scholarly journals The Screening of Antioxidant Activities of Meniran Plants (Phyllanthus niruri Linn) and Guava Leaves (Psidium guajava Linn) Combination Using DPPH Free Radical Method

Biomedika ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 104-110
Author(s):  
Wimpy Wimpy ◽  
Tri Harningsih ◽  
Yesti Nanda Sisvitara
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Health Science ◽  
Cellular Damage ◽  
Ic50 Value ◽  
Radical Method ◽  
Guava Leaves ◽  
Guava Leaf

Degenerative disease is a serious health problem and causes a lot of deaths in Indonesia. One of the most dangerous degenerative diseases is cancer. Free radicals play a role in oxidative stress in the later stages of carcinogenesis. Antioxidant delays or inhibits cellular damage mainly through its free radical scavenging property. Meniran plants and guava leaves have high radical-scavenging activities. The previous studies reported that the combination of ginger and Meniran plant extract has a stronger antioxidant activity than the extract of a single plant. This research aims at determining the antioxidant activities of Meniran plants and guava leaves compared with its singular form. This research was conducted from October to December 2016 at the Chemical Laboratory of Nasional Health Science Institute and the Center for Development and Research of Medicinal Plants and Traditional Medicine, Tawangmangu, Karanganyar. The antioxidant activity assay was done using DPPH free radical method and vitamin C was used as the control. They were measured with UV-Vis Spectrophotometer. This study concludes that the IC50 value of Meniran plants was 30.689 ppm and the IC50 value of guava leaves was 13.7859. The IC50 values of Meniran plant and guava leaf combination with various ratios were 20.6095 ppm (1:1), 12.5629 ppm (1:2), and 16.841 ppm (2:1). The combination of Meniran plant and guava leaf extract (1: 2) had the strongest antioxidant activity of 12.56 ppm.

scholarly journals Characterization of Phytoconstituents from Alcoholic Extracts of Four Woody Species and Their Potential Uses for Management of Six Fusarium oxysporum Isolates Identified from Some Plant Hosts

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1325
Author(s):  
Mohamed Z. M. Salem ◽  
Abeer A. Mohamed ◽  
Hayssam M. Ali ◽  
Dunia A. Al Al Farraj
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Benzoic Acid ◽  
Fusarium Oxysporum ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Leaf Extracts ◽  
Conium Maculatum

Background: Trees are good sources of bioactive compounds as antifungal and antioxidant activities. Methods: Management of six molecularly identified Fusarium oxysporum isolates (F. oxy 1, F. oxy 2, F. oxy 3, F. oxy 4, F. oxy 5 and F. oxy 6, under the accession numbers MW854648, MW854649, MW854650, MW854651, and MW854652, respectively) was assayed using four extracts from Conium maculatum leaves, Acacia saligna bark, Schinus terebinthifolius wood and Ficus eriobotryoides leaves. All the extracts were analyzed using HPLC-VWD for phenolic and flavonoid compounds and the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene-linoleic acid (BCB) bleaching assays. Results: In mg/kg extract, the highest amounts of polyphenolic compounds p-hydroxy benzoic, benzoic, gallic, and rosmarinic acids, with 444.37, 342.16, 311.32 and 117.87, respectively, were observed in C. maculatum leaf extract; gallic and benzoic acids with 2551.02, 1580.32, respectively, in A. saligna bark extract; quinol, naringenin, rutin, catechol, and benzoic acid with 2530.22, 1224.904, 798.29, 732.28, and 697.73, respectively, in S. terebinthifolius wood extract; and rutin, o-coumaric acid, p-hydroxy benzoic acid, resveratrol, and rosmarinic acid with 9168.03, 2016.93, 1009.20, 1156.99, and 574.907, respectively, in F. eriobotryoides leaf extract. At the extract concentration of 1250 mg/L, the antifungal activity against the growth of F. oxysporum strains showed that A. saligna bark followed by C. maculatum leaf extracts had the highest inhibition percentage of fungal growth (IPFG%) against F. oxy 1 with 80% and 79.5%, F. oxy 2 with 86.44% and 78.9%, F. oxy 3 with 86.4% and 84.2%, F. oxy 4 with 84.2, and 82.1%, F. oxy 5 with 88.4% and 86.9%, and F. oxy 6 with 88.9, and 87.1%, respectively. For the antioxidant activity, ethanolic extract from C. maculatum leaves showed the lowest concentration that inhibited 50% of DPPH free radical (3.4 μg/mL). Additionally, the same extract observed the lowest concentration (4.5 μg/mL) that inhibited BCB bleaching. Conclusions: Extracts from A. saligna bark and C. maculatum leaves are considered potential candidates against the growth of F. oxysporum isolates—a wilt pathogen—and C. maculatum leaf as a potent antioxidant agent.

