scholarly journals Expression of KRAS in tissue samples of colorectal carcinoma and its correlation with various histo-pathological parameters.

2021 ◽  
Vol 29 (01) ◽  
pp. 94-100
Author(s):  
Hina Wasti ◽  
Beenish Hussain Nomani ◽  
Sehrish Shafique ◽  
Yumna Shahid ◽  
Hira Faisal
Keyword(s):  
Colorectal Carcinoma ◽  
Immunohistochemical Analysis ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Over Expression ◽  
Histological Variants ◽  
Dividing Cells ◽  
The Right ◽  
Advanced Tumors

Objective: To assess the expression of KRAS in tissue samples of colorectal carcinoma and to correlate it with histopathological parameters. Study Design: Cross Sectional study. Setting: Department of Pathology, PNS Shifa Hospital Karachi. Period: March 2016 to February 2019. Material & Methods: A total of 51 cases of CRC were analyzed for immunohistochemical staining using KRAS antibody on representative tissue blocks. Clinical and pathological records were retrieved for collection of data. The results of immunohistochemical analysis were correlated with the recorded clinico-pathological parameters. Results: In this study 51 cases of CRC were analyzed for immunoexpression of KRAS. The age of the patients ranged from 14 to 85 years with the mean age of 60.96 years. Among the 51 cases, 37(72.5%) cases were males and 14(27.4%) were females. 37(72.5%) were localized to left side colon and 14(27.4%) were found in the right colon. For KRAS immunostaining, 41(80.3%) out of 51 cases showed overexpression. Significant association was seen between KRAS overexpression and histological variants i.e. glandular carcinomas. Conclusion: In the present study over expression of KRAS was observed in advanced tumors. Majority of these cases were adenocarcinomas with few showed mucinous histology. The present study signifies that established KRAS expression is usually seen in rapidly dividing cells with association of advanced tumors.

scholarly journals HOME / ARCHIVES / VOL. 10 NO. 4 (2020): VOLUME 10 ISSUE 4 / Original Articles Expression of BRAF V600E in Tissue Samples of Colorectal Carcinoma and Its Correlation with Various Clinico-Pathological Parameters

2020 ◽  
Vol 10 (4) ◽  
pp. 271-276
Author(s):  
Hina Wasti ◽  
Summayyah Shawana ◽  
Beenish Hussain ◽  
Santosh Sidhwani ◽  
Rubbab Mir ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colorectal Carcinoma ◽  
Immunohistochemical Analysis ◽  
Tumor Location ◽  
Cross Sectional Study ◽  
Braf V600e ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Histological Variants ◽  
The Right

Objective: To determine the expression of BRAF V600E in tissue samples of colorectal carcinoma and to correlate it with various clinico-pathological parameters. Study design and setting: Cross-sectional study was conducted at department of Pathology, Pakistan Navy Station Shifa hospital Karachi from 1st March 2016 to 28th February 2019 Methodology: Total of 51 cases of colorectal cancer were analyzed for immunohistochemical staining using BRAF antibodies on representative tissue blocks. Clinical and pathological records were retrieved for data collection. The results of immunohistochemical analysis were correlated with the recorded clinico-pathological parameters. Results: In this study 51 cases of colorectal cancer were analyzed for immune expression of BRAF V600E. The age of the patients ranged from 14 to 85 years with the mean age of 60.96 years. Among the 51 cases, 37(72.5%) cases were males and 14(27.4%) were females. 37(72.5%) were localized to left side colon and 14(27.4%) were found in the right colon. For BRAF V600E, positive expression was seen in 20(39.2%) cases, whereas 31(60.7%) cases showed negative expression of BRAFV600E. No significant association was seen between BRAF V600E expression and histological variants like age, gender, tumor location and glandular carcinomas. Conclusion: BRAF V600E immunosuppression was seen in 39.2% of colorectal carcinoma in this study. No significant association was seen in BRAF V600E expression and histological variants

scholarly journals Lung weights and undiagnosed lung lesions in medico-legal autopsies in Dar es Salaam, Tanzania

