in body weight especially in the last weeks of theexperiment. Turbidity of the air sacs was mild in the four group compared withcontrol. Intestinal villi became elongated due to using of probiotic and there wasless sloughing compared with control, and those groups showed resistancetoward E. coli infection.

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Role of ficus carica Leaves Extract in Treatment of Hypothyroidism

Journal of University of Babylon for Pure and Applied Sciences ◽

10.29196/jubpas.v26i10.1883 ◽

2018 ◽

Vol 26 (10) ◽

pp. 257-272

Author(s):

Abbas Abed Sharhan ◽

Kareem H. Rasheed

Keyword(s):

Body Weight ◽

Weight Gain ◽

Plant Extract ◽

Normal Saline ◽

Body Weight Gain ◽

Ficus Carica ◽

Fourth Group ◽

The Third ◽

Group I

This study was conducted to demonstrate the role of alcoholic extract from ficus carica leaves in the treatment of hypothyroidism disease which induced by carbimazole drug. Forty male rats were divided into five groups, eight for each . group I selected as negative control and administered orally with normal saline alone, group II served as positive control and treated by carbimazole anti-thyroid drug for six weeks , group III was treated by plant extract (500 mg /kg, bw) for six weeks, the fourth group IV was treated by carbimazole drug (5 mg) for six week to induce hypothyroidism and then treated by plant extract (500 mg /kg , bw) , and the fifth V group was treated by thyroxin drug (100 mg) for six week instead of plant extract. The results showed insignificant differences (P<0.05) in body weight gain in the fourth group ( carbimazole and plant extract) when compared with the first group (normal saline alone), second group (carbimazole alone), third group (plant extract alone , while the results demonstrated that a significant increase (P<0.05) in body weight gain when compared with fifth groups (carbimazole and thyroxine). While the results revealed insignificant differences (P<0.05) in the concentrations of T3 and T4 in the fourth (carbimazole & plant extract), fifth groups (carbimazole & thyroxin drug ) and the third group (plant extract alone) when compared with the first group ( normal saline alone ), but there was a significant increase (P<0.05) when compared with the second group (carbimazole alone) , in addition the results indicated a significant decrease (P<0.05) when compared with the third group, Furthermore, the results revealed insignificant difference (P<0.05) in TSH concentration in the fourth (carbimazole & plant extract), fifth groups (carbimazole & thyroxin drug) and the third group (plant extract alone) when compared with the first group (normal saline alone) , also the data showed a significant decrease(P<0.05) when compared with the second group (carbimazole alone). In conclusion, it is possible to use methanolic plant extract from ficus carica leaves in the regulation of hypothyroidism due to the presence of phytochemical components that can affect the mechanism of T3 and T4 production by the thyroid gland.

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Evaluation of the Human Pulmonary Activation-Regulated Chemokine (CCL18/PARC) and Alkaline Phosphatase (ALP) Levels in Iraqi Patients with Rheumatoid Arthritis

Iraqi Journal of Science ◽

10.24996/ijs.2020.61.4.1 ◽

2020 ◽

pp. 713-719

Author(s):

Nawal Haider Al-Hashimi ◽

Abdulnasser M. Al-Gebori ◽

Mohammed Hadi Munshed Alosami

Keyword(s):

Rheumatoid Arthritis ◽

Alkaline Phosphatase ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

Healthy Controls ◽

Blood Samples ◽

Fourth Group ◽

The Third ◽

Erythrocyte Sedimentation ◽

Age And Sex

Rheumatoid arthritis (RA) is one of the chronic inflammatory autoimmune diseases which occurs as a result of unknown reasons. This study was conducted at Baghdad Teaching Hospital/City of Medicine, where blood samples were taken from 60 Iraqi patients with RA (49 females and 11 males) and these patients were matched by age and sex with 20 healthy controls (16 females and 4 males). Patients with RA were diagnosed by a consultant rheumatologist according to ACR / EULAR criteria in 2010. In this study the patients were divided into four groups as follows; the first group consisted of 12 patients treated with methotrexate (MTX), the second group consisted of 10 patients treated with etanercept, the third group consisted of 18 patients treated with a combination of MTX, etanercept and prednisolone, the fourth group consisted of 20 patients treated with MTX and etanercept. Enzyme linked immunosorbent assay (ELISA) was used to detect CCL18/PARC antibodies, while a spectrophotometer (Humalyzer2000) was used for the measurement of alkaline phosphatase (ALP). Serum levels of CCL18 / PARC showed a significant increase in RA patients compared with healthy controls (p ≤ 0.001). The levels of CCL18/PARC showed a significant correlation with disease activity (CDAI), except in RA patients treated with etanercept. There was also no significant correlation between CCL18/PARC and erythrocyte sedimentation rate (ESR). The results showed a significant increase in serum levels of alkaline phosphatase (ALP) was recorded in RA patients (with treatment) as compared to healthy controls (p ≤ 0.001).

