Some Cytokines Levels (IL-6 and IL-10) in Sera Patients with Cutaneous Leismanasis in Nassiriya Province

2020 ◽  
pp. 4-6
Keyword(s):  
Serum Level ◽  
Immune Status ◽  
Pure Science ◽  
Control Subjects ◽  
Significant Difference ◽  
Healthy Control

The present study was carried out in the Labs of collage of education for pure science, during period from January 2017 to endDecember of the same year. The immune status investigates for CLpatients by measuring the levels of cytokines (IL6and IL10) in sera using a technique enzyme-linked immune Sorbent adsorptive (ELISA). The study included 120 subjects with (60 CLpatientsL.majar and 60 CLpatients L. tropica with and (30) were healthy control. Increased mean Serum level of IL6 was in the observed in the total patients as compared to control Subjects (224.53pg/ml,70.70pg/ml), the result indicate there was significant difference at (p<0.05) ,such observation was consistent in the patient infected with L.majar and L. tropica (104 .90 pg/ml and 112.78 pg/ml) respectively. The results of the IL10 showed significant difference at (p<0.05)increased of mean Serum level in the total CL patients as compared to control Subjects(226.90 pg/ml 46.77pg/ml,).Ahighly significant difference at (p<0.05) increased observed in patients group infected with L.majar and followed by patients group infected L. tropica (112.78pg/ml and 114.12pg/ml) respectively.These results revealed that the excessive presence of cytokines might play a role in CL patients.

scholarly journals Neutrophil-to-Lymphocyte and Platelet-to-Lymphocyte Ratios in Patients with RetinalArtery Occlusion

2020 ◽  
Author(s):  
Mahmut Atum ◽  
Gürsoy Alagöz
Keyword(s):  
Retinal Artery Occlusion ◽  
Artery Occlusion ◽  
Control Group ◽  
Retrospective Case ◽  
Control Subjects ◽  
Gender And Age ◽  
Significant Difference ◽  
Healthy Control ◽  
Retinal Artery ◽  
The Relationship

Purpose: This study aimed to compare the neutrophil-to-lymphocyte (NLR) and plateletto- lymphocyte (PLR) ratios in patients with retinal artery occlusion (RAO) with those from a healthy control population and to identify the relationship between them. Methods: Forty-six patients with RAO and fifty-one healthy control subjects were included in this retrospective case-control study. RAO was diagnosed following an ophthalmic examination and fluorescein angiography (FA). Blood neutrophil, lymphocyte, and platelet counts were recorded for each of the 97 subjects, from which NLR and PLR values were calculated. Results: There were 46 patients (28 male [M], 18 female [F]) in the RAO group and 51 patients (27 M, 24 F) in the control group. No significant differences were found between patients with RAO and the control subjects in terms of gender and age (P > 0.05). Patients with RAO had significantly increased NLR values (2.85 ± 1.70) than the control subjects (1.63 ± 0.59, P < 0.001). The mean PLR in patients with RAO was 123.69 ± 64.98, while that in control subjects was 103.08 ± 36.95; there was no significant difference between the two groups (P = 0.055). A logistic regression analysis revealed that NLRs were 3.8 times higher in patients with RAO than in control subjects (odds ratio = 3.880; 95% confidence interval = 1.94 to 7.74; P < 0.001). Conclusion: NLRs were significantly increased in patients with RAO compared to the control subjects.

scholarly journals General Immune Status and Oral Microbiology in Patients with Different Forms of Periodontitis and Healthy Control Subjects

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e109187 ◽  
Author(s):  
Jana Schmidt ◽  
Holger Jentsch ◽  
Catalina-Suzana Stingu ◽  
Ulrich Sack
Keyword(s):  
Immune Status ◽  
Control Subjects ◽  
Healthy Control ◽  
Oral Microbiology

MicroRNA-30e and MicroRNA-223 Expression for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C Virus

