scholarly journals PROFIL FITOKIMIA DAN PENGARUH EKSTRAK TANGKAI DAUN TALAS KEMUMU (Colocasia gigantea Hook.f ) TERHADAP JUMLAH LEUKOSIT Mus musculus

Alotrop ◽  
2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Rofiqa Asri ◽  
Dewi Handayani ◽  
Agus Sundaryono
Keyword(s):  
Secondary Metabolite ◽  
Mus Musculus ◽  
Leukocyte Count ◽  
Anova Analysis ◽  
Leukocyte Counts ◽  
Phytochemical Profile ◽  
Blood Leukocyte ◽  
Method Of Extraction ◽  
Difference Test ◽  
One Way Anova

This research aims to identify secondary metabolite compounds in Talas kemumu leaf stalk (Colocasia gigantea Hook.f) and to determine the effect of extract on the number of male Mus musculus leukocytes that have been induced aspirin. Aspirin is used to increase the number of M.musculus leucocytes.The method used in this research is the method of extraction by maceration to attract secondary metabolites of active compounds  C.gigantea leaf stalk and the measurement of blood leukocyte counts are calculated using a hemocytometer (improved neubauer). Test of the effect of the leaf stalk of C.giganteaextract on the number leukocytes of M.musculus  was performed by dividing into 5 groups with 5 replications according to the Federer formula, namely K (-) were given distilled water, K (+) were given aspirin, P1, P2 and P3 are each given a dose of extract of  C.gigantea leaf stalk of 0,028 g/KgBB, 0,056 g/KgBB and 0,084 g/KgBB. The results showed that the identification of phytochemical profile in C.gigantea leaf stalk contains secondary metabolite compounds such as alkaloids, flavonoids, terpenoids, tannins and saponins. The results shows that giving of C.gigantea leaf stalk extract can reduce the number of leukocytes M.musculus which have induced aspirin. The giving of C.gigantea leaf stalk extract can decrease the number of aspirin-induced M.musculus leukocytes. This is evident from the results of One Way Anova analysis and the Smallest Real Difference Test (BNT) because in each treatment group has a significant influence. The effective dose that has the greatest activity in decreasing the number of M.musculus leukocytes found in the P1 treatment of 0,028 g /KgBB can decrease the blood leukocyte count by 6,830 / mm3.

scholarly journals Prediction of leukocyte counts during paediatric acute lymphoblastic leukaemia maintenance therapy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Santeri Karppinen ◽  
Olli Lohi ◽  
Matti Vihola
Keyword(s):  
Maintenance Therapy ◽  
Acute Lymphoblastic Leukaemia ◽  
Lymphoblastic Leukaemia ◽  
Leukocyte Count ◽  
Peripheral Blood Leukocyte ◽  
Reactive Protein ◽  
Leukocyte Counts ◽  
Nonlinear State Space Models ◽  
Blood Leukocyte ◽  
The Individual

AbstractMaintenance chemotherapy with oral 6-mercaptopurine and methotrexate remains a cornerstone of modern therapy for acute lymphoblastic leukaemia. The dosage and intensity of therapy are based on surrogate markers such as peripheral blood leukocyte and neutrophil counts. Dosage based leukocyte count predictions could provide support for dosage decisions clinicians face trying to find and maintain an appropriate dosage for the individual patient. We present two Bayesian nonlinear state space models for predicting patient leukocyte counts during the maintenance therapy. The models simplify some aspects of previously proposed models but allow for some extra flexibility. Our second model is an extension which accounts for extra variation in the leukocyte count due to a treatment adversity, infections, using C-reactive protein as a surrogate. The predictive performances of our models are compared against a model from the literature using time series cross-validation with patient data. In our experiments, our simplified models appear more robust and deliver competitive results with the model from the literature.

A Selective and Potent Oral Inhibitor of the JAK2 Tyrosine Kinase Reverses Polycythemia and Leukocytosis Induced by JAK2 V617F in a Mouse Model.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 557-557 ◽  
Author(s):  
Virginia M. Zaleskas ◽  
Wayne W. Chan ◽  
Peter Evangelista ◽  
Katherine Lazarides ◽  
Rajesh Chopra ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Myeloid Leukemia ◽  
Leukocyte Count ◽  
Peripheral Blood Leukocyte ◽  
Jak2 V617f ◽  
Myeloproliferative Disease ◽  
Short Term ◽  
Jak2 Inhibitor ◽  
Leukocyte Counts ◽  
Blood Leukocyte

