scholarly journals Peculiarities of change in number and composition of winter wheat rhizosphere microbiome in the process of ontogenesis

2021 ◽  
Vol 12 (3) ◽  
pp. 56-65
Author(s):  
A. N. Honchar ◽  
◽  
O. L. Tonkha ◽  
N. V. Patyka ◽  
O. S. Makarchuk ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Soil Microorganisms ◽  
Soil Formation ◽  
Environmental Safety ◽  
Soil Samples ◽  
Typical Chernozem ◽  
Wheat Rhizosphere ◽  
Process Plant ◽  
Microbial Complex ◽  
Manometric Method

In agroecosystems, microorganisms are the main factor in the soil formation process, plant nutrition and phytosanitary condition of the soil. Therefore, all measures aimed at restoring soil fertility and increasing productivity, environmental safety of agricultural production are closely linked to the activities of microorganisms. Increased local activity, biomass and diversity of microbiota are one of the most important characteristics that distinguish the rhizosphere from the total soil volume. The aim of the research was to study the number and composition of the microbial complex of the rhizosphere of cereals (winter wheat of different varieties of domestic selection) in the process of ontogenesis. To conduct a set of microbiological studies, soil samples were taken and prepared (typical chernozem) in compliance with the standard requirements for sample preparation and storage of samples in the laboratory. The analysis of rhizosphere soil samples was performed according to the variants of different varieties of winter wheat of domestic selection. The number of soil microorganisms was determined by the method of inoculation of suspensions on nutrient media according to generally accepted methods in microbiology. Determination of the total microbial biomass in the soil was carried out by the rehydration method, and the intensity of "respiration" of the soil, respectively, by the release of CO2 and O2 absorption by the manometric method (Warburg apparatus). It is established that varietal specificity is significantly related to the peculiarities of microbiome formation in different phases of plant growth and development, which is an integral indicator of functional and metabolic activity of soil microorganisms. The number and composition of the microbial complex of winter wheat rhizosphere in the process of ontogenesis changes significantly, especially in the ratio of the number of spore-forming and non-spore forms of microorganisms under the same conditions of crop cultivation. The total pool of saprotrophic microorganisms of the rhizosphere demonstrates the variability of biomass and changes in favor of ecoplastic Bacillus. An increase in the number of spore-forming bacteria to 4.2 x 107 CFU/g in the variants of growing certain varieties was found. It is shown that in the cultivation of different varieties of winter wheat there are stable indicators of the intensity of CO2 emissions - from 5.2 to 7.0. A similar trend can be traced to the absorption of O2 (not more than 5.3-6.8).

Soil response to chemicals used in a field experiment

2013 ◽  
Vol 27 (2) ◽  
pp. 151-158 ◽  
Author(s):  
S. Jezierska-Tys ◽  
A. Rutkowska
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Field Experiment ◽  
Soil Microorganisms ◽  
Block Design ◽  
Soil Samples ◽  
Alkaline Phosphatases ◽  
Soil Response ◽  
Negative Effect ◽  
Set Up

Abstract The effect of chemicals (Reglone 200 SL and Elastiq 550 EC) on soil microorganisms and their enzymatic activity was estimated. The study was conducted in a field experiment which was set up in the split-block design and comprised three treatments. Soil samples were taken six times, twice in each year of study. The results showed that the application of chemicals generally had no negative effect on the number of soil microorganisms. The application of Reglone 200 SL caused an increase of proteolytic and ureolytic activity and affected the activity of dehydrogenases, acid and alkaline phosphatases in the soil. The soil subjected of Elastiq 550 EC was characterized by lower activity of dehydrogenases, protease, urease and alkaline phosphatase.

scholarly journals Cyber-Physical Industrial Control System Testbed: A Cyber-Security Solution

2020 ◽  
Author(s):  
Mohammad Nourizadeh ◽  
Mohammad Shakerpour ◽  
Nader Meskin ◽  
Devrim Unal
Keyword(s):  
Cyber Security ◽  
Process Model ◽  
Degrees Of Freedom ◽  
Industrial Process ◽  
Environmental Safety ◽  
Open Loop ◽  
Industrial Control ◽  
Systems Research ◽  
Process Plant ◽  
Real Risk

