scholarly journals Evaluation of anti-bacterial activity of Dashapushpam in the form of Ghritham

2021 ◽  
Vol 12 (1) ◽  
pp. 222-227
Author(s):  
Sreedevy K ◽  
Praseetha P K
Keyword(s):  
Plant Extracts ◽  
Monsoon Season ◽  
Bacterial Activity ◽  
Individual Plant ◽  
Bacterial Strains ◽  
Plant Resource ◽  
Diffusion Technique ◽  
Ancient People ◽  
Micro Organisms ◽  
Gel Diffusion

The state Kerala in India is famous for its plant resource both culturally and medicinally. There is a cluster of ten sacred medicinal plants commonly known as Dashapushpam. These herbs are of great importance in the cold rainy season. Each plant of this group possesses many medicinal values. There are many formulations using these herbs. Ancient people knew the value of using these herbs in a cluster, so they included them in their diet to improve immunity in the monsoon season. There are many Ayurvedic texts which mention the uses of these sacred herbs. Formulations that use all the members of Dashapushpam are rare. The Dasapushpagritham is one such formulation taken from the text vishavaidhya jyostnika. The present work intends to evaluate the anti-bacterial property of Dashapushpam when used in an Ayurvedic formulation known as Dasapushpa Ghritham. The bacterial strains used as the test micro-organisms for the study was Pseudomonas aeruginosa and Bacillus cereus. The method of the anti-bacterial evaluation was done through agar-gel diffusion technique. The samples including the plant extracts possessed a varying level of anti-bacterial activity against these two bacteria, and their values obtained were compared with standard antibiotic amoxicillin. Based on these results, it was concluded that the anti-bacterial property of the Ayurvedic drug Dasapushpa Ghritham has significant value when compared to the drug base and other individual plant extracts. Hence the present study proves the significant usage of the Dashapushpam plants in various therapeutics used as an anti-microbial agent.

scholarly journals Anti Bacterial Activity of Dashapushpa Ghritham

2019 ◽  
Vol 8 (3S2) ◽  
pp. 296-300
Keyword(s):  
Monsoon Season ◽  
Bacterial Activity ◽  
Diffusion Method ◽  
Individual Plant ◽  
Significant Activity ◽  
Bacterial Strains ◽  
The People ◽  
Level Of Activity ◽  
Test Organisms ◽  
Medicinal Properties

Dashapushpam is a group of ten sacred plants famous for its cultural and medicinal properties and it is used by the people of Kerala, specially during the monsoon season. This study is to demonstrate the anti bacterial activity of the ten herbs when used as a drug called dashapushpa ghritham. The anti bacterial activity was evaluated against the bacterial strains pseudomonas aerugenosa and bacillus cereus by agar-gel diffusion method. All the extract showed various level of activity for this test organisms and their activity is compared with the standard antibiotic. This study shows that the drug has significant activity than the base and individual plant extract. This result encourages the studies for the significant usage of the ten herbs in various medicines used as anti-infective agent.

Isolation, Detection and Estimation of Various Amylase Producing Bacteria in Various Soil Samples

2021 ◽  
Vol 08 ◽  
Author(s):  
Mohd Amin Mir ◽  
Mohammad Waqar Ashraf ◽  
Altaf Hussain ◽  
Bilal Ahmad Mir
Keyword(s):  
Plant Extracts ◽  
Soil Samples ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Cholesterol Levels ◽  
Microbiological Methods ◽  
Ethanolic Extracts ◽  
Microbial Strains ◽  
Litmus Test ◽  
Micro Organisms

Background: Soil is an ultimate source of all types of nutrients, which have both biological and non-biological importance. Studies are being carried out to isolate various types of micro-organisms from soil which have much more importance. So in the present study, amylase producing bacteria have been isolated from various soil samples. Aim: The isolation, identification, and estimation of various microbial strains for α-amylase enzyme production and then the inhibition of the growth of these microbial stains. Methods: The bacterial strains were isolated and then identified by various microbiological methods, including Gram’s staining method followed by several biochemical methods such as, litmus test, Gelatin test and Urea agar media and by viable cells. Results: Altogether, three microbial strains were identified from the soil samples in the concerned study. The concerned strains include- Shigella, Proteus and Bacillus, respectively. The concerned microbial strains were then analyzed for the amount of amylase enzyme and it had been found that Bacillus sp produces much more amount of amylase followed by Shigella sp, and lesser amylase enzyme producing activity was found in Proteus sp. The isolated bacteria were then analysed for inhibition of their growth by water and ethanolic extracts of Cuminum cyminuni. Among the extracts, it had been found that water extracts exhibited more inhibiting capacity than the ethanolic extracts. The study also revealed that among the bacterial strains, the Shigella sp got much more affected by the concerned plant extracts followed by Proteus sp and least inhibition was observed against the Bacillus sp. Conclusion: As per the above study, it is being concluded that - three amylase producing bacteria viz Shigella, proteus, bacillus sp were isolated from the soil samples. These isolated microbial strains could be used for the decomposition of cholesterol levels in human in addition to other microbial activity. These isolated bacterial could sometimes be averse therefore their growth could be stopped by various biological and chemical substances like Gentamicin and by various Plant extracts viz, Cuminum cyminuni Plant.

