scholarly journals Uji Fenolik Dan Toksisitas (Brine Shrimp Lethality Test) Ektrak Methanol Daun Benalu Duku Hijau (Dendrohtoe pentandara (L.) Miq) Dan Merah (Scurrula ferruginea (Jack) Danser)

2019 ◽  
Vol 2 (1) ◽  
pp. 84-87
Author(s):  
Rini Hardiyanti ◽  
Lamek Marpaung ◽  
I Ketut Adnyana ◽  
Partomuan Simanjuntak
Keyword(s):  
Phenolic Compounds ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Test Results ◽  
Cytotoxic Potential ◽  
Brine Shrimp Lethality ◽  
Phenolic Extract ◽  
Toxicity Effects

Uji fenolik ekstrak dilakukan dengan menggunakan FeCl 3 untuk mengetahui kandungan senyawa fenolik dari daun benalu duku hijau (Dendrophthoe pentandara (L.) Miq) dan daun benalu duku merah (Scurrula ferruginea (Jack) Danser) serta efek toksisitas telah diamati dengan metode Brine Shrimp Lethality Test (BSLT). Daun benalu duku diekstrak hingga diperoleh ekstrak methanol. Hasil pengujian ekstrak methanol menunjukkan bahwa ekstrak benalu duku hijau dan merah mempunyai potensi sitotoksik terhadap larva udang dengan nilai LC 50 masing-masing 13.95 ppm dan 22.37 ppm. Hasil tersebut menunjukkan bahwa ekstrak daun benalu duku hijau (Dendrophthoe pentandara (L.) Miq) bersifat lebih toksik dibandingkan ekstrak daun benalu duku merah (Scurrula ferruginea (Jack) Danser).   Phenolic extract test was carried out using FeCl 3 to determine the content of phenolic compounds from parasite leaves of green duku (Dendrophthoe pentandara (L.) Miq) and red duku parasitic leaves (Scurrula ferruginea (Jack) Danser) and the toxicity effects that have been observed using the Brine Shrimp Lethality Test (BSLT). The parasitic leaves were extracted to obtain methanol extract. The test results of methanol extract showed that the extracts of green and red duku parasites had cytotoxic potential against shrimp larvae with LC 50 values ​​of 13.95 ppm and 22.37 ppm respectively. These results indicated that the parasite leaves extract in green duku was more toxic than the extract of the red leaves parasite.

scholarly journals ANALISIS FITOKIMIA DAN PENENTUAN NILAI LC50 EKSTRAK METANOL DAUN LIWAS

2014 ◽  
Vol 14 (2) ◽  
pp. 125
Author(s):  
Gerry Sumihe ◽  
Max R. J. Runtuwene ◽  
Johnly A. Rorong
Keyword(s):  
Secondary Metabolites ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Test Results ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

ABSTRAK Penentuan nilai LC50 dari ekstrak metanol daun liwas menggunakan metode Brine Shrimp Lethality Test (BSLT) terhadap hewan indikator udang Artemia salina menunjukkan nilai LC50 dari  A. salina bersifat sangat toksik.Nilai LC50 yang didapatkan sebesar 15,696 mg/L. Nilai LC50 ditentukan dengan menggunakan metode SPSS 20.0.Ekstrak metanol daun liwas mengandung kandungan senyawa metabolit sekunder seperti flavonoid, tanin dan saponin sebagai hasil uji fitokimia. Kata kunci: BSLT, Daun Liwas, Metabolit Sekunder,LC50.   PHYTOCHEMICAL ANALYSIS AND DETERMINING LC50 VALUE OF LIWAS LEAVE METHANOL EXTRACT ABSTRACT Determination of LC50 of methanol extract of leaves liwas using Brine Shrimp Lethality Test (BSLT) against animal indicator shrimp Artemia salina showed LC50 values of  A. salina is highly toxic. LC50 values were obtained at 15.696 mg / L. LC50 values determined using SPSS 20.0. Liwas leaf methanol extract contains compounds secondary metabolites such as flavonoids, tannins and saponins as phytochemical test results. Keywords: BSLT, Leaves Liwas, Secondary Metabolites, LC50.

