Analisis Kandungan Mikroba, Formalin, dan Timbal (Pb) pada Tahu Sumedang yang Dijual Di Daerah Macet Cicurug, Ciawi, dan Cisarua Jawa Barat

2020 ◽  
Vol 6 (1) ◽  
pp. 087
Author(s):  
Rosy Hutami ◽  
M Fakih Kurniawan ◽  
Henna Khoerunnisa
Keyword(s):  
Content Analysis ◽  
Microbial Contamination ◽  
Atomic Absorption Spectrophotometry ◽  
Plate Count ◽  
Traffic Jam ◽  
Absorption Spectrophotometry ◽  
Traffic Jams ◽  
Total Plate Count ◽  
Microbial Analysis ◽  
Aas Method

Sumedang tofu is one of favorite foods for Indonesian society. But many sellers or producers are not aware to the food safety of sumedang tofu. The aims of this study were to analyze the microbial, formalin, and lead (Pb) contents in ready-to-eat sumedang tofu which were sold in traffic jams area in Cicurug, Ciawi, and Cisarua. The analysis were carried out by Total Plate Count (TPC) testing for microbial analysis, potassium permanganate reaction (KMnO4) testing for formaldehyde analysis, and atomic absorption spectrophotometry (AAS) method for lead content analysis in the samples. The results obtained for the microbial analysis were sumedang tofu that were sold in the traffic jam areas of Cicurug, Ciawi, and Cisarua contained contaminant above the treshold (1.4 x 105 colonies / gram to 2.2 x 105 colonies / gram of microbes). All of the samples of sumedang tofu were positive containing formaldehyde. Otherwise, there were no lead (Pb) content in all samples regarding to AAS analysis. This study concluded that the ready-to-eat sumedang tofu those were sold in traffic jam area in the Cicurug, Ciawi, and Cisarua were not suitable for consumption because it contained exceed microbial contamination and formalin which are harmful for human health.Keywords : formalin, microbes, sumedang tofu, lead, traffic jam

scholarly journals Reduction of Microbe Contamination through Steaming Process to Cocoa Beans Using Steaming Chamber

2014 ◽  
Vol 30 (1) ◽  
Author(s):  
Hendy Firmanto
Keyword(s):  
Microbial Contamination ◽  
Microbial Population ◽  
Plate Count ◽  
Cocoa Bean ◽  
Contamination Level ◽  
Cocoa Beans ◽  
Total Plate Count ◽  
Microbial Analysis ◽  
Plate Count Method ◽  
Nutrient Changes

Dry cocoa bean quality is also determined by its microbe contamination level. Steaming process for dried cocoa beans as a pretreatment process was selected because of less effect on organic compound inside the dried cocoa bean. This experiment aim was to study microbial contamination level of cocoa beans using steaming process, determining its microbial population and evaluate its chemical changes. Experiment was carried out in Postharvest Laboratory of Indonesian Coffee and Cocoa Research Institute. Cocoa beans for the experiment were lots collected from four farms in Jayapura, Papua with different microbial contamination level for each lot. Results of this experiment showed that optimum steaming process was 15 minutes at 100 O C with 10 minutes preheating time. Microbial analysis result of the four lots after complete steaming process by total plate count method showed the same result (<3.0 x 103 cfu). Most of the decrease in microbial contaminant appeared in the plate was 73.5% of Staphylococcus aureusand 0.058% of Penicilliumsp. Bean acidity (pH) after steaming increased (4.76 to 4.80) and free fatty acid increased (1.81% to 1.96%) while carbohydrate content decreased (17.5% to 15.9%) and as well as protein content (12.6% into 11.7%). Key words: cocoa bean, steaming process, microbe reduction, nutrient changes

scholarly journals Uji Total Plate Count (TPC) Bakteri Pada Minuman Teh Poci Homemade di Gampong Batoh Banda Aceh

2021 ◽  
Vol 6 (2) ◽  
Author(s):  
Yuni Dewi Safrida ◽  
Hardiana Hardiana ◽  
Mauliyana Mauliyana
Keyword(s):  
Random Sampling ◽  
Microbial Contamination ◽  
Plate Count ◽  
Total Plate Count ◽  
Hygienic Quality ◽  
Experimental Laboratory ◽  
Banda Aceh

