scholarly journals Biochemical parameters of mild, moderate & severe COVID-19 patients

2021 ◽  
Vol 11 (2) ◽  
pp. 031-039
Author(s):  
Khushbun Nahar Layla ◽  
Shahanara Yeasmin ◽  
Sharif Ahmed Khan ◽  
Masba Uddin Chowdhury ◽  
Afrina Binte Azad ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Serum Albumin ◽  
Prothrombin Time ◽  
Serum Ferritin ◽  
Biochemical Parameters ◽  
Rt Pcr ◽  
College Hospital ◽  
Positive Correlation ◽  
Medical College ◽  
D Dimer

Background and objectives: Coronavirus disease 2019 (COVID-19) is affecting millions of people world-wide. It is caused by the severe acute respiratory syndrome corona virus 2(SARS-CoV-2). The laboratory findings are very important to assess the progress of the disease. The present study is aimed to discuss the biochemical parameters among mild, moderate and severe COVID-19 patients. Materials and methods: A cross sectional study were conducted in the Department of Physiology, Dhaka Medical College, Dhaka from January 2020 to December 2020. After obtaining ethical clearance, a total of 100 real time-polymerase chain reaction (RT-PCR) COVID-19 positive patients were selected from Dhaka Medical College Hospital. With all aseptic precautions, 10 ml of venous blood was collected from ante-cubital vein. D-dimer, prothrombin time, C-reactive protein (CRP), lactate dehydrogenase (LDH), serum ferritin, random blood glucose (RBG), serum creatinine, serum glutamic-pyruvic-transaminase (SGPT) and serum albumin measured in the Department of Laboratory Medicine, Dhaka Medical College Hospital, Dhaka. CRP was measured by Immunoturbidimetric method, serum ferritin was estimated by Chemiluminescent microparticle immunoassay. STA-neoplastine CI plus used with STA-R analyzer was used for determination of prothrombin time. D-dimer was estimated by Immunofluorescence Assay method. Serum LDH is measured by Dimention clinical chemistry system, serum albumin is measured by bromocresol purple dye binding method, serum creatinine is measured by Jaffe alkaline picrate method and serum SGPT is measured by colourmetric (IFCC 1980) method and RBG is measured by enzymatic colorimetric method (GOD-PAP). Data were recorded in a pre-designed structured data collection form. For statistical analysis, ANOVA followed by Bonferroni test, Chi square test, Spearman’s rho correlation coefficient test was performed as applicable using SPSS for windows version 25.0. Results: By analyzing biochemical parameters of mild, moderate and severe RT-PCR positive 100 COVID-19 patients revealed evaluation of biochemical parameters shows severity of the disease was significantly associated with CRP, SGPT, S. Creatinine, LDH, Ferritin, D-dimer & Prothrombin time. No significant association was found with RBG & S. Albumin. Bonferroni correction following ANOVA was performed to compare between each group. Spearman’s correlation reveals statistically significant strong positive correlation with CRP, Ferritin & D-dimer, moderate positive correlation with S. Creatinine, LDH and mild positive correlation with SGPT & Prothrombin time. Conclusion: This study showed D-dimer, prothrombin time, CRP, LDH, ferritin, serum creatinine and SGPT are significantly associated with the severity of the illness that is higher in severe group in comparison to mild and moderate groups. So, comprehensive analysis of the biochemical parameters will be very helpful for early identification & better management of severe disease.

scholarly journals Assessment of Severity and Outcome of COVID-19 Cases by Haematological and Biochemical Markers at Tertiary Care Centre in India

2020 ◽  
pp. 196-207
Author(s):  
Amresh Kumar Singh ◽  
Jayesh Pandey ◽  
Indra Prasad Adhikari ◽  
Vivek Gaur ◽  
Ankur Kumar ◽  
...  
Keyword(s):  
Critically Ill ◽  
Serum Ferritin ◽  
Biochemical Parameters ◽  
Venous Blood ◽  
Tertiary Care ◽  
Rt Pcr ◽  
Biochemical Biomarkers ◽  
Corona Virus ◽  
The Mean ◽  
D Dimer

