scholarly journals Topical Clover Flower Honey Administration Accelerated Wound Healing in Swiss Webster Mice

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Hans Kristian ◽  
Iwan Budiman ◽  
Stella T Hasianna
Keyword(s):  
Wound Healing ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Wound Treatment ◽  
Collagen Density ◽  
Incision Wound ◽  
Fibroblast Density ◽  
Wound Healing Activity

 AbstractClover flower honey is a herbal therapy used as an alternative wound treatment. The objective of this study was to determine the effect of Clover flower honey in accelerating incision wound healing, enhancing reepithelization degree, increasing fibroblast density, and collagen. This was a true experimental research, using 25 mice divided into 5 groups (n=5); positive control group (feracrylum 1%), negative control group (aquadest), 100% honey group, 50% honey group, and 25% honey group. Wound healing activity was measured macroscopically with calipers, dan microscopically through pathological anatomy preparations to observe reepithelization degree and collagen density. This study showed wound incision lengths were different significantly between Clover flower honey 100%, 50%, and 25% compared to aquadest (p ≤ 0.05), and feracrylum 1% (p ≤ 0.05). Reepithelization degree, fibroblast and collagen density measurements between Clover flower honey 100%, 50%, and 25% were different significantly compared with aquadest (p ≤ 0.05), and only the 100% honey group was different significantly with feracrylum 1% (p ≤ 0.05). We concluded topical Clover flower honey administration accelerated wound healing, increased reepithelization degree, fibroblast density, and collagen degree.Keywords : wound healing, reepithelization degree, fibroblast density, collagen density, Clover flower honey

Effects of 1% and 3% Mobe Leaf Extract Gel on Socket Wound Healing after Tooth Extraction

2021 ◽  
Vol 24 (1) ◽  
pp. 1-5
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Denny Satria ◽  
Avi Syafitri
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Gel Treatment

Tooth extraction is a process of removing teeth from the alveolar bone. In wound healing, fibroblast are very important cells. The main purpose of this study was to determine the effect of mobe leaf 1% and 3% extract gel (Artocarpus lakoocha) on fibroblast proliferation in post extraction tooth socket wound healing. This research used 16 samples of wistar rats, divided into 4 groups, a positive control group, a negative control group and a 1% and 3% mobe leaf extract gel group. The left mandibular incisors were extracted, then 1% and 3% gels of mobe leaf extract were applied on day 1 to day 7. Data analysis was calculated using the Kruskal-Wallis test on clinical data and one way ANOVA test for microscopic. The result of the socket wound healing activity test for a good concentration of mobe leaf extract gel was 3%. This research shows significant resultith p-value of 0.018 (< 0.05) on the closure of the socket wound clinically which means the closure of the wound accelerates because of the mobe leaf 3% extract gel treatment. The distance of fibroblast on microscopically shows significant resultith a p-value of 0.002 (< 0.05), which means that there was an enlargement of the distance fibroblast at the socket wound closure with application of mobe leaf 3% extract gel. From the results of the study it can be concluded that mobe leaf 3% extract gel has the best ability to show acceleration the closure of the socket wound either clinically or microscopically.

scholarly journals Percepatan Penutupan Luka Sayat pada Tikus Putih Akibat Pemberian Perasan Rimpang Temulawak (Curcuma xanthorrhiza Roxb.)

2021 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Elisabeth Natalia Barung ◽  
Rifny Wungow ◽  
Donald Emilio Kalonio
Keyword(s):  
Wound Healing ◽  
Linear Regression Analysis ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
White Rats ◽  
Curcuma Xanthorrhiza ◽  
Incision Wound

