The Influence of Leaf Fertilizer Media on Multiplication of Barangan Banana (Musa acuminate L.) in vitro

Barangan banana (Musa acuminata L.) is a plant that has the biggest contribution to national fruit production. The increasing demand for the banana requires the availability of seedlings, while conventional cultivation is unable to produce healthy, disease-free seedlings in a short time and large amount. In vitro culture is considered to be able to handle the constraints of the conventional seed supply. The purpose of this study was to determine the effect of foliar fertilizers on in vitro multiplication of barangan bananas. The method used was a single factor of Completely Randomized Design (CRD), the addition of leaf fertilizer consisting of five levels of media treatment, media MS (control), and 0.50 ml L-1 (p1); 0.75 ml L-1 (p2); 1.00 ml L-1 (p3); 1.25 ml L-1 (p4); 1.50 ml L-1 (p5) of leaf fertilizer media repeated five times. The data were analyzed with Analysis Variance then followed by Duncan Multiple Ranged Test of 5%. The results showed MS media still gave the best results compared to other leaf fertilizer media. Meanwhile, the leaf fertilizer media used in this study still gave good results on the initial time of buds appearance (p3 treatment), the number of shoots and the number of leaves in the p5 treatment, as well as the height of the plant in the p4 and p5 treatments. Therefore, the leaf fertilizer can be used to substitute MS media

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Evaluation of different growth hormones mediated callus induction and regeneration as well as the effect of colchicines, ethyl methanesulfonate (EMS) and gamma radiation on some traits of Impatiens walleriana

Genetika ◽

10.2298/gensr2101379s ◽

2021 ◽

Vol 53 (1) ◽

pp. 379-391

Author(s):

Sedigheh Sharafi ◽

Pejman Azadi ◽

Maryam Jafarkhani Kermani ◽

Vahid Abdossi ◽

Ali Eskandri

Keyword(s):

Dry Weight ◽

Control Treatment ◽

Lower Survival Rate ◽

Randomized Design ◽

Effective System ◽

Number Of Leaves ◽

Impatiens Walleriana ◽

The Impact ◽

Disease Free

Impatiens is an ornamental member of family Balsaminaceae. This plant mostly propagated by vegetative technique, which generally time wasting process. It is often multiplicities via seed but is barricaded by F1 seed sterility. In vitro culture of Impatiens walleriana has much significant function in fast proliferation with useful features and elicitation of healthful and disease-free plants. This experiment was conducted to investigate the impact of medium and different hormones on in vitro propagation of Impatiens walleriana by using a completely randomized design. MS medium was prepared along with various concentrations of BAP, TDZ and ZEA. Callus was induced and grew well in media supplemented with 0.5 mg/l NAA + 1 mg/l BAP. In order to indirect propagation, explants were cultured in same media containing BAP, ZEA and TDZ in combination with NAA. These treatments have ability to organogenesis. The results revealed that the control treatment had the lowest effect on traits including shoot percentage, number of shoots, number of leaves, shoot length, fresh and dry weight, and it lead to maximum proliferations in medium supplemented with 0.5 mg/l NAA + 1 mg/l BAP. The highest root length and rooting percentage was observed in 0.5 mg/l IBA + 0.5 mg/l BAP. In addition, the effect of mutation agents was studied. Aseptic samples were treated with ?- irradiation, Ethyl Methane Sulfonate and colchicines at growth chamber. Treatments with 30 and 60 grey respectively had the lower survival rate, growth rate and polyploidy while colchicines with 0.1 and 0.2 had the highest rats. Regarding to these, the present technique illustrate an effective system for in vitro reproduction of Impatiens walleriana by hypocotyls cultures. In addition, colchicines proved to be effective in induction of polyploidy in this plantlet.

