scholarly journals Analysis of the geochemical, microbiological state and floral composition of springs in Aktobe, West Kazakhstan and Atyrau regions

2020 ◽  
Vol 130 (1) ◽  
pp. 88-102
Author(s):  
K.M. Akhmedenov ◽  
◽  
G.Z. Idrisova ◽  
N.Kh. Sergaliev ◽  
◽  
...  
Keyword(s):  
Bacterial Contamination ◽  
Total Coliform ◽  
Spring Water ◽  
Coliform Bacteria ◽  
Protected Species ◽  
Western Kazakhstan ◽  
Microbial Number ◽  
West Kazakhstan ◽  
Total Coliform Bacteria ◽  
Microbiological Research

The results of hydrochemical and Toxicological examination of 16 springs in Aktobe, 12 springs in West Kazakhstan and three springs in Atyrau regions of Western Kazakhstan are presented. The characteristics of the content of cations and anions, heavy metals in hydrogeochemical samples of spring water, indicators of microbiological research of water (total microbial number, total coliform bacteria, thermotolerant coliform bacteria), factors of bacterial contamination of springs in comparison with sanitary standards and water quality requirements are given. The results of floral studies of springs, a list of rare and protected species of flora of spring complexes listed in the Red Book of Russia and Kazakhstan are presented

Pengaruh Suhu Penyimpanan terhadap Organoleptik, Derajat Keasaman dan Pertumbuhan Bakteri Coliform pada Susu Pasteurisasi

2016 ◽  
Vol 10 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Agustina Arianita Cahyaningtyas ◽  
Wiwik Pudjiastuti ◽  
Ilham Ramdhan
Keyword(s):  
Storage Temperature ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Pasteurized Milk ◽  
Initial Analysis ◽  
Total Plate Count ◽  
Plate Count Method ◽  
Pathogenic Microbes ◽  
Total Coliform Bacteria

One attempt to reduce the number of pathogenic microbes in milk is through the pasteurization process. This research aims to determine the effect of storage temperature on the organoleptic, acidity (pH) and growth of coliform bacteria in pasteurized milk. Pasteurized milk is stored at the varies of temperature  4°C (observed for 14 days), 10°C-15°C (observed for 14 days) and 25°C-27°C (observed for 22 hours), as well as also conducted an initial analysis pasteurized milk. The parameters were observed among other organoleptic (smell, taste, color, texture), pH and total coliform bacteria. Testing acidity using pH paper, while the growth of coliform bacteria testing done using Total Plate Count method based on ISO 2897 in 2008. The results of this study indicate that storage at 4°C for 14 days, organoleptic pasteurized milk is still good until the day ke- 8, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 3100x101 CFU / ml. Storage at 10°C-15°C for 14 days, organoleptic pasteurized milk is still good until the 6th day, the pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 5729x101 CFU / ml. Storage at 25°C-27°C for 22 days, organoleptic pasteurized milk is still good until the 9th, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 4.3 x106 CFU / ml.ABSTRAKSalah satu usaha untuk mengurangi jumlah mikroba patogen pada susu adalah melalui proses pasteurisasi. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan terhadap organoleptik, derajat keasaman (pH) dan pertumbuhan bakteri Coliform pada susu pasteurisasi. Susu pasteurisasi disimpan pada suhu yang bervariasi yaitu suhu 4°C (diamati selama 14 hari), suhu 10°C-15°C (diamati selama 14 hari) dan suhu 25°C-27°C (diamati selama 22 jam), serta dilakukan pula analisa awal susu pasteurisasi. Parameter yang diamati antara lain organoleptik (bau, rasa, warna, tekstur), pH dan jumlah bakteri Coliform. Pengujian derajat keasaman menggunakan kertas pH, sedangkan pengujian pertumbuhan bakteri Coliform dilakukan dengan menggunakan metode Total Plate Count berdasarkan SNI 2897 Tahun 2008. Hasil penelitian ini menunjukkan bahwa penyimpanan pada suhu 4°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai dengan hari ke-8, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 3100x101 Cfu/ml. Penyimpanan pada suhu 10°C-15°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai hari ke-6, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 5729x101 Cfu/ml. Penyimpanan pada suhu 25°C-27°C selama 22 hari, organoleptik susu pasteurisasi masih baik sampai jam ke-9, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 4,3 x106 Cfu/ml.Kata kunci : bakteri coliform, derajat keasaman, suhu penyimpanan, organoleptik, susu pasteurisasi

