scholarly journals Biological Characterization of Locally Circulating Mycoplasma gallisepticum in Poultry

2021 ◽  
Vol 41 (01) ◽  
pp. 112-116
Author(s):  
Saba Farooq
Keyword(s):  
Field Isolate ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Mycoplasma Gallisepticum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Specific Pcr ◽  
Respiratory Discomfort ◽  
Clinical Signs And Symptoms ◽  
Experimental Group

Mycoplasma gallisepticum (MG) is a pathogen of concern for poultry. Present study was conducted to determine the biological characteristics of a field isolate of MG, recovered from an MG-affected flock. This isolation was made through conventional method of MG cultivation, using modified Frey’s media after confirming the isolate by polymerase chain reaction (PCR). A total of 48 birds were segregated into experimental group (32 birds) and the control group (16 birds). To appraise primary site of infection, MG broth propagated culture containing 1x106 CFU/ml was inoculated intratracheally to each bird in the experimental group, whereas the control group was sham inoculated by uninoculated broth. The clinical signs and symptoms were recorded daily from day 1 to 21 post-infection (p.i.). Seroconversion monitoring was carried out, at day 5, 10, 15, 20 p.i. by Serum Plate Agglutination test (SPA) and Enzyme Linked Immunosorbent Assay (ELISA). To determine the dissemination pattern of MG, birds were sacrificed according to plan, swabbed from various organs and subjected to MG-specific PCR. Tracheal lesions and air sac lesions were scored after necropsy. Clinically, mild signs of respiratory discomfort were observed on day 5 p.i., which intensified on day 9 to 21 p.i. in the experimental group. PCR of tracheal swab samples was positive from day 7 to 21 p.i., and the swabs collected from lungs were positive for MG from day 9 to 21 p.i. The study concluded that, MG isolate from field showed limited dissemination pattern and is restricted to respiratory tract.

Determination of thallium in urine, blood, and hair in illicit opioid users in Iran

2020 ◽  
Vol 39 (6) ◽  
pp. 808-815
Author(s):  
N Molavi ◽  
A Ghaderi ◽  
HR Banafshe
Keyword(s):  
Graphite Furnace ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Absorption Spectrometry ◽  
Control Group ◽  
Opioid Use ◽  
Clinical Signs And Symptoms ◽  
Opioid Users ◽  
Thallium Exposure ◽  
Illicit Opioid Use

Context: Heavy metals, including thallium and lead, are introduced to illicit drug users’ body as a result of using drugs such as cocaine and heroin. Objective: This study aimed to determine urine, blood, and hair thallium (Tl) concentrations in illicit opioid users along with the relevant clinical signs and symptoms consistent with thallotoxicosis and to compare them with the corresponding variables in the control non-opioid user group. Materials and Methods: This case–control study was conducted on 50 illicit opioid users who had abused opioids continuously for more than a year, referred to Amirie Drug Abuse Treatment Clinic in Kashan, Iran. The control group included 50 non-opioid users. Thallium concentrations in urine, blood, and hair were assessed in both groups ( n = 100) using electrothermal (graphite furnace) atomic absorption spectrometry (ET AAS, GF AAS). Results: In the studied group, the median (interquartile range) concentrations of thallium in urine, blood, and hair were 54.8 ± 79.9 μg/L, 14.5 ± 11.1 μg/L, and 5.4 ± 3.7 µg/g, respectively; these values were 4.8 ± 5.2 μg/L, 2.5 ± 2.4 μg/L, and 1.4 ± 1.1 µg/g, respectively, in the control group. There were significant differences in urine, blood, and hair thallium concentrations between the study group and the control group ( p < 0.001). There were significant correlations between duration of illicit opioid use and urine thallium concentrations ( r = 0.394, p = 0.005) and hair thallium concentrations ( r = 0.293, p = 0.039), but not with blood thallium concentrations ( r = 0.246, p = 0.085). Urine and blood thallium concentrations of illicit opioid users with clinical signs and symptoms consistent with thallotoxicosis of weakness ( p = 0.01), depression ( p = 0.03), and headache ( p = 0.03) were higher than users without these problems. Discussion and conclusion: The results of the study showed that thallium concentrations in urine, blood, and hair in illicit opioid users were significantly higher than the comparable concentrations in the control group. This can be due to the use of illicit opioids adulterated with thallium. Also, this study showed long-term illicit opioid use may lead to thallium exposure. In addition, cigarette smoking was associated with increased thallium exposure.

