scholarly journals Assessment of the occurrence of gene polymorphisms CAPN316 and UoGCAST in the population of cattle

2019 ◽  
Vol 10 (1) ◽  
pp. 4786-4789
Keyword(s):  
Gene Polymorphism ◽  
Dna Markers ◽  
Gene Polymorphisms ◽  
Molecular Genetic ◽  
Farm Animals ◽  
Heterozygous State ◽  
Endogenous Inhibitor ◽  
Study Population ◽  
Calpain System ◽  
Homozygous Form

To solve the problem of increasing the rate of breeding, but reduce the breeding work with farm animals, it is necessary to form herds with the desired level of productivity, that are adapted to specific regions of breeding, resistant to various diseases, with a decrease in the time for the breeding process. The active use of molecular genetic methods has contributed to the expansion of the list of DNA markers for farm animals, which are candidate genes for economically useful traits. Among these genes are widely known members of the calpain-calpastatin system, which is associated with postmortem proteolysis and tenderisation of muscles. The calpain system consists of an actively expressed μ-calpain (CAPN1) and m-calpain (CAPN2) and a single endogenous inhibitor, CAPN1 and CAPN2-calpastatin (CAST). The study of polymorphisms of these genes contributes to the expansion of marker characterisation in breeding. DNA samples (n=139) obtained from the blood of cattle were used in the work. Real-time PCRs were carried out using a ANK-32 programmable thermocycler (Synthol, Moscow, Russia). The CAPN316 gene polymorphism was present in 15% of animals tested, with allele G being found in 85% of animals. Similar calculations on the occurrence of the UoGCAST gene polymorphism in this sample of animals found the desirable allele G in 22% of animals and allele C in the remaining 78%. From the analysis of the occurrence of both genes and their polymorphisms in the study population, animals that had a combination of both desirable genotypes (CC*GG*) of CAPN316 and UoGCAST genes were not identified. There were also no animals that had the desired CAPN316 CC genotype and the heterozygous state of the UoGCAST gene (CC*GC). While 7.6% of the animals were CC*GG for the desirable expression of gene CAPN316, they contained the homozygous form of the gene UoGCAST.

scholarly journals CHARACTERISTICS OF UKRAINIAN GREY CATTLE BY DNA-MARKERS

2018 ◽  
Vol 51 ◽  
pp. 283-289 ◽  
Author(s):  
N. Mokhnachova ◽  
T. Suprovich ◽  
M. Dobrynska ◽  
N. Fursa
Keyword(s):  
Growth Hormone ◽  
Gene Polymorphism ◽  
Milk Production ◽  
Dna Markers ◽  
Farm Animals ◽  
Homozygous Genotype ◽  
Thyroglobulin Gene ◽  
Meat Productivity ◽  
Beta Lactoglobulin ◽  
Calpain Gene