Abstract A somatic mutation (V617F) in the pseudokinase domain of the non-receptor tyrosine kinase JAK2 is found in virtually all patients with the myeloproliferative disease (MPD) polycythemia vera (PV), and about half of those with essential thrombocythemia and chronic idiopathic myelofibrosis. When expressed in mice by retroviral bone marrow transduction and transplantation, JAK2 V617F but not JAK2 wild-type induces leukocytosis with neutrophilia and recapitulates the entire erythroid phenotype of PV, with polycythemia, reticulocytosis, low plasma Epo, and endogenous erythroid colonies (Zaleskas et al., PLoS ONE2006;1:e18; Wernig et al., Blood2006;107:4274; Lacout et al., Blood2006;108:1652). By analogy to BCR-ABL in chronic myeloid leukemia, JAK2 V617F is therefore a rational target for therapy in PV and other JAK2 V617F+ MPDs. Previous studies with a nonselective and parenteral tyrphostin JAK2 inhibitor suggested that short-term inhibition of JAK2 could decrease reticulocytosis in recipients of JAK2 V617F-transduced marrow (Zaleskas et al., op cit.). Here, we studied one of a novel series of inhibitors of JAK2, AZ-01, which potently inhibited JAK2 kinase activity (IC50 < 1nM) and suppressed proliferation of cell lines carrying the JAK2 V617F mutation. We treated cohorts of Balb/c mice with JAK2 V617F-induced polycythemia and leukocytosis with AZ-01 at 10 mg/kg by oral gavage twice daily, or with vehicle alone. After 28 days of treatment, AZ-01-treated mice had significant reductions in hematocrit, hemoglobin, reticulocytes, and splenomegaly, as well as normalization of peripheral blood leukocyte counts (Figure 1). These results demonstrate that oral administration of a potent and selective JAK2 inhibitor can reverse the hematopoietic abnormalities of JAK2 V617F-induced MPD in mice, and validate JAK2 as a target for therapy of human JAK2 V617F+ MPD. Figure Figure An orally available JAK2 inhibitor decreases the leukocyte count, hematocrit, and reticulocyte count in mice with JAK2 V617F-induced MPD. Leukocyte count (left), hematocrit (center) and reticulocyte counts (right) of cohorts of mice with JAK2 V617F-induced MPD treated with vehicle or drug at 10 mg/kg BID. Blood counts were assessed at 15 and 28 days of treatment. Decreases in d28 hematocrit and retic count in the drug-treated cohorts were significant (p=0.008 and p<0.0001, respectively, t-tests).

Abstract P45: White Blood Cell Counts in Aneurysmal Subarachnoid Hemorrhage Patients as a Predictor of Cardiac Injury

Stroke ◽  
2021 ◽  
Vol 52 (Suppl_1) ◽  
Author(s):  
Tiffany Cheng ◽  
Joseph R Geraghty ◽  
Neil S Saini ◽  
Yonatan Hirsch ◽  
Tyler J Lung ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Cardiac Dysfunction ◽  
Leukocyte Count ◽  
Cardiac Injury ◽  
Total Leukocyte Count ◽  
Monocyte Count ◽  
Cell Counts ◽  
Leukocyte Counts ◽  
Blood Leukocyte ◽  
The Relationship

Introduction: Up to 50% of subarachnoid hemorrhage (SAH) patients develop cardiac injury. The relationship between early systemic inflammation and cardiac injury after SAH is unknown. Here we examined changes in blood leukocyte counts and their relationship to cardiac dysfunction. Methods: We reviewed the medical records of 288 SAH patients admitted to our Comprehensive Stroke Center. Patients were dichotomized based on elevated (≥0.04ng/mL) or normal (<0.04ng/mL) troponin I (TnI). Demographics and labs from admission were then compared among the two groups by Chi-Square or Mann-Whitney test. Ejection fraction (EF) was stratified into low (<50%), normal (50-70%), or high (>70%) from echocardiogram data. We performed univariate and multivariate logistic regression to establish the relationship between blood leukocyte counts and cardiac injury. Results: Of 288 SAH patients, 241 had TnI levels performed at the time of admission and 119 (49.4%) of these had elevated TnI on admission. Patients with elevated TnI were significantly older, had higher grade SAH, abnormal EF, and were more likely to have hypertension and dyslipidemia. 10 (4.1%) had low EF while 58 (24.1%) had high EF on admission echocardiogram. In univariate analysis, total leukocyte count (p<0.0001), absolute neutrophil count (p=0.037), absolute monocyte count (p=0.014), and neutrophil-lymphocyte ratio (p=0.010) were associated with elevated TnI. Multivariate analysis adjusting for covariates showed that only total leukocyte count remained a significant predictor of elevated TnI (OR = 1.104, 95% CI= 1.020 - 1.195; p=0.014). Receiver operating characteristic (ROC) analysis demonstrated that adjusted total leukocyte count distinguishes between SAH patients with normal and elevated TnI (area under the curve = 0.787, p=0.001), with the optimal cutoff being 0.521 (sensitivity of 67.0% and specificity of 80.6%). Conclusions: Blood total leukocyte count is an independent predictor of cardiac injury in SAH patients. This highlights the role of inflammation in mediating cardiac dysfunction after brain hemorrhage, and raises questions regarding the potential of anti-inflammatory therapy for cardioprotection in SAH.

