scholarly journals Chemomicrobiome analysis of the ornithine molecule

2021 ◽  
pp. 126-131
Author(s):  
I. Yu. Torshin ◽  
O. A. Gromova ◽  
V. A. Maksimov
Keyword(s):  
Pathogenic Bacteria ◽  
Short Chain Fatty Acid ◽  
Intestinal Flora ◽  
Human Microbiome ◽  
Chain Fatty Acid ◽  
Commensal Bacteria ◽  
Prebiotic Molecules ◽  
Level 1 ◽  
Level 2 ◽  
Biosafety Level

Hepatoprotectors and prebiotic molecules that promote the growth of intestinal flora differ significantly in their effects on different representatives of the human microbiome. This work presents the results of a comparative chemomicrobiomic analysis of ornithine and reference molecules (S-ademetionine, ursodeoxycholic acid, lactulose, and fructose). For each of the studied molecules, estimates of the values of the area under the growth curve were obtained for a representative sample of human microbiota, which included 38 commensal bacteria (including bifidobacteria and lactobacilli) and the values of the minimum inhibitory concentrations (MIC) for 152 strains of pathogenic bacteria. It has been shown that ornithine, to a lesser extent than the reference molecules, stimulates the growth of pathogenic bacteria of the genera Aspergillus, Klebsiella, Pseudomonas, Staphylococcus and Candida fungi. Ornithine is also less likely to stimulate the growth of more aggressive bacteria (Biosafety Level 2) and to a greater extent less aggressive bacteria (Biosafety Level 1). By stimulating butyric and other short-chain fatty acid-producing microorganisms, ornithine can improve the profile of gut microbiota.

scholarly journals Infant Complementary Feeding of Prebiotics for the Microbiome and Immunity

Nutrients ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 364 ◽  
Author(s):  
Starin McKeen ◽  
Wayne Young ◽  
Jane Mullaney ◽  
Karl Fraser ◽  
Warren C. McNabb ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Human Milk ◽  
Gut Microbiome ◽  
Short Chain Fatty Acid ◽  
Complementary Feeding ◽  
Chain Fatty Acid ◽  
Commensal Bacteria ◽  
Human Milk Oligosaccharides ◽  
Immune Development ◽  
Solid Foods

Complementary feeding transitions infants from a milk‐based diet to solid foods, providing essential nutrients to the infant and the developing gut microbiome while influencing immune development. Some of the earliest microbial colonisers readily ferment select oligosaccharides, influencing the ongoing establishment of the microbiome. Non‐digestible oligosaccharides in prebiotic‐supplemented formula and human milk oligosaccharides promote commensal immune‐modulating bacteria such as Bifidobacterium, which decrease in abundance during weaning. Incorporating complex, bifidogenic, non‐digestible carbohydrates during the transition to solid foods may present an opportunity to feed commensal bacteria and promote balanced concentrations of beneficial short chain fatty acid concentrations and vitamins that support gut barrier maturation and immunity throughout the complementary feeding window.

ANALISIS KEMAMPUAN KOMUNIKASI MATEMATIS DALAM MENYELESAIKAN MASALAH STATISTIKA

2018 ◽  
Vol 5 (2) ◽  
pp. 7 ◽  
Author(s):  
Ahmad Aunur Rohman
Keyword(s):  
Level 1 ◽  
Level 2

Penelitian ini dimaksudkan untuk mengetahui bagaimana kemampuan komunikasi matematis mahasiswa terhadap pemahaman statistika. Data dalam penelitian ini berupa hasil pekerjaan tes tertulis tentang kemampuan komunikasi matematis dan wawancara terhadap subjek penelitian. Pengumpulan data diperoleh dengan tes dan wawancara. Uji keabsahan data yang digunakan adalah triangulasi. Data penelitan yang terkumpul dianalisis dengan analisis data non statistik yang terdiri dari tiga alur, yaitu reduksi data, penyajian data, dan penarikan kesimpulan/verifikasi data. Hasil penelitian menunjukkan bahwa 1) Terdapat 5 mahasiswa yang berada pada level 0 (sangat kurang baik); 2) 24 mahasiswa berada pada level 1 (kurang baik); 3) 6 mahasiswa berada pada level 2 (cukup baik); Penelitian ini diharapkan dapat memacu individu lain untuk melakukan penelitian yang lebih baik dan mendalam tentang kemampuan komunikasi matematis.