2019 ◽  
Author(s):  
william mawalla ◽  
Henry A. Mwakyoma ◽  
Upendo Massamu
Keyword(s):  
General Population ◽  
Lung Diseases ◽  
Left Lung ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Heavy Burden ◽  
The Mean ◽  
Histopathological Studies ◽  
The Right

Abstract Background Medico-legal or forensic autopsy is a special type of autopsy performed under the orders of a legal authority in circumstances involving unnatural, suspicious or criminal deaths. Gross and histopathological studies of viscera in medico-legal autopsies have been observed to serve as crucial tool in the detection of undiagnosed disease conditions and the resulting findings may closely represent the general population. Methods A prospective cross sectional study was carried out at the Muhimbili National Hospital (MNH) department of pathology, to medico-legal autopsies received at the MNH mortuary. The study included 103 cases in an eight month period. Both lungs were weighed for each case and tissue samples were collected from each lobe, in both lungs. Other gross findings were observed and recorded. Tissue sections were made and stained with hematoxylin and eosin and evaluated under a microscope. Results Out of 103 cases studied, 88.3% were male with mean age of 35.8 + 16.6 years. In females, the mean age was 31.6 + 10.4 years. The mean weight of lungs was found to be 627.3 + 138.5g and 591.7 + 129.7g for the right and left lung, respectively, in males. In females, the mean weight of lungs was 616.7 + 166.1g and 583.3 + 155.8g for the right and left lung, respectively. More than half (53.4%) of lung samples had some pathological disease, and the leading diagnosis was pulmonary congestion/oedema (22.3%) followed by lobar pneumonia (18.5%). Age was found to relate with the observed histological diagnosis of the lungs. Conclusion Males, especially the youth and adults are the main victims of medico-legal deaths. The study also revealed high prevalence of undiagnosed lung diseases in victims of medico-legal deaths. These findings may reflect heavy burden of lung diseases in the general population.

scholarly journals HER2/Neu Expression was Different between Benign, Borderline and Malignant Epithelial Ovarian Tumors

2014 ◽  
pp. 46-49
Author(s):  
Yona S. Hadisubroto ◽  
Ketut Suwiyoga
Keyword(s):  
Ovarian Tumor ◽  
Cross Sectional Study ◽  
Ovarian Tumors ◽  
Chi Square ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Over Expression ◽  
Epithelial Ovarian Tumor ◽  
The Difference

Objective: To determine the difference of HER2/neu expression in benign, borderline and malignant epithelial ovarian tumor. Method: An analytic cross-sectional study was performed on 49 samples of epithelial ovarian cases which were divided into three groups: twelve cases of benign epithelial ovarian tumor, 9 cases of borderline epithelial ovarian tumor and 28 cases of malignant epithelial ovarian tumor. Paraffin embedded tissue samples was cut, stained with HER2/neu imunohistochemistry and examined in Pathology Laboratory at Sanglah Hospital. The other data was collected from medical record samples. The data was analysed using One Way Anova test and Chi-square test with level of confidence α = 0.05. Result: There were 13 cases (46.43%) of the HER2/neu over expression in malignant epithelial ovarian tumors, 2 cases (22.22%) of the HER2/neu over expression in borderline epithelial ovarian tumors and 1 case (9.09%) of the HER2/neu over expression in benign epithelial ovarian tumors. With p = 0.048 (p < 0.05), there are differentiation of HER2/neu expression in benign, borderline and malignant epithelial ovarian tumors. Conclusion: In this study HER2/neu expression was different between benign, borderline and malignant epithelial ovarian tumors. Keywords: benign epithelial ovarian tumor, borderline epithelial ovarian tumor, HER2/neu, malignant epithelial ovarian tumor, ovarian cancer.

