scholarly journals Characterization of Bioactive compound isolated from Myrothecium spp. with UV, FTIR and HPLC Analysis

2015 ◽  
Vol 3 (01) ◽  
pp. 01-05 ◽  
Author(s):  
Mohammad Rizwan Pervez1 ◽  
Mohammed Musaddiq ◽  
Prashant V. Thakare ◽  
Ashutosh Kumar
Keyword(s):  
Ftir Spectroscopy ◽  
High Performance ◽  
Process Development ◽  
Opportunistic Infections ◽  
Antibiotic Production ◽  
Bioactive Compound ◽  
New Drugs ◽  
Hydroxyl Groups ◽  
Chromatography Analysis

Development of new drugs, especially in area of infectious diseases, represents today one of the most important research. Fungal isolates are receiving increasing attention by natural product chemists due to their diverse and structurally unprecedented compounds making them interesting candidates for drug discovery. In fact, need for novel, safe and more efficient antibiotics is a key challenge to the pharmaceutical industry today, moreover, increase in opportunistic infections in the immune compromised host has influenced this demand. Nowadays, evaluating morphological and biochemical differences as well as studying fungal genetic diversity via molecular indicators seem to be the most common method for screening this genus. In this research we evaluate the potential of bioactive compound, production and characterize the UV and FTIR spectroscopy and HPLC (High performance liquid chromatography) analysis pattern of isolated Myrothecium spp. MRP001. Process development for high level production of bioactive compound was applied using OFAT method. Then, following the extraction of secondary metabolite, the UV and FTIR spectroscopy analysis was carried out for characterization of the various extracts. Considering the coordinate analysis of UV and FTIR spectroscopy pattern, the isolate MRP001 with substantial antimicrobial activity exhibited absorption at 3411 cm-1 which is indicator of hydroxyl groups, absorption at 2856 and 2915 cm-1 indicating hydrocarbon chassis, and absorption at 1649 cm-1 indicating a double bond of polygenic compound. These results highlight the importance of Myrothecium isolates in antibiotic production. HPLC confirmed the production when compared with standards

scholarly journals Assessment of Quinone Enriched Fraction in Nigella sativa Seed Extracts

2020 ◽  
Vol 11 (2) ◽  
pp. 1505-1510
Author(s):  
Durga B ◽  
Durga B ◽  
Dass Prakash M V ◽  
Julius A
Keyword(s):  
High Performance ◽  
Nigella Sativa ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Bioactive Compound ◽  
New Drugs ◽  
Natural Sources ◽  
Ethanolic Extracts ◽  
Nigella Sativa Seed ◽  
Seed Extracts

In recent days, the thirst for the identification of the potential bioactive compounds from the natural sources like medicinal plants is on continuous demand. Among scientists and academicians, it has created many interdisciplinary platforms for research in establishing new drugs from the natural sources. According to many recent studies, Nigella sativa is believed to be the rich source of quinone, an effective bioactive compound with lots of medicinal values. The purpose of this study was to isolate and estimate the quinone in Nigella sativa seed extracts (aqueous and ethanol). Based on the qualitative and quantitative determination, the extracts were further focused for isolation of quinone from both aqueous and ethanolic  extracts of Nigella sativa. The isolated compound is identified by thin layer chromatography and purity is analyzed in High performance liquid chromatography. From the results we obtained, it was very clear that among the aqueous and ethanolic extracts of Nigella sativa, the ethanolic extract has been found with the highest quantity of quinine. This would be predicting that the ethanol extract of Nigella sativa may have good efficacy of pharmacological and therapeutic potentials like antidiabetic, anticancer, antimicrobial, anti-inflammatory properties when compared with the aqueous extract due to the presence of more quinone.

scholarly journals Characterization of Modified and Polymer Coated Alumina Surfaces by Infrared Spectroscopy

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Ashraf Yehia El-Naggar
Keyword(s):  
Infrared Spectroscopy ◽  
Alkali Metal ◽  
Ftir Spectroscopy ◽  
Surface Properties ◽  
Adsorption Properties ◽  
Hydroxyl Groups ◽  
Oh Groups ◽  
Structure Changes ◽  
The Individual

The prepared, modified, and coated alumina surfaces were characterized by infrared spectroscopy (FTIR) to investigate the surface properties of the individual and double modified samples. FTIR helps in reporting the changes occurred in hydroxyl groups as well as the structure changes as a result of thermal treating, hydrothermal treating, silylation treating, alkali metal treating, coating, and bonding with polymer. FTIR spectroscopy represents the strength and abundance of surface acidic OH which determine the adsorption properties of polar and nonpolar sorbents. Generally, all treated samples exhibit decrease of OH groups compared with those of parent ones producing alumina surfaces of different adsorptive powers.

scholarly journals Menaquinone (Vitamin K2) Biosynthesis: Localization and Characterization of the menA Gene fromEscherichia coli