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Metabolic, gastrointestinal, and CNS neuropeptide effects of brain leptin administration in the rat

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1999.276.5.r1425 ◽

1999 ◽

Vol 276 (5) ◽

pp. R1425-R1433 ◽

Cited By ~ 4

Author(s):

Gertjan van Dijk ◽

Randy J. Seeley ◽

Todd E. Thiele ◽

Mark I. Friedman ◽

Hong Ji ◽

...

Keyword(s):

Fatty Acids ◽

Body Weight ◽

Energy Expenditure ◽

Neuropeptide Y ◽

Caloric Restriction ◽

Resting Energy Expenditure ◽

Corticotropin Releasing Hormone ◽

Plasma Fatty Acids ◽

The Third ◽

Ad Libitum

To investigate whether brain leptin involves neuropeptidergic pathways influencing ingestion, metabolism, and gastrointestinal functioning, leptin (3.5 μg) was infused daily into the third cerebral ventricular of rats for 3 days. To distinguish between direct leptin effects and those secondary to leptin-induced anorexia, we studied vehicle-infused rats with food available ad libitum and those that were pair-fed to leptin-treated animals. Although body weight was comparably reduced (−8%) and plasma glycerol was comparably increased (142 and 17%, respectively) in leptin-treated and pair-fed animals relative to controls, increases in plasma fatty acids and ketones were only detected (132 and 234%, respectively) in pair-fed rats. Resting energy expenditure (−15%) and gastrointestinal fill (−50%) were reduced by pair-feeding relative to the ad libitum group, but they were not reduced by leptin treatment. Relative to controls, leptin increased hypothalamic mRNA for corticotropin-releasing hormone (CRH; 61%) and for proopiomelanocortin (POMC; 31%) but did not reduce mRNA for neuropeptide Y. These results suggest that CNS leptin prevents metabolic/gastrointestinal responses to caloric restriction by activating hypothalamic CRH- and POMC-containing pathways and raise the possibility that these peripheral responses to CNS leptin administration contribute to leptin’s anorexigenic action.

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Immunity in human schistosomiasis mansoni: prevention by blocking antibodies of the expression of immunity in young children

Parasitology ◽

10.1017/s0031182000053956 ◽

1987 ◽

Vol 94 (2) ◽

pp. 281-300 ◽

Cited By ~ 98

Author(s):

A. E. Butterworth ◽

R. Bensted-Smith ◽

A. Capron ◽

M. Capron ◽

P. R. Dalton ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Young Children ◽

Immune Responses ◽

Blood Eosinophil ◽

Strongly Correlated ◽

Igg Antibodies ◽

Blood Samples ◽

Slow Development ◽

The Face ◽

Blocking Antibodies

SUMMARYA total of 129 children were treated forSchistosoma mansoniinfections, and followed for intensity of reinfection at3-monthly intervals over a 21-month period. Blood samples were taken before treatment and at 5 weeks and 6, 12 and 18 months after treatment. This paper presents a statistical analysis of the relationship between various immune responses and subsequent reinfection. Responses analysed were: blood eosinophil levels; IgE antibodies against schistosomulum antigens; IgG antibodies mediating eosinophil-dependent killing of schistosomula; antibodies inhibiting the binding to schistosomulum antigens of two rat monoclonal antibodies that also recognize egg antigens; the levels of anti-adult worm and of anti-egg (total, IgM and IgG) antibodies; and IgM anti-schistosomulum antibodies. Results for each assay were well correlated for each of the five separate blood samples. None of the assays were predictive of resistance to reinfection, butsusceptibilityto reinfection was strongly correlated with results in the preceding blood samples for total anti-egg antibodies and the inhibition of binding of one of the two monoclonal antibodies. Further analysis also revealed a correlation between reinfection intensities and both IgM anti-schistosomulum antibodies and IgM and IgG anti-egg antibodies. These results are consistent with the hypothesis that early infections elicit the development, in response to egg antigens, of antibodies that block immune mechanisms directed against schistosomula. Blocking antibodies may be IgM, but might also be of an ineffective IgG isotype. The existence of such antibodies in young children would explain the slow development of immunity in the face of a range of detectable, potentially protective immune responses.

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