scholarly journals Antibacterial Activity of Clay Soils against Food-Borne Salmonella Typhimurium and Staphylococcus aureus

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 170
Author(s):  
Nur Naqiyah Azmi ◽  
Nor Ainy Mahyudin ◽  
Wan Hasyera Wan Omar ◽  
Nor-Khaizura Mahmud Ab Rashid ◽  
Che Fauziah Ishak ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Salmonella Typhimurium ◽  
Metal Ion ◽  
Antimicrobial Agents ◽  
Antibacterial Properties ◽  
Xrd Analysis ◽  
Diffusion Method ◽  
Food Borne ◽  
And Staphylococcus Aureus

Natural clays have recently been proven to possess antibacterial properties. Effective natural antimicrobial agents are needed to combat bacterial contamination on food contact surfaces, which are increasingly more prevalent in the food chain. This study sought to determine the antibacterial activity of clays against the food-borne pathogens Salmonella Typhimurium ATCC 14028 and Staphylococcus aureus ATCC 13565. Soils were processed to yield leachates and suspensions from untreated and treated clays. Soil particle size, pH, cation-exchange capacity, metal composition and mineralogy were characterized. Antibacterial screening was performed on six Malaysian soils via the disc diffusion method. In addition, a time-kill assay was conducted on selected antibacterial clays after 6 h of exposure. The screening revealed that Munchong and Carey clays significantly inhibit Salmonella Typhimurium (11.00 ± 0.71 mm) and S. aureus (7.63 ± 0.48 mm), respectively. Treated Carey clay leachate and suspension completely kill Salmonella Typhimurium, while S. aureus viability is reduced (2 to 3 log10). The untreated Carey and all Munchong clays proved ineffective as antibacterials. XRD analysis confirmed the presence of pyrite and magnetite. Treated Carey clays had a higher soluble metal content compared to Munchong; namely Al (92.63 ± 2.18 mg/L), Fe (65.69 ± 3.09 mg/L) and Mg (88.48 ± 2.29 mg/L). Our results suggest that metal ion toxicity is responsible for the antibacterial activity of these clays.

scholarly journals Pharmacological Role of Anions (Sulphate, Nitrate, Oxalate and Acetate) on the Antibacterial Activity of Cobalt(II), Copper(II) and Nickel(II) Complexes With Nicotinoylhydrazine-Derived ONO, NNO and SNO Ligands

1996 ◽  
Vol 3 (5) ◽  
pp. 211-217 ◽  
Author(s):  
Zahid Hussain Chohan ◽  
Abdur Rauf
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Metal Ion ◽  
Bacterial Species ◽  
Biologically Active ◽  
Schiff Base Ligands ◽  
Mixed Ligands ◽  
And Staphylococcus Aureus

Mixed ligands biologically active complexes of cobalt(II), copper(II) and nickel(II) with nicotinoylhydrazine-derived ONO, NNO and SNO donor schiff-base ligands having the same metal ion but different anions such as sulphate, nitrate, oxalate and acetate have been synthesised and characterised on the basis of their physical, analytical and spectral data. In order to evaluate the role of anions on their bioability, these ligands and their synthesised metal complexes with various anions have been screened against bacterial species such as Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus and the title studies have proved a definative role of anions in increasing the biological activity

scholarly journals The Antibacterial Properties of Bayur Tissues’ Extract (Pterospermum subpeltatum C.B. Rob)

2014 ◽  
Vol 69 (5) ◽  
Author(s):  
Pince Salempa ◽  
Alfian Noor ◽  
Tjodi Harlim ◽  
Nunuk Hariani ◽  
Muharram Muharram ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Traditional Medicine ◽  
Secondary Metabolite ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Shigella Boydii ◽  
And Staphylococcus Aureus ◽  
Antibacterial Potential

Pterospermum is of plant genus in the Sterculiaceae family, which has never been studied for its secondary metabolite compound. The others species from this genus have long been used in generations of traditional medicine. For example, P. Javanicum has been used to treat dysentery, toothaches and ulcers. From the antibacterial testing carried out on some tissues of Pterospermum subpeltatum by GNA diffusion method,  this plant showed antibacterial potential, especially toward Shigella boydii and Staphylococcus aureus.

scholarly journals Antibacterial activity of different extract of medicinal plant Antirrihnum Orontium.