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The Effect of Benzyl Amino Purine (BAP) Concentration on the Growth Amount of the Explant of Dendrobium spectabile Orchid by In-Vitro

International Journal of Multi Discipline Science (IJ-MDS) ◽

10.26737/ij-mds.v3i2.2397 ◽

2020 ◽

Vol 3 (2) ◽

pp. 33

Author(s):

Ario Ario ◽

Setiawan Setiawan

Keyword(s):

Economic Value ◽

Aloe Vera ◽

Leaf Length ◽

Plant Seeds ◽

Randomized Design ◽

In Vitro Technique ◽

Number Of Leaves ◽

Short Time ◽

Benzyl Amino Purine

Dendrobium spectabile orchid is an ornamental plant of high economic value, having relatively durable properties, beauty, and attractiveness of orchids lie in the diverse shapes and colors of the flowers. Therefore, it requires rapid and efficient propagation techniques. One of them is in-vitro vegetative propagation. Plant propagation using the in-vitro technique has the opportunity to produce a large number of plant seeds in a relatively short time. This study aims to determine the correct concentration of Benzyl Amino Purine (BAP) on the growth amount of the explants of Dendrobium spectabile orchid by in-vitro. The research was conducted at the Biotechnology Laboratory of the Regional Technical Implementation Unit (UPTD) of the Aloe Vera Center (AVC) Pontianak. This research was conducted from January 1, 2020, to March 30, 2020. The method used was a Completely Randomized Design (CRD). The treatment consisted of 6 levels of treatment, namely b1 = 0.75 ppm, b2 = 1.00 ppm, b3 = 1.25 ppm, b4 = 1.50 ppm, b5 = 1.75 ppm, b6 = 2.00 ppm. All treatments were repeated 4 times for each treatment consisting of 3 plant samples. The research finding showed that the treatment of BAP concentration 1.50 was the best in increasing the average number of leaves 4.92 strands, the average number of shoots 4.25 buds, and the average leaf length was 2.44 cm.

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Substitusi Media Murashige dan Skoog/MS dengan Air Kelapa dan Pupuk Daun Majemuk pada Pertumbuhan Anggrek Dendrobium secara in vitro (In Vitro Growth of Dendrobium Orchids under Substitution Murashige dan Skoog/MS Medium With Coconut Water and Compound Leaf Fertilizer)

JURNAL BIOS LOGOS ◽

10.35799/jbl.6.1.2016.16258 ◽

2016 ◽

Vol 6 (1) ◽

Author(s):

Annatje E.B. Inkiriwang ◽

Jeany Mandang ◽

Semuel Runtunuwu

Keyword(s):

Plant Height ◽

Coconut Water ◽

Ms Medium ◽

In Vitro Growth ◽

Compound Leaf ◽

Randomized Design ◽

Shoot Number ◽

Number Of Leaves ◽

Leaf Fertilizer

Abstrak Penelitian ini bertujuan untuk menentukan konsentrasi substitusi air kelapa dan pupuk daun majemuk yang tepat pada media MS terhadap pertumbuhan anggrek Dendrobium secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan dan 10 kali ulangan. Variabel yang diamati adalah persentase eksplan yang bertunas, jumlah tunas, jumlah daun, tinggi tanaman dan jumlah akar. Hasil penelitian menunjukkan substitusi media MS 50 %, air kelapa 30 % dan pupuk daun majemuk (Growmore®) 1,5 g/L menghasilkan rata-rata persentase eksplan yang bertunas sebesar 6,78 %; jumlah tunas sebesar 1,26 dan tinggi tanaman sebesar 1,20 cm.Kata kunci: air kelapa, in vitro, media MS, pupuk daun majemuk Abstract This research was aimed to determine the proper concentration of substitution of coconut water and compound leaf fertilizer in Murashige dan Skoog (MS) medium on the in vitro growth of Dendrobium orchids. The completely randomized design experiment consisted of 7 treatments and 10 replicates. The measured parameters were the percentage of sprouted explants, number of shoots, number of leaves, plant height and number of roots. The result showed that the substitution of 50% media MS, 30% coconut water and 1.5 g/L leaf fertilizer (Growmore®) resulted the growth of shoot explant as much as 6.78% the shoot number of 1.26 and 1.20 cm plant height .Keywords: coconut water, compound leaf fertilizer, in vitro, MS medium

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Multiplikasi Tunas Meriklon Kentang Pada Berbagai Jenis dan Konsentrasi Sitokinin

Agrologia ◽

10.30598/a.v7i2.766 ◽

2018 ◽

Vol 7 (2) ◽

Author(s):