Rimpang Temulawak atau Curcuma xanthorriza Roxb. adalah tanaman yang dikenal luas oleh masyarakat sebagai obat tradisional. Rimpang temulawak mengandung kurkumin dan xanthorrizzol, yang diketahui mampu mempercepat penutupan luka di kulit dan juga memiliki efek antibakteri dan antiinflamasi. Penelitian ini bertujuan untuk untuk mengetahui efektifitas perasan rimpang temulawak terhadap percepatan penutupan luka sayat pada tikus putih. Penelitian ini adalah penelitian eksperimen, dengan subyek 10 ekor tikus putih yang dibagi dalam 2 kelompok perlakuan yaitu kelompok yang diberi perasan temulawak dan kelompok kontrol negatif yang tidak diberi perlakuan. Data dikumpulkan dengan mengukur panjang luka tikus menggunakan alat ukur penggaris dan dihitung persentasi penutupan luka. Data dianalisis menggunakan analisis regresi linear dan nilai slope (b) dinyatakan sebagai kecepatan penutupan luka. Hasil penelitian ini, menunjukan bahwa rimpang temulawak mampu mempercepat penutupan luka sayat sebesar 15,262%/hari dibandingkan kelompok yang tidak diberi perlakuan sebesar 13,54%/hari. Kata Kunci: Perasan Rimpang Temulawak, Luka Sayat, Percepatan Penutupan Luka, Sediaan Sederhana, Obat Tradisional Indonesia Curcuma xanthorrhiza Roxb. is a plant that is widely known by the community as traditional medicine. The rhizome of C. xanthorrhiza contains curcumin and xanthorrhizol, which are known to be able to accelerate wound healing on the skin and also has antibacterial and anti-inflammatory effects. This study was aimed to determine the effectiveness of C. xanthorriza rhizome on the acceleration of incision wound healing on white rats. This study was an experimental study, with 10 white rat subjects divided into two treatment groups, namely the treatment group which was given by C. xanthorriza rhizome and the negative control group that was not treated. Data were collected by measuring rat wound length using a ruler and calculating the percentage of wound healing. By using linear regression analysis and the value of the slope (b) is expressed as the acceleration of wound healing. The results of the study showed that C. xanthorrhiza rhizome was able to accelerate incision wound healing by 15.262% / day compared to the untreated group of 13.54% / day.

scholarly journals Evaluation of the Wound Healing Activity of the Crude Extract of Leaves of Acanthus polystachyus Delile (Acanthaceae)

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Wubante Demilew ◽  
Getnet Mequanint Adinew ◽  
Seyfe Asrade
Keyword(s):  
Wound Healing ◽  
Medicinal Plants ◽  
Crude Extract ◽  
Negative Control Group ◽  
Treated Group ◽  
Negative Control ◽  
Control Group ◽  
Wound Model ◽  
Treatment Of Wounds ◽  
Wound Healing Activity

Background. Medicinal plants play indispensable roles to treat various ailments. Acanthus polystachyus is one of the medicinal plants used traditionally for treatment of wounds. However, there were no scientific reports documented so far on the wound healing activities of this plant. Thus, the present study provides a scientific evaluation for the wound healing potential of the crude extract of A. polystachyus leaves. Methods. The crude extraction was carried out using 80% methanol. The crude extract was prepared in 5% (w/w) and 10% (w/w) ointment and evaluated for wound healing activity using excision, infected, and incision wound models in Swiss albino mice. Results. Both 5%  and 10% (w/w) ointments significantly reduced period of epithelialization and increased wound contraction rate and tensile strength compared to the negative control group (P < 0.05). The wound healing activity of 10% (w/w) ointment treated group was greater than 5% (w/w) and nitrofurazone ointment treated groups in S. aureus infected wound model. Conclusion. These results demonstrate that the crude extract of A. polystachyus leaves possesses wound healing activities. This justifies the traditional claimed use of the plant for treating uninfected and infected wounds caused by S. aureus.

scholarly journals Efek Pemberian Ekstrak Daun Pegagan (Centella Asiatica) Terhadap Penyembuhan Luka Sayat Pada Tikus Putih Jantan (Rattus Norvegicus) Galur Wistar

2020 ◽  
Vol 9 (1) ◽  
pp. 21
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain

Wound refers to partly damage or loss of body tissue. One way that has been used by the people in helping the process of wound healing is the use of medicinal plants, such as gotu kola (Centella asiatica). Gotu kola (Centella asiatica)  is kind of plant that found almost in all territories of Indonesia which functions as a cell revitalizing substance which quickens wound healing those are burnt, skin ulcer and to prevent keloid. This study is aimed to know the effect of giving gotu kola leaf extract (Centella Asiatica) against to the healing of the wound incision on male white rats (Rattus Norvegicus) wistar strain. This study is a pure experimental research using the randomized post test only control group design with the subjects are 30 male white rats (Rattus norvegicus) wistar strain that were divided into 3 groups, namely K1, K2, and K3. The incision wound were made on the back of all white rat in each group with a length of ± 2 cm and a depth of ± 2 mm. The wound in the negative control group (K1) was not given any substance; in the positive control group (K2) was given povidone iodine 10%; and in the treatment group (K3) was given gotu kola leaf extract at a dose of 37,5 mg/150 grBB. The study was done for 10 days. Furthermore, wound observation and monitoring is held. Then the results were processed by using One Way ANOVA. The result study of wound incision healing on male white rats (Rattus norvegicus) wistar strain shows that there is significant difference among the negative control group (K1), the positive control group (K2), and the treatment group (K3) on One Way ANOVA test which significant p-value are 0,027 (p < 0.05). The result of Post-Hoc LSD test also shows a significant differences among the K1 and K3 groups, and K2 and K3 groups which significant p-values are 0,011 and 0,038 (p < 0,05). From these results, it can be concluded that gotu kola leaf extract (Centella asiatica) effective on quickens wound healing of the wound incision on male white rats (Rattus norvegicus) wistar strain.