2020 ◽  
Vol 3 (1) ◽  
pp. 97-104
Author(s):  
Amany Ragab Youssef ◽  
Samah Bastawy ◽  
Karim Montasser ◽  
Sally Abed ◽  
Muhammad Diasty ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Early Diagnosis ◽  
Early Stage ◽  
Control Subjects ◽  
Combined Use ◽  
Significant Difference ◽  
Early Hcc ◽  
Healthy Control

Background. The goals of this study were to elucidate the use of the expression of microRNA-30e (miR-30e) and microRNA-223 (miR-223) as diagnostic biomarkers for early diagnosis of hepatocellular carcinoma (HCC) associated with hepatitis C virus (HCV) infection. Methods. The study included three groups, the first group included thirty patients with HCC associated with HCV, the second group included thirty patients with cirrhosis with HCV and the third group included thirty healthy control subjects. Blood samples were obtained for determination of serum expression of miR-30e and miR-223 by real time polymerase chain reaction. Results. There was significant decrease of miR-30e of expression in patients with HCC (0.16 ± 0.1) compared to both patients with cirrhosis (0.4 ± 0.2, P<0.01) and healthy control subjects (1.2 ± 0.4, P<0.001). There was also significant reduction of miR-223 expression levels in patients with HCC (0.2 ± 0.1) compared to patients with cirrhosis (0.5 ± 0.2, P<0.01) and healthy control subjects (1.0 ± 0.1, P<0.001). There was also significant decrease of miR-30e expression in late stage versus early stage of HCC (0.1 ± 0.0 vs. 0.22 ± 0.1, P<0.001), while there was no significant difference of alpha-fetoprotein (AFP) between patients with late and early HCC. Also, values of miR-223 had significantly reduced levels in late HCC compared to its expression values in early HCC (0.1± 0.0 vs. 0.23 ± 0.2, P<001). Conclusion. There was significant reduction of expression of miR-30e and miR-223 in serum of HCC patients compared to either patients with cirrhosis or healthy subjects. These results show that the combined use of both biomarkers had better sensitivity in the diagnosis of HCC compared to AFP.

Plasma Cysteine and Sulphate Levels in Patients with Cirrhosis of the Liver

1994 ◽  
Vol 87 (3) ◽  
pp. 357-362 ◽  
Author(s):  
M. H. Davies ◽  
L. Klovrza ◽  
R. H. Waring ◽  
E. Elias
Keyword(s):  
Liver Disease ◽  
Primary Biliary Cirrhosis ◽  
Intensive Therapy ◽  
Biliary Cirrhosis ◽  
Intensive Therapy Unit ◽  
Control Subjects ◽  
Significant Difference ◽  
Catabolic State ◽  
Healthy Control ◽  
Muscle Breakdown

1. Fasting levels of plasma cysteine, plasma sulphate and the plasma cysteine/sulphate ratio were measured in patients with primary biliary cirrhosis and compared with those in patients with other liver disease, general intensive therapy unit patients and healthy subjects. 2. Plasma cysteine was significantly elevated in patients with primary biliary cirrhosis (median 0.364 nmol/mg of protein, P < 0.0001) and patients with other liver disease (median 0.445 nmol/mg of protein, P < 0.0001), compared with healthy control subjects (median 0.125 nmol/mg of protein) and increased progressively with the severity of liver disease. Plasma cysteine was also elevated in intensive therapy unit patients (median 1.564 nmol/mg of protein) compared with healthy control subjects (P < 0.0001) and patients with other liver disease (P < 0.0001). 3. Plasma sulphate was reduced significantly only in patients with primary biliary cirrhosis (median 0.822 nmol/mg of protein) compared with healthy control subjects (median 1.37 nmol/mg of protein, P < 0.05). There was no significant difference in plasma sulphate between disease groups. 4. The plasma cysteine/sulphate ratio was significantly elevated in patients with primary biliary cirrhosis (median 0.448, P < 0.0001) and patients with other liver diseases (median 0.394, P < 0.0001) compared with healthy control subjects (median 0.095). The ratio was also elevated in intensive therapy unit patients (median 1.650, P < 0.0001) compared with healthy control subjects and liver disease groups (P < 0.0001). 5. In conclusion, plasma cysteine rises in primary biliary cirrhosis and other forms of liver disease. This effect is not specific to liver disease, since cysteine is elevated in an heterogeneous group receiving intensive care. Impairment of trans-methylation and trans-sulphuration pathways does not explain the finding of increased plasma cysteine. Since cysteine is elevated in non-hepatic disease, it may reflect the effect of muscle breakdown and the catabolic state. Impaired activity of cysteine dioxygenase and impaired mitochondrial function may be contributory, but this requires further study. These metabolic changes may reflect progressively diminished detoxification capacity within the liver and other tissues.

