scholarly journals Green Synthesis of Silver Nanoparticles by Leaf Extracts of Boerhavia erecta and Spectral Characterization and Their Antimicrobial, Antioxidant ad Cytotoxic Studies on Ovarian Cancer Cell Lines

2020 ◽  
Vol 9 (3) ◽  
pp. 1165-1176
Keyword(s):  
Ovarian Cancer ◽  
Particle Size ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Cell Lines ◽  
Resonance Phenomenon ◽  
Moderate Activity ◽  
Leaf Extracts ◽  
Anticancer Properties ◽  
Wide Range

The selection of silver metal for the biosynthesis of nanoparticles is a great choice as it exhibits a wide range of applications. Boerhavia Erecta is an extraordinary plant species thatis useful and unique because of its physical characteristics and genetic composition. The current study reported the synthesis of silver nanoparticles by using B. Erecta leaf extract and spectral characterized and studied their properties to target ovarian cancer by antitumorigenic activity. Green synthesized Boerhavia Erecta silver nanoparticles (Be-AgNPs) exhibit the Surface Plasmon resonance phenomenon at 446 nm. The particle size of Be-AgNPs was found to be 15.9 ± 1 nm by particle size analyzer technique. The zeta potential of the biosynthesized Be-AgNPs found to be -36.9 mV, which is a high negative charge indicating its stability and polydispersed nature. The FT-IR results confirmed the presence of secondary metabolites and proteins around the green synthesized Be-AgNPs, which are responsible for the reduction and stability of the nanoparticles. The antioxidant study confirmed the high reflection of reduction property of oxygen free radicals, and the antibacterial activity showed moderate activity against both gram-positive and gram-negative microorganisms when compared with standard antibiotics by disc diffusion assay. Anticancer studies reveal that the biosynthesized Be-AgNPs were very effective and cytotoxic to ovarian cancer PA-1 cell line at the concentration of 25 µg/ml confirmed by performing MTT assay. It is concluded that the eco-friendly biosynthesized Be-AgNPs show evidence of a high range of anticancer properties against ovarian cancer PA-1 cell lines, antioxidant and moderate antibacterial activity. Hence the biosynthesized Be-AgNPs can be good source antioxidants, and anticancer agent’s in future therapeutic applications.

scholarly journals Antioxidant and cytotoxic studies of Acacia nilotica twig extract and their green synthesized silver nanoparticles

2020 ◽  
Vol 9 (2) ◽  
pp. 975-980 ◽  
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Cell Lines ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Acacia Nilotica ◽  
Total Phenolic ◽  
Hek 293 ◽  
Selected Area Electron Diffraction ◽  
Wide Range

Acacia nilotica (L.) Delile is well known as “Desi Kikar”or Babul in India that possesses a wide range of pharmacological activities. In the present study, Acacia nilotica twig extract and its synthesized silver nanoparticles (AgNPs) were evaluated for total phenolic content (TPC), antioxidant activity and cytotoxic effects. Characterization of AgNPs was done by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Selected area electron diffraction (SAED) techniques. Antioxidant potential was determined using different assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power and β-carotene linoleic acid. Cytotoxicity was tested by 3-(4,5-dimethyl-2-yl)-2,5-diphynyl tetrazolium bromide (MTT) assay on Human embryonic kidney (HEK)-293 cell lines. The results indicated that AgNPs exhibited higher antioxidant activity (81.11 %) and TPC (57.35 mg of GAE/mL of extract) as compare to plant extract. A positive correlation was observed between the TPC and antioxidant activities. The inhibitory concentration (IC50) of A. nilotica extract and AgNPs was 52.08µg/mL and 56.82µg/mL respectively. Cytotoxicity against HEK-293 cell lines was dose dependent. Accordingly, it is summarized that A. nilotica based AgNPs could serve as a potential antioxidant for therapeutic purposes.

scholarly journals Synthesis of zinc oxide and silver nanoparticles using ficus palmata - Forssk leaf extracts and assessment of antibacterial activity

2020 ◽  
Vol 26 (6) ◽  
pp. 200454-0
Author(s):  
Sabaoon Shamshad ◽  
Jamshaid Rashid ◽  
Ihsan-ul-haq ◽  
Naseem Iqbal ◽  
Saif Ullah Awan
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Inhibitory Concentration ◽  
Oxide Nanoparticles ◽  
Leaf Extracts ◽  
E Coli ◽  
Ficus Palmata

