scholarly journals Phytotoxic effect of chrome liquor on growth and chlorophyll content of Spirodela polyrrhiza L. Schleid

2013 ◽  
Vol 5 (1) ◽  
pp. 165-170 ◽  
Author(s):  
Asha Singh ◽  
Piyush Malaviya
Keyword(s):  
Chlorophyll A ◽  
Tannery Effluent ◽  
Dependent Manner ◽  
Chlorophyll B ◽  
Fresh Weight ◽  
Fresh Biomass ◽  
Physiological Conditions ◽  
Effluent Concentration ◽  
Spirodela Polyrrhiza ◽  
Phytotoxic Effect

The present study assessed the tolerance of Spirodela polyrrhiza (L.) Schleid (giant duckweed) exposed to different concentrations (5% -100%) of chrome liquor for 7 days. Physiological conditions of S.polyrrhizaplants were monitored daily by measuring fresh weight and chlorophyll-a and chlorophyll-b. Fresh biomass of Spirodela plant showed concentration and duration dependent reduction with minimum value (5.71 g) reported on 7 d at 100% tannery effluent concentration. Likewise, significant reduction in chlorophyll-a and chlorophyll-b content wasobserved in concentration-duration dependent manner with maximum reduction reported to be 99.8% and 99.6% respectively on 7 d at 100% effluent concentration in comparison to control.

scholarly journals Preparation of Chlorophyll Nanoemulsion from Pomelo Leaves and Its Inhibition Effect on Melanoma Cells A375

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1664
Author(s):  
Man-Hai Liu ◽  
Yi-Fen Li ◽  
Bing-Huei Chen
Keyword(s):  
Chlorophyll A ◽  
High Performance ◽  
Biological Activities ◽  
Storage Period ◽  
Melanoma Cells ◽  
Inhibition Mechanism ◽  
Dependent Manner ◽  
Chlorophyll B ◽  
Caspase 9 ◽  
Concentration Dependent Manner

Pomelo (Citrus grandis), an important fruit crop grown in tropical and subtropical areas, is cultivated mainly in Asian countries. The dominant pigment in pomelo leaves, chlorophyll, has been reported to possess many biological activities such as antioxidant, anti-inflammation and anticancer. The objectives of this study were to determine chlorophylls in Pomelo leaves by high-performance liquid chromatography-mass spectrometry (HPLC-MS) and to encapsulate the isolated chlorophylls from preparative column chromatography into a nanoemulsion system for elucidating the inhibition mechanism on the growth of melanoma cells A375. The results showed that chlorophyll a and chlorophyll b could be separated within 25 min by using a C18 column and a gradient ternary mobile phase of acetone, acetonitrile and methanol. Pomelo leaves mainly contained chlorophyll a (2278.3 μg/g) and chlorophyll b (785.8 μg/g). A highly stable chlorophyll nanoemulsion was prepared with the mean particle size being 13.2 nm as determined by a dynamic light scattering (DLS) method. The encapsulation efficiency of chlorophyll nanoemulsion was 99%, while the zeta potential was −64.4 mV. In addition, the chlorophyll nanoemulsion possessed high thermal stability up to 100 °C and remained stable over a 90-day storage period at 4 °C. Western blot analysis revealed that chlorophyll nanoemulsion and extract could upregulate p53, p21, cyclin B and cyclin A as well as downregulate CDK1 and CDK2 in a concentration-dependent manner for inhibition of melanoma cells A375. Furthermore, chlorophyll nanoemulsion and extract could upregulate Bax and cytochrome C and downregulate Bcl-2, leading to activation of caspase-9, caspase-8 and caspase-3 for the induction of cell apoptosis. Compared to chlorophyll extract, chlorophyll nanoemulsion was more effective in inhibiting the growth of melanoma cells A375.

scholarly journals Common milkweed (Asclepias syriaca L.) response to sulcotrione