scholarly journals Evaluation of the optic nerve head vessel density in patients with limited scleroderma

2021 ◽  
Vol 13 ◽  
pp. 251584142199538
Author(s):  
Hilal Kılınç Hekimsoy ◽  
Ali Mehmet Şekeroğlu ◽  
Ali Mert Koçer ◽  
Vedat Hekimsoy ◽  
Ali Akdoğan
Keyword(s):  
Optical Coherence Tomography ◽  
Optic Nerve ◽  
Optic Nerve Head ◽  
Vessel Density ◽  
Optical Coherence ◽  
Control Subjects ◽  
Rnfl Thickness ◽  
Significant Difference ◽  
Healthy Control ◽  
Limited Scleroderma

Objectives: To investigate the optical coherence tomography angiography (OCTA) parameters of the optic nerve head and peripapillary retina and to assess macular and peripapillary retinal nerve fiber layer (RNFL) thickness by using spectral-domain optical coherence tomography (SD-OCT) in patients with limited scleroderma and to compare these results with those of healthy control subjects. Materials and Methods: 42 patients with a confirmed diagnosis of limited scleroderma and 32 age- and sex-matched healthy control subjects were included in the study. OCTA was performed for the radial peripapillary capillary plexus (RPCP) whole image, inside disc, and peripapillary vessel densities in all participants with XR Avanti AngioVue OCTA (Optovue, Fremont, California, USA). OCT images were obtained with Spectralis OCT with eye-tracking dual-beam technology (Heidelberg Engineering GmbH, Heidelberg, Germany), and peripapillary RNFL thickness was evaluated with circle program. The data from the right eyes of all participants were used for statistical analysis. Results: No significant difference was found between the radial RPCP whole image, inside disc, and peripapillary vessel density values or the RNFL parameters of the scleroderma patients when compared with the controls ( p > 0.05 for all). Conclusion: Decreased peripapillary vessel density on OCTA, which can be an early sign of glaucoma, could not be observed in scleroderma patients in this study. However, further long-term studies are still needed to identify glaucoma tendency in patients with scleroderma before clinically detectable glaucoma.

Impairment of the antioxidant properties of serum albumin in patients with diabetes: protective effects of metformin

2008 ◽  
Vol 114 (3) ◽  
pp. 251-256 ◽  
Author(s):  
Patrice Faure ◽  
Nicolas Wiernsperger ◽  
Camille Polge ◽  
Alain Favier ◽  
Serge Halimi
Keyword(s):  
Oxidative Stress ◽  
Fluorescence Quenching ◽  
Serum Albumin ◽  
Structural Changes ◽  
Antioxidant Properties ◽  
Protective Effects ◽  
Control Subjects ◽  
Significant Difference ◽  
Patients With Diabetes ◽  
Healthy Control

