scholarly journals HEMATOLOGI PERBANDINGAN HEWAN VERTEBRATA: LELE (Clarias batracus), KATAK (Rana sp.), KADAL (Eutropis multifasciata), MERPATI (Columba livia) DAN MENCIT (Mus musculus)

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Diah Wulandari Rousdy ◽  
Riza Linda
Keyword(s):  
Mus Musculus ◽  
Animal Species ◽  
Leukocyte Count ◽  
Columba Livia ◽  
Erythrocyte Count ◽  
Hematological Profile ◽  
Hematology Parameters ◽  
Animal Taxon

ABSTRACTHematology studies between Vertebrate classes can provide supporting data related to animal activities and adaptation to their habitat. Low Vertebrate ektoterm showed different hemotological profil than endoderm Vertebrate. The aims of this study is to compare hematological profile between Vertebrate which includes five classes animal taxon. Animal species was taken randomly, considered to represent the five classes taxon: Clarias batracus from Class Pisces, Rana sp. from Class Amphibia, Eutropis multifasciata from Class Reptilia, Columba livia from Class Aves and Mus musculus from Class Mammalia. Hematology parameters were observed was hemoglobins, HCT, erythrocyte count, leukocyte count, leukocyte differential, MCV, MCH and MCHC. The result showed Vertebrate from Class Pisces (catfish), amphibian (frogs) and reptiles (lizards) had hemoglobin, hematocrit, erythrocytes higher than Aves and Mammals. Leucocytes count in Class Pisces, Amphibians, Reptiles and Aves range 12.000-19.000 cells/mL higher than mammal leukocyte 5000 cells/mL. Differential leukocyte in catfish, frogs, lizards, pigeons and mice have the highest percentage of lymphocytes (37-62%). Keywords: catfish, frog, hematology, lizard, mice, pigeon, vertebrate ABSTRAKStudi hematologi hewan kelas Vertebrata dapat memberikan data pendukung terkait aktivitas hewan dan adaptasi terhadap habitatnya. Hewan Vertebrata tingkat rendah yang ektoterm mempunyai profil hematologi yang berbeda dengan Vertebrata tinggi yang sebagian besar endoterm. Tujuan dari penelitian ini adalah untuk membandingkan profil hematologi antar hewan Vertebrata yang meliputi lima kelas hewan takson. Hewan sampel diambil secara acak dari populasi hewan yang mewakili lima kelas takson: Clarias batracus dari kelas Pisces, Rana sp. dari kelas Amfibi, Eutropis multifasciata dari kelas Reptil, Columba livia dari kelas Aves dan Mus musculus dari kelas Mammalia. Parameter hematologi yang diamati adalah hemoglobin, hematokrit, jumlah eritrosit, jumlah leukosit, leukosit diferensial, MCV, MCH dan MCHC. Hasil pengukuran hematologi menunjukkan Vertebrata dari kelas Pisces (lele), Amfibi (katak) dan Reptil (kadal) memiliki hemoglobin, hematokrit, eritrosit lebih tinggi dari pada Aves dan Mamalia. Jumlah leukosit di kelas Pisces, Amfibi, Reptil, dan Aves berkisar 12.000-19.000 sel/mL lebih tinggi dari leukosit mamalia yakni 5000 sel/mL. Pengamatan leukosit diferensial pada ikan lele, katak, kadal, merpati, dan tikus menunjukkan persentase limfosit tertinggi (37-62%) dibanding jenis leukosit lain. Kata kunci: ikan lele, katak, hematologi, kadal, mencit, merpati, vertebrata

Blood in psoriasis. Engman, Davis, Reinsh (Diss., 1921)

1923 ◽  
Vol 19 (4) ◽  
pp. 103-103
Author(s):  
E. Korneychik
Keyword(s):  
Leukocyte Count ◽  
Erythrocyte Count ◽  
Total Leukocyte Count ◽  
Normal Limits ◽  
Transient Decrease

The author concludes that Hb content in psoriasis is sometimes decreased, especially in the rash period, erythrocyte count is usually normal, but sometimes there is a transient decrease in the rash period, total leukocyte count is within normal limits.

scholarly journals Hematological profile of captive bearded capuchin monkeys (Sapajus libidinosus) from Northeastern Brazil

