scholarly journals Bioactivity Determination of Recombinant lysostaphin Immobilized on Glass Surfaces Modified by Cold Atmospheric Plasma on Staphylococcus aureus

2019 ◽  
Vol 7 (4) ◽  
pp. 132-137
Author(s):  
Gelareh Ehsani ◽  
Foad Fahmide ◽  
Dariush Norouzian ◽  
Seyed Mohammad Atyabi ◽  
Parastoo Ehsani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma ◽  
Glass Surfaces

In vitro susceptibility of methicillin-resistant and methicillin-susceptible strains of Staphylococcus aureus to two different cold atmospheric plasma sources

Infection ◽  
2016 ◽  
Vol 44 (4) ◽  
pp. 531-537 ◽  
Author(s):  
Matthias Napp ◽  
Georg Daeschlein ◽  
Sebastian von Podewils ◽  
Peter Hinz ◽  
Steffen Emmert ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Atmospheric Plasma ◽  
In Vitro Susceptibility ◽  
Methicillin Resistant ◽  
Plasma Sources ◽  
Cold Atmospheric Plasma

scholarly journals Cold Atmospheric Plasma Promotes Killing of Staphylococcus aureus by Macrophages

mSphere ◽  
2021 ◽  
Author(s):  
Constance Duchesne ◽  
Nadira Frescaline ◽  
Océane Blaise ◽  
Jean-Jacques Lataillade ◽  
Sébastien Banzet ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Soft Tissue ◽  
Atmospheric Plasma ◽  
Soft Tissue Infections ◽  
Content Type ◽  
Cold Atmospheric Plasma ◽  
Resistant Strains

Staphylococcus aureus is the most frequent cause of skin and soft tissue infections. Treatment failures are increasingly common due to antibiotic resistance and the emergence of resistant strains.

Sub-lethal exposure to cold atmospheric plasma in vitro induces changes in bacterial antibiotic resistance profiles. A pilot study.

2019 ◽  
Vol 16 (1) ◽  
Author(s):  
Melina E. Martínez-Barrera ◽  
Jaime Bustos Martinez ◽  
Leonor Sánchez-Pérez ◽  
Aída Hamdan-Partida ◽  
A Enrique Acosta-Gio
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma ◽  
Bacterial Antibiotic Resistance ◽  
Exposure To Cold

To evaluate the effect of sub-lethal exposure to cold atmospheric plasma (CAP) on their antibiotic resistance, Methicillin Resistant Staphylococcus aureus, S. epidermidis, Pseudomonas aeruginosa, Escherichia coli, Streptococcus mutans, and Candida albicans were exposed in vitro to a commercially available CAP. This antimicrobial CAP inhibited growth but changed survivors’ antibiotic resistance.

Determination of the Efficacy of the Cold Atmospheric Plasma with Nano Tio2 Covered in Cathode Towards Enveloped Viruses such as Covid 19 and Influenza in Room Air

2021 ◽  
Vol 15 (5) ◽  
pp. 1656-1660
Author(s):  
Mehrdad Fojlaley ◽  
Berat Guvenc Aslan
Keyword(s):  
No Reduction ◽  
Atmospheric Plasma ◽  
Viral Titre ◽  
Nano Tio2 ◽  
Enveloped Viruses ◽  
Air Samples ◽  
Cold Atmospheric Plasma ◽  
Log Reduction ◽  
Total Test

In this research, Medwave air and surface disinfection system (model:Klin20)selected to investigation effect , towards aerosolised enveloped viruses in room air. Phi6Pseudomonas syringae phage, a surrogate for coronavirus and influenza, was used in the trials Viral suspensions of Phi6 were aerosolised within the ukas accreditation Campden BRI aerobiology laboratory to achieve initial levels of ~106PFU/m3, representing very heavily contaminated air . Air samples were taken at 15 minute intervals and analysed to determine levels of Phi6 in the room air over a total test period of 135 minutes. On 3 separate days, paired trials were carried out with the Medwave switched onand with the units witched off as a control to determine baseline levels of virus in the air overtime. Trials carried out on the first two test days showed no reduction in viral titre compared with the control. Further investigation revealed that a wiring loom within the test unit had become disconnected during transport and the instrument was therefore not functioning correctly. Results from the trial showed that the level of Phi6 in the room air decreased rapidly from an initial titre of 6.12 log PFU/m3to undetectable levels (<1.78 log PFU/m3) after 45 minutes of operation, representing a log reduction of ≥4.00 logs compared with the control run with the unit switched off. Log reductions of 2.21, 3.30and ≥4.00 logs were observed after 15, 30 and 45 minutes respective to the log PFU/m3countsin the control run Keywords: Bioaerosols nano-titanium atmospheric cold plasma, covid19

Determination of the optimum conditions for lung cancer cells treatment using cold atmospheric plasma

2016 ◽  
Vol 23 (10) ◽  
pp. 103512 ◽  
Author(s):  
Morteza Akhlaghi ◽  
Hajar Rajaei ◽  
Amir Shahriar Mashayekh ◽  
Mojtaba Shafiae ◽  
Hamed Mahdikia ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Atmospheric Plasma ◽  
Lung Cancer Cells ◽  
Optimum Conditions ◽  
Cold Atmospheric Plasma

scholarly journals Protective Effect of the Golden Staphyloxanthin Biosynthesis Pathway on Staphylococcus aureus under Cold Atmospheric Plasma Treatment