scholarly journals Extract effect of Tintir (Jathropa Multifida Linn) to Total PMN and Selt Mast in Wound Healing Model Rats with Acute Injury

2019 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
Wahyuningsih Wahyuningsih
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Significant Difference ◽  
Pmn Leukocytes ◽  
Stem Extract

Wound is considered as a serious health problem, because it can interfere with quality of life due to pain, weakness, and decreased mobility and quality of life. This study aims to determine the effect ofj jathropha multifida, linn to the number of PMN and selt mast in the process of wound healing in rats with acute injury models. This study is an experimental research laboratory using the design of post test control group design Wistar rats were used as research subjects. Based on Tukey HSD test results, the average number of PMN leukocytes third day the negative control group had no significant difference in the positive control group (0.037) and treatment group (0004). On the seventh day the number of PMN leukocytes negative control group had no significant difference in the positive control group (0.069) and had a significant difference to the treatment group (0.00). Meanwhile, on the fourteenth day the number of PMN leukocytes negative control group had no significant difference in the positive control group (0.000) and had a significant difference to the treatment group (0.00). Based on the results of data analysis Annova one direction on the third day obtained a significant level of 0:50 means there is no significant difference in the number of mast cells in each group on the seventh day. While on day three significant values ​​of 0:08 was also no significant difference, as well as on the fourteenth day found no significant difference between the group with a value of 0.68. Conclusions there was a decrease in the number of Leukocyte PMN in rat wounds given a 10% stem extract ointment, and there was a decrease in the number of mast cells in the wounds of rodents given a 10% stem extract ointment. Suggestions need to be investigated regarding the use of distance stem extract in various concentrations to obtain effective results for wound healing and the need to examine other factors related to wound healing.

scholarly journals PENGARUH PEMBERIAN EKSTRAK PROPOLIS LEBAH TANPA SENGAT PADA PENYEMBUHAN LUKA BAKAR TIKUS PUTIH (Rattus norvegicus)

2021 ◽  
Vol 8 (1) ◽  
pp. 68-76
Author(s):  
Nelky Suriawanto ◽  
Evi Setyawati ◽  
Narwan
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Stingless Bee ◽  
Control Group ◽  
Burn Wound ◽  
Propolis Extract ◽  
Burn Wound Healing

The Effect of Treatment Using Stingless Bee Propolis Extract on Burn Wound Healing in Rattus norvegicus Burn would occurs as a result of direct or indirect fire burns, exposure to sunlight and chemicals. This study aims to analyze the effect of stingless bee propolis extract (Tetragonula fuscobalteata) on the burn wound healing of the back skin of Rattus norvegicus. An experimental research was carried out using the control and experimental groups. Twenty five R. norvegicus were divided into 5 groups: the negative control group was given 70% ethanol, the positive control group was given Bioplacenton® and 3 groups were treated with propolis extract with a concentration of 50%, 70% and 100%. Measurement of burn diameter was carried out every 2 days for 21 days. Data were analyzed statistically using the one way Anova method. The results of phytochemical screening showed that the stingless bee propolis extract contained flavonoids, phenolics, tannins and saponins. The test of the effectiveness of propolis extract against burn wound obtained a sig value of 0.00 (< 0.05). stingless bee propolis extract could heal burn wound in R. norvegicus. Luka bakar dapat terjadi akibat terbakar api secara langsung atau tidak langsung, paparan sinar matahari dan bahan kimia. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak propolis lebah tanpa sengat (Tetragonula fuscobalteata) terhadap penyembuhan luka bakar pada kulit punggung Rattus norvegicus. Metode yang digunakan merupakan penelitian true experimental menggunakan kelompok kontrol dan eksperimen. Dua puluh lima ekor R. norvegicus dibagi menjadi 5 kelompok: kelompok kontrol negatif diberi etanol 70%, kontrol positif diberi Bioplacenton®, dan 3 kelompok perlakuan ekstrak propolis dengan konsentrasi 50%, 70%, dan 100%. Pengukuran diameter luka bakar dilakukan setiap 2 hari selama 21 hari. Data dianalisa secara statistik menggunakan metode Anova satu arah. Hasil skrining fitokimia ekstrak propolis lebah tanpa sengat positif mengandung senyawa flavonoid, fenolik, tanin, dan saponin. Uji efektivitas ekstrak propolis terhadap luka bakar didapatkan nilai sig 0,00 (< 0,05). Ekstrak propolis lebah tanpa sengat dapat menyembuhkan luka bakar pada R. norvegicus.

