Mutant P53 Expression Of Oral Transformed Epithelium Cell In Rats Injected By Benzo[A]Pyrene

Anaerobic exercise is a high-intensity exercise that needs quick energy supplies obtained in a very short time. However, this exercise may result in tissue hypoxia which is characterized by the increase of HIF-1α concentration. The presence of HIF-1α will induce the secretion of VEGF and, eventually, trigger angiogenesis. Nevertheless, it is still unclear whether anaerobic exercise will also cause hypoxia in which this condition will increase the concentration of VEGF in brain tissues. The aim of this study was to find out the effect of anaerobic exercise frequency towards VEGF concentration of Wistar rat brain tissues. Brain tissues were taken from rats treated with anaerobic exercise using treadmill. This exercise was given in different frequencies; one time, three times, and seven times a week. The data collected were analyzed using independent t-test. The results of this study showed that anaerobic exercise done once a week could significantly increase VEGF concentration (p < 0.05) if compared with the one in control group (95.21 ± 31.99 v.s. 63.36 ± 11.01 pg/mL). Meanwhile, VEGF concentration of treatment groups given exercise three times a week (47.97 ± 10.68 pg/mL) and seven times a week (40.56 ± 13.98 pg/mL) showed a significant decrease if compared with that of control group (63.36 ± 11.01 pg/mL). Anaerobic exercise affected VEGF concentration as an indicator of angiogenesis in brain tissue of wistar rats.

Download Full-text

Effects on Gingivitis of Two Different 0.4% SnF2 Gels

Journal of Dental Research ◽

10.1177/00220345880670021501 ◽

1988 ◽

Vol 67 (2) ◽

pp. 503-507 ◽

Cited By ~ 21

Author(s):

R.L. Boyd ◽

P.J. Leggott ◽

P.B. Robertson

Keyword(s):

Treatment Group ◽

High Availability ◽

Plaque Index ◽

Control Group ◽

Bleeding Tendency ◽

Gingival Index ◽

Periodontal Health ◽

Pocket Depth ◽

Treatment Groups ◽

The One

For nine months we monitored the periodontal health of 81 adolescents undergoing orthodontic treatment with fixed appliances, to determine whether daily use of a brush-on 0.4% SnF 2 gel would be better than toothbrushing alone in maintaining periodontal health in these patients, and whether a gel supplying a high percentage of available Sn2+ ion would be more beneficial than a gel supplying a low percentage of available Sn2+ ion. The subjects were matched for age and sex and placed into a control group, which used toothbrushing alone, and two treatment groups, which used toothbrushing supplemented with daily use of a SnF2 gel. One treatment group used a gel with 98% available Sn2+ ion twice daily for the entire nine months. The other treatment group used a gel with less than 2% available Sn2+ once a day for six months, then twice a day for the remaining three months of the study. Clinical assessments (Plaque Index, Gingival Index, Bleeding Tendency, pocket depth, and coronal staining) were made before appliances were placed and at one, three, six, and nine months after appliances were placed. Results indicated that the group using the high-availability Sn 2+ gel twice daily had significantly lower Gingival Index and Bleeding Tendency scores at the one-, three-, six-, and nine-month examinations than did the control group. The group using the low-availability Sn2+ gel showed no significant differences in these assessments from the control group. Neither treatment group showed significant differences from the control group in Plaque Index or pocket depth. In the group using the high-availability Sn2+ gel, one subject developed mild coronal staining, and two developed moderate staining.

Download Full-text

The effect of exposure to liquid air freshener on histological of the bronchial mice (Mus musculus)

Edubiotik Jurnal Pendidikan Biologi dan Terapan ◽

10.33503/ebio.v6i02.1219 ◽

2021 ◽

Vol 6 (02) ◽

Author(s):

Warobi Warobi ◽

Delima Engga Maretha ◽

Asnilawati Asnilawati ◽

Mashuri Masri

Keyword(s):