2017 ◽  
Vol 32 (3-4) ◽  
pp. 197-203 ◽  
Author(s):  
Jelena Gajic-Umiljendic ◽  
Marija Saric-Krsmanovic ◽  
Ljiljana Santric ◽  
Ljiljana Radivojevic
Keyword(s):  
Chlorophyll A ◽  
Physiological Parameters ◽  
Chlorophyll B ◽  
Fresh Weight ◽  
Asclepias Syriaca ◽  
Laboratory Bioassay ◽  
Application Rates ◽  
Surfactant Inhibition ◽  
Concentration Series ◽  
Moderate Susceptibility

A laboratory bioassay was conducted to investigate common milkweed response to sulcotrione. Sulcotrione was applied in concentration series of 0.15-0.90 kg a.i./ha without a surfactant and with Dash? at 1 L/ha. Plants grew for 14 days, upon which period morphological (height and fresh weight) and physiological parameters (content of carotenoids, chlorophyll a and chlorophyll b) were measured. Visual crop injury was estimated 7 and 14 days after treatment. Sulcotrione caused leaf bleaching and reduction in pigments content in common milkweed leaves and the degree of change depended on application rates and whether it was applied with or without the surfactant. Inhibition was slightly higher in plants which were treated with a combination of herbicide and surfactant. Based on the findings in this study, common milkweed showed moderate susceptibility to the recommended field rates of sulcotrione.

scholarly journals Leaf phenolics and pigments along with their antioxidative potential in some medicinal plants

2018 ◽  
Vol 2 (2) ◽  
pp. 86-92
Author(s):  
Rumana Islam ◽  
Md Injamum Ul Hoque ◽  
Md Ashrafuzzaman ◽  
Md Nesar Uddin
Keyword(s):  
Medicinal Plants ◽  
Chlorophyll A ◽  
Total Phenolics ◽  
Radical Scavenging ◽  
Chlorophyll B ◽  
Fresh Weight ◽  
Adhatoda Vasica ◽  
Paederia Foetida ◽  
Phenolics Content ◽  
Boerhavia Diffusa

Medicinal plants play significant role in human health. Besides this all of the Unani, Herbal, Kobiraji, and Homeopath medicine are produced from medicinal plant. The aim of the present study was to evaluate the phytochemical constituents as total phenolics content, free radical scavenging capacity, chlorophyll a, chlorophyll b, total chlorophyll (a+b) and carotenoids. Leaves of seven important non-solanaceous medicinal plants namely Boerhavia diffusa (punarnava), Adhatoda vasica (basok), Paederia foetida (gandhabhaduli), Rauwolfia serpentina (sarpogandha), Catharanthus roseus (pink nayantara), Catharanthus alba (white nayantara) and Andrographis paniculata (kalomegh) were extracted for phenolics, chlorophyll a and b as well as carotenoids in this experiment. Total phenolics content in seven medicinal plants in this experiment ranged from 142.44 (C. roseus) to 416.25 (A. vasica) mg GAE/100 g leaf fresh weight. The IC50 value for scavenging 2, 2- diphenyl-1- picrylhydrazyl (DPPH) free radicals ranged from 32.12 (C. roseus) to 38.40 (Boerhavia diffusa) mg mL-1 leaf extract in seven different medicinal plants. Pigments content such as chlorophyll a, chlorophyll b, chlorophyll (a+b) and carotenoids were found highest in Adhatoda vasica leaf as 2.347, 0.900, 3.283, and 0.521 mg g-1 fresh weight, respectively, among all the seven species. We may get highly valuable medicine from Adhatoda vasica (basok) leaf for its superior biochemical constituents, which will help our next generation to treat chronic diseases. Asian Australas. J. Food Saf. Secur. 2018, 2(2), 86-92

scholarly journals Effect of IBA, NAA and their Combination on Rooting and Biochemical Parameters of Stem Cuttings in Barbados Cherry