scholarly journals Steroids from the Leave of Persicaria perfoliata (L.) H. Gross

2019 ◽  
Vol 22 (2) ◽  
pp. 224-227
Author(s):  
Md Sagar Hossain ◽  
NM Mofiz Uddin Khan ◽  
Md Saiful Quddus ◽  
AM Sarwaruddin Chowdhury
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Persicaria Perfoliata ◽  
Test Organisms

β-sitosterol (1) and β-sitosterol-D-glucoside (2) were isolated from the dichloromethane soluble fraction of a methanol extract of the leave of Persicaria perfoliata. The crude fraction was subjected to antimicrobial screening and brine shrimp lethality bioassay, where moderate antimicrobial activity was observed against most of the test organisms was seen. The hexane soluble fraction also displayed significant cytotoxic activity with LC50 0.64 μg/ml in brine shrimp lethality bioassay. This is the first report of occurrence of these compounds from this plant. Bangladesh Pharmaceutical Journal 22(2): 224-227, 2019

scholarly journals Investigation of Antioxidant, In-vitro Cytotoxic, and In-vivo Antitumor Effects of Leaf Extracts of Annona Reticulata

2016 ◽  
Vol 29 (2) ◽  
pp. 70-74
Author(s):  
Shashwata Biswas ◽  
Mohammad Shahriar ◽  
Jahan Ara Khanam ◽  
Chowdhury Rafiqul Ahsan
Keyword(s):  
Petroleum Ether ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Control Group ◽  
Percentage Increase ◽  
Initial Weight ◽  
Antitumor Effects ◽  
Brine Shrimp Lethality ◽  
Annona Reticulata

This study was carried out to evaluate the antioxidant, brine shrimp lethality, bioassay and antitumor effects of nhexane, petroleum ether, methanol and chloroform extracts of Annona reticulata leaves. All extracts demonstrated good cupric reducing activity with increasing concentrations keeping methanol extract at the top and n-hexane extract at the second position. The methanol extract was found to have the highest antioxidant capacity 28±26.88 followed by n-hexane 16.95±11.39 and chloroform 12.85±10.32 in the form of mg of ascorbic acid equivalent per gram of crude extract. The methanol extract also showed the highest values of 2.41±0.963 mg of gallic acid equivalent phenolic content and 6.54±4.08 mg of quercetin equivalent flavonoid content per g of crude extract. In the brine shrimp lethality bioassay, LC50 values of methanol, chloroform, petroleum ether, and n-hexane extracts were found to be 1.084?g/ml, 1.096?g/ml, 1.995?g/ml and 3.126 ?g/ml, respectively. These findings suggest that A. reticulata leaves can be a good source of antioxidants and possess significant cytotoxic effect. In a narrow spectrum investigation on antitumor activity of the extracts, we found that the methanol extract inhibited the tumor growing process (control group, chloroform group and petroleum ether group showed significant rise in body weight; i.e., 8% - 14% of initial weight compared to that of methanol group showing a rise of 0.45% - 0.94% of initial weight) and improved ILS (Percentage Increase in Life Span) with increasing doses (Doses of 50mg/kg, 100mg/kg and 200mg/kg showed ILS values of 13.70%, 19.20% and 21.92%, respectively) to some extent when compared with control group and groups receiving other extracts.Bangladesh J Microbiol, Volume 29, Number 2, Dec 2012, pp 70-74

scholarly journals Antioxidant, Antibacterial and Cytotoxic Activity of the Methanol Extract of Urtica Crenulata

2009 ◽  
Vol 2 (1) ◽  
pp. 169-177
Author(s):  
M. A. Rahman ◽  
M. S. Rana ◽  
M. M. Zaman ◽  
S. A. Uddin ◽  
R. Akter
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Lethality Assay ◽  
Stem Extract

