scholarly journals Effect of Calendula officinalis Flower Extract on Acute Phase Proteins, Antioxidant Defense Mechanism and Granuloma Formation During Thermal Burns

2008 ◽  
Vol 43 (2) ◽  
pp. 58-64 ◽  
Author(s):  
Preethi K. Chandran ◽  
Ramadasan Kuttan
Keyword(s):  
Acute Phase ◽  
Antioxidant Defense ◽  
Defense Mechanism ◽  
Acute Phase Proteins ◽  
Granuloma Formation ◽  
Calendula Officinalis ◽  
Thermal Burns ◽  
Flower Extract

Effect of acute phase proteins, especially α2-macroglobulin, on granuloma formation around Schistosoma mansoni eggs in the rat

Parasitology ◽  
1991 ◽  
Vol 102 (1) ◽  
pp. 49-56 ◽  
Author(s):  
J. Van Gool ◽  
D. Van Tiel ◽  
M. J. Doenhoff ◽  
H. Van Vugt
Keyword(s):  
Schistosoma Mansoni ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Granuloma Formation ◽  
Α2 Macroglobulin ◽  
Phase Group ◽  
Mean Size ◽  
The Mean ◽  
Procollagen Iii Peptide ◽  
Liver Collagen

SUMMARYA new model for the study of granuloma formation in the liver is described. Rats received an injection of 20 000 Schistosoma mansoni eggs in the portal vein and granuloma formation was evaluated at 3, 5 and 7 weeks post-injection. Liver collagen was estimated at the same time and serum procollagen III peptide, a marker of collagenesis, weekly. With this model, wherein the number of S. mansoni eggs and the time of injury are standardized, the effect of high levels of acute phase proteins especially α2-macroglobulin on granuloma formation was studied. It appeared that in rats with high levels of α2-macroglobulin the mean size of granulomas was significantly greater at 3 and 5 weeks compared with controls. In both groups an increase in liver collagen was observed during this period, reaching a peak at 5 weeks in the acute phase group. This model facilitates the study of the effects of S. mansoni eggs on granuloma formation.

scholarly journals Acute-phase proteins, oxidative stress, and antioxidant defense in crib-biting horses

2017 ◽  
Vol 20 ◽  
pp. 31-36 ◽  
Author(s):  
Arash Omidi ◽  
Saeede Vakili ◽  
Saeed Nazifi ◽  
Matthew O. Parker
Keyword(s):  
Oxidative Stress ◽  
Acute Phase ◽  
Antioxidant Defense ◽  
Acute Phase Proteins

Keratins 8/18 represent type II acute-phase proteins which are differentially regulated in human liver disease

2013 ◽  
Vol 51 (01) ◽  
Author(s):  
N Güldiken ◽  
V Usachov ◽  
K Levada ◽  
M Ziol ◽  
P Nahon ◽  
...  
Keyword(s):  
Liver Disease ◽  
Acute Phase ◽  
Human Liver ◽  
Acute Phase Proteins ◽  
Type Ii ◽  
Human Liver Disease

scholarly journals Comparison of host endothelial, epithelial and inflammatory response in ICU patients with and without COVID-19: a prospective observational cohort study

Critical Care ◽  
2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Pavan K. Bhatraju ◽  
Eric D. Morrell ◽  
Leila Zelnick ◽  
Neha A. Sathe ◽  
Xin-Ya Chai ◽  
...  
Keyword(s):  
Endothelial Dysfunction ◽  
Epithelial Cell ◽  
Acute Phase ◽  
Cell Injury ◽  
Acute Phase Proteins ◽  
Invasive Mechanical Ventilation ◽  
Icu Patients ◽  
Icu Admission ◽  
Amyloid A ◽  
Epithelial Cell Injury

