scholarly journals Immunodepressant and oxidant potential of standard leukaemia drug regimen

2020 ◽  
Vol 11 (4) ◽  
pp. 5608-5614
Author(s):  
Marwan M. Merkhan ◽  
Ibrahim M. Faisal ◽  
Dema Z. Alsaleem ◽  
Omer M. Shindala ◽  
Hani M. Almukhtar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Serum Levels ◽  
Drug Regimen ◽  
Significant Rise ◽  
Laboratory Method ◽  
Cytotoxic Drugs ◽  
Control Group ◽  
Healthy Individuals ◽  
Leukaemia Therapy ◽  
Immunoglobulin Levels

Chemotherapy improves cancer status but also could carry a risk of harmful side effects. In leukaemia patients, oxidative status and immune system are crucial parameters for patient improvement. The present study is aimed to assess the oxidative stress and the levels of immunoglobulin, including; IgG, IgA, and IgM in acute myelocytic leukaemias (AML) patients. A total of twenty-one patients with AML were enrolled in this study, alongside 24 healthy individuals as a control group. Initially, in both groups, the serum MDA, IgA, IgG, and IgM levels were measured. Then after a month of treatment with standard leukaemia therapy; the measurement of levels of these parameters were repeated for both the patients’ group and the control group for measuring immunoglobulin levels while analysis of MDA was done by the laboratory method. A highly significant rise in the serum MDA level was detected (P<0.001) in patients with AML after treatment with specific cytotoxic drugs was observed in comparison with the control. Moreover, there was a significant modulation in the serum concentration of Ig after starting cytotoxic drugs in comparison with the controls. The study revealed that AML was associated with elevated oxidative stress parameters following treatment with cytotoxic drugs as reflected by the rise in the serum levels of MDA. The decreased serum Ig levels indicate a reduction in immune response in patients with AML due to cytotoxic drugs.

scholarly journals Oxidative Stress Biomarkers as a Predictor of Stage Illness and Clinical Course of Schizophrenia

2021 ◽  
Vol 12 ◽  
Author(s):  
Dariusz Juchnowicz ◽  
Michał Dzikowski ◽  
Joanna Rog ◽  
Napoleon Waszkiewicz ◽  
Anna Zalewska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Regression Model ◽  
Antioxidant Status ◽  
Serum Levels ◽  
Stress Index ◽  
First Episode ◽  
Control Group ◽  
Healthy Individuals ◽  
Related Factors ◽  
Reducing Ability

Pro/antioxidant imbalance has been reported in schizophrenia (SZ). However, the results of studies are inconsistent and usually do not include other factors that are highly affected by oxidative stress (OS).This cross-sectional study aimed to determine the serum levels of OS markers and their potential connection with schizophrenia. The total sample comprised 147: 98 individuals with SZ −47 first-episode (FS) and 49 chronic patients (CS)—and 49 healthy individuals (HC) as a control group. The examination included clinical variables and serum levels of antioxidants and oxidative damage products. The significant changes were observed in concentrations of all examined markers, without any specific direction of the pro/antioxidant balance shift between SZ and HC. In the regression model adjusted for cofounders, catalase: OR = 0.81 (95%CI: 0.74–0.88); glutathione peroxidase: OR = 1.06 (95%CI: 1.02–1.10); total antioxidant capacity: OR = 0.85 (95%CI: 0.75–0.98); oxidative stress index: OR = 1.25 (95%CI: 1.03–1.52); ferric reducing ability of plasma: OR = 0.79 (95%CI: 0.69–0.89); advanced glycation end products: OR = 1.03 (95%CI: 1.01–1.04); and advanced oxidation protein products (AOPP): OR = 1.05 (95%CI: 1.03–1.07) turned out to be significant predictors of schizophrenia. In the multiple stepwise regression model, pro/antioxidant status and their interaction with the duration of illness-related factors affected schizophrenia symptoms: positive symptoms (FRAPxKYN), negative (DITYR, FRAP, CAT), general (KYN), and over-all psychopathology (KYNxNFK). The results confirm differences in serum levels of oxidative biomarkers between SZ patients and healthy individuals. The pro/antioxidant status could be considered a predictor of schizophrenia and the factor affects patients' symptom severity.