scholarly journals Cytotoxicity and Antioxidant Effects of Celastrus hindsii Benth. Leaf Extract

2020 ◽  
Vol 36 (1) ◽  
Author(s):  
Bui Thi Thanh Duyen ◽  
Vu Manh Hung ◽  
Bui Thanh Tung
Keyword(s):  
Free Radical ◽  
Cancer Cells ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Publishing House ◽  
Radical Scavenging ◽  
Antioxidant Effect ◽  
Ic50 Value ◽  
Etoac Fraction ◽  
Antioxidant Effects

Celastrus hindsii Benth et Hook. is known as a herbal medicine for the treatment of cancer. In this study we evaluated the cytotoxic and antioxidant effects of Celastrus hindsii Benth et Hook. leaf extract. Samples of Celastrus hindsii were extracted with 90 % ethanol and subsequently fractionated with n-hexane, ethyl acetate (EtOAc) and n-butanol (n-BuOH) solvents. To evaluate the cytotoxic effect, we performed MTT (3- (4,5 dimethylthiazol-2 - yl) - 2,5 - diphenyltetrazolium) assay on the three cell lines human liver Hep G2 (HB - 8065TM), lung LU-1 (HTB - 57TM), breast MCF-7 (HTB - 22TM).  The antioxidant effect was evaluated by screening DPPH (2,2-diphenyl-1-picryhydrazyl) free radical assay. The results showed that the EtOAc fraction had the strongest cytoxicity effects on liver cancer cells and lung cancer cells with an IC50 value of 33,7 ± 1,5 mg/mL and 13,0 ± 0,5 mg/mL. The BuOH fraction showed a weaker effect on lung cancer cells with IC50 value of 64,0 ± 2,2 mg/mL. The antioxidant results indicated that the EtOAc fraction had the best antioxidant effect with IC50  value of 46,9 ± 2,5 µg/mL. The EtOH total extract also has strong antioxidant activity with IC50 value of 48,5 ± 2,3 µg/mL. Our study showed that Celastrus hindsii leaf extract has the strong cytotoxicity and antioxidant activities. Keywords Celastrus hindsii Benth et Hook., cytotoxicity, MTT, antioxidant, DPPH. References [1] Ministry of health, General oncology. 2009: Vietnam Education Publishing House Limited Company, 9-10.[2] N.V. Tuyen, Pharmaceutical chemistry curriculum, 2014, Science and Technics Publishing House. 222-223.[3] V.V. Chi. Dictionary of Vietnamese medicinal plants. Medical Publishing House 1 (2012).[4] V. Gan, G. Chen, W. Zhang, J. Zhou . Oleanen induces apoptosis of cervical cancer cells by up-regulation of Bim. International Journal of Gynecologic Cancer 22(1) (2012) 38.[5] Y.H. Kuo, L.M.Y. Kuo. Antitumour and anti-AIDS triterpenes from Celastrus hindsii. Phytochemistry 44(7) (1997) 1275.[6] T. Mosmann, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays 65(1-2) (1983) 55.[7] P. Mahakunakorn, M. Tohda, Y. Murakami, K. Matsumoto, H.J.B. Watanabe, P. Bulletin, Antioxidant and free radical-scavenging activity of Choto-san and its related constituents 27(1) (2004) 38.[8] P.T. Thuong, M.K. Na, N.H. Dang, T.M. Hung, P.T. Ky, T.V. Thanh, et al. Antioxidant activities of Vietnamese medicinal plants 12(1) (2006) 29.[9] X.Q. Hu, W. Han, Z.Z. Han, Q.X. Li, X.K. Xu, P. Fu, et al. A new macrocyclic lactone and a new quinoflavan from Celastrus hindsii. Phytochemistry letters 7 (2014) 169.[10] A.C. Spivey, M. Weston, Woodhead SJCSR. Celastraceae sesquiterpenoids: biological activity and synthesis 31(1) (2002) 43.[11] T.L. Ngoc, Technology. Separation process of rosmarinic acid and their derivatives from Celastrus hindsii benth leaves. Vietnam Journal of Science 54(2C) (2016) 380.[12] F.R. Mowsumi, A. Rahaman, N.C. Sarker, B.K. Choudhury, Hossain SJWJPPS. In vitro relative free radical scavenging effects of Calocybe indica (milky oyster) and Pleurotus djamor (pink oyster). 4(07) (2015).[13] T.D. Viet, T.D. Xuan, T.M. Van, Y. Andriana, R. Rayee, H.D. Tran. Comprehensive Fractionation of Antioxidants and GC-MS and ESI-MS Fingerprints of Celastrus hindsii Leaves. Medicines 6(2) (2019) 64.[14] T.N. Ly, M. Shimoyamada, Yamauchi RJJoa, chemistry f. Isolation and characterization of rosmarinic acid oligomers in Celastrus hindsii Benth leaves and their antioxidative activity 54(11) (2006) 3786.    