Abstract Background Analyses of blood biomarkers involved in the host response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral infection can reveal distinct biological pathways and inform development and testing of therapeutics for COVID-19. Our objective was to evaluate host endothelial, epithelial and inflammatory biomarkers in COVID-19. Methods We prospectively enrolled 171 ICU patients, including 78 (46%) patients positive and 93 (54%) negative for SARS-CoV-2 infection from April to September, 2020. We compared 22 plasma biomarkers in blood collected within 24 h and 3 days after ICU admission. Results In critically ill COVID-19 and non-COVID-19 patients, the most common ICU admission diagnoses were respiratory failure or pneumonia, followed by sepsis and other diagnoses. Similar proportions of patients in both groups received invasive mechanical ventilation at the time of study enrollment. COVID-19 and non-COVID-19 patients had similar rates of acute respiratory distress syndrome, severe acute kidney injury, and in-hospital mortality. While concentrations of interleukin 6 and 8 were not different between groups, markers of epithelial cell injury (soluble receptor for advanced glycation end products, sRAGE) and acute phase proteins (serum amyloid A, SAA) were significantly higher in COVID-19 compared to non-COVID-19, adjusting for demographics and APACHE III scores. In contrast, angiopoietin 2:1 (Ang-2:1 ratio) and soluble tumor necrosis factor receptor 1 (sTNFR-1), markers of endothelial dysfunction and inflammation, were significantly lower in COVID-19 (p < 0.002). Ang-2:1 ratio and SAA were associated with mortality only in non-COVID-19 patients. Conclusions These studies demonstrate that, unlike other well-studied causes of critical illness, endothelial dysfunction may not be characteristic of severe COVID-19 early after ICU admission. Pathways resulting in elaboration of acute phase proteins and inducing epithelial cell injury may be promising targets for therapeutics in COVID-19.

scholarly journals Effects of Stocking and Transport Conditions on Physicochemical Properties of Meat and Acute-Phase Proteins in Cattle

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 252
Author(s):  
Ahmed A. Abubakar ◽  
Idrus Zulkifli ◽  
Yong M. Goh ◽  
Ubedullah Kaka ◽  
Azad B. Sabow ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Stocking Density ◽  
Road Transport ◽  
Long Distance ◽  
Amyloid A ◽  
Tropical Conditions ◽  
Meat Samples ◽  
Water Holding

This study’s objective was to evaluate the effects of distance and stocking density on physicochemical properties and oxidative stability of meat and acute-phase proteins in Brahman crossbred cattle transported by road under hot and humid tropical conditions. Sixty Brahman crossbred heifers were subjected to road transport from a cattle feedlot farm located in Universiti Putra Malaysia (UPM), Serdang, to a commercial ruminant abattoir in Shah Alam, Selangor. Animals were assigned to long and short distances and high, medium, and low stocking densities. The results revealed that the intensity of response significantly increased in meat samples from animals subjected to long-distance transportation and higher stocking density. Alpha-1-acid glycoprotein and serum amyloid-A values increased considerably and were different from the baseline values recorded at preload. In conclusion, the current results revealed that the color, pH, shear force values, water holding capacity (WHC), glycogen level, and malondilaldehyde assay (MDA) concentrations in meat and acute-phase proteins (APP) were affected by both distances and stocking densities, as evidenced by the significant changes recorded from the parameters above.

scholarly journals OP0229 GUSELKUMAB INDUCES SUSTAINED REDUCTION IN ACUTE PHASE PROTEINS AND TH17 EFFECTOR CYTOKINES IN ACTIVE PSORIATIC ARTHRITIS IN TWO PHASE-3 CLINICAL TRIALS (DISCOVER-1 AND DISCOVER-2)

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 144-145
Author(s):  
S. Siebert ◽  
I. Mcinnes ◽  
M. J. Loza ◽  
K. MA ◽  
K. Leander ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Geometric Mean ◽  
Tnf Inhibitor ◽  
Healthy Controls ◽  
Phase 3 ◽  
Two Phase ◽  
Protein Levels ◽  
Disease Characteristics ◽  
Effector Cytokines

