scholarly journals Effects of aerobic training on oxidative stress and inflammation in rats exposed to controlled emissions of carbon monoxide (CO) and nitrogen dioxide (NO2)

2020 ◽  
Vol 8 (2) ◽  
pp. 099-110
Author(s):  
Tito S. Albérick I ◽  
Nsompi Florent ◽  
Messan Folly ◽  
Kpatcha Tchazou ◽  
Lawani M. Mansourou ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Monoxide ◽  
Nitrogen Dioxide ◽  
Aerobic Exercise ◽  
Ambient Air ◽  
Repeated Exposure ◽  
The Other ◽  
Training Block ◽  
Other Hand ◽  
Tnf Α

The objective of this study is to determine the effects of isolated exercise and those of repeated exercise on oxidative stress and the inflammatory process following exposure to carbon monoxide (CO) and nitrogen dioxide (NO2). Material and Method: 15 male Wistar rats, all eight weeks old, were randomly assigned to three groups. CO and NO2 were produced by the combustion of diesel fuel using a device contained in a sealed metal box and supplied with ambient air by a pump. The ranges of CO and NO2 concentrations to which the rats were exposed ranged from 35 to 45 ppm and 0.2 to 0.3 ppm, respectively. The aerobic workouts were performed on a rat treadmill. Blood samples were taken 24 hours after completion of handling at D4, D9 and D14. Variables studied were Malondialdehyde (MDA) and Tumor Necrosis Factor-alpha (TNF-α). Results: Aerobic endurance training after repeated exposure to CO and NO2 induces at the end of the first training block (D4) a significant increase (p ˂ 0.01) in MDA and TNF-α. On the other hand, the second block (D9) and the third training block (D14) induced a significant decrease (p ˂ 0.01) in these same parameters. Conclusion: Aerobic exercise practiced in isolation exacerbates the oxidative stress and inflammation induced by exposure to CO and NO2. On the other hand, regular aerobic exercise in a less polluted environment, following five days of recovery, significantly reduces the high levels of oxidative stress and inflammation caused by repeated exposure to automobile pollutants.

Environmental and Hygienic Assessment of Ambient Air Pollution in Oil-Producing and Oil-Refining Areas of the Republic of Bashkortostan and Population Health Status

2020 ◽  
pp. 26-32
Author(s):  
Z.B. Baktybaeva ◽  
R.A. Suleymanov ◽  
T.K. Valeev ◽  
N.R. Rahmatullin ◽  
E.G. Stepanov ◽  
...  
Keyword(s):  
Air Pollution ◽  
Carbon Monoxide ◽  
Health Status ◽  
Nitrogen Dioxide ◽  
Population Health ◽  
Respiratory Diseases ◽  
Ambient Air ◽  
Oil Refining ◽  
Disease Rates ◽  
Total Suspended Particles

Introduction. High density of oil-producing and refining facilities in certain areas of Bashkortostan significantly affects the environment including ambient air quality in residential areas. Materials and methods. We analyzed concentrations of airborne toxicants (sulfur and nitrogen oxides, nitrogen and carbon oxides, hydrogen sulfide, ammonia, xylenes, toluene, phenol and total suspended particles) and population health status in the cities of Ufa, Sterlitamak, Salavat, Blagoveshchensk, and the Tuymazinsky District in 2007–2016. Pearson's correlation coefficients (r) were used to establish possible relationships between medico-demographic indicators and air pollution. Results. Republican fuel and energy enterprises contributed the most to local air pollution levels. Gross emissions from such enterprises as Bashneft-Ufaneftekhim and Bashneft-Navoil reached 43.69–49.77 thousand tons of pollutants per year. The levels of some air pollutants exceeded their maximum permissible concentrations. Elevated concentrations of ammonia, total suspended particles, nitrogen dioxide, and carbon monoxide were registered most frequently. High rates of congenital abnormalities, respiratory diseases in infants (aged 0-1), general mortality and morbidity of the population were observed in some oil-producing and refining areas. The correlation analysis proved the relationship between the concentration of carbon monoxide and general disease rates in adults based on hospital admissions (r = 0.898), general incidence rates in children (r = 0.957), and blood disease rates in infants (r = 0.821). Respiratory diseases in children correlated with nitrogen dioxide emission levels (r = 0.899). Conclusions. Further development of oil-producing, petrochemical and oil-refining industries should be carried out taking into account socio-economic living conditions of the population.

scholarly journals Are N-acetylcysteine and adalimumab effective for non-alcoholic steatohepatitis?

