Effect of Pomegranate Peel Extract onCandida albicansGrowth and Biofilm Formation

2018 ◽  
Vol 17 (3) ◽  
pp. 120-128
Author(s):  
H. Merzouk ◽  
B. Bedja ◽  
B. Benmeziane ◽  
N. Touati ◽  
M. Chibane
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Qualitative Assessment ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Fruit Peel ◽  
Pomegranate Peel ◽  
Total Polyphenols ◽  
Pomegranate Peel Extract ◽  
Peel Extract

In the present work, we studied the potential antioxidant capacity and antibiofilm of pomegranate peel extract of two cultivars againstCandida albicansstrains. First, both pomegranate peel extracts were evaluated to determine their total phenolic and tannin contents. Their antioxidant activity was investigated using hydrogen peroxide scavenging assay. Acid fruit peel extract showed the most significant levels of total polyphenols (513.78 ± 1.552 mg GAE/g DE) and possessed the strongest antioxidant ability (96.32%). Next, the possible effect of the extracts onCandida albicansstrains was studied using agar diffusion method and subjected to various extracts concentrations. The zone sizes of growth inhibition showed clearly that these extracts had a good antifungal activity againstCandida albicansspecies in the following order: CA1 > CA3 > CA2 with minimum inhibitory concentrations (MICs) in the range 0.97–1.94 mg/ml. Images obtained by scanning electron microscopy allowed for a qualitative assessment of the biofilm reduction.

Development and Evaluation of a Novel Oral Mucoadhesive Ointment Containing Pomegranate Peel Extract as an Adjuvant for Oral Hygiene of Dogs

2020 ◽  
Vol 37 (3) ◽  
pp. 133-140
Author(s):  
Thais M. Silva ◽  
Thays C. A. Bolzan ◽  
Marcos S. Zanini ◽  
Taiana Alencar ◽  
Winner D. Rodrigues ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Oral Hygiene ◽  
Diffusion Method ◽  
Pomegranate Peel ◽  
Ointment Formulation ◽  
Microbiological Testing ◽  
Herbal Therapies ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Herbal therapies are used worldwide to treat a variety of health conditions, including dental conditions in veterinary medicine. In this context, the use of medicinal plant-based formulations as potential therapeutics and preventatives in veterinary dentistry is worth highlighting. The objective of the present study was to develop a mucoadhesive ointment formulation, named orabase, that contained pomegranate extract for use in the oral cavity of dogs, with the aim of improving their oral hygiene. The hydroalcoholic extracts of pomegranate peels was incorporated into the orabase in 3 different concentrations. The formulations were subjected to in vitro microbiological testing by a modified disc-diffusion method to study the susceptibility of microorganisms collected from the oral cavities of the dogs. The samples were taken from the buccal mucosa of dogs having the same management and diet. The most effective formulation was submitted to physicochemical tests to evaluate the functionality of the product, namely pH, swelling index, spreadability, and mechanical properties (hardness, cohesiveness, and adhesiveness). The formulation containing 25.0% w/w of the extract was considered most suitable for the intended use as it showed antiseptic activity and demonstrated a swelling index of approximately 35% in the first 20 minutes of the test, high spreadability, and suitable mechanical properties. The results suggest that the product obtained from pomegranate peel extract is a viable option for use to improve oral hygiene, helping to reduce the bacterial component of dental plaque in dogs.

scholarly journals Activity of Spray-dried Microparticles Containing Pomegranate Peel Extract against Candida albicans

Molecules ◽  
2012 ◽  
Vol 17 (9) ◽  
pp. 10094-10107 ◽  
Author(s):  
Eliana Harue Endo ◽  
Tânia Ueda-Nakamura ◽  
Celso Vataru Nakamura ◽  
Benedito Prado Dias Filho
Keyword(s):  
Candida Albicans ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Peel Extract ◽  
Spray Dried

scholarly journals Aktivitas Antimikroba Ekstrak Kulit Buah Kelengkeng (Euphoria longan Stend) Terhadap Pertumbuhan Candida albicans Dan Propionibacterium acne

Media Farmasi ◽  
2020 ◽  
Vol 16 (2) ◽  
pp. 155
Author(s):  
Alfrida Monica Salasa ◽  
St Ratnah
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Compounds ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Fruit Peel ◽  
Propionibacterium Acne ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Peel Extract

