scholarly journals Uji Efek Ekstrak Bawang Dayak (Eleutherine Americana Merr) Terhadap Motilitas Progresif 3 Spermatozoa

2021 ◽  
Vol 18 (2) ◽  
pp. 232
Author(s):  
NETI EKA JAYANTI ◽  
SITI RAUDAH ◽  
SUMIATI SUMIATI
Keyword(s):  
Lead Acetate ◽  
Lead Toxicity ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Spermatozoa Motility ◽  
Onion Extract ◽  
Testicular Spermatozoa

<p><strong>Abstract</strong></p><p><strong> </strong></p><p><strong>Background:</strong> <em>Dayak</em> onion extract can be used as antioxidants in reducing lead toxicity. This study aims to prove the potential of <em>dayak</em> onion in inhibiting decrease of motility testicular spermatozoa of  mice induced by lead acetate.</p><p><strong>Method:</strong> This study was a laboratory experimental study using Posttest Only Control Group Design design. The experimental animals were 30 mice (Mus musculus), divided into 5 groups. The K0 group was a negative control group receiving a Na-CMC 0,5% for 38 days at 0.1 ml. Day 1-3 K1 positive control group received Na-CMC 0,5%, K2-K4 group received <em>dayak</em> onion extract, K2 of 30 mg/kgBW, K3 60 mg/kgBW, and K4 120 mg/kgBW dose. Day 4-38 K1 group received 0.1 ml lead acetate, K2-K4 received lead acetate of 0.1 ml + <em>dayak</em> onion extract, K2 of 30mg/kgBW, K3 60 mg/kgBW, and K4 120 mg/kgBW as much as 0.2 ml.</p><p><strong>Result:</strong> There is a significant differences in spermatozoa concentration between K0 group with K3, K1 with K3 and K4, K2 with K3. Spermatozoa motility progressive 3 between K0 with K1, K2, K3, K4, K1 with K2, K3, K4 showed significant differences, while Testicular MDA content between K0 with K1, K2, K3 and K4, K1 with K0 and K3 showed significant differences.</p><p><strong>Conclusion</strong><strong>:</strong>  <em>Dayak</em> onion extract inhibit the decrease in spermatozoa concentration, inhibit the decrease in spermatozoa motility, and decrease testicular MDA level  in mice induced with lead acetate.</p><p> </p><p> </p><p><strong>Keyword</strong> : lead acetate,  <em>dayak</em> onion extract,  spermatozoa motility</p>

scholarly journals Effect of Chitosan on Histology of Reproductive Organs of Female Wistar Rats (Rattus norvegicus) Exposed to Acetate Lead

2019 ◽  
Vol 30 (4) ◽  
pp. 259
Author(s):  
Alief Ayu Purwitasari ◽  
Annisa' Wigati Rozifa ◽  
Dwi Dianita Irawan ◽  
Umi Kalsum ◽  
Retty Ratnawati ◽  
...  
Keyword(s):  
Treatment Group ◽  
Lead Acetate ◽  
Antral Follicle ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Female Rats ◽  
Control Group ◽  
P Value ◽  
Control Group Design

Lead is one of the pollutants widely spread in the environment because it is not easily decomposed. Lead can affect system functions such as the ovary and endometrium. Lead can trigger oxidative stress by reducing antioxidant enzymes and increasing Reactive Oxygen Species (ROS). Lead can also reduce Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) levels by disturbing the hypothalamus. Chitosan is an antioxidant compound that can reduce the toxic effects of lead. The purpose of this study was to study the effects of chitosan administration on the diameter of antral follicles, the number of endometrial arterioles, and the thickness of endometrial rats after lead acetate exposure. This study was an experimental laboratory using a posttest-only control group design approach applied on 25 female rats aged 8 weeks old, body weight 125-175 grams. Lead and chitosan were given orally with a sonde. There were 5 groups, namely, negative control group (without any treatment), positive control group (lead 175mg/kg/BW), treatment group 1 (lead 175mg/kg/BW + chitosan 16mg/kg/BW, treatment group 2 (lead 175mg/kg/BW + chitosan 32mg/kg/BW), and treatment group 3 (lead 175mg/kg/BW + chitosan 64mg/kg/BW) for 30 days. The rats were sacrificed at proestrus phase, which was proven from vaginal swab. Observations were carried out using the Hematoxylin Eosin (HE) staining method. The observations were analyzed using One Way ANOVA and followed by Least Significant Differences (LSD) test. The results showed significant results (p-value &lt;0.05). Chitosan can increase the diameter of the antral follicle, increase the number of endometrial arterioles, and increase the thickness of endometrial rats exposed by lead acetate.