The Effect of Immunosuppression on Selected Antioxidant Parameters in Patients with Graves’ Disease with Active Thyroid-Associated Orbitopathy

2020 ◽  
Author(s):  
Magdalena Londzin-Olesik ◽  
Beata Kos-Kudla ◽  
Jacek Karpe ◽  
Aleksandra Nowak ◽  
Mariusz Nowak
Keyword(s):  
Oxidative Stress ◽  
Clinical Symptoms ◽  
Immunosuppressive Treatment ◽  
Serum Vitamin ◽  
Serum Levels ◽  
Paraoxonase 1 ◽  
Control Group ◽  
Graves Disease ◽  
Methylprednisolone Treatment ◽  
Antioxidant Parameters

Abstract Background and Study Aims Thyroid-associated orbitopathy, the most common extrathyroidal manifestation of Graves’ disease, is an autoimmune inflammation of orbital soft tissue. We report the study assessing the effect of immunosuppressive treatment with methylprednisolone on selected antioxidant parameters in patients with Graves’ disease with active thyroid-associated orbitopathy. Patients and Methods Activity and serum levels of selected antioxidant parameters as well as lipid peroxidation products were determined in a group of 56 patients with active thyroid-associated orbitopathy at three time-points: at baseline, after the discontinuation of intravenous methylprednisolone treatment and at 3 months after the discontinuation of additional oral methylprednisolone treatment. A control group consisted of 20 healthy age- and sex-matched volunteers. Results We found an increased activity of superoxide dismutase and glutathione peroxidase and increased serum levels of uric acid, malondialdehyde and conjugated dienes, as well as a reduced activity of paraoxonase-1 and reduced serum vitamin C level in the study group at baseline. Systemic intravenous and oral methylprednisolone therapy led to normalization of activity and concentration of the most studied parameters. Conclusion Results of our study confirmed that oxidative stress is one of the factors involved in the pathogenesis of thyroid-associated orbitopathy and the methyloprednisolone treatment is effective in reducing both clinical symptoms and oxidative stress in patients with this disease.

scholarly journals The Importance of Antioxidant Status in Gastric Intestinal Metaplasia

2021 ◽  
Author(s):  
Nilay Danis ◽  
Aysegul Ertinmaz Ozkan ◽  
Fatih Karatas ◽  
Cagri Cakici ◽  
Turkan Yigitbasi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Intestinal Metaplasia ◽  
Prognostic Marker ◽  
Smoking Status ◽  
Serum Levels ◽  
Control Group ◽  
Gastric Intestinal Metaplasia ◽  
Oxidative Stress Status ◽  
Cancer Types ◽  
The Mean

Background and Aim: Oxidative stress status in different cancer types was investigated before, but not studied in gastric intestinal metaplasia to the best of our knowledge. Purpose of this study is to examine whether there is a difference between oxidative stress status in patients with intestinal metaplasia (IM) compared to individuals without IM, we compared the serum levels of disulfide (SS), total thiol (TT) and native thiol (NT). Patients and Methods: This was a prospective, non-randomized case-control study including 67 patients with histopathologically confirmed IM and 60 individuals demographically matched in terms of age, gender, BMI, smoking status, and chronic diseases as control group. Results: The mean NT, TT and NT to TT(NT/TT) ratios were statistically significantly higher in IM group compared to controls [(351.71±81.9µmol/L vs. 271.82±54.13µmol/L, p=0.000),(391.5±92.69µmol/L vs. 308.59±55.53 µmol/L, 0.000) and (0.89±0.6 vs. 0.87±0.29,  p=0.022), respectively].The mean SS to TT(SS/TT) ratio was significantly lower in IM group than control group (0.050±0.31 vs. 0.060±0.014, P=0.022). Median SS and mean SS/NT ratio was similar in both groups[16.3 (3.3 – 78) vs. 18.3 (10 – 32.7), p=0.271 and 0.055 ± 0.041 vs. 0.070 ± 0.019, p=0.068, respectively). In ROC analysis, cut off value of SS/NT for IM was found 0.062, in regression analysis, SS/NT<0.062 was found as an independently prognostic marker for IM (OR, 2.38; 95%CI: 1.168 - 4.865, P=0.017). Conclusion: SS /NT ratio lower than 0.062 was found as an independently prognostic marker for IM. This ratio could help to distinguish which patients should be followed closely for gastric cancer.

