scholarly journals Evaluation of anxiolytic potential of ethanol root extract and fractions of Pterocarpus mildbraedii in mice

2021 ◽  
Vol 17 (2) ◽  
pp. 086-093
Author(s):  
Kingsley Chimsorom Chilaka ◽  
Raymond Chidi Okonkwo ◽  
Malachy Ifeanyi Obi ◽  
Jane Ugochi Chilaka ◽  
Joseph Lanrewaju Oyindamola
Keyword(s):  
Laboratory Test ◽  
Crude Extract ◽  
Control Group ◽  
Active Principle ◽  
Root Extract ◽  
Test Model ◽  
Open Area ◽  
Traditional Use ◽  
Butanol Fraction ◽  
Significant Difference

To evaluate the anxiolytic potential of ethanol root extract and fractions of Pterocarpus mildbreadii in mice. Elevated-I-maze model apparatus is a straight wooden passage, divided equally (16cm each) into two enclosed areas (close arms) at both ends of the “maze” and an open area in the centre of two enclosed ends (arms). The ethylacetate fraction of Pterocarpus mildbraedii showed significant increase (p<0.05) in number of unprotected head dips (uHDIPS) when compared to control group but there was no significant difference when compared with other group. The butanol fraction of Pterocarpus mildbraedii showed significant increase in number of unprotected head dips (uHDIPS) at higher dose of 200mg/kg when compared to control group (p<0.05), there was no significant difference when compared with diazepam, crude extract, n-hexane, increased dose of butanol fraction (200mg/kg) there was increased significant difference. The crude extract of Pterocarpus mildbreadii showed significant increase in number of unprotected head dips (uHDIPs) at the dose of 100mg/kg, it also showed same significant increase in number of unprotected head dips with ethylacetate fraction at the dose of 200mg/kg. Ethylacetate fraction of Pterocarpus mildbreadii (200mg/kg) showed significant increase in number of unprotected head dips when compared with the crude extract at the dose of 100mg/kg (p<0.05).Diazepam (2mg/kg) showed significant increase in number of unprotected head dips when compared with the control group. Ethylacetate fraction of Pterocarpus mildbreadii (100mg/kg) showed significant increase (p<0.05) in number of protected head dips when compared with the control group. Increased dose of ethylacetate fraction of Pterocarpus mildbraedii (200mg/kg) showed significant increase (p<0.05) in number of protected head dipping when compared with the control group. The oral administration of ethylacetate fraction (100mg/kg) and 200mg/kg) to mice showed anti-anxiety effects indicated by increase in number of unprotected head dips and decrease in number of unprotected head dips.. Experimental evidence obtained in the laboratory test model could provide a rational for the traditional use of this plant. The plant can be further screened to evaluate and elucidate the mechanism of action and possibly isolate the active principle.

Valorization of the Pharmacological Potential of Phytochemical Compounds Contained in the Crude Extract of the Root of a Plant of Withania frutescens L

2019 ◽  
Author(s):  
A. EL Moussaoui ◽  
F. Jawhari ◽  
K. EL Ouahdani ◽  
I. Es-Safi ◽  
D. Bousta ◽  
...  
Keyword(s):  
Crude Extract ◽  
Control Group ◽  
Root Extract ◽  
Nacl Solution ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Pharmacological Potential

Our present study focuses on the evaluation of the analgesic, anti-inflammatory and healing activity of Withania frutescens L. The anti-inflammatory result has an inhibition percentage of 78.87% ± 7.08 at 450 mg/kg and 75.14% ± 6.39 at 400 mg/kg and 89.75% ± 3.44 for diclofenac (1%). When applied locally, the 10% cream has an inflammation inhibition of 96.87% ± 5.85 and 76.14% ± 7.88 for the 5% cream with 89.87 ± 6.20 of reference (Indomethacin). The abdominal contractions of rats treated with the root extract are significantly lower than those of the control group that received only physiological NaCl solution, with 41.20 ± 2.30 for the extract and 82.20 ± 5.04 for NaCl and 53.40 ± 4.94 for the reference. The healing activity of the studied extract records a percentage of contraction of about 93.20% ± 3.36 (Extract 10%), 84.50% ± 3.84 (Extract 5%), 48.47% ± 2.15 (control) and 81.88 ± 2.24 for the reference.

scholarly journals Screening of natural product extracts for fly repellent and larvicide

