scholarly journals Antioxidant activity ethanol extract of gotu kola (Centella asiatica (L.) Urban) with DPPH method (2,2-Diphenyl-1-Pikrilhidrazil)

2020 ◽  
Vol 3 (2) ◽  
pp. 106
Author(s):  
Muhammad Ainul Yahya ◽  
Iif Hanifa Nurrosyidah
Keyword(s):  
Air Pollution ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
The Body ◽  
Antioxidant Compounds ◽  
Test Results ◽  
Ic50 Value ◽  
Dpph Method

Unhealthy lifestyles and air pollution cause the number of free radicals in the body to increase. To protect the body from free radicals, there are antioxidant compounds as an antidote and stabilize free radicals. One of the Indonesian plants that can be used as antioxidants is gotu kola (Centella asiatica (L.) Urban. Objective: This study aims to determine the antioxidant activity of the ethanol extract of gotu kola herb using the DPPH (2,2-Diphenyl-1-pikrilhidrazil) method. with IC50 value. Method: Gotu kola (Centella asiatica (L.) Urban) was extracted by the soxhletation method using 96% ethanol solvent. The testing of antioxidant activity was carried out using the DPPH (2.2 Diphenyl-1-Pikrihydrazil) method. Result: Test results of antioxidant activity The ethanol extract of gotu kola herb showed an IC50 value of 78.20 ppm. Conclusion: This indicated that the ethanol extract of gotu kola herb was included in the criteria for strong antioxidants. 

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Herba Pegagan (Centella asiatica (L.) Urban) Dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrazil)

2020 ◽  
Vol 3 (1) ◽  
pp. 46-54
Author(s):  
Iif Hanifa Nurrosyidah ◽  
Keyword(s):  
Air Pollution ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Centella Asiatica ◽  
The Body ◽  
Antioxidant Compounds ◽  
Extract Concentration ◽  
Physical Evaluation ◽  
Ic50 Value

Unhealthy lifestyles and air pollution cause the number of free radicals in the body to increase. To protect the body from free radicals, there are antioxidant compounds as an antidote and stabilize free radicals. One of the Indonesian plants that can be used as antioxidants is gotu kola (Centella asiatica (L.) Urban. This study aims to determine the antioxidant activity from hand and body lotion extract of gotu kola by DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method which is expressed as an IC50 value. Gotu Kola (Centella asiatica (L.) Urban) was extracted by the soxhletation method using a solvent etanol 96% and formulated in the from of hand and body lotion using variations of gotu kola extract concentrations of 1%, 3% and 5%. Then do a physical evaluation of three preparations of hand and body lotion to determine the best formula to be tested for antioxidant activity. Hand and body lotion preparation formula with as much gotu kola extract concentration 5% shows the best formula. The results of testing antioxidant activity on hand and body lotion showed IC50 value of 449.14 ppm. This indicates that the Antioxidant activity of gotu kola extract hand and body lotion is included in the very weak antioxidant criteria.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

2021 ◽  
Vol 18 (1) ◽  
pp. 60-67
Author(s):  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Sadam Husein ◽  
Titi Pudji Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidant ◽  
Activity Test ◽  
Impaired Liver ◽  
Ic50 Value ◽  
Dpph Method

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

scholarly journals Potensi Antioksidan Alami pada Ekstrak Kulit Buah Jamblang (Syzigium cumini (L.) Skeels) Menggunakan Metode DPPH (The Potency of Natural Antioxidant in The Rind Extract of Jamblang (Syzigium cumini (L.) Skeels) using DPPH Method)

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Ayu Nirmala Sari ◽  
Kusdianti Kusdianti ◽  
Diky Setya Diningrat
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
The Body ◽  
Natural Antioxidant ◽  
Negative Effects ◽  
Ic50 Value ◽  
Dpph Method ◽  
Antioxidant Potency

AbstrakStress oksidatif pada tubuh dapat memicu berbagai penyakit. Stress oksidatif ini disebabkan karena radikal bebas berlebih. Tubuh memerlukan antioksidan untuk mengurangi pengaruh radikal bebas dan meredam dampak negatifnya. Penelitian ini bertujuan untuk mengevaluasi daya antioksidan ekstrak kulit buah jamblang (Syzigium cumini (L.) Skeels) dengan menggunakan metode DPPH. Berdasarkan hasil pengujian daya antioksidan ekstrak kulit buah jamblang diketahui bahwa ekstrak kulit buah jamblang memiliki kandungan antioksidan yang tergolong aktivitas sedang dengan nilai IC50 sebesar 169.3. Hasil penelitian ini menunjukkan bahwa kulit buah jamblang berpotensi untuk dikembangkan sebagai sumber antioksidan alami bagi manusia.Kata Kunci : antioksidan, DPPH, ekstraksi, jamblang, radikal bebas, stress oksidatif AbstractOxidative stress can induce many diseases. Oxidative stress is caused by excessive free radicals in the body. The body required antioxidant to decrease and hush the negative effects of free radicals. The aim of this study was to evaluate the antioxidant potency of extract rind of jamblang (Syzigium cumini (L.) Skeels) using DPPH method. The result showed that the jamblang rind extract had moderate antioxidant activity and the IC50 value was 169.3.  Based on this result, jamblang rind was potential to be natural antioxidant for human.Keyword: antioxidant, DPPH, extraction, jamblang, free radicals, oxidative stress

scholarly journals Antioxidant activity from ethanol extract of red seaweed (Galaxaura rugosa)