The antioxidant, antibacterial and cytotoxic properties of methanol extract of Urtica crenulata (syn: Laportea crenulata Gaud) stem has been investigated in the present study. The antioxidant and cytotoxic activity of the Urtica crenulata methanol extract were assessed by DPPH free radical scavenging method and brine shrimp lethality bioassay method, respectively. The antibacterial activity of the plant extract (500 μg/disc) was also carried out by disc diffusion technique. Stem extract showed DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging effect compared with ascorbic acid. IC50 value of ascorbic acid and stem extract was found 14.72 μg/ml and 1468.9 μg/ml, respectively. In antibacterial experiment, Urtica crenulata stem extract showed 8, 14 and 10 mm of diameter of zone inhibition against Salmonella typhi, Shigella flexneri and Shigella sonnei, respectively and 9 and 8 mm of diameter of zone inhibition against Bacillus subtilis and Bacillus cereus but no activity was observed against Staphylococcus aureus. In brine shrimp lethality assay, the LC50 value of the extract was found 104.0 μg/ml, which indicates that the extract has high cytoxic effect. The present study demonstrates that methanol extract of Urtica crenulata stem has significant cytotoxic effect. The extract also showed some moderate antibacterial and minimum significant antioxidant effects.  Keywords: Urtica crenulata;  Antioxidant; Antibacterial; Cytotoxic; BHT. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i1.2872             J. Sci. Res. 2 (1), 169-177 (2010) 

scholarly journals In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR)

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Jahangir Khan Achakzai ◽  
Muhammad Anwar Panezai ◽  
Muhammad Ayub Kakar ◽  
Abdul Manan Kakar ◽  
Shahabuddin Kakar ◽  
...  
Keyword(s):  
Cell Line ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Aqueous Fraction ◽  
Brine Shrimp Lethality ◽  
Butanol Fraction ◽  
Whole Plant ◽  
Brine Shrimp Lethality Assay ◽  
Anti Inflammatory ◽  
Mcf 7

In this study, GC-MS analysis has shown that whole plant butanol fraction of rheum ribes (WBFRR) comprises of 21 compounds which exhibited anticancer (MCF-7) activity having IC50 value of 36.01± 0.26. MTT assay (MCF-7), Oxidative Burst assay using chemiluminescence technique, and B-Hatching techniques were the methods used for anticancer MCF-7, anti-inflammatory, and Brine Shrimp Lethality Assay (BSLA). GC-MS was used for structural elucidation. Whole plant methanol extract of rheum ribes (WMERR), whole plant n-hexane fraction of rheum ribes (WHFRR), and whole plant aqueous fraction of rheum ribes (WAFRR) were inactive against anticancer (MCF-7) cell line. Whole plant methanol extract of rheum ribes (WMERR), whole plant aqueous fraction of rheum ribes (WAFRR) and whole plant butanol fraction of rheum ribes (WBFRR) showed anti-inflammatory activity on ROS having IC50 value of 23.2±1.9, 24.2±2.7 and 12.0±0.6. Whole plant butanol fraction of rheum ribes (WBFRR) showed Brine Shrimp Lethality with LD50 693.302 while whole plant methanol extract of rheum ribes (WMERR) and whole plant aqueous fraction of rheum ribes (WAFRR) showed high lethality at highest concentration. This study revealed that whole plant butanol fraction of rheum ribes (WBFRR) exhibited significant anticancer (MCF-7) activity. In the near future, the constituent of whole plant butanol fraction of rheum ribes (WBFRR) can be the alternative drug against MCF-7 cell line with least toxicity and side effects.

scholarly journals 6. Cytotoxic Potential of n-Hexane Extract of Calotropis gigantea L. Leaves

2016 ◽  
Vol 1 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Kartini Hasballah ◽  
Murniana Murniana ◽  
Al Azhar ◽  
Nurul Rahmi
Keyword(s):  
Liquid Chromatography ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Cytotoxic Potential ◽  
Calotropis Gigantea ◽  
Brine Shrimp Lethality ◽  
Vacuum Liquid Chromatography

