scholarly journals Hematological and biochemical indicators of rainbown trout blood depending on lysin and methionine levels in feed

2021 ◽  
Author(s):  
V. М. Kondratiuk ◽  
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Control Group ◽  
Biochemical Indicators ◽  
Group 5 ◽  
Combined Feed ◽  
Limiting Amino Acids ◽  
Experimental Group ◽  
Different Levels

The effect of use of complete feed with different lysine and methionine levels on the hematological and biochemical indicators of rainbow trout blood is studied in the article. The aim of the experiment was to establish the effect of different levels of amino acid nutrition of commercial rainbow trout on hematological and biochemical indicators of its blood. For this purpose, five experimental groups were formed by the analog method. The study lasted 210 days and was divided into two periods: equalizing (10 days) and basic (200 days). In the equalizing period, the trial fish consumed feed of the control group. In the basic period of the experiment, the level of lysine and methionine in the production feed ranged from 2.5 to 2.9% and from 0.8 to 1.0%, respectively. It was found that the different content of lysine and methionine in the diets of two-year-old rainbow trout did not cause significant changes in biochemical indicators of blood. The increase in the content of limiting amino acids in the combined feed of young rainbow trout caused slight deviations in hemoglobin content compared to control. The analysis of the obtained results revealed the highest content of erythrocytes (1.28 T/l) in the blood of specimen of experimental group 5, the level of amino acids in combined feeds was the lowest. Its highest content was found in the blood plasma of trout of experimental group 4, which received combined feed with a content of 2.8 % lysine and 0.95 % methionine. The concentration of total protein in the blood serum was almost the same in all experimental groups of commercial rainbow trout. It was found that different levels of amino acid nutrition of rainbow trout did not affect the dynamics of changes in the blood cell morphology differentials.

scholarly journals THE INFLUENCE OF AMINO ACID NUTRITION ON THE PRODUCTIVITY OF RAINBOW TROUT

2020 ◽  
pp. 104-114
Author(s):  
Vadym Kondratiuk
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Unit Weight ◽  
Control Group ◽  
Economic Optimization ◽  
Experimental Fish ◽  
Growth Intensity ◽  
High Amino Acid ◽  
Different Levels

The article considers the effectiveness of complete feed with different levels of lysine and methionine for growing rainbow trout up to 10 g. The experiment aimed to establish the effect of different levels of amino acid nutrition of trout on its productivity. For this purpose, five experimental groups of this year were formed by the method of analogues. The experiment lasted 45 days and was divided into two periods: equalization (5 days) and main (40 days). During the equalization period, the experimental fish consumed feed of the control group. In the main period, the level of lysine and methionine in experimental feeds for different experimental groups of trout ranged from 2.8 to 3.2 % and from 0.90 to 1.10 %, respectively. As a result of research, it was found that the use of trout weighing up to 10 g of feed with high amino acid nutrition (3.1 % lysine, 1.05 % methionine) in the feeding of this year is accompanied by an increase in their weight by 9.2 % (p <0.05) and growth intensity - by 4.1-9.3 %, compared with analogues who consumed feed with the content of lysine of 3.0% and methionine of 1.0%. The reduction of these amino acids to the levels of 2.8 and 0.9 %, respectively, contributes to a probable decrease (p <0.01) in the weight of fish by 14.1 %, and a decrease in growth intensity by 8.7-17.3 %. It was proved that different levels of amino acid nutrition of young trout significantly (p <0.01) affected the weight of experimental fish. The share of influence of this factor is 61.4 %, which is 22.8 % more than the influence of other factors. In the course of research, it was determined that when growing rainbow trout this year up to 10 g, feeding them compound feeds with lysine and methionine content of 3.1 and 1.05% helps to reduce feed costs per unit weight gain by 12.5 %, compared with using feeds with levels of these amino acids of 2.8 and 0.9 %, respectively. At the same time, the safety of the experimental fish throughout the experiment corresponded to the normative indicators in trout farming and was in the range of 76.0-77.3 %. The analysis of the obtained fish farming results showed that for the production of trout products according to the indicators of maximum productivity and economic optimization criteria it is recommended to use complete feed with a level of 3.1% lysine and 1.05% methionine for feeding rainbow trout.

