scholarly journals Chemical Constituents of Buah Makasar [Brucea Javanica (L) Merr] Leaves and Seed Extract

2019 ◽  
Vol 2 (2) ◽  
pp. 99
Author(s):  
Handa Muliasari ◽  
Candra D Hamdin ◽  
Agus Dwi Ananto ◽  
Muhsinul Ihsan
Keyword(s):  
Methanol Extract ◽  
Chemical Constituents ◽  
Chemical Compounds ◽  
Seed Extract ◽  
Chemical Compositions ◽  
Phytochemical Screening ◽  
Brucea Javanica ◽  
Ms Analysis ◽  
Local Plants ◽  
Lombok Island

Buah Makasar [Brucea javanica (L) Merr] is one of the local plants in Lombok Island that is traditionally used for diabetic medication. The purpose of this research was to identify the chemical constituents containing in methanol leaves and seed extract of Buah Makasar. The methods used were phytochemical screening for some groups of chemical compounds and GC-MS analysis. The result of phytochemical screening showed that methanol extract of B. javanica seed contains alkaloids, terpenoids, and tannin with a high intensity; while the leaves extract contains alkaloids, flavonoids, steroids, tannin, and saponin. The differences of chemical compositions in the leaves and seed extract may give them have different bioactivities. GC-MS analysis showed some fatty acids containing in the seed extract such as stearic acid and palmitic acid.

scholarly journals Isolasi Senyawa Kimia Stigmastan-3,5-Diena Yang Mempunyai Daya Toksik Dari Daun Ekaliptus (Eucalyptus Deglupta Blume.)

2017 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Sari Setianingsih ◽  
Rudi Kartika ◽  
Partomuan Simanjuntak
Keyword(s):  
Ethyl Acetate ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Test Results ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Ms Analysis

This study was started by extraction of Eucalyptus deglupta Blume. Using organic solvent   (n-hexane, ethyl acetate, ethanol and water) followed by phytochemical screening and toxicity test using Brine Shrimp Lethality Test (BSLT) method. Isolation and identification of chemical compounds contained in the fraction were done by column chromatography and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Phytochemical screening revealed the presence of alkaloids, flavonoids, steroids and phenolics in the extract. Toxicity test results showed that the ethyl acetate extract was potentially active with LC50 value of  617.95 ppm. The extract was continued to isolation stage and gave fraction EKEA-3.1 with LC50 value of 2759.93 ppm. Identification of chemical compounds in EKEA-3.1 with KG-MS analysis showed that EKEA-3.1 was suspected to be Stigmastan-3,5-diene.

scholarly journals Phytochemical Screening And Tyrosinase Inhibition Activity Of Leaves Cassia siamea L.

2020 ◽  
Vol 12 (2) ◽  
pp. 198
Author(s):  
Munawarohthus Sholikha ◽  
Ainun Wulandari
Keyword(s):  
Methanol Extract ◽  
Chemical Constituents ◽  
Tropical Region ◽  
Chloroform Fraction ◽  
Phytochemical Screening ◽  
Tyrosinase Inhibition ◽  
Cassia Siamea ◽  
Percent Inhibition ◽  
Butanol Fraction ◽  
Chemical Content

