Effect of metformin gel against imiquimod–induced psoriasis in mice

Imiquimod-induced psoriasis is an animal model of psoriasis. The antidiabetic metformin had anti-inflammatory, immunomodulation, antiangiogenesis and antiproliferative effect. The objective of this study was to evaluate the possible beneficial effect of metformin gel against imiquimod-induced psoriasis-like form by scoring the erythema and scaling besides measuring of tissue homogenate levels of IL17/IL23. Swiss albino mice were used in this experiment imiquimod 5% cream applied on shaved back of mice for six days, and this is an induction group and compared with a negative control group that involved mice treated with ointment base only for six days. Third group; metformin 10% gel applied on the back of mice with imiquimod for six days. The fourth group included used of metformin 15% gel with imiquimod for six days. This data was analysed for significant level when p ≥ 0.05 by using either ANOVA test for biochemical parameters levels evaluation. Furthermore, imiquimod 5% cream of induction group caused an elevation of inflammatory parameters; IL17 and IL23 in skin tissue homogenate when being compared to IL17 and IL23 level of the control group. Whereas, metformin 10% gels showed levels of IL17 and IL23 with significant different from induction group. In addition, metformin 15% gels exerted a significantly low level of IL17 and IL23. The possible considerable antipsoriatic activity of topical metformin gel through reducing scaling and erythema and mediated through modest anti-inflammatory effects by suppressing levels of IL17/23.

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NEPHROPROTECTIVE EFFECT OF TUKHME KARAFS (APIUM GRAVEOLENS L.) AGAINST GENTAMICIN INDUCED NEPHROTOXICITY IN EXPERIMENTAL ANIMALS

INDIAN DRUGS ◽

10.53879/id.57.07.12276 ◽

2020 ◽

Vol 57 (07) ◽

pp. 63-69

Author(s):

Mohd Naushad ◽

Mohd Urooj ◽

Tasleem Ahmed ◽

Gulam Mohammed Husain ◽

Munawwar Husain Kazmi ◽

...

Keyword(s):

Biochemical Parameters ◽

Kidney Tissue ◽

Kidney Injury ◽

Negative Control Group ◽

Negative Control ◽

Apium Graveolens ◽

Tissue Homogenate ◽

Control Group ◽

Histopathological Study ◽

Nephroprotective Effect

Tukhme Karafs is the seed of Apium graveolens L., which is an important aromatic plant grown globally as a fresh herb and widely used as salad crop. This seed possesses potential pharmacological activity like hepatoprotective, diuretic and lithotriptic. The present study was designed to evaluate nephroprotective effect of Tukhme Karafs against gentamicin (80 mg/kg BW) induced nephrotoxicity in male Wistar rats. The effect was tested at two dose levels i.e. 500 mg/kg and 1000 mg/kg BW (calculated based on clinical dose mentioned in Unani literature). The study was divided into curative and protective models. The efficacy was determined based on estimation of biochemical parameters (creatinine, uric acid, BUN and serum electrolytes) along with quantification of kidney injury molecule-1 (KIM-1) using ELISA method in kidney tissue homogenate and histopathological study of kidney tissue. The findings of the study showed that Tukhme Karafs at both tested doses showed prominent reduction of biochemical parameters as compared to negative control group in both models. KIM-1 reduction was observed but was not statistically significant in both the models. Histopathological changes showed that drugs have inhibited gentamicin induced injury in both models. These results confirm the nephroprotective effect of Tukhme Karafs.

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ASSESSMENT OF ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF ASAM KANDIS (GARCINIA XANTHOCHYMUS HOOK. F. EX T. ANDERSON) FRUIT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i4.23578 ◽

2018 ◽

Vol 11 (4) ◽

pp. 81

Author(s):

Hanafis Sastra Winata ◽

Rosidah Rosidah ◽

Panal Sitorus

Keyword(s):

Ethanolic Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Inflammatory Activity ◽