1998 ◽  
Vol 180 (10) ◽  
pp. 2782-2787 ◽  
Author(s):  
K. Suvarna ◽  
D. Stevenson ◽  
R. Meganathan ◽  
M. E. S. Hudspeth
Keyword(s):  
High Performance ◽  
Promoter Sequence ◽  
Anaerobic Growth ◽  
Gene Encoding ◽  
Reading Frame ◽  
Chromatography Analysis ◽  
Naphthoic Acid ◽  
Membrane Bound ◽  
Carbon Side Chain

ABSTRACT A key reaction in the biosynthesis of menaquinone involves the conversion of the soluble bicyclic naphthalenoid compound 1,4-dihydroxy-2-naphthoic acid (DHNA) to the membrane-bound demethylmenaquinone. The enzyme catalyzing this reaction, DHNA-octaprenyltransferase, attaches a 40-carbon side chain to DHNA. The menA gene encoding this enzyme has been cloned and localized to a 2.0-kb region of the Escherichia coli genome between cytR and glpK. DNA sequence analysis of the cloned insert revealed a 308-codon open reading frame (ORF), which by deletion analyses was shown to restore anaerobic growth of amenA mutant. Reverse-phase high-performance liquid chromatography analysis of quinones extracted from theorf-complemented cells independently confirmed the restoration of menaquinone biosynthesis, and similarly, analyses of isolated cell membranes for DHNA octaprenyltransferase activity confirmed the introduction of the menA product into theorf-complemented menA mutant. The validity of an ORF-associated putative promoter sequence was confirmed by primer extension analyses.

Testing of Compositional Distribution in Brominated Isobutylene Elastomers

2004 ◽  
Vol 77 (1) ◽  
pp. 78-89
Author(s):  
F. Svec ◽  
J. M. J. Fréchet ◽  
I. Duvdevani
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Hplc Method ◽  
Normal Phase ◽  
Hydroxyl Groups ◽  
Bromine Content ◽  
Mobile Phases ◽  
Ethylene Dimethacrylate ◽  
Compositional Distribution

Abstract A method based on high-performance liquid chromatography (HPLC) method has been developed for the characterization of the compositional distribution of brominated poly(isobutylene-co-4-methylstyrene) and poly(isobutylene-co-isoprene). The desired separations in normal-phase chromatographic mode could only be achieved by using columns packed with specifically developed 10 and 3 µm porous monodisperse poly(2,3-dihydroxypropyl methacrylate-co-ethylene dimethacrylate) beads that have a homogeneous coverage of aliphatic hydroxyl groups. Linear gradients of tetrahydrofuran in hexane and dichloromethane in heptane were used respectively as the mobile phases for the selective elution of brominated poly(isobutylene-co-4-methylstyrenes) and brominated poly(isobutylene-co-isoprenes). In-column solvo-thermal treatment of the stationary phase further improved both selectivity and retention and enabled the easy detection and detailed quantification of differences in bromine content well below 1 mol %.

scholarly journals Glycosyltransferase FvCpsA Regulates Fumonisin Biosynthesis and Virulence in Fusarium verticillioides

Toxins ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 718
Author(s):  
Qi Deng ◽  
Hanxiang Wu ◽  
Qin Gu ◽  
Guangfei Tang ◽  
Wende Liu
Keyword(s):  
High Performance ◽  
Fusarium Verticillioides ◽  
Animal Health ◽  
Fumonisin B1 ◽  
Fungal Growth ◽  
Wall Stress ◽  
Ear Rot ◽  
Stalk Rot ◽  
Chromatography Analysis

Fusarium verticillioides is the major maize pathogen associated with ear rot and stalk rot worldwide. Fumonisin B1 (FB1) produced by F. verticillioides, poses a serious threat to human and animal health. However, our understanding of FB1 synthesis and virulence mechanism in this fungus is still very limited. Glycosylation catalyzed by glycosyltransferases (GTs) has been identified as contributing to fungal infection and secondary metabolism synthesis. In this study, a family 2 glycosyltransferase, FvCpsA, was identified and characterized in F. verticillioides. ΔFvcpsA exhibited significant defects in vegetative growth. Moreover, ΔFvcpsA also increased resistance to osmotic and cell wall stress agents. In addition, expression levels of FUM genes involved in FB1 production were greatly up-regulated in ΔFvcpsA. HPLC (high performance liquid chromatography) analysis revealed that ΔFvcpsA significantly increased FB1 production. Interestingly, we found that the deletion of FvCPSA showed penetration defects on cellophane membrane, and thus led to obvious defects in pathogenicity. Characterization of FvCpsA domain experiments showed that conserved DXD and QXXRW domains were vital for the biological functions of FvCpsA. Taken together, our results indicate that FvCpsA is critical for fungal growth, FB1 biosynthesis and virulence in F. verticillioides.

scholarly journals CHARACTERIZATION OF THERMOSTABLE BETA-1,4-GALACTANASE AND ITS APPLICATION IN HYDROLYSIS OF PECTIN FROM SWEET POTATO (Ipomoea batatas (L.) Lam) PEELS

2021 ◽  
Vol 83 (5) ◽  
pp. 57-65
Author(s):  
Noor Faizah Ismail ◽  
Dayang Norulfairuz Abang Zaidel ◽  
Mohd Noor Mat Isa
Keyword(s):  
Sweet Potato ◽  
High Performance ◽  
Ipomoea Batatas ◽  
Catalytic Efficiency ◽  
Tween 20 ◽  
E Coli ◽  
Chromatography Analysis ◽  
Potato Peels ◽  
Hydrolysis Of