Cassia siamea L. has been used traditionally as medicine and in previous studies had an IC­50 antioxidant activity of 144.12 µg / mL. Cassia siamea L. potential as a medicinal substance is thought to have chemical constituents such as saponins, anthraquinones, alkaloids, flavonoids, tannins, terpenoids and steroids. Indonesia, which is located in a tropical region with high temperatures and ultraviolet (UV) radiation, can cause skin disorders such as hyperpigmentation due to excess melanin synthesis. Tyrosinase enzymes can prevent or inhibit melanin formation. The purpose of this study was to determine the chemical content of Cassia siamea L. leaves extract with various solvents and the inhibitory activity of tyrosinase enzymes. In this study, the leaves of Cassia siamea L. were macerated with methanol as a solvent. The dry methanol extract was fractionated using the liquid-liquid method using aquadest, butanol and chloroform as solvents. The four dried extracts were then phytochemical screening to determine their chemical content. After that, the tyrosinase inhibition test was carried out in vitro with L-Dopa as a substrate using ELISA plate well reader at concentrations of 100, 1000 and 100000 ppm with three repetitions (triplo). The methanol extract had percent inhibition of 19.993 ± 1.125%, 28.984 ± 0.624%, 57.164 ± 0.623%, respectively; butanol fraction 25.914 ± 0.541%, 32.566 ± 0.767%, 52.120 ± 1.616%; chloroform fraction 19.920 ± 0.730%, 26.425 ± 0.937%, 62.865 ± 0.167% and aquadest fraction 23.830 ± 0.879%, 30.885 ± 0.778%, 67.471 ± 0.352%. Kojic acid had tyrosinase activity and acts more active than the other extract and fraction with 91.155±0.228% at 500 ppm. The butanol fraction had the highest percent inhibition at 100 and 1000 ppm which was thought to be due to the presence of alkaloids, tannins, flavonoids, steroids and terpenoids from the results of the phytochemical screening test.

scholarly journals Hair Washing Formulations from Aloe elegans Todaro Gel: The Potential for Making Hair Shampoo

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Desta Berhe Sbhatu ◽  
Goitom Gebreyohannes Berhe ◽  
Abadi Gebreyesus Hndeya ◽  
Asmael Abdu ◽  
Afework Mulugeta ◽  
...  
Keyword(s):  
Foam Stability ◽  
Chemical Constituents ◽  
Coconut Oil ◽  
Capric Acid ◽  
Lemon Juice ◽  
Solid Content ◽  
Phytochemical Screening ◽  
Ms Analysis ◽  
Physicochemical Methods ◽  
Gas Chromatography Mass Spectroscopy

This study aimed to describe the gross phytochemical constituents of Aloe elegans Todaro gel and evaluate the characteristics and quality of lab-made hair washing formulations prepared from the gel to show its potential in formulating hair washing shampoos. A. elegans gel mass was prepared from mature, healthy leaves collected from natural stand. Samples of 100% methanol extract of the gel were subjected to standard phytochemical screening and gas chromatography-mass spectroscopy (GC-MS) analysis. Five hair washing formulations (Fs) were, likewise, prepared by mixing 4.0–10.0 mL of gel with one (0.05 mL) to two (0.10 mL) drops of six synthetic and natural ingredients, namely, coconut oil, jojoba oil, olive oil, pure glycerin oil, lemon juice, and vitamin E. The gel to the total ingredient ratios (v/v) of the five formulations were 93 : 7 (F1), 94.5 : 5.5 (F2), 96.4 : 3.6 (F3), and 96.6 : 3.4 (F4 and F5). The formulations were evaluated using sensory inspection and common physicochemical methods. The phytochemical screening and GC-MS analysis revealed that A. elegans gel is the source of important chemical constituents used in the formulation of shampoos and similar products including saponins, capric acid, lauric acid, myristic acid, palmitic acid, linoleic acid, stearic acid, and phytol. Lab-made A. elegans hair washing formulations, especially those with 96.4–96.6% gel, were found to have similar characteristics and qualities with a common marketed shampoo. All the formulations were turbid with characteristic odor as the marketed shampoo. The pH values of the hair washing formulations (6.4–4.6) were comparable to those of the marketed shampoo (6.7). Formulations with higher proportion of gel had better foam stability, higher solid content (26–29%), higher surface tension (33–38 dynes/cm), shorter wetting time (150–160 sec), equivalent viscosities (26.45–26.73 poise), and conditioning performance than the marketed shampoo. These findings demonstrate that A. elegans gel mass can be used in the formulation of good-quality hair washing shampoos. We recommend future studies that aim to develop the phytochemical profile of the plant and a refined protocol of hair washing shampoo formulation.

scholarly journals Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)