scholarly journals Effects of l-Carnitine on the Follicle-Stimulating Hormone, Luteinizing Hormone, Testosterone, and Testicular Tissue Oxidative Stress Levels in Streptozotocin-Induced Diabetic Rats

2018 ◽  
Vol 23 ◽  
pp. 2515690X1879605 ◽  
Author(s):  
Nourollah Rezaei ◽  
Tahereh Mardanshahi ◽  
Majid Malekzadeh Shafaroudi ◽  
Saeed Abedian ◽  
Hamid Mohammadi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Group Vi ◽  
Group I ◽  
Stimulating Hormone

The present study was designed to investigate the antioxidant property of l-carnitine (LC) on serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (TH) and testis oxidative stress in streptozotocin (STZ)-induced diabetic rats. The rats were divided into the following groups: group I, control; group II, LC 100 mg/kg/d; group III, diabetic; and groups IV to VI, diabetic rats treated with 50, 100, and 200 mg/kg/d of LC, respectively. Daily injections were given intraperitoneally for 7 weeks. At the end of experimental period, after sacrificing the rats, FSH, LH, TH, total antioxidant capacity (TAC), malondialdehyde (MDA), glutathione (GSH), catalase (CAT), mitochondrial function (MTT), protein carbonyl (PC), and reactive oxygen species (ROS) levels were measured. STZ caused an elevation of MDA, ROS, and PC ( P < .001) with reduction of GSH, CAT, TAC, and MTT ( P < .001) in the serum levels. Group VI had significantly increased FSH, LH, and TH levels versus the untreated diabetic group ( P < .001). Although groups V and VI significantly decreased MDA ( P < .001), PC ( P < .01), and ROS ( P < .01) compared with the untreated diabetic group; only in group VI, the activity of GSH ( P < .001), CAT ( P < .01), TAC ( P < .001), and MTT ( P < .001) significantly increased. The results of the present study suggest that LC decreased diabetes-induced oxidative stress complications and also improved serum level of FSH, LH, and TH by reducing levels of lipid peroxidation and increasing antioxidant enzymes.

scholarly journals Hepatic Histomorphological Changes and Oxidative Stress Profile of Clarias gariepinus (Burchell, 1822) Juveniles Fed with Sodium Propanoate-Preserved Diets

2018 ◽  
Vol 10 (4) ◽  
pp. 460-465
Author(s):  
Patrick E. ABA ◽  
Ifeanyi E. UZOCHUKWU ◽  
Nelson I. OSSAI ◽  
Ifeanyi G. EKE
Keyword(s):  
Oxidative Stress ◽  
Biological Effects ◽  
Clarias Gariepinus ◽  
Basal Diet ◽  
Serum Levels ◽  
Control Group ◽  
Stress Profile ◽  
Fish Feed ◽  
Inflammatory Parameters ◽  
Significant Difference

Sodium propanoate is in the list of approved feed preservatives. However, there is dearth of information on its biological effects on the C. gariepinus. The present study investigated the effect of sodium propanoate-preserved feed on the hepatic histomorphometric changes, oxidative stress and inflammatory parameters of C. gariepinus juveniles. One hundred juveniles of mixed sexes, assigned into 5 groups of 20 fish per group, with each group consisting of 2 replicates of 10 fish, were used for the investigation. Group A juveniles were fed basal diet, while groups B-E received basal diet incorporated with sodium propanoate at the rate of 25, 50, 75 and 100 g/15 kg of feed respectively. Treatments were done two times daily for 8 weeks. Samples (sera and liver) were collected on the last day for evaluation of a few biochemical parameters (malondialdehyde values, catalase activity, C-reactive protein levels) and histomorphometric alterations in the liver. Results indicated that fish in groups D and E had higher catalase activities, lower serum levels of C-reactive proteins and an intact hepatic histomorphormetry when compared with the control group. There was no significant difference in the plasma malondialdehyde values in all the groups. It was concluded that preservation of fish feed with sodium propanoates improved antioxidant status of C. gariepinus and protected liver histology.