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Himayat Ullah ◽  
Shehzad Saleem ◽  
Wisal Ahmad ◽  
Muhammad Hanif ◽  
Zahid Ali ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Water Extract ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Water Extracts ◽  
The Family ◽  
Quaternary Base ◽  
And Staphylococcus Aureus

Antirrhinum Orontium is belonging to the family Plantaginacea commonly known as lesser snapdragon which is a flowering plant belong to genus Antirrhinum. It was traditionally used in treatment of Tumor, Eye inflammation and Ulcer. Antirrhinum Orontium is contain mostly tertiary alkaloids bases one of them is identified as 4-methyl-2, 6-naphthyridine and Choline is identified as quaternary base. The recent study is to investigate the antibacterial activity of Ethyl acetate, Dichloromethane, Hexane and Water extracts of Antirrhinum Orontium, against the pathogen like Escherichia coli and Staphylococcus aureus. Both the Soxhlet and Maceration water extract were found most active from the other extract against Escherichia coli and Staphylococcus aureus by using agar well diffusion method. The measured zone of inhibition for Soxhlet water extract is 21±0.05mm and Maceration water extract is 20±0.05mm against gram positive Staphylococcus aureus, while for gram negative Escherichia coli 23±0.05mm is measured for Soxlet and 21±0.05mm measured for Maceration water extracts

scholarly journals Combination Gel Formulation Extracts of Moringa Leaf and Red Betel Leaf as an Inhibitor of Acne-Causing Bacteria (Propionibacterium acne and Staphylococcus aureus)

2021 ◽  
Vol 8 (3) ◽  
pp. 18-23
Author(s):  
Eko Sri Wahyuningsih ◽  
Wahono Sumaryono ◽  
Chaidir Chaidir
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Chemical Parameters ◽  
Physical And Chemical Parameters ◽  
Gel Preparation ◽  
Physical And Chemical ◽  
And Storage ◽  
And Staphylococcus Aureus

The most common skin disorder is acne (Acne vulgaris) which is a chronic inflammatory disease that occurs in the pilosebaceous unit. Infection can be caused by Propionibacterium acnes and Staphylococcus aureus. Moringa leaves are known to inhibit acne growth. Piper crocatum leaves are also thought to have antibacterial activity. The study aims to determine the effect of the combination of the two extracts in inhibiting the growth of acne-causing bacteria, also tested the gel formula to meet physical and chemical parameters as well as stability to temperature and storage, also to test the acute dermal irritation against rabbit. Each extract with a concentration of 0.3%; 0.6%; 1.25%; 2.5%; 5%; 10%; 20%, incubated then measured the inhibition zone, then determined the minimum inhibition zone concentration of the two extracts to determine the lowest levels of the extracts which still gave antibacterial activity against the tested bacteria by the well diffusion method. The extract combination was formulated in a gel preparation with the excipients Carbopol 940, PG, Phenoxyethanol, TEA, CMC, and Aquadest. Antibacterial activity test for gel preparations used the well method with positive control Medi-Klin® gel. Then the formula evaluation includes physical, chemical, and microbiological evaluations. The results showed that the combination gel form had activity against P. acne and S. aureus at concentrations of 2.5% : 2.5%. The combination gel preparation of the two extracts also has synergistic effect in inhibiting acne bacteria. The formula can meet physical and chemical parameters and is stable to temperature and storage.

scholarly journals ANTIBACTERIAL ACTIVITY OF Samanea saman LEAF ETHANOL EXTRACT AGAINST Escherichia coli AND Staphylococcus aureus AND ITS TOTAL FLAVONOID AND PHENOLIC CONTENTS

Jurnal Kimia ◽  
2018 ◽  
pp. 121
Author(s):  
W. S. Rita ◽  
I M. D. Swantara ◽  
I. A. R. Astiti Asih ◽  
N.K. Sinarsih
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
E Coli ◽  
Phenolic Contents ◽  
Agar Disc ◽  
Samanea Saman ◽  
And Staphylococcus Aureus