2016 ◽  
Vol 66 (3) ◽  
pp. 387-398 ◽  
Author(s):  
Ezzat Abdel-Lateef ◽  
Faten Mahmoud ◽  
Olfat Hammam ◽  
Eman El-Ahwany ◽  
Eman El-Wakil ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Line ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Curcuma Longa ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Ms Analysis ◽  
Phenolic Constituents

Abstract The present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a hepatoma cell line. The methanolic extract was subjected to GC-MS analysis to identify the volatile constituents and the other part of the same extract was subjected to liquid column chromatographic separation to isolate curcumin. The inhibition of cell growth in the hepatoma cell line and the cytopathological changes were studied. GC-MS analysis showed the presence of fifty compounds in the methanolic extract of C. longa. The major compounds were ar-turmerone (20.50 %), β-sesquiphellandrene (5.20 %) and curcumenol (5.11 %). Curcumin was identified using IR, 1H and 13C NMR. The inhibition of cell growth by curcumin (IC50 = 41.69 ± 2.87 μg mL-1) was much more effective than that of methanolic extract (IC50 = 196.12 ± 5.25 μg mL-1). Degenerative and apoptotic changes were more evident in curcumin- treated hepatoma cells than in those treated with the methanol extract. Antitumor potential of the methanolic extract may be attributed to the presence of sesquiterpenes and phenolic constituents including curcumin (0.051 %, 511.39 μg g-1 dried methanol extract) in C. longa rhizomes.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF METHANOLIC LEAF EXTRACT OF DRYPETES SEPIARIA (WIGHT & ARN.) PAX. & HOFFIM. FROM SILAMBUR SACRED GROVE, TAMILNADU

2017 ◽  
Vol 4 (2) ◽  
pp. 157-161
Author(s):  
Saravanakumar K
Keyword(s):  
Extraction Method ◽  
Methanol Extract ◽  
Soxhlet Extraction ◽  
Sacred Grove ◽  
Phytochemical Screening ◽  
Plant Materials ◽  
Ms Analysis ◽  
Successive Extraction ◽  
Phytochemical Compounds ◽  
Phytochemical Constituents

Drypetes sepiaria (Wight & Arn.) Pax. & Hoffim a medium sized tree member of Euphorbiaceae was investigated to determine the phytochemical constituents present in various extracts of the leaves through GC-MS analysis. Powdered leaf plant materials were subjected to successive extraction with organic solventssuch as methanol by Soxhlet extraction method. In the present study, GC-MS analysis revealed that a total of 23 different compounds identified by using methanol extract and all the identified compounds were medicinally valuable for the treatment of various human ailments. In addition, all the phytochemical compounds were needed for further investigations on toxicological aspects for the development of new lead of therapeutic interest.

scholarly journals Phytochemical Analysis and Antifungal Activity of Ficus Sycomorus L. (Moraceae) Extracts on some Dermatophytic Fungi

2021 ◽  
Vol 6 (1) ◽  
pp. 11-17
Author(s):  
Umar Lawal ◽  
◽  
L. Mansir ◽  
M. G. Sani
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Fungal Infections ◽  
Stem Bark ◽  
Phytochemical Analysis ◽  
Microsporum Canis ◽  
Phytochemical Screening ◽  
Ms Analysis ◽  
Dermatophytic Fungi ◽  
Ficus Sycomorus

