scholarly journals Biochemical Analysis for the Determination of Renal and Pancreatic Disorders in Dogs

2012 ◽  
Vol 12 ◽  
pp. 61-68
Author(s):  
Nisha Sharma ◽  
Mukti N Shrestha
Keyword(s):  
Biochemical Analysis ◽  
Age Groups ◽  
Signs And Symptoms ◽  
Alpha Amylase ◽  
Age Group ◽  
Serum Samples ◽  
German Shepherd ◽  
Renal Disorders ◽  
Pancreatic Disorders

A study was conducted to evaluate the renal and pancreatic disorders in the dogs from September to December 2010. One hundred serum samples were collected from one hundred dogs showing the common signs and symptoms of renal and pancreatic disorders. All the serum samples were analyzed in the Laboratory of the Vet Clinic, Krishnagalli, Lalitpur. The biochemical analysis for kidney function tests was Blood urea nitrogen (BUN) and serum creatinine whereas for pancreatic disorders was alpha amylase test. The result showed the higher prevalence of both renal and pancreatic disorders together (25%) followed by pancreatic disorder which (24%) and renal disorders was (14%). Among breeds, pure breeds were found to have higher prevalence for both renal and pancreatic disorders (25.6%). Mongrels were found to have higher prevalence of pancreatic disorders (50%), and cross breeds had higher prevalence for renal disorders (38 %.) Among the pure breeds, German Shepherd had the higher prevalence. In sexwise distribution, males were more vulnerable than females. In the agewise prevalence, age groups of 5-10 were found to be more vulnerable for renal disorders followed by 10-15 and 0-5 years age group. Similarly, age groups of 10-15 were found to be highly vulnerable for pancreatic disorders followed by 5-10 and 0-5 years age group. This study, showed range value of BUN, Creatinine, and Alpha amylase were found to be 7-227 mg/dl, 0.6-41mg/dl and 112-2686 IU/L respectively.DOI: http://dx.doi.org/10.3126/njst.v12i0.6481 Nepal Journal of Science and Technology 12 (2011) 61-68 

scholarly journals Seroprevalance of Toxoplasmosis in sheep and goat: Iraq/ Sulaimania

2011 ◽  
Vol 35 (1) ◽  
pp. 16-24
Author(s):  
Lazem H. Al-Taie
Keyword(s):  
Age Groups ◽  
Neural System ◽  
Farm Animals ◽  
Economic Losses ◽  
Age Group ◽  
Serum Samples ◽  
Serological Tests ◽  
Back Ground ◽  
Qualitative Determination

Back ground: Toxoplasmosis is an important zoonosis that causes economic losses in animal herds due to abortion and stillbirth as well as changes in the reproductive and neural system of susceptible animals . Objective: The aims of the present study is to determination the prevalence of T. gondii in farm animals ( sheep& goat)of both genders and different ages in Sulaimani province by using two serological tests (ELISA and LAT). Methods: Blood samples were collected from farm animals ,142 sheep and 46 goats , of different sexes and ages. Tow different serological tests ,ELISA and LAT for qualitative determination of T. gondii antibody titer in sheep and goats serum samples. Results: The prevalence rate in sheep was 73 (51.7 %) and 82 (57 %) , and 21 (54.6 %) and 25 (54.35 %) in goats ,by ELISA and LAT respectively. The prevalence of toxoplasmosis was highest in age group 7-9 (66.6%) in sheep in compares’ with other age groups. There was no significant differences between both spp.and tow test. Conclusion: Statistical results show no significant differences between both tests (ELISA &LAT) at (P ≥ 0.05).The prevalence of toxoplasmosis was increased proportionally with the age of animals, while gender has no effect on the prevalent rate .