scholarly journals Rearing larvae and juveniles of rainbow trout with different amino acid nutrition

2020 ◽  
Author(s):  
V. Kondratiuk ◽  
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Juvenile Fish ◽  
Control Group ◽  
Economic Optimization ◽  
Larvae And Juveniles ◽  
Experimental Fish ◽  
Juvenile Trout ◽  
High Level

The article considers the efficiency of using complete compound feeds with different levels of lysine and methionine in rearing larvae and juveniles of rainbow trout. The purpose of the experiment was to establish the effect of different amino acid levels in nutrition of juvenile trout on its productivity. For this purpose, five experimental groups were formed by the method of analogues. The experiment lasted 35 days and was divided into two periods: equalizing (5 days) and main (30 days). During the equalizing period, the experimental fish consumed feed of the control group. In the main period, the levels of lysine and methionine in experimental feeds for different experimental groups of trout ranged from 2.9 to 3.3% and from 0.95 to 3.15%, respectively. It is proved that increasing the content of lysine and methionine in feed for larvae and juveniles of rainbow trout to the levels of 3.2 and 1.1%, respectively, promotes increase in their weight by 10.7% (p <0.01) and growth intensity - by 6.4-11.1%, while further increase in these amino acids content in the feed reduces the productivity of fish. Polynomial equations for determining the juvenile fish weight at any stage of rearing at a high level of determination for each of the experimental groups have been calculated. It was found that with an increase in the content of lysine and methionine in feed to the levels of 3.2 and 1.1%, respectively, reduces feed costs per 1 kg of weight gain in trout larvae and juveniles by 4.1%, and a further increase in levels of these amino acids to 3.3 and 1.15%, respectively, increase this index by 1.1%. The experimental fish survival ranged from 83.9 to 85.1%. In the production of trout according to the criteria of maximum productivity and to the economic optimization criteria, it is recommended to use complete feed with the level of lysine - 3.2% and methionine - 1.1% for feeding trout larvae and juveniles.

scholarly journals The effect of dietary amino acid abundance and isotopic composition on the growth rate, metabolism and tissue δ13C of rainbow trout

2011 ◽  
Vol 105 (12) ◽  
pp. 1764-1771 ◽  
Author(s):  
Julia Gaye-Siessegger ◽  
James S. O. McCullagh ◽  
Ulfert Focken
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Essential Amino Acid ◽  
Control Group ◽  
Body Tissues ◽  
Synthetic Amino Acids ◽  
Dietary Amino Acid

The aim of the present study was to test whether the dietary non-essential/conditionally essential amino acid composition has an effect on growth and protein utilisation and on δ13C of individual amino acids in rainbow trout (Oncorhynchus mykiss). Trout were reared on six purified diets containing only synthetic amino acids in place of protein. Diet 1 mimicked the amino acid composition of fishmeal, in diet 2, cysteine (Cys), glycine (Gly), proline (Pro) and tyrosine (Tyr) were isonitrogenously replaced by their precursor amino acids serine (Ser), glutamic acid (Glu) and phenylalanine (Phe), and in diet 3, alanine (Ala), asparagine and aspartate, Cys, Gly, Pro, Ser and Tyr were isonitrogenously replaced by Glu. Diets 4, 5 and 6 resembled diets 1, 2 and 3 except that Glu contained 0·1 % 13C-enriched Glu. A control group was reared on a fishmeal-based diet. A total of forty-two trout (4·7 (sd 0·57) g) were fed one of the diets at a level of 3·5 % body mass for 10 weeks in a flow-through system. Dietary non-essential amino acid composition significantly influenced protein gain (P < 0·025) and δ13C of Ala, arginine (Arg), Gly, histidine (His), Phe and Tyr. Non-enriched Glu was predominantly found in trout fed 13C-enriched Glu, which is consistent with the fact that Glu has been shown to be used extensively in the gut as an energy source but is less consistent with the enrichment of Pro in fish fed diet 6 compared with fish fed diet 3. Further research is required to better understand the mechanisms that lead to the alteration of amino acid δ13C between diet and body tissues.