2021 ◽  
Vol 20 (1) ◽  
pp. 25-40
Author(s):  
Kanokwan Bootyothee ◽  
◽  
Surasit Aunpromma ◽  
Atchara Artchayasawat ◽  
Opal Pitaksakulrat ◽  
...  
Keyword(s):  
Natural Products ◽  
Mortality Rate ◽  
Crude Extract ◽  
Rural Areas ◽  
Inorganic Compounds ◽  
Food Contamination ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Significant Difference

Fly is one of the vectors of foodborne pathogenic vectors and causes myiasis in humans and animals. To prevent the contamination of food and myiasis, various chemical products are commonly used. However, eco-friendly is now a trend all over the world so natural products are the alternative ways to reduce the chemical residue in the environment. The present study was screened the 12 natural products in 4 groups; the essential oil plants, the alkaloid plants, the cyanogenic plant, and the inorganic compounds which use a simple extraction on the fly repelling and larvicide. The fly repelling efficacy with 2 criteria consist of the time of the first swarm and percent repelling (PR) in the first 15 min. The larvicidal efficacy was determined by the mortality rate within 24 h. The Kaffir lime peels aqueous crude extract exhibited the best result on fly repelling from both criteria with no significant difference. The bamboo shoots aqueous crude extract gave the best result on larvicide with significantly different from other groups (P < 0.001) which was faster than the positive control group (Coumaphos & Propoxur). Moreover, the high mortality rate reached 73.33 ± 15.28% within 24 h, higher than the positive control group. The present study indicates the power of common natural products on fly repellent and larvicide that may apply for fly repellant to reduce the food contamination especially fresh materials and prevention or treatment of animal and human myiasis in rural areas

scholarly journals A 90-Day Oral Toxicity of Hydroethanolic Root Extract of Carissa spinarum in Wistar Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Komlan M. Dossou-Yovo ◽  
Aboudoulatif Diallo ◽  
Povi Lawson-Evi ◽  
Yendubé T. Kantati ◽  
Tchin Darré ◽  
...  
Keyword(s):  
Body Weight ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Relative Weight ◽  
Control Group ◽  
Root Extract ◽  
Weight Changes ◽  
Oral Toxicity ◽  
Significant Difference

Background. Herbal medication is a worldwide and ancient practice, mostly in developing countries, where a large part of the population is involved in this practice. Hence, studies must be conducted to evaluate their safety and efficiency to avoid or prevent toxicological risks due to their usage. In Togo, Carissa spinarum is a medicinal plant belonging to Apocynaceae family, used as an aphrodisiac or to heal some ailments including malaria, sickle cell anemia, hypertension, pain, and asthma. Notwithstanding its several ethnomedicinal benefits, just a few toxicological data associated with its chronic use are available. Objective. Therefore, this study aims to assess the toxicity of an ethanolic root extract of Carissa spinarum in Wistar rats. Methods. The 90-day oral toxicity process following OECD TG 408 guidelines is used. Male Wistar rats received Carissa spinarum root hydroethanolic extract at 500 and 1000 mg/kg for 90 days by oral gavage. Body weight changes, hematological and blood biochemical parameters, organ weight changes, malondialdehyde as a lipoperoxidation marker expressed according to tissue proteins, and histopathology of vital organs were assessed. Results. No signs of toxicity or mortality were observed during the 90 days experiment. Hematological parameters have not shown any treatment-related abnormalities. According to biochemical parameters, an increase in the chloride ion level was observed at 1000 mg/kg p < 0.01 . There was no significant difference between the treated groups and the control group concerning the malondialdehyde concentration, body weight, and organ relative weight. No changes in necropsy and histopathology of vital organs associated with extract treatment were observed. Conclusion. The results indicated that an ethanolic root extract of Carissa spinarum does not cause adverse effects, which can lead to Wistar rats’ death after 90-day oral administration at 500 and 1000 mg.

scholarly journals Effect of piglet castration with nonsurgical sterilant on the zootechnical performance and pork carcass quality

2016 ◽  
Vol 68 (6) ◽  
pp. 1487-1496 ◽  
Author(s):  
D.S. Lucas ◽  
E.T.F. Siqueira ◽  
M.M.H. Haguiwara ◽  
S.S. Azevedo ◽  
S.E. Yotsuyanagi ◽  
...  
Keyword(s):  
Weight Gain ◽  
Meat Quality ◽  
Treated Group ◽  
Control Group ◽  
Active Principle ◽  
Carcass Quality ◽  
Feed Conversion ◽  
Zinc Gluconate ◽  
Hormone Levels ◽  
Significant Difference

