scholarly journals ANALISIS SENSORI DAN AKTIVITAS ANTIOKSIDAN MENGGUNAKAN METODE DPPH PADA CAMPURAN BAWANG PUTIH, JAHE, LEMON DAN MADU SEBAGAI SUPLEMEN HERBAL

Pro Food ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 600
Author(s):  
Suci Rahmi ◽  
Hasanuddin Husin
Keyword(s):  
Antioxidant Activity ◽  
Sensory Analysis ◽  
The Body ◽  
Herbal Supplement ◽  
Herbal Supplements ◽  
Treatment Techniques ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Herbal Plant

ABSTRACT Herbal supplements are one of the products that are taken from outside the body derived from a mixture of herbal plant ingredients have antioxidant activity to prevent the presence of free radicals in the body. Testing natural antioxidant activity in herbal supplement products using the DPPH method (1,1-diphenyl-2-picrylhyrazil). Measurement of UV-Visible spectrophotometry absorbance with DPPH absorbance value at a wavelength of 517 nm. Sensory analysis using a hedonic test at the panelist preference level, using four attribute parameters namely taste, aroma, color and overall acceptance. Results Tests of antioxidant activity in herbal supplement products on various combination techniques and concentrations of garlic, ginger, local lemons and honey, resulting in the reduction of DPPH which is characterized by a reduction in the intensity of the color from purple to fade to yellow. Antioxidant testing obtained IC50 values ​​from all herbal supplement samples from various treatment techniques and concentrations showed IC50 values ​​less than 50 found in the treatment of chopped engineering materials, concentration 1 (K1) of 23.97%. While concentrations of 2 (k2) and 3 (k3) as well as in various combination techniques and other concentrations indicate that IC50 values ​​range from 50 ppm to 100 ppm. This shows that herbal supplements have very strong antioxidants (IC50 value <50) found in the treatment of ingredients with chopped techniques with the lowest concentration. whereas herbal supplements with treatment techniques and other concentrations have a strong antioxidant value of IC50 (50-100). The results of sensory analysis indicate that the average panelist had a preference level for herbal supplement products with chopping and extraction treatment techniques. Keywords: Antioxidant, DPPH, Herbal supplements, IC50   ABSTRAK   Suplemen herbal merupakan salah satu produk yang menjadi asupan dari luar tubuh yang berasal dari hasil campuran bahan tanaman herbal yang mempunyai aktivitas antioksidan tinggi untuk mencegah adanya radikal bebas dalam tubuh. Pengujian aktivitas antioksidan alami pada produk suplemen herbal menggunakan metode DPPH (1,1-diphenyl-2-picrylhyrazil). Pengukuran absorbansi secara spektrofotometri-UV Visibel dengan Nilai absorbansi DPPH pada panjang gelombang 517 nm. Analisis sensori dengan menggunakan uji hedonik pada tingkat kesukaan panelis, menggunakan empat parameter atribut yaitu rasa, aroma, warna dan penerimaan keseluruhan. Hasil Pengujian aktivitas antioksidan dalam produk suplemen herbal pada berbagai teknik kombinasi dan konsentrasi bawang putih, jahe, lemon lokal dan madu, menghasilkan peredaman DPPH yang ditandai dengan berkurangnya intensitas warna ungu menjadi pudar sampai kekuningan. Pengujian Antioksidan didapatkan Nilai IC50 dari seluruh sampel suplemen herbal dari berbagai teknik perlakuan dan konsentrasi menunjukkan nilai IC50 kurang dari 50 terdapat pada perlakuan bahan teknik rajangan, konsentrasi 1 (K1) sebesar 23,97 %. Sementara konsentrasi 2 (k2) dan 3 (k3) serta pada berbagai teknik kombinasi dan konsentrasi lainnya menunjukan bahwa nilai IC50  berkisar 50 ppm - 100 ppm. Hal ini menunjukkan bahwa suplemen herbal memiliki antioksidan yang sangat kuat (nilai IC50 <50) terdapat pada perlakuan bahan dengan teknik rajangan dengan konsentrasi yang paling rendah, sedangkan suplemen herbal dengan teknik perlakuan dan konsentrasi lain memiliki nilai antioksidan kuat IC50 (50-100) ppm. Hasil analisis sensori menunjukan bahwa rata-rata panelis memiliki tingkat kesukaan pada produk suplemen herbal dengan teknik perlakuan rajangan dan perlakuan sari. Kata kunci: Antioksidan, DPPH, IC50, Sensori, Suplemen Herbal.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals Extraction, Fractionation, And Antioxidant Examination Of Polyfloral Honey Originated From Bone Prefecture South Sulawesi Province