scholarly journals ANTIOXIDANT ACTIVITIES OF HYDROLYSATES ORIGINATED FROM SOYBEAN AND SOY MILK RESIDUE

2018 ◽  
Vol 55 (5A) ◽  
pp. 134
Author(s):  
Ngo Minh Ngoc
Keyword(s):  
Antioxidant Activity ◽  
Protein Content ◽  
Manufacturing Industry ◽  
Antioxidant Activities ◽  
Proteolytic Enzymes ◽  
Radical Scavenging ◽  
Soy Milk ◽  
Dpph Radical ◽  
Ic50 Value ◽  
Ic50 Values

Soybean (Glycine max) and soy milk residue (okara) are protein-rich materials. Soybean possesses the highest protein content among different types of beans (protein content of soybean varies from 40–42 %). Soy milk residue, a by-product of the soy milk manufacturing industry, contains approximately 27 % protein (by dry weight). A number of recent studies have investigated the improvement of functional properties of protein contained in soybean and okara by fermentation or by the use of proteolytic enzymes. The aim of this study was to evaluate the antioxidant activities of soybean and okara hydrolysates obtained by the fermentation with Aspergillus oryzae or by using proteolytic enzymes (neutrase and flavourzyme). DPPH radical scavenging assay was used to determine the antioxidant activities of hydrolysates. The concentration of peptides required to scavenge DPPH radical by 50 % (IC50 value) was used to evaluate the antioxidant activity of peptides produced obtained from hydrolysates. The results showed that when fermented with A. oryzae, the okara hydrolysate had higher antioxidant activity than the soybean hydrolysate, with IC50 values of 0.447 mg/ml and 3.95 mg/ml, respectively. The hydrolyzed okara obtained from hydrolysis using Neutrase had higher antioxidant activity than the one obtained from hydrolysis using Flavourzyme, with IC50 values of0.200 mg/ml and 0.407 mg/ml, respectively. Different peptide fractions obtained from the hydrolysates using cut-off membrane (10 kDa, 3 kDa and 1 kDa) possessed different antioxidant activities. The < 1 kDa peptide fraction exhibited the highest antioxidant activity with an IC50 value of 0.158 mg/ml.