2021 ◽  
Vol 11 (2) ◽  
pp. 79
Author(s):  
Bhayu Gita Bhernama ◽  
Witri Maulidy Ayu ◽  
Cut Nuzlia
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Red Seaweed ◽  
Oxidation Reactions ◽  
Ic50 Value ◽  
Dpph Method

Antioxidants are compounds that can delay, reduce, slow down or inhibit oxidation reactions from free radical reactions. Antioxidants donate electrons to unstable free radicals so that these free radicals can be neutralized to not interfere with the body's metabolic processes. Red seaweed Galaxaura rugosa has potential as an antioxidant. The study aimed to determine the antioxidant activity of the ethanol extract of red seaweed Galaxaura rugosa against DPPH free radicals based on the IC50 value. Phytochemical testing and determination of antioxidant activity were carried out using the DPPH method (2,2-diphenyl-1-picrylhydrazyl), which was carried out quantitatively using a UV-Vis spectrophotometer. The results of the phytochemical screening of red seaweed ethanol extract contained alkaloids, flavonoids, saponins, steroids, and polyphenols and the IC50 value of red seaweed ethanol extract was 4.59 ppm, while the positive control for Vitamin C was 6.64 ppm. It was concluded that the ethanolic extract of red seaweed Galaxaura rugosa in the South Aceh District had high antioxidant potential, as evidenced by the small IC50 value of <50 µg/mL.Keywords: Antioxidants, Galauxara rugosa, phytochemicals, DPPH  ABSTRAKAktivitas antioksidan dari ekstrak etanol rumput laut merah Galaxaura rugosa Antioksidan merupakan senyawa yang mampu menunda, memperkecil, memperlambat atau menghambat reaksi oksidasi dari reaksi radikal bebas. Antioksidan menyumbangkan elektron kepada radikal bebas yang tidak stabil sehingga radikal bebas ini dapat dinetralkan agar tidak mengganggu jalannya proses metabolisme tubuh. Rumput laut merah Galaxaura rugosa berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan ekstrak etanol rumput laut merah Galaxaura rugosa terhadap radikal bebas DPPH berdasarkan nilai IC50. Pada penelitian ini dilakukan pengujian fitokimia dan penentuan aktivitas antioksidan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil) yang dilakukan secara kuantitatif menggunakan spektrofotometer UV-Vis. Hasil dari skrining fitokimia ekstrak etanol rumput laut merah mengandung senyawa alkaloid, flavonoid, saponin, steroid dan polifenol serta nilai IC50 ekstrak etanol rumput laut merah sebesar 4,59 ppm sedangkan kontrol positif Vitamin C sebesar 6,64 ppm. Ekstrak etanol rumput laut merah Galaxaura rugosa di Kabupaten Aceh Selatan memiliki potensi antioksidan yang tinggi. dibuktikan dengan nilai IC50 yang yang kecil yaitu < 50 µg/mL.Kata kunci: Antioksidan, Galauxara rugosa, Fitokimia, DPPH

AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN FRAKSI AKAR TABAR KEDAYAN (Aristolochia foveolata Merr.) DENGAN METODE DPPH (2,2 DIPHENYL-1-PICRILHYDRAZIL)

2019 ◽  
pp. 8-14
Author(s):  
Risa Supringrum ◽  
Siti Jubaidah
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
The Body ◽  
Hexane Fraction ◽  
High Dose ◽  
Water Fraction ◽  
Dpph Method