scholarly journals Теоретичне обгрунтування співвідношення треоніну і триптофану у раціонах ремонтних курчат

2017 ◽  
Vol 7 (3) ◽  
pp. 111-115
Author(s):  
M.J. Krivenok ◽  
I.I. Ilchuk ◽  
V.M. Mykhalska
Keyword(s):  
Mathematical Model ◽  
Amino Acids ◽  
Amino Acid ◽  
Nonlinear Model ◽  
Linear Dependence ◽  
Growth And Development ◽  
Control Group ◽  
Growing Period ◽  
Different Content ◽  
Different Levels

<p>We determined the optimal content and ratio of threonine and tryptophan in the mix fodder of replacement chicks at different periods of their growing. We studied the effect of different levels of threonine and tryptophan in mixed fodder on chicken growth and development. We registered that under optimal AA content and ratio the replacement chickens at the end of the growing period outweighed the chickens from control group by 140 g. We also studied the influence of different content of AA and temporal changes in their ratio on digestibility of feed nutrients by replacement chickens from market egg flock. It was found that the optimal content of threonine and tryptophan and their ratio at certain periods of chicken growth caused the increase in digestibility of protein by 2 %, fiber – by 1 %, and BEV – by 2 %. We also proved that the decrease or increase of AA ratio in mix fodder of replacement chickens leads to the manifestation of antagonism between amino acids and the deterioration of their function in bird organism. The changes in AA ratio had significant effects on chicken productivity, the digestibility of feed nutrients, the assimilation, and efficiency of amino acid use. This effects were considerably stronger than the effect of changes in AA content. The degree of influence of the ratio between threonine and tryptophan varies throughout the growing period, it has been established that the ratio of threonine to tryptophan in the period of limited chick feeding (9-6 weeks) has a lesser effect on the productivity of repair young (R<sup>2</sup> = 0.89) the second one (R<sup>2</sup> = 0.92) and the last growing periods (R<sup>2</sup> = 0.92). We performed a mathematical model (polynomial trend line) to describe the growth of replacement chickens during all experiment periods, the pattern of dependences between chicken organism and AA content, AA ratio, age, and productivity. This nonlinear model is more relevant and reliable than linear dependence.</p>

Investigation of dependences of the morphological composition of body and amino acid composition of trout meat proteins (Oncorhynchus mykiss) on levels of the energy value of feeds

10.5219/1601 ◽  
2021 ◽  
Vol 15 ◽  
pp. 497-505
Author(s):  
Vadym Kondratiuk ◽  
Volodymyr Otchenashko
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
The Body ◽  
Metabolic Energy ◽  
Control Group ◽  
Morphological Composition ◽  
Meat Proteins