The present study was done to determine the cytotoxic potential of n-hexane extract of Calotropis gigantea L. leaves and its fractions. Here, dried leaves of C. gigantea L. were macerated using n-hexane to obtain crude extract of 21.16 g (1.03%). The components of n-hexane extract of C. gigantea L. leaves were separated with vacuum-liquid chromatography resulted 4 fractions which are A (0.5 g), B (0.9g), C (5.29 g), and D (6.25 g). Phytochemical screening indicated that the extract contained various secondary metabolic compounds such as steroids, terpenoids, saponins, flavonoids, coumarins, and phenolics. Cytotoxic potenital of the crude extract of C. gigantea L. and its fractions was examined using brine shrimp lethality test (BSLT). The LC50 values of C. gigantea’s n-hexane extract and its fractions that determined by Finney method were 272.27 (n-hexane extract), 31.62 (fraction A), 43.65 (fraction B), 33.89 (fraction C) and 20.98 (fraction D) ?g/mL, respectively. The most active was D fraction contained terpenoids, flavonoids, and phenolics which were assumed contribute to its cytotoxic potential. These results suggested that n-fraction of C. gigantea might possess antitumor or pesticidal activities..

scholarly journals Phytochemical Screening and Toxicological Evaluation Using Brine Shrimp Lethality Test (BSLT) of Some Fractions of Prasman Leaves (Eupatorium triplinerve V) Extract

2011 ◽  
Vol 2 (1) ◽  
pp. 193 ◽  
Author(s):  
Shirly Kumala ◽  
Dwi Windi Sapitri
Keyword(s):  
Toxicity Test ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Phytochemical Screening ◽  
Toxicological Evaluation ◽  
Brine Shrimp Lethality ◽  
Toxicological Effect ◽  
Toxicological Assessment ◽  
Study Results

Prasman leaves (Eupatororium triplinerve V) was well documented to have anti cancer benefit in Indonesian traditional medicine history. However, there were no scientific studies including toxicological assessment on the plan extract. The purpose of this study was to investigate the toxicological effect of some fractions of Prasman leaves methanol extract. Phytochemical screening by the Farnsworth method on powder and some fraction of the methanol extract were conducted followed by toxicity test using the “Brine Shrimp Lethality” test (BSLT) method. In the current study results, the phytochemical screening showed the presence of flavonoid, saponin, coumarin, tannin, steroid and volatile oil. LC50 of the n-hexane fraction 238.66 µg/mL, ethyl acetate fraction 24.42 µg/mL,  and n-butanol 64.10 µg/mL.Keywords : BSLT, Toxicity test, Eupatororium triplinerve

scholarly journals Phytochemicals, Antimicrobial and Cytotoxic Potential Study of Tinospora cordifolia

2020 ◽  
pp. 77-84
Author(s):  
Anik Barua ◽  
Md. Rabiul Hossain ◽  
Labonno Barua ◽  
Abu Sadat Md. Sayem ◽  
Md. Zainul Abedin ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Methanolic Extract ◽  
Antimicrobial Property ◽  
Biological Properties ◽  
Tinospora Cordifolia ◽  
Diffusion Method ◽  
Cytotoxic Potential ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Lethality Assay ◽  
Stem Extract

Aims: Tinospora cordifolia is a herb used in the traditional folk medicine due to their health benefits. Studies reported the biochemical composition and some biological properties of the plant stem. The aim of the current study was to investigate the antimicrobial and cytotoxic properties from the extract of Tinospora cordifolia stem. Place and Duration of Study: The study was accomplished by eleven months in the Department of Pharmacy, University of Science and Technology Chittagong (USTC), Bangladesh.  Methodology: Presence of phytochemical constituents such as alkaloids, flavonoids and tannins, saponins were evaluated. Antimicrobial activity of the stem extract was assayed separately using an agar diffusion method against some gram negative and gram positive bacteria. The cytotoxic potential of the stem extract was evaluated by the Brine-Shrimp Lethality Assay mathod.  Results: The zone of inhibition formed by the methanolic extract in the antimicrobial assays was significant. In addition, cytotoxicity of the extract was found to be significant as compared to the standard gallic acid in Brine-Shrimp lethality assay. Conclusion: The methanolic extract of Tinospora cordifolia may possess significant antimicrobial property which requires more studies to isolate the specific bioactive compound for developing a new antimicrobial agent from this plant.