2021 ◽  
Vol 8 (5) ◽  
pp. 147-150
Author(s):  
Alam Khan Samim ◽  
B. S. Shivakumar ◽  
Ganapathi M.
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Growth Regulators ◽  
Biochemical Parameters ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Fresh Weight ◽  
Total Chlorophyll Content

An investigation was to assess the influence of growth regulators IBA, NAA and their combination on rooting and biochemical parameters of stem cuttings in Barbados cherry. It was observed that, the least number of days (21.33) taken for root initiation, maximum percentage of rooting (80.00) was observed in cutting treated with IBA 5000 ppm followed by IBA 4500 ppm over other treatments. Significantly highest Chlorophyll-a (1.26), Chlorophyll –b (0.55) and total chlorophyll content (1.82 mg/g of fresh weight) of leaves and C : N ratio (8.74) was observed in cuttings treated with IBA 5000 ppm followed by IBA 4500 ppm over all other treatments.

scholarly journals Sorbitol-induced changes in various growth and biochemici parameters in maize

2010 ◽  
Vol 56 (No. 6) ◽  
pp. 263-267 ◽  
Author(s):  
M. Jain ◽  
S. Tiwary ◽  
R. Gadre
Keyword(s):  
Nitrate Reductase ◽  
Chlorophyll A ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Proline Content ◽  
Shoot Length ◽  
Carotenoid Content ◽  
Dependent Manner ◽  
Chlorophyll B ◽  
Maize Seedlings

Treatment of maize seedlings with different concentrations of sorbitol decreased the rate of germination substantially. Root and shoot length was also reduced by sorbitol treatment, however, decrease in root length was lower than shoot length. Incubation of leaf segments from maize seedlings grown in continuous light with sorbitol decreased the fresh weight and increased the dry weight in a concentration-dependent manner. Sorbitol treatment also reduced the total chlorophylls, chlorophyll a as well as chlorophyll b; the decrease in chlorophyll 'b' being more prominent than chlorophyll 'a', however, carotenoid content was declined marginally. Supply of sorbitol decreased the protein and RNA content; however, proline content and in vivo nitrate reductase activity (NRA) were increased. The results demonstrate an inhibitory effect of sorbitol-induced stress on overall growth in maize. Amongst the biochemical parameters analysed, chlorophyll, protein and RNA contents were declined, while proline content and nitrate reductase activity were enhanced with sorbitol treatment.

scholarly journals Responses of different okra (Abelmoschus esculentus) cultivars to water deficit conditions

2021 ◽  
Vol 16 (1) ◽  
pp. 53-63
Author(s):  
Q Ayub ◽  
S M Khan ◽  
I Hussain ◽  
K Naveed ◽  
S Ali ◽  
...  
Keyword(s):  
Plant Height ◽  
Chlorophyll A ◽  
Total Protein ◽  
Field Capacity ◽  
Dry Weight ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Fresh Weight ◽  
Total Carotenoids ◽  
Drought Conditions

A pot experiment was conducted to evaluate the adverse effects of drought on different okra cultivars and to identify the most suitable okra cultivar for growing in drought conditions. Five okra cultivars namely Pusa Green, Clemson, Sabz Pari, Pusa Swani and Mehak Pari were subjected to three drought levels i.e., control (100% Field capacity),50% and 25% Field Capacity (FC). Physiological parameters like fresh and dry weight of plant and plant height were recorded along with biochemical attributes such as chlorophyll content (a, b, total) carotenoids, total protein, proline content, and Membrane stability index (MSI%). Results showed that drought significantly reduced all the studied parameters and at maximum drought (25% FC) lowest values of fresh weight (12.42g), dry weight (1.22g), plant height (7.86cm), chlorophyll a (9.02mg/g FW), chlorophyll b (18.69mg/g FW), total chlorophyll (27.71mg/g FW), carotenoids (11.80mg/g FW), total protein (2.73mg/g FW),whereas maximum Proline (21.36μg/g FW), and MSI (72%) were observed under the same drought. The results concerning responses of okra cultivars under drought conditions showed that maximum. Fresh weight (15.25g) and Dry weight (2.74g) was observed in Pusa green while maximum Plant height (13.77cm), Chlorophyll a (14.38mg/g FW), Chlorophyll b (24.41mg/g FW), Total Chlorophyll (38.80mg/g FW), Carotenoids (18.57mg/g FW), Total Protein (5.44mg/g FW), Proline (27.78μg/g FW), and MSI (56.33%) were produced by Sabz Pari. Hence it can be concluded that drought causes significant variation on physical and biochemical attributes of okra whereas Sabz Pari showed resistance towards the applied stress and produced better results.