ELECTRODIAGNOSTIC CRITERIA FOR DIAGNOSING CARPAL TUNNEL SYNDROME NEED TO BE REVISED IN HYPOTHYROID PATIENTS

2017 ◽  
Vol 20 (03) ◽  
pp. 1750014
Author(s):  
Mahdieh Asadi ◽  
Sharareh Roshanzamir
Keyword(s):  
Carpal Tunnel Syndrome ◽  
Carpal Tunnel ◽  
Clinical Symptoms ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Control Group ◽  
Tunnel Syndrome ◽  
Clinical Signs And Symptoms ◽  
Relationship Of ◽  
Hypothyroid Patients

Background: Previous studies do not agree with each other on the association between electrodiagnostic findings and clinical symptoms of Carpal tunnel syndrome (CTS). In most of these studies, many variables such as age, sex, obesity and hypothyroidism have not been taken into account. Material & methods: About 62 patients with hypothyroidism and 62 patients without hypothyroidism with sign and symptoms of CTS were included in this study. Electrodiagnostic tests were done for all patients. And relationship of the severity of CTS signs and symptoms with electrodiagnostic parameters was examined statistically in each group. Results: This study showed that distal motor latency in control group (without hypothyroidism) is significantly more prolonged than hypothyroid patients. Also there was significant correlation between clinical symptoms and electrodiagnostic findings in control group, but there was not such correlation in hypothyroid patients. In 62% of hypothyroid patients with clinical signs and symptoms of CTS, electrodiagnostic findings were normal and only in 38% of cases, electrodiagnostic findings were suggestive of CTS. Conclusion: Relationship of the severity of CTS signs and symptoms with electrodiagnostic parameters is very weak in hypothyroid patients. Many hypothyroid patients with clinical signs and symptoms of CTS have normal electrodiagnostic findings; so we need more studies for revising the para-clinic criteria of labeling patients having CTS in hypothyroid patients.

scholarly journals Possible Association between Diabetes and Bisphosphonate-Related Jaw Osteonecrosis

2007 ◽  
Vol 92 (3) ◽  
pp. 1172-1175 ◽  
Author(s):  
Mogher Khamaisi ◽  
Eran Regev ◽  
Noam Yarom ◽  
Batia Avni ◽  
Eran Leitersdorf ◽  
...  
Keyword(s):  
Fasting Blood Glucose ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Pathological Process ◽  
Control Group ◽  
Diabetic Patients ◽  
Glucose Levels ◽  
Hospital Referral ◽  
Hebrew University ◽  
Clinical Signs And Symptoms

Abstract Context: Bisphosphonate-related osteonecrosis (BON) of the jaws is a newly identified condition for which the exact mechanism involved in its pathogenesis remains obscure. Objective: The objective of the study was to evaluate whether diabetes mellitus (DM) may be a contributing factor in the development of BON. Design: From 2004 to 2006, 31 patients were diagnosed with BON. The diagnosis of BON was based on the medical and dental history of each patient as well as the observation of clinical signs and symptoms of this pathological process. DM was based on two consecutive fasting blood glucose levels above 7 mmol/liter. Setting: The study was completed in the Hebrew University-Hadassah Hospital referral center. Results: Of the 31 patients with BON, 18 (58%) were found to have DM or impaired fasting glucose. The proportion of diabetic patients was much higher than expected relative to the incidence of DM in the general population (14%) and compared with the proportion of diabetic patients in a control group of oncological patients treated with bisphosphonates and without BON (12%) (P = 0.00003). Conclusions: This finding indicates that DM may be a risk factor for BON and that DM patients treated with bisphosphonates should be carefully monitored. We discuss here the bone metabolic pathways characteristic of DM patients and the way in which these pathways can augment the effects of bisphosphonates.

scholarly journals The comparison of the efficacy of Alveogyl, 0.8% Hyaluronic acid, and 0.2% Chlorhexidine Digluconate in alveolar osteitis

2021 ◽  
Vol 11 (1) ◽  
pp. 6-11
Author(s):  
Ömür Dereci ◽  
Görkem Tekin ◽  
Yasin Çağlar Koşar
Keyword(s):  
Hyaluronic Acid ◽  
Clinical Signs ◽  
Physiological Saline ◽  
Signs And Symptoms ◽  
Control Group ◽  
Chlorhexidine Digluconate ◽  
Saline Irrigation ◽  
Significant Difference ◽  
Alveolar Osteitis ◽  
Clinical Signs And Symptoms