Poci tea is a favorite beverage produced by household industry. Considering its hygienic quality so that it is not contaminated by microbes seems to be an important factor. This study aims to determine the level of microbial contamination in Poci tea produced by household retailers in Batoh, Banda Aceh. This study was conducted at the Pharmacy Laboratory, Academy of Pharmacy and Food Analysts (AKAFARMA) Banda Aceh from 8  to 12 June 2020. The method of this study is    a quantitative experimental laboratory with Total Plate Count (TPC). The samples of this study are seven Poci tea  obtained from Batoh retailers selected by random sampling. The results showed that the seven Poci teas samples were contaminated  microbes, i.e ALMK with a TPC value of 4x103 colonies / ml, AMK with a TPC value of 4x103 colonies / ml, HFK with a TPC value of 2x103 colonies / ml, JLM with a TPC value of 3x103 colonies / ml, KUCB with a TPC value of 4x103 colonies / ml, MHFK with a TPC value of 4x103 colonies / ml, and MHZZ with a TPC value of 2x103 colonies / ml. In conclusion, the seven Poci tea samples sold in Batoh have TPC values that are not permitted by the Regulation of the Head of RI POM No. 16 of 2016. Therefore, this poci tea is not recommended for consumption.

scholarly journals Physico-sensory and Textural Properties of Composite Millet Palm Jaggery Muffins

2021 ◽  
Vol 21 (1) ◽  
pp. 37
Author(s):  
Snehal Jadhav ◽  
V. Kavinya ◽  
R. Vijay Nirmal ◽  
H. Mohammed Shameem ◽  
K. Ramalakshmi
Keyword(s):  
Textural Properties ◽  
Cane Sugar ◽  
Quality Parameters ◽  
Plate Count ◽  
Total Plate Count ◽  
Brown Colour ◽  
Sensory Score ◽  
Microbial Analysis ◽  
And Control ◽  
Mold Count

Composite millet palm jaggery (CMPJ) muffins were prepared by replacing all-purpose flour with composite millet flour at different level (0:100, 50:50, 30:70) and replacing cane sugar completely with palm jaggery. CMPJ muffins and control muffins with cane sugar (APFS) were analyzed for Physico-sensory and textural properties (TPA). Incorporation of millet flour and wheat flour at the ratio 70:30 resulted in an acceptable product with good sponginess which is one of the desirable properties of muffins. The moisture content of muffins prepared with palm jaggery was found to be higher (21.84±0.01%) than muffins prepared with sugar (19.58 ±0.01%). The lightness of the crumb and crust (37.58±0.08, 28.89±0.11) of CMPJ muffins were found to be lesser than control muffins (58.34±0.20, 35.30±0.99), whereas redness of crumb and crust was found more in CMPJ muffins (9.18±0.07, 12.12±0.22) than APFS muffins (6.56±0.15, 10.61 ±0.15) which is due to the brown colour of palm jaggery. The muffins with jaggery had lower pH and sensory score and higher water activity (aw) than muffins with sugar. TPA results showed that CMPJ (6270±7.2 g) were slightly harder than APFS (4729±4.7). Microbial analysis (Total plate count, Yeast and mold count) for CMPJ muffins was found to be safe for consumption upto 12 days without added preservative. It is concluded that CMPJ muffins (without preservative) can be an alternative to APFS without affecting the quality parameters of the product.

Atomic Absorption Spectrophotometric Method for Determination of Water-Soluble Copper in Water-Insoluble Copper Fungicides: CIPAC Collaborative Study. Comparison with Bathocuproine Method

1981 ◽  
Vol 64 (1) ◽  
pp. 75-78
Author(s):  
F Sánchez Rasero ◽  
◽  
P G Balayannis ◽  
H P Beyers ◽  
E Celma ◽  
...  
Keyword(s):  
Atomic Absorption ◽  
Collaborative Study ◽  
The United States ◽  
Atomic Absorption Spectrophotometry ◽  
Water Soluble ◽  
Absorption Spectrophotometry ◽  
Aas Method ◽  
Standard Deviations ◽  
The Difference

Abstract An atomic absorption spectrophotometric (AAS) method was collaboratively studied by 8 laboratories from Africa, the United States, Australia, and Europe. The samples were dispersed in deionized water. After centrifuging and filtering, the water-soluble copper in the filtrate was acidified and measured by atomic absorption spectrophotometry, in an airacetylene flame, at 324.7 nm. The results from 7 laboratories were satisfactory and no unfavorable comments were received. Repeatability standard deviations ranged from 0.005 to 0.023, and reproducibility standard deviations ranged from 0.012 to 0.062. When compared with the bathocuproine method, the difference in bias between both methods is not significant. They were both adopted as full CIPAC methods, with the bathocuproine method as the referee method. Both methods have been adopted official first action.