scholarly journals Accumulation of ash and photosynthetic pigments in the raw material of Astragalus

2020 ◽  
Vol 87-88 ◽  
pp. 76-86
Author(s):  
Oleksandr Bondarchuk ◽  
Olena Vergun ◽  
Oksana Shymanska ◽  
Valentyna Fishchenko ◽  
Dzhamal Rakhmetov
Keyword(s):  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Botanical Garden ◽  
Raw Material ◽  
Chlorophyll B ◽  
Fresh Weight ◽  
National Academy Of Sciences ◽  
Calcium Phosphorus ◽  
Plant Raw Material ◽  
Academy Of Sciences

Five Astragalus species (A. cicer, A. falcatus, A. galegiformis, A. glycyphyllos, and A. ponticus) grown at the M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine were investigated. Plant raw material was collected at different ontogenetic stages, including budding, flowering, and fruiting. After that, the content of ash, calcium, phosphorus, nitrogen, and photosynthetic pigments was analyzed and statistically processed.The content of ash varied from 3.12 to 5.22 %, calcium – from 0.31 to 0.91 %, phosphorus – from 0.07 to 0.14 %, and nitrogen – from 1.93 to 2.48 % depending on the stage of growth. The content of chlorophyll a varied from 0.901 to 2.475 mg/g FW (fresh weight), chlorophyll b – from 0.190 to 0.676 mg/g FW, and carotenoids – from 0.496 to 1.129 mg/g FW.Thus, the accumulation of ash and its components in the raw material of the investigated species depended on the period of growth and certain species. Content of photosynthetic pigments correlated with the content of phosphorus (r = 0.887–0.999) and with plant increment (r = 0.756–0.998) for all investigated species. Obtained data can be applied to analyze productivity and during the cultivation of these plants.

Enhancement of physiological and biochemical attributes of okra by application of salicylic acid under drought stress

2020 ◽  
pp. 113-119
Author(s):  
Qasim Ayub ◽  
Shah Masaud Khan ◽  
Abid Mahmood ◽  
Naveed Ul Haq ◽  
Shujaat Ali ◽  
...  
Keyword(s):  
Drought Stress ◽  
Chlorophyll A ◽  
Total Protein ◽  
Electrolyte Leakage ◽  
Dry Weight ◽  
Chlorophyll B ◽  
Fresh Weight ◽  
Total Carotenoids ◽  
Effects Of Drought ◽  
Physiological And Biochemical