Distribution and Characteristics of Intrathoracic Lymphadenopathy in TB/HIV Co-Infection

2019 ◽  
Vol 19 (4) ◽  
pp. 414-420
Author(s):  
Payam Mehrian ◽  
Abtin Doroudinia ◽  
Moghadaseh Shams ◽  
Niloufar Alizadeh
Keyword(s):  
Lymph Nodes ◽  
Cross Sectional Study ◽  
Short Axis ◽  
Mediastinal Lymph Nodes ◽  
Sectional Study ◽  
Cross Sectional ◽  
Immunodeficiency Virus ◽  
Hilar Adenopathy ◽  
The Right ◽  
Pulmonary Abnormalities

Background: Intrathoracic Lymphadenopathy (ITLN) in Human Immunodeficiency Virus (HIV) infected patients may have various etiologies and prognoses. Etiologies of ITLN can be distinguished based on the distribution of enlarged lymph nodes. Sometimes tuberculosis (TB) is the first sign of underlying HIV infection. Objective: We sought to determine ITLN distribution and associated pulmonary findings in TB/HIV co-infection using Computed Tomography (CT) scan. Methods: In this retrospective, observational, cross-sectional study, chest CT scans of 52 patients with TB/HIV co-infection were assessed for enlarged intrathoracic lymph nodes (>10 mm in short axis diameter), lymphadenopathy (LAP) distribution, calcification, conglomeration, the presence of hypodense center and associated pulmonary abnormalities. LAP distribution was compared in TB/HIV co-infection with isolated TB infection. Results: Mediastinal and/or hilar LAP were seen in 53.8% of TB/HIV co-infection patients. In all cases, LAP was multinational. The most frequent stations were right lower paratracheal and subcarinal stations. Lymph node conglomeration, hypodense center and calcification were noted in 25%, 21.4% and 3.5% of patients, respectively. LAP distribution was the same as that in patients with isolated TB infection except for the right hilar, right upper paratracheal and prevascular stations. All patients with mediastinal and/or hilar adenopathy had associated pulmonary abnormalities. Conclusion: All patients with TB/HIV co-infection and mediastinal and/or hilar adenopathy had associated pulmonary abnormalities. Superior mediastinal lymph nodes were less commonly affected in TB/HIV co-infection than isolated TB.

Disaster Risk Assessment Among Iranian Exceptional Schools

2021 ◽  
pp. 1-5
Author(s):  
Seyedeh Samaneh Miresmaeeli ◽  
Nafiseh Esmaeili ◽  
Sepideh Sadeghi Ashlaghi ◽  
Zahra Abbasi Dolatabadi
Keyword(s):  
Risk Assessment ◽  
Cross Sectional Study ◽  
Disaster Risk ◽  
Disaster Mitigation ◽  
Vulnerable Groups ◽  
Risk Level ◽  
Cross Sectional ◽  
Fire Extinguishing ◽  
Disaster Risk Assessment ◽  
The Right

Abstract Background: Exceptional children, like other children, have the right to be educated in a safe environment. Disasters are considered as serious issues regarding safety and security of educational environments. Following disasters, vulnerable groups, especially children with handicaps and disabilities are more likely to be seriously injured. Thus, the present study aimed to evaluate the safety and disaster risk assessment of exceptional schools in Tehran, Iran. Method: The cross-sectional study was conducted in exceptional schools in Tehran, 2018. First, 55 exceptional schools in all grades were selected based on census sampling method and evaluated by using a checklist designed by Tehran Disaster Mitigation and Management Organization (TDMMO) and Ministry of Education in 2015. The data were analyzed using Excel software and statistical descriptive tests. Result: Based on the results, school facilities are worn and have unsafe elevators (least safety: 7.69%), yards (least safety: 9.52%), laboratories (least safety: 16.67%), libraries (least safety: 24.24%), fire extinguishing systems (least safety: 28.99%), and storage rooms and kitchens (least safety: 33.33%) which require immediate considerations. In total, the safety of exceptional schools in this study was 70.13%, which suggests medium-risk level. Conclusion: The educational settings must be reconsidered, along with identifying the risk and safety at school. In addition, a standard should be established for evaluating safety, especially in exceptional schools.