Control Group ◽

Phytochemical Screening ◽

Fruit Extract ◽

Garcinia Xanthochymus ◽

Anti Inflammatory

Objective: The objective of this study was to evaluate the anti-inflammatory activity in acute and subacute models of inflammation from ethanolic fruit extract of Asam kandis (Garcinia xanthochymus Hook. f. ex T. Anderson) in animal (rats) models.Methods: Pleliminary phytochemical screening was carried out by using standard procedures.. Assessment of acute and subacute models of inflammation was using carrageenan-induced paw edema method and cotton pellet granuloma method using three dosage treatments; 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW along with a negative control group (0.5% Na CMC) and positive control (Na diclofenac 2.25 mg/kg BW). The inhibition period was observed at 30, 60, 90, 120, 150, and 180 min time intervals.Result: The phytochemical screening showed that the ethanolic fruit extract from Asam kandis contain contains flavonoids, glycosides, steroids, and triterpenoids. The anti-inflammatory result showed that the strongest inhibition produced by ethanolic fruit extract of Asam kandis occurred on the dosage of 800 mg/kg BW compared to the other doses (200 and 400 mg/kg BW) throughout the observation period.Conclusion: This finding indicated that ethanolic fruit extract of Asam kandis (G. xanthochymus Hook. f. ex T. Anderson) might become an interesting candidate for treatment of inflammation.

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Immunomodulatory and Anti-Inflammatory Potentials of Trigona Honey in the Therapy and Prevention against Respiratory Infection in Wistar Rats

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i3.2385 ◽

2020 ◽

Vol 11 (3) ◽

pp. 2955-2962

Author(s):

Ibrahim Khaled Al-kafaween ◽

Abu Bakar Mohd Hilmi ◽

Mohamed M. Soliman

Keyword(s):

Respiratory Infection ◽

Wistar Rats ◽

Serum Levels ◽

Negative Control Group ◽

Treated Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Gene Expressions ◽

Anti Inflammatory

Trigona honey (TH) is well known for its therapeutic characteristics. To date, the study of Trigona honey as a prophylactic or immune booster prior to the bacterial infection of the invivo model is not well covered. This study aims to investigate anti-inflammatory and immune activities in Wistar rats infected with respiratory infection following with Trigona honey. 25 Wistar rats were assigned to possitive groups, negative control group, positive control group was fed TH (5 g / kg body weight) orally, the untreated group was infected with Staphylococcus aureus to induce respiratory infection, the treated group has been infected with S. aureus followed by treatment with TH at a dose of 1.5 ×108 CFU / mL and the preventive group ingested TH one week before S. aureus infection. Blood was obtained for biochemical analysis. Lung tissues have been collected for molecular examination. The results showed a significant decrease in serum levels of ALT, AST, urea and creatinine in the preventive and treated groups, serum IgG increased significantly (P<0.05) in the preventive and treated groups, IFN-y increased in the preventive group while decreased in the treated group, and IL-8 increased in the treated group while decreased in the preventive group. The mRNA expression of AGP is up-regulated in the positive control, preventive and treated groups. The α2-MG, TNF-α , and mRNA expressions showed lower regulation after administration of TH in preventive and treated groups. The results show the ability of TH to counteract immune and inflammatory changes in serum levels and gene expressions.

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Regulation of Inflammatory Response in Human Osteoarthritic Chondrocytes by Novel Herbal Small Molecules

International Journal of Molecular Sciences ◽

10.3390/ijms20225745 ◽

2019 ◽

Vol 20 (22) ◽

pp. 5745 ◽

Cited By ~ 6

Author(s):

Ziadlou ◽

Barbero ◽

Stoddart ◽

Wirth ◽

Li ◽

...

Keyword(s):

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Catabolic Genes ◽

Osteoarthritic Chondrocytes ◽

Culture Model ◽

Tnf Α ◽

Anti Inflammatory ◽

Safranin O ◽

Vanilic Acid

In this study, 34 Traditional Chinese Medicine (TCM) compounds were screened for potential anabolic and anti-inflammatory properties on human osteoarthritic (OA) chondrocytes. The anabolic effects were assessed by measuring the glycosaminoglycan (GAG) relative to the DNA content using a 3D pellet culture model. The most chondrogenic compounds were tested in an inflammatory model consisting of 3 days of treatment with cytokines (IL-1β/TNF-α) with or without supplementation of TCM compounds. The anti-inflammatory effects were assessed transcriptionally, biochemically and histologically. From the 34 compounds, Vanilic acid (VA), Epimedin A (Epi A) and C (Epi C), 2′′-O-rhamnosylicariside II (2-O-rhs II), Icariin, Psoralidin (PS), Protocatechuicaldehyde (PCA), 4-Hydroxybenzoic acid (4-HBA) and 5-Hydroxymethylfurfural (5-HMF) showed the most profound anabolic effects. After induction of inflammation, pro-inflammatory and catabolic genes were upregulated, and GAG/DNA was decreased. VA, Epi C, PS, PCA, 4-HBA and 5-HMF exhibited anti-catabolic and anti-inflammatory effects and prevented the up-regulation of pro-inflammatory markers including metalloproteinases and cyclooxygenase 2. After two weeks of treatment with TCM compounds, the GAG/DNA ratio was restored compared with the negative control group. Immunohistochemistry and Safranin-O staining confirmed superior amounts of cartilaginous matrix in treated pellets. In conclusion, VA, Epi C, PS, PCA, 4-HBA and 5-HMF showed promising anabolic and anti-inflammatory effects.

