scholarly journals НОВЫЕ ОСОБЕННОСТИ ПРОЦЕССА КЛОНАЛЬНОГО МИКРОРАЗМНОЖЕНИЯ СОРТА КАРТОФЕЛЯ СЕЛЕКЦИИ ЗАПАДНОЙ СИБИРИ

Author(s):  
E.P. Miakisheva ◽  
O.K. Tavartkiladze ◽  
D.A. Durnikin

<span lang="EN-US">The article is sanctified urgent need to use methods of modern biotechnology in primary seed farming of potatoes in the territory of the Russian Federation. Lack of required amount of good quality planting material is a major factor limiting stable high yield. The crop of potatoes, annually produced in Russia, significantly below global, and does not meet genetic capabilities of the species. Many viral infections tend to accumulate in the tubers, reducing their quality and keeping quality during storage. For the first time peculiarities of clonal micropropagation and in vitro culture of early-maturing potato varieties Lubava Russian breeding (agricultural research Institute of the city of Kemerovo, the Kemerovo region of the Russian Federation). At the stage of introduction to the culture of the parameters, providing pure cultures using lizoformin as sterilizing compounds. At the stage of actually breeding to obtain plants-regenerants of a certain morphological structure was studied the influence of certain components of the nutrient medium. The influence of agar, sucrose and vitamins such morphological indicators of development of regenerated plants as number of internodes and plant height. In the rooting stage the effect of different of growth regulators auxin nature: </span><span>α</span><span lang="EN-US">-naphthyloxy acid, </span><span>β</span><span lang="EN-US">-indoleacetic acid and </span><span>β</span><span lang="EN-US">-indolebutyric acid, in concentrations of 0.1-5 </span><span>μ</span><span lang="EN-US">m. Selected concentrations of these compounds, the introduction of which in the nutrient medium provided the maximum indices of rhisogenesis: the number and length of roots. At the stage of adaptation to non-sterile growing conditions ex vitro the efficiency of the hydroponic plants, filled with a liquid nutrient solution. A successful stage adaptation has characterizability 100% survival rate of plants and increase the morphological characteristics of regenerants.</span>

2021 ◽  
Vol 36 ◽  
pp. 07012
Author(s):  
Angelina Malkova ◽  
Ivan Evdokimov ◽  
Maxim Shirmanov ◽  
Alena Irkitova ◽  
Dina Dudnik

Data of the microbial biopreparation development for protection and crop growth stimulation on the Bacillus bacteria basis are presented. Three B. pumilus strains isolated from the Altai region (the Russian Federation) plants rhizosphere were selected as active components of the bacterial preparation. L-bulone was chosen as the nutrient medium for flasks cultivation of the inoculum. A molasses-based nutrient medium was used to incubate the bacilli in a 15-liter fermenter. The finished microbial preparation was obtained in dry form. The biopreparation is a powder consisting of a lyophilically dried concentrates mixture of genus Bacillus spores. Bacilli biomass were pre-mixed with a protective medium based on gelatin and sucrose. The final number of bacteria in the microbial preparation is 1.29(±0.30) ×1012 CFU/g.


2013 ◽  
Vol 45 (2) ◽  
pp. 159-167 ◽  
Author(s):  
Zdeněk PALICE ◽  
Christian PRINTZEN ◽  
Toby SPRIBILLE ◽  
Måns SVENSSON ◽  
Tor TØNSBERG ◽  
...  

AbstractA taxonomic and biogeographic overview of the genusMyrionorais provided. Two species are recognized,M. albidula(Willey) R. C. Harris andM. pseudocyphellariae(Etayo) S. Ekman & Palice comb. nov. The genus is characterized by polysporous asci, the presence of crystals in the hymenium and proper exciple that partly consist of lobaric acid, and a photobiont with large cells (mostly in the range 12–20 µm).Myrionora albidulais currently known from Germany, Norway, Sweden, the Russian Federation (Altayskiy Kray, Chelyabinskaya Oblast', Khabarovskiy Kray and Zabaykal'skiy Kray), and the United States (Alaska, Connecticut, Maine and Massachusetts). It inhabits bark of deciduous trees and shrubs and conifers over a wide range of latitudes.Myrionora pseudocyphellariaeis known from Chile and Ecuador, where it has been encountered on lichens and decaying bark. Based on morphological characteristics, we conclude thatMyrionorabelongs in theRamalinaceae.


Author(s):  
Sergey S. Makarov ◽  
◽  
Galina V. Tyak ◽  
Anton I. Chudetsky ◽  
Irina B. Kuznetsova ◽  
...  

