scholarly journals Chemical composition and antibacterial activity of methanolic extract from Echinops robustus on typical food-borne pathogens

2020 ◽  
Vol 9 (1) ◽  
pp. 849-852
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Bacillus Cereus ◽  
Methanolic Extract ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Gram Positive ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Typical Food

This study was conducted to evaluate the chemical composition and antibacterial activity of methanolic extract from Echinops robustus on typical food-borne pathogens. Chemical composition of the extract analyzed by gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis of the extract revealed 54 compounds in which Butanal (2.222%), 4-Heptanone (1.717%) and Palmitic acid (4.799%) were the major constituents. The agar disk diffusion method was used to study the antibacterial activity of Echinops robustus methanolic extract against 5 bacterial strains. The extract of Echinops robustus showed moderate antibacterial activity against two gram-positive and three gram negative microorganisms tested with higher sensitivity for gram positive ones (Clear zone= 10mm for Bacillus cereus and 12mm for Staphylococcus aureus). Minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were quantified by micro-dilution method. The MIC was 25 mg/ml for Bacillus cereus and 58 mg/ml for Escherichia coli and the MBC values were 400 and >400 mg/ml respectively for the mentioned species. Overall, results presented here suggest that the methanolic extract of Echinops robustus possesses antibacterial properties, and is, therefore, a potential source of active ingredients for food and pharmaceutical industry.

In-vitro Antioxidant and Antibacterial Activity of Methanolic Extract of Shorea robusta Gaertn. F. Resin

2016 ◽  
Vol 6 (4) ◽  
pp. 68
Author(s):  
Sushma Vashisht ◽  
Manish Pal Singh ◽  
Viney Chawla
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Positive ◽  
Gram Negative ◽  
Shorea Robusta ◽  
Dose Dependent ◽  
Resin Extract

The methanolic extract of the resin of Shorea robusta was subjected to investigate its antioxidant and antibacterial properties its utility in free radical mediated diseases including diabetic, cardiovascular, cancer etc. The methanol extract of the resin was tested for antioxidant activity using scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazil) radical method, reducing power by FeCl3 and antibacterial activity against gram positive and gram negative bacteria using disc diffusion method. The phytochemical screening considered the presence of triterpenoids, tannins and flavoniods. Overall, the plant extract is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging. The half inhibition concentration (IC50) of resin extract of Shorea robusta and ascorbic acid were 35.60 µg/ml and 31.91 µg/ml respectively. The resin extract exhibit a significant dose dependent inhibition of DPPH activity. Antibacterial activity was observed against gram positive and gram negative bacteria in dose dependent manner.Key Words: Shorea robusta, antioxidant, antibacterial, Disc-diffusion, DPPH.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Espeletia nana

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Alexis Peña ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Libia Alarcón ◽  
José Gregorio Baptista ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).

scholarly journals Phytochemical and Antibacterial Properties of Ethanolic Seed Extracts of Chrysophyllum albidum (African Star Apple)

2016 ◽  
Vol 1 (1-2) ◽  
pp. 05-09 ◽  
Author(s):  
Samuel L. Oputah ◽  
Kolawole O. Ajanaku ◽  
Raphael C. Mordi ◽  
Joseph A. O. Olugbuyiro ◽  
Shade J. Olorunshola ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Test Organisms ◽  
Seed Extracts ◽  
African Star Apple

Phytochemical and antibacterial properties of ethanolic extract of the seeds of African Star Apple (Chrysophyllum albidum) were investigated. The phytochemical result revealed the presence of saponins, carbohydrates, flavonoids, quinones, cardiac glycosides, fatty acids and terpenoids. The antibacterial activity was studied using agar well diffusion method at different concentrations against six pathogenic bacterial strains, three Gram-positive (Staphylococcus aureus, Micrococcus varians and Bacillus cereus) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris). Significant inhibitory activities were exhibited by the ethanolic seed extracts for all test organisms except Bacillus cereus. Zone of inhibition of the crude ethanolic extract was correlated with that of a standard antibiotic Gentamicin, for antibacterial activity. The results indicated a notable inhibition of the bacterial growth.

scholarly journals Evaluation of antibacterial efficacy of Ocimum sanctum L. against certain bacterial species and phytochemical analysis

2021 ◽  
Vol 22 (3) ◽  
pp. 341-345
Author(s):  
Aparajita Gupta ◽  
Mandeep Kaur
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Aqueous Extract ◽  
Methanolic Extract ◽  
Bacterial Species ◽  
Diffusion Method ◽  
Ocimum Sanctum ◽  
Phytochemical Analysis ◽  
Antibacterial Efficacy ◽  
Minimum Value

In the present study antibacterial efficacy of  aqueous and methanol extract obtained from leaves and stem of Ocimum sanctum L. was evaluated against certain test bacterial species viz. Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and Bacillus cereus. It is a traditional medicinal herb, commonly used for the treatment of various human health disorders.  The antibacterial activity was determined by using agar well diffusion method. Among the solvent extracts tested, aqueous extract of leaves exhibited higher antibacterial activity as compared to methanolic extract of leaves. Maximum antibacterial activity was recorded 17.6 mm against Bacillus cereus and minimum value was recorded 14 mm against S. aureus. Maximum antibacterial efficacy of O. sanctum leaves was found 7.3mm against P. aeruginosa and minimum value was 5mm against B. cereus in methanolic extract. Similar trend has been observed in extract of stem of O. sanctum in aqueous and methanolic solvent. The antibacterial efficacy has been found nil in methanolic extract. However in aqueous extract moderate activity has been recorded i.e. 9.3 mm against E.coli and minimum 7.3 mm against K. pneumoniae. The trend of phytochemicals revealed non significant variation. Phytochemical analysis of leaves and stem extracts of O. sanctum revealed the presence of various phytoconstituents i.e. alkaloids, flavonoids, saponins, tanins, glycosides.