The article studies the effect of using complete compound feeds with different energy levels on the morphological composition of the body and the amino acid composition of trout meat proteins. The experiment aimed to establish the influence of different levels of energy nutrition of commercial rainbow trout on the morphological composition of their body and the amino acid composition of meat proteins. For this purpose, five experimental groups were formed using the analog method. The study lasted 210 days and was divided into two periods: comparative (10 days) and main (200 days). During the comparative period, the study fish consumed compound feed of the control group. During the main period, the energy level in experimental compound feeds for different experimental trout groups ranged from 16 to 20 mJ per 1 kg. It was found that with an increase in the mass of two-year-old trout, the mass of muscle tissue and the yield of edible parts probably increases. Feeding fish with an increased amount of metabolic energy (20 mJ.kg-1) resulted in a significant change in the weight of internal organs, including the heart, liver, and kidneys. Increasing the metabolic energy in the compound feeds of fish from 18 mJ.kg-1 to 19 – 20 mJ.kg-1 leads to a significant increase in bowel mass by 13.3 – 5.0%. An increase in the level of metabolic energy in rainbow trout diets from 18 mJ.kg-1 to 19 – 20 mJ.kg-1 contributed to a likely increase in the methionine content in meat. A similar pattern was observed for the tryptophan content. It was found that the content of most essential amino acids in the protein of rainbow trout meat exceeds the corresponding values in the "ideal" protein, except for the content of isoleucine and leucine, which refers them to limiting amino acids.

scholarly journals The efficiency of growing rainbow trout (Oncorhynchus mykiss) at different levels of lysine and methionine in feeding

2021 ◽  
pp. 173-181
Author(s):  
V. Kondratiuk
Keyword(s):  
Rainbow Trout ◽  
Amino Acid ◽  
Experimental Studies ◽  
Feed Consumption ◽  
Control Group ◽  
Positive Trend ◽  
Economic Optimization ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Significant Difference ◽  
Different Levels

The article considers the issue of the effectiveness of the use of complete feed with different levels of lysine and methionine for the cultivation of rainbow trout. Experimental studies on this yearling rainbow trout є (Walbaum, 1792) were conducted in the farm «Shipot» Perechyn district of Transcarpathian region. The aim of the experiment was to establish the effect of different levels of amino acid nutrition of this yearling trout on its performance. For this purpose, five experimental groups were formed by the method of analogues. The experiment lasted 55 days and was divided into two periods: equalization (5 days) and basic (50 days). During the equalization period, the experimental fish consumed feed of the control group. In the basic period, the level of lysine and methionine in experimental feeds for different experimental groups of trout ranged from 2.7 to 3.1% and from 0.85 to 1.05%, respectively. Trout were fed 6 times a day during the growing season. The required amount of feed was calculated according to the indicators of individual weight of young and the temperature of the environment at the time of feeding. Trout were grown in ponds at a water level of 1 m. Weighing of experimental young trout was performed once every 5 days. It is proved that the reduction of lysine content to 2.7% and methionine to 0.85% in compound feed for this year trout weighing more than 10 g causes a significant (p<0.05) decrease in fish productivity. In particular, it was found that feeding compound feeds with the above amino acid levels helps to reduce trout weight by 10.4% and reduce growth rate by 9.1–19.9% compared to fish fed lysine and methionine at level 2.9 and 0.95%, respectively. The increase in these indicators in the feed for trout this year is accompanied by some positive trend to increase their productivity, but no significant difference has been established. In the course of research it was determined that the feed consumption per 1 kg of weight gain in this year of trout, which received feed with a content of lysine 3.0% and methionine 1.0% were lower by 10.3%, and with the content of these amino acids at level 2.7 and 0.85%, respectively — 17.1% higher compared to fish that consumed food with a content of lysine 2.9 and methionine 0.95%. The use of complete feed with different levels of lysine and methionine for feeding trout this year did not significantly affect the safety of fish, which was in the range of 82.0–83.1%. For the production of trout products in terms of maximum productivity and economic optimization criteria, for feeding trout weighing more than 10 g, it is recommended to use complete feed with a level of lysine — 3.0% and methionine — 1.0%.

scholarly journals Influence of macro and microelements on the biological value of wheat grain

2021 ◽  
Vol 937 (2) ◽  
pp. 022130
Author(s):  
V Isaychev ◽  
N Andreev ◽  
F Mudarisov
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Spring Wheat ◽  
Protein Quality ◽  
Mineral Fertilizers ◽  
Control Group ◽  
Natural Fertility ◽  
Biological Value ◽  
Amino Acid Score ◽  
Limiting Amino Acids