EVALUATION OF ANTIOXIDANT ACTIVITY OF UNRIPE AEGLE MARMELOS CORR. FRUITS

2018 ◽  
pp. 4-7
Author(s):  
Gupta D ◽  
John PP ◽  
Kumar P ◽  
Jain J
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Aegle Marmelos ◽  
Indian Origin ◽  
Ic50 Value ◽  
Phytochemical Compounds ◽  
Free Radical Scavenging Assay

Aegle marmelos Corr. (Rutaceae), commonly known as Bael, is a tree of Indian origin, well known from ancient period andprescribed for various ailments in Ayurveda. Utilization of bael fruit in day-to-day life has a great nutritional, environmental as well ascommercial importance. Every part of Aegle marmelos including stem, bark, root, leaves, fruit and seeds at all stages of maturity possesmedicinal virtues and has been used in Ethno medicine to exploit its medicinal properties. Objective: This study was undertaken to examine theantioxidant activity of methanolic extract of Aegle marmelos unripe or half ripe fruits. Material and Methods: The antioxidant activity was doneby using DPPH free radical scavenging assay. The IC50 (The concentration of sample required to scavenge 50% of DPPH free radical) wascalculated by plotting graph between % inhibition vs concentration. The ascorbic acid was used as standard antioxidant. Result and Discussion:The IC50 value of extract and ascorbic acid was found to be 62.59μg/ml and 2.80μg/ml. The antioxidant activity found in Aegle marmelos maybe associated with their main phytochemical compounds like flavonoids, phenols and tannins. Conclusion: This activity supports that the fruitcan be used as natural antioxidant to treat free radical induced cellular damages and can also be used as adjuvant with other drugs to givesynergistic effects.

Synthesis, Characterization, Free-radical Scavenging Capacity and Antioxidant Activity of Novel Series of Hydrazone, 1,3,4-oxadiazole and 1,2,4- triazole Derived from 3,5-dimethyl-1H-pyrazole

2019 ◽  
Vol 16 (7) ◽  
pp. 712-720 ◽  
Author(s):  
Khalid Karrouchi ◽  
Saad Fettach ◽  
Smaail Radi ◽  
El bekkaye Yousfi ◽  
Jamal Taoufik ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Cancer ◽  
Ic50 Values

Background: Pyrazole is an important class of heterocyclic compound, has been shown to exhibit diverse biological and pharmacological activities such as anti-inflammatory, anti-cancer, antioxidant, etc. Methods: In this study, a series of novel 3,5-dimethyl-1H-pyrazole derivatives containing hydrazine 4a-l have been synthesized via the reaction of the 2-(3,5-dimethyl-1H-pyrazol-1-yl)acetohydrazide. All synthesized compounds have been tested for their in vitro antioxidant activities via utilization of 1,1-biphenyl-2-picrylhydrazyl (DPPH) as a free radical scavenging reagent. Results: The data reported herein indicates that compound 4k showed potential radical scavenging capacity and compounds 4f and 4g exhibited best activity for the iron binding while comparing with positive controls. Conclusion: Good activity was noted for some compounds. In particular, compound 4k showed the highest antioxidant activity with IC50 values of 22.79 ± 3.64 and 1.35 ± 0.66 μg/mL in the DPPH and ABTS tests, respectively.

Antioxidant Activities of Yinchenhao (Artemisiae Capillaris) Leaves

2020 ◽  
Vol 18 (4) ◽  
pp. 386-391
Author(s):  
Yonghun Kim ◽  
Ang Li ◽  
Junyu Wang ◽  
Wancong Yu ◽  
Fang Wan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Therapeutic Effects ◽  
Scavenging Activity

Yinchenhao (Artemisia capillaris Thunb.) is a pharmaceutical agent that not only shows therapeutic effects against hepatobiliary diseases but also offers various physiological benefits. This study examined the extraction rates of antioxidant extracts from Yinchenhao leaves using different solvents. The extraction rates using different solvents were as follows: ethanol (12.1 ± 0.87%) > water (7.7 ± 0.45%) > n-butanol (1.3 ± 0.16%) ethyl acetate > (1.3 ± 0.14%) > n-hexane (1.1 ± 0.15%). The n-butanol and ethyl acetate extracts showed higher 1,1-Diphenyl-2 picrylhydrazyl radical free radical scavenging activity, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) free radical scavenging activity and ferric ion reducing antioxidant activity, while n-hexane extracts showed weak antioxidant activity. In conclusion, Yinchenhao leaf has potential as a natural antioxidant, and n-butanol and ethyl acetate may be effective extraction solvents for studying its antioxidant activities.