The article presents the results of experimental studies on various propagation methods of the Arctic bramble in the Kostroma region. Industrial cultivation of forest berry plantations is a possible effective solution to the problem of low profitability of using nontimber forest products, reduction of the wild berries resources and their productivity and quality, and reclamation of cutover peatlands. It is advisable to use high-yield varietal planting material to create such plantations. Special attention is paid to propagation and production of healthy planting material using culture of plant cells and tissues. Data on sterilization of explants when introduced in vitro are given. The highest efficiency of sterilization was observed when using a chlorine-free eco-sterilizer (the plant survival rate on the MS nutrient medium was 90–93 %). The effect analysis of the passage number of regenerated plants on the multiplication factor of the Arctic bramble varieties was carried out. The optimal concentrations of cytokinins at the stage of micropropagation are shown. The largest number of the Arctic bramble roots was observed when adding 1.0 mg/L of Indole-3-butyric acid (IBA) and 0.5 mg/L of Ecogel to the nutrient medium. The technological and agrotechnical operations performed during the cultivation of planting material of forest berry plantations are considered. Data on the coefficients of vegetative propagation of plants and their resistance to diseases, yielding capacity, and recultivation of cutover peatlands are given. The best planting material of the Arctic bramble are ball-rooted seedlings. Sawdust and sphagnum were used in the cultivation of this berry on the peatland. The economic efficiency of its cultivation with the method of clonal micropropagation was 358.2 %. For citation: Makarov S.S., Tyak G.V., Kuznetsova I.B., Chudetsky A.I., Tsaregradskaya S.Yu. Producing Planting Material of Rubus arcticus L. by Clonal Micropropagation. Lesnoy Zhurnal [Russian Forestry Journal], 2021, no. 6, pp. 89–99. DOI: 10.37482/0536-1036-2021-6-89-99


2019 ◽  
pp. 3-7 ◽  
Author(s):  
S. A. Vasilchenko ◽  
G. V. Metlina ◽  
N. S. Kravchenko

The nutritional value of soybean seeds is in high protein (up to 35%) and fat (up to 25%) percentage. In the Russian Federation, there is a positive trend in increasing the soybean acreage (e. g. from 1.537 million hectares in 2013 to 2.919 million hectares in 2018). The field trials were carried out in the laboratory of cultivation technology of row crops of the FSBSI “Agricultural Research Center “Donskoy” (Zernograd, southern soil-climatic region of the Rostov region) in 2016–2017. The experiments were conducted with the varieties of local breeding “Don 21” and “Diva”, included in the State Register of Breeding Achievements of the Russian Federation in 6 regions, differing in the length of vegetation period. The soil of the experimental plot is favorable for soybean cultivation, the humus content in the topsoil is 3.36%; pH is 7.0; P2O5 is 24.4; K2O is 360 mg/kg of soil. Depending on the sowing period, the soybean yields varied from 0.85 to 1.25 t/ha for the variety “Don 21” and from 0.77 to 1.38 t/ha for the variety “Diva”. The protein content in soybean seeds was the highest in the fourth term of sowing (41.9 and 39.8%), and the fat content in the first term was 19.0 and 20.2% (the varieties “Don 21” and “Diva” respectively). The analysis of economic and energy efficiency identified the most effective variants of the trial. The variety “Don 21” had the second sowing period, the variety “Diva” had the third one, with the highest values of profitability (129.8 and 147.7%) and the energy efficiency ratio (2.03 and 2.18).


2021 ◽  
Vol 262 ◽  
pp. 01034
Author(s):  
Aniuar Abazov ◽  
Khaset Abidov ◽  
Sultan Basiev ◽  
Khusein Nazranov

Evolution and thousands of years of artificial selection have contributed to the formation of the widest variety of cultivated potatoes. Thanks to this, more than 4 thousand varieties of potatoes have been created in the world today. All of them are known to differ not only in habit, but also in the level of yield, ripening period, resistance degree to numerous diseases and pests, plasticity to certain climatic and soil conditions. The results of research and evaluation of 4 thousand hybrids of the 1st tuber generation (single-tuberous plants) of joint breeding of the Institute of Agriculture ISKh KBNTs RAN and FSBSI (Federal state budgetary scientific institution) “Federal Research Center (FRC) of Potato name after A.G. Lorkh” that passed the full scheme of breeding tests, 9 promising cross-breed populations with economically useful traits were identified: consistently high yield and resistance to a complex of viral, bacterial and fungal diseases. The selected cross-breeds are distinguished by early and medium early ripening periods with a growing season of 55-70 days and a maximum yield of 38.6-66.2 ton/ha, depending on the prevailing weather conditions during the growing season of potato plants in 2019-2020. The genotypes obtained are involved in the implementation of the federal scientific and technical program for the development of potato growing for 2017-2030. The main goal of the program is to create new varieties of early ripeness group of potatoes. The co-executors of this program are a number of research institutes of the Russian Federation, including the Institute of Agriculture ISKh KBNTs RAN. [1] As a result of many years of investigation, the promising cross-breed 2652-15 that emerged from breeding of the Malinovka variety with the backcross 93.20-12 under the name Zamir was transferred in 2020 for the State test of the Russian Federation in the North Caucasian region. Medium early variety for table use and mashed potatoes processing. Potential yield is 49.5 ton/ha. Tubers of an attractive rounded oval shape with shallow eyes weighing 85.5-108.4 g with a yellow color of the tuber and creamy flesh. The tubers are resistant to mechanical damage during combine harvesting, storability is good. Medium resistant to late blight in tops and highly resistant to tubers. Relatively resistant to rhizoctonia and common scab, banded and wrinkled mosaic of leaves.