scholarly journals Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

2018 ◽  
Vol 5 (1) ◽  
pp. 58
Author(s):  
Noer Kumala Indahsari ◽  
Masfufatun Masfufatun ◽  
Emilia Devi D.R
Keyword(s):  
Treatment Group ◽  
Moringa Oleifera ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
High Dose ◽  
Control Group Design ◽  
Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals An IGF-1 Levels Decrease in SOPK-RI Model Mice After A Low-Carb Diet

2020 ◽  
Vol 9 (2) ◽  
pp. 325-331
Author(s):  
Ermawati Ermawati ◽  
Budi Santoso ◽  
Widjiati Widjiati ◽  
Hany Puspita Aryani
Keyword(s):  
Design Research ◽  
Polycystic Ovary ◽  
Negative Control Group ◽  
Positive Control ◽  
Vaginal Swab ◽  
Negative Control ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Control Group Design

The aim of this study is to analyze the IGF-1 levels decrease in mice modeled by Polycystic Ovary Syndrome Insulin Resistance post carbohydrate high in protein. True Experimental Design with a Posttest Control Group Design research using 36 tails, female Wistar Rattus Norvegicus Strains, 3 months, 100-200 grams and randomly divided into 3 groups, namely K-(n =12, negative control group), K+(n=12, positive control group) and KP(n=12, treatment group). SOPK-IR modeling is done by mice in the injection of the hormone testosterone provionate at a dose of 100 mg/kg BW for 28 days, on the last day after injection a vaginal swab is performed to identify the condition of diestrus. The measurement of IGF-1 levels used the Enzyme-Linked Immunosorbent Assay method. Data analysis techniques used ANOVA test and LSD post hoc test. The results obtained mean levels of IGF-1 at K- (234.126±32.568) ng/mL, K+ (169.662±30.150) ng/mL, KP (146.528±29.562) ng/mL (p=0.000)

scholarly journals Pengaruh Pemberian Ekstrak Rosella (Hibiscus sabdariffa Linn) Terhadap Kadar Malondialdehid dan Aktivitas Katalase Tikus yang Terpapar Karbon Tetraklorida

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Zuraida Zuraida ◽  
Eti Yerizel ◽  
Eliza Anas
Keyword(s):  
Vitamin C ◽  
Negative Control Group ◽  
Organ Damage ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Hibiscus Sabdariffa ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Abstrak Pemberian rosella (Hibiscus sabdariffa Linn) diharapkan melindung hepar tikus dari kerusakkan akibat stres oksidatif pada keracunan karbon tetraklorida (CCl 4). Senyawa yang sering dijadikan petunjuk adanya kerusakan tersebut adalah malondialdehid (MDA). Rosella mengandung vitamin C, flavonoid, polifenol dan beta karoten. Tujuanpenelitian ini adalah menentukan pengaruh pemberian ekstrak rosella (Hibiscus sabdariffa Linn) terhadap MDA dan aktivitas katalase tikus yang terpapar CCl 4. Ini adalah penelitian eksperimental dengan desain Post test Only Control Group Design. Sampel 24 ekor tikus Strain Wistar berumur 2-3 bulan, berat 150-200 gr. Sampel diambil secara acakdan dibagi 4 kelompok terdiri dari kelompok kontrol negatif, kontrol positif (CCl 4), perlakuan 1 (CCl 4 dan ekstrak rosella 250 mg/kg bb) dan perlakuan 2 (CCl4  dan ekstrak rosella 500 mg/kg bb). Pemberian CCl 4secara oral dosis tunggal, setelah 24 jam kemudian diberi ekstrak rosella secara oral selama 14 hari. Data dianalisis dengan uji Anova, tingkatkepercayaan 95%.Pemberian ekstrak rosella secara statistik didapatkan perbedaan yang signifikan rerata kadar MDA dan katalase antar kelompok (p < 0,05). Disimpulkan bahwa ekstrak rosella dapat menurunkan kadar MDA dan meningkatkan aktivitas katalase tikus yang terpapar CCl 4. Kata kunci: karbon tetraklorida, MDA, katalase, rosella Abstract Administering roselle (Hibiscus sabdariffa Linn) is expected to protect rat liver from damage caused by oxidative stress in CCl4 poisoning. Rosella contains vitamin C, flavonoids, polyphenol and beta carotene. Compounds which was often used as marker of the damage caused by free radicals wa MDA. The objective of this study was to determine the effect of extracts of roselle (Hibiscus sabdariffa Linn) on MDA and catalase activity of rats exposed to CCl4. Experimental research design with Post test Only Control Group Design. Samples of 24 male Wistar Strain rats were 2-3 months old. weighing 150-200 gr. Samples were taken at random and divided into 4 groups consisting of a negative control group, positive control (CCl4), treatment 1 (CCl4 and roselle extract 250 mg / kg bw) and treatment 2 (CCl4 and roselle extract 500 mg / kg bw). CCl4 was given a single dose orally, after 24 hours, the subjects were given rosella extract orally for 14 days. Data were analyzed by ANOVA with a confidence level of 95%. Rosella extract obtained statistically significant differences of MDA and catalase levels among groups (p <0.05). It can be concluded that the rosella extract can reduce levels of MDA and increase the activity of catalase mice exposed to CCl4. From the research it can be concluded that the rosella extract can reduce levels of MDA and increas the activity of catalase mice exposed to CCl4. It is needed further research on the toxicity of extracts of rosella and organ damage caused.Keywords: carbon tetrachloride, MDA, catalase, rosella