2019 ◽  
Vol 86 (3) ◽  
Author(s):  
Yi Yang ◽  
Hao Wang ◽  
Huyue Zhou ◽  
Zhen Hu ◽  
Weilong Shang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biosynthetic Pathway ◽  
Microbial Inactivation ◽  
Atmospheric Plasma ◽  
Wild Type ◽  
Content Type ◽  
Cold Atmospheric Plasma ◽  
Promising Strategy ◽  
Bactericidal Effects ◽  
Microorganism Inactivation

ABSTRACT Staphylococcus aureus infection poses a serious threat to public health, and antibiotic resistance has complicated the clinical treatment and limited the solutions available to solve this problem. Cold atmospheric plasma (CAP) is a promising strategy for microorganism inactivation. However, the mechanisms of microbial inactivation or resistance remain unclear. In this study, we treated S. aureus strains with a self-assembled CAP device and found that CAP can kill S. aureus in an exposure time-dependent manner. In addition, the liquid environment can influence the survival rate of S. aureus post-CAP treatment. The S. aureus cells can be completely inactivated in normal saline and phosphate-buffered saline but not in tryptic soy broth culture medium. Scanning and transmission electron microscopy revealed that the CAP-treated S. aureus cells maintained integrated morphological structures, similar to the wild-type strain. Importantly, the CAP-treated S. aureus cells exhibited a reduced pigment phenotype. Deletion of the staphyloxanthin biosynthetic genes crtM and crtN deprived the pigmentation ability of S. aureus Newman. Both the Newman-ΔcrtM and Newman-ΔcrtN mutants presented high sensitivity to CAP treatment, whereas Newman-ΔcrtO exhibited a survival rate comparable to wild-type Newman after CAP treatment. Our data demonstrated that the yellow pigment intermediates of the staphyloxanthin biosynthetic pathway are responsible for the protection of S. aureus from CAP inactivation. The key enzymes, such as CrtM and CrtN, of the golden staphyloxanthin biosynthetic pathway could be important targets for the design of novel sterilization strategies against S. aureus infections. IMPORTANCE Staphylococcus aureus is an important pathogen that can be widely distributed in the community and clinical settings. The emergence of S. aureus with multiple-antibiotic resistance has complicated staphylococcal infection control. The development of alternative strategies with powerful bactericidal effects is urgently needed. Cold atmospheric plasma (CAP) is a promising strategy for microorganism inactivation. Nevertheless, the underlying mechanisms of microbial inactivation or resistance are not completely illustrated. In this study, we validated the bactericidal effects of CAP on S. aureus, including antibiotic-resistant strains. We also found that the golden staphyloxanthin, as well as its yellow pigment intermediates, protected S. aureus against CAP, and blocking the staphyloxanthin synthesis pathway at the early steps could strengthen the sensitivity of S. aureus to CAP treatment. These data provide insights into the germicidal mechanism of CAP from the aspect of bacteria and suggest new targets against S. aureus infections.

Targeting Protective Catalase of Tumor Cells with Cold Atmospheric Plasma- Activated Medium (PAM)

2018 ◽  
Vol 18 (6) ◽  
pp. 784-804 ◽  
Author(s):  
Georg Bauer
Keyword(s):  
Control System ◽  
Singlet Oxygen ◽  
Tumor Cells ◽  
Plasma Potential ◽  
Atmospheric Plasma ◽  
Antitumor Action ◽  
Active Principle ◽  
Reaction Products ◽  
Cold Atmospheric Plasma

Background: Application of cold atmospheric plasma to medium generates “plasma-activated medium” that induces apoptosis selectively in tumor cells and that has an antitumor effect in vivo. The underlying mechanisms are not well understood. Objective: Elucidation of potential chemical interactions within plasma-activated medium and of reactions of medium components with specific target structures of tumor cells should allow to define the active principle in plasma activated medium. Methods: Established knowledge of intercellular apoptosis-inducing reactive oxygen/nitrogen species-dependent signaling and its control by membrane-associated catalase and SOD was reviewed. Model experiments using extracellular singlet oxygen were analyzed with respect to catalase inactivation and their relevance for the antitumor action of cold atmospheric plasma. Potential interactions of this tumor cell-specific control system with components of plasma-activated medium or its reaction products were discussed within the scope of the reviewed signaling principles. Results: None of the long-lived species found in plasma-activated medium, such as nitrite and H2O2, nor OCl- or .NO seemed to have the potential to interfere with catalase-dependent control of apoptosis-inducing signaling of tumor cells when acting alone. However, the combination of H2O2 and nitrite might generate peroxynitrite. The protonation of peroxnitrite to peroxynitrous acid allows for the generation of hydroxyl radicals that react with H2O2, leading to the formation of hydroperoxide radicals. These allow for singlet oxygen generation and inactivation of membrane-associated catalase through an autoamplificatory mechanism, followed by intercellular apoptosis-inducing signaling. Conclusion: Nitrite and H2O2 in plasma-activated medium establish singlet oxygen-dependent interference selectively with the control system of tumor cells.

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