Comparison of AOAC and Atomic Absorption Spectrophotometry Methods for Determining Sodium in Fertilizers: Collaborative Study

1983 ◽  
Vol 66 (5) ◽  
pp. 1234-1241
Author(s):  
Luis F Corominas ◽  
Roberto A Navarro ◽  
Pedro Rojas ◽  
◽  
M Aihara ◽  
...  
Keyword(s):  
Atomic Absorption ◽  
Collaborative Study ◽  
Atomic Absorption Spectrophotometry ◽  
Sodium Content ◽  
Matched Pairs ◽  
High Potassium ◽  
Absorption Spectrophotometry ◽  
Low Sodium ◽  
Aoac Method ◽  
Aas Method

Abstract A collaborative study was carried out to compare AOAC method 2.147-2.150 for determining sodium in fertilizers with the atomic absorption spectrophotometric (AAS) method. Twelve synthetic NPK samples, 6 pairs of blind duplicates (0.06-8.5% Na), were sent to 22 collaborators; a total of 13 sets of results were received and evaluated by the modified matched pairs technique. The AAS method showed better performance in samples with low sodium content and high potassium content. The AAS method has been adopted official first action for determining sodium in fertilizers.

Optimization and in-house validation of the TDA-AAS method for mercury control in water and wastewater treatment plant sludges

2020 ◽  
Vol 12 (45) ◽  
pp. 5503-5513
Author(s):  
Salomé Fletcher ◽  
André Miranda ◽  
João Paiva ◽  
Maria João Benoliel ◽  
Cristina M. M. Almeida
Keyword(s):  
Thermal Decomposition ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Atomic Absorption Spectrophotometry ◽  
Water And Wastewater Treatment ◽  
Absorption Spectrophotometry ◽  
Water And Wastewater ◽  
Aas Method ◽  
Mercury Control

The validated thermal decomposition–amalgamation-atomic absorption spectrophotometry method as an adequate tool for the control of mercury in sludges.

scholarly journals DETEKSICEMARAN BAKTERIOLOGIS DAN LOGAM BERAT DAGING LANGKITANG (Faunus ater)

2020 ◽  
Vol 6 (1) ◽  
pp. 14-20
Author(s):  
yosmed Hidayat ◽  
◽  
Erismar Amri
Keyword(s):  
Heavy Metal ◽  
Microbial Contamination ◽  
Atomic Absorption Spectrophotometry ◽  
Most Probable Number ◽  
Dilution Method ◽  
Probable Number ◽  
Absorption Spectrophotometry ◽  
Bacteriological Testing ◽  
Lead Metal ◽  
Serial Dilution Method

This study aims to determine the level of bacteriological contamination and heavy metal lead (Pb) in langkitang (Faunus ater) meat. The sample were taken randomly as 15 samples from traders. Examination of microbial contamination in meat used a serial dilution method Most Probable Number (MPN) consisting of the presumptive test, confirmed test, and completed test. Measurement of Lead Heavy Metal (Pb) content used Atomic Absorption Spectrophotometry (AAS). The result of bacteriological testing of the MPN/ 100 ml sample showed that the tested Langkitang meat contained contaminating bacteria for each sample tested, and Lead metal was also detected, some of which had values that exceeded the threshold. Based on the results of the analysis, the langkitang meat has a microbial and lead metal contamination content that exceeds the health threshold so that it takes proper processing before the meat is consumed

scholarly journals Risk Factors for Bacterial Contamination of Bovine Meat during Slaughter in Ten Indonesian Abattoirs

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Diyantoro ◽  
Dhandy Koesoemo Wardhana
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Bacterial Contamination ◽  
Microbial Contamination ◽  
Visual Observation ◽  
Plate Count ◽  
Meat Production ◽  
Laboratory Examination ◽  
Beef Meat ◽  
Total Plate Count