Longan Fruit (Euphoria longan Stend) Peel Waste contains chemical compounds with antimicrobial activity, which are not fully utilized. The study aims to determine the inhibition of Longan Fruit Peel extract (Euphoria longan Stend) against Candida albicans and Propionibacterium acne. Furthermore, Longan fruit (Euphoria longan Stend) was dried and extracted by maceration method using 96% ethanol, then tested for its antimicrobial activity by agar diffusion method at a concentration of 5% 7.5% and 10% w/v. The results showed that the average inhibition zone diameter for Candida albicans at 5% concentration was 14.33 mm, 7.5% concentration was 15.33 mm, and 10% concentration was 16.66 mm. Meanwhile, the inhibition zone diameter for Propionibacterium acne at a concentration of 5% w/v was 14.67 mm, 7.5% w/v was 16.67 mm, and 10% w/v was 18.33 mm. The Longan Fruit Peel Extract (Euphoria longan Stend) has antimicrobial activity tested against Candida albicans at a concentration of 7.5% w/v and 10% w/v for Propionibacterium acne. Keywords: Longan Fruit Peel Extract, Antimicrobial activity, Candida albicans, Propionibacterium acneLimbah Kulit Buah Kelengkeng (Euphoria longan Stend) mengandung senyawa kimia yang memiliki aktivitas sebagai antimikroba, namun sampai saat ini belum dimanfaatkan secara maksimal. Tujuan penelitian ini untuk menentukan daya hambat ekstrak Kulit Buah kelengkeng (Euphoria longan Stend) terhadap Candida albicans dan Propionibacterium acne. Kulit buah kelengkeng (Euphoria longan Stend) dikeringkan kemudian diekstraksi dengan metode maserasi dengan menggunakan Etanol 96% lalu diuji aktivitas antimikrobanya dengan metode difusi agar pada konsentrasi 5%; 7,5% dan 10%b/v. Hasil pengujian menunjukkan  rata-rata diameter zona hambat untuk Candida albicans pada konsnetrasi 5% sebesar 14,33 mm, konsentrasi 7,5% sebesar 15,33 mm, konsentrasi 10% sebesar 16,66 mm. Sedangkan diameter zona hambat untuk Propionibacterium acne pada konsentrasi 5% b/v sebesar 14,67 mm, 7,5% b/v sebesar 16,67 mm, dan  konsetrasi 10% b/v sebesar 18,33 mm  Hasil uji statistik menunjukkan Ekstrak Kulit Buah kelengkeng (Euphoria longan Stend) memiliki aktivitas antimikroba terhadap Candida albicans pada konsentrasi 7,5% b/v dan konsentrasi 10%b/v untuk Propionibacterium acne.Kata Kunci : Ekstrak Kulit Buah Kelengkeng, Aktivitas antimikroba, Candida albicans, Propionibacterium acne

Physicochemical Properties and Antimicrobial Potential of Green Synthesized Cerium Oxide (CeO2) Nanoparticles from Pomegranate Peel Extract

2021 ◽  
Vol 14 (1) ◽  
pp. 71-78
Keyword(s):  
Antimicrobial Activity ◽  
Biomedical Applications ◽  
Punica Granatum ◽  
Ceo2 Nanoparticles ◽  
X Ray Diffraction ◽  
Fruit Peel ◽  
Pomegranate Peel ◽  
X Ray ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Abstract: Green synthesis of CeO2 Nanoparticles (NPs) with small size and high stability paved the approach to recover and protect the environment by decreasing the use of toxic chemicals and eliminating biological risks in biomedical applications. Peel-mediated synthesis of CeO2 NPs is gaining more importance owing to its easiness and eco-friendliness. In this study, biosynthesis of CeO2 NPs using the fruit peel extract of punica granatum is reported. The synthesized CeO2 NPs are characterized by Powder X-ray Diffraction (PXRD), UV-Diffused Reflection Spectroscopy (UV-DRS), Field Emission Scanning Electron Microscopy (FESEM), Energy Dispersive X-Ray Analysis (EDAX) and antimicrobial activity. The CeO2 NPs show more lethal activity towards gram +ve bacteria than towards gram –ve bacteria. Keywords: Biosynthesis, Optical properties, Antimicrobial activity.

Phenolic and Flavonoids Analysis of Pomegranate Peel Extracts and their Antiniflammatory and Antioxidant Activities

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Qabaha K. ◽  
Al-Rimawi F ◽  
Nusseibeh S. ◽  
Abbadi J J. ◽  
Abu-Lafi S.
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Polymorphonuclear Cells ◽  
Pomegranate Peel ◽  
Radical Scavenging Ability ◽  
Pomegranate Peel Extract ◽  
Peel Extract

An in-vitro evaluation of the anti-inflammatory and antioxidant activities of pomegranate peel extract from Palestine were investigated. In parallel, the total phenolic content (TPC) and the total flavonoids content (TFC) were measured. The antioxidant activities were determined spectrophotometrically by DPPH, FRAP, CUPRAC and the ABTS methods. The phenolic and flavonoid contents were separated and partially identified using HPLC and LC-MS. In-vitro inhibitory effect of the extract on production of Interlukin-6 (Il-6) and Tumor Necrosis Factor-α (TNF-α) by Lipopolysacaride (LPS)-induced polymorphonuclear Cells (PMNCs) was evaluated. Pomegranate peel extract was found to have strong antiinflamatory activity as revealed by the reduction in the levels of IL-6 and TNF-alfa. It was also found that it is rich in phenoloic and flavonoids that enhanced its reducing activity and free radical scavenging ability.

scholarly journals Stabilization of rose hip oil by pomegranate peel extract during accelerated storage