scholarly journals Uji Toksisitas Akut Ekstrak dan Fraksi Kulit Batang Ketapang Laut (Terminalia Catappa L.) Menggunakan Metode BSLT

2020 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Ari Sartinah ◽  
Yamin Yamin ◽  
Muhammad Arba ◽  
Nur Illiyyin Akib ◽  
Andi Nafisah Tendri Adjeng ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Program Analysis ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Brine Shrimp Lethality ◽  
Terminalia Catappa ◽  
Water Fractions

AbstrakToksisitas akut merupakan kemampuan suatu bahan kimia (obat/bahan obat) dalam menimbulkan kerusakan  pada suatu organisme dalam waktu yang relatif singkat. Tujuan penelitian ini adalah untuk mengetahui potensi ketoksikan akut dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L.). Pengujian toksisitas akut dilakukan dengan menggunakan metode BSLT (Brine Shrimp Lethality Test) dan menggunakan analisis program untuk mengetahui nilai LC50 dari ekstrak dan fraksi. Hasil uji toksisitas akut menunjukan nilai LC50  dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L. )  berturut-turut 247,997 ppm; 400,666 ppm dan 618,046 ppm. Hal ini menunjukan bahwa ekstrak metanol batang ketapang laut (Terminalia catappa L.) bersifat paling toksik dibandingkan fraksi etil asetat dan fraksi air.Kata kunci: Ekstrak, Fraksi, Terminalia catappa L., BSLT, Artemia salina LeachAbstractAcute toxicity is the ability of chemical (drug/drug ingredient) to cause damage to organisms in a relatively short time. The purpose of this study was to determine the potential for acute toxicity of methanol extract, ethyl acetate and water fractions of sea ketapang stem skin (Terminalia catappa L.). Acute toxicity testing was performed using the BSLT (Brine Shrimp Lethality Test) method and program analysis to determine the LC50 values of extracts and fractions. The results of the acute toxicity test showed the LC50 values of methanol extract, ethyl acetate and water fractions of the plant stem skin consecutively were 247,997 ppm; 400,666 ppm and 618,046 ppm. This shows that the methanol extract of sea ketapang stems (Terminalia catappa L.) is the most toxic compared to the ethyl acetate and the water fraction.Keywords: Extract, Fraction, Terminalia catappa L., BSLT, Artemia salina Leach

scholarly journals Thrombolytic and Cytotoxic Activities of Terminalia bellerica Roxb.

2015 ◽  
Vol 16 (2) ◽  
pp. 131-135
Author(s):  
M Sekendar Ali ◽  
Kazi Omar Faruq ◽  
Atiqul Islam ◽  
AMM Nurullah ◽  
Kazi Ashfak Ahmed Chowdhury ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Thrombolytic Agent ◽  
Methanolic Extract ◽  
Pharmaceutical Journal ◽  
Cytotoxic Activities ◽  
Cytotoxic Potential ◽  
Thrombolytic Activity ◽  
Brine Shrimp Lethality ◽  
Terminalia Bellerica ◽  
Thrombolytic Effect

The present study was designed to investigate the thrombolytic activity and cytotoxic potential of the methanolic extract of bark of Terminalia bellerica Roxb. The cytotoxicity was assessed with the brine shrimp lethality bioassay and thrombolytic effect with human blood. The brine shrimp lethality bioassay was used to evaluate cytotoxicity (LC50 = 3.21 ?g/ml) compared to vincristine of sulphate (LC50 = 0.512 ?g /ml). It was also evaluated as thrombolytic agent as compared to streptokinase. It has significant thrombolytic activity (32.95%) compared to standard streptokinase (70%). DOI: http://dx.doi.org/10.3329/bpj.v16i2.22293 Bangladesh Pharmaceutical Journal 16(2): 131-135, 2013

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