scholarly journals Modulation of Cytokine and Cytokine Receptor/Antagonist by Treatment with Doxycycline and Tetracycline in Patients with Dengue Fever

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
J. E. Z. Castro ◽  
I. Vado-Solis ◽  
C. Perez-Osorio ◽  
T. M. Fredeking
Keyword(s):  
Supportive Care ◽  
Receptor Antagonist ◽  
Dengue Fever ◽  
Hemorrhagic Fever ◽  
Serum Levels ◽  
Significant Rise ◽  
Cytokine Receptor ◽  
Control Group ◽  
Moderate Increase ◽  
Cytokine Levels

Dengue virus infection can lead to dengue fever (DF) or dengue hemorrhagic fever (DHF). Disease severity has been linked to an increase in various cytokine levels. In this study, we evaluated the effectiveness of doxycycline and tetracycline to modulate serum levels of IL-6, IL-1B, and TNF and cytokine receptor/receptor antagonist TNF-R1 and IL-1RA in patients with DF or DHF. Hospitalized patients were randomized to receive standard supportive care or supportive care combined with doxycycline or tetracycline therapy. Serum cytokine and cytokine receptor/antagonist levels were determined at the onset of therapy and after 3 and 7 days. Cytokine and cytokine receptor/antagonist levels were substantially elevated at day 0. IL-6, IL-1β, and TNF remained at or above day 0 levels throughout the study period in untreated patients. Treatment with tetracycline or doxycycline resulted in a significant decline in cytokine levels. Similarly, IL-1RA and TNF-R1 serum concentrations were elevated at baseline and showed a moderate increase among untreated patients. Both drugs resulted in a significant rise in IL-1Ra levels by day 3 in patients. In contrast, treatment did not affect a similar result for TNF-R1. When compared to the control group, however, a significant rise post-treatment was seen upon intragroup analysis. Further analysis demonstrated that doxycycline was significantly more effective at modulating cytokine and cytokine receptor/antagonist levels than tetracycline.

PENGARUH PELATIHAN PENGENDALIAN VEKTOR DEMAM BERDARAH TERHADAP MAYA INDEX PADA SISWA SEKOLAH DASAR DI MAJALENGKA

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Wawan Kurniawan
Keyword(s):  
Vector Control ◽  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Intervention Group ◽  
Hemorrhagic Fever ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Quasi Experimental ◽  
Dengue Vector Control

ABSTRAK  Latar belakang: Demam Berdarah Dengue (DBD) disebabkan oleh virus Dengue dapat menyebabkan kematian. Pencegahan DBD yang dianggap paling tepat adalah Pemberantasan Sarang Nyamuk (PSN). Penelitian ini bertujuan untuk mengetahui pengaruh pelatihan pengendalian vektor Demam Berdarah pada siswa sekolah dasar terhadap Maya Index di Majalengka. Metode: Penelitian ini menggunakan desain quasi experiment (pretest-posttest control group design). Sebanyak 4 sekolah terpilih sebagai kelompok intervensi dan 4 sekolah lainnya sebagai kontrol. Subyek penelitian adalah siswa kelas IV-VI yang terdiri dari 171 siswa pada kelompok intervensi dan 163 pada kelompok kontrol. Instrumen yang digunakan adalah formulir pemantauan jentik berkala. Hasil: Jumlah rumah dengan kategori Maya Index tinggi berkurang dari 27,5% menjadi 9,4%. Terjadi penurunan angka BRI kategori tinggi pada kelompok intervensi dari 20,5% menjadi 1,8%. Pada kelompok kontrol tidak terjadi penurunan angka BRI kategori tinggi (22,1%), sebaliknya terjadi penurunan kategori rendah dari 34,4% menjadi 3,7%. Tidak terjadi penurunan angka HRI pada kelompok intervensi maupun kontrol. Kesimpulan: Pelatihan pengendalian vektor Demam Berdarah dapat menurunkan nilai BRI dan Maya Index, tetapi tidak berpengaruh terhadap nilai HRI. Tidak adanya perubahan nilai HRI menunjukkan bahwa kebersihan dan sanitasi lingkungan merupakan faktor yang berpengaruh terhadap probabilitas kejadian demam berdarah. Kata Kunci : Demam Berdarah, Maya Index, pelatihan, pengendalian vektor   ABSTRACT Background: Dengue Hemorrhagic Fever (DHF) caused by Dengue virus could cause death. The most appropriate prevention of Dengue is eradication of mosquito nests (PSN). This study aims to determine the effect of Dengue vector control training on elementary students towards Maya Index in Majalengka. Method: This study used quasi-experimental design (pretest-posttest control group design). A total of 4 schools were selected as intervention groups and 4 other schools as controls. The subjects were students in grades IV-VI consisting of 171 students in the intervention group and 163 in the control group. The instrument used was periodic larva monitoring form. Results: The number of houses with a high Maya Index category in the intervention group decreased from 27.5% to 9.4%. There was a decrease in the high BRI category in the intervention group from 20.5% to 1.8%. In the control group, there was no decrease in the high BRI category (22.1%), on the contrary, there was a decrease in the low category from 34.4% to 3.7%. There was no decrease in HRI rates both of intervention or control groups. Conclusion: Dengue Fever vector control training could decrease the value of BRI and Maya Index, but does not affect the value of HRI. The absence of changes in HRI  indicate that environmental hygiene and sanitation are factors that influence the probability of dengue fever occurrence. Keywords: Dengue Hemorrhagic Fever, Maya Index, training, vector control