Antibacterial activity of Samanea saman usually shows a positif correlation to the flavonoid and phenolic contents.  The aim of this study was to evaluate the antibacterial activity of the ethanol extract of Samanea saman against Escherechia coli and Staphylococcus aureus and determine the total flavonoid and phenolic contents of the extract. The extraction was done by ethanol 96% at room temperature. The antibacterial assay was conducted by agar disc diffusion method. The total flavonoid and phenolic contents were determined by UV-Vis Spectrofotometer with the standard of quersetin and galic acid, respectively. The extraction of 250 g of Samanea saman leaves resulted in 24.5 g of ethanol extracts. The ethanol extract showed a moderate inhibition of 8.33 mm towards E. coli and a strong inhibition of 13.6 mm towards S. aureus at the concentration of 4%. The Minimum Inhibitory Concentration (MIC) of the extract against E. coli and S.aureus were of 3% and 0.3%”, respectively. The total flavonoid and phenolic contents were successively 1233.2991 mg QE/100g and 2544.6154 mg GAE/100g. Keywords: Escherechia coli, flavonoid and phenolic content,  Samanea saman, Staphylococcus aureus

Evaluation of the Glucuronic Acid Production and Antibacterial Properties of Kombucha Black Tea

2019 ◽  
Vol 20 (11) ◽  
pp. 985-990
Author(s):  
Fereshteh Ansari ◽  
Hadi Pourjafar ◽  
Ali Kangari ◽  
Aziz Homayouni
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Salmonella Typhimurium ◽  
Acid Production ◽  
Glucuronic Acid ◽  
Storage Temperature ◽  
Antibacterial Properties ◽  
Lactobacillus Rhamnosus ◽  
Black Tea ◽  
The Difference

Background: Kombucha beverage is considered as a dietary supplement and drinking it strengthens the body’s immune system which prevents diseases. Objective: The purpose of this study was to determine the amount of glucuronic acid and antibacterial activity of Kombucha black tea drink during its production at different storage temperature. Methods: The extent of glucuronic acid at temperatures of 20°C and 30°C was explored by the use of the HPLC system for 21 days. To analyse the antibacterial property, the influence of Kombucha black tea supernatant on the growth of Salmonella typhimurium, Staphylococcus aureus, and Lactobacillus rhamnosus bacteria was examined via the two procedures of the disc and agar well diffusion. Results: The production of glucuronic acid underwent a variation at 20°C from 17.0 mg/L on day 1 to roughly 27.2 mg/L on day 21, and the difference was significant. Furthermore, the quantity of this acid at 30°C increased from 42.2 mg/L on day 1 to 48.0 mg/L on day 21. The amount of glucuronic acid produced at 30°C was significantly greater than that at 20°C (p<0.05). This study indicated that the Kombucha black tea has antibacterial activity against Salmonella typhimurium and Staphylococcus aureus, but not against Lactobacillus rhamnosus. However, there are no statistical differences in antibacterial activity of Kombucha between incubation at 20oC and 30oC (P>0.05). Conclusion: This study offers a perspective on glucuronic acid production (especially in 30°C rather than 20°C) and antibacterial activity of Kombucha black tea beverage.

scholarly journals Comparative evaluation of antibacterial activity of silver oxides with different oxygen content

2021 ◽  
pp. 23-28
Author(s):  
E.M. Gordina ◽  
◽  
S.A. Bozhkova ◽  
A.A. Erusin ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Oxygen Content ◽  
Optical Density ◽  
Antibacterial Properties ◽  
Nutrient Broth ◽  
Low Oxygen ◽  
Pure Silver ◽  
And Staphylococcus Aureus ◽  
Density Results

Aim of the study. To study the presence of antibacterial properties in silver oxides with different oxygen content in relation to sensitive and methicillinresistant Staphylococcus aureus strains. Material and Methods. Films of silver and its oxides were coated on paper surface in vacuum via the method of ion-plasma arc sputtering of the metallic target. Antibacterial activity of the obtained samples of silver oxides and pure silver against Staphylococcus aureus АТСС 29213 and Staphylococcus aureus ATCC 43300 was studied applying the diff usion method as well as in liquid nutrient broth with consequent measuring of optical density. Results. As a result of antibacterial activity investigation of the samples obtained via the diff usion method and liquid nutrient broth, it was revealed that all samples possessed antibacterial activity in relation to type strains of S. aureus. Th erewith, the effi cacy of the samples with pure silver was signifi cantly lower than that of silver oxides (Ag0,2O, Ag0,4O, Ag1O, Ag1,5O, Ag2O). It was shown that the activity of silver oxides did not depend on the oxygen concentration in them. Thus, statistically signifi cant diff erences in optical density of the nutrient medium with bacteria and the medium with incubated S. aureus АТСС 29213 (MSSA) and AgxO (р<0.001). Analogical results were obtained aft er studying of infl uence exerted by silver on S. aureus АТСС 43300 (MRSA). Conclusion. All silver oxides samples created were characterised by antibacterial activity against S. aureus. The data obtained point at the prospects in application of silver oxide with low oxygen content for prevention and treatment of Staphylococcus aureus strains infection.