2018 ◽  
Vol 48 (10) ◽  
Author(s):  
Adriano Fernandes Ferreira ◽  
Felisbina Luísa Queiroga ◽  
Rinaldo Aparecido Mota ◽  
Eneida Willcox Rêgo ◽  
Stéphanie Machado Mota ◽  
...  
Keyword(s):  
Reference Values ◽  
Hematological Parameters ◽  
Age Factors ◽  
Erythrocyte Count ◽  
Northeastern Brazil ◽  
Capuchin Monkeys ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Hematological Profile ◽  
In Captivity

ABSTRACT: Bearded Capuchin or Black-striped Capuchin monkeys (Sapajus lidibinosus) are New World robust capuchin monkeys widely used in medical research. Few data are available concerning hematological reference values for these species, with no studies available from the Northeast region in Brazil. The aim of this study was to determine the hematological reference values for healthy bearded capuchin monkeys and to analyze the influence of sex and age factors. Blood samples were collected from 50 healthy bearded capuchin monkeys housed in captivity. These were analysed for total erythrocyte, hemoglobin, leukocyte and platelet count, packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC). When considering the age factor, significant differences were reported for total erythrocyte count, PCV, hemoglobin, total leucocytes, band neutrophils, eosinophils and lymphocytes (higher in juveniles). Significant sex-associated differences were noted for total erythrocyte count, PCV, hemoglobin (higher in males) and number of lymphocytes (higher in females).We have reported for the first time the hematological profile of bearded capuchin monkeys in captivity in the state of Paraíba, Brazil. These results can contribute for a better understanding of the normal physiology of capuchin monkeys, while demonstrating that factors such as sex and age influence hematological parameters should be taken into consideration in the hematological evaluation of this species.

scholarly journals Effect of garlic and vitamin B-complex in lead acetate induced toxicities in mice

1970 ◽  
Vol 6 (2) ◽  
pp. 203-210 ◽  
Author(s):  
MSH Khan ◽  
M Mostofa ◽  
MS Jahan ◽  
MA Sayed ◽  
MA Hossain
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Lead Acetate ◽  
Leukocyte Count ◽  
Erythrocyte Count ◽  
Total Leukocyte Count ◽  
Vitamin B ◽  
Group B ◽  
Haemoglobin Content ◽  
Serum Glutamate

The experiment was performed on 25 adult mice, (Swiss albino), weighing about 25 gm, maintained on standard pellet diet and drinking water ad libitum. All the mice were randomly divided into five equal groups (5x5). Each group comprised of five mice was marked as group A, B, C, D and E. In the present study an attempt has been taken to study the effect of Garlic and vitamin B-complex in lead induced toxicities in mice with the observation of some parameters as toxic signs and body weight, some haematological parameters like total erythrocyte count (TEC), total leukocyte count (TLC), haemoglobin content (Hb%) and Packed cell volume (PCV), some biochemical parameters such as serum glutamate pyruvate transaminase SGPT/ALT, Serum glutamate Oxaloacetate transaminase (SGOT/AST) and postmortem changes in mice. An attempt was also made to estimate the residual deposition of lead in body tissues i.e. blood, liver, kidney, brain and bone in mice. Mice treated with lead acetate showed severe toxic signs and significantly reduced total erythrocyte count, total leukocyte count, haemoglobin content and packed cell volume and significant elevation of SGPT and SGOT. But in case of mice treated by lead acetate along with Garlic and vitamin B-complex showed almost normal levels of haematological and biochemical parameters. From this experiment it is revealed that Garlic and vitamin B-complex has protective and curative effect in lead toxicity. The mean body weight of mice of group B (only lead acetate) was significantly (p < 0.01) decreased, in comparison with the other treated groups. Lead acetate group also significantly reduced (p < 0.01) total erythrocyte count, packed cell volume and hemoglobin content was on 42 days of treatment. SGPT and SGOT were significantly elevated in all treated groups in comparison with control group. The liver of the mice of group B were blackish discoloration and enlarged. The significant reduction of lead in blood, liver and kidney was found in group E < C< D in comparison to group B (lead treated). The order of effectiveness was Garlic + Vitamin B- complex > Garlic > Vitamin Bcomplex. Key words: Garlic, vitamin B-complex, lead acetate, mice   doi: 10.3329/bjvm.v6i2.2337 Bangl. J. Vet. Med. (2008). 6 (2): 203-210

Comparative hematology of wild Anguilliformes (Muraena helena, L. 1758, Conger conger, L. 1758 and Anguilla anguilla L. 1758)