Demonstration of the relationship between MSD and online learning during the COVID-19 pandemic

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Kochu Therisa Karingada ◽  
Michael Sony
Keyword(s):  
Online Learning ◽  
Undergraduate Students ◽  
Online Survey ◽  
Cross Sectional Study ◽  
The Body ◽  
Body Parts ◽  
Cross Sectional ◽  
Content Type ◽  
The Right ◽  
Almost All

PurposeThe COVID-19 pandemic lockdown has caught many educational institutions by surprise and warranted an abrupt migration from offline to online learning. This has resulted in an education change, without any time for due consideration, as regards its impact on musculoskeletal disorders (MSD) on students. The purpose of this study is to investigate MSD related to online learning during the COVID-19 pandemic lockdown.Design/methodology/approachA cross-sectional study was conducted on undergraduate students in India. In total, 261 students participated in this online survey.FindingsThe study finds that around 80% of students have reported some symptom in the head, neck and eyes since they started online learning. In total, 58% have reported MSD symptom in the right shoulder and 56% in the right hand fingers. Besides, more than 40 % of students experienced some MSD symptoms, in almost all the body parts studied, due to online learning. Correlation analysis is conducted between time spent on online learning per day and MSD symptoms.Originality/valueThis is the first study conducted on MSD and online learning during COVID-19 pandemic.

Peer effects in the valuation and practices of food safety: findings from the study of dairy consumers in India

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Raj Chandra ◽  
Abdul Munasib ◽  
Devesh Roy ◽  
Vinay K. Sonkar
Keyword(s):  
Social Networks ◽  
Food Safety ◽  
Peer Effects ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Content Type ◽  
Information Campaigns ◽  
Social Multiplier ◽  
Multiplier Effects ◽  
The Right

Purpose Information is often available to consumers through their social networks. Focusing on dairy consumers in India, this paper aims to present evidence of peer effects in consumers’ attitudes towards various food safety attributes and food safety practices. Design/methodology/approach Unobserved individual heterogeneities are crucial confounders in the identification of social (endogenous) effects. The identification is based on exploiting within-consumer variation across different aspects of attitude (or practices) related to food safety. Findings This paper uses a novel identification strategy that allows for average effects across attributes and practices to be estimated. Using the strategy, though this paper cannot estimate endogenous effects in each attribute or practice, this paper is able to identify such effects averaged over attributes or practices. Research limitations/implications Cross-sectional study, caste affiliation is not defined at the right level of granularity. Practical implications The results suggest that information campaigns aimed at creating awareness about food safety can have social multiplier effects, and this also translates into changes in the practices followed to mitigate food safety risks. Social implications In health-related awareness and practices, there are well-established cases of multiplier effects. The most significant example of this is the Pulse Polio campaign in India, where an awareness drives through social multiplier effects had such a significant impact that in 2012 India was declared polio-free. Perhaps, a similar campaign in matters related to food safety could be very fruitful. Originality/value The methodology and the issue are unique. Little exists in assessing social networks in the context of food safety.

Prevalence of Colorectal Carcinoma With Respect to Age and Gender

2021 ◽  
Vol 15 (12) ◽  
pp. 3198-3199
Author(s):  
Pervez Muhammad ◽  
Sajjad Muhammad ◽  
Muhammad Tariq ◽  
Amir Khan ◽  
Akhtar Munir ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Colorectal Adenocarcinoma ◽  
Cross Sectional Study ◽  
Medical Institute ◽  
Cross Sectional ◽  
Age And Gender ◽  
Medical College ◽  
Gender Study ◽  
The Mean ◽  
And Gender