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Mustafa Yalçın ◽  
Muhammed Ali Kıyak ◽  
Mesut Akarsu ◽  
Aslı Çelik ◽  
Göksel Bengi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Treatment Options ◽  
Diet Group ◽  
Normal Diet ◽  
The Other ◽  
Albino Rats ◽  
Necrosis Factor Alpha ◽  
Alcoholic Steatohepatitis ◽  
Tnf Α ◽  
And Oxidative Stress

Due to the lack of effective medical treatment for non-alcoholic steatohepatitis (NASH), we aimed to evaluate new treatment options. In particular, our goal was to investigate and compare the effects of N-acetylcysteine (NAC) and Adalimumab treatment on tumor necrosis factor alpha (TNF-α) and oxidative stress during the development of NASH in a rat model of the disease. Our study included a total of 35 female Wistar albino rats that were divided into 5 groups of 7 each, and evaluated over a 6 week period. One group received a normal diet, while the other four groups received a methionine and choline deficient (MCD) diet. One of the groups receiving the MCD diet did not take any medicine, while the other three were administered NAC, adalimumab, or a NAC/adalimumab combination therapy. NASH was successfully established in the MCD diet group. Levels of TNF-α were effectively suppressed in the three groups that received therapy. Even though adalimumab significantly enhanced suppression of TNF-α, the NASH score was suppressed to a more statistically significant extent in the groups receiving NAC. Our study showed that TNF-α and oxidative stress play an important role in NASH pathogenesis. The antioxidant agent, NAC, was found to be superior to the anti-TNF agent, Adalimumab, in the improvement of total NASH score. Although these drugs did not prevent the development of NASH, it was shown that they mildly reverse the NASH histopathology score, suggesting improvement of and overall liver function.

scholarly journals Lycopene supplementation prevents reactive oxygen species mediated apoptosis in Sertoli cells of adult albino rats exposed to polychlorinated biphenyls

2013 ◽  
Vol 6 (2) ◽  
pp. 83-92 ◽  
Author(s):  
Gunasekaran Krishnamoorthy ◽  
Kandaswamy Selvakumar ◽  
Prabhu Venkataraman ◽  
Perumal Elumalai ◽  
Jagadeesan Arunakaran
Keyword(s):  
Oxidative Stress ◽  
Polychlorinated Biphenyls ◽  
Sertoli Cell ◽  
Sertoli Cells ◽  
Caspase 3 ◽  
Sperm Count ◽  
Reactive Oxygen ◽  
The Other ◽  
Sperm Production ◽  
Other Hand

Abstract Sertoli cell proliferation is attenuated before attaining puberty and the number is fixed in adult testes. Sertoli cells determine both testis size and daily sperm production by providing physical and metabolic support to spermatogenic cells. Polychlorinated biphenyls (PCBs) exposure disrupts functions of Sertoli cells causing infertility with decreased sperm count. On the other hand, lycopene is improving sperm count and motility by reducing oxidative stress in humans and animals. Hence we hypothesized that PCBs-induced infertility might be due to Sertoli cell apoptosis mediated by oxidative stress and lycopene might prevent PCBs-induced apoptosis by acting against oxidative stress. To test this hypothesis, animals were treated with vehicle control, lycopene, PCBs and PCBs + lycopene for 30 days. After the experimental period, the testes and cauda epididymidis were removed for isolation of Sertoli cells and sperm, respectively. We observed increased levels of oxidative stress markers (H2O2 and LPO) levels, increased expression of apoptotic molecules (caspase-8, Bad, Bid, Bax, cytochrome C and caspase-3), decreased anti-apoptotic (Bcl2) molecule and elevated apoptotic marker activity (caspase-3) in Sertoli cells of PCBs-exposed animals. These results were associated with decreased sperm count and motility in PCBs exposed animals. On the other hand, lycopene prevented the elevation of Sertoli cellular apoptotic parameters and prevented the reduction of sperm parameters (count and motility). The data confirmed that lycopene as an antioxidant scavenged reactive oxygen substances, prevented apoptosis, maintained normal function in Sertoli cells and helped to provide physical and metabolic support for sperm production, thereby treating infertility in men.

scholarly journals Does the Interdependence between Oxidative Stress and Inflammation Explain the Antioxidant Paradox?

