scholarly journals Chromato-mass-spectrometric assessment of volatile components for nettle (Urtica dioica L.)

2018 ◽  
pp. 73-77
Author(s):  
G. P. Smoylovskaya
Keyword(s):  
Urtica Dioica ◽  
Mass Spectrometric ◽  
Raw Material ◽  
Quantitative Composition ◽  
Volatile Components ◽  
Caryophyllene Oxide ◽  
Complex Action ◽  
Qualitative And Quantitative ◽  
Biologic Activity ◽  
Mass Spectrometric Detector

The species of nettle (Urtica dioica) are used long ago as hemostaticum for hemorrhages of various genesis both in non-traditional and in official medicine. Besides, the extractions of nettle possess hypolipidemic, cholagogue, anti-inflammatory, spasmolytic, prostatoprotective, adaptogenic properties, etc. Biologic activity of the nettle (Urtica dioica) is conditioned by the presence of various vitamins, carotenoids, proteins, flavonoids, hydroxycinnamic and organic acids, β-cytosterol, volatile compounds and other components. The aim of our study was to investigate the qualitative and quantitative composition of the volatile content of nettle. The research has been done on aero-dried vegetable raw-material Urtica dioica L. (leaves), stocked in June 2014 in Zaporizhzhya region. Identification and assessment of a number for volatile components has been done on the chromatograph Agilent Technology 6890 N with mass-spectrometric detector 5973. Approximately 20 components have been revealed in the leaves of Urtica dioica: hexahydrofarnesilacetate (165,19 mc/kg) and squalene (99,59 mc/kg) are predominated. Phytol, 2-methoxy-4-vinylphenol, nonacosane, etc. are presented in lesser number. The content of chrysanthenone, p-cymen-8-ol, verbenone, nerolidol, caryophyllene oxide, tricosane, tetracosane, heptacosane and untriacontane was insignificant (1,9% of the total amount of volatile components). Total number of volatile components in the leaves of Urtica dioica was 498,61 mc/mg. The research of qualitative composition and a number of volatile components in the leaves of Urtica dioica growing in Ukraine demonstrate expediency to carry out the further researches on the species Urtica dioica for using them in elaborating phytodrugs of complex action.

scholarly journals COPMONENT COMPOSITION OF ROOT IN TWO SPECIES OPLOPANAX (ARALIACEAE)

2019 ◽  
pp. 233-242
Author(s):  
Yelizaveta Sergeyevna Zhestovskaya ◽  
Sergey Valer'yevich Vasilevskiy ◽  
Aleksey Vadimovich Aksenov ◽  
Viktor Fedorovich Taranchenko ◽  
Andrey Nikolayevich Stavrianidi ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Comparative Analysis ◽  
Polar Compounds ◽  
Mass Spectrometric ◽  
Raw Material ◽  
Quantitative Composition ◽  
Root Extracts ◽  
Qualitative And Quantitative ◽  
Trimethylsilyl Derivatives ◽  
Chromatographic Peaks

The qualitative and quantitative composition of two species of Oplopanax root was studied by gas chromatography with mass spectrometric detection. Extraction of components from the investigated objects was carried out by extraction of dry ground raw material with 70% ethanol. For the analysis of polar compounds, the extracts were further derivatized to give the corresponding trimethylsilyl derivatives. Identification of components was carried out using commercial (NIST17, Wiley14) and own custom mass-spectrometer libraries. The percentage of components found was calculated using the areas of the corresponding chromatographic peaks. Found in these samples were 130 compounds of various classes: terpenes and their derivatives, alcohols, aldehydes, polyyns, polyenes, various acids and their derivatives, phenols, sterols, lignans, vitamins, nucleosides, glycosides and sugars. It has been established that the qualitative and quantitative composition of root extracts varies even within the same species. The presence of 26 compounds common to the two species of Oplopanax, including 12 acids, 3 polyyns, 6 terpenes and one representative each from the class polyenes, aldehydes, lignans, glycosides and sterols, was revealed. As a result of the comparative analysis, it was established that 4 compounds from the class of polyyns (falcarindiol acetate, oplopanediol acetate, oplopantriol A and oplopantriol B) are found only in the roots of O. horridus.