ABSTRACT A non-surgical sterilant (NSS) was used with the objective of assessing the zootechnical performance, carcass and meat quality, and hormone levels. 90 male piglets were selected with birthweights varying between 1.5kg to 2.0kg. The control group was constituted of 45 males castrated by the conventional surgical method, at the seventh day of age, and the treated group was constituted of 45 males castrated with NSS (active principle of zinc gluconate in the concentration of 26.2mg/mL associated to the dimethyl sulfoxide at 0.5%), with the application of the first dose on the seventh day of age and the second dose on the fourteenth day of age. The zootechnical assessments were carried out on the farm and consisted: weight gain in the periods per animal and feed conversion. The slaughter of the animals and the assessment of the carcass and meat quality and hormone levels were carried out in a slaughterhouse and the municipality of Campinas (SP). The study demonstrated that the use of NSS was a viable alternative in relation to the zootechnical performance and the carcass quality. In relation to the meat quality there was no significant difference in the majority of the assessed parameters.

scholarly journals Evaluation of the comparison of anti-depressant effects of oral fluoxetine and riluzole in albino rats by using the forced swimming test model

2021 ◽  
Vol 10 (4) ◽  
pp. 391
Author(s):  
Hansraj Kumar ◽  
Akash Chandra ◽  
Uma Shankar Prasad Keshri ◽  
Rajiv Kumar
Keyword(s):  
Selective Serotonin Reuptake Inhibitors ◽  
Forced Swimming Test ◽  
Antidepressant Activity ◽  
Forced Swimming ◽  
Control Group ◽  
Albino Rats ◽  
Test Duration ◽  
Test Model ◽  
Significant Difference ◽  
Swimming Test

Background: Depression is a group of disorders results from a combination of multiple etiologic factors- genetic, biochemical, psychodynamic and socio-environmental. A depression consists of following clinical features as sadness, apathy, changes in sleep pattern, impaired concentration, feeling of shame or guilt and thoughts of dying or death. Fluoxetine and riluzole both are used for the treatment of depression in human being. Fluoxetine is SSRI (selective serotonin reuptake inhibitors) and riluzole is anxiolytic and mood stabilizer.Methods: Healthy male albino rats weighing between 150-200 grams were taken for the present study. Study animals were divided into three groups randomly with each group consisting of ten animals. Drugs were powdered with help of mortar and pestle and mixed in gum acacia solution. Appropriate volume of the freshly prepared solution was administered orally daily between 9 am to 10 am to all animal as per their individual body weight. Group A administered 1ml of 0.9% normal saline orally and serves as control group. Group B administered 0.4 mg of fluoxetine orally. Group C administered 2 mg of riluzole orally. Animals were evaluated for antidepressant activity using model- forced swimming test.Results: The results in the forced swimming test were assessed by duration of immobility in last 4 minutes of total 6 minute test duration. Antidepressant activity is indicated by the reduction in the duration of immobility i.e. lesser the duration more the efficacy. The results have been expressed as mean±standard deviation of duration of immobility in seconds during 6 minute period.Conclusions: There was significant difference in antidepressant activity of fluoxetine with antidepressant activity of riluzole. Riluzole showed antidepressant activity after two weeks of starting the drugs.

scholarly journals The effect of differentiated learning on the retainment scores of deep and surface learners

2015 ◽  
Vol 5 (2) ◽  
pp. 187-206 ◽  
Author(s):  
Serkan Demir ◽  
Mehmet Gürol
Keyword(s):  
Differentiated Instruction ◽  
Control Group ◽  
Test Model ◽  
Learning Approaches ◽  
Validity And Reliability ◽  
Process Learning ◽  
Significant Difference ◽  
The World ◽  
Differentiated Learning ◽  
5Th Grade