2020 ◽  
pp. 64-64
Author(s):  
Jelena Mudric ◽  
Zorica Drinic ◽  
Gordana Zdunic ◽  
Marina Todosijevic ◽  
Dubravka Bigovic ◽  
...  
Keyword(s):  
Antiradical Activity ◽  
Phenolic Content ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Synthetic Antioxidant ◽  
Pomegranate Peel ◽  
Rose Hip ◽  
The Stability ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Rose hip seed oil is a rich source of polyunsaturated fatty acids, as well as tocopherols, carotenoids, sterols, phospholipids, and phenolic compounds. On the other hand, due to the high content of polyunsaturated fatty acids this oil is prone to oxidation. The aim of this study was to investigate the influence of natural antioxidant such as pomegranate peel extract and its combination with butylated hydroxytoluene as commonly used synthetic antioxidant, on the stability of rose hip oil. The stability of samples without and with different antioxidants was monitored through fatty acid composition analysis and measuring the quality and stability parameters of the oil (peroxide value, p-anisidine value, thiobarbituric acid reactive substances inhibition, total phenolic content and antiradical activity) during the storage period of 12 days at 65?C. Pomegranate peel extract (0.1 %) inhibited more effectively the second stage of oxidation than butylated hydroxytoluene (0.02 %), while the first stage of oxidation was prevented better by synthetic antioxidant. Further-more, the addition of pomegranate peel extract increased the total phenolic content of the rose hip oil as well as its antiradical activity. Thus, pomegranate peel extract can be used as a potent natural antioxidant for stabilization of beneficial but unstable rose hip oil.

Characterization of Antioxidant Chitosan Film Incorporated with Pomegranate Peel Extract

2013 ◽  
Vol 706-708 ◽  
pp. 24-27 ◽  
Author(s):  
Jian Fan ◽  
Zhi Hong Zhang ◽  
Yu Yue Qin ◽  
Tian Rui Zhao ◽  
Chun Sheng Cheng
Keyword(s):  
Antioxidant Activity ◽  
Water Vapor ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Chitosan Film ◽  
Total Phenolic ◽  
Vapor Permeability ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Peel Extract

A novel antioxidant chitosan film incorporated with pomegranate peel extract (PPE) (0, 1%, 2%, and 3% (w/v)) was developed. The mechanical property, water vapor permeability, total phenolic content and antioxidant activity of the films were investigated. Fourier transform infrared (FTIR) spectra revealed that good interactions occurred between functional groups of chitosan with PPE. The flexibility and water vapor barrier property were improved by incorporation with PPE. Films containing PPE had higher total phenolic content and showed better antioxidant activity. These results suggest that chitosan films incorporated with PPE can be useful for meat preservation.

scholarly journals Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction

2021 ◽  
Vol 11 (3) ◽  
pp. 375-382
Author(s):  
Laela Wulansar ◽  
◽  
Dewi Septaningsih ◽  
Tsania Purnomo ◽  
Reza Auliatifani ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Ftir Spectrum ◽  
Total Phenolic ◽  
Fingerprint Analysis ◽  
Pomegranate Peel ◽  
Phytochemical Profile ◽  
Pomegranate Peel Extract ◽  
Phenolic And Flavonoid ◽  
Peel Extract

Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 µmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26–1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.

scholarly journals In vitro activity of pomegranate peel extract alone and in electrospun poly(vinyl alcohol)/sodium alginate matrix

2020 ◽  
Vol 17 (2) ◽  
pp. 1-19
Author(s):  
Sanderson Vieira Batista ◽  
Thais Martins da Silva ◽  
Sthefany Brito Salomão ◽  
Kamila Arêas Bastos ◽  
Sarah Oliveira Lamas de Souza ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Wound Dressings ◽  
Diffusion Method ◽  
Poly Vinyl Alcohol ◽  
Bacterial Strains ◽  
Pomegranate Peel ◽  
Electrospinning Technique ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Wound dressings can support healing by acting as physical barriers and drug delivery systems. The present study aimed to evaluate the biological activity of films containing pomegranate peel extract at 1.25% w/w prepared by the electrospinning technique for use as wound dressing. The extract was subjected to an in vitro cytotoxicity assay using fibroblasts. The formation of fibers was confirmed using scanning electron microscopy. Biocompatibility was analyzed by determining the hemolysis index, while the susceptibility of bacterial strains and a fungus was investigated using the disc diffusion method. The extract proved to be nontoxic with cell proliferation occurring at a concentration > 30 µg·mL-1. Photomicrograph images confirmed the formation of nanofibers. Films without or with the extract showed good hemocompatibility. The antimicrobial activity of films loaded with extract was demonstrated by the formation of zones of inhibition of Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans, suggesting that the release of the extract in the medium occurred at an adequate concentration to inhibit the proliferation of microorganisms. No zone of inhibition was observed for Escherichia coli. These results suggest that the film produced by electrospinning containing 1.25% w/w of the extract might serve as a bioactive wound dressing.

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