Study on the Antioxidant Activities of Gel Containing Tagetes erecta (L.)

2013 ◽  
Vol 747 ◽  
pp. 741-744 ◽  
Author(s):  
Tunyaluk Limsuwan ◽  
Prapaporn Boonme ◽  
Thanaporn Amnuaikit
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Common Garden ◽  
Butylated Hydroxytoluene ◽  
Gelling Agent ◽  
Tagetes Erecta ◽  
Radical Scavenging Properties

Tagetes erecta Linn. (Asteraceae), commonly known as marigold, is a common garden plant in Thailand. It was reported that antioxidant, antimycotic, and analgesic activities were found in Tagetes erecta. In this study, the alcoholic crude extracts of flowers of Tagetes erecta were evaluated its free radical scavenging properties by using 2,2diphenyl-1-picrylhydrazyl (DPPH) free radical. Butylated hydroxytoluene (BHT) was used as standard antioxidant. Tagetes erecta extract possessed the potency of DPPH radical scavenging activity (EC50 6.56±1.46 μg/ml) more than BHT (EC50 11.06±1.75 μg/ml). Later, it was prepared in gel formulation at concentration of 0.1 % w/w Tagetes erecta extract which Carbopol Ultrez 21 or Sepigel 305 was used as a gelling agent. Propylene glycol, Uniphen P-23, triethanolamine, and distilled water were used as ingredients. The physicochemical property was evaluated in terms of physical appearances, pH, viscosity, and antioxidant activity. All formulations possessed good antioxidant activity. In addition, it has good physical appearances, and pH ranged of 4 to 6. However, the viscosity depended on a polymer type and concentration of polymer.

scholarly journals Phytochemical analysis, mineral composition and in vitro antioxidant activities of Solanum macrocarpon leaves

2016 ◽  
Vol 4 (1) ◽  
pp. 62
Author(s):  
Usunomena Usunobun ◽  
Igwe V. Chinwe
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Phytochemical Screening ◽  
Mineral Analysis

Background: The aim of this study is to determine phytochemicals and mineral composition as well as in vitro antioxidant activities of Solanum macrocarpon leaves.Methods: Qualitative phytochemical screening was carried out using standard procedures while Mineral analysis was carried out using Atomic Absorption Spectrophotometer (AAS). Solanum macrocarpon leaves were also subjected for measurement of reducing power and antioxidant/radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity).Results: Phytochemical screening revealed the presence of flavonoids, saponins, alkaloids etc. Mineral analysis showed calcium (256.60mg/100g) to be higher in concentration and copper (0.62mg/100g) least in concentration while manganese was absent. Other minerals includes magnesium (81.69mg/100g), potassium (87.22mg/100g), sodium (32.51mg/100g), iron (31.41mg/100g), zinc (1.41mg/100g). Solanum macrocarpon leaves showed maximum antioxidant activity (DPPH free radical scavenging and reducing power capacity) as the higher the concentration, the higher the antioxidant activity, thus the better the free radical scavenging potentials.Conclusion: The data from this study revealed that Solanum macrocarpon has a rich content of phytochemicals, namely, saponins, alkaloids, flavonoids as well as minerals, bioactive components that are associated with health impacts. This study also revealed that Solanum macrocarpon leaves exhibit antioxidant activity. These findings thus suggest that Solanum macrocarpon leaves could act as potent source of antioxidants.

scholarly journals Antioxidant Activity Test of 2,6-bis-(2’-furilidyn)-Cyclohexanone, ; 2,5-bis-(2’-furilidyn)-Cyclopentanone; 1,5-Difuryl-1,4-pentadien-3-one

2010 ◽  
Vol 1 (1) ◽  
pp. 38
Author(s):  
Ismi Rahmawati ◽  
Endang Sri Rejeki ◽  
Sardjiman Sardjiman
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Positive Control ◽  
Log P ◽  
Dpph Radical ◽  
Curcumin Analog ◽  
Activity Test ◽  
Activity Structure ◽  
Ic50 Value

