scholarly journals The study of the ability to androgenesis in the winter soft wheat anthers

2020 ◽  
pp. 41-45
Author(s):  
S. G. Golovko ◽  
N. V. Kalinina ◽  
A. A. Yatsyna ◽  
N. N. Vozhzhova ◽  
E. V. Ionova
Keyword(s):  
Genetic Diversity ◽  
Winter Wheat ◽  
Nutrient Medium ◽  
Agricultural Research ◽  
Practical Interest ◽  
Wheat Breeding ◽  
Current Paper ◽  
Soft Wheat ◽  
Maximum Percentage

The improvement of genetic diversity and acceleration of breeding process are the most important tasks in wheat breeding. In vitro biotechnological methods are of practical interest for breeding process. The current paper has considered the effects of genotypes and nutritious medium on the formation of regenerants. The purpose of the research was to study the ability to androgenesis in winter soft wheat anthers and to identify promising winter soft wheat genotypes. The estimation of the ability to androgenesis was carried out among ten winter wheat samples. 8711 anthers were planted on medium of N6 and PII induction; the 190-2 medium was used for regeneration. As a result of the study, it was found that the process of neoplasms occurrence depends both on the variant of the nutrient medium and on the chosen genotype. It has been proven that the most favorable medium for the cultivation of winter soft wheat anthers is medium N6. It was found that the maximum percentage of neoplasms (5.21%) from winter soft wheat anthers was recorded in the sample “595/13”. It was found that the samples “595/13” (9 plants) and “Niva Dona” (6 plants) developed by the FSBSI “Agricultural Research Center “Donskoy” showed the highest responsiveness to the formation of calluses and plants-regenerants. There were obtained 6 green regenerants and 3 albino forms from the pollen callus of the sample “595/13” and 4 green regenerants and 2 albino regenerants from the sample “Niva Dona”.

Using DArT Markers to Monitor Genetic Diversity throughout Selection: A Case Study in Nebraska's Winter Wheat Breeding Nurseries

Crop Science ◽  
2013 ◽  
Vol 53 (6) ◽  
pp. 2363-2373 ◽  
Author(s):  
Ibrahim El-basyoni ◽  
P. Stephen Baenziger ◽  
Ismail Dweikat ◽  
Dong Wang ◽  
Kent Eskridge ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Winter Wheat ◽  
Wheat Breeding ◽  
Dart Markers

scholarly journals Physicochemical and rheological properties of the derivatives of Ningmai 9 wheat and their relationship with sugar-snap cookie diameter

2020 ◽  
pp. 1150-1156
Author(s):  
Pingping Zhang ◽  
Jinbao Yao ◽  
Hongxiang Ma ◽  
Guicheng Song
Keyword(s):  
Winter Wheat ◽  
Rheological Properties ◽  
Yangtze River Basin ◽  
Multiple Regression Model ◽  
Wheat Breeding ◽  
Soft Wheat ◽  
Quality Trait ◽  
Retention Capacity ◽  
Milling Quality ◽  
Deformation Work

In recent years, the annual demand for soft wheat in China has exceeded six million metric tons. The development of soft wheat is an important objective in the Yangtze River Basin winter wheat region in China. Ningmai 9 is a soft wheat cultivar widely used as a parent in the soft red winter wheat breeding program. However, the physicochemical and rheological properties of Ningmai 9 and its derivatives remain unknown. In this study, Ningmai 9 and its thirty-one derivatives were grown in Nanjing in Jiangsu Province in two successive cropping seasons from 2015 to 2017. The kernel samples were analyzed for milling quality, solvent retention capacity (SRC), alveograph parameters, and sugar-snap cookie diameter. The flour protein content was not significantly associated with any other quality trait. P, L, P/L (the ratio of tenacity to extensibility), alveograph deformation work (W), and four types of SRC were significantly correlated with sugar-snap cookie diameter. Compared to Ningmai 9, seven wheat genotypes had relatively better cookie quality and lower SRC and P values. A multiple regression model with water SRC (WSRC) as a variable explained 72.5% of the total variation in cookie diameter. These results suggest that decreasing SRC and gluten strength is the key to improving soft wheat breeding programs.

scholarly journals Genotypic features of morphogenetic reactions of varieties and hybrids F1 of winter soft wheat during various stages of androgenesis in vitro

2018 ◽  
Vol 22 ◽  
pp. 252-256 ◽  
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
M. S. Boyko
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Soft Wheat ◽  
F1 Hybrids ◽  
Green Plants ◽  
Ability To Regenerate ◽  
Androgenesis In Vitro ◽  
Soft Winter Wheat