scholarly journals Assessment of anti bacterial screening of Pongamia pinnata stem against bacterial species: An In-vitro approach

2019 ◽  
Vol 9 (1-s) ◽  
pp. 181-184
Author(s):  
Adithya Gadeela Manish ◽  
Nerella Mounika ◽  
Bakshi Vasudha ◽  
Boggula Narender
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Medicinal Plants ◽  
Bacterial Species ◽  
Bacterial Activity ◽  
Pongamia Pinnata ◽  
Bacterial Strains ◽  
Agar Diffusion ◽  
Diffusion Technique

Numerous studies have shown that aromatic and medicinal plants are sources of diverse nutrient and non-nutrient molecules which protect the human body against various pathogens. Nature has been a source of medicinal agents for thousands of years and a large number of modern drugs have been isolated from natural sources. Herbal medicine is the oldest known healthcare system known to mankind. India has rich medicinal plants of nearly 7500 species. Many medicinal plants were with a long history of use in folk medicine against a variety of diseases. Recently, many researchers have taken a great interest on medicinal plants for their phytochemical constituents and biological activities including anti microbial activity. The anti bacterial activity of the ethanolic crude stem extract of Pongamia pinnata against four bacterial species (Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Escherichia coli) was investigated, using agar diffusion technique. At concentrations ranging from 10-40 mg/mL, the ethanolic crude extract showed activity against the four bacteria (Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and E. coli) from 2 mm-20 mm, after 24 hours incubation. The present study showed the effectiveness of the crude plant extract against the tested bacterial strains and indicates the potential use of the extract as anti bacterial agent for the control of infectious diseases. Keywords:  Pongamia pinnata, anti bacterial activity, agar diffusion technique, bacterial strains, chloramphenicol.

Phyto-fabrication of Iron Nanoparticles: Characterization and Antibacterial Capacity

2020 ◽  
Vol 16 ◽  
Author(s):  
Asma S. Algebaly ◽  
Afrah E. Mohammed ◽  
Mudawi M. Elobeid
Keyword(s):  
Electron Microscopy ◽  
Plant Extracts ◽  
Large Scale ◽  
Iron Nanoparticles ◽  
Ethanolic Extract ◽  
Green Technology ◽  
Resistant Bacteria ◽  
Scale Production ◽  
Bacterial Strains ◽  
Plant Sources

Introduction: Fabrication of iron nanoparticles (FeNPs) has recently gained a great concern for their varied applications in remediation technologies of the environment. Objective: The current study aimed to fabricate iron nanoparticles by green technology approach using different plant sources, Azadirachta indica leaf and Calligonum comosum root following two extraction methods. Methods: Currently, a mixture of FeCl2 and FeCl3 was used to react with the plant extracts which are considered as reducing and stabilizing agents for the generation of FeNPs in one step. Different techniques were used for FeNPs identification. Results: Immediately after mixing of the two reaction components, the color changed to dark brown as an indication of safe conversion of Fe ions to FeNPs, that later confirmed by zeta sizer, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). FeNPs fabricated by C. comosum showed smaller size when compared by those fabricated by A. indica. Using both plant sources, FeNPs fabricated by the aqueous extract had smaller size in relation to those fabricated by ethanolic extract. Furthermore, antibacterial ability against two bacterial strains was approved. Conclusion: The current results indicated that, at room temperature plant extracts fabricated Fe ion to Fe nanoparticles, suggesting its probable usage for large scale production as well as its suitability against bacteria. It could also be recommended for antibiotic resistant bacteria.