This study aims to determine the effect of tiered instruction and station techniques of differentiated instruction on students' achievements, learning approaches and learning retainment which are important in terms of learning-teaching process. Learning approaches which was one of the variables of the research is categorized as surface and deep according to information processing preferences of students. So the effect of the differentiated instruction on students' preferences was determined to be the aim of the study. The research was applied on the 5th grade students of two elementary school using pre, post and delayed test model. Both the experiment and the control group composed of 66 students. Differentiated instructions were prepared for the course model titled "Let's Learn the World of the Living" of Science and Technology course. An academic achievement test which was prepared by the researcher and other professionals, was tested in terms of validity and reliability. The test was applied before, after and four weeks later than the instruction. To determine the students' approaches, Learning Approaches Inventory was applied. In terms of achievement pre-test, significant difference is detected in favor of deep learner group. According to these findings, hypothesis is proven. In terms of achievement delayed-test, significant difference is detected in favor of deep leaner group. According to these findings, hypothesis is proven.

scholarly journals Evaluation of Antimalarial Activity of Methanolic Root Extract of Myrica salicifolia A Rich (Myricaceae) Against Plasmodium berghei–Infected Mice

2020 ◽  
Vol 25 ◽  
pp. 2515690X2092053 ◽  
Author(s):  
Zemene Demelash Kifle ◽  
Getnet Mequanint Adinew ◽  
Mestayet Geta Mengistie ◽  
Abyot Endale Gurmu ◽  
Engidaw Fentahun Enyew ◽  
...  
Keyword(s):  
Crude Extract ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Effective Vaccine ◽  
Root Extract ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
And Control

Background. The management and control of malaria has become gradually challenging due to the spread of drug-resistant parasites, lack of effective vaccine, and the resistance of vector to insecticides. Consequently, novel agents are urgently needed from different sources including from medicinal plants. In Ethiopia and Uganda, Myrica salicifolia root is traditionally claimed for the treatment of malaria. The aim of this study was to evaluate the in vivo antimalarial activity of root crude extract of M salicifolia. Methods. The parasite, Plasmodium berghei was used in this study since it is an appropriate parasite that is most commonly used because of its higher accessibility. A 4-day suppressive test was employed to evaluate the antimalarial effect of crude extract against early infection. The curative and prophylactic effect of the crude extract was further tested by Rane’s test and residual infection procedure. Parasitemia, survival time, packed cell volume, body weight, and rectal temperature of mice were used as evaluation parameters. Windows SPSS version 24 was used to analyze the data and analysis of variance followed by Tukey’s honestly significant difference to compare results between groups. Results. The root crude extract of M salicifolia significantly ( P < .05-.0001) suppressed parasitemia. The crude extract exhibited a chemosuppression of 40.90. Conclusion. The development of new antimalarial agents and the finding supports the traditional claims and previous in vitro studies.

scholarly journals Anthelmintic A-Type Procyanidins and Further Characterization of the Phenolic Composition of a Root Extract from Paullinia pinnata

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2287
Author(s):  
Verena Spiegler
Keyword(s):  
Crude Extract ◽  
Model Organism ◽  
Anthelmintic Activity ◽  
Root Extract ◽  
Traditional Use ◽  
Root Extracts ◽  
Traditional Remedies ◽  
Fast Centrifugal Partition Chromatography

Extracts from the roots of Paullinia pinnata L. are used in West Africa as traditional remedies for a variety of diseases including infestations with soil-transmitted helminths. Based on the results of an ethnopharmacological survey in Ghana, an aqueous acetone (70%) extract was investigated for its anthelmintic and phytochemical properties. Partitioning of the crude extract followed by several fractionation steps of the ethyl acetate phase using Sephadex® LH-20, fast centrifugal partition chromatography, RP-18-MPLC and HPLC led to isolation of six oligomeric A-type procyanidins (1 to 6). To determine the anthelmintic activity, the crude extract, fractions and isolated compounds were tested in vitro against the model organism Caenorhabditis elegans. A significantly better activity was observed for the trimeric A-type procyanidin (1) compared to a B-type trimer. However, this effect could not be generalized for the tetrameric procyanidins, for which the type of the interflavan-linkage (4→6 vs. 4→8) had the greatest impact on the bioactivity. Besides the procyanidins, three novel compounds, isofraxidin-7-O-α-l-rhamnopyranosyl-(1″→6′)-β-d-glucopyranoside (17), 4-methoxycatechol-2-O-(5′′-O-vanilloyl-β-apiofuranosyl)-(1′′→2′)-β-glucopyranoside (18) and a 6-(3-methoxy-4-hydroxyphenyl)-hexane-2,4-diol-2-O-hexoside (19) were isolated together with further ten known compounds (7 to 16), mainly coumarins and coumarinolignans. Except for 3-β-d-glucopyranosyloxy-4-methyl-2(5H)-furanone (15), none of the isolated compounds has previously been described for P. pinnata. The anthelmintic activity was attributed to the presence of procyanidins, but not to any of the other compound classes. In summary, the findings rationalize the traditional use of P. pinnata root extracts as anthelmintic remedies.