Provision of beef meat which does not exceed the maximum microbial contamination limit is expected to meet the requirements to obtain safe, healthy, wholesome, and halal beef. Bacterial contamination during slaughtering process is a safety problem and concern for shelf life in meat production. This study was designed to determine the value of microbial contamination and its risk factors at the stage of the slaughtering process in the abattoirs. This research was conducted by visual observation accompanied by questionnaires and laboratory examination for bacterial contamination testing. The results showed the factor that significantly affected the total plate count (TPC) was carcass cutting (mean: 0.46 × 106 CFU/g; p=0.035) which was not carried out by the abattoir. The factor that had the greatest effect on the MPN of Escherichia coli was blood removal on the floor position (mean: 40.34 × 106 CFU/g; p=0.039) while the factors that significantly affected Staphylococcus aureus contamination were blood removal on the floor position (mean: 52.88 × 106 CFU/g; p=0.025) and carcass cutting which were not carried out by the abattoir (mean: 66.42 × 106 CFU/g; p=0.015).

Phytoremediation of zinc polluted soil using sunflower (Helianthus annuus L.)

2021 ◽  
pp. 9-12
Author(s):  
Elda Soares ◽  
Abdul Hamid ◽  
Sarwoko Mangkoedihardjo
Keyword(s):  
Helianthus Annuus ◽  
Research Method ◽  
Atomic Absorption Spectrophotometry ◽  
Polluted Soil ◽  
Ex Situ ◽  
Garden Soil ◽  
Sunflower Seeds ◽  
Absorption Spectrophotometry ◽  
Soil Media ◽  
Aas Method

This study aimed at assessing the ability of sunflower to remediate zinc (Zn) impacted soils and the partitioning factors of Zn in soil-plants interactions. The research method used ex situ experiments using teddy bear sunflower seeds with 8 different treatments for each. This began with the preparation of the sample soil media on garden soil, and the seeding of sunflower seeds. This research was carried out for six months starting in August - December 2020, with Helianthus annuus plants with variations of the Zn. Various concentrations of Zn were applied, ranging from 50 ppm to 350 ppm. Laboratory analysis of Zn was carried out using the Atomic Absorption Spectrophotometry (AAS) method. The research results have revealed that roots have the ability to translocate more Zn than shoots, which suggests a phytoremediation mechanism. Sunflower plants are able to translocate Zn, which proves it reliable for phytoremediation of Zn-polluted soil.

scholarly journals Microbiological and formaline test on the big eye tuna (Thunnus obesus Lowe, 1839) from fish auction place (TPI) and moving fish trader (PIK) in Panimbang Pandeglang Village Banten

2021 ◽  
Vol 1 (1) ◽  
pp. 38-45
Author(s):  
Lia Amelia Pertiwi ◽  
Hadi Susilo ◽  
Nurullah Asep Abdilah
Keyword(s):  
Microbial Contamination ◽  
Brilliant Green ◽  
Economic Value ◽  
Plate Count ◽  
E Coli ◽  
Plate Count Agar ◽  
Total Plate Count ◽  
Thunnus Obesus ◽  
The World

Big Eye Tuna (Thunnus obesus Lowe, 1839) is one of the fish species that can increase sources of animal protein and has high economic value in the world of trade because it is the second-largest export commodity after shrimp. The purpose of this study was to test the content of microbial and formalin contamination in the flesh of T. obesus fish from the Fish Auction Place (TPI) and Mobile Fish Trader (PIK) in Panimbang Village, Pandeglang, Banten. The research was carried out at the Regional Technical Implementation Unit (UPTD) Testing and Application of Quality of Fishery Products, Department of Marine Affairs, and Fisheries of Banten Province. This research is a descriptive laboratory study with purposive sampling. Twelve samples of T. obesus fish obtained from TPI (6 fishes) and PIK (6 fishes) were taken for 25 g of meat. The tested for microbial contamination content with Total Plate Count (TPC) using Butterfield's phosphate (BFP) media, and Plate Count Agar (PCA), Coliform-Test, and E. coli-Test using Lauryl Tryptose Broth (LTB). Brilliant Green Lactose Bile (BGLB), EC Broth and Levine's Eosin Methylene Blue (LEMB), and Formaldehyde-Test using  Formaldehyde-Test Kits. The results showed that the flesh of T. obesus fish contained microbial contamination with the average values ​​of TPC, Coliform MPN, and E. coli MPN, respectively, namely 1.6 103 colony/g, 15.2 MPN/g and < 3 MPN/g ( TPI), and 1,7103 colony/g, 61.3 MPN/g and < 3 MPN/g (PIK). Therefore, fish in TPI and PIK are safe for consumption as stipulated in SNI.

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