Mus Musculus ◽

The Body ◽

Control Group ◽

Epithelial Tissue ◽

Treatment Groups ◽

Histological Picture ◽

Randomized Design ◽

Post Test ◽

The One ◽

Post Hoc

Air fresheners are products that contain chemicals aimed at reducing unpleasant odours in confined spaces. The use of synthetic air fresheners turns out to harm health because some of their leased Volatile Organic Compounds are classified as toxic compounds and are carcinogens. Air freshener enters the body through the inhalation process in the respiratory system. Modern air fresheners are available in liquid (aerosol) and gel forms. Air fresheners contain addictive substances and solvents such as 1,4-dichlorobenzene which can affect pulmonary function. The purpose of this study is to know the effect of exposure to liquid air freshener on the histology of bronchi of mice (Mus musculus). This study used 20 male mice (Mus musculus) consisting of 4 treatments with 5 replications. The research design is true experimental in the form of a post-test only control group with Completely Randomized Design (CRD). The post-test was done by observing the histological picture of the mice's bronchi after exposure to liquid air freshener given 3x/day for a period of P1 = 2 weeks, P2 = 4 weeks and P3 = 6 weeks. Quantitative data on bronchial histology was tested using the One Way ANOVA statistical test followed by the Post Hoc Tukey test. The results of the study found changes in the histology of the bronchi, thickening of the epithelial tissue of mice. Analysis of comparative data between the control and treatment groups statistically obtained p = 0.010 <0.05, meaning that there was a significant effect and change in the bronchial tubes exposed to liquid air freshener. Histologically there were differences in histology between the control and treatment groups. It was concluded that liquid air freshener had a significant effect on the histological picture of the mice's bronchi.

Download Full-text

ADDITION OF WHITE SHRIMP (Penaeus merguiensis) HEAD EXTRACT TO INCREASE PROTEIN CONTENT IN SHRIMP STICKS

TROPICAL WETLAND JOURNAL ◽

10.20527/twj.v2i1.20 ◽

2016 ◽

Vol 2 (1) ◽

pp. 15-21

Author(s):

Rabiatul Adawyah

Keyword(s):

Treatment Group ◽

Protein Content ◽

White Shrimp ◽

Control Group ◽

Treatment Groups ◽

Randomized Design ◽

Increase Protein Content ◽

Shrimp Head ◽

The One ◽

Shrimp Heads

The objective of this study was to obtain a proper ratio between the volume of water and the weight of shrimp heads in order to increase the protein content in shrimp sticks. The design used in the study was a completely randomized design (CRD) with treatment groups of 1:1, 1:2, and a control group without the addition of shrimp head extract. Water content, protein content, ash content and organoleptic test were the parameters measured. The results of the study show that the addition of white shrimp head extract in the treatment group with a 1: 1 ratio produced shrimp sticks with a protein content of 12.22%; in the treatment group with a 1: 2 ratio 13.44%; and in the control group without the addition of shrimp head extract only 5.54%. The addition of shrimp head extract also produced more preferred flavor compared to the one without the addition of the extract.

Download Full-text

Effects of liquid ionic silver concentration on caspase-3 and p53mt expressions in the oral mucosal epithelium of Wistar rats

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v51.i3.p138-142 ◽

2018 ◽

Vol 51 (3) ◽

pp. 138

Author(s):

R. Aries Muharram ◽

I. Istiati ◽

Pratiwi Soesilawati

Keyword(s):

Wistar Rats ◽

Caspase 3 ◽

Silver Concentration ◽

Control Group ◽

Oral Epithelium ◽

Mutant P53 ◽

Research Subjects ◽

P53 Expression ◽

Mucosal Epithelium ◽

Ionic Silver

Background: Silver, especially oxidized silver, has been used as a medicine considered to have bactericidal properties. In the present day, ionic silver (Ag+) is also used in the manufacture of cosmetics, socks, food containers, detergents, sprays and other products to prevent the spread of germs. Unfortunately, ionic silver is assumed to be toxic not only to bacteria, but also to humans and the environment. Therefore, it is essential to identify the optimum dosage of ionic silver considered safe by investigating the effects of ionic silver concentration on cell death through activation of mutant p-53 expression by caspase 3 in the oral epithelium. Purpose: This research aimed to analyze the effects of concentrated liquid ionic silver (Ag+) on caspase-3 and mutant p53 expression in the oral mucosal epithelium. Methods: This research constituted a laboratory-based experimental study with posttest-only design. The research subjects consisted of 28 Wistar rats, divided into four treatment groups, namely; KK (with Aquadest), KP 1 (with 5% liquid ionic silver), KP 2 (with 10% liquid ionic silver) and KP 3 (with 15% liquid ionic silver). Each rat was then treated orally with 0.5ml of liquid ionic silver at fixed concentrations twice a day for seven days. The Wistar rats were then terminated and their tissue samples processed by means of histopathological and immunohistochemical staining examination. The monoclonal Caspase-3 and mutant p53 expressions in each group were evaluated with the data being tabulated and analyzed statistically. Results: Mutant p53 expression was also found in the control group. Moreover, the higher the concentration of liquid ionic silver, the greater the elevated Caspase-3 and mutant p53 expressions. Conclusion: The concentration of liquid ionic silver plays an important role in elevating Caspase-3 and mutant p53 expressions.