Fixation and Inactivation of Staphylococcal Leukocidin by Phosphatidylcholine and Ganglioside G M1 in Rabbit Polymorphonuclear Leukocytes

1980 ◽  
Vol 29 (2) ◽  
pp. 678-684
Author(s):  
Masatoshi Noda ◽  
Iwao Kato ◽  
Toshiya Hirayama ◽  
Futami Matsuda
Keyword(s):  
Cholera Toxin ◽  
Carboxymethyl Cellulose ◽  
Polymorphonuclear Leukocytes ◽  
Receptor Site ◽  
Maximal Effect ◽  
Diffusion Technique ◽  
Subunit B ◽  
Gel Diffusion

Staphylococcal leukocidin is resolved by chromatography on carboxymethyl cellulose columns into two components, which are designated F (fast) and S (slow). Fixation and inactivation of both components were studied as follows. (i) Leukocidin activity was confined to the first 10 min of intoxication, and the maximal effect resulted from treating 10 6 rabbit peripheral polymorphonuclear leukocytes per 20 μl with 0.5 ng of each component of leukocidin. The S component was more responsible for the interaction with the leukocytes than the F component. (ii) The F component was inactivated by phosphatidylcholine at concentrations which corresponded to molar proportions of 1:1 and bound to [ 14 C]phosphatidylcholine at equimolar proportions. (iii) The S component was inactivated by ganglioside G M1 at 1:1 molar proportions, but not by any of the related glycolipids. Ganglioside G M1 also was precipitated with the S component by a gel diffusion technique. Subunit B of cholera toxin competitively inhibited the binding of the S component to rabbit leukocyte membranes. This indicates that ganglioside G M1 may resemble or be part of the receptor site for the S component.

scholarly journals Case series and review of Ayurvedic medication induced liver injury

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Christopher M. Karousatos ◽  
Justin K. Lee ◽  
David R. Braxton ◽  
Tse-Ling Fong
Keyword(s):  
Liver Injury ◽  
Plant Extracts ◽  
Significant Proportion ◽  
Case Series ◽  
The United States ◽  
Tinospora Cordifolia ◽  
Individual Plant ◽  
Medicine Use ◽  
Drug Induced ◽  
Drug Induced Liver Injury

Abstract Background Complementary and alternative medicine use among Americans is prevalent. Originating in India, Ayurvedic medicine use in the United States has grown 57% since 2002. CAM accounts for a significant proportion of drug induced liver injury in India and China, but there have been only three reports of drug induced liver injury from Ayurvedic medications in the U.S. We report three cases of suspected Ayurvedic medication associated liver injury seen at a Southern California community hospital and review literature of Ayurvedic medication induced liver injury. Case presentations Three patients presented with acute hepatocellular injury and jaundice after taking Ayurvedic supplements for 90–120 days. First patient took Giloy Kwath consisting solely of Tinospora cordifolia. Second patient took Manjishthadi Kwatham and Aragwadhi Kwatham, which contained 52 and 10 individual plant extracts, respectively. Third patient took Kanchnar Guggulu, containing 10 individual plant extracts. Aminotransferase activities decreased 50% in < 30 days and all 3 patients made a full recovery. Roussel Uclaf Causality Assessment Method (RUCAM) scores were 7–8, indicating probable causality. These products all contained ingredients in other Ayurvedic and traditional Chinese medicines with previously reported associations with drug induced liver injury. Conclusions These patients highlight the risk of drug induced liver injury from Ayurvedic medications and the complexity of determining causality. There is a need for a platform like LiverTox.gov to catalog Ayurvedic ingredients causing liver damage.

scholarly journals ANTIBACTERIAL ACTIVITY OF METHANOLIC RHIZOME EXTRACT OF ALPINIA CALCARATA ROSC. (ZINGIBERACEAE)

2016 ◽  
Vol 3 (1) ◽  
pp. 65-66
Author(s):  
Arumugasamy K ◽  
Nantha Kumar R ◽  
Abdul Kaffoor H ◽  
Shalimol A
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antibacterial Activities ◽  
Bacterial Activity ◽  
Proteus Vulgaris ◽  
Bacterial Strains ◽  
Zone Of Inhibition

The methanolic rhizome extract of A. calcarata was evaluated for its antibacterial activities against five bacterial strains Pseudomonas aeuroginosa, Proteus vulgaris, Salmonella paratyphi, Bacillus thurungiensis and Staphylococcus faccealis. The extract has inhibited all the tested bacterial species with different manner at various concentration. However the higher level zone of inhibition in 400 (mg/ml) is significant against all the above said bacterial strains of these Salmonella paratyphi. Based on the present study it can be conculuded that the plant rhizome possess potent anti bacterial activity.

scholarly journals Assessment of analgesic and antipyretic activity of traditional formula used in the treatment of seasonal infections