scholarly journals Study of the comparative effects of fluoxetine and ziprasidone in albino rats by using tail suspension test model

2020 ◽  
Vol 9 (1) ◽  
pp. 221
Author(s):  
Hansraj Kumar ◽  
Akash Chandra ◽  
Uma Shankar Prasad Keshari ◽  
Rajiv Kumar
Keyword(s):  
Selective Serotonin Reuptake Inhibitors ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Control Group ◽  
Albino Rats ◽  
Test Model ◽  
Tail Suspension ◽  
Significant Difference ◽  
Group B ◽  
Suspension Test

Background: Depression is a group of disorders results from a combination of multiple etiologic factors- genetic, biochemical, psychodynamic and socio-environmental. A depression consists of following clinical features as sadness, apathy, changes in sleep pattern, impaired concentration, feeling of shame or guilt and thoughts of dying or death. Fluoxetine and Ziprasidone both are used for the treatment of Depression in human being. Fluoxetine is Selective serotonin reuptake inhibitors (SSRI) and Ziprasidone is atypical antipsychotic.Methods: Healthy male albino rats weighing between 150-200 grams were taken for the present study. Study animals were divided into three groups randomly with each group consisting of ten animals. Drugs were powdered with help of mortar and pestle and mixed in gum acacia solution. Appropriate volume of the freshly prepared solution was administered orally daily between 9 am to 10 am to all animal as per their individual body weight. Group A administered 1ml of 0.9% normal saline orally and serves as control group. Group B administered 0.4 mg of fluoxetine orally. Group C administered 1.6 mg of ziprasidone orally. Animals were evaluated for antidepressant activity using two models – Tail suspension test and Forced swimming test.Results: The results in the tail suspension test were assessed by duration of immobility in 6 minutes duration. Antidepressant activity is indicated by the reduction in the duration of immobility i.e. lesser the duration indicates more effectiveness of the drug.Conclusions: There is significant difference in antidepressant activity of fluoxetine with antidepressant activity of ziprasidone.  

Sub-chronic toxicological evaluation of Strophanthus hispidus DC (Apocynaceae) aqueous root extract

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Muyiwa Samuel Fageyinbo ◽  
Abidemi James Akindele ◽  
Esther Oluwatoyin Agbaje
Keyword(s):  
Oral Administration ◽  
Liver Weight ◽  
Laboratory Animals ◽  
Control Group ◽  
Root Extract ◽  
Toxicological Evaluation ◽  
Toxicological Effect ◽  
Significant Difference ◽  
Haematological Analysis ◽  
Aqueous Root Extract

Abstract Objectives Strophanthus hispidus DC (Apocynaceae) has gained wide and extensive applications in herbal medicine in Africa for the treatment of quite a lot of diseases. Owing to the extensive application and the propensity of persistent consumption of this shrub, this research investigates the sub-chronic toxicological effect of aqueous root extract of S. hispidus (SHP) in laboratory animals (rats). Methods The rats were allotted into four groups of eight rats each (n=8) and orally treated daily for ninety (90) days with SHP extract at 100, 500 and 1,000 mg/kg and the control group received distilled water (10 mL/kg). The rats were weighed at 15 days interval. After 90 days daily oral administration of SHP extract, blood samples were collected from the rats into lithium heparin and EDTA bottles for biochemical and haematological analysis respectively. Vital organs were weighed and histological examination was performed on the liver and kidney Results The SHP extract displayed no significant (p>0.05) alterations in body weight of treated compared to control rats. At doses of 500 and 1,000 mg/kg, SHP-treated rats showed significant (p<0.05) increase in white blood cell (WBC), without significant difference in other haematological parameters. Non-significant (p>0.05) decrease in urea and non-significant (p>0.05) increase Na+, K+ and blood urea nitrogen (BUN) were observed. Significant (p<0.05) decrease in liver weight was observed without any alteration in the architecture of the liver and other organs investigated. Conclusions Aqueous root extract of S. hispidus demonstrated a good safety profile in rats. Therefore, S. hispidus is harmless and safe following sub-chronic oral administration.

Sign in / Sign up

Export Citation Format

Share Document