Abstract This paper considers the influence of manganese and zinc sulfate, as well as Megamix-Zinc on the protein quality of grain of winter and spring wheat, when cultivating it on soils with a deficiency of certain microelements. It was found that treatment of seeds and growing plants of experimental crops with the studied preparations increased the content of the essential limiting amino acids in the grain of winter wheat - lysine by 85.7%, threonine by 116.7%, isoleucine + leucine by 20.9%, phenylalanine + tyrosine by 33.3% compared to the control variant. A rise in the amount and amino acid score of such limiting amino acids as lysine and threonine is also observed, which leads to a decline in the coefficient of difference of amino-acid scores up to 75.5% and an increase in the biological value of the total protein up to 24.5%. The total amount of amino acids in the grain of spring wheat increased when Megamix-Zinc was used in the cultivation technology, both on soils with natural fertility and with application of mineral fertilizers, which is associated with a rise in nitrogen and carbohydrate metabolism. The increase in relation to the control group is 0.42 - 1.34 mg/g, depending on the variant. Under the influence of the factors used, amino acid score of individual amino acids of the spring wheat protein increases in relation to the control group up to 2.1%.

Plasma-Free Amino Acid Profiling of Nasal Polyposis Patients

2020 ◽  
Vol 22 (9) ◽  
pp. 657-662 ◽  
Author(s):  
Mustafa Celik ◽  
Alper Şen ◽  
İsmail Koyuncu ◽  
Ataman Gönel
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Nasal Polyposis ◽  
Amino Acid Profile ◽  
Healthy Control Group ◽  
Control Group ◽  
Healthy Control ◽  
History Of

Aim and Objective:: To determine the mechanisms present in the etiopathogenesis of nasal polyposis. It is not clear whether amino acids contribute in a causal way to the development of the disease. Therefore, the aim of this study was to determine the plasma-free amino acid profile in patients with nasal polyposis and to compare the results with a healthy control group. Materials and Methods:: This was a prospective controlled study that took place in the Otolaryngology Department at the Harran University Faculty of Medicine between April 2017 and April 2018. Plasmafree amino acid profile levels were studied in serum samples taken from a patient group and a healthy control group. Patients who were diagnosed with bilateral diffuse nasal polyposis and were scheduled for surgical interventions were included in this study. Individuals whose age, gender, and body mass index values were compatible with that of the patient group and who did not have any health problems were included in the control group. All the participants whose levels of plasma-free amino acid were thought to be affected by one or more of the following factors were excluded from the study: smoking and alcohol use, allergic rhinitis presence, the presence of acute or chronic sinusitis, a history of endoscopic sinus surgery, unilateral nasal masses, a history of chronic drug use, systemic or topical steroid use in the last three months for any reason, and liver, kidney, hematological, cardiovascular, metabolic, neurological, or psychiatric disorders or malignancies. Results: In patients with nasal polyposis, 3-methyl histidine (3-MHIS: nasal polyposis group (ng) = 3.22 (1.92 – 6.07); control group (cg) = 1.21 (0.77 – 1.68); p = 0.001); arginine (arg: ng = 98.95 (70.81 – 117.75); cg = 75.10 (54.49 – 79.88); p = 0.005); asparagine (asn: ng = 79.84 (57.50 – 101.44); cg = 60.66 (46.39 – 74.62); p = 0.021); citrulline (cit: ng = 51.83 (43.81 – 59.78); cg = 38.33 (27.81 – 53.73); p = 0.038); cystine (cys: ng = 4.29 (2.43 – 6.66); cg = 2.41 (1.51 – 4.16); p = 0.019); glutamic acid (glu: ng = 234.86 (128.75 – 286.66); cg = 152.37 (122.51 – 188.34); p = 0.045); histidine (his: ng = 94.19 (79.34 – 113.99); cg = 74.80 (62.76 – 98.91); p = 0.018); lysine (lys: ng = 297.22 (206.55 – 371.25); cg = 179.50 (151.58 – 238.02); p = 0.001); ornithine (ng = 160.62 (128.36 – 189.32); cg = 115.91 (97.03 – 159.91); p = 0.019); serine (ser: ng = 195.15 (151.58 – 253.07); cg = 83.07 (67.44 – 92.44); p = 0.001); taurine (tau: ng = 74.69 (47.00 – 112.13); cg = 53.14 (33.57 – 67.31); p = 0.006); tryptophan (trp: ng = 52.31 (33.81 – 80.11); cg = 34.44 (25.94 – 43.07); p = 0.005), homocitrulline (ng = 1.75 (1.27 – 2.59); cg = 0.00 (0.00 – 0.53); p = 0.001); norvaline (ng = 6.90 (5.61 – 9.18); cg = 4.93 (3.74 – 7.13); p = 0.021); argininosuccinic acid (ng = 14.33 (10.06 – 25.65); cg = 12.22 (5.77 – 16.87) p = 0.046); and plasma concentrations were significantly higher than in the healthy control group (p <0.05). However, the gamma-aminobutyric acid (gaba: ng = 0.16 (0.10 – 0.24); cg = 0.21 (0.19 – 0.29); p = 0.010) plasma concentration was significantly lower in the nasal polyposis group than in the healthy control group. Conclusion: In this study, plasma levels of 15 free amino acids were significantly higher in the nasal polyposis group than in the healthy control group. A plasma level of 1 free amino acid was found to be significantly lower in the nasal polyposis group compared to the healthy control group. Therefore, it is important to determine the possibility of using the information obtained to prevent the recurrence of the condition and to develop effective treatment strategies. This study may be a milestone for studies of this subject. However, this study needs to be confirmed by further studies conducted in a larger series.