2013 ◽  
Vol 2 (9) ◽  
pp. 143-147
Author(s):  
Kugalur Ganesan Parthiban ◽  
Senthil Kumar Natesan ◽  
Ganesan Sekar ◽  
Krishnamoorthi Mahalakshm
Keyword(s):  
Plant Extracts ◽  
Rural Areas ◽  
Dosage Form ◽  
Pharmaceutical Journal ◽  
Albino Rats ◽  
Individual Plant ◽  
Significant Activity ◽  
Plant Materials ◽  
Antipyretic Activity ◽  
Liquid Dosage Form

Traditional treatments and medicines are the main sometimes the only source of health care for millions of people living in rural areas of developing countries. The aim of the present study is to investigate the analgesic and antipyretic activity of individual plant extracts and poly-herbal formula [PHF] made in to liquid dosage form of three different doses in albino rats. The plant materials were extracted in boiling distilled water for six hours, filtered, concentrated and dried. The aqueous extracts were prepared in to poly-herbal liquid dosage form by using water, glycerol mixture and acacia 2% in three different preparation of varying extract doses.1ml of above preparations orally fed for investigation. Pentozocine and paracetamol was used as standard drugs respectively. For individual plants the extracts were suspended in 2% acacia solution and performed. The results of analgesic and anti-pyretic activity of PHF 1 and PHF 2 showed significant results (P<0.01 and P<0.05 respectively) and PHF3 did not show any significant results. In the individual plant extracts slightly significant activity (P<0.05) was observed.DOI: http://dx.doi.org/10.3329/icpj.v2i9.16075 International Current Pharmaceutical Journal, August 2013, 2(9): 143-147

scholarly journals A bioassay technique to assess the molluscicidal effects of microbes

2009 ◽  
Vol 62 ◽  
pp. 7-11 ◽  
Author(s):  
S.A. Howlett ◽  
G. Burch ◽  
U. Sarathchandra ◽  
N.L. Bell
Keyword(s):  
Insect Pests ◽  
Biocontrol Agents ◽  
Bacterial Strains ◽  
Pastoral Systems ◽  
Significant Damage ◽  
Oat Bran ◽  
Bioassay Technique ◽  
Pathogenic Microbes ◽  
Micro Organisms ◽  
Retail Products

Terrestrial slugs (Order Stylommatophora) can cause significant damage in cropping and pastoral systems Pathogenic microbes have long been shown to have potential as biocontrol agents for insect pests and a number of retail products are available but there is no equivalent product for molluscs A research program is underway to investigate the potential of micro organisms as slug biocontrol agents and this paper describes a bioassay methodology that can be used to assess the molluscicidal properties of bacterial isolates Its feasibility has been demonstrated in laboratory tests against pest slugs of the genus Deroceras Fourteen bacterial strains were screened by feeding them to the slugs in a mix based on oat bran a food that is highly palatable to these molluscs Mortality was assessed 3 and 4 days after introduction of the treated food Two of the bacterial strains tested resulted in significant mortality killing 100 slugs within 4 days

scholarly journals Phytochemical Screening and Biological Studies of Shilajit (Asphaltum)

2017 ◽  
Vol 9 (1) ◽  
pp. 15
Author(s):  
Shahid Aziz ◽  
Sidra Khaliq ◽  
Habib Ur-Rehman ◽  
Kh. Shakeel Ghani ◽  
Muhammad Irshad ◽  
...  
Keyword(s):  
Microbial Activity ◽  
Cardiac Glycosides ◽  
Reducing Sugars ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Biological Studies ◽  
Dead Plant ◽  
Micro Organisms

<p>Shilajit (asphaltum)  is produced  by the long term humification  of dead plant material  and organic vegetable matter  by different micro-organisms and has great potential for the treatment of a variety of human conditions.  This treatise reviews its origin, sources, chemical composition, biological  and  commercial importance. Phytochemical analysis was done by standard methods to evaluate  different Shilajit (asphaltum) classes of compounds in different samples of shilajit  which are responsible for their  biological activity.  Shilajit`s anti-microbial activity has been evaluated  against four different bacterial strains viz., <em>Escherichia coli, Psuedomonas aeuroginosa</em>, <em>Klebisella pneumonia</em> and <em>Staphylococcus aureus. </em>Phytochemical analysis illustrated  that shilajit contains  terpenoids, cardiac glycosides, saponins and reducing sugars. Surprisingly,  some classes of compounds are absent in shilajit  viz., alkaloids, flavonoids, tannins and anthraquinones. . Shilajit showed no response towards halophytic bacteria and  negligible activity was shown towards other strains of bacteria. Since   anti-microbial activity is based on environmental factors  its activity varied  between locations.</p>

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