scholarly journals Extraction, isolation and characterization of depsidones from Usnea baileyi (Stirt.) Zahlbr collected from tree barks in Tam Bo Mountain of Di Linh, Lam Dong Province, Viet Nam

2018 ◽  
Vol 21 (1) ◽  
pp. 24-31
Author(s):  
Kieu Van Nguyen ◽  
Huy Thuc Duong
Keyword(s):  
Spectroscopic Analysis ◽  
Normal Phase ◽  
Acetone Extract ◽  
Viet Nam ◽  
Current Investigation ◽  
Chemical Structures ◽  
Isolation And Characterization ◽  
Tree Barks ◽  
Layer Chromatography

Chemical data on the lichen Usnea baileyi are scarce. The current investigation reports the extraction, isolation and characterization of depsidones from this lichen collected in Lam Dong Province. Eight depsidones were isolated and elucidated, including methylstictic acid (1), stictic acid (2), cryptostictic acid (3), 8’-O-methylmenegazziaic acid (4), menegazziaic acid (5), 8’- O-methylconstictic acid (6), constictic acid (7), and hypoconstictic acid (8). Multi chromatographic methods comprising normal phase silica gel column chromatography and thin layer chromatography were applied to the crude acetone extract to isolate and purify compounds 1-8. Their chemical structures were elucidated by spectroscopic analysis as well as comparing their data with ones in the literature.

scholarly journals Chemical constituents of Euphorbia tirucalli L.

2019 ◽  
Vol 2 (5) ◽  
pp. 76-82
Author(s):  
Dung Thi Kim Le ◽  
Hao Xuan Bui ◽  
Tuyet Thi Anh Nguyen ◽  
Tuyen Nguyen Kim Pham ◽  
Huy Thuc Duong
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Spectroscopic Analysis ◽  
Chemical Constituents ◽  
Normal Phase ◽  
Chemical Structures ◽  
Euphorbia Tirucalli ◽  
Arjunolic Acid ◽  
First Time ◽  
Layer Chromatography

Euphorbia tirucalli has not been chemically studied much in Vietnam. This research described the isolation and elucidation of compounds isolated from the plant collected in Binh Thuan. Multiple chromatographic methods were applied, including normal phase silica gel column chromatography and thin-layer chromatography. Seven compounds were isolated and their chemical structures were elucidated by spectroscopic analysis as well as comparing their data with the ones in the literature. They are arjunolic acid (1), eriodictyol (2), quercitrin (3), afzelin (4), scopoletin (5), 3,3′,4- trimethylellagic acid (6), and gallic acid (7). Among them, compound 1 a major component was isolated for the first time in Euphorbia genus, while three compounds 2, 4, and 5 were isolated from this species for the first time.

scholarly journals Chemical Derivatization and Characterization of Novel Antitrypanosomals for African Trypanosomiasis

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4488
Author(s):  
Aboagye Kwarteng Dofuor ◽  
Temitayo Samson Ademolue ◽  
Cynthia Mmalebna Amisigo ◽  
Kwaku Kyeremeh ◽  
Theresa Manful Gwira
Keyword(s):  
Structural Modification ◽  
Spectroscopic Analysis ◽  
Microscopic Analysis ◽  
The Novel ◽  
Chemical Derivatization ◽  
African Trypanosomiasis ◽  
Chemical Structures ◽  
Normal Ratio