Aim. Testing the haploproduction ability of 47 varieties and 143 F1 hybrids of winter bread wheat. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Results. The differences in the frequency of сallus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The range of variation haploproduction activiti was broad. The sign of "the formation of сallus" for varieties was in limited from 0 to 21.2 % and for hybrids – from 0 to 38.8 %; on the sign of "regeneration of green plants" for varieties – from 0 to 9.4 %, and for hybrids from 0 to 9.1 %. The positive effect of 1BL/1RS translocation on signs of haploproduction in anther culture Triticum aestivum L. Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed. It has been found that F1 hybrids have a higher potential production potential in anther culture than varieties. During the period from 2011 to 2017, the 580 lines of winter wheat were transferred to the breeding departments. Keywords: soft winter wheat, anther culture in vitro, callus, regeneration.

scholarly journals Stabilization of soft wheat selection material which is the result of crossing with wild relatives

2021 ◽  
Vol 28 ◽  
pp. 83-87
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
T. P. Nargan ◽  
M. S. Chekalova
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Callus Formation ◽  
Wild Relatives ◽  
Soft Wheat ◽  
Ability To Regenerate ◽  
Androgenesis In Vitro ◽  
Callus Regeneration ◽  
Soft Winter Wheat

Aim. Testing the haploproduction ability of 30 hybrids of winter soft wheat. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Results. The differences in the frequency of callus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The microspores of 17 of 30 hybrids formed callus by in vitro anther culture were shown. The intensity of one process was different: more than half of the genotypes (18 pcs.) were characterized by a low percentage of callus (from 0.10 to 1.0%), 6 genotypes - medium (from 1.0 to 3.0%), and three - high (4.36%; 15.11% and 15.81%, respectively). Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed Samples P26 and P27 showed the highest level of callus formation. The 10 green regenerating plants were obtained. Keywords: hybrids, soft winter wheat, anther culture in vitro, callus, regeneration.

scholarly journals ‘PODAROK KRYMU’ – NEW EARLY RIPENING VARIETY OF WINTER SOFT WHEAT

2020 ◽  
Vol 4 (24) ◽  
pp. 114-121
Author(s):  
D.M. Marchenko ◽  
◽  
M.M. Ivanisov ◽  
E.I. Nekrasov ◽  
N.S. Kravchenko ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Field Experiments ◽  
Agricultural Research ◽  
Soft Wheat ◽  
Test Plot ◽  
Average Yield ◽  
New Variety ◽  
Preceding Crop ◽  
Early Ripening ◽  
Variety Testing

Creation and introduction into production new high-yielding and early ripening varieties of soft winter wheat adapted to certain weather conditions are the principal ways to increase yield and improve general harvest volumes. A properly selected variety is one of the most effective ways of achieving crop yield improvement for any farm. To realize the potential for increasing yields, it is crucial to bear in mind specific soil and climatic conditions. The aim of the research was to characterize a new variety of winter soft wheat ‘Podarok Krymu’ by economic and biological qualities, as well as morphological characteristics. Soil of the experimental plot – chernozems ordinary with a thick humus layer (up to 140 cm). The meteorological conditions during the years of research were quite diverse, which made it possible to evaluate the new variety by a set of economically valuable features. The studies were carried out in 2015–2019 on the experimental fields of the scientific crop rotation of the Department of Winter Wheat of State Scientific Establishment “Agricultural research center “Donskoy” (SSE “ARC “Donskoy”). The object of the research – a new variety of winter soft wheat ‘Podarok Krymu’. Winter soft wheat variety ‘Don 107’ was used as a standard. Preceding crop – corn for grain. The accounting area of the variety test plot – 10 m². Field experiments were replicated six times. In 2019, variety ‘Podarok Krymu’ was submitted for State Variety Testing by SSE “ARC “Donskoy” together with the Research Institute of Agriculture of Crimea. This variety has a high potential for grain productivity. The average yield in the competitive variety testing was 7.71 t/ha (preceding crop – corn for grain), which is 0.39 t/ha higher than the average yield of standard variety ‘Don 107’. According to the length of the growing season, the variety belongs to the early maturing group; its ears emerge and kernels ripe three days earlier than that of the standard. The high and stable yield of the new variety is ensured by its tolerance to drought and heat, increased winter hardiness and resistance of plants to frost, as well as resistance to the main diseases typical in the regions where studies are being conducted. Since the autumn of 2020, ‘Podarok Krymu’ has been studied to cultivate it in the North Caucasian, Lower Volga and Central Black Earth regions of the Russian Federation.