Background: In December 2019, a cluster of pneumonia cases caused by a novel corona virus (2019-nCov), later named as severe acute respiratory syndrome corona virus-2 (SARS-CoV-2) was detected in China. In India, 30th January 2020 first positive case ofSARS-CoV-2was reported from Kerala. The reverse transcriptase polymerase chain reaction (RT-PCR) is the standard method of choice for diagnosis of SARS-CoV-2 infection. Certain biomarker molecules that are being evaluated for assessment of severity and prognosis are; D-dimer, C reactive protein (CRP), lactate dehydrogenase (LDH), complete blood counts (CBC) and serum ferritin. The elevated levels of these biomarkers were associated with extent of inflammation. Objective: This prospective study was designed to assess the severity and prognosis of pneumonia cases caused by SARS-CoV-2 using different haematological and biochemical biomarkers. Materials and Methods: This study was conducted among 242 participants attending covid-19 facility of BRD Medical College Gorakhpur, after confirmation by RT-PCR. Different haematological and biochemical biomarkers were analyzed using 5 ml fasting venous blood samples and these were analysed in auto analysers using standard protocol as per manufacturer’s instructions. Finally result was analyzed using standard statistical calculation by %positivity, confidence interval, p values and ≤ 0.05 is considered as statistically significant. Results: Among a total of 242 COVID-19 cases based on different haematological and biomarkers assessment; 92 were critically ill and 150 non-critically ill. The mean ±SD of various haematological parameters among critically ill cases were; haemoglobin (13.0±1.8),TLC (13846.13±3903.76),PLT (92213.48±61415.07),NLR(36.5±30.4).The mean ±SD of the biochemical parameters of critically ill participant was; CRP 44.7±35.4 (95%CI 25.06,2.93),D. dimer 2.9±2.6 (95%CI 1.69,0.10), serum ferritin 1204.7±750.7 (95%CI 581.8,60.1),LDH 397.2±180.8 (95% CI 133.01,0.0163). The level of different haematological and biochemical parameters was raised also in non-critically ill cases but at lower side. Out of these 242 cases; 106 (43.8%) were died and 136 (56.2%) survived but the mortality was high in critically ill cases. Conclusion: Our findings show that level of D-dimer, LDH, CRP, NLR and serum ferritin,can be used to assess the severity and prognosis of COVID-19 cases. Among these biomarkers; D-dimer levels correlate more precisely with severity and can be considered as a reliable prognostic marker.

scholarly journals Evaluation of the performance of the COVID-19 rapid antigen tests in a tertiary level hospital in Bangladesh.

2021 ◽  
Author(s):  
Mohammad Jahidur Rahman Khan ◽  
Md. Shahadat Hossain ◽  
Samshad Jahan Shumu ◽  
Md. Selim Reza ◽  
Farzana Mim ◽  
...  
Keyword(s):  
Viral Load ◽  
Real Time ◽  
Sensitivity And Specificity ◽  
Detection Rate ◽  
High Sensitivity ◽  
Rt Pcr ◽  
College Hospital ◽  
Medical College ◽  
Qrt Pcr ◽  
Antigen Tests

Abstract Background: While the COVID-19 pandemic is a worldwide crisis, tests with high sensitivity and specificity are essential for identifying and managing COVID-19 patients. Globally, several rapid antigen tests RATs for COVID-19 have been developed, but their clinical efficacy has not been well established. This study aimed to evaluate the performance of several rapid antigen tests (RATs) to diagnose SARS-CoV-2 infection.Methods: This prospective observational study was conducted at Shaheed Suhrawardy Medical College hospital from February 2021 to April 2021 in Dhaka, Bangladesh. This study included the patients admitted in this hospital at the COVID-19 isolation unit or referred from the triage facility of the outdoor department of this hospital suspected as COVID-19 case. Two nasopharyngeal samples were collected simultaneously. one sample was used on the spot for the RAT. The other was sent to the adjacent Shaheed Suhrawardy Medical College COVID-19 RT-PCR laboratory for real-time reverse transcription-polymerase chain reaction (qRT-PCR). The performance of the RAT was evaluated using the results of qRT-PCR as a reference.Results: A total of 223 patients were included in this study, and the real-time RT-PCR detected SARS-CoV-2 in 84 (37.7%) patients. Of these 84 patients, 9 (10.7%) were asymptomatic. The overall sensitivity and specificity of RATs were 78.6% and 99.3%, respectively. The sensitivity was 81.3% in symptomatic cases and 55.6% in asymptomatic cases. False-negatives were observed in 18 patients, 3 of whom were asymptomatic and had a low viral load (cycle threshold (Ct) > 30). The detection rate of RATs was 100% when the Ct value was up to 24. The detection rate was 42.3% when the Ct was >29. The detection rate of RATs was 92.3% when the onset of symptoms was within three days. The detection rate was 33.3% when the onset of symptoms was >7 days.Conclusions: RATs for COVID-19 used in this study delivered an acceptable performance in patients with high viral load and within the first week of the onset of symptoms. They can be used as a supplementary method to RT-PCR for the diagnosis of COVID-19 patients.