IL-10-592 A/C Gene Polymorphism and Cytokine Levels are Associated with Susceptibility to Drug Resistance in Tuberculosis

2020 ◽  
Vol 8 (3) ◽  
pp. 103-112
Author(s):  
Atefeh SADEGHI SHERMEH ◽  
Majid KHOSHMIRSAFA ◽  
Ali-Akbar DELBANDI ◽  
Payam TABARSI ◽  
Esmaeil MORTAZ ◽  
...  
Keyword(s):  
Serum Levels ◽  
World Health ◽  
Control Group ◽  
Drug Resistant ◽  
Nucleotide Polymorphisms ◽  
Genotype Frequencies ◽  
Cytokine Levels ◽  
Ifn Γ ◽  
Health Organization ◽  
And Function

Introduction: Tuberculosis (TB) and especially resistant forms of it have a substantial economic burden on the community health system for diagnosis and treatment each year. Thus, investigation of this field is a priority for the world health organization (WHO). Cytokines play important roles in the relationship between the immune system and tuberculosis. Genetic variations especially single nucleotide polymorphisms (SNPs) impact cytokine levels and function against TB. Material and Methods: In this research SNPs in IFN-γ (+874 T/A) and IL-10 (-592 A/C) genes, and the effects of these SNPs on cytokine levels in a total of 87 tuberculosis patients and 100 healthy controls (HCs) were studied. TB patients divided into two groups: 1) 67 drug-sensitive (DS-TB) and 2) 20 drug-resistant (DR-TB) according to drug sensitivity test using polymerase chain reaction (PCR). For the genotyping of two SNPs, the PCR-based method was used and IFN-γ and IL-10 levels were measured by ELISA in pulmonary tuberculosis (PTB) and control group. Results: In -592A/C SNP, only two genotypes (AA, AC) were observed and both genotypes showed statistically significant differences between DR-TB and HCs (p=0.011). IL-10 serum levels in PTB patients were higher than HCs (p=0.02). The serum levels of IFN-γ were significantly higher in DS-TB patients than that of the other two groups (p<0.001); however, no significant differences were observed for allele and genotype frequencies in IFN-γ +874. Conclusions: Our results suggest that the SNP at -592 position of IL-10 gene may be associated with the susceptibility to DR-TB. However, further investigation is necessary. Keywords: Polymorphism, IFN-γ, IL-10, tuberculosis, drug-resistant tuberculosis

scholarly journals Pengaruh Pendidikan Kesehatan Metode Brainstorming dan Video terhadap Pengetahuan tentang Demam Berdarah pada Keluarga di Wilayah Kerja Puskesmas Puger Kabupaten Jember (The effect of Health Education Brainstorming Method and Video to the Knowledge about

2018 ◽  
Vol 6 (1) ◽  
pp. 141 ◽  
Author(s):  
Mukhammad Syafi`udin ◽  
Wantiyah Wantiyah ◽  
Kushariyadi Kushariyadi
Keyword(s):  
Treatment Group ◽  
Health Education ◽  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Group Treatment ◽  
Hemorrhagic Fever ◽  
Simple Random Sampling ◽  
T Test ◽  
Control Group ◽  
And Control

