scholarly journals Inferences of Carbon Dioxide in Present-Day Cell Culture Systems: An Unacknowledged Problem and Perspectives

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Dubey AK ◽  
◽  
Lavanya L ◽  
Sadananda D ◽  
Gouthami K ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Cell Culture ◽  
Culture System ◽  
Ph Regulation ◽  
Vital Role ◽  
Bicarbonate Buffer ◽  
Cellular Integrity ◽  
Carbon Dioxide Co2 ◽  
The Impact

The impact of cell culture technology has been immense in the field of research and pharmaceutical sciences. Suitable environmental conditions are paramount need in order to maintain the cell line in an in-vitro condition. Since the biological processes are highly susceptible to acid-base chemistry, variations among the factors will have a severe effect on cellular integrity that often leads to hypercapnia and senescence of the cells. pH regulation in the cell culture environment is a fundamental biological phenomenon of great significance for the growth and metabolism of cells. This review focuses on the implications of the two critical factors carbon dioxide (CO2), pH, and their correlated effects in the mammalian culture system. Bicarbonate buffer plays a vital role in maintaining homeostasis as dissolved CO2 hydration occurs in bicarbonate (HCO3-) and H+ equilibriums. If pH is not controlled, inhibition of CO2 causes acidification in the medium. In contrast, if pH is not regulated by integrating essential requirements, the equilibrium reaction shifts to the right towards the hydrogen ions that maintains the balance. Despite a few literatures exhibiting the role of carbon dioxide in cell culture, the present review distinguishes from them by showing the effects of CO2 in the in-vitro environment in maintaining the pH balance and cellular integrity. Thus, ensuring the proper mechanism of the utilization of CO2 and pH in the cell culture system will undoubtedly lead to the exploration of enormous concepts in the present crucial study.

scholarly journals Femtosecond pulsed laser microscopy: a new tool to assess the in vitro delivered dose of carbon nanotubes in cell culture experiments

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Dominique Lison ◽  
Saloua Ibouraadaten ◽  
Sybille van den Brule ◽  
Milica Todea ◽  
Adriana Vulpoi ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Cell Culture ◽  
Pulsed Laser ◽  
Lung Fibroblasts ◽  
Laser Microscopy ◽  
Cell Responses ◽  
The Impact ◽  
Delivered Dose ◽  
Femtosecond Pulsed Laser

Abstract Background In vitro models are widely used in nanotoxicology. In these assays, a careful documentation of the fraction of nanomaterials that reaches the cells, i.e. the in vitro delivered dose, is a critical element for the interpretation of the data. The in vitro delivered dose can be measured by quantifying the amount of material in contact with the cells, or can be estimated by applying particokinetic models. For carbon nanotubes (CNTs), the determination of the in vitro delivered dose is not evident because their quantification in biological matrices is difficult, and particokinetic models are not adapted to high aspect ratio materials. Here, we applied a rapid and direct approach, based on femtosecond pulsed laser microscopy (FPLM), to assess the in vitro delivered dose of multi-walled CNTs (MWCNTs). Methods and results We incubated mouse lung fibroblasts (MLg) and differentiated human monocytic cells (THP-1) in 96-well plates for 24 h with a set of different MWCNTs. The cytotoxic response to the MWCNTs was evaluated using the WST-1 assay in both cell lines, and the pro-inflammatory response was determined by measuring the release of IL-1β by THP-1 cells. Contrasting cell responses were observed across the MWCNTs. The sedimentation rate of the different MWCNTs was assessed by monitoring turbidity decay with time in cell culture medium. These turbidity measurements revealed some differences among the MWCNT samples which, however, did not parallel the contrasting cell responses. FPLM measurements in cell culture wells revealed that the in vitro delivered MWCNT dose did not parallel sedimentation data, and suggested that cultured cells contributed to set up the delivered dose. The FPLM data allowed, for each MWCNT sample, an adjustment of the measured cytotoxicity and IL-1β responses to the delivered doses. This adjusted in vitro activity led to another toxicity ranking of the MWCNT samples as compared to the unadjusted activities. In macrophages, this adjusted ranking was consistent with existing knowledge on the impact of surface MWCNT functionalization on cytotoxicity, and might better reflect the intrinsic activity of the MWCNT samples. Conclusion The present study further highlights the need to estimate the in vitro delivered dose in cell culture experiments with nanomaterials. The FPLM measurement of the in vitro delivered dose of MWCNTs can enrich experimental results, and may refine our understanding of their interactions with cells.

In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system

2020 ◽  
Vol 45 (5) ◽  
pp. 631-637
Author(s):  
Cansu Ozel-Tasci ◽  
Gozde Pilatin ◽  
Ozgur Edeer ◽  
Sukru Gulec
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Metabolic Diseases ◽  
Enzymatic Digestion ◽  
Cell Culture System ◽  
Culture Studies ◽  
Experimental Approaches ◽  
In Vitro Assessment

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.

