Effect of  〖ZrO〗_2 and Y_2 O_3 Deposition on Biological Behavior of Ti-Base Alloys

Titanium has a good ability to attach to bone and living tissue, making it a perfect material for orthopedic implants. Because of the combination of high resistance to corrosion, biocompatibility and excellent mechanical properties. This work aims to study the Modifications of various base titanium implant samples producing by using powder technology (Ti-pure, Ti-45 % Ni, Ti10 % Co, and Ti-30 % Ta) by deposition of Nano Zirconia and yttria powders (70 % and 30% ). Chemical pretreatment carried out to prepare the implant surface before deposition, while the deposition process accomplished by pack cementation. The Characterizations of samples accomplished before and after the surface treatment, which includes: microstructure observation, x-ray diffraction (XRD), MTT Assay (cell viability) and MTT assay (cell adhesion). From the SEM All samples Show that Nano Zirconia and yttria were homogeneously put on the surface and fully covered it which resulted in a substantial modification in surface morphologies. From XRD patterns the peaks slightly shifted to the low angle side also amorphous behavior was observed. From MTT graphs it was found that the titanium alloys surface after pack cementation became more active after 3 days of exposure in MG-63 cells and there was a remarkable increase in cell viability and cell attachment compared with untreated samples.

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Effect of two commercial acellular dermal scaffolds on biological behavior of human gingival fibroblasts

Journal of Advanced Periodontology & Implant Dentistry ◽

10.34172/japid.2020.011 ◽

2020 ◽

Vol 12 (2) ◽

pp. 85-90

Author(s):

Omid Moghaddas ◽

Behdokht Miremadi ◽

Ehsan Seyed Jafari

Keyword(s):

Cell Viability ◽

Mtt Assay ◽

Cell Attachment ◽

Periodontal Regeneration ◽

Human Gingival Fibroblasts ◽

Biological Behavior ◽

Gingival Fibroblasts ◽

Dermal Matrix ◽

Acellular Dermal

Background. Periodontal regeneration is an essential goal of periodontal therapy. Acellular dermal matrix (ADM) has been recommended as an alternative to autogenous grafts. However, since it is devoid of cells and vasculature, there are concerns regarding the biological behavior of cells on ADM. This study aimed to assess the effects of two commonly used ADMs on biological behavior, i.e., attachment and proliferation, of human gingival fibroblasts (HGFs). Methods. This in vitro, experimental study was conducted on explanted and cultured HGFs. ADM types 1 and 2 (n=26; measuring 10×15 mm) were rinsed with saline solution, adapted to the bottom of 52 wells, exposed to HGFs with a cell density of 16,000 cells/mL, and incubated at 37°C for 12, 24, and 84 hours and seven days. Cell attachment was assessed 12 hours after incubation using 4›,6-diamidino-2-phenylindole (DAPI) and methyl-thiazol-diphenyl-tetrazolium (MTT) assay under a fluorescence microscope. Cell viability was assessed at 24 and 84 hours and one week using the MTT assay. Cells were then platinum-coated, and their morphology was evaluated under a scanning electron microscope (SEM). Data were analyzed using ANOVA. Results. HGFs were evaluated in 60 samples in three groups (n=20). Cell attachment was the same in the three groups, as shown by the MTT assay and DAPI test (P=0.6). Cell viability at one week was 3.73±0.02, 2.88±0.29, and 2.13±0.24 in the control, ADM 1, and ADM 2 groups, respectively. The difference was statistically significant (P=0.01). Conclusion. Both scaffolds were the same in terms of attachment of HGFs. However, ADM 1 was superior to ADM2 in terms of cell viability and morphology at one week. It was concluded that the quality of acellular dermal scaffolds could significantly influence cellular behaviors and tissue maturation.

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Re-establishment of Biocompatibility of the In Vitro Contaminated Titanium Surface Using Osteoconductive Powders With Air-Abrasive Treatment

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-17-00128 ◽

2018 ◽

Vol 44 (2) ◽

pp. 94-101 ◽

Cited By ~ 2

Author(s):

Ceylin S. Tastepe ◽

Xingnan Lin ◽

Marcel Donnet ◽

Behrouz Zandieh Doulabi ◽

Daniel Wismeijer ◽

...