EVALUASI PELATIHAN DI PT. PUPUK KUJANG CIKAMPEK

2018 ◽  
Vol 3 (2) ◽  
Author(s):  
Lania Muharsih ◽  
Ratih Saraswati
Keyword(s):  
Training Evaluation ◽  
Work Productivity ◽  
Learning Behavior ◽  
Level 3 ◽  
Level 1 ◽  
Four Levels ◽  
A Company ◽  
Level 2 ◽  
Evaluation Sheet ◽  
Assessment Factor

This study aims to determine the training evaluation at PT. Kujang Fertilizer. PT. Pupuk Kujang is a company engaged in the field of petrochemicals. Evaluation sheet of PT. Fertilizer Kujang is made based on Kirkpatrick's theory which consists of four levels of evaluation, namely reaction, learning, behavior, and results. At level 1, namely reaction, in the evaluation sheet is in accordance with the theory of Kirkpatrick, at level 2 that is learning should be held pretest and posttest but only made scale. At level 3, behavior, according to theory, but on assessment factor number 3, quantity and work productivity should not need to be included because they are included in level 4. At level 4, that is the result, here is still lacking to get a picture of the results of the training that has been carried out because only based on answers from superiors without evidence of any documents.   Keywords: Training Evaluation, Kirkpatrick Theory.    Penelitian ini bertujuan mengetahui evaluasi training di PT. Pupuk Kujang. PT. Pupuk Kujang merupakan perusahaan yang bergerak di bidang petrokimia. Lembar evaluasi PT. Pupuk Kujang dibuat berdasarkan teori Kirkpatrick yang terdiri dari empat level evaluasi, yaitu reaksi, learning, behavior, dan hasil. Pada level 1 yaitu reaksi, di lembar evaluasi tersebut sudah sesuai dengan teori dari Kirkpatrick, pada level 2 yaitu learning seharusnya diadakan pretest dan posttest namun hanya dibuatkan skala. Pada level 3 yaitu behavior, sudah sesuai teori namun pada faktor penilaian nomor 3 kuantitas dan produktivitas kerja semestinya tidak perlu dimasukkan karena sudah termasuk ke dalam level 4. Pada level 4 yaitu hasil, disini masih sangat kurang untuk mendapatkan gambaran hasil dari pelatihan yang sudah dilaksanakan karena hanya berdasarkan dari jawaban atasan tanpa bukti dokumen apapun.   Kata kunci: Evaluasi Pelatihan, Teori Kirkpatrick.

scholarly journals Celebrate Diversity?

2021 ◽  
Author(s):  
Christian Huber ◽  
Philipp Nicolay ◽  
Simone Weber
Keyword(s):  
Eine Hohe ◽  
Soziale Integration ◽  
Level 1 ◽  
Soziale Ausgrenzung ◽  
Level 2