Effect of ascorbic acid and citric acid on bioavailability of iron from Tegillarca granosa via an in vitro digestion/Caco-2 cell culture system

2021 ◽  
Author(s):  
Bolun Sun ◽  
Beibei Tan ◽  
Sun Nan ◽  
Ping Huang ◽  
Jingxia Hong ◽  
...  
Keyword(s):  
Cell Culture ◽  
Ascorbic Acid ◽  
Culture System ◽  
Iron Deficiency Anaemia ◽  
In Vitro Digestion ◽  
Cell Culture System ◽  
Iron Supplements ◽  
Deficiency Anaemia ◽  
Tegillarca Granosa

Iron deficiency anaemia (IDA) has been brought to worldwide attention. Developing safe and effective iron supplements is of great significance for IDA treatment. Tegillarca granosa (T. granosa), a traditional aquaculture...

Impact of structure, doping and defect- engineering in 2D materials for CO2 capture and conversion

2021 ◽  
Author(s):  
E. J. Jelmy ◽  
Nishanth Thomas ◽  
Dhanu Treasa Mathew ◽  
Jesna Louis ◽  
Nisha T Padmanabhan ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Co2 Capture ◽  
2D Materials ◽  
Vital Role ◽  
Energy Crisis ◽  
Two Dimensional ◽  
Defect Engineering ◽  
Anthropogenic Carbon ◽  
2D Nanomaterials ◽  
Carbon Dioxide Co2

The investigations on anthropogenic carbon dioxide (CO2) capture and conversion have a vital role in eradicating the global warming and energy crisis. In this context, defect- engineered two-dimensional (2D) nanomaterials...

Comparison between clinical response and in vitro drug sensitivity of primary human tumors in the adhesive tumor cell culture system.

1987 ◽  
Vol 5 (12) ◽  
pp. 1912-1921 ◽  
Author(s):  
J A Ajani ◽  
F L Baker ◽  
G Spitzer ◽  
A Kelly ◽  
W Brock ◽  
...  
Keyword(s):  
Cell Culture ◽  
Tumor Cell ◽  
Culture System ◽  
Distinct Advantage ◽  
Cell Culture System ◽  
True Value ◽  
Tumor Cell Culture ◽  
Prospective Trials ◽  
Tumor Types

The newly described adhesive tumor cell culture system (ATCCS) offers a distinct advantage over other assays in that it has a high plating efficiency requiring low cell inoculum, it affords workable assays in approximately 70% of specimens from the heterogenous tumor types, and it has the ability to assay up to nine drugs at four different concentrations. Clinical correlations based on the ATCCS were obtained in 65 patients undergoing 71 clinical trials. Patients with melanoma, lung cancer, and sarcoma dominated the group. The most active in vitro drug was correlated per clinical trial. Thirteen of 17 (76%) sensitive in vitro predictions and 51 of 54 (94%) resistant in vitro predictions were accurate. The assay in this study had a sensitivity of 81% and specificity of 93%. These preliminary results are encouraging and warrant prospective trials to establish the true value of this assay to patients.

SU-FF-J-104: In Vitro Model to Study the Biological Effect of Dose Gradients On a Cell Culture System

2005 ◽  
Vol 32 (6Part6) ◽  
pp. 1944-1944 ◽  
Author(s):  
R Bromley ◽  
L Oliver ◽  
R Harvie ◽  
R Davey
Keyword(s):  
Cell Culture ◽  
Biological Effect ◽  
Culture System ◽  
In Vitro Model ◽  
Cell Culture System ◽  
A Cell ◽  
Vitro Model

scholarly journals A Non-Cytotoxic Resin for Micro-Stereolithography for Cell Cultures of HUVECs

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 246 ◽  
Author(s):  
Max Männel ◽  
Carolin Fischer ◽  
Julian Thiele
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Umbilical Vein ◽  
Polymer Materials ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
3D Printed ◽  
Poly Ethylene ◽  
The Impact

Three-dimensional (3D) printing of microfluidic devices continuously replaces conventional fabrication methods. A versatile tool for achieving microscopic feature sizes and short process times is micro-stereolithography (µSL). However, common resins for µSL lack biocompatibility and are cytotoxic. This work focuses on developing new photo-curable resins as a basis for µSL fabrication of polymer materials and surfaces for cell culture. Different acrylate- and methacrylate-based compositions are screened for material characteristics including wettability, surface roughness, and swelling behavior. For further understanding, the impact of photo-absorber and photo-initiator on the cytotoxicity of 3D-printed substrates is studied. Cell culture experiments with human umbilical vein endothelial cells (HUVECs) in standard polystyrene vessels are compared to 3D-printed parts made from our library of homemade resins. Among these, after optimizing material composition and post-processing, we identify selected mixtures of poly(ethylene glycol) diacrylate (PEGDA) and poly(ethylene glycol) methyl ethyl methacrylate (PEGMEMA) as most suitable to allow for fabricating cell culture platforms that retain both the viability and proliferation of HUVECs. Next, our PEGDA/PEGMEMA resins will be further optimized regarding minimal feature size and cell adhesion to fabricate microscopic (microfluidic) cell culture platforms, e.g., for studying vascularization of HUVECs in vitro.

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