Keyword(s):

Cell Viability ◽

Cell Attachment ◽

Treatment Modalities ◽

Dna Amount ◽

Implant Surface ◽

Abrasive Treatment ◽

Significant Difference ◽

Proliferation And Differentiation ◽

Film Layer

To achieve re-osseointegration on implant surfaces exposed to peri-implant infections, treatment should re-establish biocompatibility. The aim of this study was to test whether air powder abrasive treatment (APA) using osteoconductive powders can, in addition to cleaning, increase the biocompatibility of the contaminated implant surface. Ninety-six in vitro Ca-precipitated, organic film layer–coated sandblasted and acid-etched titanium discs were treated by APA using erythritol, hydroxylapatite (HA), and biocalcium phosphate (BioCaP) powders (n = 16 per group). Six treatment modalities were created (HA or erythritol cleaning with/without BioCaP coating). MC3T3-E1cells were seeded on discs, and cell attachment, viability, proliferation, and differentiation were evaluated. Pristine discs were used as control (control 1). Contaminated and nontreated discs were used as control (control 2). The cells were stretched and attached in all test groups. The cell viability and proliferation (DNA amount) in all test groups were significantly higher than in the pristine and contaminated disc groups. There was no significant difference between the test groups. The differentiation (alkaline phosphatase activity) of the cells on treated discs was significantly higher than on the contaminated discs but lower than in the pristine group. The cell viability in control 2 was significantly lower than the control 1. The APA with osteoconductive powder on contaminated titanium surfaces promoted the cell viability, proliferation, and differentiation of the MC3T3-E1 cells. The biocompatibility of the surface was higher than that of the contaminated discs. The tested aspects of cell response, with the exception of differentiation, reached to the level of the pristine surface. The in vitro results showed that APA with osteoconductive powders could be a promising method for implant surface treatment.

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Bioactive Sphene-Based Ceramic Coatings on cpTi Substrates for Dental Implants: An In Vitro Study

Materials ◽

10.3390/ma11112234 ◽

2018 ◽

Vol 11 (11) ◽

pp. 2234 ◽

Cited By ~ 11

Author(s):

Hamada Elsayed ◽

Giulia Brunello ◽

Chiara Gardin ◽

Letizia Ferroni ◽

Denis Badocco ◽

...

Keyword(s):

Osteogenic Differentiation ◽

In Vitro Study ◽

Titanium Implant ◽

Orthopedic Implants ◽

Ceramic Coatings ◽

Immunofluorescent Staining ◽

Implant Surface ◽

Osteogenic Differentiation Medium ◽

Alizarin Red

Titanium implant surface modifications have been widely investigated to favor the process of osseointegration. The present work aimed to evaluate the effect of sphene (CaTiSiO5) biocoating, on titanium substrates, on the in vitro osteogenic differentiation of Human Adipose-Derived Stem Cells (hADSCs). Sphene bioceramic coatings were prepared using preceramic polymers and nano-sized active fillers and deposited by spray coating. Scanning Electron Microscopy (SEM) analysis, surface roughness measurements and X-ray diffraction analysis were performed. The chemical stability of the coatings in Tris-HCl solution was investigated. In vitro studies were performed by means of proliferation test of hADSCs seeded on coated and uncoated samples after 21 days. Methyl Thiazolyl-Tetrazolium (MTT) test and immunofluorescent staining with phalloidin confirmed the in vitro biocompatibility of both substrates. In vitro osteogenic differentiation of the cells was evaluated using Alizarin Red S staining and quantification assay and real-time PCR (Polymerase Chain Reaction). When hADSCs were cultured in the presence of Osteogenic Differentiation Medium, a significantly higher accumulation of calcium deposits onto the sphene-coated surfaces than on uncoated controls was detected. Osteogenic differentiation on both samples was confirmed by PCR. The proposed coating seems to be promising for dental and orthopedic implants, in terms of composition and deposition technology.

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Observation of Proliferation and Attachment of Three Different Cell Types on Nano-Fibrous Silk Film & Scaffold

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.342-343.85 ◽

2007 ◽

Vol 342-343 ◽

pp. 85-88 ◽

Cited By ~ 1

Author(s):

Hyun Sook Baek ◽

Young Hwan Park ◽

Ki Chang Seok ◽

Jong Chul Park ◽

Don Kyun Rah

Keyword(s):