2019 ◽  
Vol 7 (1) ◽  
pp. 86-91
Author(s):  
Fredryk W Mandey ◽  
Endah Handayani ◽  
Wahyuni Eka Nanda ◽  
Alfian Noor
Keyword(s):  
Antioxidant Activity ◽  
Water Extract ◽  
Hexane Extract ◽  
Steroid Alkaloid ◽  
South Sulawesi ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Positive Results

The aim of this research to isolate and identify and to test the antioxidant activity of extract and fraction of polyfloral honey originated from Bone regency, South Sulawesi. The results showed that methanol gave results in flavonoid, tannin, saponnin, steroid, and alkaloid with IC50 value of DPPH method of 683,153 µg/mL. DCM extract gave positive results for tannin, steroid and alkaloid with IC50 values of 701,743 µg/mL. n-hexane extract positively contains tannin and alkaloid with IC50 1709,536 µg/Ml, water extract positevely contains tannin, sapponin steroid, alkaloid with IC50 value 1698,345 µg/mL. Pure honey contains all aspects tested with IC50 values 2826,471 µg/mL. This showed extracts and sample have weak antioxidant activity.

scholarly journals Extraction, Fractionation, And Antioxidant Examination Of Polyfloral Honey Originated From Bone Prefecture South Sulawesi Province

2019 ◽  
Vol 7 (1) ◽  
pp. 86-91
Author(s):  
Fredryk W Mandey ◽  
Endah Handayani ◽  
Wahyuni Eka Nanda ◽  
Alfian Noor
Keyword(s):  
Antioxidant Activity ◽  
Water Extract ◽  
Hexane Extract ◽  
Steroid Alkaloid ◽  
South Sulawesi ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Positive Results

The aim of this research to isolate and identify and to test the antioxidant activity of extract and fraction of polyfloral honey originated from Bone regency, South Sulawesi. The results showed that methanol gave results in flavonoid, tannin, saponnin, steroid, and alkaloid with IC50 value of DPPH method of 683,153 µg/mL. DCM extract gave positive results for tannin, steroid and alkaloid with IC50 values of 701,743 µg/mL. n-hexane extract positively contains tannin and alkaloid with IC50 1709,536 µg/Ml, water extract positevely contains tannin, sapponin steroid, alkaloid with IC50 value 1698,345 µg/mL. Pure honey contains all aspects tested with IC50 values 2826,471 µg/mL. This showed extracts and sample have weak antioxidant activity.

scholarly journals Antioxidant Potential of Madura Knife Scallop (Solen sp) Extract as a Prevention of Oxidative Stress

2020 ◽  
Vol 15 (3) ◽  
pp. 382-389
Author(s):  
Mirwa Adiprahara Anggarani ◽  
Roy Januardi Irawan
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Cell Structure ◽  
Oxygen Species ◽  
Meat Extract ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Reactive Molecule

Reactive Oxygen Species (ROS) are produced by humans as a result of cellular metabolism and environmental factors such as pollutants or cigarette smoke. ROS is a very reactive molecule and has the ability to damage cell structure. Oxidative stress is a condition between oxidants and antioxidants that is not balance, pathophysiologically, oxidative stress can trigger the risk of various diseases including hypertension, atherosclerosis, diabetes, heart failure, stroke and other chronic diseases. Oxidative stress can be overcome by intake of antioxidants. The purpose of this study was to determine the antioxidant activity contained in the meat and shells of a knife scallop (Solen sp.) that lives in Bangkalan waters, Madura. The method used in this study is an analysis of antioxidant activity with the DPPH method. Knife scallop has antioxidant activity which is evident from the IC50 values obtained. IC50 values of the shell and meat extract with ethanol solvent were 489.56 ppm and 748.49 ppm. IC50 values of the shell and meat extract with ethyl acetate solvent were 916.43 ppm and 2045.93 ppm. While the IC50 value of the shell and meat extract with chloroform solvent was 119.37 ppm and 1692.80 ppm. Based on IC50 data of knife scallop shell and meat extracts on the 3 types of solvents, it can be concluded that knife scallop’s shell and meat extract with ethanol solvent has the greatest antioxidant activity compared to ethyl acetate and chloroform solvents.

scholarly journals Potensi Antioksidan Alami pada Ekstrak Kulit Buah Jamblang (Syzigium cumini (L.) Skeels) Menggunakan Metode DPPH (The Potency of Natural Antioxidant in The Rind Extract of Jamblang (Syzigium cumini (L.) Skeels) using DPPH Method)

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Ayu Nirmala Sari ◽  
Kusdianti Kusdianti ◽  
Diky Setya Diningrat
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
The Body ◽  
Natural Antioxidant ◽  
Negative Effects ◽  
Ic50 Value ◽  
Dpph Method ◽  
Antioxidant Potency