The search for novel antitrypanosomals and the investigation into their mode of action remain crucial due to the toxicity and resistance of commercially available antitrypanosomal drugs. In this study, two novel antitrypanosomals, tortodofuordioxamide (compound 2) and tortodofuorpyramide (compound 3), were chemically derived from the natural N-alkylamide tortozanthoxylamide (compound 1) through structural modification. The chemical structures of these compounds were confirmed through spectrometric and spectroscopic analysis, and their in vitro efficacy and possible mechanisms of action were, subsequently, investigated in Trypanosoma brucei (T. brucei), one of the causative species of African trypanosomiasis (AT). The novel compounds 2 and 3 displayed significant antitrypanosomal potencies in terms of half-maximal effective concentrations (EC50) and selectivity indices (SI) (compound 1, EC50 = 7.3 μM, SI = 29.5; compound 2, EC50 = 3.2 μM, SI = 91.3; compound 3, EC50 = 4.5 μM, SI = 69.9). Microscopic analysis indicated that at the EC50 values, the compounds resulted in the coiling and clumping of parasite subpopulations without significantly affecting the normal ratio of nuclei to kinetoplasts. In contrast to the animal antitrypanosomal drug diminazene, compounds 1, 2 and 3 exhibited antioxidant absorbance properties comparable to the standard antioxidant Trolox (Trolox, 0.11 A; diminazene, 0.50 A; compound 1, 0.10 A; compound 2, 0.09 A; compound 3, 0.11 A). The analysis of growth kinetics suggested that the compounds exhibited a relatively gradual but consistent growth inhibition of T. brucei at different concentrations. The results suggest that further pharmacological optimization of compounds 2 and 3 may facilitate their development into novel AT chemotherapy.

scholarly journals Lagerindicine, a New Pyrrole Alkaloid Isolated from the Flowers of Lagerstroemia indica Linnaeus

2020 ◽  
Author(s):  
Yi Chen ◽  
Song-Wei Li ◽  
Fang-Zhou Yin ◽  
Min Yang ◽  
Xia-Juan Huan ◽  
...  
Keyword(s):  
Total Synthesis ◽  
Spectroscopic Analysis ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Cytotoxic Effects ◽  
Etoh Extract ◽  
Isolation And Characterization ◽  
Phytochemical Investigation ◽  
Hct 116

Abstract A phytochemical investigation of the EtOH extract of the flowers of Lagerstroemia indica L. led to the isolation and characterization of a new pyrrole alkaloid, named lagerindicine (1), along with four known compounds (2–5). Their structures were elucidated by the detailed spectroscopic analysis and comparison with literature data, whereas the structure, in particularly, the absolute configuration (AC) of 1, was firmly determined by total synthesis. All the isolates were evaluated for their cytotoxic effects against human colon cancer cell (HCT-116), and compound 3 exhibited weak cytotoxicity with IC50 value of 28.4 μM. Graphic Abstract

scholarly journals Isolation and Characterization of New Anti-Inflammatory and Antioxidant Components from Deep Marine-Derived Fungus Myrothecium sp. Bzo-l062

Marine Drugs ◽  
2020 ◽  
Vol 18 (12) ◽  
pp. 597
Author(s):  
Xiaojie Lu ◽  
Junjie He ◽  
Yanhua Wu ◽  
Na Du ◽  
Xiaofan Li ◽  
...  
Keyword(s):  
Deep Sea ◽  
High Performance ◽  
Oxygen Radical ◽  
Normal Phase ◽  
Oxide Formation ◽  
Nitric Oxide Formation ◽  
Isolation And Characterization ◽  
Anti Inflammatory ◽  
New Compounds

In the present study, four new compounds including a pair of 2-benzoyl tetrahydrofuran enantiomers, namely, (−)-1S-myrothecol (1a) and (+)-1R-myrothecol (1b), a methoxy-myrothecol racemate (2), and an azaphilone derivative, myrothin (3), were isolated along with four known compounds (4–7) from cultures of the deep-sea fungus Myrothecium sp. BZO-L062. Enantiomeric compounds 1a and 1b were separated through normal-phase chiral high-performance liquid chromatography. The absolute configurations of 1a, 1b, and 3 were assigned by ECD spectra. Among them, the new compound 1a and its enantiomer 1b exhibited anti-inflammatory activity, inhibited nitric oxide formation in lipopolysaccharide-treated RAW264.7 cells, and exhibited antioxidant activity in the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and oxygen radical absorbance capacity assays.