scholarly journals Effectiveness of Spirulina platensis Extract on Wound Area and TNF-a Levels on Blood: Experimental Studies In Wistar Rats Made Artificially by Vulnus Scissum and Infected by Staphylococcus aureus

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Olvaria Misfa ◽  
Renni Yuniarti ◽  
Yan Wisnu Prajoko
Keyword(s):  
Wistar Rats ◽  
Experimental Studies ◽  
Serum Levels ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Wound Area ◽  
Post Hoc

This research uses randomizedpost-test only control group design. Thirty-two (32) male Wistar rats with incised skin and infected with S. aureusdivided into 4 groups, namely the group given S.platensisextract at a dose of 500 mg / kgBB / day (X1) and a dose of 750 mg / kgBB / day (X2 ), the negative control group was given saline solution (C1), and the positive control group was given amoxicillin 150 mg / kg orally(C2). Wound area measurements were taken on day 14 and serum TNF-? levels were examined on day 14 using the ELISA method. Data analysis was performed with one way ANOVAtestand continued with Post Hoc Test LSD.The results showed mean size of wound closure on the 14th day in groups X1, X2, C1 and C2 is 8095.74; 6270.98;15502.69; 11475.20micrometer with p <0.001. The mean TNF-? serum levels in the 14th day of X1, X2, C1, and C2 groups were 270.75; 222.83; 1730.33; 385.75pg / ml with p <0.001. Post Hoc Test of wound area showed significant differences between groups. Post Hoc Test TNF-? levels showed significant differences between treatment groups X1 and X2 with group C1.Spirulina platensisextract 500mg / kgBB / day and 750 mg /kgBB/ day have the smallest wound area significantly and reduce TNF-? levels on blood.

scholarly journals Potential of Cocoa Ethanol Extract Toward Nematocyst Tubul Firing Inhibition of Physalia Utriculus Toxin In Vitro

2019 ◽  
Vol 5 (2) ◽  
pp. 30
Author(s):  
Sarwendah Siswi Winasis ◽  
Al Munawir ◽  
Adelia Handoko
Keyword(s):  
Normal Control ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Average Percentage ◽  
Post Test

There was estimated 150 million envenomation cases due to jellyfish stings occur globally every year. 100 from 10,000 jellyfish species in the world known to be dangerous, one them is Physalia utriculus. The aim of this study was to determine the potential of cocoa (Theobroma cacao L.) ethanol extract toward nematocyst tubul firing inhibition of jellyfish (Physalia utriculus) toxin in vitro. The method was true experimental design with post test only control group design. The study divided into 8 groups: 1 normal control, 1 positive control, 1 negative control, and 5 treatments grups by giving cocoa ethanol extract with concentration 20%, 2%, 0.2%, 0.02%, 0.002%. The observation was made by calculating the percentage number of firing nematocysts. The result showed average percentage of firing nematocyst in the normal control group was 42.50 ± 3.18, positive control group was 37.97 ± 5.57, negative control group was 52.44 ± 2.98, and treatment group which given with cocoa ethanol extract 20%, 2%, 0.2%, 0.02%, 0.002% were 48.24 ± 5.37; 40.62 ± 7.10; 29.45 ± 5.39; 37.60 ± 9.78; 41.11 ± 3.92, respectively. The One Way Annova statistical results test show significance value 0.001 (p≤0.05). The conclusion of this study was the cocoa ethanol extract with concentration 0.2% has most potential to inhibit the jellyfish (P. utriculus) nematocyst tubule firing in vitro. Keywords: Physalia utriculus, cocoa, toxin, jellyfish

scholarly journals POTENSI EKSTRAK ETANOL BAWANG MERAH (Allium ascolonicum L.) DAN GARAM NaCl MENURUNKAN LUAS AREA SERTA MENINGKATKAN KONTRAKSI JARINGAN LUKA BAKAR RINGAN