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Evaluation of in-feed larch sawdust anti-inflammatory effect in sows

Polish Journal of Veterinary Sciences ◽

10.1515/pjvs-2017-0039 ◽

2017 ◽

Vol 20 (2) ◽

pp. 321-327

Author(s):

E.D. Tzika ◽

P.D. Tassis ◽

V.G. Papatsiros ◽

E.M. Pferschy-Wenzig ◽

A. Siochu ◽

...

Keyword(s):

Corn Starch ◽

Post Partum ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Lactation Period ◽

Basic Diet ◽

Anti Inflammatory ◽

Inflammatory Effect

Abstract The study aimed to investigate the possible anti-inflammatory activity of larch sawdust as feed supplement in lactating sows’ diet and its possible effect on the prevalence of Postpartum Dysgalactia Syndrome under field conditions. In a Greek farrow-to-finish pig farm, fifteen sows were randomly and equally allocated to a negative control group (NC group), a positive control group (PC group), and a treatment group (LT group). The animals of the first two groups received 99% basic diet and 1% corn starch, while LT group animals received 99% basic diet and 1% larch sawdust. The whole trial period lasted 35 days (7 days prior to farrow – day of weaning). At parturition day, animals of the PC group received 2 ml of an anti-inflammatory drug intramuscularly (meloxicam, Metacam®, Boehringer Ingelheim Vetmedica), while the animals of both other groups, received 2 ml of normal saline. Results showed insignificant differences among experimental groups for parameters such as post-partum rectal temperature and piglets performance. On the contrary, a significant increase of mean milk lactation index was observed in LT and PC groups on the 4th day of lactation period, when compared with NC group (p=0.014). Additionally, mean IL-6 concentrations in blood in the LT group showed a tendency for reduction when compared with those found in NC, and insignificant difference (p>0.05) when compared with those observed in PC group 24 hours postpartum. Moreover, the respective TNFα mean level in the LT group at 24 and 72 hours after parturition was similar to that found in PC group, respectively) and significantly lower than that determined in the NC group (p=0.003, p=0.024. The results suggest a possible anti-inflammatory effect of larch sawdust in sows.

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The immune response of rabbits immunized by Salmonella typhimurium and Lactobacillus acidophilus

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v42i1.27 ◽

2018 ◽

Vol 42 (1) ◽

pp. 28-34

Author(s):

Ikram A. A. Al-Samarraae

Keyword(s):

Salmonella Typhimurium ◽

Lactobacillus Acidophilus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Delayed Type Hypersensitivity ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

High Concentration ◽

Fourth Group

Antigens prepared from sonicated Salmonella typhimurium (KWCSA-ST) and Lactobacillus acidophilus (KWCSA-LBA) were used to evaluate synergistic effect on interlukine production (IL-2, IL-4) and Immunoglobulin-G (IgG) which were evaluated by using Enzyme Linked Immunosorbent Assay and Delayed Type Hypersensitivity-skin test at day 20 post immunization. Twenty five rabbits of both sexes, 2-3 Kg body weight, were divided into five equal groups; the first group immunized by KWCSA-ST (1000 µg/ml) and KWCSA-LBA (1000 µg/ml), the second group by KWCSA-ST (1000 µg/ml) and KWCSA-LBA (500 µg/ml), the third group by KWCSA-ST (1000 µg/ml) as positive control, the fourth group by KWCSA-LBA (1000 µg/ml) as positive control and the fifth group was injected by P.B.S. (pH7.2) as negative control group subcutaneously. The result of delayed type hypersensitivity showed an increase in the means of erythema and induration in the 1st group after 24hrs and 48hrs while the 4th group recorded the lowest mean after 24hrs and 48hrs. These means showed significant differences (P˂0.05) compared with injection by 1:2 and 1:4 diluted Ag. While the results of IgG showed that the highest concentration was at 35th day in the first group while the lowest concentration at 35th day in the fourth group with significant differences (P<0.05). Also the high concentration of IL-2, IL-4 was recorded in the first group at 35th day and the lowest concentration was in the fourth group at 35th day with significant differences (P<0.05); also the results showed significant differences (P<0.05) between the 1st, 2nd, 3rd compared with 4th and 5th groups.