2013 ◽  
Vol 63 (1) ◽  
pp. 77-92 ◽  
Author(s):  
Domagoj Ðikić ◽  
Duje Lisičić ◽  
Sanja Matić-Skoko ◽  
Pero Tutman ◽  
Daria Skaramuca ◽  
...  
Keyword(s):  
Cell Count ◽  
Adriatic Sea ◽  
Leukocyte Count ◽  
Anguilla Anguilla ◽  
Cell Types ◽  
Erythrocyte Count ◽  
Cell Counts ◽  
Staining Methods ◽  
Senescent Erythrocytes ◽  
Conger Conger

The objective of this study was to compare circulating blood cell counts and morphology of three eel species: Muraena helena (moray), Conger conger (European conger) and Anguilla anguilla (European common eel). Moray and conger were collected from the Adriatic Sea at the Elaphite Islands near Dubrovnik, Croatia; common eels were collected in the Neretva River, Croatia. Hematological comparison was conducted using Natt-Harrick’s and May-Grünwald Giemsa staining methods. The hematocrit of moray and conger were similar, while common eel had higher values by 60%. Manual cell count showed that common eel had the highest erythrocyte count. Conger had a higher erythrocyte count than moray, with a higher percentage of proerythrocytes and senescent erythrocytes compared to moray and common eel. The leukocyte count was similar in common eel and moray and slightly lower in conger. The thrombocyte count was highest in conger and lowest in moray. In all three species, the neutrophil (heterophil) nuclei appeared as either circular or bi-lobed. Moray had the highest neutrophil (heterophil) percentage and a subtype with intensively basophilic cytoplasm appearing in a similar percentage as the normal type. In common eel, neutrophils (heterophils) were the only detected granulocytes. Basophils were detected in conger eels. Eosinophils were not detected in any of the sampled fish. The size of all cell types in moray was slightly larger than in other two species. In conclusion, our findings reveal major differences in the cell count and diversity in cell subtypes between three kin species of eels.

scholarly journals PROFIL FITOKIMIA DAN PENGARUH EKSTRAK TANGKAI DAUN TALAS KEMUMU (Colocasia gigantea Hook.f ) TERHADAP JUMLAH LEUKOSIT Mus musculus

Alotrop ◽  
2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Rofiqa Asri ◽  
Dewi Handayani ◽  
Agus Sundaryono
Keyword(s):  
Secondary Metabolite ◽  
Mus Musculus ◽  
Leukocyte Count ◽  
Anova Analysis ◽  
Leukocyte Counts ◽  
Phytochemical Profile ◽  
Blood Leukocyte ◽  
Method Of Extraction ◽  
Difference Test ◽  
One Way Anova

This research aims to identify secondary metabolite compounds in Talas kemumu leaf stalk (Colocasia gigantea Hook.f) and to determine the effect of extract on the number of male Mus musculus leukocytes that have been induced aspirin. Aspirin is used to increase the number of M.musculus leucocytes.The method used in this research is the method of extraction by maceration to attract secondary metabolites of active compounds  C.gigantea leaf stalk and the measurement of blood leukocyte counts are calculated using a hemocytometer (improved neubauer). Test of the effect of the leaf stalk of C.giganteaextract on the number leukocytes of M.musculus  was performed by dividing into 5 groups with 5 replications according to the Federer formula, namely K (-) were given distilled water, K (+) were given aspirin, P1, P2 and P3 are each given a dose of extract of  C.gigantea leaf stalk of 0,028 g/KgBB, 0,056 g/KgBB and 0,084 g/KgBB. The results showed that the identification of phytochemical profile in C.gigantea leaf stalk contains secondary metabolite compounds such as alkaloids, flavonoids, terpenoids, tannins and saponins. The results shows that giving of C.gigantea leaf stalk extract can reduce the number of leukocytes M.musculus which have induced aspirin. The giving of C.gigantea leaf stalk extract can decrease the number of aspirin-induced M.musculus leukocytes. This is evident from the results of One Way Anova analysis and the Smallest Real Difference Test (BNT) because in each treatment group has a significant influence. The effective dose that has the greatest activity in decreasing the number of M.musculus leukocytes found in the P1 treatment of 0,028 g /KgBB can decrease the blood leukocyte count by 6,830 / mm3.

scholarly journals Hematological Value of Captive Elephants (Elephas maximus) Around Chitwan National Park, Sauraha, Nepal