scholarly journals Plasma D-Dimer Level in Different Types of Acute Ischaemic Stroke Patients: Experience at a Tertiary Hospital in Dhaka City

2019 ◽  
Vol 5 (1) ◽  
pp. 13-17
Author(s):  
Paritosh Kumar Sarkar ◽  
Anwar Israil ◽  
Mohammad Sayeed Hassan ◽  
Abu Nayeem ◽  
Md Azharul Hoque ◽  
...  
Keyword(s):  
Internal Medicine ◽  
Ischemic Stroke ◽  
Ischaemic Stroke ◽  
Clinical Diagnosis ◽  
Acute Ischaemic Stroke ◽  
Stroke Patients ◽  
College Hospital ◽  
Medical College ◽  
Different Types ◽  
D Dimer

Background: Plasma D-Dimer is a biomarker of thrombo-embolism. Objective: The purpose of the present study was to see the plasma D-dimer level in different types of acute ischaemic stroke patients. Methodology: This cross-sectional study was conducted in the Department of Neurology & Department of Internal Medicine at Dhaka Medical College Hospital, Dhaka, Bangladesh from July 2010 to June 2012 for a period of two (02) years. Patients with ischemic stroke with history within 7 days attending in the stroke clinic of Department of Neurology or admitted in the Department of Neurology and Internal Medicine through the outpatient and emergency Department of Dhaka Medical College Hospital (DMCH) were selected as study population for this study. Analysis of plasma D-Dimer was done in the Department of Hematology of DMCH.b Result: A total of 50 cases were recruited for this study. There were 24.0% lacunar infarcts, 40.0% atherothrombotic and 36.0% embolic infarcts in the study group. Highest level of plasma D-Dimer was observed in embolic (1700±964 ηg/ml) followed by atherothrombotic group (536±234 ηg/ml). The plasma D-Dimer was lowest (100±0 ηg/ml) in lacunar group. Concentration of Plasma D-Dimer showed significant correlation with clinical diagnosis in different subtypes of ischemic stroke (r=0.902; p=0.001) and also with risk factors, example, diabetes (r=0.319; p=0.012) and valvular heart disease (r=0.281; p=0.024), but no significant correlation with age, hypertension, hyperlipidaemia and smoking. Conclusion: In conclusion plasma D-Dimer is an important bio-marker in the clinical diagnosis and subtypes of ischemic stroke patients. Journal of National Institute of Neurosciences Bangladesh, 2019;5(1): 13-17

scholarly journals Association of Serum Ferritin Level with Acute Coronary Syndrome

2018 ◽  
Vol 32 (2) ◽  
pp. 89-93
Author(s):  
Abu Thaher Mohammad Mahfuzul Hoque ◽  
HI Lutfur Rahman Khan ◽  
Abdul Wadud Chowdhury ◽  
Md Mohshin Ahmed ◽  
Khandker Md Nurus Sabah ◽  
...  
Keyword(s):  
Acute Coronary Syndrome ◽  
Serum Ferritin ◽  
Serum Ferritin Level ◽  
Ferritin Level ◽  
Control Group ◽  
Case Group ◽  
Newly Diagnosed ◽  
College Hospital ◽  
Medical College ◽  
Coronary Syndrome