Mia Munggarani ◽

Erni Suminar ◽

Anne Nuraini ◽

Syariful Mubarok

Keyword(s):

Shoot Multiplication ◽

Variable Number ◽

Seed Technology ◽

Randomized Design ◽

Meristem Explants ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Short Time ◽

Disease Free ◽

Number Of Branches

Mericlone shoots are shoots of propagation using meristem explants which aim to produce seeds in a short time, large number, and disease-free seed. The aim of this experiment was to find out the best types and concentration of cytokinin to mericlone shoot multiplication of potato Jala Ipam variety. The experiment was carried out at Seed Technology Tissue Culture Laboratory of Agriculture, Padjadjaran University during November 2016 until February 2017. The experimental design was Completely Randomized Design with 13 treatments and 4 replications. Murashige and Skoog Medium with different concentration and types of cytokinine was used in this experiment. The result showed that the type and concentrations of cytokinin give the different effect on the mericlone shoot multiplication of potato Jala Ipam variety. MS medium with the additional of cytokinin 2 -iP 1.5 mg L-1 significantly increased the mericlone shoot multiplication of potato Jala Ipam variety at the variable number of leaves, number of nodes, and the number of branches.

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Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

Jurnal SAINS ALAMI (Known Nature) ◽

10.33474/j.sa.v3i2.7222 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Wulan Dari Neng Gumiwang ◽

Tintrim Rahayu ◽

Ari Hayati

Keyword(s):

Root Length ◽

Cocos Nucifera ◽

Water Concentration ◽

Coconut Water ◽

Culture Bottle ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%) dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan

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PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

AGRI-SOSIOEKONOMI ◽

10.35791/agrsosek.14.1.2018.19730 ◽

2018 ◽

Vol 14 (1) ◽

pp. 439

Author(s):

Alfrida ., Maninggolang ◽

Jeany Sh. Polii-Mandang ◽

Wenny ., Tilaar

Keyword(s):

Brassica Oleracea ◽

Coconut Water ◽

Shoot Bud ◽

Randomized Design ◽

Wet Weight ◽

Number Of Leaves ◽

Brassica Oleracea L ◽

Benzyl Amino Purine ◽

Number Of Shoots

This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.

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Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.6.1.20-28 ◽

2015 ◽

Vol 6 (1) ◽

pp. 20

Author(s):

Erna Sinaga ◽

Megayani Sri Rahayu ◽

Awang Maharijaya

Keyword(s):

Tissue Culture ◽

Drought Tolerance ◽

In Vitro Selection ◽

Solanum Melongena ◽

Survival Percentage ◽

Drought Tolerant ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design

ABSTRACTThe objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought tolerance of eggplant and the drought tolerant eggplant accessions. The experiment was conducted at the Laboratory of Tissue Culture, Department of Agronomy and Horticulture, Bogor Agricultural University. The experiment was arranged in a completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10, and 15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090). The results showed that the addition of PEG to in vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought ABSTRAKPenelitian ini bertujuan untuk mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG) terhadap pertumbuhan tanaman terung in vitro, mendapatkan konsentrasi PEG yang dapat digunakan untuk seleksi tanaman terung secara in vitro dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan, Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor. Penelitian ini disusun dalam rancangan acak lengkap dua faktor. Faktor pertama adalah konsentrasi PEG terdiri atas 0, 5, 10, dan 15%. Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090). Hasil penelitian menunjukkan bahwa penambahan PEG pada media in vitro memberikan pengaruh nyata dan sangat nyata terhadap persentase hidup eksplan, persentase eksplan berkalus, pertambahan tinggi tunas, dan jumlah daun tanaman terung. Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan

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EFFECT OF CYTOKININS ON IN VITRO SHOOT PROLIFERATION OF OLIVE CULTIVARS “EARLIK” AND “BARI ZAITOON-2”

World Journal of Biology and Biotechnology ◽

10.33865/wjb.003.02.0156 ◽

2018 ◽

Vol 3 (2) ◽

pp. 199

Author(s):

Sharmin Ashraf ◽

Ayesha Manzoor ◽

Bushra Zulfiqar ◽

Muhammad A. Tariq

Keyword(s):