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Subrata Kumar Biswas
Keyword(s):  
Oxidative Stress ◽  
Chronic Diseases ◽  
Inflammatory Process ◽  
The Other ◽  
Present Review ◽  
Low Grade ◽  
Inflammatory Pathways ◽  
Pathological Conditions ◽  
Other Hand ◽  
And Oxidative Stress

Oxidative stress has been implicated in many chronic diseases. However, antioxidant trials are so far largely unsuccessful as a preventive or curative measure. Chronic low-grade inflammatory process, on the other hand, plays a central role in the pathogenesis of a number of chronic diseases. Oxidative stress and inflammation are closely related pathophysiological processes, one of which can be easily induced by another. Thus, both processes are simultaneously found in many pathological conditions. Therefore, the failure of antioxidant trials might result from failure to select appropriate agents that specifically target both inflammation and oxidative stress or failure to use both antioxidants and anti-inflammatory agents simultaneously or use of nonselective agents that block some of the oxidative and/or inflammatory pathways but exaggerate the others. To examine whether the interdependence between oxidative stress and inflammation can explain the antioxidant paradox we discussed in the present review the basic aspects of oxidative stress and inflammation and their relationship and dependence.

scholarly journals The Role of Mitochondrial Impairment and Oxidative Stress in the Pathogenesis of Lithium-Induced Reproductive Toxicity in Male Mice

2021 ◽  
Vol 8 ◽  
Author(s):  
Mohammad Mehdi Ommati ◽  
Mohammad Reza Arabnezhad ◽  
Omid Farshad ◽  
Akram Jamshidzadeh ◽  
Hossein Niknahad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adverse Effects ◽  
Reproductive Toxicity ◽  
The Other ◽  
Mitochondrial Depolarization ◽  
Mitochondrial Impairment ◽  
Other Hand ◽  
Wide Range ◽  
The Impact ◽  
Testis Tissue

Lithium (Li+) is prescribed against a wide range of neurological disorders. Besides its excellent therapeutic properties, there are several adverse effects associated with Li+. The impact of Li+ on renal function and diabetes insipidus is the most common adverse effect of this drug. On the other hand, infertility and decreased libido is another complication associated with Li+. It has been found that sperm indices of functionality, as well as libido, is significantly reduced in Li+-treated men. These adverse effects might lead to drug incompliance and the cessation of drug therapy. Hence, the main aims of the current study were to illustrate the mechanisms of adverse effects of Li+ on the testis tissue, spermatogenesis process, and hormonal changes in two experimental models. In the in vitro experiments, Leydig cells (LCs) were isolated from healthy mice, cultured, and exposed to increasing concentrations of Li+ (0, 10, 50, and 100 ppm). In the in vivo section of the current study, mice were treated with Li+ (0, 10, 50, and 100 ppm, in drinking water) for five consecutive weeks. Testis and sperm samples were collected and assessed. A significant sign of cytotoxicity (LDH release and MTT assay), along with disrupted testosterone biosynthesis, impaired mitochondrial indices (ATP level and mitochondrial depolarization), and increased biomarkers of oxidative stress were detected in LCs exposed to Li+. On the other hand, a significant increase in serum and testis Li+ levels were detected in drug-treated mice. Moreover, ROS formation, LPO, protein carbonylation, and increased oxidized glutathione (GSSG) were detected in both testis tissue and sperm specimens of Li+-treated mice. Several sperm anomalies were also detected in Li+-treated animals. On the other hand, sperm mitochondrial indices (mitochondrial dehydrogenases activity and ATP levels) were significantly decreased in drug-treated groups where mitochondrial depolarization was increased dose-dependently. Altogether, these data mention oxidative stress and mitochondrial impairment as pivotal mechanisms involved in Li+-induced reproductive toxicity. Therefore, based on our previous publications in this area, therapeutic options, including compounds with high antioxidant properties that target these points might find a clinical value in ameliorating Li+-induced adverse effects on the male reproductive system.