Multidrug resistance of bacteria is an emerging human health hazard and warrants development of novel antibacterial agents with more effective mode of action. Here, zinc oxide and silver nanomaterials were prepared using Ficus palmata Forssk leaf extract with efficient antibacterial activity. SEM coupled with EDS confirmed the spherical symmetry with average particle diameter 50 to 65 nm while the XRD confirmed crystalline face centered cubic structure of silver and hexagonal crystallize phase of zinc oxide nanoparticles. Antibacterial activity was evaluated for 8 pathogenic bacterial strains including 3 drug resistant pathogenic strains. The nanoparticles showed enhanced growth inhibition for resistant strains in comparison with the broad-spectrum antibiotics i.e. roxithromycin and cefixime. Minimum inhibitory concentration in μg.mL<sup>-1</sup> of silver nanoparticles was found to be as low as 33.3 for resistant Streptococcus haemolyticus; 11.1 for Staphylococcus aureus and E Coli; and 3.7 μg.mL<sup>-1</sup> for resistant Pseudomonas aeruginosa. Similarly, the minimum inhibitory concentration of zinc oxide nanoparticles was found to be 100 μg.mL<sup>-1</sup> against resistant Streptococcus haemolyticus and Staphylococcus aureus; 11.1 μg.mL<sup>-1</sup> for resistant Pseudomonas aeruginosa; and 3.7 μg.mL<sup>-1</sup> against resistant E coli. Ficus palmata Forssk leaf extracts can be explored effectively for synthesizing active antibacterial nanomaterials as a non-toxic and environmentally benign synthesis route.

Autoclave-assisted green synthesis of silver nanoparticles using A. fumigatus mycelia extract and the evaluation of their physico-chemical properties and antibacterial activity

2018 ◽  
Vol 7 (3) ◽  
pp. 217-224 ◽  
Author(s):  
Sarah Ghanbari ◽  
Hamideh Vaghari ◽  
Zahra Sayyar ◽  
Mohammad Adibpour ◽  
Hoda Jafarizadeh-Malmiri
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Chemical Properties ◽  
Heating Time ◽  
Gram Negative Bacteria ◽  
Synthesis Conditions ◽  
High Antibacterial Activity ◽  
Physico Chemical ◽  
Ft Ir

Abstract Silver nanoparticles (AgNPs) were synthesized using Aspergillus fumigatus (A. fumigatus) mycelia extract via the hydrothermal method. The main reducing and stabilizing groups and components of A. fumigatus extract, such as amine, hydroxyl, amid, protein, enzymes, and cell saccharide compounds, were identified by Fourier transform infrared (FT-IR). Central composition design was used to plan the experiments, and response surface methodology was applied to evaluate of the effects of independent variables, including the amount of the prepared extract (5–7 ml) and heating time (10–20 min) at 121°C and 1.5 bar), on the particle size of the synthesized AgNPs, as manifested in broad emission peak (λmax). More stable and spherical monodispersed AgNPs, with mean particle size, polydispersity index (PDI) value, and maximum ζ potential value of 23 nm, 0.270, and +35.3 mV, respectively, were obtained at the optimal synthesis conditions using 7 ml of A. fumigatus extract and heating time of 20 min. The synthesized AgNPs indicated high antibacterial activity against both Gram-positive and Gram-negative bacteria.

scholarly journals Antibacterial Activity of Guyabano, Ulasimang Bato, Sambong, and Tsaang Gubat Leaf Extracts against Common Drug-resistant Bacteria

2020 ◽  
Vol 54 (1) ◽  
Author(s):  
Cecilia C. Maramba-Lazarte ◽  
Lolita L. Cavinta ◽  
Ma. Carmelita L. Sara
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Resistant Bacteria ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Moderate Activity ◽  
Alcoholic Extract ◽  
Drug Resistant ◽  
Leaf Extracts ◽  
E Coli

