scholarly journals THE DEVELOPMENT OF MEMBRANOUS PATHOLOGY OF HEPATOCYTES THE INFLUENCE OF INTOXICATION

2018 ◽  
Vol 22 (3-4) ◽  
pp. 22-24
Author(s):  
I.Yu. Bagmut ◽  
I.L. Kolisnyk ◽  
A.V. Titkova ◽  
Yu.K. Rezunenko ◽  
O.D. Boiagina
Keyword(s):  
Lipid Peroxidation ◽  
Sodium Fluoride ◽  
Postgraduate Education ◽  
Mitochondrial Respiratory Chain ◽  
Control Group ◽  
Monooxygenase System ◽  
Diene Conjugates ◽  
Operative Surgery ◽  
Long Time ◽  
State Registration

The study was performed at the Department of Clinical Pathophysiology, Topographical Anatomy and Operative Surgery at Kharkiv Medical Academy of Postgraduate Education as a part of research project "Radiotoxins' pathochemical mechanisms and methods of early diagnostics and correction", state registration No. 0117U000589. We studied the subtoxic effect of small doses of sodium fluoride on the activity of microsomal hepatocytes on 30 Wistar rats' populations in subacute experiment. The intensity of lipid peroxidation (LPO) in liver of rats which were administered sodium fluoride orally for a long time at doses of 1/10 and 1/100 LD50, was evaluated by the content of its molecular products - diene conjugates (DC), and MDA-reagents Schiff bases. We found that oral administration of sodium fluoride to rats at doses of 1/10 and 1/100 LD50 promotes a statistically significant increase (r≤0,002) relative to the control group of animals in DC content during the entire period of observation. At a dose 1/10 LD50 we observed the most significant increase in this indicator on the 10th day of the experiment - at 265%, and at a dose 1/100 LD50 – on the 20th day an average of 234%. In rats’ liver by the action of subtoxical dose of sodium fluoride at a dose of 1/10 LD50, starting from the 20th day, we detected a gradual increase (r≤0.001) of TBA-reagents relative to control - for 27, 41, 78, 133%. Secondary end products and lipid peroxidation, which are defined under the long-term of sodium fluoride, somehow contribute to the disruption of the microstructure of hepatocytes membranes, their permeability, reduce their division, regeneration and inhibition of mitochondrial respiratory chain enzymes and microsomal monooxygenase system.

scholarly journals In Vivo Protective Effects of Gallic Acid Isolated from Peltiphyllum Peltatum Against Sodium Fluoride-Induced Oxidative Stress in Rat Erythrocytes

2013 ◽  
Vol 64 (4) ◽  
pp. 553-559 ◽  
Author(s):  
Seyed Fazel Nabavi ◽  
Solomon Habtemariam ◽  
Antoni Sureda ◽  
Akbar Hajizadeh Moghaddam ◽  
Maria Daglia ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Vitamin C ◽  
Gallic Acid ◽  
Sodium Fluoride ◽  
Enzyme Activities ◽  
Control Group ◽  
Rat Erythrocytes ◽  
Pre Treatment

Abstract Gallic acid has been identified as an antioxidant component of the edible and medicinal plant Peltiphyllum peltatum. The present study examined its potential protective role against sodium fluoride (NaF)-induced oxidative stress in rat erythrocytes. Oxidative stress was induced by NaF administration through drinking water (1030.675 mg m-3 for one week). Gallic acid at 10 mg kg-1 and 20 mg kg-1 and vitamin C for positive controls (10 mg kg-1) were administered daily intraperitoneally for one week prior to NaF administration. Thiobarbituric acid reactive substances, antioxidant enzyme activities (superoxide dismutase and catalase), and the level of reduced glutathione were evaluated in rat erythrocytes. Lipid peroxidation in NaF-exposed rats significantly increased (by 88.8 %) when compared to the control group (p<0.05). Pre-treatment with gallic acid suppressed lipid peroxidation in erythrocytes in a dose-dependent manner. Catalase and superoxide dismutase enzyme activities and glutathione levels were reduced by NaF intoxication by 54.4 %, 63.69 %, and 42 % (p<0.001; vs. untreated control group), respectively. Pre-treatment with gallic acid or vitamin C significantly attenuated the deleterious effects. Gallic acid isolated from Peltiphyllum peltatum and vitamin C mitigated the NaF-induced oxidative stress in rat erythrocytes.

scholarly journals Impact of lead acetate and sodium and potassium stearates on lipid peroxidation processes in the body of experimental animals