The increasing cases of dengue hemorrhagic fever disease in both children and adults can beinfluenced by several factors. One of that influences increasing of dengue fever is lack of communityknowledge about dengue hemorrhagic fever. Brainstorming is a form of discussion to gather theinformation from all participants. This research was to find out the effect of health educationbrainstorming method and video toward knowledge of dengue fever in the work area of Puger PublicHealth Center the distric of Jember. This was a quasy experiment research with two groups pretestposttesttreatment design. The population were 88 families with 30 respondents as sample,conducted by simple random sampling, and divided into two groups 15 respondents as treatmentgroup and 15 respondents as control group. Treatment group got health education brainstormingmethod combined with video and control group got health education brace method. Data wereanalyzed used t-test dependent and t-test independent with 95% CI (α: 0,05). The result showedthere were significant differences of knowledge before and after in both treatment group and controlgroup (p: 0,000) and there were significant differences between treatment group and control afterhealth education (p: 0,001). Overall, health education by brainstorming method and can improveknowledge of dengue hemorrhagic fever. It is recommended for nurses to do health educationpreventation and treatment about dengue hemorrhagic fever to improve knowledge people.Keywords: health education brainstorming method and video, knowledge, dengue hemorrhagicfever

scholarly journals Serum levels of interleukin-6, tumor necrosis factor-alpha and interferon-gama in infants with and without dengue

2008 ◽  
Vol 41 (1) ◽  
pp. 6-10 ◽  
Author(s):  
Berta Nelly Restrepo ◽  
Diana María Isaza ◽  
Clara Lina Salazar ◽  
Ruth Ramírez ◽  
Marta Ospina ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Primary Infection ◽  
Secondary Infection ◽  
Dengue Infection ◽  
Hemorrhagic Fever ◽  
Serum Levels ◽  
Tnf Alpha ◽  
Necrosis Factor Alpha ◽  
Ifn Gamma

This study compared the serum levels of IL-6, TNF-alpha and IFN-gamma, in children under 1 year of age with and without dengue. Sera were collected from a total of 41 children living in the Department of Antioquia, Colombia (27 patients with dengue and 14 controls). The results showed higher cytokine levels in children with dengue than without dengue, with statistically significant differences for IL-6 and IFN-gamma. No statistically significant differences were found between clinical forms, although IL-6 and IFN-gamma levels were higher in dengue fever cases than in dengue hemorrhagic fever cases. On the other hand, TNF-alpha levels were higher in dengue hemorrhagic fever than in dengue fever. The levels of IL-6 and TNF-alpha were higher in secondary infection than in primary infection, although IFN-gamma levels were higher in primary infection. These results suggest that IL-6, TNF-alpha and IFN-gamma are involved in dengue infection independently of the clinical form.

scholarly journals Proinflammatory Cytokines Correlate with Depression and Anxiety in Colorectal Cancer Patients

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Diego Oliveira Miranda ◽  
Taís Aparecida Soares de Lima ◽  
Lucas Ribeiro Azevedo ◽  
Omar Feres ◽  
José Joaquim Ribeiro da Rocha ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Proinflammatory Cytokines ◽  
Molecular Mechanisms ◽  
Depression Scale ◽  
Serum Levels ◽  
Anxiety And Depression ◽  
Control Group ◽  
Depression And Anxiety ◽  
Tnf Α ◽  
Cytokine Levels

The objective of this study was to investigate whether serum cytokine levels correlate with depression and anxiety in colorectal cancer (CRC) patients. Twenty patients hospitalized for surgical resection of CRC were included in the study group and twenty healthy volunteers comprised the control group. Depression and anxiety were analyzed using the Hospital Anxiety and Depression Scale (HADS), and serum levels of IL-1β, IL-6, IL-8, IL-10, IL-12, TNF-α, and TGF-βwere measured by Cytometric Bead Array. We found that more than half of CRC patients presented clinically significant levels of anxiety or depression, and 65% of them manifested a combination of severe anxiety and depression. CRC patients had increased serum levels of IL-1β, IL-6, IL-8, and TNF-α but lower IL-10 concentrations. Correlation analysis between HADS score and cytokine levels revealed a positive association of anxiety and/or depression with IL-1β, IL-6, IL-8, and TNF-α and a negative correlation with IL-10. These results indicate that circulating proinflammatory cytokines are involved in the pathophysiology of anxiety and depression in CRC patients. A better understanding of the molecular mechanisms involved in these psychological disorders will allow the design of therapeutic interventions that lead to an improved quality of life and overall survival of CRC patients.

scholarly journals Relationship between depression and blood cytokine levels in lung cancer patients