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The Antidiabetic Effect of Low Doses ofMoringa oleiferaLam. Seeds on Streptozotocin Induced Diabetes and Diabetic Nephropathy in Male Rats

BioMed Research International ◽

10.1155/2015/381040 ◽

2015 ◽

Vol 2015 ◽

pp. 1-13 ◽

Cited By ~ 74

Author(s):

Abdulrahman L. Al-Malki ◽

Haddad A. El Rabey

Keyword(s):

Body Weight ◽

Diabetic Nephropathy ◽

Glycosylated Hemoglobin ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Tissue Homogenate ◽

Control Group ◽

Low Doses

The antidiabetic activity of two low doses ofMoringaseed powder (50 and 100 mg/kg body weight, in the diet) on streptozotocin (STZ) induced diabetes male rats was investigated. Forty rats were divided into four groups. The diabetic positive control (STZ treated) group showed increased lipid peroxide, increased IL-6, and decreased antioxidant enzyme in the serum and kidney tissue homogenate compared with that of the negative control group. Immunoglobulins (IgA, IgG), fasting blood sugar, and glycosylated hemoglobin(HbA1c)were also increased as a result of diabetes in G2 rats. Moreover albumin was decreased, and liver enzymes andα-amylase were not affected. In addition, the renal functions and potassium and sodium levels in G2 were increased as a sign of diabetic nephropathy. Urine analysis showed also glucosuria and increased potassium, sodium, creatinine, uric acid, and albumin levels. Kidney and pancreas tissues showed also pathological alteration compared to the negative control group. Treating the diabetic rats with 50 or 100 mgMoringaseeds powder/kg body weight in G3 and G4, respectively, ameliorated the levels of all these parameters approaching the negative control values and restored the normal histology of both kidney and pancreas compared with that of the diabetic positive control group.

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POTENTIAL ANTI-INFLAMMATORY ACTIVITY OF PLUMBAGO ZEYLANICA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i10.20357 ◽

2017 ◽

Vol 10 (10) ◽

pp. 372

Author(s):

Vetriselvan Subramaniyan ◽

Velmurugan Paramasivam

Keyword(s):

Test Dose ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Inflammatory Activity ◽

Control Group ◽

Paw Edema ◽

Plumbago Zeylanica ◽

Male Wistar Rats ◽

Anti Inflammatory

Objective: To determine the anti-inflammatory activity of dichloromethane extract of Plumbago zeylanica (DMEPZ), and its possible mechanism of action.Methods: Male Wistar rats (180-200 g) under controlled standard conditions (24±1°C, 55-58 humidity and 12 hrs light/dark cycle). The groups were divided into 5 groups (n=6/group) and assigned as positive control, negative control, and standard and two different test dose groups of P. zeylanica. Paw edema induced by subplantar injection of 0.1 mL of carrageenan (suspended in 1% carboxymethyl cellulose) into the right hind paw in all groups except negative control group. Granuloma induced by cotton pellets (10±1 mg) were implanted into groin region of each rat. The groups were divided into 4 groups (n=6/group) and assigned as possitive control, two different test dose groups of P. zeylanica and standard.Results: Oral administration of DMEPZ shown a significant (p<0.05) dose-dependent protection against carrageenan-induced paw edema. At 1st hr, P. zeylanica shown an inhibition effect of edema in the different doses of 250 mg/kg and 500 mg/kg were found to be 28.57 and 31.79%, respectively. At 3rd hrs, the paw edema inhibition was found to be 30.70 and 40.15%, respectively. Diclofenac (25 mg/kg) had effect of 34.10 and 41.73% (p<0.001) inhibition of paw edema at in 1 and 3 hrs. P. zeylanica 500 mg/kg showed percentage inhibition of wet and dry cotton pellet granuloma in rats 55.84% and 47.92%, respectively.Conclusion: Thus, the present study revealed that the DMEPZ offered significant protection against inflammation.