scholarly journals Lipoprotein-Associated Phospholipase A2 Is Increased in Patients with Impaired Bone Density

2014 ◽  
Vol 33 (4) ◽  
pp. 317-322
Author(s):  
Karel Kotaška ◽  
Jitka Kolárová ◽  
Blanka Jedličková ◽  
Jana Čepová ◽  
Jan Kotaška ◽  
...  
Keyword(s):  
Bone Density ◽  
Bone Resorption ◽  
Bone Metabolism ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Levels ◽  
Phospholipase A ◽  
Control Group ◽  
Low Density ◽  
Healthy Individuals

Summary Background: Increased levels of lipoprotein-associated phospholipase A2 are associated with atherosclerosis, and may contribute to cardiac disease. The aim of this study was to analyze serum levels of lipoprotein phospholipase A2 (Lp-PLA2) in patients with impaired bone resorption and correlate the findings with markers of bone metabolism (osteocalcin) and other biochemical markers (cholesterol, low density lipoprotein, triacylglycerols). Methods: Serum Lp-PLA2 was measured by a turbidimetric method in a group of currently treated 85 patients with impaired bone resorption and in a control group of 46 healthy individuals. Serum triacylglycerols was measured by the electrochemiluminescence immunoassay. Cholesterol, low density lipoprotein and triacylglycerols were measured by commercially available enzymatic assays. Bone density was investigated by dual energy X-ray densitometry performed on the lower spine and hips. Results: Concentrations of LP-PLA2 were significantly elevated in the patients with bone resorption compared to the control group of healthy individuals (225 ng/mL vs. 192 ng/mL, p>0.001) with the highest difference in patients with a T score below –2.5 SD (227 vs. 192 ng/mL). Serum levels of Lp-PLA2 also negatively correlated with decreased levels of serum osteocalcin in patients, and a significant difference in Lp-PLA2 (p=0.02) levels was observed between the control group and group with low levels of osteocalcin. Elevated Lp-PLA2 levels were significantly associated with the therapeutic procedures used, but not with age, gender and concentration of lipids. Conclusions: Lipoprotein-associated phospholipase A2 seems to play an important role also in bone metabolism.

scholarly journals Modulation of Cytokine and Cytokine Receptor/Antagonist by Treatment with Doxycycline and Tetracycline in Patients with Dengue Fever

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
J. E. Z. Castro ◽  
I. Vado-Solis ◽  
C. Perez-Osorio ◽  
T. M. Fredeking
Keyword(s):  
Supportive Care ◽  
Receptor Antagonist ◽  
Dengue Fever ◽  
Hemorrhagic Fever ◽  
Serum Levels ◽  
Significant Rise ◽  
Cytokine Receptor ◽  
Control Group ◽  
Moderate Increase ◽  
Cytokine Levels