2018 ◽  
Vol 34 ◽  
pp. 113-115 ◽  
Author(s):  
Wen-Juan Liu ◽  
Xiao-Dan Wang ◽  
Wei Wu ◽  
Xiao Huang
Keyword(s):  
Lung Cancer ◽  
Cancer Patients ◽  
Rating Scale ◽  
Serum Levels ◽  
Advanced Lung Cancer ◽  
Control Group ◽  
Mild Depression ◽  
Lung Cancer Patients ◽  
Tnf Α ◽  
Cytokine Levels

Objective: To study the correlation between depression and blood cytokine levels in lung cancer patients.Methods: 92 patients with advanced lung cancer were evaluated for depression using the scoring index of depression self-rating scale. Lack of depression (n=24), mild depression (n=45), and moderate depression (n=23) were found in the cohort. Meanwhile, 40 healthy subjects were selected as the control group. The levels of IL-10, IL-6, IL-8, and TNF-α in each group were detected by sandwich enzyme-linked immunosorbent assays, and their correlation with the degree of depression was analyzed.Results: The levels of IL-10, IL-6, IL-8, and TNF-α were all higher than those in the control group (P<0.05). Moreover, the depression statuses of patients with lung cancer were positively correlated with IL-10, IL-6, and TNF-α levels (r = 0.705, 0.301, and 0.446, P<0.01); however, the level of IL-8 was not relevant (r=0.136, p>0.05).Conclusion: Serum levels of IL-10, IL-6, and TNF-α are associated with depression scoring in patients with lung cancer.

scholarly journals An initial investigation of serum cytokine levels in patients with gadolinium retention

2020 ◽  
Vol 53 (5) ◽  
pp. 306-313
Author(s):  
Holden T. Maecker ◽  
Weiqi Wang ◽  
Yael Rosenberg-Hasson ◽  
Richard C. Semelka ◽  
Joseph Hickey ◽  
...  
Keyword(s):  
Linear Models ◽  
Elevated Serum ◽  
Serum Levels ◽  
Control Group ◽  
Serum Cytokine ◽  
Serum Samples ◽  
Flu Vaccination ◽  
Gadolinium Retention ◽  
Cytokine Levels ◽  
The Difference

Abstract Objective: To determine whether individuals with proposed gadolinium deposition disease (GDD) have elevated serum levels of pro-inflammatory and pro-fibrotic cytokines, and whether specific cytokines are correlated with certain symptoms. Materials and Methods: Twenty-four participants recruited between May 2016 and June 2017 met GDD diagnostic criteria. The 64 control subjects provided serum samples before prophylactic flu vaccination. Serum cytokine levels were obtained with Luminex serum cytokine assay using eBiosciences/Affymetrix human 62-plex kits. Wilcoxon rank-sum tests were performed to assess the difference between the median fluorescence intensity values for the participants and the control group. Generalized linear models were built to evaluate the association between each cytokine of interest and selected participant symptoms. Results: Serum levels of 14 cytokines, including nine pro-inflammatory cytokines, were statistically significantly elevated compared to controls (p ≤ 0.05). Hypotheses regarding pro-fibrotic cytokines and cytokine links to specific symptoms’ intensity were not confirmed. Conclusion: The statistically significantly elevated cytokines may be markers of susceptibility to GDD or agents of symptom induction. These findings suggest that individuals developing symptoms characteristic of GDD after a contrast-assisted magnetic resonance imaging should be studied to investigate whether gadolinium retention and elevated cytokines may be related to their symptoms.

Effect of peanut oil and nandrolone on serum cytokines in pubertal term rats

2017 ◽  
Author(s):  
Erdal Tasgin ◽  
Seyfullah Haliloglu
Keyword(s):  
Serum Levels ◽  
Peanut Oil ◽  
Male Rats ◽  
Control Group ◽  
Nandrolone Decanoate ◽  
Serum Cytokine ◽  
Blood Samples ◽  
Serum Cytokines ◽  
Cytokine Levels ◽  
Cytokine Synthesis

The purpose of this study was to determine the effect of nandrolone on cytokine levels in pubertal term female and male rats. Totally 60 pubertal term Spraque Dawley rat (30/30 female/male) were divided into 3 equal groups. Control group received no treatment. PO group received intraperitoneally peanut oil every 5 days with an intervening gap of 2 days where no treatment was given. ND group received intraperitoneally nandrolone decanoate (10 mg/kg) within peanut oil like PO group. At the end of the four weeks, blood samples were obtained. Serum cytokine levels were measured by ELISA. Peanut oil significantly (P less than 0.05) increased serum levels of interleukin-1â and interleukin-6 in pubertal term male rats, while nandrolone decanoate had no any effect on the cytokine levels compared to experimental groups. It may be stated that nandrolone decanoate has no excessive inducer effect on the cytokine synthesis in pubertal male and female term.