ZusammenfassungSchulleistungen und soziale Integration sind im deutschen Schulsystem eng miteinander verbunden. Unklar ist, welche Rolle die Leistungs- und Verhaltensheterogenität für diesen Zusammenhang spielt. Vertreter*innen eines klassischen sozialpsychologischen Zugangs sagen für eine hohe Leistungs- und Verhaltensheterogenität eine Erhöhung des Risikos auf soziale Ausgrenzung (Risikohypothese) voraus, Vertreter*innen eines inklusionspädagogischen Zugangs eine Schutzfunktion (Schutzhypothese). Die vorliegende Studie untersucht, inwieweit die Leistungs- und Verhaltensheterogenität den Zusammenhang zwischen einem Förderbedarf in den Bereichen Lernen/Verhalten und sozialer Integration moderiert und ob die Richtung dieses Modertoreffekt im Sinne der Schutz- oder der Risikohypothese gedeutet werden kann. Hierzu wurden N = 1352 Grundschüler*innen in drei unterschiedlich heterogenen Schulsystemen (jahrgangsgetrennt, 1/2/3/4; teilweise jahrgangsübergreifend, 1–2/3–4; jahrgangsübergreifend, 1–4) in einer Querschnittstudie untersucht. Erhoben wurden, neben der sozialen Integration, Informationen über einen besonderen Förderbedarf in den Bereichen Lernen und Verhalten und Schulleistung. Das Schulsystem ging als unabhängige Variable auf Level 2, der Förderbedarf als unabhängige Variable auf Level 1 und die soziale Integration als abhängige Variable in die mehrebenenanalytische Auswertung ein. Analog zu bisher vorliegenden Befunden wurde ein signifikanter Haupteffekt beider Förderbedarfe auf die soziale Integration gefunden. Ferner zeigten vier von sechs Cross-Level-Effekten für den Förderbedarf Lernen Hinweise auf eine Gültigkeit der Schutzhypothese, kein Cross-Level-Effekt war im Sinne der Risikohypothese interpretierbar. Dieser Befund war nicht auf den Förderbedarf Verhalten übertragbar. Die Ergebnisse legen nahe, dass sich der Zusammenhang zwischen sozialer Integration und Förderbedarf Lernen mit zunehmender Leistungs- und Verhaltensheterogenität abschwächt. Gleichzeitig zeigte sich in den stark heterogenen Lerngruppen ein insgesamt erhöhtes Ausgrenzungsrisiko aller Schüler*innen unabhängig ihres Förderbedarfs. Die Ergebnisse lassen den Schluss zu, dass sich für Schüler*innen mit erhöhtem Förderbedarf Lernen im jahrgangsübergreifenden Lernen eine höhere Chance für eine gute soziale Integration einstellen könnte.

Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.

2021 ◽  
Author(s):  
Shiyi Lu ◽  
Deirdre Mikkelsen ◽  
Hong Yao ◽  
Barbara Williams ◽  
Bernadine Flanagan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Gut Microbiota ◽  
Plant Cell ◽  
Cell Walls ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Plant Cell Walls ◽  
Human Gut ◽  
In Vitro Fermentation

Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the...

scholarly journals Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhen Han ◽  
Peter S. Thuy-Boun ◽  
Wayne Pfeiffer ◽  
Vincent F. Vartabedian ◽  
Ali Torkamani ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Human Microbiome ◽  
Host Immunity ◽  
Rrna Gene ◽  
Metabolic Labeling ◽  
Selective Labeling ◽  
Fecal Microbiome ◽  
Acetylneuraminic Acid ◽  
Intestinal Mucus ◽  
Microbial Systems

AbstractN-Acetylneuraminic acid is the most abundant sialic acid (SA) in humans and is expressed as the terminal sugar on intestinal mucus glycans. Several pathogenic bacteria harvest and display host SA on their own surfaces to evade Siglec-mediated host immunity. While previous studies have identified bacterial enzymes associated with SA catabolism, no reported methods permit the selective labeling, tracking, and quantitation of SA-presenting microbes within complex multi-microbial systems. We combined metabolic labeling, click chemistry, 16S rRNA gene, and whole-genome sequencing to track and identify SA-presenting microbes from a cultured human fecal microbiome. We isolated a new strain of Escherichia coli that incorporates SA onto its own surface and encodes for the nanT, neuA, and neuS genes necessary for harvesting and presenting SA. Our method is applicable to the identification of SA-presenting bacteria from human, animal, and environmental microbiomes, as well as providing an entry point for the investigation of surface-expressed SA-associated structures.

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