Background: A substantial number of patients get admitted in different hospitals of Bangladesh with the diagnosis of acute coronary syndrome (ACS). No underlying conventional risk factors can be identified in significant number of these patients. Therefore new emerging risk factors are likely to be involved in these patients. As many authors reported that high serum ferritin levels are associated with diabetes mellitus (DM) and hypertension (HTN), it may have role in the pathogenesis of ACS.So we designed this study to test the relation between hyperferritinemia and newly diagnosed acute coronary syndrome patients of Bangladesh.Methods: The study was an observational case control study done in Department of Cardiology, Dhaka Medical College Hospital, from January 2013 to December 2013. Newly diagnosed patients with acute coronary syndrome (ACS) in the age group of 30- 70 years, admitted in the coronary care unit (CCU) of Dhaka Medical College Hospital, Dhaka, within the study period were taken as cases and age& sex matched healthy subject with no history of ischemic heart disease (IHD) and with normal ECG were taken as control by purposive sampling. In our study, the number of cases and controls were 65 each. So, total number of subject was 130.Results: According to the serum ferritin level both cases and controls were divided into two sub groups: subjects with normal ferritin level and with hyperferritinaemia. Normal ferritin level was found in 35(53.8%) subjects of case group and 62(95.4%) subjects of control groups. Hyperferritinaemia was found in 30(46.2%) subjects of case group and 3(4.6%) subjects of control group. Hyperferritinaemia was found to be significantly higher in case group than in control group (p<0.001).Conclusion: The study concludes that the serum ferritin level of patients with ACS was significantly higher than the control group.Bangladesh Heart Journal 2017; 32(2) : 89-93

scholarly journals Iron Status in Pre-Eclampsia: A Case-Comparison Study

2013 ◽  
Vol 5 (2) ◽  
pp. 53-55 ◽  
Author(s):  
R Sultana ◽  
S Ahmed ◽  
N Sultana ◽  
SM Fazlul Karim
Keyword(s):  
Pregnant Women ◽  
Serum Ferritin ◽  
Serum Iron ◽  
Iron Status ◽  
Iron Concentration ◽  
College Hospital ◽  
Ferritin Concentration ◽  
Medical College ◽  
Serum Iron Concentration ◽  
Case Comparison

Preeclampsia is one of the most leading causes of maternal mortality in developing countries. Many studies have demonstrated the relation between alteration of iron status and preeclampsia. The aim of the study was to assess the status of iron in preeclampsia. This case control study was conducted in the Department of Biochemistry, Dhaka Medical College, Dhaka from July 2010 to June 2011. A total number of 100 pregnant women in the third trimester of pregnancy attending the Obstetrics & Gynaecology Department of Dhaka Medical College Hospital were selected purposively as study subjects. Among them 50 diagnosed cases of preeclampsia were selected as cases and 50 normal healthy pregnant women as controls. Serum ferritin and serum iron concentration were measured in all study subjects. The mean serum ferritin concentration in cases and controls were 95.06±50.07 ?g/l and 45.56±27.44 ?g/l respectively. Mean serum iron concentration in cases was 121.78±41.93 ?g/dl and that in controls was 61.04±24.18 ?g/dl. The present study showed significant differences of mean serum ferritin and serum iron between cases and controls. The study revealed presence of significantly higher level of serum ferritin and serum iron in preeclamptic group. DOI: http://dx.doi.org/10.3329/bjmb.v5i2.13345 Bangladesh J Med Biochem 2012; 5(2): 53-55

scholarly journals "Analysis of the Primary Presenting Symptoms and Hematological Findings of COVID19 Patients in Bangladesh"

2020 ◽  
Author(s):  
Abu Taiub Mohammed Mohiuddin Chowdhury ◽  
Md Rezaul Karim ◽  
H.M.Hamidullah Mehedi ◽  
Mohammad Shahbaz ◽  
Md Wazed Chowdhury ◽  
...  
Keyword(s):  
Prothrombin Time ◽  
Serum Ferritin ◽  
Clinical Symptoms ◽  
Presentation Rate ◽  
Moderate Degree ◽  
Presenting Symptoms ◽  
Global Pandemic ◽  
History Of ◽  
Higher Temperature ◽  
D Dimer