Risk of Venous Thrombosis Associated with White Cell Count On Peripheral Blood and Its Interrelationship with Other Environmental Risk Factors

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 1149-1149
Author(s):  
Suely M Rezende ◽  
Willem M. Lijfering ◽  
Frits R. Rosendaal ◽  
Suzanne C. Cannegieter
Keyword(s):  
Risk Factors ◽  
High Risk ◽  
Environmental Risk ◽  
Leukocyte Count ◽  
Risk Groups ◽  
Environmental Risk Factors ◽  
White Cell ◽  
Patients With Cancer ◽  
Leukocyte Counts ◽  
Blood Leukocyte

Abstract Abstract 1149 Background: Antithrombotic prophylaxis needs to be balanced against bleeding risk. Therefore, risk stratification is useful to identify patients who would benefit most. In this study, we analyzed how VT risk relates to different leukocyte count. We also analyzed the effect of high leukocyte counts in groups with high risk of VT. Methods: In the MEGA case-control study, blood leukocyte count and information on environmental risk factors were collected from 2443 patients with VT and 1459 partner controls. Logistic regression analyses were adjusted for age and sex. Results: Population characteristics are detailed in Table 1. Risk of VT increased for measurements above the 97.5th percentile for total white cells, granulocytes, lymphocytes and monocytes. Adjusted odds ratio, [OR] for 99th percentile was 1.37 (95% confidence interval [CI], 0.73–2.56), 1.48 (95% CI, 0.79–2.77), 1.20 (95% CI, 0.63–2.31) and 1.88 (95% CI, 1.02–3.46), for total white cells, granulocytes lymphocytes and monocytes, respectively, compared to the reference percentile (5th-95th) (Table 2). We also analysed the effect of high leukocyte counts on VT in high risk groups, including surgical patients, hospitalized patients, and patients with cancer. Adjusted OR for high leukocyte counts (white cell, lymphocyte, monocyte or granulocyte counts > 97.5th percentile) was 6.3 (95% CI, 0.84–47.1) for groups who had surgery or were hospitalized and 2.2 (95% CI, 0.6–8.2) for patients with cancer, respectively, compared with cell counts within the percentile between 5th and 97.5th. Conclusions: High blood leukocyte count is associated with increased risk of VT. The risk is further increased in high risk groups. These results may assist in individually tailored thromboprophylaxis in high risk groups. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Differential Leukocyte and Platelet Profiles in Distinct Models of Traumatic Brain Injury

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 500
Author(s):  
William Brad Hubbard ◽  
Meenakshi Banerjee ◽  
Hemendra Vekaria ◽  
Kanakanagavalli Shravani Prakhya ◽  
Smita Joshi ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Head Injury ◽  
Closed Head Injury ◽  
Diffuse Brain Injury ◽  
Blood Brain ◽  
Closed Head ◽  
Leukocyte Counts ◽  
Neutrophil Aggregation ◽  
Blood Leukocyte