Today conservation of biodiversity is one of the most important priorities in the world. Polymorphism of farm animals is a key to successful breeding; it provides animal adaptation to environmental changes. Impoverishment of genetic resources of farm animals can lead to various negative consequences, such as significant decrease of the effectiveness of selection, existing breeds will not be able to successfully resist to infectious agents evolving constantly; valuable material for the study and analysis of breed origin will be lost. Local breeds created by national selection are valuable genetic resources. Lacking high performance in most cases, they are usually characterized by high resistance to various diseases. Ukrainian Grey cattle, as a representative of the local native breeds, are an interesting object of population research in respect not only of adaptive characteristics, but also genetic mechanisms providing phenotypic expression of certain features of productivity. Recent advances in molecular genetics have made it possible to identify genes associated with qualitative and quantitative characteristics of cattle. The most informative one in this regard is the DNA marker systems based on structural analysis of polymorphisms of genes involved in formation and operation of economically useful traits. The most common potential DNA markers of characteristics of cattle productivity include genes of growth hormone (bGH), beta-lactoglobulin (βLG), thyroglobulin (TG5), calpain (CAPN). BGH gene is an important regulator of somatic growth of animals and has lactating and fat-mobilizing effects. CSN3 gene is linked to milk protein ability and technological properties of milk. Different allelic variants of βLG gene are associated with high content casein and whey proteins in milk, fat percentage and positive impact on milk production. Milk products and falsification detection of milk are controlled by this gene. Its role has been proved in antimicrobial activity to pathogens of mastitis. TG5 gene is a precursor of tyreoid hormones such as triiodothyronine and tetraiodothyronine participating in formation of fat cells and the formation of meat marbling. CAPN gene is involved in proteolysis during ripening of meat and leads to higher tenderness of meat. The aim was to investigate the allelic polymorphism of genes of growth hormone, beta-lactoglobulin, thyroglobulin and calpain in Ukrainian Grey cattle. Blood samples (n = 136) from Ukrainian Grey cattle at "Markeyevo" breeding farm (Kherson region) were studied. Molecular genetic studies were conducted at Laboratory of Genetics of Institute of Animal Breeding and Genetics nd. a. M.V. Zubets of NAAS. DNA isolation from whole blood was performed using standard commercial kit "DNA-Sorb-B" (produced by AmpliSens, Central Research Institute of Epidemiology, Russia). Polymorphisms of genes of GH, βLG5, TG and CAPN1530 was investigated by PCR-RFLP. Electrophoretic separation of restriction fragments of DNA was performed in 1.5% and 2% agarose gels in tris-borate electrophoresis buffer. The results of DNA testing of beta-lactoglobulin locus for A- and B-allele variants in animals of Ukrainian Grey breed found that most of the cows were carriers of homozygous BB genotype of βLG gene. It was determined at every second investigated animals. Homozygous AA genotype was found only at two cows or it is 4%. AB genotype was represented at 45% of animals. Thus, the study found a significant prevalence of frequencies B allele over A allele (0.736 and 0.264, respectively), which leads to overwhelming homozygotisation of one of these alleles. B allele in beta-lactoglobulin gene is associated with high content of casein protein in milk and a high percentage of fat. The values of expected and observed heterozygosity at this gene were not significantly different. The study of gene polymorphism of thyroglobulin found that at the population of the represented breed, the most often seen heterozygous genotype was CT, carriers of which were 57% of the animals. In homozygous state C allele was in 31% of the animals, and T allele – only in 11%. Regarding the level of heterozygosity, we received that actual heterozygosity exceeded theoretically expected one. However, difference is statistically insignificant. Consequently, for thyroglobulin gene Ukrainian Grey cattle belong to breeds that carry the highest frequency of desired C allele for marbling of meat. The analysis of gene polymorphism of bGH indicates the absence of the animals with genotype VV in the investigated sampling, low percentage of heterozygotes (3%) and a significant portion of homozygotes for L allele (98%). Significant differences on levels of actual and expected heterozygosity for somatotropin gene we haven’t found. One of the markers of quality characteristics of meat productivity of cattle is CAPN gene. In general, the animals of Ukrainian Grey breed are characterized by the absence of polymorphism for calpain gene. All the studied animals were carriers of homozygous genotype for preferred G allele (1,0). This feature of the genetic structure of the studied population by calpain gene, animals of which are reproduced in a small array, shows breed-specific high genetic potential by quality characteristic of meat productivity, namely, the tenderness of meat. It was revealed that the population of Ukrainian Grey breed was characterized by low level of polymorphism for genes which cause milk production and meat quality indicators. Thus, homozygous genotype BB dominated for beta-lactoglobulin gene; homozygous LL genotype (98%) was also significantly prevalent and VV genotype was not detected for growth hormone gene; only genotype GG was generally found for calpain gene. And heterozygous CT genotype (57%) dominated only for TG5 locus of thyroglobulin gene.

scholarly journals Effect of IL4-589C>T, FCGR2A-166His>Arg, DEFB1-20G>A, DEFB1-52G>A gene polymorphisms on TNFα, IL-1β, IL-4, and IL-10 contents in the patients with primary hip osteoarthrosis

2020 ◽  
Vol 22 (5) ◽  
pp. 1009-1016
Author(s):  
A. M. Miromanov ◽  
T. V. Zabello ◽  
N. A. Miromanova
Keyword(s):  
Gene Polymorphism ◽  
Gene Polymorphisms ◽  
Inflammatory Diseases ◽  
Molecular Genetic ◽  
Vascular Diseases ◽  
Fold Increase ◽  
Molecular Genetic Analysis ◽  
Radiographic Examination ◽  
Control Group ◽  
Hip Joints