scholarly journals Analysis of Water Quality and River Waters Microbology for Manifestation of Food Safety

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Rizky Muliani Dwi Ujianti ◽  
Althesa Androva
Keyword(s):  
Water Quality ◽  
Food Security ◽  
Quality Management ◽  
Water Quality Management ◽  
Quality Standard ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Domestic Waste ◽  
Total Coliform Bacteria

 Abstract. Banjir Kanal Barat is a river in the Garang watershed, Semarang City, Central Java, Indonesia. Its function is as a source of water for the community. The level of pollution in this river is already high. The purpose of this study is to provide advice to governments, communities and related stakeholders to realize integrated river management, and fisheries-based food security is achieved. This research method is: analyzing the water quality of the Banjir Kanal Barat river, and analyzing the amount of faecal and total coliform bacteria content in the Banjir Kanal Barat river, and analyzing how to overcome the decline in the quality of waters of the Banjir Kanal Barat river due to faecal and total coliform bacteria pollution. The results showed that the water quality at the research location was still in the quality standard. The content of coliform dan faecal bacteria at the study site exceeds the quality standard, this is due to the influence of domestic waste from households. The thing that needs to be done is counseling the existence of a clean and healthy life, especially for people who are still throwing domestic waste into the river. The existence of water purification equipment is also very necessary to overcome this problem. Water quality management can be done with policy analysis. Regulations related to water quality management can be analyzed and then given solutions and recommendations related to these rules so that policies can be taken that are sustainable, integrated, and coordinated between various parties in managing river water quality and food security. Keywords: food security, water quality, river, faecal coliform, total coliform

scholarly journals ANALISIS KELIMPAHAN TOTAL BAKTERI COLIFORM DI PERAIRAN MUARA SUNGAI SAYUNG, MOROSARI, DEMAK (Analysis of Total Abundance of Coliform Bacteria at the Sayung River Estuary, Morosari, Demak)

2018 ◽  
Vol 14 (1) ◽  
pp. 30
Author(s):  
Lani Febriana Safitri ◽  
Niniek Widyorini ◽  
Oktavianto Eko Jati
Keyword(s):  
Water Quality ◽  
Organic Matter ◽  
Dissolved Oxygen ◽  
Random Sampling ◽  
River Estuary ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Total Abundance ◽  
Average Abundance ◽  
Total Coliform Bacteria