Background:Guselkumab (GUS), an IL-23 inhibitor monoclonal antibody (Mab) that specifically binds to the IL-23p19 subunit, demonstrated efficacy compared to placebo (PBO) in reducing skin and musculoskeletal signs and symptoms in patients (pts) with active psoriatic arthritis (PsA) in two phase-3 studies, DISCOVER 1 & 2.1,2Previous results from a GUS PsA Phase-2 trial3and Ustekinumab (UST, anti-IL12/23p40 MAb) PsA Phase-3 trials (PSUMMIT 1 & 2)4showed associations of baseline IL-17A, IL-17F, and CRP with baseline disease characteristics, and associations of GUS-induced cytokine reductions with clinical responses.Objectives:To investigate plausible cytokine expression in PsA and alterations after exposure to GUS therapy.Methods:In DISCOVER 1 & 2, pts were treated with GUS 100 mg at Wk 0, 4, then every 8Wks (q8w); GUS 100mg q4w; or matching PBO. 21 serum biomarkers were measured in a random subset of 300 PsA pts from the DISCOVER program at Weeks (Wks) 0, 4, & 24 and in 34 healthy controls matched for age, sex, and ethnicity. Serum proteins measured were acute phase reactants CRP & SAA (Meso Scale Discovery (MSD) Platform) and inflammatory cytokines/chemokines: Th17 effector cytokines IL-17A, IL-17F, & IL-22 (Single Molecule Counting Erenna® Immunoassay Platform) and soluble ICAM-1, soluble VCAM-1, IL-6, CXCL-8, IL-10, IL-13, IL-12p70, CCL22, IFN-γ, CCL2, CCL4, TNFα, IL-1β, IL-2, IL-4 (MSD), & YKL-40 (Quantikine Immunoassay). Serum IL-17A, IL-17B, & CRP measured in the Phase-3 PSUMMIT trials of UST for PsA4were included for comparison with GUS.Results:At baseline, serum levels of acute phase proteins CRP, SAA, & IL-6, and Th17-effector cytokines IL-17A & IL-17F were elevated in pts with PsA compared with healthy controls (p<0.05, geometric mean ≥ 40% higher, FIG 1). There was no significant dysregulation in the other cytokines measured in PsA pts compared to healthy controls. Baseline IL-17A, IL-17F, IL-22, & CCL22 were significantly associated with baseline psoriasis disease activity (Body Surface Area & Psoriatic Area and Severity Index, Spearman Signed Rank p<0.05, r>0.25). Baseline CRP, SAA, IL-6, & YKL40 were significantly associated with baseline joint disease (Disease Activity Score 28-CRP, Spearman p<0.05, r>0.25). Baseline SAA, IL-6, IL-17A, & IL-17F were higher in pts with prior TNF inhibitor exposure than without (p<0.05, geometric mean ≥ 40% higher), although pts with PsA both with and without prior TNF inhibitor had higher levels than the healthy control set.GUS treatment resulted in decreases in serum CRP, SAA, IL-6, IL-17A, IL-17F, & IL-22 that were significantly greater than PBO as early as Week 4 (FIG 1). These protein levels continued to decrease through Wk 24 in GUS-treated pts with both dosing regimens (p<0.05, geometric mean decrease from baseline ≥ 33%). Further, Wk 24 IL-17A & IL-17F levels for pts treated with either dose of GUS were not significantly different from healthy controls, suggesting a normalization of peripheral effector cytokines associated with the IL-23/Th17 axis following treatment with GUS. Effects on IL-17A/IL-17F were greater in GUS treated pts than UST treated pts, while CRP levels were similar in both programs (FIG 2).Conclusion:Comprising a strong pharmacodynamic effect, GUS treatment reduced serum protein levels of acute phase and Th17-effector cytokines (whose elevations at baseline were associated with PsA disease characteristics) and achieved comparable levels to those in healthy controls. In pts with PsA, reductions of IL-17A and IL-17F by GUS were of greater magnitude than those by UST.References:[1]Deodhar et al. ACR 2019, abs #807. Arth Rheumatol. 2019;71 S10: 1386[2]Mease et al. ACR 2019, abs #L13. Arth Rheumatol. 2019;71 S10:5247[3]Siebert et al. EULAR 2019, abs #479. Ann Rheum Dis. 2019;78 S2:293[4]Siebert et al. Arth Rheumatol. 2019;71:1660Acknowledgments:NoneDisclosure of Interests:Stefan Siebert Grant/research support from: BMS, Boehringer Ingelheim, Celgene, GlaxoSmithKline, Janssen, Novartis, Pfizer, UCB, Consultant of: AbbVie, Boehringer Ingelheim, Janssen, Novartis, Pfizer, UCB, Speakers bureau: AbbVie, Celgene, Janssen, Novartis, Iain McInnes Grant/research support from: Bristol-Myers Squibb, Celgene, Eli Lilly and Company, Janssen, and UCB, Consultant of: AbbVie, Bristol-Myers Squibb, Celgene, Eli Lilly and Company, Gilead, Janssen, Novartis, Pfizer, and UCB, Matthew J Loza Employee of: Janssen Research & Development, LLC, Keying Ma Employee of: Janssen Research & Development, LLC, Karen Leander, Employee of: Janssen Research & Development, LLC, Vani Lakshminarayanan Employee of: Janssen Research & Development, LLC, Carol Franks Employee of: Janssen Research & Development, LLC, Philip Cooper Employee of: Janssen Research & Development, LLC, Kristen Sweet Employee of: Janssen Research & Development, LLC