In this project, the hybrid testbed architecture is selected for the development of ICS testbed where the Tennessee Eastman (TE) plant is simulated inside PC and the remaining components are implemented using real industrial hardware. TE plant is selected as the industrial process for the developed cybersecur ity testbed due to the following reasons. First, the TE modTheel is a wellknown chemical process plant used in control systems research and it dynamics is well understood. Second, it should be properly cont rolled otherwise small disturbance will drive the system toward an unsafe and unstable operat ion. The inherent unstable open-loop property of the TE process model presents a real-world scenario in which a cyberattack could represent a real risk to human safety, environmental safety, and economic viability. Third, the process is complex, coupled and nonlinear, and has many degrees of freedom by which to control and perturb the dynamics of the process.

scholarly journals The significance of soil microorganisms as a limiting factor in infection of clover by Sclerotinia trifoliorum erikss. at different times of the year

1964 ◽  
Vol 36 (1) ◽  
pp. 120-134
Author(s):  
Anna-Marja Halkilahti
Keyword(s):  
Soil Microorganisms ◽  
Soil Microbes ◽  
Marked Decrease ◽  
Petri Dish ◽  
Soil Samples ◽  
Pronounced Effect ◽  
The Other ◽  
Limiting Factor ◽  
Sclerotinia Trifoliorum ◽  
Other Hand

In the petri dish trials the mycelia of Sclerotinia trifoliorum in sterilized soil samples generally infected clover quite readily. In unsterilized samples the infection was less severe and showed marked variations in different years and at different times of the year. In the outdoor trials clover became less infected in the middle of the summer than in the spring and autumn. On the other hand, in the thermostat trials, where the temperature was constantly 7—10°C, the temperature at the time of taking the soil samples did not have a pronounced effect on the extent of clover infection. Liming of the soil caused a marked decrease in the severity of clover rot infection. In some autumns clover plants growing in soil samples taken from fallow were more seriously infected than those growing in soil from a clover field. As a rule, however, there were only slight variations in the extent of infection in the soil samples taken at the same time from the various areas of the field cultivated in different ways. In general, the highest numbers of microorganisms in the soil were found in the autumn and the lowest in the spring. In clover fields there were often more microorganisms – particularly in the autumn – than in fallow. Liming caused an increase in the numbers of soil microbes. S. trifoliorum infected clover very severely at temperatures of 0—5°C; in the range 5—21°C the infection grew generally milder the more the temperature was found to be rising. At temperatures of 5– 10°C an increase in the numbers of soil microbes resulted in a decrease in the infection of the clover. Still higher temperatures, which did not increase the numbers of soil microorganisms, nevertheless enhanced the antagonistic power of the soil.

scholarly journals The chemicals (formaldehyde and toluene) influence on soil microorganisms of leached chernozem

2016 ◽  
Vol 5 (3) ◽  
pp. 21-25
Author(s):  
Natalya Anatolyevna Ilyina ◽  
Tatyana Valentinovna Fufaeva ◽  
Natalya Anatolyevna Kazakova ◽  
Nataliya Mikhailovna Kasatkina ◽  
Evgeniya Alexandrovna Vilkova
Keyword(s):  
Soil Microorganisms ◽  
Heterotrophic Bacteria ◽  
Soil Formation ◽  
Chemical Production ◽  
Leached Chernozem ◽  
Physico Chemical ◽  
Production And Consumption ◽  
The Status ◽  
Number Of Microorganisms ◽  
Bacteria And Fungi