Perairan Muara Sungai Sayung terletak di wilayah Kabupaten Demak sebelah barat. Perairan ini telah dipengaruhi oleh berbagai aktivitas manusia yang menyebabkan terjadinya penurunan kualitas perairan. Bakteri Coliform merupakan bakteri gram negatif yang berbentuk batang dan tidak membentuk spora. Bakteri ini merupakan bakteri indikator terjadinya pencemaran perairan maupun indikator keberadaan bakteri patogen lainnya. Perhitungan total bakteri Coliform perlu dilakukan untuk mengetahui adanya pencemaran limbah organik yang terjadi di Perairan Muara Sungai Sayung, Morosari, Demak. Tujuan dari penelitian ini adalah untuk mengetahui kelimpahan total bakteri Coliform, dan status perairan di Perairan Muara Sungai Sayung, Morosari, Demak. Penelitian ini juga bertujuan untuk mengetahui hubungan dari jumlah total bakteri Coliform dengan bahan organik terlarut dan oksigen terlarut (DO). Pelaksanaan penelitian ini dilakukan pada bulan Mei 2018. Metode yang digunakan dalam penelitian ini adalah metode survei dan teknik pengambilan sampel menggunakan metode random sampling yang dilakukan pada lima stasiun. Hasil penelitian menunjukkan bahwa kelimpahan rata-rata total bakteri Coliform yang diperoleh berkisar antara 4 x 103 – 550 x 10 3/100ml. Kelimpahan rata-rata total bakteri Coliform yang diperoleh pada setiap stasiun menunjukkan bahwa kandungan total bakteri Coliform telah melebihi baku mutu air menurut Keputusan Menteri Lingkungan Hidup Nomor 51 Tahun 2004. Keberadaan total bakteri Coliform memiliki hubungan yang lemah dengan kandungan bahan organik terlarut, sedangkan keberadaan total bakteri Coliform memiliki hubungan yang cukup dengan oksigen terlarut (DO) di perairan Muara Sungai Sayung Morosari, Demak. Sayung River Estuary is located in the western part of Demak Regency. This estuary has been affected by various human activities which have led to a decline in water quality. Coliform bacteria are a gram-negative bacteria that are rod-shaped and do not form spores. This bacterium is an indicator bacteria of water pollution and an indicator of the presence of other pathogenic bacteria. Total Coliform bacteria calculation needs to be carrid out to find out if there any contamination of organic waste in Sayung River Estuary, Morosari, Demak.. The purpose of this research was to determine total abundance of Coliform bacteria, water quality in Sayung River Estuary, Morosari, Demak and relationship between the total abundance of Coliform bacteria with dissolved organic matter and dissolved oxygen (DO). This research was carried out in May 2018. The method that used in this research were survey method and the sampling technique was random sampling method in five stations. The results showed that the average abundance of total Coliform bacteria obtained ranged between 4 x 103 - 550 x 103 / 100ml. Average abundance of total Coliform bacteria obtained at each station shows that the total content of Coliform bacteria has exceeded the water quality standard according to Minister of Environment Decree number 51 of 2004. The total presence of Coliform bacteria has a weak relationship with the content of dissolved organic matter, while the total number of Coliform bacteria have sufficient relationship with dissolved oxygen (DO) in the Sayung River Estuary,Morosari, Demak.

scholarly journals Effects of Boiling Water Temperature on Biofilm Formation in PTI Community Potable Water

2015 ◽  
Vol 10 (1) ◽  
pp. 84-90
Author(s):  
E Fadairo ◽  
M Otite-Douglas
Keyword(s):  
Dissolved Oxygen ◽  
Water Sample ◽  
Distribution System ◽  
Biofilm Formation ◽  
Potable Water ◽  
Total Coliform ◽  
Boiling Water ◽  
Coliform Bacteria ◽  
Ph Values ◽  
Total Coliform Bacteria

This study investigated the effects of boiling temperature and associated physico-chemical parameters on the Petroleum Training Institute potable water and the possibility of biofilm formation in its delivery systems. A total of 25 potable water samples were used for this study. The environmental parameters investigated were pH, conductivity, total dissolved solids (TDS), total suspended solid (TSS), dissolved oxygen (DO1), / DO5, salinity, resistivity, total coliform bacteria (as an indicator of possible biofilm presence in the distribution system) and biofilm . An overall prevalence of <1 of the total coliform bacteria was observed in the plus-boiling and minus-boiling potable water sample, except for the female hostel which showed moderate stain for the qualitative biofilm test. For the minus-boiling water sample, pH values were between 5.04±0.47 to 6.82±0.48; Total suspended solids ranged between 0.09±0.05-0.17±0.02, total dissolved solid ranged between 4.07±0.73 to 5.58±0.70, conductivity values ranged between 8.02±0.90 to 11.54±1.67, dissolved oxygen ranged between 1.97±0.26 to 3.12 ±0.13, the DO5 ranged between 1.91±0.32 to 2.72± 0.29 while resistivity ranged between 7.79±0.13 to 10.88±0.18. Values for the Plus-boiling and filtered samples showed a pH range of 6.02±0.26 to 6.95±0.26; conductivity 7.21±0.10 to 9.88±0.67; DO ranged between 1.01±0.14 to 2.08±0.35, DO 5 was 1.02±0.02 to 2.01±0.38, TSS and TDS ranged between 0.02±0.001, 3.74±0.62 to 0.03±0.002 and 4.95±0.42 respectively while resistivity ranged between 1.02±0.11 to 1.98±0.16. For all parameters analyzed, values obtained falls within the WHO limit for potable water except for the qualitative biofilm test on FSH minus-boiling water sample which gave moderate stain with 0.1% crystal violet stain and the pH values which fall below WHO acceptable limits. Boiling and filtration of potable water irrespective of the source is campaigned from this study in order to enhance the prevention and management of water borne diseases within the institute community.