scholarly journals Design of Sodium Alginate/Gelatin-Based Emulsion Film Fused with Polylactide Microparticles Charged with Plant Extract

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 745 ◽  
Author(s):  
Weronika Prus-Walendziak ◽  
Justyna Kozlowska
Keyword(s):  
Sodium Alginate ◽  
Plant Extract ◽  
Contact Angles ◽  
Polymeric Films ◽  
Solvent Evaporation Method ◽  
Calendula Officinalis ◽  
Flower Extract ◽  
Polymeric Microparticles ◽  
Emulsion Film ◽  
Emulsion Films

This study aimed at designing emulsion films based on sodium alginate, gelatin, and glycerol, and their modification by the addition of lipids (cottonseed oil and beeswax). Film composition with the most promising properties was further modified by the incorporation of polylactide (PLA) microparticles with Calendula officinalis flower extract. PLA microspheres were obtained by the emulsion/solvent evaporation method. The size distribution of oily particles in emulsions was investigated. Mechanical properties, moisture content, UV-Vis spectra, and the color of films were analyzed, while biophysical skin parameters were assessed after their application to the skin. Moreover, the contact angles were measured, and the surface free energy of polymeric films was determined. An investigation of the amount of Calendula officinalis flower extract which can be incorporated into PLA microparticles was performed. The modification of the composition of films significantly influenced their physicochemical properties. The selected active ingredient in the form of plant extract was successfully incorporated into polymeric microparticles that were further added into the developed emulsion film. The condition of the skin after the application of obtained emulsion films improved. The prepared materials, especially containing microparticles with plant extract, can be considered for designing new cosmetic forms, such as cosmetic masks, as well as new topical formulations for pharmaceutical delivery.

scholarly journals Responses of Peppermint and Spearmint Crops to Excessive Biostimulant Application and Increased Salinity in a Closed Soilless Production System

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1168
Author(s):  
Dimitrios S. Kasampalis ◽  
Danai-Christina Aktsoglou ◽  
Pavlos Tsouvaltzis ◽  
Anastasios S. Siomos
Keyword(s):  
Amino Acid ◽  
Osmotic Stress ◽  
Antioxidant Defense ◽  
Defense Mechanism ◽  
Substantial Improvement ◽  
Oxygen Species ◽  
Floating System ◽  
High Salt Tolerance ◽  
Heated Glass ◽  
Nutrient Solutions

A floating system was established in a heated glass greenhouse in order to investigate whether the effect of amino acids (0.25 or 0.50% of a commercial amino acid (AA) solution Amino16®) during peppermint and spearmint production on plant developmental and nutritional status may be in part attributed to salinity induced osmotic stress. For this reason, in some nutrient solutions, three levels of salinity were induced by adding 0, 10, or 20 mM NaCl. According to the results, it can be concluded that spearmint is mostly favored by the highest amino acid supplement of the nutrient solution (0.50%) in terms of a substantial improvement of the antioxidant nutritional quality (by up to 130%) at the expense of a reduced biomass production (by <30%). Enzymic antioxidant defense mechanism (APX and POD) was efficiently activated, preventing severe lipid peroxidation and the accumulation of reactive oxygen species such as H2O2 and maintaining the proline content at the normal levels. The osmotic stress that was induced by the excessive AA concentration and confirmed by the chlorophyl fluorescence variations was probably related to NH4+ excess supply in the growing media and was not associated with the elevated electrical conductivity in the solution. The absence of any adverse stressful consequences upon the addition of 20 mM NaCl may be attributed to the high salt tolerance of peppermint and spearmint species.

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