Abstract Background:SARS-Cov-2 infection or COVID-19 is a global pandemic. From the time of identification to till, multiple clinical symptoms and parameters have been identified by the researchers of various countries and regions regarding the diagnosis and presentations of COVID19 disease. In this manuscript, we investigated the primary symptoms and basic hematological presentations of SARS-CoV-2 infection among the Bangladeshi patients. Methodology: We have collected the disease history of mild to moderate degree of COVID19 patients; hematological and biochemical on admission reports of moderate degree COVID19 patients. All of them were tested positive for SARS-CoV-2 by RT PCR in different institutes in Bangladesh. Results: According to this study though COVID19 patients in Bangladesh commonly presented with fever, cough, fatigue, shortness of breath, and sore throat, but symptoms like myalgia, diarrhea, skin rash, headache, Abdominal pain/cramp, nausea, vomiting, restlessness, and a higher temperature of >1000F have a greater presentation rate and more frequent than other published studies. CRP and Prothrombin time was found to increase in all the patients. Serum ferritin, ESR, SGPT, and D-Dimer were found increased among 53.85%, 80.43, 44%, and 25% patients respectively. 17.39% of the patients had leucocytosis and neutrophilia. 28.26% of patients presented with lymphocytopenia. 62.52% of patients had mild erythrocytopenia. Conclusion: Despite some similarities, our study has evaluated a different expression in presenting symptoms in the case of COVID19 patients in Bangladesh. CRP, Prothrombin time, serum ferritin, ESR, SGPT, D-Dimer, erythrocytopenia, and lymphocytopenia can be initial diagnostic hematological findings and assessments for prognosis COVID19 disease. Also, Gender variation has a different scenario of clinical and laboratory appearance in this region.

scholarly journals Study on to assess pulmonary function test changes in asthmatic child using spirometry and its diagnostic and prognostic value

2017 ◽  
Vol 4 (3) ◽  
pp. 762
Author(s):  
Pratibha M. Patil ◽  
Manisha Chavan
Keyword(s):  
Pulmonary Function ◽  
Pulmonary Function Test ◽  
Asthmatic Child ◽  
Persistent Asthma ◽  
Research Centre ◽  
College Hospital ◽  
Positive Correlation ◽  
Medical College ◽  
Presenting Symptoms ◽  
Function Test

Background: Asthma is a disease that has become increasingly common over the last century making it now one of the commonest chronic disorders in the world. Spirometric lung function tests are playing a key role in the diagnosis and management of asthma in children. Considering the usefulness of spirometer in the diagnosis of asthma and scarcity of the literature regarding the role of spirometer in the diagnosis of Asthma especially in Indian context, the present study was undertaken to assess pulmonary function test changes in asthmatic child using spirometry and its diagnostic and prognostic value.Methods: The present study was conducted at Department of Paediatrics, Ashwini Rural Medical College, Hospital and Research Centre, Kumbhari during the study period of 2015 to 2016. Children presenting symptoms suggestive of asthma were included in the study.Results: In the present study, the commonest presentations included cough, breathing difficulty and recurrent wheeze in all the children (100%) followed by chest tightness (23%) and fever (11%). In the present study, the clinical and spirometry diagnosis of moderate asthma showed total positive correlation (100%). The positive correlation of mild persistent and intermittent asthma was limited to 97.30% and 91.18%. Severe persistent asthma positively correlated in 76.77%.Conclusions: It may be concluded that, the spirometry is not only helps to diagnose the asthma accurately, but also helps in assessing the severity which has the key role in the successful management. 

scholarly journals Role and Usefulness of C-reactive Protein in Women with Polycystic Ovarian Syndrome

2021 ◽  
pp. 64-72
Author(s):  
Indra Prasad Adhikari ◽  
Shreya Nigoskar ◽  
Amresh Kumar Singh ◽  
Arun Mishra
Keyword(s):  
Biochemical Parameters ◽  
Medical Condition ◽  
Polycystic Ovary ◽  
Density Lipoprotein ◽  
Low Grade ◽  
C Reactive Protein ◽  
College Hospital ◽  
Medical College ◽  
Reactive Protein ◽  
Serum Crp