Alkaline Phosphatase ◽

Cell Viability ◽

Phosphatase Activity ◽

Silk Fibroin ◽

Alkaline Phosphatase Activity ◽

Mtt Assay ◽

Cell Attachment ◽

Three Dimensional ◽

Cell Types ◽

Different Cell Types

Attachment and viability of different cell types(fibrioblast, chondrocyte and osteoblast ) was observed on two forms of silk (mat & Three-dimensional scaffolds). The osteoblasts behaviors cultured on silk mat were significantly higher than that found on 3-D silk fibroin scaffold (3-D SF scaffold). In the MTT assay, the cell viability of fibroblasts, chondrocyte and osteoblasts seeded on 2-D nanofiber mat was (2-D mat) significantly higher than that found on 3-D SF scaffold. Similar result could be seen from SEM observation and cell attachment study. However, alkaline phosphatase activity was significantly increased on 3-D SF scaffold than on2-D nanofiber

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Effects of Calcium Phosphate Nanocrystals on Osseointegration of Titanium Implant in Irradiated Bone

BioMed Research International ◽

10.1155/2015/783894 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 4

Author(s):

Jun Yuan Li ◽

Edmond Ho Nang Pow ◽

Li Wu Zheng ◽

Li Ma ◽

Dora Lai Wan Kwong ◽

...

Keyword(s):

Growth Rate ◽

Calcium Phosphate ◽

Bone Growth ◽

Cell Attachment ◽

Titanium Implant ◽

Control Group ◽

Implant Surface ◽

Potential Alternative ◽

Etched Surface ◽

Surface Modified

Radiotherapy may compromise the integration of implant and cause implant loss. Implant surface modifications have the possibility of promoting cell attachment, cell growth, and bone formation which ultimately enhance the osseointegration process. The present study aimed to investigate the effects of calcium phosphate nanocrystals on implant osseointegration in irradiated bone. Sixteen rabbits were randomly assigned into control and nano-CaP groups, receiving implants with dual acid-etched surface or dual acid-etched surface discretely deposited of nanoscale calcium-phosphate crystals, respectively. The left leg of all the rabbits received 15 Gy radiation, followed by implants placement one week after. Four animals in each group were sacrificed after 4 and 12 weeks, respectively. Implant stability quotient (ISQ), ratio of bone volume to total volume (BV/TV), bone growth rate, and bone-to-implant contact (BIC) were evaluated. The nano-CaP group showed significantly higher ISQ (week 12,P=0.031) and bone growth rate (week 6,P=0.021; week 9,P=0.001) than that in control group. No significant differences in BV/TV and BIC were found between two groups. Titanium implant surface modified with CaP nanocrystals provides a potential alternative to improve bone healing around implant in irradiated bone.

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Impact of medical titanium implant surface on the efficiency of fibrointegration

Стоматология для всех ◽

10.35556/idr-2020-2(91)50-55 ◽

2020 ◽

pp. 50-55

Author(s):

T. R. Davydova ◽

А. I. Shaikhaliev ◽

D. A. Usatov ◽

G. A. Gasanov ◽

R. S. Korgoloev

Keyword(s):

Surface Roughness ◽

Titanium Dioxide ◽

Rough Surface ◽

Soft Tissues ◽

Titanium Implant ◽

Implant Surface ◽

Anatase Structure ◽

Bioactive Coating ◽

Cell Structures ◽

Titanium Alloy Ti6al4v

The aim of this study was to study the effect of surface branching of titanium endoprostheses on the efficiency of fibrointegration. The object of the study was samples of titanium alloy Ti6Al4V in the form of disks with a diameter of 5 mm and a thickness of 1 mm with various surface treatments: 1) samples with a rough surface after sandblasting; 2) samples with a rough surface after sandblasting with a bioactive coating of titanium dioxide TiO2 with anatase structure. The study of surface roughness was carried out by profilometry. Evaluation of the spreading and proliferation of cells on the surface of test samples, as well as evaluation of the effectiveness of fibrointegration was carried out according to standard methods using scanning electron microscopy. During the experiments, mesinchymal stem cells were sown on test samples and the test samples were introduced into the soft tissues of experimental animals. Based on the results obtained, it was concluded that the technology of forming rough surfaces by sandblasting does not provide high uniformity and reproducibility in the nanometer range and, apparently, another method for obtaining a rough surface should be chosen. The application of a bioactive coating of titanium dioxide TiO2 with the anatase structure to the surface of titanium endoprostheses increases the efficiency of fibrointegration, however, primarily the fibrointegration of titanium endoprostheses depends on their surface roughness, which determines the concentration of cell structures, the intensity of their adhesion and the ability to fibrointegrative process.