Impact of Fuel Preparation System on Combustion Process and Exhaust Emissions

2003 ◽  
Author(s):  
S. Pischinger ◽  
M. Umierski
Keyword(s):  
Particulate Matter ◽  
Size Distribution ◽  
Diesel Fuel ◽  
Ambient Air ◽  
Common Rail ◽  
Sulphur Content ◽  
The Other ◽  
Injection Timing ◽  
Heavy Fuel Oil ◽  
Other Hand

In the past decade, the quality of Heavy Fuel Oil (HFO) has not improved or even declined in general. The progress in refinery techniques allowed to use a higher percentage of the crude oil for light fuels and other components. Therefore, the concentration of heavy components and agglomerates in the residual fuel increased. On the other hand, a better exhaust quality without compromising the superior fuel consumption of HFO-fuelled engines can only be gained by improved fuel injection. High injection pressures, rate shaping and electronic control of injection timing as well as new injection techniques like Common-Rail will be common features of engines for the next decades. Unfortunately, electronic controlled unit pumps and moreover, Common Rail systems are very sensitive to fuel quality, especially the content of water, sediments, agglomerates and other sludge. If ran on fuel processed by today’s fuel preparation system, wear would be excessive or damages will take place. On the other hand, if conventional on-board purification is intensified, the percentage of fuel deposit will rise and lower the over-all efficiency. To observe effects of fuel composition, a single cylinder research engine (250 mm bore/320 mm stroke) is fuelled by HFO in typical operation modes including different speeds and loads. Diesel fuel was also used to compare the results with another fuel of e.g. different sulphur content to separate effects on particulate matter. The high, typical sulphur content of the used HFO of about 3% increases the particulate matter; approx. 30% are sulphur products. The Soluble Organic Fraction (SOF, unburned fuel and oil) of PM is significantly higher compared to diesel fuel (50...70% to 10%). This impacts the particulate size distribution as well: although the main fraction of particulates consist of a size from 100...400nm for diesel and HFO, the number of small particulates (approx. 50 nm) is different, depending on the Sulphur content as this size consists of small condensates; therefore, the number may also be impacted by ambient air humidity. By use of different components for fuel preparation, the size, distribution and content of agglomerates can be impacted, but re-agglomeration occurs fast within minutes and must be taken into account. The differences in agglomerates does not result in a different total exhaust particulate mass. The slight effects found within the test are not significant and are in addition to that affected by ambient conditions. The fuel preparation, especially the use of homogenization equipment, needs more development efforts. For example, a mechanical homogenization apparatus can crack agglomerates and improve fuel homogeneity, but may fail to bring the size of agglomerates to a dimension that the filter is relieved.

The metacognitive awareness listening questionnaire as a metacognitive-raising tool: Does it work?

2015 ◽  
Vol 5 (1) ◽  
pp. 101 ◽  
Author(s):  
Rahman Sahragrard ◽  
Ali Kushki ◽  
M. Miri ◽  
Farzad Mahmooudi
Keyword(s):  
University Students ◽  
Repeated Exposure ◽  
Metacognitive Awareness ◽  
The Other ◽  
Control Group ◽  
Efl Learners ◽  
Other Hand ◽  
Post Test ◽  
And Control ◽  
Experimental Group

The present study aimed at exploring the influence of a repeated exposure to the Metacognitive Awareness Listening Questionnaire (MALQ) on EFL learners’ level of metacognitive awareness. Participants of the study were forty intermediate university students who were randomly assigned to experimental (n=20) and control (n=20) groups. The experimental group completed MALQ in odd sessions (seven sessions in total) across a semester. The control group, on the other hand, completed it in the first and last sessions of the course as pre-test and post-test, respectively. Results of the study showed that the questionnaire benefited the experimental group in a statistically significant way. Also, findings of the study revealed that less-skilled participants of the experimental groups benefited from the treatment more in comparison to their more-skilled counterparts in the experimental group. Keywords: MALQ, metacognitive instruction, metacognitive awareness

scholarly journals Upregulation of Peroxiredoxin 3 Protects Afg3l2-KO Cortical Neurons In Vitro from Oxidative Stress: A Paradigm for Neuronal Cell Survival under Neurodegenerative Conditions