Aim:  The aim of this study was to compare the efficacy of Alveogyl, 0.8% hyaluronic acid (HA), and 0.2% chlorhexidine digluconate (CHX) gel in reducing pain and improving clinical signs and symptoms of alveolar osteitis. Methodology: The clinical data of patients treated for alveolar osteitis between 01/01/2015 and 01/01/2019 were retrieved for this study. All patients were initially treated by curettage and physiological saline irrigation. Patients were then divided into 4 groups. Group 1 was considered the control group; no other biomaterials were administered after curettage and physiological saline irrigation. All other groups were administered an additional treatment in the socket after curettage and physiological saline irrigation (Group 1 – Alveogyl; Group 2 - 0.8% HA; Group 3 - 0.2% CHX). Patents were evaluated before surgery as well as days 3 and 7 after surgery. The postoperative evaluations included: Visual analog scale(VAS) pain scores, the presence of clinical signs and symptoms of exposed alveolar bone, disorganized blood clot, inflammation around the socket, and bad odor and taste. Results: Sixty-seven patients were included in the study. There was no statistically significant difference between groups in all control evaluations (p>0.05). There was significantly reduced inflammation around the extraction socket on postoperative day 7 in the CHX group compared to that in the control group (p<0.05). No other significant changes in clinical signs and symptoms were observed among groups. Conclusion: There was no significant difference between curettage with physiological saline irrigation alone and the addition of Alveogyl, 0.8% HA, or 0.2% CHX in the reduction of pain in alveolar osteitis. Nonetheless, CHX may reduce inflammation around the extraction sockets.   How to cite this article: Dereci Ö, Görkem T, Koşar YÇ. The comparison of the efficacy of Alveogyl, 0.8% Hyaluronic acid, and 0.2% Chlorhexidine Digluconate in alveolar osteitis. Int Dent Res 2021;11(1):6-11. https://doi.org/10.5577/intdentres.2021.vol11.no1.2   Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

scholarly journals Magnitude of Rotavirus A and Campylobacter jejuni infections in children with diarrhea in Twin cities of Rawalpindi and Islamabad, Pakistan

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Asma Sadiq ◽  
Habib Bokhari ◽  
Zobia Noreen ◽  
Rai Muhammad Asghar ◽  
Nazish Bostan
Keyword(s):  
Campylobacter Jejuni ◽  
Acute Diarrhea ◽  
Detection Rate ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Public Health Care ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rotavirus A ◽  
Specific Pcr ◽  
Species Specific

Abstract Background Acute diarrhea is a leading cause of morbidity and mortality in children particularly in developing countries of Asia and Africa. The present study was conducted to detect the two most important pathogens, rotavirus and Campylobacter Jejuni in children suffering with diarrhea in Rawalpindi and Islamabad, Pakistan in 2014. The clinical and epidemiological aspects of the disease were also investigated. Methods A total of 500 stool samples were collected from children presented with clinical signs and symptoms of acute diarrhea. The samples were initially screened for the presence of rotavirus A (RVA) via ELISA (Enzyme-linked immunosorbent assay) and RT-PCR (Reverse Transcriptase PCR) and then were analysed for C. jejuni by using species specific PCR assay. Results The detection rate of RVA was 26.4% (132/500) while, Campylobacter was detected in 52% (260/500) of samples with C. jejuni accounted for 48.2% (241/500) of all study cases. Co-infection of C. jejuni with RVA was identified in 21.8% of all cases. Children with RVA and C. jejuni co-infection showed a higher probability (p = 0.01) to be dehydrated. A significant association (p = 0.02) was found between C. jejuni positive status and fever in children. The median age of children with both RVA and C. jejuni infection was 6–11 months. The RVA detection rate was high in winter months of the year while, C. jejuni infections were documented high in summer over 1 year study period. Conclusions The overall results have demonstrated the high prevalence of C. jejuni in Rawalpindi, Islamabad, Pakistan in 2014. The results of present study will not only help to calculate disease burden caused by C. jejuni and rotavirus but also will provide critical information to health authorities in planning public health care strategies against these pathogens.