Download Full-text

Could information about herd immunity help us achieve herd immunity? Evidence from a population representative survey experiment

Scandinavian Journal of Public Health ◽

10.1177/1403494818770298 ◽

2018 ◽

Vol 46 (8) ◽

pp. 854-858 ◽

Cited By ~ 5

Author(s):

Sveinung Arnesen ◽

Kristine Bærøe ◽

Cornelius Cappelen ◽

Benedicte Carlsen

Keyword(s):

Survey Design ◽

Herd Immunity ◽

Control Group ◽

Health Campaigns ◽

Treatment Groups ◽

Hypothetical Scenario ◽

Concern For Others ◽

Public Health Campaigns ◽

The One ◽

The Individual

Aims: Immunisation causes dramatic reductions in morbidity and mortality from infectious diseases; however, resistance to vaccination is nonetheless widespread. An understudied issue – explored here – is whether appeals to collective as opposed to individual benefits of vaccination encourage people to vaccinate. Knowledge of this is important not least with respect to the design of public health campaigns, which often lack information about the collective benefits of vaccination. Methods: Using a between-subjects experimental survey design, we test whether information about the effects of herd immunity influences people’s decision to vaccinate. A representative sample of Norwegians was confronted with a hypothetical scenario in which a new and infectious disease is on its way to Norway. The sample was split in three – a control group and two treatment groups. The one treatment group was provided information about collective benefits of vaccination; the other was provided information about the individual benefits of vaccination. Results: Both treatments positively affect people’s decision to vaccinate; however, informing about the collective benefits has an even stronger effect than informing about the individual benefits. Conclusions: Our results suggest that people’s decision about whether to vaccinate and thus contribute to herd immunity is influenced by concern for others. Thus, stressing the collective benefits of vaccination could increase the effectiveness of health campaigns.

Download Full-text

Role of Superoxide Dismutases (SODs) in Hypertensive Rats After Administration of Mango Mistletoe Methanolic Extract

JSMARTech ◽

10.21776/ub.jsmartech.2021.002.02.61 ◽

2021 ◽

Vol 2 (2) ◽

pp. 061-064

Author(s):

Mariyam Suroyya ◽

◽

Nour Athiroh Sjakoer ◽

Nurul Mubarakati ◽

◽

...

Keyword(s):

Methanolic Extract ◽

White Male ◽

Male Rats ◽

Control Group ◽

Hypertensive Rats ◽

Treatment Groups ◽

Group A ◽

The One ◽

Endogenous Antioxidant ◽

Post Hoc

Hypertension is a condition where there is an abnormal rise in blood pressure that may be the primary cause of cardiovascular disease. Hypertension induces the production of free radicals known as reactive oxygen species (ROS) and oxidative stress. The purpose of this study is to further examine the function of Dendropthoe pentandra as an endogenous antioxidant modulator in this case superoxide dismutase (SOD) in hypertensive rats.The testing approach used is experimental. Data were analyzed using the one-way ANOVA test and the Post Hoc test to see variations in SOD levels in different treatments. This research used a hypertensive rat model induced by deoxycorticosterone acetate (DOCA) and salt. The number of animals tested was 25 white male rats divided into 5 groups, each containing 5 rats.The group consisted of a control group, a group of non- Extract methanolic of mango mistletoe hypertensive rats, and three groups of hypertensive rats receiving mango mistletoe methanolic extract (EMBM) at dosages of 50 mg/kg, 100 mg/kg, and 200 mg/kg body weight. Based on the results of the study, it is known that the levels of increased lung SOD with extract methanolic of mango mistletoe dosage variations in all treatment groups were not different. The administration of mango parasite methanolic extract at a dose of 50 mg / kgBW was optimum in increasing lung SOD levels in hypertensive rats.