scholarly journals Is traditional herbal mix composition effective on wound healing?

2021 ◽  
Vol 8 (1) ◽  
pp. 29-38
Author(s):  
Serap Gokce Eskin ◽  
Yucel Basimoglu Koca ◽  
Serdal Ogut
Keyword(s):  
Wound Healing ◽  
Negative Control Group ◽  
Grape Seed ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Wound Model ◽  
Experimental Group

Objective: Wound healing is a dynamic process that includes biochemical and physiological phenomena. Studies confirm that plants are used in wound healing to a great extent.  Antique oil (HBX 2371) has been produced as a traditional herbal mixed. This herbal mixture contains sesame oil, thyme oil, olive leaf, fig seed, grape seed, turmeric, and cinnamon. We evaluated traditional plant mixed oil ( Antique oil) for its wound-healing activity using an excision wound model in rats. Methods: The study used a preclinical, in vivo experimental, and analytical design. In the study, a total of 21 Sprague-Dawley rats, including 7 as experimental group, 7 as negative-control group, and 7 as positive-control group, were used. After the ischemic wound was created in experimental animals, the research data relating to the histochemical changes and biochemical parameters of the wound healing parameters were collected Results: A to research in experimental group fibroblast count, collagen density, fat cells, epithelization scores higher than the other groups and inflammatory cell density lower than the others. According to the results of the study, the highest TAC value (3.94 ± 0.21) was determined in the experimental group and results showed that antique oil administration decreased the TOC value. Conclusion: In conclusion, it was observed that antique oil prevented the wound changes induced by the incision, increased the repair of the epidermal and dermal structure in a short time, increased the antioxidant level, and decreased the oxidation level. Clinical studies are recommended.

scholarly journals SGOT and SGPT level of Wistar rat after the administration of Channa micropeltes extract

2020 ◽  
Vol 11 (4) ◽  
pp. 5415-5421
Author(s):  
Amy Nindia Carabelly ◽  
Deby Kania Tri Putri ◽  
Nadya Rezki ◽  
Maharani Laillyza Apriasari
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Oral Administration ◽  
Rat Liver ◽  
Wistar Rat ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Channa Striata

Channa micropeltes is a local peatland water species which has been utilized as an alternative of Channa striata among South Kalimantan population to anticipate the scarcity of Haruan species. Channa micropeltes originate from the same family of Channa striata in which both fishes contain albumin. Albumin is proven to accelerate wound healing, thus can be produced as capsules. The capsulate form of Channa micropeltes extract will later be deployed as an alternative herbal medicine for the acceleration of mucosal wound healing. Nevertheless, a study of a hepatological profile is pivotal to assess the safety of its consumption. The present study aimed to analyze the effect of Channa micropeltes extracts capsulate oral administration at 0.7 g dosage on SGOT and SGPT level of Wistar rat liver. This was a real experimental study with post-test only and control group design. The samples comprised of 12 rats which were distributed into three groups namely a treatment group of 0.7 g Channa extract capsulate, a positive control group of 0.7 g Channa striata extract and a negative control group without any treatment for 28 days. The level of SGOT and SGPT were 19.41 IU/L and 29.52 IU/L in Channa micropeltes treatment group, 31.95 IU/L and 28.71 IU/L in the positive control group of 0.7 g Channa striata extract, 25.07 IU/L and 18.90 IU/L in the negative control group. Hence, there is no effect of Channa micropeltes extract capsulate oral administration at 0.7 g dosage on SGOT and SGPT level of Wistar rat liver.