2021 ◽  
Author(s):  
Roshan Ghimire ◽  
Sagar Regmi ◽  
Rakshya Shrestha ◽  
Amir Sadaula ◽  
Janardan Dev Joshi
Keyword(s):  
National Park ◽  
Leukocyte Count ◽  
Hematological Parameters ◽  
Research Work ◽  
Health Condition ◽  
Erythrocyte Count ◽  
Total Leukocyte Count ◽  
Major Work ◽  
Significant Difference ◽  
Chitwan National Park

Abstract Veterinary hematology serves as an important screening procedure to assess general health condition, diagnosis, and treatment of disease. The study was aimed to interpret and establish a set of hematology reference range for Asian elephants kept under private and government facilities of Nepal as no major work has been done till now. Blood samples from 50 elephants around Chitwan National Park, Sauraha was collected and hematological parameters like total erythrocyte count and total leukocyte count were determined. The results showed that the majority of hematological value was in line with the value previously published by different authors. The mean erythrocyte and leukocyte count was reported as 3.32 ±0.93 ×10^6 cell/µL and 10448±335.49 cells/ µL respectively. No sex-associated significant difference was observed in the case of total erythrocyte count whereas total leukocyte counts varied significantly within sex. Our finding revealed no significant difference in hematological parameters between governmental and privately owned elephants. The hematological value of our study can be used as a reference value for assessing the health condition of elephants in Nepal. Further research work should be conducted to evaluate the factors affecting hematological parameters.

scholarly journals Pigeons (Columba livia domestica) Are Susceptible to Infectious Bursal Disease: A Comparative Study of Their Hematological and Serum Biochemical Alterations

2021 ◽  
Vol 8 ◽  
Author(s):  
Ochuko Orakpoghenor ◽  
Sunday Blessing Oladele ◽  
Paul Ayuba Abdu ◽  
Talatu Patience Markus ◽  
Samson James Enam ◽  
...  
Keyword(s):  
Leukocyte Count ◽  
Columba Livia ◽  
Infectious Bursal Disease ◽  
Total Leukocyte Count ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Serum Total Protein ◽  
Biochemical Alterations ◽  
Bursal Disease ◽  
Serum Biochemical

The hematological and serum biochemical alterations following very virulent infectious bursal disease virus (IBDV) infection in pigeons and chickens were evaluated in this study. Sixty IBDV seronegative birds comprising 30 (3–6 weeks old) pigeons and 30 (3 weeks old) chickens were randomly divided as follows: 10 uninoculated pigeons only, 10 inoculated pigeons + 10 sentinel chickens, 10 inoculated chickens + 10 sentinel pigeons, and 10 uninoculated chickens. Inoculated birds were administered 0.20 ml of inoculum containing very virulent IBDV (vvIBDV). Blood was collected postinoculation/exposure (pi/pe) and processed for hematology and biochemistry. The results revealed significantly (P &lt; 0.05) increased packed cell volume, decreased mean corpuscular hemoglobin (MCH), and MCH concentration (MCHC) in inoculated and sentinel chickens. Total leukocyte count (TLC), heterophil, and heterophil/lymphocyte (H/L) ratio were increased from 3 dpi/dpe in inoculated pigeons and from 3 to 14 dpi/dpe in inoculated and sentinel chickens. At 10 and 14 dpi/dpe, there was significantly (P &lt; 0.05) increased serum total protein and globulin concentrations and decreased albumin/globulin ratio in pigeons and chickens. Serum urea concentration showed significant (P &lt; 0.05) increase in inoculated and sentinel chickens at 3 and 4 dpi/dpe. To the authors' knowledge, this is the first report on hematological and serum biochemical alterations due to vvIBDV infection in pigeons.

scholarly journals Medicinal Animals and Plants as Alternative and Complementary Medicine in Southern Regions of Khyber Pakhtunkhwa, Pakistan

2021 ◽  
Vol 12 ◽  
Author(s):  
Sakina Mussarat ◽  
Rehman Ali ◽  
Shandana Ali ◽  
Ramzi A. Mothana ◽  
Riaz Ullah ◽  
...  
Keyword(s):  
Complementary Medicine ◽  
Animal Species ◽  
Columba Livia ◽  
Local Communities ◽  
Piper Nigrum ◽  
Animal Products ◽  
Khyber Pakhtunkhwa ◽  
Alternative And Complementary Medicine ◽  
Plant Mixture ◽  
Pharmacologically Active