Free radical production is increased during diabetes. Serum albumin is a major antioxidant agent, and structural modification of albumin induced by glucose or free radicals impairs its antioxidant properties. Therefore the aim of the present study was to compare the antioxidant capacities and structural changes in albumin in patients with T2DM (Type 2 diabetes mellitus) treated with MET (metformin) or SU (sulfonylureas) and in healthy control subjects. Structural changes in albumin were studied by fluorescence quenching in the presence of acrylamide. Albumin thiols and fructosamines, reflecting oxidized and glycation-induced changes in serum albumin respectively, were assessed. Structural changes in albumin were demonstrated by a significant decrease in fluorescence quenching in patients with T2DM, with patients treated with MET exhibiting a significant difference in the conformation of albumin compared with patients treated with SU. Oxidation, resulting in a significant decrease in thiol groups and plasma total antioxidant capacity, and glycation, associated with a significant increase in fructosamines, were both found when comparing healthy control subjects with patients with T2DM. When patients treated with MET were compared with those treated with SU, oxidative stress and glycation were found to be significantly lower in MET-treated patients. In conclusion, patients with T2DM have a decrease in the antioxidant properties of serum albumin which may aggravate oxidative stress and, thus, contribute to vascular and metabolic morbidities. Moreover, a significant protection of albumin was found in patients with T2DM treated with MET.

Gastric Carcinoma and Thyroid Status

2005 ◽  
Vol 33 (2) ◽  
pp. 222-227 ◽  
Author(s):  
EG Kandemir ◽  
A Yonem ◽  
Y Narin
Keyword(s):  
Gastric Cancer ◽  
Gastric Carcinoma ◽  
Autoimmune Thyroid Disease ◽  
Serum Levels ◽  
Thyroid Disorders ◽  
Thyroid Status ◽  
Autoimmune Thyroid ◽  
Control Subjects ◽  
Significant Difference ◽  
Healthy Control

Gastric carcinoma is reported to be more frequent in geographical areas where diets are either iodine-deficient or iodine-excessive. Reports have also shown an association between thyroid diseases and some of the risk factors for gastric carcinoma. We investigated the frequency of thyroid disorders in 61 patients with gastric carcinoma compared with 55 healthy control subjects. Thyroid health was evaluated by physical examination and by measuring the serum levels of thyroid hormones and thyroid autoantibodies. More patients with gastric cancer had goitre compared with healthy controls (49.1% versus 20%, respectively). Significantly more patients with gastric cancer had non-toxic goitre compared with control subjects. There was also a significant difference in the incidence of autoimmune thyroid disease −27.8% of patients with gastric cancer versus 10.9% of control subjects were affected. These results indicate that there is a significant association between gastric cancer and thyroid disorders.

scholarly journals Morphometric and hemodynamic analysis of atherosclerotic progression in human carotid artery bifurcations

2016 ◽  
Vol 310 (5) ◽  
pp. H639-H647 ◽  
Author(s):  
Xu Huang ◽  
Xiaoping Yin ◽  
Yingjin Xu ◽  
Xinwei Jia ◽  
Jianhui Li ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Carotid Artery Disease ◽  
Three Dimensional ◽  
External Carotid Artery ◽  
External Carotid ◽  
Control Subjects ◽  
Carotid Artery Bifurcation ◽  
Significant Difference ◽  
Healthy Control ◽  
Artery Disease