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The Effect of Ethanol Extract Turmeric Rhizome (Curcuma Longa) to Histologycal Structure of Rat Rectum Induced Dextran Sodium Sulphate (DSS)

Jurnal ILMU DASAR ◽

10.19184/jid.v20i1.7629 ◽

2019 ◽

Vol 20 (1) ◽

pp. 13

Author(s):

Lidia Maziyyatun Nikmah ◽

Susantin Fajariyah ◽

M. Mahriani

Keyword(s):

Goblet Cell ◽

Curcuma Longa ◽

Ethanol Extract ◽

Negative Control Group ◽

Negative Control ◽

Sodium Sulphate ◽

Control Group ◽

Dextran Sodium Sulphate ◽

Anti Inflammatory ◽

Group 1

Turmeric (Curcuma longa) is a plant that people use as a traditional medicine to treat some diseases such as: anti-inflammatory, antidiarrhea, antioxidants, hepatoprotector, and others. One of the main chemical compounds contained in turmeric and has a role as anti-inflammatory is curcumin. This study was conducted to determine the effect of ethanol extract of turmeric rhizome (Curcuma longa) on the histologycal structure of rat rectum induced by Dextran Sodium Sulphate (DSS). Wistar strain rats was divided into three groups: negative control group, positive control group (1% DSS), and DSS treatment group 1% + ethanol extract of turmeric rhizome dose 200mg/KgBB. DSS is given ad libitum, while ethanol extract of turmeric rhizome is given orally. We used paraffin method and Haematoxilyn Eosin staining to make the preparation organ. The data were analyzed using One Way ANOVA test, Duncan Multiple Range Test (DMRT) test, and correlation test. The result of this study is DSS administration has an effect on decreasing average of crypt, number of Goblet cell, and depletion of rectal mucous layer. While treatment of extract turmeric rhizome can increase height of crypt and number of Goblet cell in rectal mucosal layer of rat. Keywords: Exctract of turmeric rhizome, DSS, rectum, Rattus norvegicus

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AKTIVITAS ANTI-INFLAMASI EKSTRAK ETANOL TEPUNG PELEPAH AREN (Arenga pinnanta)

CHEMISTRY PROGRESS ◽

10.35799/cp.13.2.2020.31894 ◽

2020 ◽

Vol 13 (2) ◽

Author(s):

Devy Angreani M ◽

Meiske Sangi ◽

Feti Fatimah

Keyword(s):

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Inflammatory Activity ◽

Control Group ◽

Treatment Groups ◽

White Rats ◽

Wistar Strain ◽

Anti Inflammatory

Tepung pelepah aren secara tradisional digunakan sebagai obat untuk menghilangkan rasa gatal dan luka bakar pada kulit. Penelitian ini bertujuan untuk menguji aktivitas anti-inflamasi ekstrak etanol tepung pelepah aren (Arenga pinnanta) menggunakan metode induksi karagenan. Ekstrak etanol tepung pelepah aren yang digunakan didapatkan dari hasil maserasi tepung pelepah aren menggunakan etanol hasil redestilasi cap tikus. Metode pengujian aktivitas anti-inflamasi menggunakan 15 ekor tikus putih jantan galur wistar dengan berat 150-200 gram yang dibagi dalam 5 kelompok perlakuan, Kelompok Kontrol negative, Kontrol Positif dan kelompok dosis ekstrak etanol 20%, 25% dan 30%. Hasil penelitian ini menunjukkan kelompok ekstrak etanol tepung pelepah aren dosis 30% memiliki kemampuan inhibisi udem yang sama dengan kontrol positif (Na Diklofenak) dan memiliki kemampuan inhibisi yang lebih besar dibandingkan dosis 20% dan 25%.ABSTRACTPalm stem flour is traditionally used as a remedy for the relief of itching and burns on the skin. This research aims to test the anti-inflammatory activity of palm-flour (Arenga pinnanta) ethanol extract using Caragenan induction method. Palm-dried stem flour extract used from the maceration of palm flour, using ethanol redestilation of rat seals. The method of testing anti-inflammatory activity using 15 male white rats with Wistar strain with a weight of 150-200 grams divided into 5 treatment groups, negative control group, positive control and group dose of ethanol extract 20%, 25% and 30%. The results of this study showed that the group of dried palm flour extract dose 30% have the same inhibition capability with positive control (Na Diklofenak) and have greater inhibition capability compared to the dose 20% and 25%.

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