Antioxidants are substances that can neutralize free radicals, thus protecting the body from various diseases by binding to free radicals and highly reactive molecules that can damage cells. The use of high-dose synthetic antioxidants is reported to be toxic and carcinogenic. Tabar Kedayan root (Aristolochia foveolata Merr) is one of the native plants from North Kalimantan, which is empirically used as an anti-poison, containing secondary metabolites including alkaloids, tannins, flavonoids. Flavonoids are powerful antioxidants. Some studies report that the function of flavonoids can be to prevent and treat cancer. This study aims to determine the antioxidant activity of ethanol extract and n-hexane fraction, the ethyl acetate fraction of Tabar Kedayan root with 2.2 Diphenyl- 1-Picrilhydrazil (DPPH) method as a free radical compound. The results of the study obtained IC50 values for each sample, at ethyl acetate fraction 267.48 ppm, ethanol extract at 603.80 ppm, ethanol-water fraction 705.43 ppm, n- hexane fraction 1500 ppm. The antioxidant activity tests indicate that ethyl acetate fraction is categorized as weak antioxidant, while the ethanol-water fraction and n-hexane fraction exhibit no antioxidant activity. Keywords : Tabar Kedayan, Antioxidants, 2.2 Diphenyl-1-Picrilhydrazil

scholarly journals Skirining fitokimia ekstrak etanol kulit buah sirsak (Annona muricata Linn) dan uji aktivitas antioksidan dengan metode DPPH

2021 ◽  
Vol 2 (2) ◽  
pp. 60-64
Author(s):  
Nur Alim ◽  
Nurul Jummah ◽  
Agus Sangka Pratama ◽  
Nurdiyanti Nurdiyanti
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanol Extract ◽  
Skin Extract ◽  
Annona Muricata ◽  
Phytochemical Screening ◽  
Fruit Skin ◽  
Ic50 Value ◽  
Dpph Method

Phytochemical screening research on the ethanol extract of soursop (Annona muricata Linn) peel and antioxidant activity was tested using the DPPH method. The purpose of this study was to determine several classes of compounds found in soursop rind and to determine the antioxidant activity of ethanol extract of soursop rind against DPPH free radicals. The results showed ethanol extract of positive soursop fruit skin containing flavonoids, tannins, and terpenoids. of soursop fruit skin extract was carried out by DPPH method results obtained IC50 value of 192.13 µg / mL ± 5.198137 which was categorized as weak.

scholarly journals Antioxidant and Anti-Aging Activity of Green Mustard Ethanol Extract Gel

2021 ◽  
pp. 304-311
Author(s):  
Silvia Handayani ◽  
Edy Fachrial ◽  
Adek Amansyah ◽  
I. Nyoman Encrich Lister
Keyword(s):  
Antioxidant Activity ◽  
Oil Content ◽  
Ethanol Extract ◽  
The Body ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Ic50 Value ◽  
Dpph Method ◽  
Content Recovery ◽  
Better Than

The skin is the organ of the body that is frequently exposed to direct UV rays from the sun, which causes the formation of ROS (reactive oxygen species), leading to cell death and tissue damage. This health problem can be overcome by using antioxidants to stabilize the free radicals. Mustard as an organic substance which contains polysaccharide compounds, vitamin C, carotene, quercetin, routine compounds, kaempferol and β-cytostreol, all of these are beneficial to skin health and beauty. Therefore, this study aims to determine the antioxidant activity of the total flavonoids and phenol content of mustard ethanol extract using the DPPH method. Furthermore, the extract at concentrations of 2, 4, and 6% was also tested for its anti-aging activity using the following parameters: moisture and oil content, texture, collagen, wrinkles, pigment, sensitivity, and pores. The results showed that the antioxidant activity of the green mustard had an IC50 value of 170.7839 µg/ml, a total phenol content of 14.471 mg GAE/g extract and flavonoid content of 12.753 mg QE/g extract. The effectiveness of the aging activity of the extract was tested using formulation 6%, which is better than 2%, and 4%. Thus, the percentage of water content recovery, oil content, texture, collagen, wrinkles, stains, sensitivity and pores respectively was 36.41, 39.33, 64.07, 17.65, 65.28, 50.14, 49.10 and 35.09%.  Consequently, it was concluded that the extract has the potential of being developed into a herbal beauty product.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SPONS Stylissa sp. DENGAN MENGGUNAKAN METODE DPPH (1,1-difenil-2-pikrilhidrazil)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 419
Author(s):  
Samuel I.M. Sibarani ◽  
Adithya Yudistira ◽  
Deby A. Mpila
Keyword(s):  
Antioxidant Activity ◽  
Cell Wall ◽  
Free Radicals ◽  
Ethanol Extract ◽  
Dna Bases ◽  
Oxidation Reactions ◽  
Terrestrial Plants ◽  
Dpph Method ◽  
High Antioxidant Activity ◽  
Ethanol Extracts