scholarly journals Household stored water quality in an intermittent water supply network in Panama

2020 ◽  
Vol 10 (2) ◽  
pp. 298-308
Author(s):  
Carlos I. Gonzalez ◽  
John Erickson ◽  
Karina A. Chavarría ◽  
Kara L. Nelson ◽  
Amador Goodridge
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Supply ◽  
Water Storage ◽  
Total Coliform ◽  
Drinking Water Supply ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Probable Number ◽  
Total Coliform Bacteria

Abstract Safe water storage is critical to preserve water quality, especially when intermittent piped drinking water supply creates a need for household storage. This study characterized household storage practices and stored water quality in 94 households (N = 94) among four peri-urban neighborhoods in Arraiján, Panama with varying degrees of supply intermittency. We found that 18 (19.1%) households stored drinking water in unsafe containers. Forty-four (47%) samples of household stored drinking water had residual chlorine levels &lt;0.2 mg/L. While 33 (35.1%) samples were positive for total coliform bacteria, only 23 (24.4%) had &gt;10 most probable number (MPN)/100 mL total coliform bacteria. Eight (44%) samples were positive for Escherichia coli, whereas only one (1.3%) sample from the safe containers was positive. Twenty-nine (30.9%) samples had &gt;500 MPN/mL heterotrophic plate count bacteria. These findings suggest that longer supply interruptions were associated with longer storage times and lower chlorine residual, which were associated with higher concentrations of indicator bacteria. This is one of the first studies in the Central-American region to show an association between the lack of turnover (replacement with fresh water) and greater contamination during household water storage. Thus, when drinking water supply is not completely continuous and household storage is required, decreasing the time between supply periods can facilitate safer water storage. Public awareness and education are also recommended to increase hygiene practices during water collection and storage.

ColiComplete® Substrate-Supporting Disc Method for Confirmed Detection of Total Coliforms and Escherichia coli in All Foods: Comparative Study

1994 ◽  
Vol 77 (1) ◽  
pp. 58-63 ◽  
Author(s):  
Philip T Feldsine ◽  
Maria T Falbo-Nelson ◽  
David L Hustead
Keyword(s):  
Escherichia Coli ◽  
Comparative Study ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Total Coliforms ◽  
E Coli ◽  
Total Coliform Bacteria ◽  
Better Than

Abstract The ColiComplete® substrate-supporting disc (SSD) method for simultaneous confirmed total coliform count and Escherichia coli determination in all foods was compared with the AOAC most probable number (MPN) methods 966.23 and 966.24. In this comparative study, 20 water and food types were analyzed; 7 of these foods were naturally contaminated with coliform bacteria, 6 food types were naturally contaminated with E. coli, and the remaining foods were inoculated with coliform bacteria and/or E. coli. Data were analyzed separately for total coliform bacteria and for E. coli. Mean log MPN counts were determined by the SSD method and the appropriate AOAC MPN procedure. Results were then analyzed for mean log MPN differences and variance, according to methods described by AOAC INTERNATIONAL Results for both total conforms and E. coli indicate that the SSD method is equivalent to or better than AOAC MPN methods 966.23 and 966.24.

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