Author(s):  
S J Bainton ◽  
V E Plumb ◽  
M D Drake ◽  
B O Juliano ◽  
B S Capper

Investigations carried out by the Tropical Development and Research Institute (TDRI) in association with the International Centre for Agricultural Research in Dry Areas (ICARDA), Syria, have shown relationships between the physical characteristics of barley and the nutritive value of its straw. The objectives of the work reported here were to examine rice straw and study the relationships between morphological characteristics (height, harvest index, days to maturity), the proportions of botanical components, chemical constituents and nutritive value of the straw as measured by in vitro cellulase solubility. In addition, environmental effects on some of these characteristics were studied by examining inter-plant variations.


1984 ◽  
Vol 62 (7) ◽  
pp. 1545-1549 ◽  
Author(s):  
E.-C. Pua ◽  
Calvin Chong

Meristem tip explants of Malus robusta Rehd. No. 5 were successfully initiated in vitro on nutrient medium with sorbitol but not with glucose, sucrose, or fructose, each supplied at 30 g/L. Cultures, subsequently subcultured and multiplied on sorbitol medium, produced the highest number of shoots and had the highest shoot fresh and dry weights, followed in descending order by cultures grown on glucose, sucrose, and fructose media. Decreasing the proportion of sorbitol and increasing that of sucrose in the carbon source fraction (30 g/L total) resulted in decreasing shoot number and shoot weight accompanied by increasing leaf/stem ratio and compactness of the shoot cultures. After 32 days on agar medium with 0.5 mg/L indolebutyric acid in the presence of either sorbitol or sucrose, 85% rooting was achieved.


Author(s):  
V. A. Kryukov ◽  
Yu. V. Kryukov

The article deals with modern features of the Arctic economy. It is shown that in those spheres and directions of economic activity, which are associated with the development of natural resources (primarily mineral resources) and focused on obtaining returns on investment, there is a strengthening of the role not only of new knowledge and new technologies, but also significantly increase the role and importance of forms of cooperation of the parties involved in the implementation of projects. This approach allows, on the one hand, to solve the problem of attracting investment in high-risk and, at the same time, high-yield projects, but it also will not allow to fully realize the opportunities associated with the development and use of domestic research and production potential in the implementation of projects in the framework of such “hybrid forms”. One of the direct and immediate consequences of this approach in the practice of field development in the Arctic zone of the Russian Federation (AZRF) is a significant increase in the knowledge-intensive service sector, which meets the needs for equipment and labor by borrowing foreign advanced technologies and the widespread use of interregional watch. These processes lead, in particular, to the fragmentation of the economic space of the country (reducing the degree of connectivity of the economies of different regions), as well as to the stagnation and extinction of urbanized settlements in the Russian Federation.


2020 ◽  
pp. 41-45
Author(s):  
S. G. Golovko ◽  
N. V. Kalinina ◽  
A. A. Yatsyna ◽  
N. N. Vozhzhova ◽  
E. V. Ionova

The improvement of genetic diversity and acceleration of breeding process are the most important tasks in wheat breeding. In vitro biotechnological methods are of practical interest for breeding process. The current paper has considered the effects of genotypes and nutritious medium on the formation of regenerants. The purpose of the research was to study the ability to androgenesis in winter soft wheat anthers and to identify promising winter soft wheat genotypes. The estimation of the ability to androgenesis was carried out among ten winter wheat samples. 8711 anthers were planted on medium of N6 and PII induction; the 190-2 medium was used for regeneration. As a result of the study, it was found that the process of neoplasms occurrence depends both on the variant of the nutrient medium and on the chosen genotype. It has been proven that the most favorable medium for the cultivation of winter soft wheat anthers is medium N6. It was found that the maximum percentage of neoplasms (5.21%) from winter soft wheat anthers was recorded in the sample “595/13”. It was found that the samples “595/13” (9 plants) and “Niva Dona” (6 plants) developed by the FSBSI “Agricultural Research Center “Donskoy” showed the highest responsiveness to the formation of calluses and plants-regenerants. There were obtained 6 green regenerants and 3 albino forms from the pollen callus of the sample “595/13” and 4 green regenerants and 2 albino regenerants from the sample “Niva Dona”.


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