2021 ◽  
Vol 100 (4) ◽  
pp. 406-410
Author(s):  
Olha Ye. Fedoriv ◽  
Alexandra Ye. Kopach ◽  
Nataliia A. Melnyk
Keyword(s):  
Lipid Peroxidation ◽  
Drinking Water ◽  
Lead Acetate ◽  
Sodium Stearate ◽  
The Body ◽  
Female Rats ◽  
White Female ◽  
Control Group ◽  
Diene Conjugates ◽  
Group 2

Introduction. Given the significant prevalence of lead in the environment, research in this area has significant social and economic importance. Lead compounds are characterized by high toxicity and increased ability to cumulate in ecosystems, humans, and animals. Lead enters the human body with food, drinking water, atmospheric air, and smoking. Lead causes pathological changes in the nervous system, blood-forming organs, kidneys, etc. Materials and methods. The experiments were carried out on four groups of white female rats, each included seven animals, weighing 150-200 g. The first group of animals was a control. The second group consumed dechlorinated water from the city water supply, followed by lead acetate. The animals from the third and fourth groups drank the same water with sodium stearate and potassium stearate content in a dose of 1/250 LD50. After the 40th-day of the use of these waters, the animals were orally administered lead acetate at a dose of 7 mg/kg. The levels of lipid peroxidation biomarkers were studied by studying the content of diene conjugates (DC) and malondialdehyde (MDA) in blood serum, liver, and kidney homogenates. Results. The administration of 1/2 acetate LD50 to lead in experimental rats drinking water with stearates was accompanied by a significant increase in the DCs concentration and (MDA) in animals. Higher concentrations of LPO products were observed in the group of animals that consumed water from potassium stearate. Conclusions. 1. With the oral administration of lead acetate against the background of drinking water containing stearates at a dose of 1/250 LD50, an increase in lipid peroxidation indices was noted compared with the control group. 2. Higher concentrations of LPO products were observed in the group of animals consuming water from potassium stearate.

scholarly journals CONTENT OF PRIMARY AND SECONDARY LIPID PEROXIDATION PRODUCTS IN SUBCELLULAR FRACTIONS OF CARDIOMYOCYTES DURING MYOCARDIAL INFARCTION IN RATS IN AN EXPERIMENT AND THEIR CORRECTION BY TRANSPLANTATION OF MESENCHYMAL STEM CELLS

2021 ◽  
Vol 11 (4) ◽  
pp. 89-92
Author(s):  
Vyacheslav Mykhaylichenko ◽  
Andrey Pilipchuk ◽  
Dmitry Parshin ◽  
Yuri Kostyamin
Keyword(s):  
Myocardial Infarction ◽  
Lipid Peroxidation ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Subcellular Fractions ◽  
Control Group ◽  
Diene Conjugates ◽  
Mesenchymal Stem Cell Transplantation ◽  
Lipid Peroxidation Products ◽  
Group I

Experimental modeling of myocardial infarction in rats was carried out by ligation of the anterior intergastric artery after the first division. There were 3 groups of 20 animals each: control group I — to verify normal parameters, group II — a model of myocardial infarction, and group III — animals which, after modeling myocardial infarction, underwent transplantation of mesenchymal stem cells. The level of lipid peroxidation products — diene conjugates and malondialdehyde — was studied by spectrophotometry. Comparison of the content and their ratio in the cytoplasm and mitochondria of myocardiocytes was carried out. It turned out that transplantation of mesenchymal stem cells significantly levels the activation of lipid peroxidation processes in subcellular fractions of cardiomyocytes, which is accompanied by a decrease in the primary and secondary products of oxidative stress. The ratio of malondialdehyde to diene conjugates both in the cytoplasm and in the mitochondria of cardiomyocytes after transplantation returned to control values. This indicates the normalization of physiological processes with underlying ischemic heart damage. The results indicate the cytoprotective effect of mesenchymal stem cell transplantation and the preservation of a larger number of cell pools, compared with the control group of animals that did not receive any treatment.

Immunomodulatory and Antioxidant Action of Selective Inhibitor of Arginase IInor-NOHA and Antihypoxants in Some Extreme Conditions

2015 ◽  
Vol 22 (3) ◽  
pp. 161-164
Author(s):  
Лосенок ◽  
P. Losenok ◽  
Наседкин ◽  
D. Nasedkin ◽  
Квачахия ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Selective Inhibitor ◽  
Control Group ◽  
Extreme Conditions ◽  
Secondary Products ◽  
Diene Conjugates ◽  
Pharmacological Agents ◽  
Combined Application ◽  
Arginase Ii