Influence of Age Breed and Sex on Incidence of Renal Disorders in Dogs

2021 ◽  
Author(s):  
A. Ahmad ◽  
D. Swarup ◽  
S. Dey
Keyword(s):  
Essential Role ◽  
Kidney Diseases ◽  
Age Groups ◽  
Clinical Signs ◽  
Renal Diseases ◽  
Age Group ◽  
German Shepherd ◽  
Renal Disorder ◽  
Renal Disorders ◽  
Biochemical Alterations

Background: Kidneys play an essential role in health, disease, and growth. Renal disorders are among the most common ailments of dogs and contribute substantially to canine mortality, particularly in older dogs. Fewer published reports are documenting the prevalence of renal diseases in dogs in India. The current study was undertaken to find out the incidence of renal disorder in dogs based on their age and breed and sex. Methods: The assessment of the incidence of renal disorders in dogs was done in the clinical cases reported at Referral Veterinary Polyclinic, IVRI during the period i.e. February 2010 to January 2011. The total numbers of 880 cases of dogs suffering from different ailments were reported during this period, out of which 63 dogs were suspected and screened for renal disorders based on clinical signs, ultrasonographic findings, serum and urinary biochemical alterations and urine analysis.Result: The overall incidence of renal disorders was 7.15% recorded according to the age of dogs. No renal disorders were detected in the dogs less than 6 years of age. 3.26% dogs of 6-8 year age group were confirmed for kidney diseases. Whereas 9.30% and 13.94% dogs in the age groups of 8 -10 year and ≥ 10 years, respectively had renal disorders. The breed wise renal disorders in dogs showed the highest incidence in Labrador dogs followed by Bulldogs, Dalmatian, Great Dane and Rottweiler, Doberman, German Shepherd and Pomeranian. Interestingly lowest incidence was recorded in the mixed or non-descript breed. Out of 63 dogs, 36 male (57%) and 27 female (43%) dogs were confirmed for renal disorders indicating a higher prevalence of renal diseases in males than females.

Determination of alpha-amylase activity: methods comparison and commutability study of several control materials

1995 ◽  
Vol 41 (3) ◽  
pp. 435-438 ◽  
Author(s):  
G Gubern ◽  
F Canalias ◽  
F J Gella
Keyword(s):  
Human Serum ◽  
Amylase Activity ◽  
Alpha Amylase ◽  
Human Origin ◽  
Serum Samples ◽  
Chromophore Group ◽  
Laboratory Control ◽  
Wide Range ◽  
Human Sera

Abstract Six different methods for alpha-amylase determination were compared by assaying human serum samples covering a wide range of alpha-amylase values. All the methods studied use as substrate a maltooligosaccharide with a chromophore group at the reducing end; some are chemically blocked at the nonreducing end. Intermethod comparison by regression and correspondence analyses showed significant differences for two methods. The commutability of 12 commercial control materials containing alpha-amylase was also assessed by the different methods in comparison with human serum specimens containing the pancreatic and salivary isoenzymes. We also studied the behavior of pancreatic and salivary materials prepared in our laboratory. Control materials with alpha-amylase of non-human origin were not commutable with the enzyme in human sera and should not be used for intermethod calibration.

SEASONAL AGE DYNAMICS OF EIMERIOSIS AND CRYPTOSPORIDI-OSIS OF CATTLE

2020 ◽  
Vol 3 ◽  
pp. 24-29
Author(s):  
E.S. Klimova ◽  
◽  
M.E. Mkrtchyan ◽  
E.V. Maksimova ◽  
A.D. Reshetnikova ◽  
...  
Keyword(s):  
Seasonal Dynamics ◽  
Preventive Measures ◽  
Age Groups ◽  
Central Zone ◽  
Age Group ◽  
Age Dynamics ◽  
Research Material ◽  
Age Related ◽  
Udmurt Republic