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Group III phospholipase A2 downregulation attenuated survival and metastasis in ovarian cancer and promotes chemo-sensitization

Journal of Experimental & Clinical Cancer Research ◽

10.1186/s13046-021-01985-9 ◽

2021 ◽

Vol 40 (1) ◽

Author(s):

Upasana Ray ◽

Debarshi Roy ◽

Ling Jin ◽

Prabhu Thirusangu ◽

Julie Staub ◽

...

Keyword(s):

Ovarian Cancer ◽

Cell Viability ◽

Phospholipase A2 ◽

Mtt Assay ◽

Metabolic Inhibitor ◽

Autophagic Flux ◽

Ciliated Cells ◽

Group Iii

Abstract Background Aberrant lipogenicity and deregulated autophagy are common in most advanced human cancer and therapeutic strategies to exploit these pathways are currently under consideration. Group III Phospholipase A2 (sPLA2-III/PLA2G3), an atypical secretory PLA2, is recognized as a regulator of lipid metabolism associated with oncogenesis. Though recent studies reveal that high PLA2G3 expression significantly correlates with poor prognosis in several cancers, however, role of PLA2G3 in ovarian cancer (OC) pathogenesis is still undetermined. Methods CRISPR-Cas9 and shRNA mediated knockout and knockdown of PLA2G3 in OC cells were used to evaluate lipid droplet (LD) biogenesis by confocal and Transmission electron microscopy analysis, and the cell viability and sensitization of the cells to platinum-mediated cytotoxicity by MTT assay. Regulation of primary ciliation by PLA2G3 downregulation both genetically and by metabolic inhibitor PFK-158 induced autophagy was assessed by immunofluorescence-based confocal analysis and immunoblot. Transient transfection with GFP-RFP-LC3B and confocal analysis was used to assess the autophagic flux in OC cells. PLA2G3 knockout OVCAR5 xenograft in combination with carboplatin on tumor growth and metastasis was assessed in vivo. Efficacy of PFK158 alone and with platinum drugs was determined in patient-derived primary ascites cultures expressing PLA2G3 by MTT assay and immunoblot analysis. Results Downregulation of PLA2G3 in OVCAR8 and 5 cells inhibited LD biogenesis, decreased growth and sensitized cells to platinum drug mediated cytotoxicity in vitro and in in vivo OVCAR5 xenograft. PLA2G3 knockdown in HeyA8MDR-resistant cells showed sensitivity to carboplatin treatment. We found that both PFK158 inhibitor-mediated and genetic downregulation of PLA2G3 resulted in increased number of percent ciliated cells and inhibited cancer progression. Mechanistically, we found that PFK158-induced autophagy targeted PLA2G3 to restore primary cilia in OC cells. Of clinical relevance, PFK158 also induces percent ciliated cells in human-derived primary ascites cells and reduces cell viability with sensitization to chemotherapy. Conclusions Taken together, our study for the first time emphasizes the role of PLA2G3 in regulating the OC metastasis. This study further suggests the therapeutic potential of targeting phospholipases and/or restoration of PC for future OC treatment and the critical role of PLA2G3 in regulating ciliary function by coordinating interface between lipogenesis and metastasis.

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Effect of Different Titanium Dental Implant Surfaces on Human Adipose Mesenchymal Stem Cell Behavior. An In Vitro Comparative Study

Applied Sciences ◽

10.3390/app11146353 ◽

2021 ◽

Vol 11 (14) ◽

pp. 6353

Author(s):

Vittoria D’Esposito ◽

Josè Camilla Sammartino ◽

Pietro Formisano ◽

Alessia Parascandolo ◽

Domenico Liguoro ◽

...

Keyword(s):