Background: Local communities use animals and plants as common traditional therapies for various diseases. The study aimed to document animals and animal-plant mixture recipes that are used as alternative and complementary medicine in southern regions of Khyber Pakhtunkhwa, Pakistan.Methods: The data were collected (2017–2018) in three remote areas (Dera Ismail Khan, Bannu, and Lakki Marwat) through questionnaires and face-to-face interviews with local inhabitants. Data on ethnomedicinal uses and cultural values of animal products or parts and their mixture with plants were analyzed using various indices such as frequency of citation (FC), informant consensus (FIC), and fidelity level (FL) to find the highly preferred species in the area.Results: A total of 185 informants (117 females and 68 males) were interviewed. The study documented 32 animal species, vertebrates (n = 24) and invertebrates (n = 8), for curing 37 types of diseases. Mammals (n = 13) were among the most commonly utilized species followed by birds (n = 8), arthropods (n = 7), reptiles (n = 2), and fishes and annelids (n = 1 each). Among the reported animals, Herpestes edwardsi (mongoose), Macaca mulatta (monkey), Labeo rohita (rohu), Oryctolagus cuniculus (rabbit), and Streptopelia decaocto (dove) were the newly reported species used as alternative medicine. The meat of Capra hircus (goat), monkey, and rabbit was used to treat chronic diseases such as hepatitis C, cancer, epilepsy, and asthma. A total of 17 plants belonging to 15 botanical families were used in combination with animal parts/products. The commonly used families were Piperaceae (31%) followed by Apiaceae (27%). The notable plant species in combination with animal products were Curcuma longa, Piper nigrum, Coriandrum sativum, Brassica rapa, and Phoenix dactylifera. Seeds were the highest used part in animal-plant mixture recipes. Gallus gallus (chicken) and Columba livia (pigeon) secured the highest (FC = 28) and (FL = 80%), respectively. FIC results had shown the highest degree of consensus for general body weakness (FIC = 0.88) and pyrexia (FIC = 0.86).Conclusion: Our findings suggest that local communities in the southern regions of Khyber Pakhtunkhwa have substantial knowledge about the formulation of ethnomedicines from both flora and fauna that need urgent documentation to avoid eroding and for conservational purposes. The newly reported phytozootherapeutic recipes and animal species can potentially be a source of pharmacologically active constituents and should be checked experimentally for further confirmation.

scholarly journals Effectiveness of Potassium Diformate on Feed to Improve Immune Performance of Goldfish (Carassius auratus L.)

2020 ◽  
pp. 24-33
Author(s):  
Ayi Yustiati ◽  
Rima Tri Wahyuni ◽  
Achmad Rizal ◽  
Ibnu Bangkit Bioshina Suryadi
Keyword(s):  
Survival Rate ◽  
Red Blood Cells ◽  
Clinical Sign ◽  
Blood Cells ◽  
Carassius Auratus ◽  
Leukocyte Count ◽  
White Blood Cells ◽  
Erythrocyte Count ◽  
Total Leukocyte Count ◽  
Goldfish Carassius Auratus

This research aims to determine the optimum potassium diformate (KDF) dosage which was added to feed to increase the immune status of goldfish (Carassius auratus L). This study was conducted from September 31 - November 15, 2019 at the Laboratory of Aquaculture and Molecular Biotechnology Laboratory of the Faculty of Fisheries and Marine Sciences, University of Padjadjaran. The research method used is the experimental Complete Random Design (CRD) with 4 treatments and 3 replications. The treatments are A (without KDF/control), B (0.1% KDF), C (0.3% KDF) and D (0.5% KDF). The observed parameters are survival rate, total leukocyte count (white blood cells), total erythrocyte count (red blood cells) and gross clinical sign. Observations were made after 30 days of KDF and post-test challenge by Aeromonas hydrophila for 14 days. Data on the number of total leukocyte count and total erythrocyte count were analyzed using F test and Duncan's advanced test at a 95% confidence level, while the gross clinical sign data were analyzed descriptively. The results showed that the KDF 0.3% was the optimum dose and was very effective in optimizing the performance of the goldfish immune system optimally, seen from the number of white blood cells and red blood cells which experienced the highest increase of 23.48% and 9.30%. After the challenge test, fish that were given KDF 0.3% had the highest survival rate of 46.67%. In addition, the process of recovering the number of white blood cells and red blood cells can be faster than other treatments, with a value of 12.85×104 cells mm3-1 and 0.96×106 cells mm3-1, marked also by the healing of clinical symptoms in the morphology of goldfish.

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