scholarly journals ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF MANGIFERA INDICA ETHANOL AND AQUEOUS LEAVES EXTRACT AGAINST PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS

2021 ◽  
Vol 8 (12) ◽  
pp. 262-269
Author(s):  
M. A. Garga ◽  
U. M. Garasin ◽  
M. Abdullahi ◽  
B. A. Muhammed ◽  
A. Yakubu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Mangifera Indica ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Gram Staining ◽  
Significant Difference ◽  
Zones Of Inhibition ◽  
And Staphylococcus Aureus

The aim of this research is to investigate the antibacterial activity and identify the phytochemical constituents of Mangifera indica leafs on Pseudomonas aeruginosa and Staphylococcus aureus using disc diffusion method. The sample was collected fresh from the premises of Bioresources Development Center (BIODEC), Katsina, Katsina State and was dried and pounded into powder. The powdered leaves were extracted using ethanol and aqueous solvents. Various concentrations ranging from 500mg to 62.5mg were prepared. Test isolates were obtained from the Microbiology laboratory, Umaru Musa Yar’adua University Katsina (UMYUK) and were further authenticated using Gram staining and biochemical test. The bacterial inoculums were standardized to McFarland scale 0.5. Zones of inhibition were read after 24 hours at 370C. The results of the antibacterial study revealed that the ethanolic leaves extracts at 500mg/ml had effect on P. aeruginosa and S. aureus with zones of inhibition of 12mm and 6mm respectively. The results of the phytochemical screening revealed the presence of flavonoids, tannins, saponins, alkaloids and phenols where only alkaloids was found to be absent in the aqueous extract. There is no significant difference between the solvents and various concentrations used base on t-test data analysis.

scholarly journals In-Vitro Antibacterial Activity of crude Garlic (Allium Sativum) Extract Against Clinical Isolates of Methicillin Resistant Staphylococcus aureus

2021 ◽  
Vol 14 (3) ◽  
pp. 1449-1457
Author(s):  
Salma Osman Noorelhuda Mohammed ◽  
Nadir Musa Khalil Abuzeid ◽  
Sara Abdelghani ◽  
Lienda Bashier Eltayeb
Keyword(s):  
Staphylococcus Aureus ◽  
Allium Sativum ◽  
Antimicrobial Agents ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Negative Consequences ◽  
Methicillin Resistant ◽  
And Staphylococcus Aureus

Background:Methicillin-resistant Staphylococcus aureus (MRSA) has gained significant health solicitude globallydue to its resistance to nearly almost antimicrobial agents, and garlic is one of nature's most powerful antibiotics that must be used as a pharmaceutical regimen. The current study aimed to determine the In-Vitro antibacterial efficacy of crude garlic extract against MRSA. Methods: The aqueous and 70% ethanol crude garlic (Alllium sativum)extract was prepared. Disc diffusion method was performed to assess the antimicrobial activity for100 clinical isolates of MRSA collected,The reference standard strain was Staphylococcus aureus (ATCC 25923). Results: All MRSA strains assessed were significantly sensitive to 70% ethanolic extract at various concentrations range from 200 to 25%, exhibited inhibitory effects against clinical isolates and Staphylococcus aureus (ATCC 25923) with the means of inhibition zones ranging from 17.76- 14.35 mm and 15-13 mm in length, while the aqueous extracts were less in both clinical isolates and Staphylococcus aureus (ATCC 25923) ranging from 11.93-8.62 mm and 11-8 mm respectively, methanol and distilled water were not effected on growth. Conclusion: The findings demonstrate that 70%ethanol extract of crude Allium sativum has significantly inhibitory effect on methicillin-resistant Staphylococcus aureus is better than aqueous extract. This study does not undermine the value of antibiotic use, but instead the probability of using them in low dosage to minimize their negative consequences.

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