Background: Polycystic ovary syndrome (PCOS) is a complex medical condition characterized by elevated androgen levels, menstrual irregularities, and small cysts on one or both ovaries. The prevalence of PCOS is 6 to 10% in women. C-reactive protein (CRP) is an acute-phase protein produced by the hepatic cells and it’s levels increased in inflammation that increases interleukin-6 by macrophages and T cells. Aims and Objective: A correlative study of role and usefulness of CRP in women with PCOS and its correlation with different biochemical parameters. Materials and Methods: This was hospital based case-control study carried out among PCOS was conducted in the Department of Obstetrics & Gynaecology, Index Medical College Hospital, Indore. This study was conducted from 1st January 2018 to 31st December 2019. A total of 260 subjects with age group between 15 to 45 years were divided into two group; cases (130) and controls (130). Results: Among a total of 260 cases, based on clinical and different biochemical parameters, 130 were diagnosis with PCOS and 130 were apparently healthy women. The mean ±SD of various parameters among PCOS cases were; body mass index (BMI) 32.97±8.466, P (<0.001) total cholesterol (TC) 188.42±31.126, P (<0.001), triglyceride (TG) 134.43±50.01, P (<0.001), high density lipoprotein (HDL) 36.29±9.55 TC/HDL ratio, 5.54±1.865 serum CRP, 3.41±0.94 versus BMI 22.87±2.470, P (<0.001), TC155.42±26.333, TG 110.00±42.19, HDL 41.22±10.912, TC/HDL ratio 4.08±1.39, serum CRP 2.25±0.83 P (<0.001) in healthy control. Conclusion: In this study, the role of inflammation and different biochemical markers were studied among PCOS. It was found that a majority of PCOS patients were obese, having insulin resistance. The levels of CRP as a marker of chronic low grade inflammation were higher in newly diagnosed PCOS as compared to the controls. The CRP values correlated well (statistically significant) with increased in BMI and age.

scholarly journals CORRELATION BETWEEN SERUM CREATININE & URINE ALBUMIN IN DIABETIC NEPHROPATHY PATIENTS: A STUDY IN A TERTIARY LEVEL HOSPITAL OF BANGLADESH

2019 ◽  
Vol 3 (8) ◽  
Author(s):  
Sanyal M ◽  
Khan A
Keyword(s):  
Diabetic Nephropathy ◽  
Serum Creatinine ◽  
Serum Creatinine Level ◽  
Creatinine Level ◽  
Total Protein ◽  
Medical College Hospital ◽  
Diabetic Patients ◽  
College Hospital ◽  
Medical College ◽  
Urine Albumin

Serum creatinine is a very common tool of renal assessment in diabetic nephropathy. Specially due to its availably and cost-effectiveness, serum creatinine has been a popular choice among the clinicians even in rural areas. Also, the staging of diabetic nephropathy is done on the basis of eGFR which is calculated mainly with serum creatinine level. Sometimes, clinicians , even overlook the other investigations ,such as urine routine examinations or ultra sonogram of kidney, when the serum creatinine level is within normal limit. This study aims at observation of existing correlation between serum creatinine and urine albumin in diabetic nephropathy patients. This cross sectional observational study has been conducted on 50 patients in a period of 6 months in the medicine department of Dhaka Medical College Hospital. 50 diabetic patients admitted to Dhaka Medical College Hospital were enrolled in this study after fulfilling inclusion and exclusion criteria. Among the total 50 patients, 34 patients were diagnosed on the basis of microalbuminuria and the rest had raised urinary total protein. No correlation was found between serum creatinine and urine albumin or urine total protein. Staging of the kidney disease was done according to eGFR (calculated by MDRD equation).  Only 41.37% of the patients with microalbuminuria, showed eGFR consistent with its staging while 31.5% of the patients with proteinuria showed respective expected eGFR. The rest of the patient’s eGFR was inconsistent with the staging. And the difference between the mean serum creatinine in two groups was insignificant. This study has shown that, the serum creatinine has no linear correlation with urinary albumin in diabetic nephropathy patients. So, commonly used serum creatinine based formula to calculate the eGFR can misinterpret the staging of the disease which can delay the appropriate treatment thereafter. Keywords: Diabetic Nephropathy, Proteinuria, Microalbuminuria, Serum Creatinine

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