Jurnal Kimia ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 53
Author(s):  
I M. Sukadana ◽  
S. Rahayu ◽  
Melli Melli
Keyword(s):  
Treatment Group ◽  
Subcutaneous Tissue ◽  
Healing Process ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Skin Damage ◽  
Control Group Design ◽  
Onion Extract ◽  
Skin Response

Burns is a skin response and subcutaneous tissue to temperature or thermal trauma that affects skin damage. The weight or severity of tissue damage from burns is influenced by several factors including deep burns, burns area, burn location, general body health, injury mechanism and age. The area of ??burns and the percentage of wound contractions is an indicator of the healing process of minor burns. This study used 25 wistar rats divided into 5 treatment groups with randomized posttest only control group design as follows; K: given vaseline treatment (negative control), Kp: given bioplacenton treatment (positive control), P1: given salt treatment at 7.5% concentration in vaseline, P2: given onion extract treatment at 40% concentration in vaseline, and P3: given a mixture of onion extract 40% and kitchen salt at a concentration of 7,5% in vaseline. The result of One-Way ANOVA and Post Hoc Test LSD using SPSS for Windows version 19 shows P2 treatment group is onion extract of 40% give the best result concentration of onion extract so it is very potential to be developed further as light burning agent compared to P1 and P3 treatment group because of its ability to decrease the area of ??340,79 mm2 on the first day to 11,75 mm2 and increased wound contraction by 81,59% at the end of treatment

scholarly journals Pengaruh gel ekstrak daun binahong (Anredera cordifolia) 5% terhadap peningkatan osteoblas pada proses penyembuhan luka pasca pencabutan gigi tikus strain WistarEffect of 5% binahong (Anredera cordifolia) leaf extract in increasing the osteoblast amount at the tooth extraction wound healing process of Wistar rat

2020 ◽  
Vol 32 (1) ◽  
pp. 9
Author(s):  
Jannatus Salis Sa'diyah ◽  
Dewi Ayu Septiana ◽  
Nanda Nailul Farih ◽  
Juwita Raditya Ningsih
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design

Pendahuluan: Pencabutan gigi di dunia kedokteran gigi adalah tindakan yang sering dilakukan. Pasca terjadinya pencabutan gigi, maka tulang pada soket akan rusak. Penyembuhan tulang ditandai dengan terbentuknya sel-sel osteoblas baru. Daun binahong merupakan daun yang mengandung flavonoid, alkaloid, terpenoid, asam askorbat, saponin, tanin dan asam oleanolik. Kandungan dari daun binahong dapat meningkatkan jumlah osteoblas pasca pencabutan gigi. Tujuan dari penelitian adalah menganalisis pengaruh pemberian gel ekstrak daun binahong (Anredera cordifolia) konsentrasi 5% terhadap peningkatan osteoblas pada proses penyembuhan luka pasca pencabutan gigi. Metode: Jenis penelitian ini merupakan penelitian eksperimental laboratorium murni dengan rancangan post test only control group design. Subyek penelitian berupa 45 ekor tikus dibagi menjadi 3 kelompok, yaitu kelompok gel ekstrak daun binahong, kontrol negatif dan kontrol positif. Gigi insisivus sentral kiri rahang bawah dilakukan pencabutan gigi kemudian diaplikasikan gel ekstrak daun binahong 5%, CMC-Na 1% dan iod gliserin. Tikus diterminasi pada hari ke 3,5,7,14 dan 21 pasca dilakukan pencabutan gigi untuk selanjutnya dibuat preparat histologis dengan pengecatan hematoksilin eosin. Jumlah sel osteoblas dihitung dengan optilab dan software image raster. Hasil: Hasil uji parametrik one way anova menunjukan terdapat perbedaan jumlah osteoblas yang bermakna antar kelompok (p<0,05) pada masing-masing hari. Hasil LSD (Least Significance Different) menunjukkan terdapat perbedaan yang bermakna (p<0,05) pada hari ke 5,7, 14 dan 21 antara kelompok perlakuan dengan kontrol negatif dan terdapat perbedaan yang bermakna pada hari ke 7 dan 14 antara kelompok perlakuan dan kontrol positif. Simpulan: Gel ekstrak daun binahong konsentrasi 5% berpengaruh dalam meningkatkan jumlah sel osteoblas pasca dilakukan pencabutan gigi.Kata kunci: Pencabutan gigi, gel ekstrak daun binahong, osteoblas. ABSTRACTIntroduction: Tooth extraction is the most common treatment in dentistry. After tooth extraction, the bone in the socket will be damaged. The formation of new osteoblast characterises bone healing. Binahong leaves contain flavonoids, alkaloids, terpenoids, ascorbic acid, saponins, tannins and oleanolic acids. This research was aimed to investigate the effectivity of binahong (Anredera cordifolia) leaf extract gel with a concentration of 5% in increasing the number of osteoblasts at the tooth extraction wound healing process. Methods: This research was an experimental laboratory with a post-test only control group design. Forty-five rats were divided into 3 groups; binahong leaf gel group, negative control group, and positive control group. Mandibular left central incisive was extracted, then binahong leaf gel, 1% CMC-Na, and glycerin were applied on the wound socket in different groups. All rats then terminated at day 3, 5, 7, 14, and 21 post-extraction and processed for the histological examination then coloured by haematoxylin-eosin. The number of osteoblasts was calculated by Optilab and raster image software. Results: The ANOVA test result showed significant differences in the number of osteoblasts among groups (p<0.05) on each day. The LSD (Least Significance Different) test result showed significant differences (p<0.05) at day 5, 7, 14, and 21 post-extraction, between binahong leaf gel group compared to the negative control group, and also at day 7 and 14 post-extraction between binahong leaf gel group compared to the positive control group. Conclusion: Binahong leaf extract gel with a concentration of 5% is effective in increasing the number of osteoblast in the wound healing process after tooth extraction.Keywords: Tooth extraction, Binahong leaf extract gel, osteoblast.