Dengue virus infection can lead to dengue fever (DF) or dengue hemorrhagic fever (DHF). Disease severity has been linked to an increase in various cytokine levels. In this study, we evaluated the effectiveness of doxycycline and tetracycline to modulate serum levels of IL-6, IL-1B, and TNF and cytokine receptor/receptor antagonist TNF-R1 and IL-1RA in patients with DF or DHF. Hospitalized patients were randomized to receive standard supportive care or supportive care combined with doxycycline or tetracycline therapy. Serum cytokine and cytokine receptor/antagonist levels were determined at the onset of therapy and after 3 and 7 days. Cytokine and cytokine receptor/antagonist levels were substantially elevated at day 0. IL-6, IL-1β, and TNF remained at or above day 0 levels throughout the study period in untreated patients. Treatment with tetracycline or doxycycline resulted in a significant decline in cytokine levels. Similarly, IL-1RA and TNF-R1 serum concentrations were elevated at baseline and showed a moderate increase among untreated patients. Both drugs resulted in a significant rise in IL-1Ra levels by day 3 in patients. In contrast, treatment did not affect a similar result for TNF-R1. When compared to the control group, however, a significant rise post-treatment was seen upon intragroup analysis. Further analysis demonstrated that doxycycline was significantly more effective at modulating cytokine and cytokine receptor/antagonist levels than tetracycline.

scholarly journals Role of oxidative stress in hearing impairment in patients with type two diabetes mellitus

2009 ◽  
Vol 123 (9) ◽  
pp. 957-963 ◽  
Author(s):  
İ Aladag ◽  
A Eyibilen ◽  
M Güven ◽  
Ö Atış ◽  
Ü Erkokmaz
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Hearing Loss ◽  
Hearing Impairment ◽  
Protein Oxidation ◽  
Serum Levels ◽  
Control Group ◽  
Diabetic Patients ◽  
Enzymatic Antioxidants ◽  
Insulin Dependent Diabetic

AbstractObjective:Although many clinical investigations have found a relationship between hearing loss and diabetes mellitus, the pathophysiology of this effect remains controversial. To date, the mechanisms of hearing loss in diabetic patients have been explained in terms of microangiopathy, neuropathy and encephalopathy. However, many reports indicate that some diabetic complications are associated with oxidative stress related to the diabetes itself. In the present study, we hypothesised that oxidative stress may be a cause of hearing loss in diabetic patients.Methods:The study group comprised non-insulin dependent diabetic patients with no signs of microangiopathy or peripheral neuropathy. The control group comprised sex-, age- and body weight matched, non-diabetic subjects. Auditory function was evaluated using pure tone audiometry and tympanometry. Subjects with normal hearing and sensorineural hearing loss were included in the study, whereas subjects with conductive hearing loss were excluded. Both the study group (n = 63) and the control group (n = 37) were divided into subgroups based on the presence and absence of hearing loss. Oxidative stress was evaluated by measuring serum indicators of protein oxidation and lipid peroxidation, serum levels of nitric oxide and various non-enzymatic antioxidants, and the activity of various enzymatic antioxidants.Results:The non-insulin dependent diabetic patients had significantly higher serum levels of protein oxidation products, nitric oxide, enzymatic antioxidant activity (i.e. glutathione peroxidase and superoxide dismutase), compared with the control group (p < 0.05). When we compared the groups in relation to the presence of hearing loss, the nitric oxide level was significantly increased in the diabetic group with good hearing, compared with diabetic patients with hearing loss (p = 0.014). In the diabetic group, a clear, negative correlation was observed between serum levels of nitric oxide and vitamins C and E, and hearing impairment (r = −0.395,r = −0.318,r = −0.500, respectively). There was also a positive correlation between serum vitamin C concentrations and hearing levels in the control group (r = 0.417).Conclusion:These results suggest that oxidative stress may play an important role in hearing impairment in diabetic patients. In this process, increased protein oxidation appears to be more important than lipid peroxidation. Nitric oxide may have a protective effect on hearing, as may some nonenzymatic antioxidants such as vitamin C and E.

scholarly journals Prognosis of progression of chronic generalised periodontitis in patients with bronchiectatic disease

2020 ◽  
Vol 27 (1) ◽  
pp. 72-84
Author(s):  
Artem K. Sarkisov ◽  
Vladimir A. Zelenskiy ◽  
Ekaterina A. Polunina ◽  
Karen A. Sarkisov
Keyword(s):  
Oxidative Stress ◽  
Mathematical Model ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Binary Logistic Regression ◽  
Control Group ◽  
Healthy Individuals ◽  
Logistic Regression Method ◽  
High Level ◽  
And Oxidative Stress