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Barbara Bettegazzi ◽  
Ilaria Pelizzoni ◽  
Floramarida Salerno Scarzella ◽  
Lisa Michelle Restelli ◽  
Daniele Zacchetti ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cortical Neurons ◽  
Mitochondrial Dynamics ◽  
Neuronal Cell ◽  
The Other ◽  
Antioxidant Defenses ◽  
Glutathione Depletion ◽  
Other Hand ◽  
Peroxiredoxin 3

Several neurodegenerative disorders exhibit selective vulnerability, with subsets of neurons more affected than others, possibly because of the high expression of an altered gene or the presence of particular features that make them more susceptible to insults. On the other hand, resilient neurons may display the ability to develop antioxidant defenses, particularly in diseases of mitochondrial origin, where oxidative stress might contribute to the neurodegenerative process. In this work, we investigated the oxidative stress response of embryonic fibroblasts and cortical neurons obtained from Afg3l2-KO mice. AFG3L2 encodes a subunit of a protease complex that is expressed in mitochondria and acts as both quality control and regulatory enzyme affecting respiration and mitochondrial dynamics. When cells were subjected to an acute oxidative stress protocol, the survival of AFG3L2-KO MEFs was not significantly influenced and was comparable to that of WT; however, the basal level of the antioxidant molecule glutathione was higher. Indeed, glutathione depletion strongly affected the viability of KO, but not of WT MEF, thereby indicating that oxidative stress is more elevated in KO MEF even though well controlled by glutathione. On the other hand, when cortical KO neurons were put in culture, they immediately appeared more vulnerable than WT to the acute oxidative stress condition, but after few days in vitro, the situation was reversed with KO neurons being more resistant than WT to acute stress. This compensatory, protective competence was not due to the upregulation of glutathione, rather of two mitochondrial antioxidant proteins: superoxide dismutase 2 and, at an even higher level, peroxiredoxin 3. This body of evidence sheds light on the capability of neurons to activate neuroprotective pathways and points the attention to peroxiredoxin 3, an antioxidant enzyme that might be critical for neuronal survival also in other disorders affecting mitochondria.

scholarly journals Manganese-Induced Nephrotoxicity Is Mediated through Oxidative Stress and Mitochondrial Impairment

2020 ◽  
Vol 4 (2) ◽  
pp. 1-10 ◽  
Author(s):  
Amir Mohammad Niknahad ◽  
Mohammad Mehdi Ommati ◽  
Omid Farshad ◽  
Leila Moezi ◽  
Reza Heidari
Keyword(s):  
Oxidative Stress ◽  
Renal Injury ◽  
Kidney Tissue ◽  
Essential Element ◽  
The Other ◽  
Tubular Atrophy ◽  
Mitochondrial Impairment ◽  
Other Hand ◽  
Markers Of Oxidative Stress ◽  
Dehydrogenases Activity

Manganese (Mn) is an essential element that is incorporated in various metabolic pathways and enzyme structures. On the other hand, a range of adverse effects has been described in association with Mn overexposure. Mn is a well-known neurotoxic agent in mammals. Renal injury is another adverse effect associated with Mn intoxication. No precise mechanism for Mn nephrotoxicity has been identified so far. The current study was designed to evaluate the potential mechanisms of Mn-induced renal injury. Rats were treated with Mn (20 and 40 mg/mL, respectively, in drinking water) for 30 consecutive days. Markers of oxidative stress, as well as several mitochondrial indices, were assessed in the kidney tissue. Renal injury was evident in Mn-treated animals, as judged by a significant increase in serum BUN and creatinine. Moreover, urinalysis revealed a significant increase in urine glucose, phosphate, and protein in Mn-treated rats. Kidney histopathological alterations, including tubular atrophy, interstitial inflammation, and necrosis, were also detected in Mn-treated animals. Biomarkers of oxidative stress, including an increment in reactive oxygen species (ROS), lipid peroxidation, and oxidized glutathione (GSSG), were detected in Mn-treated groups. On the other hand, kidney glutathione (GSH) stores and total antioxidant capacity were depleted in Mn groups. Mn exposure was associated with significant mitochondrial depolarization, decreased mitochondrial dehydrogenases activity, mitochondrial permeabilization, and depletion of adenosine tri-phosphate (ATP) content. These data highlight oxidative stress and mitochondrial impairment as potential mechanisms involved in Mn-induced renal injury.

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