scholarly journals Identification and Antibacterial Characterization of Endophytic Fungi from Artemisia sieberi

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Ibtisam Mohammed Ababutain ◽  
Sahar Khamees Aldosary ◽  
Amal Abdulaziz Aljuraifani ◽  
Azzah Ibrahim Alghamdi ◽  
Amira Hassan Alabdalall ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Molecular Identification ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Rrna Gene ◽  
Gram Positive ◽  
Artemisia Sieberi

Endophytic fungi serve as a reservoir for important secondary metabolites. The current study focused on the antibacterial properties of endophytic fungi isolated from Artemisia sieberi. Initially, six endophytic fungi were isolated and purified from the stem of A. sieberi. Endophytic fungi were identified by morphological characteristics, as well as by molecular identification using 18S rRNA gene sequencing method. All the six isolates were subjected to the preliminary screening for their antibacterial activity against nine important pathogenic bacteria using the disk-diffusion method. Crude extracts of the most active isolate were obtained using ethyl acetate. Antibacterial activity of the ethyl acetate extract was evaluated using well diffusion method on the selected isolate. The antibacterial efficiency of the selected isolate was evaluated by determining the Minimum Inhibitory Concentration (MIC). MIC values were in appreciable quantity against both Gram-positive and Gram-negative bacteria ranging from 3.125 to 6.25 µg/mL and 12.5 to 50 µg/mL, respectively. This result indicated that Gram-positive bacteria were more susceptible to the endophytic fungi extract. Moreover, the molecular identification results revealed that all the isolates belong to Ascomycota and represented Aspergillus and Penicillium genera and three species: A. oryzae (three isolates), A. niger (one isolate), and P. chrysogenum (two isolates). All six endophytic fungi were able to inhibit the growth of at least two of the tested bacteria. Among the isolated strains, isolate AS2, which identified as P. chrysogenum, exhibited the highest antibacterial activity against all nine tested bacteria and was higher than or equal to the positive control against most of the tested bacteria. Future studies are required to isolate and identify these bioactive substances, which can be considered as a potential source for the synthesis of new antibacterial drugs to treat infectious diseases.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil from Qiancao (Rubia cordifolia Linn.) Roots against Selected Foodborne Pathogens

2019 ◽  
Vol 7 (4) ◽  
Author(s):  
Wei-Qin Li ◽  
Mei-Ping Quan ◽  
Qin Li
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Foodborne Pathogens ◽  
Microbial Growth ◽  
Inhibitory Concentration ◽  
Gas Chromatography Mass Spectrometry ◽  
Natural Antimicrobial ◽  
Food Borne Pathogens ◽  
Food Borne

Chemical composition of the essential oil from Qiancao roots was analyzed by gas chromatography-mass spectrometry (GC-MS), and 43 components were identified in the paper. Mollugin (30.58%) was found to be the major component, followed by 3-methyl-2-cyclopenten-1-one (10.55%), 4-tert-butyl-2-phenyl-phenol (8.64%) and eugenol (6.30%). The antibacterial activity of essential oil against selected food-borne pathogens was evaluated. The results showed that the sensitivity to essential oil were different for different tested bacteria and the essential oil had a strong antibacterial activity against B. cereus with the lowest minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) values of 0.0625 and 0.125 mg/mL, respectively. In addition, microbial growth curve assay also showed that the essential oil had a significant effect on the growth rate of surviving B. cereus. The data of this study suggests that the essential oil from Qiancao roots has great potential for application as a natural antimicrobial agent to preserve food.

scholarly journals Antibacterial Activity of Clay Soils against Food-Borne Salmonella Typhimurium and Staphylococcus aureus

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 170
Author(s):  
Nur Naqiyah Azmi ◽  
Nor Ainy Mahyudin ◽  
Wan Hasyera Wan Omar ◽  
Nor-Khaizura Mahmud Ab Rashid ◽  
Che Fauziah Ishak ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Salmonella Typhimurium ◽  
Metal Ion ◽  
Antimicrobial Agents ◽  
Antibacterial Properties ◽  
Xrd Analysis ◽  
Diffusion Method ◽  
Food Borne ◽  
And Staphylococcus Aureus

Natural clays have recently been proven to possess antibacterial properties. Effective natural antimicrobial agents are needed to combat bacterial contamination on food contact surfaces, which are increasingly more prevalent in the food chain. This study sought to determine the antibacterial activity of clays against the food-borne pathogens Salmonella Typhimurium ATCC 14028 and Staphylococcus aureus ATCC 13565. Soils were processed to yield leachates and suspensions from untreated and treated clays. Soil particle size, pH, cation-exchange capacity, metal composition and mineralogy were characterized. Antibacterial screening was performed on six Malaysian soils via the disc diffusion method. In addition, a time-kill assay was conducted on selected antibacterial clays after 6 h of exposure. The screening revealed that Munchong and Carey clays significantly inhibit Salmonella Typhimurium (11.00 ± 0.71 mm) and S. aureus (7.63 ± 0.48 mm), respectively. Treated Carey clay leachate and suspension completely kill Salmonella Typhimurium, while S. aureus viability is reduced (2 to 3 log10). The untreated Carey and all Munchong clays proved ineffective as antibacterials. XRD analysis confirmed the presence of pyrite and magnetite. Treated Carey clays had a higher soluble metal content compared to Munchong; namely Al (92.63 ± 2.18 mg/L), Fe (65.69 ± 3.09 mg/L) and Mg (88.48 ± 2.29 mg/L). Our results suggest that metal ion toxicity is responsible for the antibacterial activity of these clays.

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