The paper assesses the status of the soil cover associated with the pollution of its waste chemical production and consumption. The authors present the data of formaldehyde and toluene influence on the abundance of actinomycetes, heterotrophic bacteria and fungi, as these groups of microorganisms provide self-purification capacity of the soil and participate in soil formation processes. In this paper microbiological and physico-chemical methods of research are used. The study of species composition changes of some soil microorganisms groups of leached chernozem under the influence of formaldehyde and toluene showed that this factor causes changes in the complex of soil microorganisms. This factor is reflected in the decreased species richness and diversity and increase of pollution-tolerant microorganisms. These studies investigate a number of microorganisms that provide self-purification capacity of the soil and participate in soil formation. The results show the nature of the influence of different doses of formaldehyde and toluene on the structure and functioning of the complex of soil microorganisms, as well as reveal the mechanism of action of chemicals (formaldehyde and toluene) on soil microbiota associated with its resistance and the manifestation of toxicity of the soil.

scholarly journals Effectiveness of winter wheat cultivation in adaptive crop rotation on chernozem typical

2021 ◽  
Vol 32 ◽  
pp. 01007
Author(s):  
Ivan Prushchik ◽  
Svetlana Khlupina
Keyword(s):  
Winter Wheat ◽  
Crop Rotation ◽  
Maximum Yield ◽  
Atmospheric Precipitation ◽  
Weather Conditions ◽  
Farming System ◽  
Crop Rotations ◽  
Net Income ◽  
Typical Chernozem ◽  
Wheat Cultivation

The paper presents the results of research to assess the efficiency of winter wheat (Triticum aestivum L.) cultivation in adaptive crop rotations on typical chernozem. The crop yield have analyzed for three different crop rotations (grain-fallow, grain-grass-row, and grain-grass) in comparison with a monoculture on a stationary multifactorial field experiment on physical modeling of the farming system of the FSBSI “Kursk FARC” (Kursk region, Medvensky district). It was determined that the maximum yield was obtained in grain-and-row crop rotation; on average, over three years of research, the increase in it was 1.71 t/ha. Statistical data processing has carried out and correlations between the weather conditions of the year and the yield of winter wheat has revealed. Thus, a moderate direct relationship (r = 0.65) was established with atmospheric precipitation, and a moderate inverse relationship with the sum of effective temperatures (r = 0.58). Indicators of economic efficiency of winter wheat cultivation have calculated, both for monoculture and for three types of crop rotations. The lowest cost of winter wheat grain – 5926.94 t/ha – was recorded in grain-fallow crop rotation, which provided the highest net income of 12056.26 and the highest profitability among all options – 68.72%.

DYNAMICS OF HUMUS RESERVES AND FORECAST OF CARBON-INVESTING POTENTIAL OF ZONAL SOILS OF THE REPUBLIC OF TATARSTAN

2021 ◽  
Vol 16 (3) ◽  
pp. 27-31
Author(s):  
Liliya Gaffarova
Keyword(s):  
Forest Soil ◽  
Large Scale ◽  
Gray Forest Soil ◽  
Humus Content ◽  
Statistical Processing ◽  
Soil Formation ◽  
Typical Chernozem ◽  
The Republic ◽  
Soil Formation Factors ◽  
Zonal Soils

The article analyzes the results of statistical processing of morphometric and agrochemical indicators of zonal soils of the Republic of Tatarstan based on the accumulated information of large-scale soil studies. The boundaries of genetic horizons are examined, the content of humus and its reserves within the limits of heavy loamy and clay varieties of arable soil subtypes is estimated. Arithmetic averages allow us to generalize the humus content in soils. So, in the series of sod-podzolic soils – light gray forest-gray forest – dark gray forest-podzolic chernozems – leached chernozems-typical soils in a layer of 0-30 cm on average contains 2,20; 2,58; 3,76; 4,76; 6,8; 7,6; 7,2 % humus. The observed patterns of humus accumulation in soils are also preserved in the distribution of its reserves – 85; 97; 140; 162; 233; 257; 240 tons per he-1. It is proposed to characterize the general population along the boundaries of typical values that fit into the 50% confidence interval to assess the overall range of spatial variability of humus indicators. The upper limit of the typicity will correspond to the greatest accumulation of humus with a combination of local features of soil formation factors and can serve as a guide for predicting carbon sequestration in the soils of this region. Accordingly, the carbon-sequestering potential of sod-podzolic soil and typical chernozem is-20 tons per he-1, light gray forest soil-22 tons per he-1, gray forest soil and leached chernozem-27 tons per he-1, the highest values in dark gray forest soil are more than 30 tons per he-1

Structural and spatial organization of the microbial biom of the rhiosphere of plants of eternal green such (Buxus sempervirens L.)