scholarly journals НОВЫЕ ОСОБЕННОСТИ ПРОЦЕССА КЛОНАЛЬНОГО МИКРОРАЗМНОЖЕНИЯ СОРТА КАРТОФЕЛЯ СЕЛЕКЦИИ ЗАПАДНОЙ СИБИРИ

2015 ◽  
Vol 6 (01) ◽  
pp. 375-389
Author(s):  
E.P. Miakisheva ◽  
O.K. Tavartkiladze ◽  
D.A. Durnikin
Keyword(s):  
Russian Federation ◽  
Nutrient Medium ◽  
Viral Infections ◽  
Agricultural Research ◽  
Morphological Characteristics ◽  
Morphological Structure ◽  
High Yield ◽  
Indolebutyric Acid ◽  
The Russian Federation

<span lang="EN-US">The article is sanctified urgent need to use methods of modern biotechnology in primary seed farming of potatoes in the territory of the Russian Federation. Lack of required amount of good quality planting material is a major factor limiting stable high yield. The crop of potatoes, annually produced in Russia, significantly below global, and does not meet genetic capabilities of the species. Many viral infections tend to accumulate in the tubers, reducing their quality and keeping quality during storage. For the first time peculiarities of clonal micropropagation and in vitro culture of early-maturing potato varieties Lubava Russian breeding (agricultural research Institute of the city of Kemerovo, the Kemerovo region of the Russian Federation). At the stage of introduction to the culture of the parameters, providing pure cultures using lizoformin as sterilizing compounds. At the stage of actually breeding to obtain plants-regenerants of a certain morphological structure was studied the influence of certain components of the nutrient medium. The influence of agar, sucrose and vitamins such morphological indicators of development of regenerated plants as number of internodes and plant height. In the rooting stage the effect of different of growth regulators auxin nature: </span><span>α</span><span lang="EN-US">-naphthyloxy acid, </span><span>β</span><span lang="EN-US">-indoleacetic acid and </span><span>β</span><span lang="EN-US">-indolebutyric acid, in concentrations of 0.1-5 </span><span>μ</span><span lang="EN-US">m. Selected concentrations of these compounds, the introduction of which in the nutrient medium provided the maximum indices of rhisogenesis: the number and length of roots. At the stage of adaptation to non-sterile growing conditions ex vitro the efficiency of the hydroponic plants, filled with a liquid nutrient solution. A successful stage adaptation has characterizability 100% survival rate of plants and increase the morphological characteristics of regenerants.</span>

scholarly journals Androgenic responses of winter wheat (Triticum aestivum L.) combinations in in vitro anther culture

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 335-350
Author(s):  
Osama Kanbar ◽  
Csaba Lantos ◽  
Erzsebet Kiss ◽  
Janos Pauk
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Published Data ◽  
In Vitro Production ◽  
The Mean ◽  
Dh Lines ◽  
Vitro Production

The androgenic parameters were investigated by in vitro anther culture (AC) on nine F2-5 breeding combinations of winter wheat (Triticum aestivium L.). Each combination produced embryo-like structures (ELS), green plantlets, albino plantlets, transplanted plantlets and acclimatized plantlets, with respect to the number of anthers in AC. The number of AC-derived ELS was between 11.73 and 52.76 ELS/100 anthers with the mean of 26.22 ELS/100 anthers, out of which the number of regenerated green plantlets varied from 3.20 to 26.40 green plantlets/100 anthers and the mean was 9.76 green plantlets/100 anthers, while the number of transplanted plantlets ranged from 2.16 to 21.77 transplanted plantlets/100 anthers. Furthermore, the number of albinos/100 anthers was mitigated and varied between 0.72 and 6.20 albinos/100 anthers. We also studied the number of green and albino plantlets per 100 ELS. The rate of green plantlets per 100 ELS ranged between 14.81% and 64.01%, with the overall mean 33.59%, while the rate of albinos per 100 ELS ranged from 4.82% to 20.70% with the overall mean 11.93%. In our experiment, the rate of acclimatized plantlets (70.15-91.57%) depended mostly on the combination. This study asserted the importance of AC method in wheat for in vitro production of green plants. Although albinism was found in each combination, it did not hinder the production of green plantlets. The satisfying results were achieved in green plantlets production compared to the previously published data, but further improvement will be needed continuously, experiment by experiment. The generated acclimatized plantlets will be used in the wheat breeding program as doubled haploid (DH) lines.