scholarly journals Dengue viral infection deteriorates vitamin D3, K, thrombopoietin, and angiotensinogen levels in humans

2018 ◽  
Vol 16 ◽  
pp. 205873921879110 ◽  
Author(s):  
Hina Fatima ◽  
Muhammad Riaz ◽  
Zahed Mahmood ◽  
Fatima Yousaf ◽  
Muhammad Shahid
Keyword(s):  
Dengue Fever ◽  
Hematological Parameters ◽  
Test Group ◽  
Serum Levels ◽  
Viral Disease ◽  
Blood Parameters ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Mortality And Morbidity ◽  
Elisa Kit

Dengue fever is the most significant arthropod-transmitted viral disease in terms of mortality and morbidity, affecting at least 50 million people around the world. It is commonly recognized as “breakbone” fever as it is mostly associated with severe muscle and joint pain. The objective of the study was to evaluate and assess the levels of selected vitamins and hormones in serum of dengue patients from Faisalabad, Pakistan. Estimation of serum levels of vitamins D3 and K was carried out through spectrophotometer, while concentration of serum hormones, angiotensinogen and thrombopoietin, was determined through enzyme-linked immunosorbent assay (ELISA) kit method. Hematological blood parameters were also investigated. Hematological parameters significantly ( P < 0.05) differ in dengue fever patients and normal control subjects. Vitamin D3 level was found significantly ( P < 0.05) decreased in test group (dengue patients) compared to control group (healthy individual). Vitamin K level was also reduced in test group compared to control group, but this reduction was nonsignificant ( P > 0.05). Among the tested hormones, angiotensinogen level was found significantly ( P < 0.05) lower in test group, while thrombopoietin was nonsignificantly ( P > 0.05) higher compared to control group. In conclusion, the results of the current study demonstrated the significance of tested vitamins and hormones along with blood parameters in monitoring the dengue infected patients.

Pidotimod and Immunological Activation in Individuals Infected with HIV

2021 ◽  
Vol 18 ◽  
Author(s):  
Claudio Ucciferri ◽  
Katia Falasca ◽  
Marcella Reale ◽  
Manuela Tamburro ◽  
Antonio Auricchio ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Cystatin C ◽  
Immune Activation ◽  
Serum Levels ◽  
Antiretroviral Drugs ◽  
Hiv Positive ◽  
Control Group ◽  
Salivary Iga ◽  
Infected People ◽  
Cytokine Levels

Background: The improvements in HIV infection therapy and the large availability of antiretroviral drugs have led to an increased survival among HIV infected people, and a simultaneously to a raised morbidity and mortality due to notAIDS-related events in this group compared to the general population. An increased systemic inflammation and a persistent immune activation play a pivotal role in determining high rates of non-AIDS comorbidities. In the last years, many natural or synthetic immunomodulatory molecules acting by different mechanisms have been conceived. Pidotimod is a synthetic dipeptide molecule showing immunomodulatory properties. The aim of this pilot study was to evaluate the effects of Pidotimod supplementation on residual inflammation in HIV infected population. Methods: Forty HIV positive individuals under cART were enrolled: 30 were treated with Pidotimod supplementation (study group) and 10 served as control group (without Pidotimod supplementation). For all participants, Cystatin C, PCR, ESR, microalbuminuria, TNF-α, INF-γ, IL-4, IL-10, IL1β, IL-18 and IL-2 were measured at enrolment (T0), 4 weeks after of Pidotimod supplementation (T1), and 4 weeks after completing supplementation (T2). Results: In HIV positive participants treated with Pidotimod, the evaluation of cytokine levels showed that IL-10, IFN gamma, and IL-4 were significantly higher at enrolment compared to the control group. The increase under Pidotimod treatment persisted after supplementation suspension, while the pro-inflammatory cytokines levels were reduced. Salivary IgA also increased during 4 weeks of supplementation and persisted at 4 weeks after completing supplementation. On the other hand , the Cystatin C and microalbuminuria levels decreased over the time, at greater extent the Cystatin C serum levels. Conclusions: The study findings showed that the HIV population receiving Pidotimod achieved a rebalancing of proinflammatory and anti-inflammatory cytokines as well as a significant reduction in cystatin C levels. The treatment further allowed for an increase in salivary IgA levels at all the analyzed times, as secondary event to a remodulation of the immunological status obtained with pidotimod. This approach could represent a new way to design new intervention strategies aimed at improving the persistent immune activation status in the virologically suppressed HIV population.

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