Although atherosclerosis has been widely investigated at carotid artery bifurcation, there is a lack of morphometric and hemodynamic data at different stages of the disease. The purpose of this study was to determine the lesion difference in patients with carotid artery disease compared with healthy control subjects. The three-dimensional (3D) geometry of carotid artery bifurcation was reconstructed from computed tomography angiography (CTA) images of Chinese control subjects ( n = 30) and patients with carotid artery disease ( n = 30). We defined two novel vector angles (i.e., angles 1 and 2) that were tangential to the reconstructed contour of the 3D vessel. The best-fit diameter was computed along the internal carotid artery (ICA) center line. Hemodynamic analysis was performed at various bifurcations. Patients with stenotic vessels have larger angles 1 and 2 (151 ± 11° and 42 ± 20°) and smaller diameters of the external carotid artery (ECA) (4.6 ± 0.85 mm) compared with control subjects (144 ± 13° and 36 ± 16°, 5.2 ± 0.57 mm) although there is no significant difference in the common carotid artery (CCA) (7.1 ± 1.2 vs. 7.5 ± 1.0 mm, P = 0.18). In particular, all patients with carotid artery disease have a stenosis at the proximal ICA (including both sinus and carina regions), while 20% of patients have stenosis at the middle ICA and 20% have stenosis expansion to the entire cervical ICA. Morphometric and hemodynamic analyses suggest that atherosclerotic plaques initiate at both sinus and carina regions of ICA and progress downstream.

Comparison of in vitro glioma cell cytotoxicity of LAK cells from glioma patients and healthy subjects

1988 ◽  
Vol 69 (2) ◽  
pp. 234-238 ◽  
Author(s):  
Vidar Bosnes ◽  
Henry Hirschberg
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Lak Cells ◽  
Control Group ◽  
Peripheral Blood Mononuclear ◽  
Control Subjects ◽  
Significant Difference ◽  
Blood Mononuclear Cells ◽  
Healthy Control

✓ Peripheral blood mononuclear cells from 11 glioma patients and 11 healthy control subjects were cultured in medium containing recombinant interleukin-2 for a period of 5 days. The cytotoxicity of these lymphokine-activated killer (LAK) cells was tested on chromium-51-labeled freshly prepared allogeneic glioblastoma cells, and on the cell lines K562 (natural killer cell (NK)-sensitive) and Daudi (NK-resistant). Peripheral blood mononuclear cells from all subjects showed high levels of cytotoxicity against these targets. There was no significant difference between the patients and the control group when LAK cytotoxicity was compared. Thus, although glioma patients are known to have depressed immunological reactivity, the cytotoxic capacity of LAK cells derived from glioma patients is similar to that of LAK cells from healthy control subjects. However, the glioma patients had significantly reduced numbers of mononuclear cells in their peripheral blood, possibly due to steroid treatment. Therefore, the volume of blood required to generate the same number of LAK cells was approximately three times larger from the glioma patients than from control subjects.

Urinary Glycosaminoglycan Excretion in Rheumatic Diseases

1992 ◽  
Vol 38 (6) ◽  
pp. 841-846 ◽  
Author(s):  
V Kéry ◽  
M Orlovská ◽  
M Stancíková ◽  
M Risko ◽  
D Zlnay
Keyword(s):  
Rheumatoid Arthritis ◽  
Ankylosing Spondylitis ◽  
Decision Analysis ◽  
Alcian Blue ◽  
Rheumatic Diseases ◽  
Clinical Decision ◽  
Control Subjects ◽  
Significant Difference ◽  
Healthy Control ◽  
Urine Specimens

Abstract We used Alcian Blue (AB) and dimethylmethylene blue (DMB) methods to measure glycosaminoglycan (GAG) excretion in the first morning urine specimens of patients with osteoarthritis (OA), ankylosing spondylitis (AS), and rheumatoid arthritis (RA) in different stages of disease. By the AB method, urinary GAG excretion in patients with RA was not different from healthy control subjects. However, the DMB method showed significant differences (in milligrams of GAG per gram of creatinine) for OA (median 25.4, range 14.3-44.0, P less than 0.01, n = 23) and RA patients (median 33.0; range 10.0-147.6; P less than 0.001, n = 63) in comparison with unaffected individuals (median 20.2; range 8.9-41.4, n = 38). We noted a significant difference in urinary GAG excretion between RA and OA patients (P less than 0.01) and between RA and AS (P less than 0.01) patients. The DMB method was further investigated by clinical decision analysis. The DMB method is simple and rapid and may be useful in diagnosing RA by distinguishing between RA and OA or AS.

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