Cell Viability ◽

Dental Implant ◽

In Vivo Studies ◽

Implant Surface ◽

Cytokines And Chemokines ◽

Titanium Dental Implant ◽

Adhesive Ability ◽

Stimulating Factor

Background: The aim of this research was to evaluate the effects of three different titanium (Ti) implant surfaces on the viability and secretory functions of mesenchymal stem cells isolated from a Bichat fat pad (BFP-MSCs). Methods: Four different Ti disks were used as substrate: (I) D1: smooth Ti, as control; (II) D2: chemically etched, resembling the Kontact S surface; (III) D3: sandblasted, resembling the Kontact surface; (IV) D4: blasted/etched, resembling the Kontact N surface. BFP-MSCs were plated on Ti disks for 72 h. Cell viability, adhesion on disks and release of a panel of cytokines, chemokines and growth factor were evaluated. Results: BFP-MSCs plated in wells with Ti surface showed a viability rate (~90%) and proliferative rate comparable to cells plated without disks and to cells plated on D1 disks. D2 and D4 showed the highest adhesive ability. All the Ti surfaces did not interfere with the release of cytokines, chemokines and growth factors by BFP-MSCs. However, BFP-MSCs cultured on D4 surface released a significantly higher amount of Granulocyte Colony-Stimulating Factor (G-CSF) compared either to cells plated without disks and to cells plated on D1 and D2. Conclusions: The implant surfaces examined do not impair the BFP-MSCs cell viability and preserve their secretion of cytokines and chemokines. Further in vitro and in vivo studies are necessary to define the implant surface parameters able to assure the chemokines’ optimal release for a real improvement of dental implant osseointegration.

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Effect of Glucocorticoid-Induced Osteoporotic-Like Conditions on Osteoblast Cell Attachment to Implant Surface Microtopographies

Implant Dentistry ◽

10.1097/01.id.0000247858.37697.d5 ◽

2006 ◽

Vol 15 (4) ◽

pp. 377-385 ◽

Cited By ~ 4

Author(s):

Peter Cho ◽

Galen B. Schneider ◽

Bradley Kellogg ◽

Rebecca Zaharias ◽

John C. Keller

Keyword(s):

Cell Attachment ◽

Osteoblast Cell ◽

Implant Surface

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Biological characterization of implant surfaces - in vitro study

Revista de Odontologia da UNESP ◽

10.1590/1807-2577.1087 ◽

2015 ◽

Vol 44 (4) ◽

pp. 195-199 ◽

Cited By ~ 1

Author(s):

Priscilla Barbosa Ferreira Soares ◽

Camilla Christian Gomes Moura ◽

Huberth Alexandre da Rocha Júnior ◽

Paula Dechichi ◽

Darceny Zanetta-Barbosa

Keyword(s):

Alkaline Phosphatase ◽

Cell Viability ◽

Total Protein ◽

Cell Attachment ◽

Control Group ◽

Mitochondrial Activity ◽

Serum Total Protein ◽

Trypan Blue Exclusion ◽

Experimental Surface

<title>Abstract</title><sec><title>Objective</title>Evaluate the biological performance of titanium alloys grade IV under different surface treatments: sandblasting and double etching (Experimental surface 1; Exp1, NEODENT); surface with wettability increase (Experimental surface 2; Exp2, NEODENT) on response of preliminary differentiation and cell maturation.</sec><sec><title>Material and method</title>Immortalized osteoblast cells were plated on Exp1 and Exp2 titanium discs. The polystyrene plate surface without disc was used as control group (C). Cell viability was assessed by measuring mitochondrial activity (MTT) at 4 and 24 h (n = 5), cell attachment was performed using trypan blue exclusion within 4 hours (n = 5), serum total protein and alkaline phosphatase normalization was performed at 4, 7 and 14 days (n = 5). Data were analyzed using one-way ANOVA and Tukey test.</sec><sec><title>Result</title>The values of cell viability were: 4h: C– 0.32±0.01A; Exp1– 0.34±0.08A; Exp2– 0.29±0.03A. 24h: C– 0.43±0.02A; Exp1– 0.39±0.01A; Exp2– 0.37±0.03A. The cell adhesion counting was: C– 85±10A; Exp1- 35±5B; Exp2– 20±2B. The amounts of serum total protein were 4d: C– 40±2B; Exp1– 120±10A; Exp2– 130±20A. 7d: C– 38±2B; Exp1– 75±4A; Exp2– 70±6A. 14 d: C– 100±3A; Exp1– 130±5A; Exp2– 137±9A. The values of alkaline phosphatase normalization were: 4d: C– 2.0±0.1C; Exp1– 5.1±0.8B; Exp2– 9.8±2.0A. 7d: C– 1.0±0.01C; Exp1– 5.3±0.5A; Exp2– 3.0±0.3B. 14 d: C– 4.1±0.3A; Exp1– 4.4±0.8A; Exp2– 2.2±0.2B. Different letters related to statistical differences.</sec><sec><title>Conclusion</title>The surfaces tested exhibit different behavior at dosage of alkaline phosphatase normalization showing that the Exp2 is more associated with induction of cell differentiation process and that Exp1 is more related to the mineralization process.</sec>

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