Background. Using plants as antimicrobials has long been a practice of traditional healers and validating these customs may lead to the discovery and development of useful herbal medicines. Objective. This study aimed to determine the antibacterial activity of guyabano, tsaang gubat, sambong, and ulasimang bato against common pathogens. Methods. Aqueous or alcoholic leaf extracts of the different medicinal plants were prepared. The solid agar dilution method was used to determine the MIC of guyabano, tsaang gubat, sambong, and ulasimang bato against common pathogens including Staphylococcus aureus, Echerichia coli, Streptococcus pneumoniae, Hemophilus influenzae, Pseudomonas aeruginosa, Salmonella typhi, and Shigella flexneri. Results. The alcoholic leaf extract of guyabano showed moderate activity against oxacillin-sensitive S. aureus with an MIC of 5-6.3 mg/mL. However, tsaang gubat did not exhibit any antibacterial activity for drug-resistant enteric organisms (S. typhi, S. flexneri, and E. coli) and S. aureus at a concentration of 25 mg/mL. Even at a concentration of 100mg/mL, ulasimang bato failed to show any antibacterial activity against drug-resistant S. aureus, S. pneumonia, H. influenzae, E. coli, and P. aeruginosa. Sambong alcoholic extract had some antibacterial activity against penicillin-resistant S. pneumoniae with an MIC of 12.5 mg/mL. Conclusions. Guyabano alcoholic leaf extract showed moderate antibacterial activity against oxacillin-sensitive S. aureus. Sambong alcoholic extract likewise exhibited inhibitory activity against S. pneumoniae. However, tsaang gubat and ulasimang bato aqueous extracts failed to show significant antibacterial activity for the pathogens tested.

scholarly journals Antibacterial activity of silver nanoparticles of different particle size against Vibrio Natriegens

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0222322 ◽  
Author(s):  
Yaohua Dong ◽  
Hongling Zhu ◽  
Yuanyuan Shen ◽  
Wenting Zhang ◽  
Li Zhang
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Antibacterial Activity

scholarly journals Carbene modification and reversible crosslinking of silver nanoparticles for controlled antibacterial activity

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Liling Jing ◽  
Mark G. Moloney ◽  
Hao Xu ◽  
Lian Liu ◽  
Wenqiang Sun ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Particle Size ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Photoelectron Spectroscopy ◽  
Inhibition Zone ◽  
Insertion Reaction ◽  
Ag Nps ◽  
Zone Method ◽  
Transmission Electron

Abstract Silver nanoparticles (Ag NPs) system capable of exhibiting different particle size at different temperature was developed, which depended on the extent of Diels–Alder (DA) reaction of bismaleimide with furan. Thus, Ag NPs were functionalized on the surface by a furyl-substituted carbene through an insertion reaction. Subsequent reversible DA crosslinking achieved a controlled aggregation with different particle size, which gives a series of different antibacterial activity. These Ag NPs were characterized by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray Photoelectron Spectroscopy (XPS), and Nanoparticle Size Analyzer. The aggregation of the Ag NPs could be reliably adjusted by varying the temperature of DA/reverse-DA reaction. The antibacterial activity was assessed using the inhibition zone method against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), which decreased first and then increased in agreement with the size evolution of Ag NPs. This approach opens a new horizon for the carbene chemistry to modify silver nanoparticles with variable size and give controlled antibacterial activity.

scholarly journals Rapid micropropagation, antioxidant and antibacterial assays of Ocimum spp.

2020 ◽  
Vol 49 (3) ◽  
pp. 459-465
Author(s):  
Shamoly Akter ◽  
Barna Goswami ◽  
Salim Khan ◽  
Shahina Akter ◽  
Sanjida Rahman Mollika ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Root Formation ◽  
Shoot Induction ◽  
Leaf Extracts ◽  
In Vitro Micropropagation ◽  
Wide Range ◽  
Half Strength ◽  
Ocimum Tenuiflorum ◽  
High Inhibition

In vitro micropropagation was studied in two species of Ocimum and one species was used for antioxidant and antibacterial activity. The maximum number of shoots/explant (10.1 and 9.5) was induced in MS + 1.0 mg/l BAP + 0.5 mg/l Kn and MS + 2.0 mg/l BAP + 0.5 mg/l IAA in Ocimum tenuiflorum and O. americanum, respectively. GA3 (0.1 mg/l) was found to be effective for shoot induction and elongation in both the species. Strong and stout root formation was observed on half strength MS medium supplemented with 0.2 mg/l IBA for O. tenuiflorum and half strength MS + 0.5 mg/l NAA for O. americanum. Methanolic leaf extract of O. tenuiflorum showed high inhibition of DPPH activity compared to standard antioxidants like quercetin and the chloroform leaf extracts exhibited wide range of antibacterial activity.

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