scholarly journals Putative imbalanced amino acid metabolism in rainbow trout long term fed a plant-based diet as revealed by 1H-NMR metabolomics

2021 ◽  
Vol 10 ◽  
Author(s):  
Catherine Deborde ◽  
Blandine Madji Hounoum ◽  
Annick Moing ◽  
Mickaël Maucourt ◽  
Daniel Jacob ◽  
...  
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Amino Acid Content ◽  
Proton Nuclear Magnetic Resonance ◽  
Free Form ◽  
Metabolomic Analysis ◽  
Nmr Metabolomics ◽  
H Nmr

Abstract The long-term effect of a plant (P)-based diet was assessed by proton nuclear magnetic resonance (1H-NMR) metabolomics in rainbow trout fed a marine fish meal (FM)–fish oil (FO) diet (M), a P-based diet and a control commercial-like diet (C) starting with the first feeding. Growth performances were not heavily altered by long-term feeding on the P-based diet. An 1H-NMR metabolomic analysis of the feed revealed significantly different soluble chemical compound profiles between the diets. A set of soluble chemical compounds was found to be specific either to the P-based diet or to the M diet. Pterin, a biomarker of plant feedstuffs, was identified both in the P-based diet and in the plasma of fish fed the P-based diet. 1H-NMR metabolomic analysis on fish plasma and liver and muscle tissues at 6 and 48 h post feeding revealed significantly different profiles between the P-based diet and the M diet, while the C diet showed intermediate results. A higher amino acid content was found in the plasma of fish fed the P-based diet compared with the M diet after 48 h, suggesting either a delayed delivery of the amino acids or a lower amino acid utilisation in the P-based diet. This was associated with an accumulation of essential amino acids and the depletion of glutamine in the muscle, together with an accumulation of choline in the liver. Combined with an anticipated absorption of methionine and lysine supplemented in free form, the present results suggest an imbalanced essential amino acid supply for protein metabolism in the muscle and for specific functions of the liver.

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