The study of the effects of a selective inhibitor of arginase II nor-NOHA and its combinations with Hy-poxenum, Kudesan, Cytoflavin on the functional activity of neutrophils, lipid peroxidation and content of stable metabolites of nitrogen in the blood serum of rats under the influence of some extreme conditions was carried out. It was found that under conditions of hypoxia that occurs in some extreme situations, there is a reduction of spontaneous and induced phagocytic and metabolic activity of neutrophils. Combined application of nor-NOHA, hypoxen and cytoflavin increases spontaneous PMA of neutrophils to values of the control group, but does not influence the values of NST Ind. The authors found that at hypoxia, there is an increase in the content of primary and secondary products of lipid peroxidation. Administration nor-NOHA and antihypoxants reduces the concentration of malondialdehyde at all combinations of drugs, and reduction of diene conjugates only in animals treated with nor-NOHA, hypoxen and cytoflavin. Total antioxidant activity increased as well, for all combinations of pharmacological agents, and catalase activity was increased combinations nor-NOHA + cytoflavin and nor-NOHA + hypoxen + cytoflavin. At hypoxia, there is a significant increase of stable NO metabolites in blood serum. Introduction nor-NOHA further increases their concentration, and at different combinations antigipoksantov administered in combination with an inhibitor of arginase II nor-NOHA marked reduction of NO metabolite to an animal in a state of hypoxia.

scholarly journals THE INTENSITY OF LIPID PEROXIDATION PROCESSES IN WOMEN WITH PRIMARY VARICOSE VEINS OF THE PELVIC DEPENDING ON THE STAGE OF THE DISEASE

2018 ◽  
Vol 73 (4) ◽  
pp. 229-235 ◽  
Author(s):  
Lyubov’ I. Kolesnikova, ◽  
Andrey A. Semendyaev ◽  
Dmitriy A. Stupin ◽  
Marina A. Darenskaya ◽  
[Lyudmila A. Grebenkina ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Defense ◽  
Varicose Veins ◽  
Reproductive Age ◽  
Background Information ◽  
Control Group ◽  
Maximum Increase ◽  
Diene Conjugates ◽  
Primary Varicose Veins ◽  
Peroxidation Processes

Background: Information about involvement of general nonspecific reactions, in particular lipid peroxidation processes, in the progression of varicose veins of the pelvic (VVP) in women is still too scarce. Aims: To study the intensity of processes of lipid peroxidation and the activity of components of the antioxidant system in women with primary varicose veins of the pelvic at different stages of the disease. Materials and methods: 167 women of reproductive age were examined ― 137 with VVP and 30 made up a control group. All patients with VVP were divided into 3 groups depending on stages of the disease. Spectrophotometric and fluorometric methods of investigation were used. The study was conducted during 2012−2017. Results: It was revealed that the level of primary products of lipid peroxidation, diene conjugates, increased statistically significantly according to the stage of the disease by 1.25 times (in the 1st stage), 1.51 times (in the II stage) and 1.59 times (in the III stage) values. Changes in the content of final TBA-active products showed similar changes-an increase in the mean values for all stages of the disease relative to control (in 1.24, 1.17, and 1.77 times, respectively). Activity of glutathione peroxidase increased in stage 2 of VVP (1.19 times), with the maximum increase in stage III (1.42 times); activity of glutathione-S-transferase increased 1.18 times in the II stage of the disease. The concentration of GSH in the clinical groups was characterized by lower values with respect to the control (by 1.22 times in the 1st stage, in 1.64 times in the II stage), with the maximum decrease of this parameter in the III stage of VVP (3.67 times). The level of catalase activity increased in the I stage of VVP ― by 1.18 times and decreased in the III stage ― by 1.14 times with respect to the control. The activity of SOD showed similar changes with catalase ― in the form of increased activity at the 1st stage (1.35 times higher) and decreased values for II (1.35 times lower) and III (1.65 times lower) for the stages of VVP to the values of control. Conclusions: At progression of primary VVP in women (from the initial stage to the 3rd stage of the disease), there is an increase in imbalance in the lipid peroxidation − antioxidant defense system. Moreover, if the compensatory increase in activity of antioxidant enzymes is registered at stage 1 of the disease, then the most of the antioxidant defense factors decreases as relative to control values, and the initial stages of the disease.

scholarly journals The intensity of the processes of lipid peroxidation in the blood of cows in experimental fasciolosis

2020 ◽  
Vol 22 (100) ◽  
pp. 151-155
Author(s):  
D. V. Freiuk ◽  
V. V. Stybel
Keyword(s):  
Lipid Peroxidation ◽  
Economic Damage ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Diene Conjugates ◽  
Metabolic Products ◽  
End Products ◽  
Experimental Infestation ◽  
Peroxidation Processes ◽  
Experimental Group