2020 ◽  
pp. 244-250
Author(s):  
S.V. Veretiuk ◽  
◽  
T.I. Patyka ◽  
N.V. Patyka ◽  
M.V. Boiko ◽  
...  
Keyword(s):  
Soil Microorganisms ◽  
Spatial Organization ◽  
Physiological State ◽  
Morphological Properties ◽  
Rhizosphere Microorganisms ◽  
Microbial Complex ◽  
Buxus Sempervirens ◽  
Taxonomic Groups ◽  
Quantitative Indicators ◽  
Number Of Bacteria

The features of the formation of the structural and spatial organization and biodiversity of the microbial complex of the rhizosphere of plants of the evergreen boxwood (Búxus sempervírens L.) in closed ground are considered. The abundance of the main taxonomic groups of microorganisms of the evergreen boxwood rhizosphere: bacteria, micromycetes, and actinomycetes has been estimated. According to the soil microorganisms population structure results, was established that the quantitative indicators of boxwood rhizospheric microorganisms depends on the physiological state and age of the plants. It has been investigated that the rhizosphere of boxwood is characterized by an insignificant variety of bacterial and fungal microflora. It was shown that the number of bacteria in plant ontogenesis increases from 2.09 ± 0.37 to 4.90 ± 0.62 million/g of soil (10-year-old plants). There is a dynamics of an increase in the number of micromycetes by more than 10 times (2.16 ± 1.22 – 2 year olds; 20.24 ± 2.55 – 4 year olds; 25.94 ± 3.24 thousand CFU/g – 10 year olds) and actinomycetes – 2.4 times (1.87 ± 0.03 million CFU/g). When analyzing the qualitative composition and cultural and morphological properties of isolates of rhizosphere microorganisms of evergreen boxwood, dominant forms were identified that are assigned to representatives of the genera Bacillus, Pseudomonas, Rhizopus and Trichoderma.

scholarly journals Turnover of Minerals and Organics in the Postharvest Herbage of Annuals and Perennials: Winter Wheat and Goldenrod

Agriculture ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 170 ◽  
Author(s):  
Gerhard Gramss ◽  
Klaus-Dieter Voigt
Keyword(s):  
Winter Wheat ◽  
Lignin Content ◽  
Root Exudation ◽  
Soil Formation ◽  
Dry Weight ◽  
Water Soluble ◽  
Co2 Efflux ◽  
Transient Rise ◽  
Biomass N ◽  
Microbial Carbon

Crossing annual cereals, legumes, and oilseeds with wild rhizomatous relatives is used to create perennial lines that fruit over 2–3 seasons. Contrary to annual crops, the year-round vegetation cover should contribute to carbon sequestration, soil formation, and root mineral preservation. Soil erosion, nutrient leaching, and labor expenses may be reduced. While deep-rooted grasses actually inhibit nitrate leaching, advantages in nutrient storage and soil formation are not yet shown. Therefore, the turnover of organics and minerals in the perennial goldenrod was compared with that of winter wheat between blooming and resprouting (28 February) by gravimetry and ICP-MS. From blooming (23 August) to harvest (13 November), goldenrod stalks of 10,070 (given in kg ha−1) lost 23% by dry weight (DW) and released 14.9/9.6/65.7 in NPK and 2193 in water-soluble organics via leaching and root exudation. Apart from a transient rise of 28.8 in N around 13 November, the stubble/rhizome system held CaKMg(N)P stable at a level avoiding metal stress from 23 August to 28 February. Filling seeds in wheat excluded net losses of minerals and organics from anthesis to harvest (23 July). Stubbles (16 cm) and spilt grains of 2890 represented 41.8/2.91/62.5 in NPK and lost 905 in biomass with 25.4/1.8/59.8 in NPK to the soil by 28 February. In wheat-maize rotations, ploughing was avoided until early March. Weeds and seedlings emerged from spilt grains replaced losses in stubble biomass, N, and P but left 40.5 in K unused to the soil. In wheat-wheat rotations, organics and minerals lost by the down-ploughed biomass were replenished by the next-rotation seedlings that left only 18.3 in K to the soil. In summary, off-season goldenrod rhizomes did not store excess minerals. The rate of mineral preservation corresponded with the quantity of the biomass irrespective of its perennial habit. Released water-soluble organics should foster microbial carbon formation and CO2 efflux while soil improving gains in humate C should depend on the lignin content of the decaying annual or perennial biomass. Clues for NPK savings by perennials were not found.