AbstrakStress oksidatif pada tubuh dapat memicu berbagai penyakit. Stress oksidatif ini disebabkan karena radikal bebas berlebih. Tubuh memerlukan antioksidan untuk mengurangi pengaruh radikal bebas dan meredam dampak negatifnya. Penelitian ini bertujuan untuk mengevaluasi daya antioksidan ekstrak kulit buah jamblang (Syzigium cumini (L.) Skeels) dengan menggunakan metode DPPH. Berdasarkan hasil pengujian daya antioksidan ekstrak kulit buah jamblang diketahui bahwa ekstrak kulit buah jamblang memiliki kandungan antioksidan yang tergolong aktivitas sedang dengan nilai IC50 sebesar 169.3. Hasil penelitian ini menunjukkan bahwa kulit buah jamblang berpotensi untuk dikembangkan sebagai sumber antioksidan alami bagi manusia.Kata Kunci : antioksidan, DPPH, ekstraksi, jamblang, radikal bebas, stress oksidatif AbstractOxidative stress can induce many diseases. Oxidative stress is caused by excessive free radicals in the body. The body required antioxidant to decrease and hush the negative effects of free radicals. The aim of this study was to evaluate the antioxidant potency of extract rind of jamblang (Syzigium cumini (L.) Skeels) using DPPH method. The result showed that the jamblang rind extract had moderate antioxidant activity and the IC50 value was 169.3.  Based on this result, jamblang rind was potential to be natural antioxidant for human.Keyword: antioxidant, DPPH, extraction, jamblang, free radicals, oxidative stress

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BAWANG HITAM (BLACK GARLIC) DENGAN VARIASI LAMA PEMANASAN

2020 ◽  
Vol 13 (1) ◽  
pp. 39-50
Author(s):  
Eva Agustina ◽  
Funsu Andiarna ◽  
Irul Hidayati
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Heating Time ◽  
The Body ◽  
Garlic Extract ◽  
Active Compounds ◽  
Rotary Evaporator ◽  
Ic50 Value ◽  
Ic50 Values ◽  
The Difference

AbstrakSenyawa radikal dalam tubuh sangat reaktif terhadap sel-sel, sehingga dapat menyebabkan berbagai penyakit. Senyawa yang mampu menangkal radikal bebas disebut antioksidan. Bawang hitam diketahui mengandung senyawa aktif fenolik dan turunannya sehingga dapat digunakan sebagai antioksidan. Penelitian tentang penggunaan bawang hitam sebagai antioksidan ini bertujuan untuk mengetahui perbedaan waktu pemanasan (15, 25, dan 35 hari) terhadap aktivitas antioksidan pada ekstrak bawang hitam. Bawang hitam diperoleh dengan cara memanaskan bawang putih selama 15, 25, dan 35 hari. Bawang hitam selanjutnya diekstraksi dengan pelarut metanol dengan metode maserasi. Filtrat hasil ekstraksi dipekatkan dengan rotary evaporator untuk mendapatkan ekstrak bawang hitam. Ekstrak bawang hitam diuji fitokimia untuk mengetahui kandungan senyawa aktif. Aktivitas antioksidan ekstrak bawang hitam ditentukan dengan metode penangkalan radikal 2,2-diphenyl-1-picrylhydrazyl (DPPH). Hasilnya menunjukkan ekstrak bawang hitam memiliki aktivitas antioksidan yang sangat kuat dengan nilai IC50 masing-masing 15 hari yaitu 2,41 µg/mL; 25 hari yaitu 2,93 µg/mL; 35 hari yaitu 2,27 µg/mL. Nilai IC50 <10 μg/mL menunjukkan bahwa ekstrak bawang hitam dengan waktu pemanasan 15, 25, dan 35 hari memiliki potensi antioksidan yang sangat kuat. Waktu pemanasan yang paling optimum dalam penangkalan radikal bebas adalah ekstrak bawang hitam dalam waktu pemanasan 35 hari, karena banyak terdapat kandungan senyawa flavonoid, tanin, sterol, dan saponin.Abstract Radical compounds in the body are very reactive to cells that can cause various diseases. Compounds that are able to ward off free radicals are called antioxidants. Black garlic are known to contain phenolic active compounds and their derivatives so that they can be used as antioxidants. Research on the use of black garlic as an antioxidant aims to determine the difference in heating time (15, 25, and 35 days) on the antioxidant activity of black garlic extract. Black garlic are obtained by heating the garlic for 15, 25, and 35 days. The black garlic is then extracted with methanol solvent by maceration method. The extracted filtrate was concentrated with a rotary evaporator to obtain the black garlic extract. Black garlic extract was tested for phytochemistry to determine the content of active compounds. The antioxidant activity of black garlic extract was determined by the radical deterrence method 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results showed that the onion extract had a very strong antioxidant activity with an IC50 value of 15 days each of 2.41 µg/mL; 25 days which is 2.93 μg/mL; 35 days which is 2.27 µg/mL. IC50 values <10 μg/mL indicate that the black garlic extract with a heating time of 15, 25, and 35 days has a very strong antioxidant potential. The most optimum heating time in deterring free radicals is black garlic extract within 35 days of heating because there are many compounds containing flavonoids, tannins, sterols, and saponins.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Etanol Batang Daun dan Bunga Jumpai (Glinus oppositifolius (L.) Aug. DC.)