Horticultural crops especially vegetables are most vulnerable to drought due to their higher irrigation needs. Hence, this study was undertaken to evaluate the adverse effects of drought stress and the beneficial effects of salicylic acid (SA) on physiological and biochemical attributes of okra plants under drought stress. For this purpose, a pot experiment was laid out in Complete Randomized Design (CRD) design. Okra seeds were primed with four different SA treatments i.e., 0 (control), 1, 2 and 3 mM and sown in pots (Ø 20 cm). After 14 days of germination, the plants were subjected to two drought levels i.e., 25% and 50% field capacity (FC) and after 20 days of germination regular foliar sprays of SA at 7 days interval were performed with aforementioned SA levels. Physiological parameters like fresh weight, dry weight, and length of plants along with biochemical attributes like chlorophyll (‘a’, ‘b’ and total), total carotenoids, total protein and proline contents, and electrolyte leakage were recorded. Results revealed that drought stress (25% FC) significantly reduced all the studied parameters and resulted in the lowest values of fresh weight (5.04 g), dry weight (1.33 g), length of plants (11.68 cm), chlorophyll ‘a’ content (5.97 mg/g FW), chlorophyll ‘b’ content (8.86 mg/g FW), total chlorophyll (14.84 mg/g FW), total carotenoids (4.96 mg/g FW) and total protein (1.05 mg/g FW), except proline content (6.81 mg/g FW) and electrolyte leakage (77.31%) which was increased. Application of SA under drought stress reduced the harmful effects of drought and application of 2 mM SA produced the maximum fresh weight (8.60 g), dry weight (2.51 g), length of plants (16.23 cm), chlorophyll ‘b’ content (14.47 mg/g FW), total protein (3.73 mg/g FW) and proline content (5.11 mg/g FW); whereas application of 3 mM SA showed the highest values of chlorophyll ‘a’ (8.91 mg/g FW), total chlorophyll content (23.20 mg/g FW) and carotenoids (7.93 mg/g FW), and the lowest value for electrolyte leakage (62.00%).

scholarly journals Ecophysiological and Biochemical Changes in Abelmoschus esculentus (L.) Moench under Chromium Stress

2013 ◽  
Vol 7 ◽  
pp. 35-48
Author(s):  
P. Unnikannan ◽  
P. Vedhanarayanan ◽  
P. Sundaramoorthy
Keyword(s):  
Agricultural Land ◽  
Dry Weight ◽  
Abelmoschus Esculentus ◽  
Tannery Effluent ◽  
Fresh Weight ◽  
Gradual Reduction ◽  
Protein Amino Acid ◽  
Chlorophyll Contents ◽  
Phytotoxic Effect ◽  
Chromium Stress

Increasing concentrations of chromium caused reduction in growth, chlorophyll contents and mineral mutrients of bhendhi. At increasing concentrations of chromium, all the attributes were found to be reduced. Chromium is one of the toxic elements discharging from tannery industry. The long disposal of tannery effluent to the agricultural land causes serious damages in the soil texture; reduce the growth of microorganisms and agricultural crops. Large areas of Vellore district of Tamilnadu was seriously affected by the tannery effluent irrigation to the agricultural land. The present study was conducted to identify the phytotoxic effect and physiological changes of Abelmoschus esculentus (L.) Moench under the treatment of different concentrations of chromium solutions (2.5, 5, 10, 25, 50, 75 and 100 mg/l). The result shows that there was a gradual reduction in the morphological parameters (root length, shoot length, fresh weight and dry weight). Similarly the photosynthetic pigments (Chlorophyll and carotenoid) and biochemical parameters such as protein, amino acid, and sugars were also gradually decreased with increasing concentrations of chromium.

Etude sur la teneur en pigment de frênes qui croissent dans des conditions différentes

1973 ◽  
Vol 37 ◽  
Author(s):  
N. Lust
Keyword(s):  
Chlorophyll A ◽  
Strong Influence ◽  
The Other ◽  
Pigment Content ◽  
Chlorophyll B ◽  
Light Quantity ◽  
Yellow Pigments ◽  
The One ◽  
Green Pigments

Pigment content of ashes grown up under different circumstances - The pigment content (chlorophyll a, chlorophyll b,  xanthophyll and carotene) has been researched with ashes grown up under  different light circumstances and varying in age and height.     The results prove that the general laws concerning the influence of light  on the pigment content, don’t always work.     The phenomen is very complex. The light quantity is very important in some  cases, but insignificant in others. It seems origin and height of plants have  a strong influence. The results prove also the influence of the environment  is much higher on small plants as on big ones.     The research indicates finally the correlation between the green pigments,  the yellow pigments, and between the green pigments on the one side and the  yellow ones on the other side.

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