scholarly journals Communities of bacteria and fungi occurring in the rhizosphere of winter wheat

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 361-363
Author(s):  
E. Patkowska ◽  
D. Pięta ◽  
A. Pastucha
Keyword(s):  
Species Composition ◽  
Winter Wheat ◽  
Pathogenic Fungi ◽  
Microbiological Analysis ◽  
Qualitative Composition ◽  
Fusarium Spp ◽  
Wheat Rhizosphere ◽  
Bacillus Spp ◽  
Bacteria And Fungi ◽  
Total Bacteria

The purpose of the present studies was to establish the species composition of fungi occurring on the underground parts of winter wheat, Kobra cv. and to determine the quantitative and qualitative composition of microorganisms developing in the rhizosphere of this plant. The mycological analysis of the infected roots and the stem base of winter wheat gave 320 fungi isolates. Fusarium spp. were most frequently isolated, and their proportion was 64.7% of all the fungi. This genus was represented by F. avenaceum, F. culmorum, F. equiseti, F. graminearum, F. oxysporum and F. solani. Among these species the dominating ones were F. avenaceum (19.4%) and F. culmorum (38.7%). The microbiological analysis of winter wheat rhizosphere gave the highest number of total bacteria (6.32 × 106 cfu/g of d.w. of soil). The number of Pseudomonas pp. was 3.56 × 106 cfu/g of d.w. of soil, and the number of Bacillus spp. was 2.42 × 106 cfu/g of d.w. of soil. Fusarium spp. and Rhizoctonia solani dominated within pathogenic fungi isolated from the rhizosphere of winter wheat.

scholarly journals Profiling the Bacterial Diversity in a Typical Karst Tiankeng of China

Biomolecules ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 187 ◽  
Author(s):  
Gaozhong Pu ◽  
Yanna Lv ◽  
Lina Dong ◽  
Longwu Zhou ◽  
Kechao Huang ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Communities ◽  
Soil Microorganisms ◽  
Operational Taxonomic Unit ◽  
Soil Characteristics ◽  
Soil Samples ◽  
Controlling Factors ◽  
Bacterial Composition ◽  
Microbial Distribution ◽  
Significant Difference

While karst tiankengs have a higher capacity to act as safe havens for biodiversity in changing climates, little is known about their soil microorganisms. To fill this gap, we investigate the distribution and driving factors of the bacterial community in karst tiankeng systems. There is a significant difference in the soil characteristics between the inside and the outside of a karst tiankeng. At the karst tiankeng considered in this study, the bacterial composition, in terms of the operational taxonomic unit (OTU), was found to be significantly different in different soil samples, taken from diverse sampling sites within the collapsed doline or the external area, and showed a high habitat heterogeneity. The dominant phylum abundances vary with the sampling sites and have their own indicator taxa from phylum to genus. Unlike the primary controlling factors of plant diversity, the microclimate (soil moisture and temperature), soil pH, and slope dominated the distribution of the bacterial community in karst tiankeng systems. Our results firstly showed the distribution characteristics of bacterial communities and then revealed the importance of microhabitats in predicting the microbial distribution in karst tiankeng systems.

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