Download Full-text

Vascular Endothelial Growth Factor Concentration in Brain of Rat Treated with Anaerobic Exercises

International Journal of Public Health Science (IJPHS) ◽

10.11591/.v4i3.4734 ◽

2015 ◽

Vol 4 (3) ◽

pp. 201

Author(s):

Rostika Flora ◽

Muhammad Zulkarnain ◽

Yuliana Ardi ◽

Esti Sorena ◽

Roslina Wati ◽

...

Keyword(s):

Wistar Rat ◽

Anaerobic Exercise ◽

Control Group ◽

Vascular Endothelial ◽

Exercise Frequency ◽

Treatment Groups ◽

The One ◽

Short Time ◽

Endothelial Growth Factor ◽

Brain Tissues

Anaerobic exercise is a high-intensity exercise that needs quick energy supplies obtained in a very short time. However, this exercise may result in tissue hypoxia which is characterized by the increase of HIF-1α concentration. The presence of HIF-1α will induce the secretion of VEGF and, eventually, trigger angiogenesis. Nevertheless, it is still unclear whether anaerobic exercise will also cause hypoxia in which this condition will increase the concentration of VEGF in brain tissues. The aim of this study was to find out the effect of anaerobic exercise frequency towards VEGF concentration of Wistar rat brain tissues. Brain tissues were taken from rats treated with anaerobic exercise using treadmill. This exercise was given in different frequencies; one time, three times, and seven times a week. The data collected were analyzed using independent t-test. The results of this study showed that anaerobic exercise done once a week could significantly increase VEGF concentration (p < 0.05) if compared with the one in control group (95.21 ± 31.99 v.s. 63.36 ± 11.01 pg/mL). Meanwhile, VEGF concentration of treatment groups given exercise three times a week (47.97 ± 10.68 pg/mL) and seven times a week (40.56 ± 13.98 pg/mL) showed a significant decrease if compared with that of control group (63.36 ± 11.01 pg/mL). Anaerobic exercise affected VEGF concentration as an indicator of angiogenesis in brain tissue of wistar rats.

Download Full-text

Pengaruh Perendaman Minuman Berkarbonasi terhadap Gaya Elastik pada Elastomerik Ligatur dengan Variasi Panjang Penarikan

e-GIGI ◽

10.35790/eg.v9i2.34554 ◽

2021 ◽

Vol 9 (2) ◽

pp. 282

Author(s):

Hafidz I. Pradipta ◽

Budi Wibowo ◽

Diah A, Purbaningrum ◽

Yoghi B. Prabowo

Keyword(s):