Galactooligosaccharides (GOS) synthesis has received much attention due to its prebiotic function. Beta-1,4-galactanase responsible for the hydrolysis of galactan plays an important role in producing GOS from biodegradation of this pectin component. In this study, beta-1,4-galactanase (BgcGC) from a thermophilic Geobacillus mahadii Geo-05 was heterologously expressed in Escherichia coli (E. coli) and characterized. The optimum temperature of BgcGC was at 60°C and stable from 20-60°C while optimum pH was at 6 and stable from pH 4-10. BgcGC showed high catalytic efficiency towards potato galactan (873.8 ml mg-1 s-1) and lupin galactan (1694.4 ml mg-1 s-1). The activity of BgcGC was not significantly affected with the presence of 100 mM K+, Tween-20 and 2-mercaptoethanol. Application of BgcGC towards pectin-containing galactan oligomer extracted from sweet potato peels resulted in galactose and GOS synthesis as revealed by high performance liquid chromatography analysis. Thus, this enzyme has a potential to be one of the enzyme candidates involves in pectin complex degradation to produce GOS.

scholarly journals Characterization of a Novel N-Acylhomoserine Lactonase RmmL from Ruegeria mobilis YJ3

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 370 ◽  
Author(s):  
Xiulei Cai ◽  
Min Yu ◽  
Hu Shan ◽  
Xiaorong Tian ◽  
Yanfen Zheng ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
High Performance ◽  
Vibrio Anguillarum ◽  
Cell Communication ◽  
Quorum Quenching ◽  
Amino Acid Sequence Analysis ◽  
Chromatography Analysis ◽  
Potential Strategy

Gram-negative bacteria utilize N-acylhomoserine lactones (AHLs) as quorum sensing (QS) signaling molecules for intercellular communication. Cell-to-cell communication depends on cell population density, and AHL-dependent QS is related to the production of multiple genes including virulence factors. Quorum quenching (QQ), signal inactivation by enzymatic degradation, is a potential strategy for attenuating QS regulated bacterial infections. Both Gram-positive and -negative bacteria have QQ enzymes that can degrade AHLs. In our previous study, strain Ruegeria mobilis YJ3, isolated from healthy shrimp, showed strong AHLs degradative activity. In the current study, an AHL lactonase (designated RmmL) was cloned and characterized from Ruegeria mobilis YJ3. Amino acid sequence analysis showed that RmmL has a conserved “HXHXDH” motif and clusters together with lactonase AidC that belongs to the metallo-β-lactamase superfamily. Recombinant RmmL could degrade either short- or long-chain AHLs in vitro. High-performance liquid chromatography analysis indicated that RmmL works as an AHL lactonase catalyzing AHL ring-opening by hydrolyzing lactones. Furthermore, RmmL can reduce the production of pyocyanin by Pseudomonas aeruginosa PAO1, while for the violacein and the extracellular protease activities by Chromobacterium violaceum CV026 and Vibrio anguillarum VIB72, no significant reduction was observed. This study suggests that RmmL might be used as a therapeutic agent in aquaculture.

scholarly journals Isolation from Agricultural Soil and Characterization of a Sphingomonas sp. Able To Mineralize the Phenylurea Herbicide Isoproturon

2001 ◽  
Vol 67 (12) ◽  
pp. 5403-5409 ◽  
Author(s):  
Sebastian R. Sørensen ◽  
Zeev Ronen ◽  
Jens Aamand
Keyword(s):  
High Performance ◽  
Agricultural Soil ◽  
Side Chain ◽  
Rrna Gene ◽  
Chromatography Analysis ◽  
Phenylurea Herbicide ◽  
Partial Analysis ◽  
Pure Bacterial Culture ◽  
The 16S Rrna Gene

ABSTRACT A soil bacterium (designated strain SRS2) able to metabolize the phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1,1-dimethylurea (IPU), was isolated from a previously IPU-treated agricultural soil. Based on a partial analysis of the 16S rRNA gene and the cellular fatty acids, the strain was identified as a Sphingomonas sp. within the α-subdivision of the proteobacteria. Strain SRS2 was able to mineralize IPU when provided as a source of carbon, nitrogen, and energy. Supplementing the medium with a mixture of amino acids considerably enhanced IPU mineralization. Mineralization of IPU was accompanied by transient accumulation of the metabolites 3-(4-isopropylphenyl)-1-methylurea, 3-(4-isopropylphenyl)-urea, and 4-isopropyl-aniline identified by high-performance liquid chromatography analysis, thus indicating a metabolic pathway initiated by two successive N-demethylations, followed by cleavage of the urea side chain and finally by mineralization of the phenyl structure. Strain SRS2 also transformed the dimethylurea-substituted herbicides diuron and chlorotoluron, giving rise to as-yet-unidentified products. In addition, no degradation of the methoxy-methylurea-substituted herbicide linuron was observed. This report is the first characterization of a pure bacterial culture able to mineralize IPU.

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