VOLATILE COMPONENTS FORMATION FEATURES OF FROZEN CHERRY WINE

2021 ◽  
Author(s):  
Олеся Сергеевна Егорова ◽  
Диляра Рамилевна Акбулатова
Keyword(s):  
Room Temperature ◽  
The Other ◽  
Quantitative Composition ◽  
Volatile Components ◽  
Qualitative And Quantitative

В работе представлены результаты исследований качественного и количественного состава летучих компонентов виноматериалов, полученных из свежих и замороженных плодов вишни. Отмечено, что дефростация плодов вишни при комнатной температуре привела к накоплению повышенных концентраций метанола и ацетальдегида в виноматериалах. Различия в концентрациях остальных исследуемых показателей были незначительными. The paper presents studies results of the the qualitative and quantitative composition of wine materials volatile components obtained from fresh and frozen cherry fruits. It is noted, that the cherry fruits defrosting at room temperature led to the accumulation of increased concentrations of methanol and acetaldehyde in wine materials. Differences in the concentrations of the other studied parameters were insignificant.

scholarly journals Development, Validation, and Application of the UPLC-DAD Methodology for the Evaluation of the Qualitative and Quantitative Composition of Phenolic Compounds in the Fruit of American Cranberry (Vaccinium macrocarpon Aiton)

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 467
Author(s):  
Rima Urbstaite ◽  
Lina Raudone ◽  
Mindaugas Liaudanskas ◽  
Valdimaras Janulis
Keyword(s):  
Phenolic Compounds ◽  
Biological Effects ◽  
Cost Effective ◽  
Vaccinium Macrocarpon ◽  
Raw Material ◽  
Quantitative Composition ◽  
Quercetin Derivatives ◽  
Qualitative And Quantitative ◽  
American Cranberry ◽  
Fruit Samples

Phenolic compounds in the fruit of American cranberry (Vaccinium macrocarpon Aiton) determine the antioxidant, anti-inflammatory, anticancer, and other biological effects. The berries are used in the production of medicinal preparations and food supplements, which highlights the importance of qualitative and quantitative analysis of phenolic compounds in cranberry fruit raw material. The aim of our study was to develop and validate an efficient, cost-effective, reproducible, and fast UPLC-DAD methodology for the evaluation of the qualitative and quantitative composition of phenolic compounds in raw material and preparations of American cranberry fruit. During the development of the methodology, chlorogenic acid and the following flavonols were identified in cranberry fruit samples: myricetin-3-galactoside, quercetin-3-galactoside, quercetin-3-glucoside, quercetin-3-α-L-arabinopyranoside, quercetin-3-α-L-arabinofuranoside, quercetin-3-rhamnoside, myricetin, and quercetin. The developed and optimized UPLC-DAD methodology was validated according to the guidelines of the International Council for Harmonization (ICH), evaluating the following parameters: range, specificity, linearity (R2 > 0.999), precision (%RSD < 2%), LOD (0.38–1.01 µg/mL), LOQ (0.54–3.06 µg/mL), and recovery (80–110%). The developed methodology was applied to evaluate the qualitative and quantitative composition of phenolic compounds in fruit samples of cranberry cultivars ‘Baifay’, ‘Bergman’, ‘Prolific’, and ‘Searles’, as well as ‘Bain-MC’ and ‘BL-12′ clones. In the tested samples, the majority (about 70%) of the identified flavonols were quercetin derivatives. The greatest amount of quercetin-3-galactoside (1035.35 ± 4.26 µg/g DW) was found in fruit samples of the ‘Searles’ cultivar, and the greatest amount of myricetin-3-galactoside (940.06 ± 24.91 µg/g DW) was detected in fruit samples of the ‘Woolman’ cultivar.

scholarly journals Adapting of a Method for Qualitative and Quantitative Determination of Squalene in Distillation Cuts of Sunflower Oil

2021 ◽  
Vol 32 ◽  
pp. 03007
Author(s):  
Irina Dubrovskaya ◽  
Evgeniy Gerasimenko ◽  
Margarita Slobodyanik ◽  
Sergey Sonin
Keyword(s):  
Sample Preparation ◽  
Quantitative Determination ◽  
Sunflower Oil ◽  
Integrated Approach ◽  
Raw Material ◽  
List Type ◽  
Mass Spectral ◽  
Qualitative And Quantitative ◽  
Mass Spectrometric Detector

Squalene is a naturally-occurring dihydro-triterpene hydrocarbon (C30H50) with six double bonds, which is an intermediate in the biosynthesis of phytosterol or cholesterol in plants or animals. The sources of squalene and the main methods forsqualene production and determination are consideredin brief. Sunflower oil distillation cuts have been selected as the subject of the study, since they area promising secondary raw material for the industrial squalene production. The methods of sample preparation and quantification of squalene in sunflower oil distillation cuts applying gas chromatography in combination with mass spectrometry have been adapted. The aim of the studyis to create an integrated approach to determining the qualitative and quantitative content of squalene in distillation cuts of vegetable oils. To achieve the goal of the study, the following tasks have been solved: – Amethod of sample preparation of distillation cuts for determination of squalene has been adapted; – A method of qualitative and quantitative determination of squalene in distillation cuts has been modified. As a result of this study, a technique for sample preparation of distillation cuts was proposed as well as a method for the qualitative and quantitative (absolute calibrationmethod) determination of squalene in distillation cuts of sunflower oil. To implement the technique, a Kristall 5000 gas chromatograph equipped with a mass spectrometric detector was used. Squalene and background components were recorded using the NIST 11 mass spectral database.