Woody Plants ◽

Shoot Proliferation ◽

Treatment Results ◽

Effective Technique ◽

Randomized Design ◽

Olive Cultivars ◽

Micro Propagation ◽

Completely Randomized Design ◽

Disease Free

In olive, micro-propagation is an effective technique for mass multiplication of disease free, true to type plants, but shoot proliferation in mature tissues of olive is a major difficulty encountered during culture establishment. Thus an experiment was designed with an objective to study the effect of different cytokinins (BAP and Zeatin) on shoot proliferation of two olive cultivars (Earlik and BARI Zaitoon-2). In olive Rugini medium, BAP and Zeatin were added alone and in combination at a concentration of 1 mg L-1 and 2 mg L-1. Experiments were arranged according to completely randomized design (CRD) with three replications per treatment. Results showed that both the olive cultivars performed well when BAP + Zeatin were supplemented to the medium at 2+2 mg L-1 concentration. However, the cultivar “BARI Zaitoon-2” had the highest shooting percentage (90.67 %) and number of nodes per shoot (8.33) in treatment containing BAP (2 mg L-1) + Zeatin (2 mg L-1), whereas, “Earlik” at same concentration had more shoot length (9.10 cm) and number of shoots per explant (3.67). Thus, it was concluded that the findings of these results will help in the future for developing shoot proliferation protocols for other olive cultivars. Moreover, these protocols can also be further be used in other woody plants micro propagation

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ADDITION OF THIAMIN AND LEAF FOLIAR IN ABACA BANANA (Musa textillis Nee.) ACCLIMATIZATION

Agrivet ◽

10.31315/agrivet.v25i2.4286 ◽

2021 ◽

Vol 25 (2) ◽

pp. 88

Author(s):

Rina Srilestari ◽

Suwardi Suwardi

Keyword(s):

Raw Materials ◽

Paper Industry ◽

Economic Value ◽

Large Space ◽

Planting Material ◽

Randomized Design ◽

Number Of Leaves ◽

The Right ◽

Leaf Fertilizer ◽

Range Test

The economic value of the Abaca banana is on the trunk which contains fiber for valuable textile and paper industry raw materials. The multipurpose fiber and its prospects are quite good, so the abaca plant gets a lot of attention from various community groups both private, state-owned, cooperative and farmers. Propagation through multiplication humps is very low, planting material is easily damaged in transportation, is not durable and requires large space so that transportation costs are high. One alternative to overcome the provision of healthy, large and fast seedlings is to use seedlings from tissue culture. The aim of the study is to get the right thiamin and leaf fertilizer concentrations at the acclimatization stage. The experimental design used was a 2-factor Complete Randomized Design, Thiamin concentration (1 ml / l, 2 ml / l, 3 ml / l) and leaf fertilizer (0.5 ml / l, 1 ml / l, 1.5 ml / l). The data obtained were analyzed for diversity at 5% level and further tests using Duncan Multiple Range Test (DMRT) level of 5%. The results showed no interaction between thiamin and leaf fertilizer, Thiamin 2 ml / l gave the best results on all parameters and 1.5 ml / l leaf fertilizer could increase plant height and the number of leaves of abaca banana plants.Keywords: Thiamin, leaf fertilizer, abaca banana, acclimatization

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In vitro development of Encyclea cordigera in different concentrations of honey

Ciência Rural ◽

10.1590/0103-8478cr20150046 ◽

2015 ◽

Vol 46 (4) ◽

pp. 590-592 ◽

Cited By ~ 2

Author(s):

Cibele Mantovani ◽

Kathia Fernandes Lopes Pivetta

Keyword(s):

Culture Media ◽

Leaf Length ◽

In Vitro Development ◽

Randomized Design ◽

Sucrose Medium ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Set Up ◽

Vitro Development

ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige & Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf, length of the longest root, number of leaves, and fresh and dry masses were evaluated. Data concerning the number of leaves and roots were (x+1)1/2 transformed and subjected to an analysis of variance (ANOVA); the means were compared by a Tukey's test set at 5% probability. Medium containing 60g L-1 of honey proved to be superior to the sucrose medium traditionally used, favoring the in vitro growth and development of Encyclea cordigera. This medium can therefore be recommended for the propagation of this species, which is usually cultivated as an ornamental plant.

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