A Yellow Discoloration of Cooked Cured Meat Products - Isolation and Characterization of the Causative Organism

1989 ◽  
Vol 52 (6) ◽  
pp. 392-395 ◽  
Author(s):  
ALLAN M. WHITELEY ◽  
MARIETTE D. D'SOUZA
Keyword(s):  
Meat Products ◽  
Heat Processing ◽  
Causative Organism ◽  
Refrigerated Storage ◽  
Anaerobic Conditions ◽  
Isolation And Characterization ◽  
Cured Meat ◽  
Layer Chromatography ◽  
Aerobic And Anaerobic

An organism has been isolated in pure culture that is capable of causing a yellow discoloration on a cooked cured meat substrate under aerobic and anaerobic conditions. This organism has subsequently been identified as a member of the genus Streptococcus. The characteristics were such that a tentative identification as Streptococcus faecium, sub-species casseliflavus was made. The organism is extremely heat resistant and could survive a heat processing of 71.1°C for 20 min. The yellow discoloration on a vacuum packaged luncheon style meat takes 3 to 4 wk to become apparent under refrigerated storage. The pigment was extracted and separated by thin layer chromatography (TLC). The evidence points to the compound being carotenoid in nature.

Chemical constituents of two Ecuadorian medicinal plants, Tagetes terniflora Kunth and Croton rivinifolius Kunth

2021 ◽  
Author(s):  
Alírica I Suárez ◽  
Vladimir Morocho ◽  
Vladimir Luna ◽  
Katty Castillo ◽  
Chabaco Armijos
Keyword(s):  
Medicinal Plants ◽  
Spectroscopic Analysis ◽  
Chemical Constituents ◽  
Two Dimensional ◽  
Phytochemical Study ◽  
Isolation And Characterization ◽  
Organic Extracts ◽  
Medicinal Properties ◽  
Nmr Methods

Phytochemical study of two medicinal plants from Ecuador, Tagetes terniflora Kunth, and Croton rivinifolius Kunth, led to the isolation and characterization of the major constituents present in the organic extracts obtained from these plants: 5-(4-acetoxy-1-butynil)-2,2’-bi-thiophene (1), 5-methyl-2,2’:5’,2”- terthiophene (2), patuletin (3) from Tagetes terniflora, and isocorydine (4), sweroside (5), tiliroside (6) from Croton rivinifolius. The structures of these compounds were established by spectroscopic analysis including two-dimensional NMR methods, MS, and comparison with published spectral data. They are recognized as secondary metabolites that represent the chemotaxonomy of Tagetes and Croton genera and could be responsible for the recognized medicinal properties attributed to these species. This paper deals with the first report that shows the presence of these compounds in these plants.

Potent inhibitors of glucagon-stimulated adenylate cyclase associated with serum lipoprotein particles

1989 ◽  
Vol 67 (11-12) ◽  
pp. 759-762
Author(s):  
Philip J. Bilan ◽  
Jonathan B. Weitzman ◽  
Tracey Pflieger ◽  
C. Roy D. Lancaster ◽  
Alan Stafford ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Adenylate Cyclase ◽  
Serum Lipoprotein ◽  
Normal Phase ◽  
Acetone Extract ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Inhibitory Effects ◽  
Acetone Extraction ◽  
Layer Chromatography

Lipoprotein fractions from some individuals have inhibitory effects on rat liver adenylate cyclase. Precipitation of the lipoprotein fractions with acetone released an inhibitory factor, which was soluble in acetone–H2O (3:1, v/v). The inhibition was greater against glucagon-stimulated activity than against basal activity. Acetone extraction increased the potency of inhibition. All three lipoprotein fractions, i.e., very low, low, and high density lipoproteins, released some inhibitory component after acetone extraction. The inhibitor was concentrated in the lipoprotein fractions, since acetone extraction of plasma did not release an inhibitor. The acetone extract from the very low density liproprotein was the most inhibitory. This material was further purified and partially characterized. The inhibitor had a molecular mass of about 500. It was inhibitory at micromolar concentrations. The material was sufficiently hydrophobic to migrate in normal-phase thin-layer chromatography (TLC). Nuclear magnetic resonance results indicated that it was not a polar lipid. There were several different inhibitory factors that were separable by TLC. The sequestration of these inhibitors into lipoproteins reduced their effectiveness in inhibiting the action of counter-regulatory hormones, such as glucagon.Key words: glucagon, adenylate cyclase, lipoprotein, diabetes mellitus.

Sign in / Sign up

Export Citation Format

Share Document