scholarly journals Research of fatty and organic acids in Rhododendron luteum Sweet leaves

2021 ◽  
pp. 63-70
Author(s):  
Т. М. Гонтова ◽  
В. П. Гапоненко ◽  
В. В. Машталер ◽  
О. С. Мала ◽  
М. А. Кулагіна
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Organic Acids ◽  
Saturated Fatty Acids ◽  
Mass Spectrometric ◽  
Raw Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Quantitative Content ◽  
Mass Spectrometric Detector ◽  
Derivatives Of

Rhododendron luteum Sweet of the Heath family Ericaceae is found on the territory of Ukraine both in the wild and is widely cultivated in botanical gardens, parks, squares. Leaves and inflorescences are used to treat heart disease, rheumatism, gout, and disorders of the nervous system. The literature data on the chemical composition relate to the presence of aminoacids, organic acids, essential oils, phenolic and triterpene compounds, andromedol derivatives. The aim of the work was to determine the composition and content of fatty and organic acids in the Rhododendron luteum Sweet leaves by gas chromatography-mass spectrometry. The object of the study was the leaves of Rhododendron luteum Sweet, collected on the territory of the botanical garden of H. S. Skovoroda Kharkiv National Pedagogical University in 2019. Gas chromatography - mass spectrometric study of the component composition and content of fatty and organic acids in the leaves of the studied plant on an Agilent Technologies 6890 chromatograph with a 5973 mass spectrometric detector was carried out. In Rhododendron luteum Sweet leaves 37 substances were found and identified. Fatty acids were represented by 17 compounds. Among saturated fatty acids, palmitic acid (3 276.2 mg/kg) was prevailed, among monounsaturated – oleic (736.78 mg/kg), among polyunsaturated – linolenic (1 617.65 mg/kg). The smallest amounts contained heneukocylic (46.33 mg/kg), lauric (58.17 mg/kg) and caproic (68.17 mg/kg) acids, which belong to saturated fatty acids. The presence of 20 organic acids in the raw material was also established: citric (8 680.30 mg/kg) and iso-citric (4 106.85 mg/kg) acids dominated in terms of quantitative content; oxalic (1 685.65 mg/kg) and malic (1 310.82 mg/kg) acids were contained in significant amounts. Among the derivatives of hydroxybenzoic acid, the greatest amount was presented by gentisic (187.76 mg/kg) acid, among the derivatives of hydroxycinnamic acids – p-coumaric (312.62 mg/kg). For the first time, the qualitative composition and quantitative content of fatty and organic acids in Rhododendron luteum Sweet leaves was studied by gas chromatography with a mass spectrometric detector. The obtained results indicate the prospects for further study of raw materials to develop drugs with membrane stabilizing and anti-inflammatory activity.

scholarly journals The Aerial Parts of Agrimonia procera Wallr. and Agrimonia eupatoria L. as a Source of Polyphenols, and Especially Agrimoniin and Flavonoids

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7706
Author(s):  
Elżbieta Karlińska ◽  
Beata Romanowska ◽  
Monika Kosmala
Keyword(s):  
Dry Matter ◽  
Raw Materials ◽  
Raw Material ◽  
Flavonoid Glycosides ◽  
Quantitative Composition ◽  
Food Ingredients ◽  
Qualitative And Quantitative ◽  
Aerial Parts ◽  
Current Trends ◽  
Polyphenolic Content

Plants of the genus Agrimonia L. perfectly fit the current trends in nutrition and food technology, namely, the need for raw materials with a high content of bioactive natural compounds, including polyphenols, which could be added to food. The composition of polyphenolics, including agrimoniin and flavonoids, in the aerial parts of Agrimonia procera Wallr. (A. procera) and Agrimonia eupatoria L. (A. eupatoria) (Rosaceae) was determined using HPLC-DAD-MS. The polyphenolic content of A. procera was found to be 3.9%, 3.2%, 2.9%, 1.8% and 1.1%, and that of A. eupatoria was determined to be 1.3%, 0.3%, 0.9%, 0.6% and 0.5% in the dry matter of leaves, stems, fruits, seeds and hypanthia, respectively. Except for A. procera hypanthia, agrimoniin was the main polyphenolic compound in the aerial parts of the studied Agrimonia species. Both plants are also a valuable source of flavonoid glycosides, especially apigenin, luteolin and quercetin. The obtained data indicate that both A. procera and A. eupatoria are potentially good sources of polyphenols (albeit significantly different in terms of their qualitative and quantitative composition), and may not only be a medicinal raw material, but also a valuable material for food use such as nutraceuticals or functional food ingredients.

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