Our objective was to study the effects of IL4-589C>T, FCGR2A-166His>Arg, DEFB1-20G>A, DEFB1-52G>A gene polymorphisms upon content of TNFα, IL-1β, IL-4, and IL-10 in primary osteoarthrosis of the hip joints. We performed a survey of 100 patients of Russian ethnicity (average age 61.3±8.5 years) with primary coxarthrosis at the stage III-IV who lived in the Trans-Baikal region. The control group (n = 100), were local residents, comparable by age (60±8.3 years), gender, habitation place and nationality. The exclusion criteria were as follows: close relationship; other types of osteoarthritis (post-traumatic, rheumatoid, metabolic, etc.); dysplastic syndromes and phenotypes; acute and chronic inflammatory diseases at the exacerbation stage; diabetes mellitus; osteoporosis; vascular diseases; obesity; malignant neoplasia; alcohol abuse. Along with clinical examination, the following laboratory methods were applied: immunological techniques, i.e., determination of TNFα, IL-1β, IL-4, IL-10; genetic testing using polymerase chain reaction, e.g., a point mutation of the IL4 gene at the 589(C>T) position, FCGR2A at 166(His>Arg) site, DEFB1 at the 20(G>A) and 52(G>A) positions. DNA from the peripheral blood of patients was used for the molecular genetic analysis. Radiographic examination was also carried out. The data were statistically processed using STATISTICA 6.1 software package (StatSoft, USA), Microsoft Office Excel 2019 for Windows 10. The differences were considered statistically significant at p ≤ 0.05. Results. The -589T/T genotype of IL4-589C>T gene polymorphism indirectly contributes to higher content of TNFα and IL-1β for primary osteoarthritis of the hip joints. The patients with -166Arg/Arg genotype have a 1.3-fold increase of certain cytokine concentrations, e.g., TNFα and IL-1β, as compared with -166His/Arg genotype, and, conversely, lower content of IL-4 and IL-10 (1.3- fold) in comparison with -166His/His genotype. The patients with -20A/A genotype showed higher levels of TNFα and IL-1β, respectively, 1.2 and 1.3 times, compared with -20G/G genotype, and 1.3 times versus the -20G/A genotype. Conclusions: 1. The presence of -589T/T genotype of the IL4-589C>T gene polymorphism and the -20A/A genotype of the DEFB1-20G>A gene polymorphism contributes to a high content of TNFα and IL-1β in the blood serum, and the carriage of -166His/His FCGR2A-166His>Arg gene polymorphism is associated with both higher level of TNFα, IL-1β, and a low concentration of IL-4, IL-10. 2. Complex carriers of FCGR2A166HisArg x DEFB152AA x DEFB120AA x IL4589TT genotypes in the patients with primary coxarthrosis increases the contents of TNFα, IL-1β cytokines by 1.5 and 1.7 times, respectively.

Individual and Combined Assessment of Ser680Asn FSH Receptor and FSHβ -211 G>T Gene Polymorphisms in Ovarian Response in IVF/ICSI Program

2020 ◽  
Vol 21 ◽  
Author(s):  
Elli Anagnostou ◽  
Alexia Kafkoutsou ◽  
Despina Mavrogianni ◽  
Ekaterini Domali ◽  
Evangelia Dimitroulia ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Gene Polymorphisms ◽  
Ovarian Response ◽  
Greek Population ◽  
Control Group ◽  
Genotype Distribution ◽  
Β Subunit ◽  
Molecular Genetic Study ◽  
The Impact ◽  
First Time

Background: Molecular biology tools, such as the detection of single nucleotide polymorphisms (SNPs), have been considered to assist to the management of the ovarian stimulation protocols. Purpose: The aim of this study was to evaluate the impact of two polymorphisms, the Asn680Ser polymorphism of the FSHR gene, and the FSH β subunit (FSHβ) gene polymorphism -211 G>T, in a Greek population of women undergoing IVF/ICSI program in our center. In addition, a control group of fertile women was studied, to verify whether there are differences in the genotype distribution between fertile and infertile population for both polymorphisms, as the FSHβ gene polymorphism -211 G>T is studied for the first time in the Greek population. Results : The FSH β-211 G>T polymorphism, studied for the first time in the Greek infertile population, appears to be quite rare. When studying the two polymorphisms separately, statistically significant differences were obtained that concerned the LH levels. Discussion: According to the combination analysis of the two polymorphisms by the number of alleles, women with 2-3 polymorphic alleles needed more days of stimulation, but there were no differences in pregnancy rates. Conclusion: This molecular genetic study helps to elucidate whether the polygenic combination of the Asn680Ser and FSH β subunit -211 G>T gene polymorphisms is of additive value in the prediction of ovarian response to exogenous gonadotropins.