Currently, the topic of determination of the seasonal and age-related dynamics of eimeria and cryptosporidia, especially with their associative course in cattle on farms of the Udmurt Republic, remains relevant. The research material were represented by fecal samples (1629 samples) from animals of various age groups of the central zone of Udmurtia, spontaneously infected with eime-ria and cryptosporidia. Coprological studies were performed monthly by generally accepted flotation methods, and fingerprint smears were made, followed by Ziehl-Nielsen staining. Our studies on the seasonal dynamics of protozoa showed that peaks of infection in animals in the central zone of the Udmurt Republic were noted in the autumn-winter periode. The age dynamics in the regions differed significantly depending on the type of pathogen and the age of the animals. Thus, Zavyalovsky is a stationary dysfunctional area for eimeriosis and cryptosporidiosis, where the per-centage of eimeriosis infection is 96.3%. At the same time, the maximum number of ocists were observed in young animals in age of 8-12 months. Cryptosporidiosis infestation was recorded from 3 days of age to 2 months, and the percentage of infection in this age group reached 89,66%.The results of our studies revealed a fairly widespread association of these protozooses. So, it was found that in Zavyalovsky district, the maximum intensi-ty of mixtinvasion is recorded in animals aged 1-2 months and reaches up to 69,17%. In the Uvinsky district, the greatest degree of infection with the association of eimeria and cryptosporidia is record-ed almost 2 times less often (in 37,31% of cas-es).The results of seasonal dynamics must be taken into account for drawing up treatment and preventive measures for various age groups of animals in the central zone of the Udmurt Republic.

scholarly journals Study of some biochemical parameters of vitiligo patients in Bangladesh

2012 ◽  
Vol 47 (2) ◽  
pp. 173-186
Author(s):  
Md Ziaul Amin ◽  
Mohd Hafizur Rahman ◽  
Mohammed A Satter
Keyword(s):  
Uric Acid ◽  
Blood Sugar ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Age Groups ◽  
Age Group ◽  
Random Blood Sugar ◽  
Biochemical Studies ◽  
Average Serum

This study is focus on the biochemical analysis to investigate the condition of different biochemical parameters for the Vitiligo patients. This biochemical analysis deals with the blood serum level of random blood sugar, bilirubin, urea, total protein, SGOT, SGPT, alkaline phosphatase, total cholesterol and uric acids. The increasing some of the biochemical parameters were statistically significant with the age groups. Random blood sugar analysis of the 125 patient's age group 31- 40, 41- 50 and above 50 significantly higher than the control group. No significant changes were observed in serum bilirubin, serum urea, serum creatinine, serum alkaline phosphatase (ALP) and serum asparate amino transferase (AST) level in different age group of Vitiligo patients. In this study, the serum total proteins were slightly decreased in the age group patients below 10 and 11-20 as compared to healthy controls. The average serum total protein level of the below 10 and 11-20 age group patients were 5.31 g/dl and 6.50 g/dl respectively, whereas the controls were 5.95 g/dl and 6.92 g/dl respectively. Serum cholesterol, serum triglycerides and serum uric acid levels were significantly changed in the age group 11-20 and 31-40 compared with the healthy control. The average serum cholesterol level, triglyceride (TG) level and uric acid level were 152.17 mg/dl, 90.27 mg/dl, 4.75 mg/dl and 173.40 mg/dl, 149.95 mg/dl, 5.54 mg/dl respectively for the patients of 11-20 and 31-40 years age group. The controls of these parameters for the 11-20 years and 31-40 years age groups were 150.57 mg/dl, 94.41 mg/dl, 4.28 mg/dl and 156.75 mg/dl, 101mg/dl, 4.77mg/dl respectively. These biochemical studies on Vitiligo indicated that there was no significant changed observed in some biochemical parameters in the different age group of Vitiligo patients but some biochemical parameters like total protein, total cholesterol, triglycerides (TG), uric acid and random blood sugar level were significantly changed in different age group of Vitiligo patients specially among the 11-20 and 31-40 years age group. This biochemical studies would be helpful to know details about the pathological condition of the Vitiligo patients associated with other disease. DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11449 Bangladesh J. Sci. Ind. Res. 47(2), 173-186, 2012  

scholarly journals Determination of some population dynamical parameters of Squalius cappadocicus Özuluğ & Freyhof, 2011 for Melendiz River (Konya Endorheic Basin)