Among the most common pathologies of parasitic etiology in ruminants, trematode diseases, namely fasciolosis, occupy a prominent place in terms of the degree of damage and economic damage caused. Activation of lipid peroxidation processes plays a significant role in the mechanisms of the development of fasciolosis in animals. The research aimed to investigate the level of lipid peroxidation products in cows' blood in experimental fasciolosis. For the test, 12 cows of 4–5 years of age, black-spotted breed, were selected, from which 2 groups were formed, six animals in each. Animals in the control group were clinically healthy. Animals of the experimental group were experimentally infected with adolescents. During the research, the rules of compulsory experiments were followed – selecting and keeping analog animals in groups. The cows' diet was balanced in terms of nutrients and minerals. Based on the studies, it was found that after the experimental infestation of cows of the experimental group with the causative agent of fasciolosis, an increase in the level of diene conjugates of their blood on the 15th day of the experiment by 30.3 %, and on the 25th day – by 50.4 % relative to the control group. There was also an increased level of lipid hydroperoxides in their blood, where, respectively, on the 10th and 15th day of the experiment, this figure increased by 18.3 and 32.9 % compared with the control group. On the 25th day of the experiment, the level of lipid hydroperoxides in the blood of cows of the experimental group was the highest. Compared with the control group, it increased by 46.6%, respectively. In the study of the final products of lipid peroxidation, it was found that on the 10th day of the test, the level of TBA-active products in infected cows of the experimental group increased by 9.5 %. On the 15th and 20th days of the experiment, the level of end products of LPO in the experimental group's blood of animals was higher by 20.3 and 26.0 % relative to the control group. The increase in TBA-active products' level reflects the activation of peroxide processes in animals with experimental fasciolosis. Therefore, in the clinical manifestation of fasciolosis, parasites secrete metabolic products that promote the formation of free radicals, which in turn enhance the initiation of lipid peroxidation processes.

scholarly journals Evaluation of lipid peroxidation processes in patients with chronic parenteral viral hepatitis and HIV co-infection depending on degree of inflammatory process activity in the liver

2018 ◽  
Vol 90 (11) ◽  
pp. 37-43 ◽  
Author(s):  
L I Kolesnikova ◽  
M A Darenskaya ◽  
S I Kolesnikov ◽  
L A Grebenkina ◽  
M A Rashidova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Hiv Infection ◽  
Viral Hepatitis ◽  
Inflammatory Process ◽  
Chronic Viral Hepatitis ◽  
Reproductive Age ◽  
Inflammatory Activity ◽  
Control Group ◽  
Diene Conjugates ◽  
Peroxidation Processes

The aim of the study. To evaluate of parameters of lipid peroxidation - antioxidant defense changes in women of fertile age with chronic viral hepatitis (CVH) and with HIV (human immunodeficiency virus) infection, depending on the degree of inflammatory process activity in the liver. Materials and methods. 99 women of reproductive age were examined, of them 44 patients with chronic parenteral viral hepatitis, 27 patients with HIV co-infection (HIV + hepatitis B and/or C) and 28 practically healthy women (control group). Results. Patients with CVH in combination with HIV-infection with the presence of minimal and low degree of inflammatory activity in comparison with patients with CVH-monoinfection had a higher content of lipid peroxidation products (an increase in the content of diene conjugates and ketodienes and conjugated trienes), reduced values of total antioxidant activity, superoxide dismutase activity, as well as the concentration of fat-soluble vitamins α-tocopherol and retinol. In the group with CVH and HIV with moderate and high degree of activity, similar, but even more pronounced changes in the system of lipid peroxidation were observed. Conclusion. The presence of co-infection - CVH in combination with HIV infection with minimal and low, as well as moderate and high inflammatory activity, is characterized by more intensive lipid peroxidation processes and a pronounced lack of antioxidant factors than with CVH-monoinfection.

scholarly journals The state of oxidative-antioxidant homeostasis under the influence of subtoxic doses of sodium fluoride

2020 ◽  
Vol 10 (2) ◽  
pp. 5301-5305
Keyword(s):  
Antioxidant System ◽  
Sodium Fluoride ◽  
The State ◽  
Control Group ◽  
Blood Levels ◽  
Internal Organs ◽  
Diene Conjugates ◽  
Study Program ◽  
White Rats ◽  
Fluoride Intoxication