2021 ◽  
Vol 6 (1) ◽  
pp. 14
Author(s):  
Astuti Amin ◽  
Andi Paluseri ◽  
Rahmat Priyandi Linggotu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Antioxidant Potential ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Stem Leaf

 Jumpai (Glinus Oppoitifolius (L.) Aug. DC) contains flavonoids compounds that can act as antioxidants by donating hydrogen so as to stabilize the lack of electrons in free radicals. This study aims to determine the antioxidant potential of stem, leaf, and flower extracts by looking at the IC50 value. The stems, leaves, and flowers were extracted by maceration using 70% l ethanol solvent. The results of the antioxidant activity test using the DPPH method (2,2-diphenyl-1-picrilhydrazil) showed very strong antioxidant activity with IC50 values of 9.523 µg / ml stem, 32.89 µg / ml leaves, and 23.07 µg / ml flowers with vitamin comparisons. C obtained IC50 value of 1,698 µg / ml. Based on these results, it can be rejected that the stems, leaves, and flowers have antioxidant activity with a very strong category against DPPH free radicals (2,2-diphenyl-1-picrilhydrazil). 

scholarly journals Antioxidant activity ethanol extract of gotu kola (Centella asiatica (L.) Urban) with DPPH method (2,2-Diphenyl-1-Pikrilhidrazil)

2020 ◽  
Vol 3 (2) ◽  
pp. 106
Author(s):  
Muhammad Ainul Yahya ◽  
Iif Hanifa Nurrosyidah
Keyword(s):  
Air Pollution ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
The Body ◽  
Antioxidant Compounds ◽  
Test Results ◽  
Ic50 Value ◽  
Dpph Method

Unhealthy lifestyles and air pollution cause the number of free radicals in the body to increase. To protect the body from free radicals, there are antioxidant compounds as an antidote and stabilize free radicals. One of the Indonesian plants that can be used as antioxidants is gotu kola (Centella asiatica (L.) Urban. Objective: This study aims to determine the antioxidant activity of the ethanol extract of gotu kola herb using the DPPH (2,2-Diphenyl-1-pikrilhidrazil) method. with IC50 value. Method: Gotu kola (Centella asiatica (L.) Urban) was extracted by the soxhletation method using 96% ethanol solvent. The testing of antioxidant activity was carried out using the DPPH (2.2 Diphenyl-1-Pikrihydrazil) method. Result: Test results of antioxidant activity The ethanol extract of gotu kola herb showed an IC50 value of 78.20 ppm. Conclusion: This indicated that the ethanol extract of gotu kola herb was included in the criteria for strong antioxidants. 

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals Antioxidant and Anti-Aging Activity of Green Mustard Ethanol Extract Gel

2021 ◽  
pp. 304-311
Author(s):  
Silvia Handayani ◽  
Edy Fachrial ◽  
Adek Amansyah ◽  
I. Nyoman Encrich Lister
Keyword(s):  
Antioxidant Activity ◽  
Oil Content ◽  
Ethanol Extract ◽  
The Body ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Ic50 Value ◽  
Dpph Method ◽  
Content Recovery ◽  
Better Than

The skin is the organ of the body that is frequently exposed to direct UV rays from the sun, which causes the formation of ROS (reactive oxygen species), leading to cell death and tissue damage. This health problem can be overcome by using antioxidants to stabilize the free radicals. Mustard as an organic substance which contains polysaccharide compounds, vitamin C, carotene, quercetin, routine compounds, kaempferol and β-cytostreol, all of these are beneficial to skin health and beauty. Therefore, this study aims to determine the antioxidant activity of the total flavonoids and phenol content of mustard ethanol extract using the DPPH method. Furthermore, the extract at concentrations of 2, 4, and 6% was also tested for its anti-aging activity using the following parameters: moisture and oil content, texture, collagen, wrinkles, pigment, sensitivity, and pores. The results showed that the antioxidant activity of the green mustard had an IC50 value of 170.7839 µg/ml, a total phenol content of 14.471 mg GAE/g extract and flavonoid content of 12.753 mg QE/g extract. The effectiveness of the aging activity of the extract was tested using formulation 6%, which is better than 2%, and 4%. Thus, the percentage of water content recovery, oil content, texture, collagen, wrinkles, stains, sensitivity and pores respectively was 36.41, 39.33, 64.07, 17.65, 65.28, 50.14, 49.10 and 35.09%.  Consequently, it was concluded that the extract has the potential of being developed into a herbal beauty product.

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