Traumatic brain injury (TBI) affects over 3 million individuals every year in the U.S. There is growing appreciation that TBI can produce systemic modifications, which are in part propagated through blood–brain barrier (BBB) dysfunction and blood–brain cell interactions. As such, platelets and leukocytes contribute to mechanisms of thromboinflammation after TBI. While these mechanisms have been investigated in experimental models of contusion brain injury, less is known regarding acute alterations following mild closed head injury. To investigate the role of platelet dynamics and bioenergetics after TBI, we employed two distinct, well-established models of TBI in mice: the controlled cortical impact (CCI) model of contusion brain injury and the closed head injury (CHI) model of mild diffuse brain injury. Hematology parameters, platelet-neutrophil aggregation, and platelet respirometry were assessed acutely after injury. CCI resulted in an early drop in blood leukocyte counts, while CHI increased blood leukocyte counts early after injury. Platelet-neutrophil aggregation was altered acutely after CCI compared to sham. Furthermore, platelet bioenergetic coupling efficiency was transiently reduced at 6 h and increased at 24 h post-CCI. After CHI, oxidative phosphorylation in intact platelets was reduced at 6 h and increased at 24 h compared to sham. Taken together, these data demonstrate that brain trauma initiates alterations in platelet-leukocyte dynamics and platelet metabolism, which may be time- and injury-dependent, providing evidence that platelets carry a peripheral signature of brain injury. The unique trend of platelet bioenergetics after two distinct types of TBI suggests the potential for utilization in prognosis.

scholarly journals THE EFFECT OF ADMINISTRATION OF EPINEPHRINE ON THE LEUKOCYTE COUNTS OF NORMAL SUBJECTS

Blood ◽  
1950 ◽  
Vol 5 (8) ◽  
pp. 723-731 ◽  
Author(s):  
COLIN WHITE ◽  
TSUIN HWA LING ◽  
ARNOLD M. KLEIN
Keyword(s):  
Eosinophil Count ◽  
Adrenocorticotropic Hormone ◽  
Leukocyte Count ◽  
Normal Subjects ◽  
Total Count ◽  
Subcutaneous Injections ◽  
History Of ◽  
Leukocyte Counts ◽  
Adrenocortical Hormone ◽  
The One

Abstract 1. Thirty-seven normal subjects were given subcutaneous injections of epinephrine, ranging from 0.25 to 0.5 mg., and the effects on the leukocytes were noted. 2. The neutrophils rose steadily for the three and one-half hours during which blood counts were made. The small lymphocytes rose in the first half hour, then fell below normal and finally returned towards normal. The eosinophils rose at first and then fell below normal for the remainder of the period. 3. The three doses of epinephrine used produced effects which differed quantitatively but not qualitatively. The most readily identified effect of the smallest dose was the one-half hour rise in lymphocytes or the one-half hour rise in total count. A dose of 0.5 mg. is satisfactory for work of this kind. 4. Subjects with a history of allergy showed a greater tendency than the remainder to exhibit a one-half hour rise in the eosinophil count. 5. The changes in the leukocyte count produced by epinephrine are similar to, but not identical with, those produced by adrenocortical hormone or adrenocorticotropic hormone.

Analysis of Support for Parents in Raising Children with Dyslexia

2020 ◽  
pp. 0192513X2094892
Author(s):  
Athira Amira Abd Rauf ◽  
Maizatul Akmar Ismail ◽  
Vimala Balakrishnan ◽  
Loh Sau Cheong ◽  
Novia Indriaty Admodisastro ◽  
...  
Keyword(s):  
Marital Status ◽  
Self Esteem ◽  
Peer To Peer ◽  
Government Support ◽  
Anova Analysis ◽  
Raising Children ◽  
The Social ◽  
Significant Difference ◽  
Different Types ◽  
One Way Anova

The parents of children with dyslexia often experience more parenting stress and depressive symptoms compared to other parents. Studies have shown that supporting and encouraging such parents help in reducing their frustrations, fear, anger, and low self-esteem towards their children. The purpose of this study was to identify and examine the different types of support needed by the parents of children with dyslexia and to explore the relationships between the required support with the parents’ marital status. Fifty questionnaires were distributed to the parents of children with dyslexia and analyzed. The findings showed that the type of support needed for parents of children with dyslexia could be grouped into social, peer-to-peer, financial, and government support. Furthermore, the analysis indicated that there were no significant differences between the social (p = 0.4014), peer-to-peer (p = 0.5020), and government (p = 0.7121) support with parents’ marital status. However, based on one-way ANOVA analysis, there was a significant difference found between the parents’ marital status and financial support (p = 0.0241). Accordingly, it is anticipated that the implication of this research could be used as a guide and a reference for supporting and encouraging parents of children with dyslexia and other learning disabilities.