The objective of the study was to study the state of oxidative-antioxidant homeostasis under the conditions of the formation of experimental fluoride intoxication.24 white rats of the Wistar population were daily injected with sodium fluoride solution (20 mg/kg of animal weight). The continuation of intragastric xenobiotic intake was 1.5 months. The control group was monitored for 2 weeks toanalyse their condition. After 2 weeks, and The study program was conducted after 2 weeks when healthy mature (3 months) males weighing 180-200 grams were selected . At the end of the experiment, the control and experimental groups were clogged by decapitation. Evaluation of oxidative-antioxidant homeostasis was doneon the basis of the following indicators: the intensity of biochemiluminescence (BCL) of homogenates of internal organs and blood serum, the blood levels of SH-groups, glutathione, haptoglobin, diene conjugates (DC), malondialdehyde (MDA), cysteine, catalase activity, peroxidase, ceruloplasmine, glutathione peroxidase, superoxide dismutase. Vitamin C was determined in the adrenal glands. Analyzing the effect of fluoride intoxication on the state of oxidative and antioxidant system, it was shown that stimulation of free radical lipid peroxidation, the accumulation of peroxides, hydroperoxides, free radicals and inhibition of the antioxidant system are characteristic in this process. The obtained results confirm that all these mechanisms underlie the formation of structural and metabolic mechanisms of the pathogenesis of fluoride intoxication.

scholarly journals Study of prooxidant activity and some indicators of the pituitary-gonadal system in patients suffering from chronic endometritis

2020 ◽  
Vol 22 (1) ◽  
pp. 66-69
Author(s):  
N V Kirillova ◽  
O M Spasenkova ◽  
A G Platonova
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Protein Modification ◽  
Aliphatic Aldehyde ◽  
Oxidative Modification ◽  
Control Group ◽  
Diene Conjugates ◽  
Oxidative Modification Of Proteins ◽  
Chronic Endometritis ◽  
Oxidative Protein Modification

The state of lipid peroxidation and the oxidative modification of proteins in the blood of 29 women with chronic endometritis aged 27 to 40 years were studied. The control group consisted of 19 healthy women aged 27 to 44 years. The intensity of lipid peroxidation was estimated by the level of diene conjugates and malondialdehyde, the oxidative modification of proteins - by the level of carbonyl derivatives. It was shown that in the blood of women with chronic endometritis, the concentration of aliphatic aldehyde groups and 1,9 times the carbonyl groups in protein molecules increases by 3,3 times compared with the control group. A significant increase in diene conjugates by 34% and malondialdehyde in blood plasma and red blood cells by 47 and 43%, respectively, was established. The increase in lipid peroxidation products and oxidative protein modification in the blood indicates the development of oxidative stress in women suffering from chronic endometritis. Compared with healthy patients with chronic endometritis, a convincing tendency toward an increase in prolactin and anti- Muller hormone against a decrease in progesterone was revealed in the group compared to healthy ones. To predict possible complications in patients with chronic endometritis, it is advisable to study the indicators of protein and lipid peroxidation as markers of oxidative stress and the severity of the inflammatory process. In addition, such patients should be advised to take antioxidant drugs in combination with the basic therapy.

scholarly journals Assessment of the state of lipid peroxidation and antioxidant system in children with nephrotic form of glomerulonephritis

2017 ◽  
Vol 98 (3) ◽  
pp. 370-375
Author(s):  
R O Beglyarov
Keyword(s):  
Lipid Peroxidation ◽  
Nephrotic Syndrome ◽  
Blood Plasma ◽  
Antioxidant System ◽  
Malonic Dialdehyde ◽  
Control Group ◽  
Chronic Glomerulonephritis ◽  
Diene Conjugates ◽  
Control Value ◽  
In Blood Plasma

Aim. Study of the activity of lipid peroxidation and state of antioxidant system in children with a nephrotic form of chronic glomerulonephritis. Methods. 104 children with a nephrotic form of glomerulonephritis were examined. The average age of children was 10.18±4.03 years. 46.2% of children were in remission, 32.7% of children had 1st degree of activity, 14.4% had 2nd degree, and 6.7% had 3d degree of nephrotic syndrome. Control group included 30 children without chronic glomerulonephritis. The levels of diene conjugates, malonic dialdehyde, catalase, reduced glutathione, glutathione peroxidase, and glutathione reductase in blood plasma and erythrocytes were determined. Results. In children with chronic glomerulonephritis the concentration of diene conjugates and malonic dialdehyde was elevated in blood plasma and erythrocytes. In erythrocytes their concentration was statistically significantly higher at all degrees of activity of nephrotic syndrome than in control group. The level of catalase in patients’ blood plasma in comparison with the control value was reduced by 16.6%, in erythrocyte mass - by 25.9% (p

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