scholarly journals The clinical value of ficolin-3 gene polymorphism in rheumatic heart disease. An Egyptian adolescents study

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Maher H. Gomaa ◽  
Emad Gamil Khidr ◽  
Ahmed Elshafei ◽  
Hala S. Hamza ◽  
Aya M. Fattouh ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Heart Disease ◽  
Gene Polymorphism ◽  
Rheumatic Heart Disease ◽  
Streptococcus Pyogenes ◽  
Gene Polymorphisms ◽  
Clinical Value ◽  
Homozygous Genotype ◽  
First Time ◽  
Rheumatic Heart

Abstract Objective Ficolin-3 is one of the innate immunity molecules that was thought to play a pivotal role in Streptococcus pyogenes autoimmunity and its complications; rheumatic fever (RF) and rheumatic heart disease (RHD). We aimed to disclose if there is an association between ficolin-3 (FCN3) gene polymorphisms (rs4494157 and rs10794501) and RF with or without RHD for the first time in Egyptian adolescents. Results Serum ficolin-3 level was significantly elevated in patients suffering from RF with and without RHD in comparison with control. Regarding FCN3 gene (rs4494157) polymorphism, a significant correlation was found between the A allele and the susceptibility to RF with or without RHD (OR = 2.93, P = 0.0002 and OR = 2.23, P = 0.008 respectively). Besides, AA homozygous genotype showed a significant association with RHD risk (OR = 3.47, P = 0.026). Patients carrying the A allele (CA + AA) had significantly higher serum ficolin-3 than those carrying the CC genotype (P ˂ 0.0001). While the frequency of (rs10794501) polymorphism revealed no significant differences between the controls and RF patients with or without RHD (OR = 1.43, P = 0.261 and OR = 1.48, P = 0.208 respectively).

Relative risk for cardiovascular morbidity in hemodialysis patients regarding gene polymorphism for IL-10, IL-6, and TNF

2016 ◽  
Vol 94 (10) ◽  
pp. 1106-1109 ◽  
Author(s):  
J. Tosic Dragovic ◽  
J. Popovic ◽  
P. Djuric ◽  
A. Jankovic ◽  
A. Bulatovic ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Cerebrovascular Accident ◽  
Gene Polymorphisms ◽  
Cardiovascular Events ◽  
Medical Center ◽  
Hemodialysis Patients ◽  
Left Ventricular ◽  
Cardiovascular Morbidity ◽  
Number Of Patients ◽  
Tnf Gene

Uremia-related inflammation is prone to be a key factor to explain high cardiovascular morbidity in hemodialysis patients. Genetic susceptibility may be of importance, including IL-10, IL-6, and TNF. The aim was to analyze IL-10, IL-6, and TNF gene polymorphisms in a group of hemodialysis patients and to correlate the findings with cardiovascular morbidity. This study included 169 patients on regular hemodialysis at Zvezdara University Medical Center. Gene polymorphisms for IL-10, IL-6 and TNF were determined using PCR. These findings were correlated with the cardiovascular morbidity data from patient histories. Heterozygots for IL-10 gene showed significantly lower incidence of cardiovascular events (p = 0.05) and twice lower risk for development of myocardial infarction, but experienced twice higher risk for left ventricular hypertrophy. Regarding TNF gene polymorphism, patients with A allele had 1.5-fold higher risk for cerebrovascular accident and cardiovascular events and 2-fold higher risk for hypertension and peripheral vascular disease. Patients with G allele of IL-6 gene experienced 1.5-fold higher risks for cerebrovascular accident. We need studies with larger number of patients for definitive conclusion about the influence of gene polymorphisms on cardiovascular morbidity in hemodialysis patients and its importance in everyday clinical practice.

scholarly journals The Influence of ACE Insertion/Deletion Gene Polymorphism on the Risk of IgA Nephropathy: A Debatable Topic