Aim: Prevalence of colorectal carcinoma with respect to age and gender Study design: Observational / cross sectional study. Place and duration of study: This study was conducted at the Department of Pathology and Surgery, Post Graduate Medical Institute Lahore and Jinnah Medical College Peshawar from July, 2013 to February, 2015 Methodology: Surgical specimens of 60 histopathologically diagnosed cases of colorectal adenocarcinoma were included in this study. Age and sex were evaluated in CRC. Results: The mean±sd of age was 54.4±19.7 yrs. Out of 60 cases, 6 (10%) were between 16-29 yrs, 9 (15%) cases were between 30-49 yrs, 34 cases (56.7%) were of 50-69 yrs and 11 cases (18.33%) were of 70 yrs. Out of sixty cases, males were 33 (55%) and females were 27(45%). Conclusion: There are 34 out of 60(56.7%) patients were 50-69 years and 33 out of 60 (55%) cases were males i.e. males dominance. Keywords: Colorectal adenocarcinoma, immunohistochemistry, gender

scholarly journals Quantitative Assessment Of The Variations In Thyroid Dimensions In Relation To Increase In Serum TSH In Euthyroids

2018 ◽  
Vol 08 (02) ◽  
pp. 72-76
Author(s):  
Mahrukh Kamran ◽  
Sahar Mubeen ◽  
Iffat Raza ◽  
Sanobar Bughio ◽  
Hira Waseem ◽  
...  
Keyword(s):  
Total Variation ◽  
Cross Sectional Study ◽  
Thyroid Stimulating Hormone ◽  
Left Lobe ◽  
University Hospital ◽  
P Value ◽  
Cross Sectional ◽  
Right Lobe ◽  
The Right ◽  
Serum Tsh

Objectives: To determine the effect of serum TSH on thyroid dimensions of each lobe and to measure the amount of effect of per unit increase in serum TSH on thyroid dimensions of each lobe in euthyroids. Study Design: It was a cross-sectional study. Setting: The study was conducted at Ziauddin University Hospital, Clifton, Karachi. Methodology: Healthy participants aged 21 years and above were included through convenient sampling. Serum Thyroid stimulating hormone was evaluated and ultrasound of thyroid gland TG of 192 euthyroid participants was performed. Spearman correlation and regression analysis was applied to evaluate the relationship between TSH and TG dimensions Results: Relationship of increase in serum TSH with decrease in light lobe AP dimension was most significant. (r= -0.142 P-Value=0.001) and CC dimension least significant (r= -0.0098 P-Value=0.001). Where as in the left lobe AP dimension decreases significantly with increase serum TSH (r= -0.147 P-value=0.001). 11.7% of total variation in AP dimension, 3.5% of total variation in ML dimension and 6.5% of total variation in CC dimension in right lobe thyroid are because of serum TSH. While 9.5% of the total variation in AP dimension in left lobe is also due to serum TSH. Conclusion: Negative and significant correlation between serum TSH and thyroid dimensions was observed. Serum TSH inversely and significantly affects all the dimensions of the right lobe and only one dimension in the left lobe

scholarly journals Is stress an important co morbid factor for hypertension among legal professionals

2018 ◽  
Vol 5 (5) ◽  
pp. 1899
Author(s):  
L. Kannan ◽  
Praveena P.
Keyword(s):  
High Stress ◽  
Simple Random Sampling ◽  
Well Being ◽  
Cross Sectional Study ◽  
District Court ◽  
Cross Sectional ◽  
Legal Professionals ◽  
The Difference ◽  
The Right ◽  
Study Participants

Background: Stress is a mental, physical and emotional response to life demands. Long hours of work, client demands, changing laws- creates stress and eventually hypertension among advocates. Well-being of the advocates are being questioned. Hence the main objective of the study is to evaluate the relationship between stress and hypertension among practising advocates.Methods: A cross-sectional study was conducted in a sample of 300 practising advocates at Madurai district court for a period of 1 year by simple random sampling method.Results: Around 133 (44.33%) and 167 (55.66%) of study participants were in level of high stress and low stress respectively. Among high stressed participants, a majority of 113 (84.96%) were found to be hypertensives and among the low stressed participants only 10 (5.98%) were hypertensives. The difference of observation was found to be statistically significant (p=0.0000).Conclusions: In conclusion the study explains that practising advocates with high perceived stress 113 (84.96%) were found to be hypertensives. Stress has a significant association with hypertension. The right way to manage stress is to implement coping strategies.

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