2020 ◽  
Vol 37 (1) ◽  
pp. 59-64
Author(s):  
Burak Seçer ◽  
Umut Cömertpay ◽  
Sevil Sungur ◽  
Erdoğan Çiçek
Keyword(s):  
Age Groups ◽  
Total Weight ◽  
Age Group ◽  
Fishing Pressure ◽  
Population Parameters ◽  
Dynamical Parameters ◽  
The Mean ◽  
Endorheic Basin ◽  
Population Mortality

The aim of this study was to determine population parameters of Squalius cappadocicus in Melendiz River. A total of 357 specimens, collected 2016 June-2017August. Age of the specimens ranged from 0th to 5th age groups and dominant age group was found I (30,53%) followed by 0th (%28,29) and 2nd (%20,44) age groups. Total length varied from 2.6-24.5 cm with the mean of 11.27±3.88 cm, total weight ranged from 0.44 to 202.99 with the mean of 23.57±25.32 g. Length-weight relationship were estimated W=0.0120*L2.9882. Estimated population parameters were calculated as L∞: 44.21 cm, k: 0.098, to: -1.47, Φꞌ: 2.28 and K: 1.13 for the population. Mortality and exploitation rates estimated as Z: 0.37, M: 0.32, F: 0.05 and E: 0.13, respectively. These values suggested that there is no over fishing pressure on the population.

scholarly journals Plasma Thymus and Activation-Regulated Chemokine (TARC) As Diagnostic Marker in Pediatric Hodgkin Lymphoma

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 5312-5312
Author(s):  
Eline Zijtregtop ◽  
Waichu Wong ◽  
Friederike Meyer- Wentrup ◽  
Martha Lopez-Yurda ◽  
Raoull Hoogendijk ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Hodgkin Lymphoma ◽  
Sensitivity And Specificity ◽  
Diagnostic Marker ◽  
Age Groups ◽  
Serum Levels ◽  
Control Group ◽  
Age Group ◽  
Blood Biomarkers ◽  
Serum Samples