Aim. To analyse the parameters of dental indices and the level of markers of infl ammation and oxidative stress in patients with chronic generalised periodontitis (CGP) with bronchiectatic disease and to develop a mathematical model for assessing the risk of CGP progression in patients with bronchiectatic disease.Materials and methods. A total of 70 patients with mild and moderate CGP were examined, which were divided into the following groups: patients with CGP without general somatic pathology (n = 33), and patients with CGP and bronchiectatic disease (n = 37). The control group consisted of somatically healthy individuals with intact periodontium (n = 40). Dental indices (PMA, PI, Muhlemann, OHI-s), infl ammatory markers (transforming growth factor beta 1 (TGFβ-1), lactoferrin (LF), interleukin-8 (IL-8), C — reactive protein (CRP)), and oxidative stress markers (malondialdehyde (MDA), as well as advanced oxidation protein products (AOPPs), and total superoxide dismutase (SOD)) were analysed in all patients included in the study. The method of binary logical regression was used to create a mathematical model for assessing the risk of CGP progression in patients with bronchiectatic disease.Results. Dental indices and the level of markers of infl ammation and oxidative stress were statistically signifi cantly higher in all patients with CGP as compared to somatically healthy individuals, as well as in patients with CGP and bronchiectatic disease as compared to patients with CGP without general somatic pathology. Positive correlations of different strength between the studied markers of infl ammation and oxidative stress and dental indices were revealed. Based on the results of the correlation matrix data and using the binary logistic regression method, a mathematical model was developed that can be applied for assessing the risk of CGP progression in patients with bronchiectatic disease. The predictors of progression included in the mathematical model were: PI, TGFβ-1 and AOPPs. Conclusion. The data obtained indicate a greater severity of infl ammation and oxidative stress in CGP patients with comorbid pathology in the form of bronchiectatic disease and the infl uence of these processes on the periodontal condition. The proposed mathematical model for assessing the risk of CGP progression in patients with bronchiectatic disease is characterized by a high level of sensitivity and prognostic signifi cance, thus being applicable for use in clinical practice. 

scholarly journals Puerarin mitigates acute liver injury in septic rats by regulating proinflammatory factors and oxidative stress levels

2021 ◽  
Vol 20 (11) ◽  
pp. 2305-2310
Author(s):  
Jinan Zheng ◽  
Qing Huang ◽  
Jingjing Fang
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Acute Liver Injury ◽  
Serum Levels ◽  
Expression Level ◽  
Sepsis Group ◽  
Control Group ◽  
Sprague Dawley ◽  
Tnf Α ◽  
Proinflammatory Factors

Purpose: To determine the protective effect of puerarin against acute liver injury in septic rats, and the mechanism involved.Methods: Eighty-seven Sprague-Dawley (SD) rats were assigned to control, sepsis and puerarin groups (each having 29 rats). Serum levels of NF-kB, TNF-α, IL-1 β, IL-6, ALT and AST were assayed. Liver lesions and levels of NO, SOD, iNOS and malondialdehyde (MDA) were measured using standard procedures.Results: Compared with the control group, the levels of NF-kB, TNF-α, IL-1β, IL-6, AST, ALT, NO, MDA and iNOS significantly increased in the sepsis group, while SOD level decreased significantly. In contrast, there were marked decreases in NF-kB, TNF-α, IL-1β, AST, ALT, NO, MDA and iNOS in puerarin group, relative to the sepsis group, while SOD expression level was significantly increased (p <0.05). The level of p-p38 in liver of septic rats was up-regulated, relative to control rats, while Nrf2 significantly decreased (p < 0.05). The expression level of p-p38 in the puerarin group was significantly decreased, relative to the sepsis group, while the expression level of Nrf2 significantly increased (p < 0.05).Conclusion: Puerarin mitigates acute liver injury in septic rats by inhibiting NF-kB and p38 signaling pathway, down-regulating proinflammatory factors, and suppressing oxidative stress. Thus, puerarin may be developed for use in the treatment liver injury.

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