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Fen-Fen Chu ◽  
Shi-Kun Yang ◽  
Wen-Li Zeng
Keyword(s):  
Gene Polymorphism ◽  
Angiotensin Converting Enzyme ◽  
Iga Nephropathy ◽  
Gene Polymorphisms ◽  
Pooled Analysis ◽  
Chinese Patients ◽  
Converting Enzyme

Background. The connection between angiotensin-converting enzyme insertion/deletion (ACE I/D) gene polymorphisms and IgA nephropathy (IgAN) was conflicting. This pooled analysis was performed to explore this issue. Methods. All eligible investigations were identified from various electronic databases, and the pooled analysis was evaluated using Stata software. Results. 27 studies with 2538 IgAN cases and 3592 controls were included. In overall subjects, ACE D allele, DD, and II genotype were associated with IgAN susceptibility (D vs. I: OR = 1.21, 95% CI: 1.10–1.32, P < 0.001 ; DD vs. ID + II: OR = 1.38, 95% CI: 1.20–1.60, P < 0.001 ; and II vs. DD + ID: OR = 0.83, 95% CI: 0.73–0.95, P = 0.007 ). In Asian and Chinese patients, ACE I/D gene polymorphism was also correlated with IgAN vulnerability. Moreover, ACE D allele, DD, and II genotype were correlated with the progression of IgAN (D vs. I: OR = 1.37, 95% CI: 1.09–1.73, P = 0.008 ; DD vs. ID + II: OR = 1.57, 95% CI: 1.06–2.31, P = 0.024 ; and II vs. DD + ID: OR = 0.69, 95% CI: 0.49–0.99, P = 0.045 ). Conversely, in Caucasian subjects, there was no link between ACE I/D gene polymorphism and the risk of IgAN. Conclusion. ACE I/D gene polymorphism was correlated with the vulnerability and progression of IgAN in Asian and Chinese patients, and ACE D allele and DD homozygote genotype could be adverse factors for IgAN, while the II homozygote genotype could be an advantage factor. But, no significant association was found between ACE I/D gene polymorphism and IgAN in Caucasians.

scholarly journals Creation of new tomato forms with fungal disease resistance genes based on marker selection

2021 ◽  
pp. 16-21
Author(s):  
I. N. Shamshin ◽  
E. V. Grosheva ◽  
M. V. Maslova ◽  
R. M. Samoilova
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Fungal Diseases ◽  
Heterozygous State ◽  
Standard Type ◽  
Marker Selection ◽  
Young Leaves ◽  
Hybrid Forms

Relevance. The presented studies are aimed at obtaining new forms of tomato with a complex of genes for resistance to fungal diseases in combination with a standard type of bush and dark coloring of fruits based on marker-mediated selection.Methodology. The biological objects of the study are varieties and hybrid forms of tomato from the collection of the Michurinsky SAU. Molecular genetic analysis was performed using the following methods. DNA extraction was carried out from young leaves using a kit for isolation of NC Sample NC manufactured by Agrodiagnostika LLC according to the manufacturer's protocol. Fermentas production kits were used for PCR. Identification of the cladosporosis resistance gene was Cf-19 performed using the DNA marker R7. The presence of a fusarious wilting resistance gene was determined by a I-2/5 marker. The amplification results were visualized by agarose gel electrophoresis.Results. During the research, a collection of varieties and hybrid forms of tomato of the Michurinsky GAU was analyzed in order to identify genes for resistance to cladosporiosis Cf-19 and fusarium wilt I-2. A total of 52 genotypes were analyzed. It was found that most samples (41 samples) are characterized by a heterozygous state of the Cf-19 gene. All indeterminant and semi-determinant forms had both alleles. Of the 23 determinant forms presented in the collection, 10 had only one allele corresponding to recessive homozygote. Among all analyzed tomato genotypes, no dominant homozygous forms were noted. The study of the collection revealed several alleles of the I-2 gene. In total, four fragments corresponding to various alleles were amplified. A total of 50 resistant genotypes have been identified in the collection. Two alleys of the I-2 gene (633/693 bp) were identified in 42 tomato samples. Four varieties are homozygous in one allele (633 bp), which determines resistance. Three varieties have a second resistance allele (566 bp). One genotype has only an allele defining susceptibility (693 bp). On the basis of molecular analysis, as well as an assessment of the type of bush and fetal color, initial forms were selected with subsequent hybridization. 67 hybrid tomato plants were obtained. Evaluation of the presence of resistance genes showed that most of the resulting hybrids are resistant to cladosporiosis and fuzariosis. This is due to the presence of dominant alleles of Cf-19 and I-2 genes in a heterozygous state. Among the resulting hybrids, plants with a bark type of bush were identified. A total of 13 such plants were obtained.Conclusion. Thus, the work carried out allowed to obtain hybrid forms of tomato combine the signs of resistance to two pathogens of fungal diseases and the stem type of the bush. These forms are planned to be used in further selection work.