Abstract Background Pediatric classical Hodgkin lymphoma (cHL) is a clonal disorder in an inflammatory background, also known as the microenvironment. This microenvironment is of major importance for growth and survival of the malignant Hodgkin/Reed Sternberg (HRS) cells. HRS cells and the microenvironment communicate through chemo- and cytokines. Blood biomarkers result from this active crosstalk, and may be a surrogate for lymphoma viability (Steidl et al, JCC 2011). Blood biomarkers are important because they hold the promise to be easily available and cost-effective. One promising biomarker in adult patients with cHL is the "Thymus and Activation-Regulated Chemokine, TARC (Plattell et al, Haematologica 2012). Elevated TARC levels are also described in patients with atopic dermatitis (Hijnen et al, J All Clin Immunol 2004). In adult cHL patients about 85% of patients have significantly elevated levels of TARC in pre-treatment serum or plasma compared to healthy controls (Plattell et al). So far nothing is known about TARC in pediatric cHL patients. To define its value as a diagnostic marker in pediatric cHL patients, we compared TARC levels of pediatric cHL patients with control patients. This study was IRB-approved and registered under Dutch Trial registry number 6876. Methods After providing informed consent, plasma and serum samples were collected of newly diagnosed cHL patients before start of treatment. To define normal values of TARC in children, samples were collected from non-cHL randomly selected patients from the hematology, endocrinology and oncology outpatient clinic. Baseline characteristics including history of atopic dermatitis were collected. These control patients were divided in three age groups (0-9,10-14 and 15-18 years). TARC levels were measured by enzyme-linked immunosorbent assay (R&D systems, Human CCL17/TARC Quantikine ELISA Kit). TARC levels of the cHL patients were compared to the control group to obtain ROC curves and calculate the AUC, cross-validated sensitivity and specificity and accuracy of TARC as a diagnostic marker. We hypothesized that pediatric cHL patients had elevated pretreatment TARC levels in both serum and plasma. Analyses were done using SAS V9.4. Results Fourteen cHL patients were included with a median age of 14 (range 11-17) years. Ten (71.4%) were female. Eighty patients were included in the control group with a median age of 12 (range 10 months-18) years. Twenty-nine patients (36.3%) were included in age group 0-9, 25 (31.2%) in age group 10-14 and 26 (32.5%) in age group 15-18. Thirty-nine (48.8%) were female. Patients of the control group had a median TARC value of 71 (range 18-762) pg/ml for plasma and 318 (range 27-1300) pg/ml for serum. TARC plasma and serum levels decreased with age (Spearman correlation -0.26, 2-tailed p=0.0204), but there were no statistically significant pairwise comparisons found between the pre-specified age groups. In the eight control patients (10%) with atopic dermatitis no significantly higher plasma and serum levels were found (plasma median with eczema 97 versus 70 pg/ml without eczema (p=0.71) and serum median with eczema 643 versus 317 pg/ml (p=0.71)). Plasma was collected in 14 cHL patients, and all had elevated TARC levels, with a median plasma level of 18449 (range 1635-55821) pg/mL. Serum samples were collected in 8/14 cHL patients and all had elevated serum TARC levels. Median serum level: 46703 (range 12817-149739) pg/ml. The plasma TARC levels of cHL patients were significantly higher than those of the control group patients (p<0.001). With a cut-off of level of of 898.70 pg/ml, we obtained 100% (95% CI 73% - 100%) sensitivity and 100% (95% 94% - 100%) specificity. Serum TARC levels also were significantly higher than those of the control group patients (p<0.001), with a cut-off level of 10283.57 pg/ml, sensitivity and specificity will be 100% (95% CI 60% - 100% for sensitivity and 95% CI 94% - 100% for specificity). Conclusion All classical cHL patients had significantly higher TARC levels compared to the 80 control patients. Despite the small sample size of cHL patients, TARC was found to be a sensitive and specific diagnostic marker for pediatric cHL in both plasma and serum. Further research with a bigger sample of cHL patients is necessary to improve the accuracy of the sensitivity, as well as to investigate whether TARC is also a valuable marker for disease response during treatment in pediatric patients with cHL. Disclosures No relevant conflicts of interest to declare.

scholarly journals Examination of Trait Anxiety States of University Students Doing Sports in Team and Individually in Terms of Some Variables

2019 ◽  
Vol 7 (8) ◽  
pp. 93
Author(s):  
Serkan Zengin
Keyword(s):  
Trait Anxiety ◽  
Age Groups ◽  
University Faculty ◽  
Age Group ◽  
National Team ◽  
Anxiety States ◽  
Anxiety Levels ◽  
Individual Sports ◽  
Sports Sciences

Research has been conducted for the students studying at Akdeniz University Faculty of Sports Sciences. In the research, it was aimed to investigate the trait anxiety states of students studying at Akdeniz University Faculty of Sports Sciences and a survey model was used in the study. This study is consisted of two parts; there are 6 items in determination of demographical variables in the first part. “Trait Anxiety Inventory” Scale consisting of 20 questions developed by Spielberger was used in the second part. Likert type four-point was used in the scale items given in the second and third parts (showing the statements “Almost Always”, “Sometimes”, “Usually”, “Almost Never”). Once the comparison of trait anxiety levels by the age groups is examined among the results of the study, it is seen that trait anxiety levels of participants differ at a statistically significant level by the age groups (p<0,05). It was concluded that the trait anxiety level of the participants in the age group of 20-21 and age group of 22-23 is significantly higher than the participants in the age group of 23+ (P<0,05). In conclusion, it is considered that those who do individual sports feel more anxiety states since the responsibility only belongs to themselves. Trainings minimizing the anxiety should be further offered to the individuals doing such sports. Individual team athletes who are at national team level may be more supported.