Ficus sycomorus L is a large, semi-deciduous spreading savannah tree that belongs to the family (Moraceae) is used by many tribes in the treatments of skin infections. The aim of this study was to carry out phytochemical analysis and antifungal evaluation of F. sycomorus L. stem and leaf Methanol extract on some dermatophytic fungi. The stem and leaf crude extracts were obtained after drying using maceration and percolation with methanol as the solvent. The extract was subjected to qualitative phytochemical screening as well as GC-MS analysis for the presence of bioactive constituents. The antifungal inhibitory effect of the crude stem and leaf extracts was assessed by agar well diffusion methods. The phytochemical screening revealed that the leaves and stem-bark contained secondary metabolites such as tannins, flavonoids, alkaloids, saponins, reducing sugar and steroid. The GC-MS assay showed that the most prevailing compound in the methanolic stem-bark extract was 2, 9, Diazabicyclo (4, 4, 0) decane-3,10 dione, while in the methanolic leaves extract was acetic acid. The anti-dermatophytic assay of the extracts against Microsporum canis and Tricophyton tonsurans showed that the extracts inhibited the fungi with zones of inhibitions ranging from 6.33±0.47 to 24.33±0.47mm with the leaf extract exhibiting higher inhibition zones than the stem extract (p˂0.01). The study shows that both the stem and leaf methanol extract of Ficus sycomorus L possesses antifungal activity and should be considered as a potential antifungal agent in search of newer agents to address dermatophytic fungal infections Key words: Ficus sycomorus L, Phytochemical/GC-MS Analysis, Antifungal activity

Detection of Adulteration in Rubia cordifolia – A Chromatographic Approach

2021 ◽  
pp. 4013-4018
Author(s):  
Ghanshyam Kamani ◽  
Rohan Sanghani ◽  
Vaibhavi Savalia ◽  
Devang Pandya
Keyword(s):  
Mobile Phase ◽  
Methanol Extract ◽  
Skin Diseases ◽  
Chemical Constituents ◽  
Raw Material ◽  
Rubia Cordifolia ◽  
Methanol Extracts ◽  
Rubia Tinctorum ◽  
Ms Analysis ◽  
The One

According to traditional medicinal texts as well as modern research, Rubia cordifolia (Desi Manjistha) stem cure various diseases of blood, skin diseases, inflammations, kidney stones, fever and various Kapha disorders. However, stems of Rubia cordifolia (Desi Manjistha) are often adulterated with stems of Rubia tinctorum (Irani Manjistha) due to morphological similarity. This adulteration is extremely harmful as R. tinctorum has Lucidin as one of the chemical constituents, which has severe genotoxic effects. The present work focuses on developing a comparative HPTLC fingerprint and GC-MS analysis of R. cordifolia and R. tinctorum, so as to detect the adulteration by R. tinctorum in R. cordifolia raw material form and in formulations. Methanol extracts of the individually powdered stems were used to develop a suitable mobile phase, such that a clear spot was obtained in TLC of R. tinctorum but not in TLC of R. cordifolia using the same mobile phase. This mobile phase was further used to develop a comparative HPTLC fingerprint of the two species. Methanol extracts of R. cordifolia and R. tinctorum were used for investigation of phytoconstituents by GC-MS analysis. The mobile phase n-butanol: ethanol (6:4) was the one which gave a clear single spot at Rf 0.89 in TLC of methanol extract of R. tinctorum but not in TLC of methanol extract of R. cordifolia, at 254nm and 366nm. Further, HPTLC analysis gave results clearly differentiating the two species using the same mobile phase. Further, GC-MS analysis of R. cordioflia revealed the presence of 9 phytoconstituents while R. tinctorum revealed the presence of 6 phytoconstituents, which are different from each other. Thus, these simple yet sophisticated techniques will be very useful for herbal industry in standardization and detection of adulteration of R. tinctorum in R. cordifolia formulations, thereby benefitting the patients who consume Manjistha formulations.

scholarly journals Determination of Plant Constituents in Fractions of Cordia obliqua Willd. Leaf Methanol Extract using GC-MS Analysis

2017 ◽  
Vol 9 (09) ◽  
Author(s):  
R. Gupta ◽  
G. D. Gupta
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Deciduous Tree ◽  
Sesquiterpene Alcohol ◽  
Ms Analysis ◽  
Plant Constituents ◽  
Extract Fraction ◽  
Important Medicinal Plant