Genetic variation within and between winter wheat genotypes from Turkey, Kazakhstan, and Europe as determined by nucleotide-binding-site profiling

Genome ◽  
2011 ◽  
Vol 54 (5) ◽  
pp. 419-430 ◽  
Author(s):  
Muge Sayar-Turet ◽  
Susanne Dreisigacker ◽  
Hans-J. Braun ◽  
Arne Hede ◽  
Ruth MacCormack ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Winter Wheat ◽  
Binding Site ◽  
Nucleotide Binding ◽  
Wheat Breeding ◽  
Nucleotide Binding Site ◽  
R Genes ◽  
Breeding Programs ◽  
Wheat Genotypes ◽  
Nbs Profiling

The genetic diversity within wheat breeding programs across Turkey and Kazakhstan was compared with a selection of European cultivars that represented the genetic diversity across eight European countries and six decades of wheat breeding. To focus the measure of genetic diversity on that relevant to disease-resistant phenotypes, nucleotide-binding-site (NBS) profiling was used to detect polymorphisms associated with the NBS motifs found within the NBS – leucine-rich repeat (LRR) class of resistance (R) genes. Cereal-specific NBS primers, designed specifically to the conserved NBS motifs found within cereal R-genes, provided distinct NBS profiles. Although the genetic diversity associated with NBS motifs was only slightly higher within the Eastern wheat genotypes, the NBS profiles produced by Eastern and European wheat lines differed considerably. Structure analysis divided the wheat genotypes into four groups, which compared well with the origin of the wheat genotypes. The highest levels of genetic diversity were seen for the wheat genotypes from the Genetic Resource Collection held in Ankara, Turkey, as wheat genotypes within breeding programs were genetically more similar. The wheat genotypes from Kazakhstan were the most similar to the European cultivars, reflecting the significant number of eastern European cultivars used in the breeding program in Kazakhstan. In general, the NBS profiles suggested that NBS–LRR R-gene usage in winter wheat breeding in Turkey and Kazakhstan differed from that deployed in European cultivars.

scholarly journals The most important selection criteria in winter wheat breeding for grain quality

2021 ◽  
Vol 4 (28) ◽  
pp. 50-57
Author(s):  
N.A. Galushko ◽  
◽  
N.I. Sokolenko ◽  
Keyword(s):  
Winter Wheat ◽  
Mass Fraction ◽  
Grain Quality ◽  
Agricultural Research ◽  
Wheat Breeding ◽  
Mineral Fertilizers ◽  
Wheat Grain ◽  
North Caucasus ◽  
The North ◽  
Sedimentation Value

The quality of wheat grain largely depends on the hereditary characteristics of the variety. In 2018-2020, in the North Caucasus Federal Agricultural Research Center, studies were carried out to search for source material among the diversity of genotypes of common winter wheat at the early stages of the breeding process to select the most promising in the context of grain quality. The soil of the experimental plot is ordinary medium-thick low-humus medium loamy chernozem. The climate of the zone is temperate continental. According to long-term data, the annual sum of effective temperatures is 3177.2 °C; average annual precipitation is 559.6 mm; Selyaninov's hydrothermal coefficient (HTC) is 1.06. Before sowing, complex mineral fertilizers were applied at a dose of N40P60K40; in spring, ammonium nitrate – 26 kg of active ingredient per ha. Fifteen lines of common winter wheat selected according to a complex of breeding valuable signs (yield, resistance to diseases, frost and winter hardiness, drought resistance) served as a material for the studies. Lines were compared with the standard variety ‘Aivina’ according to the most important criteria: gluten mass fraction and quality, protein mass fraction and sedimentation value. Four wheat lines (21663, 20029, 21728, 21944) characterized by gluten of I and II group (gluten content from 23 % to 25.7 %) were identified. They exceeded the standard by 2.0–4.7% and corresponded to the 3 class of grain quality. In line 21944, minor variability of the protein amount (10 %) in the grain by year was noted; in the lines 21226, 21924 and 20029 ‒ average (14.2‒18.7 %); in the rest genotypes, including standard, it was significant (20.1‒34.3 %). On average, over the years of studying, six lines (21420, 21663, 21683, 21118, 21944, 21924) were classified as strong wheat (sedimentation value was in the range of 51‒62 ml). The flour strength of the ‘Aivina’ wheat grain had an average sedimentation value – 49 ml. Lines 21944 and 21924, which exceeded the standard, can be used as sources of high-quality grain when creating new varieties of common winter wheat for regions with a dry period of grain formation.

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