Antioxidant is an essential compound to keep man’s health due to its function as radical scavenging. Curcumin analog compounds can function as antioxidant (Sardjiman, 2000). The aim of the experiment was to find out the antioxidant activity of 2,.6-bis-(2’-furilidin)-cyclohexanone, 2,.5-bis-(2’furilidin)-cyclopentanone, and 1,.5-difuril-1.4-pentadien-3-one compounds, and the antioxidant activity of each compound against DPPH  radical with IC50 parameter as well as the correlation of compounds structure’s activities against antioxidant. The antioxidant activity of curcumin analog compounds wereas tested against DPPH free radical. The test was conducted in 5 series of concentrations by adding 4.0 ml test solutions with 1.0 ml DPPH. The antioxidant activity against free radical was measured usingwith spectrophotometer at 517 nm wavelength and determined for the IC50 value. The experiment employed rutin as positive control. The result of the experiment showed that curcumin analog compounds have antioxidant activity with IC50 of rutin, 2,.6-bis-(2’-furilidin)-cyclohexanone, 2,.5-bis-(2’furilidin)-cyclopentanone, and 1,.5-difuril-1.4-pentadien-3-one as follows: 4.93 ppm, 22.73 ppm, 20.67 ppm, and 18.80 ppm  respectively. The highest antioxidant activity belonged to 1,.5-difuril-1.4-pentadien-3-one compound which is 18.80 ppm . Correlation of activity structure of the 3 compounds can be seen from the log p parameter and energy space of HOMO-LUMO.Key words: Antioxidant, DPPH, Analog Curcumin

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Biwa (Eriobotrya japonica (Thunb.) Lindl.) Terhadap DPPH (1,1-diphenyl-2-picrylhydrazyl)

2018 ◽  
Vol 1 (3) ◽  
pp. 086-089
Author(s):  
Marianne Marianne ◽  
Revi Septiani ◽  
Yuliana Yuliana
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Eriobotrya Japonica ◽  
Ic50 Value ◽  
Anti Oxidant ◽  
North Sumatra

Biwa (Eriobotrya japonica (Thunb.) Lindl.) dapat tumbuh dengan mudah di dataran tinggi di Sumatera Utara. Tanaman ini memiliki banyak khasiat dalam mengobati berbagai penyakit. Penelitian ini bertujuan untuk mengevaluasi aktivitas antioksidan daun E. japonica dengan menggunakan DPPH (1,1-difenil-2-pikrilhidrazil). Daun E. japonica dimaserasi menggunakan etanol 96%. Ekstrak diuji dengan metode pemerangkapan radikal bebas menggunakan DPPH. Kuersetin digunakan sebagai antioksidan standar. Ekstrak diukur pada konsentrasi 0, 20, 40, 60 dan 80 ppm dengan menggunakan spektrofotometer pada panjang gelombang 516 nm, setelah diinkubasi 15 menit dengan DPPH. Daun E. japonica memiliki nilai IC50 56,59 µg/mL sedangkan IC50 kuersetin adalah 4,36 µg/mL. Sehubungan dengan itu, daun E. japonica digolongkan sebagai antioksidan kuat dan berpotensi untuk dikembangkan lebih lanjut.   Biwa (Eriobotrya japonica (Thunb.) Lindl.) can grow easily in the highlands in North Sumatra. This plant can be used to treat various diseases. This research aimed to evaluate the antioxidant activity of E. japonica leaves using DPPH (1,1-diphenyl-2-pikrilhidrazil). E. japonica leaves were macerated using ethanol 96%. The extract was evaluated by free radical scavenging method using DPPH. Quercetin was used as standard antioxidant.  The extract was measured with concentrations of 0, 20, 40, 60 and 80 ppm using spectrophotometer at 516 nm wavelength, after incubated with DPPH for 15 minutes. E. japonica leaves showed the IC50 value of 56,59 µg/mL, meanwhile, the IC50value of quercetin was 4,36 µg/mL. According to the result, E. japonica leaf was categorized as strong anti-oxidant and can be further developed

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