Cordia obliqua Willd. is an important medicinal plant of family Boraginaceae. It is commonly known as Clammy Cherry. It is a deciduous tree and found throughout Himalayan region in India. Traditionally, it possesses a number of medicinal activities like diuretic, expectorant, antipyretic, anthelmintic and maturant. The current work is aimed to investigate chemical constituents present in fractions of leaf methanol extract of Cordia obliqua plant using GC-MS analysis. Two fractions of methanol extract (CO1 and CO3) were studied and about 30 components were detected in each sample. Highest peak area was observed for moncyclic sesquiterpene alcohol α-Bisabolol oxide b at RT 20.84 in CO1 and for o-methyl-d-glucose at RT 16.53 in CO3. The important constituents detected were Amino acids, Oleic acid, Oleanolic acid, Sesquiterpene lactone, trietrpenoids, various steroids, galactopyranoside, Hexadecanoic acid, Phthalic acid, Azafrin, Decatrienoic acid, Docasenamide, Morphinan and carotenoids. Among these Morphinan is an opoid alkaloid and good psychoactive drug. Other constituents are also medicinally important and many have reported biological activities. Thus GC-MS analysis revealed the existence of various types of constituents in Cordia obliqua leaf methanol extract fraction which confirm the application of this plant for a number of medicinal activities.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAN SKRINING FITOKIMIA EKSTRAK HEKSANA, ETIL ASETAT DAN METANOL TUMBUHAN SURUHAN (Peperomia pellucida L. Kunth)

2020 ◽  
Vol 16 (2) ◽  
pp. 105-111
Author(s):  
Restianti Restianti ◽  
Bimo Budi Santoso ◽  
Maria Ludya Pulung
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
E Coli ◽  
Activity Test ◽  
Solvent Analysis

Antibacterial activity test and screening of phytochemical of extract hexane, ethyl acetate  and methanol have been carried out. The extraction of the P.pellucida plants was carried out by the maceration method in stages based on the polarity of the solvent. Analysis of chemical compounds done with phytochemical screening test. Based on the identification of chemical compounds by phytochemical screening, hexane and ethyl acetate extract has been detected only in the presence of flavonoid compounds with moderate categories. Methanol  extract contains high-grade alkaloids and moderate flavonoid and tannin compounds. Antibacterial test using disffusion methods suggest that ethyl acetate and methanol extract have categorically strong antibacterial activity againts the S. aureus  of 14 mm and 16 mm respectively. Hexane extract and acetate ethyl have a strong inhibition to  E. coli  of 11 mm and 12 mm respectively. Methanol extract has a weak inhibition to bacteria E. coli  and a hexane extract has a weak inhibition to bacteria S. aureus.

scholarly journals Identification of chemical compositions in some medicinal plants by GC/MS analysis

2019 ◽  
Vol 20 (1) ◽  
pp. 161-172
Author(s):  
S. Mohammadi ◽  
◽  
Kh. Piri ◽  
M. Dinarvand ◽  
◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Methanol Extract ◽  
Chemical Compounds ◽  
New Drugs ◽  
Gas Chromatography Mass Spectrometry ◽  
Vitamin B ◽  
Chemical Compositions ◽  
Potential Sources

Medicinal plantsused in treatment of diseases earlier times, are potential sources of new drugs. In this study, chemical compositions of Echium khuzestanicum, Marrubium anisodan and Echinops cephalotes were identified by Gas Chromatography-Mass Spectrometry(GC/MS).M.anisodanand E.cephalotes aerial organs and E.khuzistanicum flowers were extracted by maceration method. According to the results, 46 kinds of chemical compounds found in methanol extract of E.khuzestanicum. There are mucilage, fatty acids, flavonoid and diterpenes in the flower of E.khuzestanicum. Seventy sixkinds of chemical compounds were found in methanol extract of E.cephalotesincludingaldehydes (7.9%), phenols (7.5%), fatty acids (5.8%) and furfural (5.4%)and86 kinds of chemical compounds found in M.anisodan extract. Furfural, steroids, vitamin B and flavonoids are the main compounds of M.anisodan.

Sign in / Sign up

Export Citation Format

Share Document