scholarly journals DNA markers of AHAS1 gene for screening and identification of sunflower genotypes of hybrid origin

2018 ◽  
Vol 23 ◽  
pp. 126-130
Author(s):  
A. Ye. Solodenko
Keyword(s):  
Dna Markers ◽  
Molecular Genetic ◽  
Pcr Amplification ◽  
Hybrid Origin ◽  
Molecular Genetic Study ◽  
Marker Selection ◽  
Hybrid Plants ◽  
Screening And Identification ◽  
Donor Line ◽  
Genetically Determined

Aim. A molecular genetic study of sunflower F1 and F2 hybrids on a microsatellite markers of mutant AHAS1 gene associated with herbicide resistance was performed. The aim of the work was to screen of F1 and F2 sunflower hybrid populations with usage of DNA markers and identification of homozygous segregants containing of the gene for resistance to SU herbicides. Methods. It was used PCR amplification to detect alleles of microsatellite locus located within the mutant AHAS1 gene. Results. The possibility of identification of hybrid plants with different alleles of the AHAS1 gene was shown. The efficiency of the allele of 191 b. p. in the homo- and heterozygous state for marker selection of genotypes resistant to the herbicide of the sulfonylurea group was confirmed. 10 homozygous F2 plants of SURES-2 x OS 1019B and 18 homozygous F2 plants of SURES-2 x OS 1029B, which according to the genotype correspond to the donor line of the mutant AHAS1 gene, were obtained. Conclusions. F2 plants that are carriers of this gene in the homozygous state can be used as an initial material in breeding for the purpose of creating new inbred lines with genetically determined resistance to SU herbicides. Keywords: DNA markers, AHAS1 gene, sunflower, herbicides, resistance.

scholarly journals Association of gdf9 Gene Polymorphisms With Litter Size in Indigenous Sheep of Bangladesh

2020 ◽  
Vol 7 (2) ◽  
pp. 283-292
Author(s):  
Faruk Hossain ◽  
Sharmin Akter Suma ◽  
Mohammad Shamsul Alam Bhuiyan
Keyword(s):  
Gene Polymorphism ◽  
Litter Size ◽  
Gene Polymorphisms ◽  
Climatic Conditions ◽  
Animal Protein ◽  
Selection Program ◽  
Indigenous Sheep ◽  
Gdf9 Gene

Sheep in Bangladesh are well known as efficient users of low quality roughages, welladapted to hot and humid agro-climatic conditions, capable of bi-annual lambing withmultiple births and resistant to common prevalent diseases. They play an important role inthe supply of animal protein. Present study aimed to investigate possible association ofGDF9 gene polymorphisms with litter size. A total of 126 DNA samples extracted from theblood of indigenous sheep from 5 locations namely Tangail, Noakhali, Naogaon,Gaibandha and Satkhira with known litter size were used to study the association ofGDF9 polymorphism by PCR based RFLP method. Two polymorphic regions of GDF9(FecG1 and FecG8) were amplified by PCR, digested with respective restriction enzymesand 126 sheep were genotyped. Current study revealed that genotype and allelefrequency for FecG1 varied among the sheep from different locations. The genotype (GG,AG, AA) frequency were 51.59%, 45.24% and 3.17% and the allele (G and A) frequenciesin the overall population were 74.21% and 25.79%, respectively. There was a significantassociation of FecG1 of GDF9 gene polymorphism with litter size. The homozygous GGgenotype had the lowest litter size (1.59±0.09; n=65) and homozygous AA genotype hadthe highest litter size (2.00±0.41; n=4). No genotypic variations were found for FecG8.Findings of this study specially the polymorphism of FecG1 together with genotyping ofsome sheep could be utilized in the selection program to increase the lamb productionpotentiality of indigenous sheep of Bangladesh. Res. Agric., Livest. Fish.7(2): 283-292,  August 2020

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