ABSTRACTHabitat of sponge Stylissa sp., were under the sea and these sponges contain active compounds, which are more active than the compounds produced by terrestrial plants. Antioxidants are inhibitors of oxidation reactions due to the free radicals, which can cause weak damage to unsaturated cells, cell wall membranes, blood vessels, DNA bases, and lipid tissue, that causing disease. The study was conducted to determine the antioxidant activity of ethanol extracts  of sponge Stylissa sp., which is located on the Bangka Island, Likupang District, North Minahasa Regency. Sponge Stylissa sp., was extracted by maceration with using ethanol. Testing of antioxidant activity was carried out by the DPPH method measured by a UV-Vis spectrophotometer. The results of the study showed that ethanol extracts of sponge Stylissa sp., has antioxidant activity in each concentration and the highest at a concentration of 100 mg / L. The conclusion is a ethanol extract of sponge Stylissa sp. have high antioxidant activity with a concentration of 25 mg/L (77,40%), concentration of 50 mg/L (85,63%), concentration of 75 mg/L (88,66%), and concentration 100 mg/L (88,96%). Key words: Stylissa sp. Sponge, Antioxidant, DPPH (1,1-diphenyl-2-picrylhydrazyl) ABSTRAKHabitat dari spons Stylissa sp. terdapat di bawah laut dan spons ini mengandung senyawa aktif yang persentase keaktifannya lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Antioksidan adalah zat penghambat reaksi oksidasi akibat radikal bebas yang dapat menyebabkan kerusakan lemah tak jenuh, membran dinding sel, pembuluh darah, basa DNA, dan jaringan lipid sehingga menimbulkan penyakit. Penelitian dilakukan untuk mengetahui aktivitas antioksidan ekstrak etanol biota laut spons Stylissa sp. yang terdapat di pulau Bangka, Kecamatan Likupang, Kabupaten Minahasa Utara. Spons Stylissa sp. ini diekstrak dengan metode maserasi menggunakan etanol. Pengujian aktivitas antioksidan ini dilakukan dengan metode DPPH yang diukur dengan alat spektrofotometer UV-Vis. Hasil dari penelitian menunjukan bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan disetiap konsentrasi dan yang tertinggi pada konsentrasi 100 mg/L. Kesimpulan yang didapat bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan yang tinggi dengan konsentrasi 25 mg/L (77,40%), konsentrasi 50 mg/L (85,63%), konsentrasi 75 mg/L (88,66%), dan konsentrasi 100 mg/L (88,96%). Kata Kunci : Spons Stylissa sp., Antioksidan, DPPH (1,1-difenil-2-pikrilhidrazil)

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BULBUS BAWANG DAYAK (Eleutherine americana Merr) DENGAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 451
Author(s):  
Rizkah V. Mokoginta ◽  
Herny E. I. Simbala ◽  
Karlah L.R Mansauda
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Onion Plant ◽  
Dpph Method ◽  
Ic50 Values ◽  
Experimental Laboratory ◽  
Ethanol Extracts ◽  
Eleutherine Americana

ABSTRACTDayak onion plant (Eleutherine americana Merr.) or commonly called forest onion is a typical plant of Central Kalimantan that has been used by local peoples for cure a various types of diseases. This study aims to determine the activity of antioxidant compounds from Dayak bulb ethanol extracts from Kotamobagu City. This research method is an experimental laboratory. Initially the Dayak bulb sample was extracted maceration with 96% ethanol solvent then evaporated until a thick extract was obtained. As a parameter, the DPPH method (1,1-diphenyl-2-picrylhydrazyl) was used to test the antioxidant activity of the samples at concentrations of 50, 60, 70, 80 and 90 mg / L and as a comparison standard used Vitamin C with concentrations of 2, 4, 6, 8 and 10 mg / L. The results obtained showed antioxidant activity in Dayak bulb ethanol extracts with IC50 values of 41.46 mg / L and on vitamin C IC50 values of 1.04 mg / L. Based on these results it can be concluded that the onion bulb ethanol extract has very strong antioxidant activity. Keywords: Dayak Onion bulbs, Activity, Antioxidant, DPPH  ABSTRAK Tanaman bawang Dayak (Eleutherine americana Merr.) atau biasa disebut bawang hutan merupakan tanaman khas Kalimantan Tengah yang sudah dipergunakan masyarakat lokal sebagai obat berbagai jenis penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol bulbus bawang Dayak yang berasal dari Kota Kotamobagu. Metode penelitian ini adalah eksperimental laboratorium. Awalnya sampel bulbus bawang Dayak diekstraksi maserasi dengan pelarut etanol 96% kemudian dievaporasi hingga didapatkan ekstrak kental. Sebagai parameter, digunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl) untuk pengujian aktivitas antioksidan sampel pada konsentrasi 50, 60, 70, 80 dan 90 mg/L dan sebagai standar pembanding digunakan Vitamin C dengan konsentrasi 2, 4, 6, 8 dan 10 mg/L. Hasil penelitian yang didapat menunjukkan aktivitas antioksidan pada ekstrak etanol bulbus bawang Dayak dengan nilai IC50 sebesar 41,46 mg/L dan pada vitamin C nilai IC50 sebesar 1,04 mg/L. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bulbus bawang Dayak memiliki aktivitas antioksidan yang sangat kuat. Kata kunci: Bulbus Bawang Dayak, Aktivitas, Antioksidan, DPPH

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