Clinical value of (1,3)-β-D-glucan, mannan, antimannan IgG and IgM antibodies in diagnosis of invasive candidiasis

2019 ◽  
Vol 57 (8) ◽  
pp. 976-986
Author(s):  
Fengtian Li ◽  
Xiaotian Yu ◽  
Liyan Ye ◽  
Guang Zhou ◽  
Leili Wang ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Invasive Candidiasis ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Immunoglobulin M ◽  
Control Group ◽  
Clinical Value ◽  
Serum Samples ◽  
Clinical Signs And Symptoms ◽  
And Control

Abstract Diagnosis of invasive candidiasis (IC) is still challenging due to absence of specific clinical signs and symptoms. In this study we investigate the clinical value of (1,3)-β-D-glucan (BDG), mannan (MN), antimannan immunoglobulin G (AM-IgG), and antimannan immunoglobulin M (AM-IgM) assay in diagnosis of IC. During 2016 to 2018 serum samples from 71 patients with IC and 185 patients without IC were collected. Serum samples from 41 patients with bacteremia were also enrolled as additional control. Significant differences in mean serum biomarkers levels between IC and control group were observed. At low cutoff threshold the sensitivity and specificity of BDG (70 pg/ml), MN (50 pg/ml), AM-IgG (80 AU/ml), and AM-IgM (80 AU/ml) assay were 64.8% and 90.8%, 64.8 and 89.2%,74.6% and 87.0%, 57.7% and 60.0%, respectively. Combined use of BDG/MN, BDG/AM-IgG and MN/AM-IgG improved the sensitivity and specificity to 85.9% and 81.1%, 85.9% and 80.0%, 81.7% and 81.6%, respectively. The combination of BDG/MN, BDG/AM-IgG, or MN/AM-IgG may provide an encouraging approach for diagnosis of IC.

scholarly journals Open Comparison Study between Augmentin and Ampicillin-Chloramphenicol in the Treatment of Bronchopneumonia in Children

2018 ◽  
Vol 35 (9-10) ◽  
pp. 222-6
Author(s):  
M. Hardjono Abdoerrachman
Keyword(s):  
Clinical Signs ◽  
Signs And Symptoms ◽  
Clinical Effectiveness ◽  
Control Group ◽  
Study Group ◽  
Comparison Study ◽  
Good Clinical Response ◽  
Single Drug ◽  
Clinical Signs And Symptoms ◽  
The Given

Sixty children under 2 years of age suffering from bronchopneumonia were evaluated for the effectiveness of the treatment. The first group consisted of 30 patients was treated with a single drug, i.e. , Augmentin (amoxycillin and clavulanic acid) as a study group. The second group was treated with a combination of ampicillin and chloramphenicol as a control group. The two groups showed good clinical response to the the given treatment. Clinical signs and symptoms of bronchopneumonia disappeared within the similar period in both groups, including decrease of fever, disappearance of dyspnea, and diminution or disappearance of rales. The clinical effectiveness in this study, as measured by the disappearance of clinical signs and symptoms, was considered to have a success rate of 82.1% in the study group and 72.2% in control group.

Application of transcutaneus electroneurostimulation in complex therapy and rehabilitation of diabetic neuropathic pain

10.12737/7217 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 0-0
Author(s):  
Аль-Замиль ◽  
M. Al-Zamil ◽  
Божко ◽  
S. Bozhko ◽  
Кудаева ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Lower Extremity ◽  
High Frequency ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Control Group ◽  
Control Groups ◽  
Clinical Signs And Symptoms ◽  
And Control ◽  
Low Amplitude

. In this article expediency of application of monophasic, high frequency and low amplitude transcutaneous neuroelectrostimulation in treatment of neuropathic pain in patients with diabetic distal polyneuropathy of lower extremity was proved. This study included 159 patients with acute distal neuropathic pain in lower extremity. In all patients di-abetic mellitus 2 type was diagnosed and clinical signs and symptoms of distal polyneuropathy were found. Comparative dynamics of clinical and electromyographic manifestations of diabetic distal polyneuropathy between basis and control groups were analyzed. 62 patients in control group undergone treatment by the Duloxetine 60 mg every day within 3 months. In basis group 97 patients in addition to the Duloxetine were treated by transcutaneous neuroelectrostimulation during 30 minutes per day after day for 15 days. This study showed that complex treatment by combination use of transcutaneous neuroelectrostimula-tion with central analgesic more effective than traditional analgesic therapy in treatment of neuropathic pain in patients with diabetic distal polyneuropathy.