Testing Machine ◽

Elastic Force ◽

Control Group ◽

Control Group Design ◽

Treatment Groups ◽

Post Test ◽

Carbonated Drinks ◽

The One ◽

Post Hoc ◽

One Way Anova

Abstract: Elastomeric ligature is used to fasten orthodontic wires with brackets and can be stretched 1-3 mm as a simulation of bracket in the oral cavity. Its elastic force could decrease caused by salivary pH and water absorption of the elastomeric ligature. Consuming carbonated drinks causes the oral cavity to become acidic which increases the loss of elastic force in the elastomeric ligature with length of stretch variations. This study was aimed to determine the effect of immersion in carbonated drinks on the elastic force of the elastomeric ligature with length of stretch variations. This was an experimental laboratory study with a post-test only control group design. There were a total of 24 samples of American Orthodontic brand elastomeric ligature with royal blue color divided into six groups, consisting of three control groups immersed in artificial saliva, and three treatment groups immersed in carbonated drinks. Each control group and treatment group were given three variations of stretch length, as follows: 1, 2, and 3 mm. The calculation of the elastic force on the elastomeric ligature was performed by using the Universal Testing Machine. Data were analyzed by using the One Way Anova test and LSD post hoc test (p <0.05). The One Way Anova test showed a significant difference in the 1, 2, and 3 mm length of stretch variations for treatment groups. In conclusion, carbonated drinks affect the elastic force of elastomeric ligature with length of stretch variations.Keywords: elastomeric ligature; carbonated drinks; length of stretch variations Abstrak: Elastomerik ligatur adalah alat yang digunakan untuk mengikatkan kawat ortodonti dengan braket, dapat diregangkan 1-3 mm sebagai simulasi braket dalam rongga mulut. Elastomerik ligatur mengalami penurunan gaya elastik salah satunya disebabkan oleh pH saliva, dan penyerapan air dari elastomerik ligatur. Mengonsumsi minuman berkarbonasi menyebabkan pH saliva menjadi asam sehingga meningkatkan kehilangan gaya elastik pada elastomerik ligatur dengan variasi panjang penarikan. Tujuan penelitian ini untuk mengetahui pengaruh perendaman minuman berkarbonasi terhadap gaya elastik pada elastomerik ligatur dengan variasi panjang penarikan. Desain penelitian ialah eksperimental laboratorium dengan post-test only control group design. Sebanyak 24 sampel elastomerik ligatur merek American Orthodontic dengan warna royal blue, dibagi menjadi enam kelompok, terdiri dari tiga kelompok kontrol perendaman pada saliva buatan, dan tiga kelompok perlakuan perendaman pada minuman berkarbonasi. Setiap kelompok kontrol dan perlakuan diberi tiga variasi panjang penarikan, yaitu penarikan 1, 2, dan 3 mm. Perhitungan gaya elastik pada elastomerik ligatur menggunakan alat Universal Testing Machine. Analisis data menggunakan uji One Way Anova dan uji post hoc LSD pada p<0,05. Hasil uji One Way Anova menunjukkan perbedaan bermakna pada penarikan 1, 2, 3 mm dalam perendaman minuman berkarbonasi. Simpulan penelitian ini ialah terdapat pengaruh perendaman minuman berkarbonasi terhadap gaya elastik pada elastomerik ligatur dengan variasi panjang penarikan.Kata kunci: elastomerik ligatur; minuman berkarbonasi; variasi panjang penarikan

Download Full-text

Black Cumin Seed Oil Antidiabetogenic by Increasing Pancreatic P53 Expression

International Journal of Public Health Science (IJPHS) ◽

10.11591/ijphs.v7i3.13694 ◽

2018 ◽

Vol 7 (3) ◽

pp. 207

Author(s):

Akrom Akrom ◽

Rizma Nurfadjrin ◽

Endang Darmawan ◽

Titiek Hidayati

Keyword(s):

Seed Oil ◽

Positive Control ◽

Control Group ◽

Sprague Dawley ◽

P53 Expression ◽

Black Cumin ◽

Treatment Groups ◽

Glucose Levels ◽

Black Cumin Seed ◽

Cumin Seed

Black cumin seed oil (BCSO) contains many thymoquinone and unsaturated fatty acids that act as antioxidants. Thymoquinone was thought to inhibit pancreatic damage and prevent hyperglycemia by involving p53. The study aimed to determine the BCSO effect on Malondialdehyde (MDA) levels and p53 expression of pancreatic tissues in alloxan-induced Sprague Dawley (SD) rats. This study used Pre and Post Test with Control Group Design with 49 male Sprague Dawley rats aged 30 days weighing 150 - 300 gram. Rats were divided into 7 groups: Group I, the normal control group receiving standard feeding and drinking; Group II, the negative control group receiving alloxan; Group III & IV, the treatment groups, receiving BCSO with a dose of 6.9 mg/KgBW (BCSO6.8) and 68 mg/KgBW (BCSO68 group); Group V, the positive control 1 group receiving simvastatin 10 mg/KgBW; Group VI, the positive control 2 group receiving vitamin C 18 mg/200mgBW; Group VII, the solvent control receiving DMSO. The treatment groups received two different doses per day for 9 days orally. Blood MDA and glucose levels were determined by spectrophotometry and p53 expression of pancreas tissues were read by a pathologist. The results show that the BCSO68 group had a decreased activity of MDA and glucose but a higher expression of p53 in pancreatic tissues compared to the BCSO6.8 group. Decreased Malondialdehyde levels in the BCSO68 group were similar to those in the vitamin C group but lower than those in the simvastatin group (p<0.05). It can be concluded that the BCSO administration of 68 mg/KgBW per day can decrease blood Malondialdehyde and glucose levels and increase p53 expression.

Download Full-text