scholarly journals Seroprevalence and Clinical Presentation of Chikungunya Virus Infection among Febrile Outpatients Seeking Health Care in Mzuzu City, Malawi

2021 ◽  
Author(s):  
Flywell Kawonga ◽  
Gerald Misinzo ◽  
Dylo Pemba ◽  
Leonard Mboera ◽  
Isaac Thom Shawa
Keyword(s):  
Chikungunya Virus ◽  
Clinical Presentation ◽  
Age Groups ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Viral Disease ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igm Antibodies

Chikungunya is a mosquito-borne viral disease caused by Chikungunya virus (CHIKV. We conducted this study determine the seroprevalence and clinical presentation of Chikungunya infection among outpatients seeking healthcare in Mzuzu City, Malawi. Blood samples were collected from malaria negative and non-septic febrile outpatients with fevers &ge;38 &deg;C, for not more than 5 days. The enzyme- linked immunosorbent assay (ELISA) test was used to detect anti-CHIKV IgM antibodies and its results were used to determine seroprevalence of Chikungunya. A total of 119 serum samples were tested, of these, 73 (61.3%) tested positive for anti-CHIKV IgM antibodies by ELISA. Laboratory requisition forms were used to capture demographic information such as age, sex, clinical signs and symptoms presented by the enrolled patients. Age groups of 1-9, 10- 19, 20- 29, 30- 39, 40- 49, and &ge;50 years had 17.8% (n= 13), 12.3 %,( n=9), 15.1%) (n=11), 19.2%; (n=14), 17.8% (n=13) and 17.8% (n=13) proportion of seroprevalence respectively. Most of the CHIKV infected individuals presented with fever (52.05%), joint pain (45.21%) and abdominal pain (42.67%). The presence of anti- CHIKV IgM antibodies suggest the presence of recent CHIKV infection and therefore accurate laboratory assays are highly recommended for CHIKV diagnosis and appropriate management of febrile patients.

scholarly journals Seroepidemiology of Human Group C Rotavirus in Japan Based on a Blocking Enzyme-Linked Immunosorbent Assay

2001 ◽  
Vol 8 (1) ◽  
pp. 161-165 ◽  
Author(s):  
Mitsutaka Kuzuya ◽  
Ritsushi Fujii ◽  
Masako Hamano ◽  
Ritsuko Ohata ◽  
Hajime Ogura ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Age Groups ◽  
Neutralization Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Age Group ◽  
Human Group ◽  
Serum Samples ◽  
Group C Rotavirus ◽  
Group A ◽  
Okayama Prefecture

ABSTRACT A novel blocking enzyme-linked immunosorbent assay (BL-ELISA) was developed for detection of antibodies to human group C rotavirus (CHRV). The specificity of the BL-ELISA was confirmed by using animal sera hyperimmunized to group A and group C rotaviruses and paired sera from five patients with acute CHRV gastroenteritis. Furthermore, there was concordance between the BL-ELISA and a neutralization assay for CHRV in 226 (95%) of 238 samples. By using the BL-ELISA, we determined the seroprevalence of CHRV in 704 serum samples obtained from nine different age groups of inhabitants of Okayama Prefecture, Japan, in 1992, 1994, and 1996. As a result, 211 sera (30%) were found to be positive for CHRV antibodies. The seroprevalence gradually increased with age and reached 52.7% in the oldest individuals. A further analysis of the youngest age group suggested that CHRVs predominantly prevail in persons older than 3 years of age in Japan. When comparing the three sampling years, a larger percentage of antibody-positive sera was detected in 1994 than in either 1992 or 1996 in individuals between 6 and 15 years of age, reflecting the occurrence of a CHRV outbreak among children during the winter of 1992 to 1993 that was previously documented. These results indicate that CHRV infections may occur more frequently in spite of the relatively low detection rate of the virus.

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