The Effects of Bonny Light Crude Oil (Blco) on the Helminth Parasite Heligmosomoides Bakeri (Polygrus) of Mice.

2019 ◽  
Vol 2 (1) ◽  
pp. 88-99
Author(s):  
V. E. Chukwu
Keyword(s):  
Crude Oil ◽  
Larval Mortality ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Treated Group ◽  
Control Group ◽  
Standard Procedures ◽  
Heligmosomoides Bakeri ◽  
Albino Mice ◽  
Clinical Signs And Symptoms

This study examined the effect of crude oil on Heligmosomoides bakeri in albino mice. A total of 35 albino mice of 5-8 weeks were used for this study. The mice were randomly divided into five groups (A, B, C, D and E) of 5 mice each and infected with 0.13ml of H. bakeri larvae. Mice in the first two groups (A and B) were given crude oil per os at the concentrations of 0.2 and 0.05 mg/ml respectively. Those in group C were given Albendazole and group D infected but not treated. Group E was used as a naïve control group. Two other groups F and G were used for toxicity test. All mice were observed for clinical signs and symptoms of abnormality all through the study and all parameters were assessed following standard procedures for 5 weeks. Administration of crude oil per os to mice in groups A and B at the dose levels used in this study showed no significant (p>0.05) changes in PCV, body weight and fecal egg counts of the mice. There was a significant (p<0.05) difference in larval mortality test between the crude oil treated groups compared to the control group by their movement (if normal, moving or dead i.e. no observed motion within 10 mins). The Albendazole group C had the least worm burden and fecal egg count compared to the groups treated with different concentration of Crude oil. In conclusion, it may be suggested that heavily infected animals may not respond to treatment with crude oil against nematodes which discredit the unorthodox folklore medicine for parasitic treatment by some rural. Its effect on the worms appeared to be transient.

scholarly journals New Approach for Diagnosis of Candidemia Based on Detection of a 65-Kilodalton Antigen

2009 ◽  
Vol 16 (11) ◽  
pp. 1538-1545 ◽  
Author(s):  
Rodrigo Berzaghi ◽  
Arnaldo Lopes Colombo ◽  
Antonia Maria de Oliveira Machado ◽  
Zoilo Pires de Camargo
Keyword(s):  
Tertiary Care ◽  
Clinical Signs ◽  
Bloodstream Infections ◽  
Signs And Symptoms ◽  
Enzyme Linked Immunosorbent Assay ◽  
New Approach ◽  
Clinical Signs And Symptoms ◽  
Initial Clearance ◽  
Delays In Diagnosis ◽  
Tertiary Care Hospitals

ABSTRACT Nosocomial candidiasis is a major concern in tertiary care hospitals worldwide. This infection generally occurs in patients with degenerative and neoplastic diseases and is considered the fourth most frequent cause of bloodstream infections. Diagnosis of candidemia or hematogenous candidiasis has been problematic because clinical signs and symptoms are nonspecific, leading to delays in diagnosis and, consequently, delays in appropriate antifungal therapy. We developed an inhibition enzyme-linked immunosorbent assay (ELISA) for detection of a 65-kDa antigen in an experimental model of candidemia and for diagnosis of patients in intensive care units (ICUs) with suspected candidemia. An anti-65-kDa monoclonal antibody was tested for detection of the 65-kDa antigen produced by Candida albicans, Candida tropicalis, and Candida parapsilosis in murine candidemia models. The 65-kDa antigen was detected in sera at concentrations ranging from 0.012 to 3.25 μg/ml. A total of 20 human patients with candidemia were then evaluated with the inhibition ELISA using sequential sera. Sixteen (80%) patients had the 65-kDa antigen in concentrations ranging from 0.07 to 5.0 μg/ml. Sequential sera from patients with candidemia presented three different patterns of antigenemia of the 65-kDa molecule: (i) total clearance of antigenemia, (ii) initial clearance and relapse of antigenemia, and (iii) partial clearance of antigenemia. Our results indicate detection of the 65-kDa protein may be a valuable tool for the diagnosis of candidemia by C. albicans, C. tropicalis, and C. parapsilosis.

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