scholarly journals The effects of shark liver oil on fibroblasts and collagen density in the periodontal ligaments of Wistar rats induced with Porphyromonas gingivalis

2019 ◽  
Vol 52 (4) ◽  
pp. 209
Author(s):  
Dian Mulawarmanti ◽  
Dwi Andriani ◽  
Dian Widya Damaiyanti ◽  
Farizia Putri Khoirunnisa ◽  
Alifati Nita Juliatin
Keyword(s):  
Periodontal Ligament ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Treatment Groups ◽  
Shark Liver Oil ◽  
Collagen Density

Background: Periodontitis is an infection in tooth-supporting tissues caused by a specific microorganism, Porphyromonas Gingivalis (PG), which can trigger collagen destruction. Generally, periodontal therapy employs a combination of mechanical (scaling root planning/SRP) and chemical (antibiotics) remedies, the latter of which can cause bacterial resistance. On the other hand, shark liver oil contains active natural ingredients such as alkylglycerols, squalene, squalamine, and omega-3, which have antibacterial and antioxidant effects. Purpose: This study aims to determine the impact of shark liver oil on fibroblasts and collagen density in the periodontal ligament of Wistar rats induced with PG. Methods: This study represents a laboratory experiment with post-test only control group design. The research subjects consisted of 35 Wistar rats divided into five groups, namely; a negative control group (K-); a positive control group with PG induction (K+); and three treatment groups induced with PG and shark liver oil once a day for seven days at varying doses of 0.2 g/gBB (P1), 0.3 g/gBB (P2), and 0.4 g/gBB (P3). Following treatment, the subjects were euthanized. The number of fibroblasts was then histologically examined with Hematoxylin Eosin (HE). Meanwhile, the collagen density was histologically analyzed with Masson’s Tricrome. Fibroblast cells were observed through a microscope at 400x magnification. Data was statistically analyzed with a one-way ANOVA and post hoc LSD. Collagen Density scoring was then performed. The results were analyzed with a non-parametric Kruskal-Wallis test (p=0.05), and subsequently with a Mann-Whitney U test (p<0.05). Results: The number of fibroblasts in the periodontal ligament areas of each group were 18.6 ± 1.21 for K-; 12 ± 1.26 for K;16.8 ± 1.72 for P1; 17.1 ± 1.94 for P2; and 23.16 ± 2.78 for P3. The results also indicated that there were significant differences between K- with K+ and P3, K+ with P1, P2, and P3, as well as P3 with P1 and P2. However, there was no significant difference between K- and P1 and P2 or P1 and P2. The results showed that collagen density in the negative control group did not significantly decrease compared to that in the positive control group in which PG was induced. Meanwhile, collagen density in all three treatment groups following doses of 0.2 g/gBB, 0.3 g/gBB, and 0.4 g/gBB being administered significantly increased compared to that in the negative control group and the positive control group subjected to PG induction. Conclusion: Shark liver oil can significantly increase fibroblast cells and collagen density in the periodontal ligament of Wistar rats induced with PG.

scholarly journals The Influence of Channa Striata Extract Emulgel on Incision Wound Healing in White Rats

2019 ◽  
Vol 24 (3) ◽  
pp. 210
Author(s):  
Lucia Hendriati ◽  
Suryo Kuncorojakti ◽  
Teguh Widodo ◽  
Heny Kristi Meitasari ◽  
Wilis Prasasti
Keyword(s):  
Wound Healing ◽  
Positive Control ◽  
Negative Control ◽  
White Rats ◽  
Length Increase ◽  
Albumin Content ◽  
Channa Striata ◽  
Collagen Density ◽  
Wound Length ◽  
Incision Wound

Snakehead fish (Channa striata) have high albumin content, a protein needed for cell development and the formation of new tissue. The aim of this study was to determine the influence of snakehead fish extract emulgel given topically on incision wounds in white rats. The parameters of wound healing consist of wound length, a number of neutrophils, macrophages, fibroblasts and density of collagen. The white rats divide into three groups of (n = 6), one group was given the emulgel base as the negative control, one group of povidone-iodine as the positive control, and one group of snakehead fish extract 10% emulgel. White rats were sacrificed on the third and seventh days for microscopic observations. The results showed that snakehead fish extract emulgel can accelerate incision wound healing: decrease wound length, increase the number of neutrophil and macrophages cells, increase the average number of fibroblasts and increase collagen density on white rats.

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