The Comparison of Ostecyte in the Pressure area and Tension area on Tooth Movement Because of Hyperbaric Oxygen Therapy

DENTA ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 34
Author(s):  
Arya Barahmanta ◽  
Muhammad Faizal Winaris ◽  
Pambudi Raharjo
Keyword(s):  
Hyperbaric Oxygen ◽  
Oxygen Therapy ◽  
Tooth Movement ◽  
Bone Remodelling ◽  
Orthodontic Tooth Movement ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Pressure Area

<p><strong><em>Background:</em></strong><em> Orthodontic tooth movement is a </em><em>interaction prosess</em><em> of resorption and deposition of bone remodeling. Orthodontic tooth movement by mechanical strength causes changes in alveolar bone. Osteocyte is an essential cell to respond bone remodelling. Hyperbaric Oxygen Therapy affects production of osteocyte because it can release Reactive Oxygen Species (ROS) and Nitrid Oxide (NO).  <strong>Purpose: </strong>To determine the difference number  of osteocyte in pressure and tension area during tooth movement by adjuvant of Hyperbaric Oxygen 2,4 ATA during 7 days starting on day 8 to day 14. <strong>Materials and Methods</strong>: This research used Completery Randomized Control Group Post Test Only Design. 36 cavia cobaya (male)  were divided into 3 groups randomly : the negative control groups, positive control group, and treatment group. Preparat staining used Hematoxylin Eosin (HE) and calculated on microscop 1000x with 20 field of view. Data analyses used one way ANOVA and LSD test then compared each area by using paired T test. <strong>Result:</strong> The data showed that the treatment group (P=10,67) tension area has the highest number of osteocyte than  negative control group (K-=3,67), positive control (K+=7,42). In the pressure area showed that negative control group (K-=5,00) has the highest  than positive control group (K+=3,83) and treatment (P=3,25). <strong>Conclusion: </strong>Therapy HBO 2,4 ATA 7 days starting on day 8 to day 14 is could increase osteocyte in the tissue to stimulate process of bone remodelling.</em></p><pre><strong> </strong></pre><p><strong><em>Keywords:</em></strong><em> Hyperbaric Oxygen, Tooth movement, Bone remodeling, </em><em>Osteocyte</em><em></em></p><p><em> </em></p><p><strong><em>Correspondence:</em></strong><em> </em><em>Arya Brahmanta</em><em>, Department of Orthodonty, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email:</em><em> </em><a href="mailto:[email protected]"><em>arya.brahmanta</em><em>@</em><em>hangtuah.ac.id</em></a></p>

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