Blood leukocyte count is a risk factor for intima-media thickening and subclinical carotid atherosclerosis in middle-aged men

2006 ◽  
Vol 188 (2) ◽  
pp. 363-369 ◽  
Author(s):  
Antti Loimaala ◽  
Riikka Rontu ◽  
Ilkka Vuori ◽  
Michele Mercuri ◽  
Terho Lehtimäki ◽  
...  
Keyword(s):  
Risk Factor ◽  
Carotid Atherosclerosis ◽  
Leukocyte Count ◽  
Middle Aged ◽  
Blood Leukocyte

scholarly journals PENGARUH FASE BULAN TERHADAP WAKTU TEBAR PANCING DAN LAJU TANGKAP MADIDIHANG (Thunnus albacares Bonnaterre, 1788) PADA ARMADA RAWAI TUNA

2017 ◽  
Vol 22 (4) ◽  
pp. 207
Author(s):  
Irwan Jatmiko ◽  
Bram Setyadji ◽  
Arief Wujdi
Keyword(s):  
Full Moon ◽  
Yellowfin Tuna ◽  
Catch Rate ◽  
Lunar Phase ◽  
Anova Analysis ◽  
Tukey Test ◽  
Significant Difference ◽  
New Moon ◽  
One Way Anova ◽  
Tuna Longline

Madidihang/yellowfin tuna merupakan salah satu jenis ikan tuna ekonomis penting bagi industri perikanan di Indonesia dengan kontribusi hasil tangkapan yang terbanyak. Penelitian ini bertujuan untuk mengetahui pengaruh fase bulan terhadap waktu mulai tebar pancing dan laju tangkap madidihang pada armada rawai tuna. Pengumpulan data dilakukan oleh pemantau ilmiah pada armada rawai tuna yang sebagian besar berbasis di Pelabuhan Benoa, Bali mulai Agustus 2005 hingga Juni 2014. Daerah penangkapan ikan dari armada rawai tuna yang diambil datanya berada di lokasi (lintang dan bujur) 9°-16° LS hingga 109°-120° BT. Analisis anova satu arah dan tes Tukey dilakukan untuk mengetahui pengaruh fase bulan terhadap waktu mulai tebar pancing dan laju tangkap madidihang. Total sebanyak 60 trip dan 1.467 hari operasi penangkapan armada rawai tuna dilakukan dalam penelitian ini. Analisis statistik anova satu arah menunjukkan bahwa terdapat pengaruh yang nyata pada fase bulan terhadap waktu mulai tebar pancing (p<0,05). Selanjutnya, tes Tukey menunjukkan bahwa waktu mulai tebar pancing pada saat bulan purnama dimulai pada pukul 9:00 pagi hari. Waktu ini lebih lambat sekitar 2 jam dari pada waktu mulai tebar pancing pada ketiga fase bulan lainnya (perbani awal, perbani akhir dan bulan baru) yang dilakukan sekitar pukul 7:00 pagi hari. Analisis statistik anova satu arah juga menunjukkan terdapat perbedaan yang nyata antar fase bulan terhadap laju tangkap madidihang (p<0,05). Selanjutnya, tes Tukey menunjukkan bahwa laju tangkap pada saat bulan baru dan perbani awal sebesar 0,13 ekor/100 mata pancing atau lebih besar dibandingkan nilai laju tangkap pada saat purnama dan perbani akhir yang hanya sebesar 0,09 ekor/100 mata pancing. Yellowfin tuna is one of the most economically important species for fisheries industry in Indonesia. The objectives of this study are to investigate the effect of lunar phase to the set time start and catch rate of yellowfin tuna on tuna longline vessels. Data collected by scientific observer on tuna longline vessels mainly based in Benoa Port, Bali from August 2005 to June 2014. Fishing ground of sampled longline tuna located from 9°-16° S to 109°-120° E. One-way anova analysis and Tukey test conducted to examine the effect of lunar phase to the set time start and catch rate of yellowfin tuna. A total of 60 trips and 1,467 fishing days of longline tuna fishing vessels operation have been sampled for in this study. One-way anova analysis showed that there was a significant difference of lunar phase to the set time start (p<0.05). Furthermore, Tukey test showed that the starting time for setting during the full moon begins at 9:00 am. Its time was around 2 hours slower than the start of setting of the other three moon phase (first quarter, last quarter and new moon) which start around 7:00 am. One-way anova analysis also showed that there was a significant difference of lunar phase to catch rate of yellowfin tuna (p<0.05). Furthermore, Tukey test confirmed that hook rate on new moon and first quarter was 0.13/100 hooks or 0.4 bigger than hook rate on full moon and last quarter with only 0.09/100 hooks.

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